MD791Z - Process for treating common wheat seeds with low viability - Google Patents
Process for treating common wheat seeds with low viability Download PDFInfo
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- MD791Z MD791Z MDS20130171A MDS20130171A MD791Z MD 791 Z MD791 Z MD 791Z MD S20130171 A MDS20130171 A MD S20130171A MD S20130171 A MDS20130171 A MD S20130171A MD 791 Z MD791 Z MD 791Z
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- seeds
- common wheat
- wheat seeds
- viability
- treating common
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- 230000035899 viability Effects 0.000 title claims abstract description 14
- 238000000034 method Methods 0.000 title claims abstract description 13
- 244000098338 Triticum aestivum Species 0.000 title claims abstract description 11
- 239000007864 aqueous solution Substances 0.000 claims abstract description 6
- 229930002534 steroid glycoside Natural products 0.000 claims abstract description 5
- 238000002791 soaking Methods 0.000 claims abstract description 4
- 238000009331 sowing Methods 0.000 claims abstract description 4
- 241000208280 Hyoscyamus niger Species 0.000 abstract description 4
- 150000008143 steroidal glycosides Chemical class 0.000 abstract description 2
- 238000003808 methanol extraction Methods 0.000 abstract 1
- 230000035784 germination Effects 0.000 description 10
- 239000000243 solution Substances 0.000 description 9
- 238000002360 preparation method Methods 0.000 description 5
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 4
- 229930191978 Gibberellin Natural products 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 241000209140 Triticum Species 0.000 description 3
- 235000021307 Triticum Nutrition 0.000 description 3
- 239000003448 gibberellin Substances 0.000 description 3
- 230000007226 seed germination Effects 0.000 description 3
- 230000004936 stimulating effect Effects 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011066 ex-situ storage Methods 0.000 description 2
- 229930186308 hyoscyamoside Natural products 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 239000005980 Gibberellic acid Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 235000019714 Triticale Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- SIHHLZPXQLFPMC-UHFFFAOYSA-N chloroform;methanol;hydrate Chemical compound O.OC.ClC(Cl)Cl SIHHLZPXQLFPMC-UHFFFAOYSA-N 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 210000004020 intracellular membrane Anatomy 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000000401 methanolic extract Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 241000228158 x Triticosecale Species 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
Invenţia se referă la agricultură, şi anume la un procedeu de tratare a seminţelor de grâu comun cu viabilitate redusă. The invention relates to agriculture, namely to a process for treating common wheat seeds with reduced viability.
Păstrarea garantată a seminţelor plantelor cultivate în Băncile de Gene este legiferată prin standardele internaţionale care asigură un nivel înalt al viabilităţii lor în cadrul colecţiilor de bază şi celor active. De notat, însă, că la instituţiile de ameliorare şi cercetare mostrele de colecţie se conservă în colecţii de lucru în condiţii necontrolate de temperatură şi umiditate, ceea ce duce la scăderea viabilităţii lor după o perioadă scurtă de timp. În aceste împrejurări este necesar de a aplica diferite metode pentru mărirea germinaţiei lor. The guaranteed preservation of plant seeds cultivated in Gene Banks is legislated by international standards that ensure a high level of their viability within the basic and active collections. It should be noted, however, that at breeding and research institutions, collection samples are preserved in working collections under uncontrolled temperature and humidity conditions, which leads to a decrease in their viability after a short period of time. In these circumstances, it is necessary to apply various methods to increase their germination.
Este cunoscut faptul că pentru mărirea germinaţiei seminţelor de grâu păstrate în diferite condiţii se utilizează acidul giberelic AG3 [1]. It is known that gibberellic acid AG3 is used to increase the germination of wheat seeds stored under various conditions [1].
Neajunsurile procedeului menţionat constau în costul mare al preparatului, precum şi necesitatea utilizării alcoolului pentru prepararea soluţiilor. Plus la aceasta, folosirea giberelinei în scopuri practice este însoţită de un şir de manifestări nedorite: efectul variat asupra diferitor specii de plante cultivate şi în cadrul aceleiaşi specii, absenţa efectului stimulator la unele culturi sau inhibarea dezvoltării lor. The disadvantages of the mentioned procedure are the high cost of the preparation, as well as the need to use alcohol for the preparation of solutions. In addition, the use of gibberellin for practical purposes is accompanied by a number of undesirable manifestations: the varied effect on different species of cultivated plants and within the same species, the absence of a stimulating effect on some crops or inhibition of their development.
Cea mai apropiată soluţie este procedeul de tratare a seminţelor de triticale cu viabilitate redusă care include înmuierea acestora inainte de semănat cu soluţie apoasă de tomatozidă cu concentraţia de 0,005…0,05% timp de 18 ore [2]. The closest solution is the process of treating triticale seeds with reduced viability, which includes soaking them before sowing with an aqueous solution of tomatozide with a concentration of 0.005…0.05% for 18 hours [2].
Unul dintre neajunsurile procedeului dat poate fi considerat faptul că pentru obţinerea efectului stimulativ maximal privind mărirea viabilităţii seminţelor se cere utilizarea unor concentraţii mari ale tomatozidei. One of the shortcomings of this process can be considered the fact that to obtain the maximum stimulating effect on increasing seed viability, the use of high concentrations of tomatoside is required.
Problema pe care o rezolvă invenţia constă în sporirea viabilităţii seminţelor de grâu comun (Triticum aestivum L.) cu germinaţie redusă după păstrarea lor îndelungată în colecţiile de lucru şi cele active ex situ. The problem solved by the invention consists in increasing the viability of common wheat (Triticum aestivum L.) seeds with reduced germination after their long-term storage in working and active ex situ collections.
Problema se soluţionează prin aceea că procedeul de tratare a seminţelor de grâu comun cu viabilitate redusă propus include înmuierea acestora înainte de semănat într-o soluţie apoasă de glicozidă steroidică 3-O-{[β-D-glucopiranozil-(1→2)]-[β-D-glucopiranozil-(1→4)]-β-D-galactopiranozidă}-5α-furostan-3β,22α,26-triol (hiosciamozida F1) cu concentraţia de 0,0005…0,01%, timp de 24 ore. The problem is solved by the fact that the proposed process for treating common wheat seeds with reduced viability includes soaking them before sowing in an aqueous solution of the steroid glycoside 3-O-{[β-D-glucopyranosyl-(1→2)]-[β-D-glucopyranosyl-(1→4)]-β-D-galactopyranoside}-5α-furostane-3β,22α,26-triol (hyoscyamoside F1) with a concentration of 0.0005…0.01%, for 24 hours.
Rezultatul invenţiei constă în sporirea viabilităţii seminţelor de grâu comun după păstrarea lor îndelungată în colecţiile de lucru şi cele active ex situ. The result of the invention consists in increasing the viability of common wheat seeds after their long-term storage in working and active ex situ collections.
Utilizarea glicozidelor steroidice pentru prelucrarea seminţelor culturilor agricole este preferabilă, deoarece anterior s-a stabilit mecanismul acţiunii lor care constă în activizarea unor structuri membranare intracelulare, intensificarea proceselor metabolice, fapt ce micşorează acumularea radicalilor liberi, inhibă viteza de îmbătrânire a celulelor seminţelor. The use of steroid glycosides for processing agricultural crop seeds is preferable, because the mechanism of their action has previously been established, which consists in activating intracellular membrane structures, intensifying metabolic processes, which reduces the accumulation of free radicals, and inhibits the aging rate of seed cells.
Metoda de obţinere a hiosciamozidei F1 Method of obtaining hyosciamoside F1
Metoda de obţinere a preparatului constă în extragerea seminţelor degresate mărunţite de măsălăriţă neagră (Hyoscyamus niger L.) cu soluţie de 75% de metanol. Extractul metanolic se concentrează prin evaporare până la uscat şi multiplu se cromatografiază pe coloană cu silicagel. Controlul asupra randamentului preparatului se efectuează prin cromatografiere în straturi fine. Fracţiile, ce conţin preparatul, se unesc, se concentrează şi se obţine produsul scontat (temperatura de topire 183…184°C, [α]D 20 -65°) (H2O), Rf=0,35 (Cloroform-metanol-apă 65/35/30). (Nedova Irina. Structura glicozidelor steroidice din seminţe de Hyoscyamus niger L. şi activitatea lor biologică. Autoreferatul tezei de doctor în chimie. Chişinău, 2008, 26 p.). The method of obtaining the preparation consists in extracting the defatted crushed seeds of black henbane (Hyoscyamus niger L.) with a 75% methanol solution. The methanol extract is concentrated by evaporation to dryness and repeatedly chromatographed on a silica gel column. The control over the yield of the preparation is carried out by thin layer chromatography. The fractions containing the preparation are combined, concentrated and the expected product is obtained (melting point 183…184°C, [α]D 20 -65°) (H2O), Rf=0.35 (Chloroform-methanol-water 65/35/30). (Nedova Irina. Structure of steroidal glycosides from Hyoscyamus niger L. seeds and their biological activity. Abstract of the doctoral thesis in chemistry. Chisinau, 2008, 26 p.).
Exemplu de realizare a invenţiei Example of embodiment of the invention
Seminţele de grâu cu viabilitate redusă, soiul Dumbrăviţa (după păstrarea lor timp de 11 ani), se înmoaie în soluţii apoase de hiosciamozidă F1 în concentraţiile 0,0005…0,01% timp de 24 de ore la temperatura de 20ºC (invenţie). Pentru obţinerea datelor comparative seminţele se prelucrează cu soluţie apoasă (0,005%) de giberelină (analog) şi soluţie de tomatozidă (0,01%), (cea mai apropiată soluţie). Ca martor se utilizează seminţele umectate cu apă distilată. După 24 de ore seminţele se scot din soluţii, se spală şi se plasează în cutii Petri pe un strat de hârtie de filtru. Cutiile se introduc în termostat pentru germinare la temperatura de 20ºC. În fiecare variantă se utilizează câte 100 de seminţe în 4 repetări. Se determină energia germinativă (la a 4-a zi) şi germinaţia (la a 8-a zi). Rezultatele sunt prezentate în tabel. Wheat seeds with reduced viability, Dumbrăviţa variety (after being stored for 11 years), are soaked in aqueous solutions of hyoscyamoside F1 in concentrations of 0.0005…0.01% for 24 hours at a temperature of 20ºC (invention). To obtain comparative data, the seeds are processed with an aqueous solution (0.005%) of gibberellin (analog) and a solution of tomatoside (0.01%), (the closest solution). Seeds moistened with distilled water are used as a control. After 24 hours, the seeds are removed from the solutions, washed and placed in Petri dishes on a layer of filter paper. The dishes are placed in a thermostat for germination at a temperature of 20ºC. In each variant, 100 seeds are used in 4 repetitions. The germination energy (on the 4th day) and germination (on the 8th day) are determined. The results are presented in the table.
Tabel Table
Acţiunea hiosciamozidei F1 asupra seminţelor cu viabilitate redusă de grău comun The action of hyosciamoside F1 on seeds with reduced viability of common wheat
(soiul Dumbrăviţa) (Dumbrăviţa variety)
№ variantei Variantă Concentraţia, % Energia germinativă,% Germinaţia, % Lungimea rădăciniţelor, mm 1 Hiosciamozidă F1 0,0005 69,4±4,1 80,6±2,6 13,0±1,1* 2 “ - “ 0,001 86,3±5,1* 93,8±3,3* 14,2±1,6* 3 „ - „ 0,005 73,4±4,1* 84,4±2,9* 12,5±0,8* 4 „ - „ 0,01 65,3±3,8 75,0±2,1 11,5±1,2 5 „- „ 0,05 60,0±5,1 70,1±3,0 10,8±1,6 6 Giberelină 0,005 64,0±3,9 75,1±3,1 10,6±1,2 7 Tomatozidă 0,01 69,2±3,0 82,4±2,5* 11,1±1,4 8 Martor - 62,2±4,8 71,5±2,8 8,9±0,7Variant No. Variant Concentration, % Germination energy, % Germination, % Root length, mm 1 Hyosciamoside F1 0.0005 69.4±4.1 80.6±2.6 13.0±1.1* 2 “ - “ 0.001 86.3±5.1* 93.8±3.3* 14.2±1.6* 3 “ - “ 0.005 73.4±4.1* 84.4±2.9* 12.5±0.8* 4 “ - “ 0.01 65.3±3.8 75.0±2.1 11.5±1.2 5 “- “ 0.05 60.0±5.1 70.1±3.0 10.8±1.6 6 Gibberellin 0.005 64.0±3.9 75.1±3.1 10.6±1.2 7 Tomatoside 0.01 69.2±3.0 82.4±2.5* 11.1±1.4 8 Control - 62.2±4.8 71.5±2.8 8.9±0.7
• Diferenţe semnificative faţă de martor la P<0,05 • Significant differences compared to control at P<0.05
După cum reiese din tabelul prezentat, energia germinativă a seminţelor s-a majorat cu 24,1%, 22,3% şi 17,1%, comparativ cu martorul, analogul şi, respectiv, cea mai apropiată soluţie. Germinaţia totală a seminţelor s-a majorat corespunzător cu 22,3%, 18,7% şi 11,4%. Lungimea rădăcinuţelor în varianta respectivă a depăşit varianta martor cu 5,3 mm, analogul cu 3,6 mm şi cea mai apropiată soluţie cu 3,1 mm. De menţionat că efectul stimulativ maximal a fost obţinut în cazul utilizării soluţiei de 0,001% a hiosciamozidei F1. Concentraţia dată este de zece ori mai mică faţă de cea mai efectivă concentraţie a tomatozidei, cea ce este mai avantajos din punct de vedere economic. As can be seen from the table presented, the seed germination energy increased by 24.1%, 22.3% and 17.1%, compared to the control, the analogue and, respectively, the closest solution. The total seed germination increased accordingly by 22.3%, 18.7% and 11.4%. The length of the roots in the respective variant exceeded the control variant by 5.3 mm, the analogue by 3.6 mm and the closest solution by 3.1 mm. It should be noted that the maximum stimulating effect was obtained in the case of using the 0.001% solution of hyosciamoside F1. This concentration is ten times lower than the most effective concentration of tomatoside, which is more advantageous from an economic point of view.
Aşadar, tratarea prin procedeul propus a seminţelor de grâu comun cu viabilitate scăzută din cauza păstrării îndelungate contribuie la mărirea energiei lor germinative şi a germinaţiei totale. Therefore, the treatment of common wheat seeds with low viability due to long storage by the proposed process contributes to increasing their germination energy and total germination.
1. Ghobadi M., Abnavi M.S., Honarmand S. J. et al. Effect of hormonal priming (GA3) and osmopriming on behavoir of seed germination in wheat (Triticum aestivum L.). Journal of Agricultural Sciens, 2012, vol, 4, nr. 9, p. 244-250 1. Ghobadi M., Abnavi M.S., Honarmand S.J. et al. Effect of hormonal priming (GA3) and osmopriming on behavior of seed germination in wheat (Triticum aestivum L.). Journal of Agricultural Sciences, 2012, vol, 4, no. 9, p. 244-250
2. MD 154 Y 2010.03.31 2. MD 154 Y 2010.03.31
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| MDS20130171A MD791Z (en) | 2013-10-18 | 2013-10-18 | Process for treating common wheat seeds with low viability |
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| MD791Z true MD791Z (en) | 2015-02-28 |
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| MD1111Z (en) * | 2016-08-01 | 2017-08-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for treating common wheat seeds with reduced viability |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2069940C1 (en) * | 1992-07-17 | 1996-12-10 | Институт генетики АН Республика Молдова | Method of presowing wheat seeds treatment |
| MD2688G2 (en) * | 2004-07-02 | 2005-10-31 | Центр Растительных Генетических Ресурсов Молдовы Академии Наук Республики Молдова | Process for treating cucumber seeds with reduced viability |
| MD2716G2 (en) * | 2004-04-13 | 2005-10-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Antistressor for winter common wheat |
| MD2892G2 (en) * | 2005-04-06 | 2006-06-30 | Институт Физиологии Растений Академии Наук Республики Молдова | Trioside 3-O-{[b-D-glucopyranosyl(1 4)]-[b -D-glucopyranosyl(1 3)]- b-D-galactopyranoside}-(25R)-5a-furostan-3b,22a,26-triol-[26-O-b-D-glucopyranoside] - regulator of plant water exchange |
| MD3738G2 (en) * | 2008-05-21 | 2009-06-30 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Tetraoside of (25R)-5α-furostan-3β-22α-26-triol-[26-O-β-D-glucopyranoside] as compound increasing the seed distant hybridization setting |
| MD154Y (en) * | 2009-07-01 | 2010-03-31 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Process for treating triticale seeds with reduced viability |
| MD153Z (en) * | 2009-07-01 | 2010-10-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for treating chick pea seeds with reduced viability |
| EA201100449A1 (en) * | 2008-09-11 | 2011-10-31 | Басф Се | METHOD FOR INCREASING THE AMOUNT OF ENTITUTES FOR THE NUMBER OF SEEDS SEEDS |
| MD545Z (en) * | 2012-04-11 | 2013-05-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for treating tomato seeds with low viability |
-
2013
- 2013-10-18 MD MDS20130171A patent/MD791Z/en not_active IP Right Cessation
Patent Citations (9)
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| RU2069940C1 (en) * | 1992-07-17 | 1996-12-10 | Институт генетики АН Республика Молдова | Method of presowing wheat seeds treatment |
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| MD2688G2 (en) * | 2004-07-02 | 2005-10-31 | Центр Растительных Генетических Ресурсов Молдовы Академии Наук Республики Молдова | Process for treating cucumber seeds with reduced viability |
| MD2892G2 (en) * | 2005-04-06 | 2006-06-30 | Институт Физиологии Растений Академии Наук Республики Молдова | Trioside 3-O-{[b-D-glucopyranosyl(1 4)]-[b -D-glucopyranosyl(1 3)]- b-D-galactopyranoside}-(25R)-5a-furostan-3b,22a,26-triol-[26-O-b-D-glucopyranoside] - regulator of plant water exchange |
| MD3738G2 (en) * | 2008-05-21 | 2009-06-30 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Tetraoside of (25R)-5α-furostan-3β-22α-26-triol-[26-O-β-D-glucopyranoside] as compound increasing the seed distant hybridization setting |
| EA201100449A1 (en) * | 2008-09-11 | 2011-10-31 | Басф Се | METHOD FOR INCREASING THE AMOUNT OF ENTITUTES FOR THE NUMBER OF SEEDS SEEDS |
| MD154Y (en) * | 2009-07-01 | 2010-03-31 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Process for treating triticale seeds with reduced viability |
| MD153Z (en) * | 2009-07-01 | 2010-10-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for treating chick pea seeds with reduced viability |
| MD545Z (en) * | 2012-04-11 | 2013-05-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for treating tomato seeds with low viability |
Non-Patent Citations (1)
| Title |
|---|
| Ghobadi M., Abnavi M.S., Honarmand S. J. et al. Effect of hormonal priming (GA3) and osmopriming on behavoir of seed germination in wheat (Triticum aestivum L.). Journal of Agricultural Sciens, 2012, vol, 4, nr. 9, p. 244-250 * |
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| MD791Y (en) | 2014-07-31 |
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