MD1111Z - Process for treating common wheat seeds with reduced viability - Google Patents
Process for treating common wheat seeds with reduced viability Download PDFInfo
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- MD1111Z MD1111Z MDS20160093A MDS20160093A MD1111Z MD 1111 Z MD1111 Z MD 1111Z MD S20160093 A MDS20160093 A MD S20160093A MD S20160093 A MDS20160093 A MD S20160093A MD 1111 Z MD1111 Z MD 1111Z
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- Moldova
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- seeds
- common wheat
- wheat seeds
- reduced viability
- viability
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- 238000000034 method Methods 0.000 title claims abstract description 14
- 230000035899 viability Effects 0.000 title claims abstract description 13
- 244000098338 Triticum aestivum Species 0.000 title claims abstract description 12
- 239000000243 solution Substances 0.000 claims abstract description 9
- 239000007864 aqueous solution Substances 0.000 claims abstract description 7
- 241000196324 Embryophyta Species 0.000 claims abstract description 6
- 229930182470 glycoside Natural products 0.000 claims abstract description 5
- 150000002338 glycosides Chemical class 0.000 claims abstract description 5
- 241001530097 Verbascum Species 0.000 claims abstract description 4
- 238000013375 chromatographic separation Methods 0.000 claims abstract description 3
- 238000000605 extraction Methods 0.000 claims abstract description 3
- 238000002791 soaking Methods 0.000 claims abstract description 3
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 abstract 1
- QFRYQWYZSQDFOS-UHFFFAOYSA-N verbascoside Natural products CC1OC(COC2C(O)C(COC3OC(C(O)C(O)C3O)C(=O)O)OC(Oc4cc(O)cc5OC(=CC(=O)c45)c6ccc(O)c(O)c6)C2O)C(O)C(O)C1O QFRYQWYZSQDFOS-UHFFFAOYSA-N 0.000 description 10
- 230000035784 germination Effects 0.000 description 9
- KDSWDGKIENPKLB-QJDQKFITSA-N verbascoside Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](OC(=O)CCC=2C=C(O)C(O)=CC=2)[C@@H](CO)O[C@@H](OCCC=2C=C(O)C(O)=CC=2)[C@@H]1O KDSWDGKIENPKLB-QJDQKFITSA-N 0.000 description 9
- 230000004936 stimulating effect Effects 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 230000007774 longterm Effects 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000011066 ex-situ storage Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000007226 seed germination Effects 0.000 description 2
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 235000019714 Triticale Nutrition 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229930182486 flavonoid glycoside Natural products 0.000 description 1
- 150000007955 flavonoid glycosides Chemical class 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000005292 vacuum distillation Methods 0.000 description 1
- 241000228158 x Triticosecale Species 0.000 description 1
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- Saccharide Compounds (AREA)
Abstract
Изобретение относится к сельскому хозяйству, а именно к способу обработки семян мягкой пшеницы с пониженной жизнеспособностью.Способ, согласно изобретению, включает замачивание семян в течение 24 часов в 0,001…0,01% водном растворе дегидрокониферил-9-О-β-D-глюкопиранозида, полученного из растений Verbascum densiflorum Bertol. путем экстракции водноэтанольным раствором и хроматографического сепарирования гликозида с Rf 0,78.The invention relates to agriculture, and in particular to a method for treating soft wheat seeds with reduced viability. The method according to the invention includes soaking the seeds for 24 hours in a 0.001 ... 0.01% aqueous solution of dehydroconiferyl-9-O-β-D-glucopyranoside obtained from Verbascum densiflorum Bertol plants. by extraction with a water-ethanol solution and chromatographic separation of glycoside with Rf 0.78.
Description
Invenţia se referă la agricultură, şi anume la un procedeu de tratare a seminţelor de grâu comun cu viabilitate redusă după păstrarea lor îndelungată în colecţiile ex situ. The invention relates to agriculture, namely to a process for treating common wheat seeds with reduced viability after their long-term storage in ex situ collections.
Una din modalităţile de prezervare a resurselor genetice vegetale în Băncile de Gene ţine de păstrarea materialului semincer în condiţii controlate de mediu în corespundere cu standardele internaţionale care specifică procedurile de menţinere la nivelul corespunzător a viabilităţii seminţelor în colecţiile de bază sau cele active. De menţionat, însă, că în instituţiile de ameliorare şi cercetare din domeniile adiacente probele de seminţe ale genotipurilor incluse în studiu se păstrează, de regulă, în colecţii de lucru fără respectarea condiţiilor specifice de temperatură şi umiditate. Este evident că după o perioadă scurtă de timp se atestă diminuarea viabilităţii lor, ceea ce conduce la necesitatea aplicării unor metode de sporire a germinaţiei seminţelor. Importanţa efectuării acestor proceduri creşte exponenţial în cazul studiului unor forme valoroase pentru ameliorare sau conservare. One of the ways to preserve plant genetic resources in Gene Banks is to store seed material under controlled environmental conditions in accordance with international standards that specify procedures for maintaining seed viability at the appropriate level in basic or active collections. It should be noted, however, that in breeding and research institutions in adjacent fields, seed samples of the genotypes included in the study are usually kept in working collections without observing specific temperature and humidity conditions. It is obvious that after a short period of time, their viability decreases, which leads to the need to apply methods to increase seed germination. The importance of performing these procedures increases exponentially in the case of studying valuable forms for breeding or conservation.
Este cunoscut procedeul de tratare a seminţelor de plante cerealiere spicoase cu viabilitate redusă după păstrarea lor îndelungată cu soluţie apoasă de tomatozidă [1]. The process of treating seeds of spiked cereal plants with reduced viability after their long-term storage with an aqueous solution of tomatozide is known [1].
Unul din neajunsurile procedeului dat poate fi considerat faptul că pentru obţinerea efectului stimulativ maximal privind sporirea viabilităţii seminţelor de triticale este necesară o concentraţie sporită de tomatozidă. One of the shortcomings of this process can be considered the fact that to obtain the maximum stimulating effect on increasing the viability of triticale seeds, an increased concentration of tomatoside is required.
Problema pe care o rezolvă invenţia constă în mărirea viabilităţii seminţelor de grâu comun (Triticum aestivum L,) cu germinaţie redusă după păstrarea lor îndelungată ex situ în colecţiile de lucru. The problem solved by the invention consists in increasing the viability of common wheat seeds (Triticum aestivum L,) with reduced germination after their long-term storage ex situ in working collections.
Problema se soluţionează prin aceea că se propune un procedeu de tratare a seminţelor de grâu comun cu viabilitate redusă, care include înmuierea acestora timp de 24 ore într-o soluţie apoasă de 0,001…0,01% de dehidroconiferil-9-O-β-D-glucopiranozidă, obţinută din plante de Verbascum densiflorum Bertol. prin extragere cu soluţie hidroetanolică şi separare cromatografică a glicozidei cu un Rf de 0,78. The problem is solved by proposing a process for treating common wheat seeds with reduced viability, which includes soaking them for 24 hours in an aqueous solution of 0.001…0.01% dehydroconiferyl-9-O-β-D-glucopyranoside, obtained from Verbascum densiflorum Bertol plants, by extraction with hydroethanolic solution and chromatographic separation of the glycoside with an Rf of 0.78.
Rezultatul invenţiei constă în sporirea viabilităţii seminţelor de grâu comun după păstrarea lor îndelungată ex situ în colecţiile de lucru. The result of the invention consists in increasing the viability of common wheat seeds after their long-term ex situ storage in working collections.
Procesul de obţinere a glicozidei flavonoide menţionate (în continuare-verbascozida) constă în extragerea din plantele uscate de Verbascum densiflorum Bertol. cu etanol de 50% a complexului de glicozide, concentrarea extractului şi purificarea ulterioară a acestuia cu cloroform. Fracţiunea cu un conţinut predominant de glicozide cu valorile constantelor cromatografice Rf cuprinse între 0,83...0,75 a fost concentrată prin distilare în vid până la uscat şi separată prin cromatografiere pe silicagel şi sephadex G-50. Identificarea verbascozidei cu Rf 0,78 se efectuează pe plăci de silufol în sistemul de solvenţi cloroform:metanol:apă (65:30:5). The process of obtaining the mentioned flavonoid glycoside (hereinafter - verbascoside) consists of extracting from dried plants of Verbascum densiflorum Bertol. with 50% ethanol the glycoside complex, concentrating the extract and its subsequent purification with chloroform. The fraction with a predominant content of glycosides with the values of the chromatographic constants Rf ranging between 0.83...0.75 was concentrated by vacuum distillation to dryness and separated by chromatography on silica gel and sephadex G-50. The identification of verbascoside with Rf 0.78 is carried out on silufol plates in the solvent system chloroform:methanol:water (65:30:5).
Exemplul de realizare a invenţiei Embodiment of the invention
Seminţe îmbătrânite de grâu comun, soiul Moldova 93 (perioada de păstrare 7 ani), se înmoaie în soluţiile apoase de verbascozidă în concentraţii de 0,0001…0,1% timp de 24 ore la temperatura de 20°С (invenţia). Aged seeds of common wheat, variety Moldova 93 (storage period 7 years), are soaked in aqueous solutions of verbascoside in concentrations of 0.0001…0.1% for 24 hours at a temperature of 20°С (invention).
Pentru obţinerea unor rezultate comparative seminţele au fost prelucrate cu soluţie apoasă de tomatozidă în concentraţie de 0,01% timp de 24 ore (cea mai apropiată soluţie). Ca martor au servit seminţele umectate cu apă distilată (24 ore). După 24 ore de aplicare a soluţiilor, seminţele s-au clătit cu apă şi s-au amplasat în cutii Petri pe un substrat din hârtie de filtru umectată cu apă distilată. În fiecare variantă a experienţei s-au utilizat 4 cutii Petri cu câte 100 de seminţe. Cutiile s-au amplasat în termostat la temperatura de 20°С pentru verificarea germinaţiei seminţelor. Energia germinativă a fost determinată la a 4-a zi, iar germinaţia finală - la a 8-a zi. Rezultatele sunt prezentate în tabel. To obtain comparative results, the seeds were treated with an aqueous solution of tomatozide at a concentration of 0.01% for 24 hours (the closest solution). Seeds moistened with distilled water (24 hours) served as a control. After 24 hours of application of the solutions, the seeds were rinsed with water and placed in Petri dishes on a substrate of filter paper moistened with distilled water. In each variant of the experiment, 4 Petri dishes with 100 seeds each were used. The boxes were placed in a thermostat at a temperature of 20°С to check seed germination. Germination energy was determined on the 4th day, and final germination - on the 8th day. The results are presented in the table.
Tabel Table
Acţiunea verbascozidei asupra energiei de creştere şi a germinaţiei seminţelor de grâu comun, soiul Moldova 93 (reproducţia anului 2009) The action of verbascoside on the growth energy and germination of common wheat seeds, Moldova 93 variety (2009 reproduction)
№ variantei Varianta Concentraţia, % Energia germinativă, % Germinaţia, % Lungimea rădăciniţelor, mm 1 Verbascozidă 0,1 42,0±2,6 58,0±3,8 12,0±1,1 2 “ - “ 0,01 57,4±2,2* 68,6±3,9* 14,4±1,2* 3 “ - “ 0,001 60,0±2,8* 90,4±1,5* 14,6±1,6* 4 „ - „ 0,0001 51,4±2,6* 66,6±3,1* 13,8±1,7* 5 Tomatozidă 0,01 45,0±1,6 62,0±2,4 12,5±1,3 6 Martor - 40,0±2,4 54,0±4,2 10,3±1,4Variant No. Variant Concentration, % Germination energy, % Germination, % Root length, mm 1 Verbascoside 0.1 42.0±2.6 58.0±3.8 12.0±1.1 2 “ - “ 0.01 57.4±2.2* 68.6±3.9* 14.4±1.2* 3 “ - “ 0.001 60.0±2.8* 90.4±1.5* 14.6±1.6* 4 “ - “ 0.0001 51.4±2.6* 66.6±3.1* 13.8±1.7* 5 Tomatoside 0.01 45.0±1.6 62.0±2.4 12.5±1.3 6 Control - 40.0±2.4 54.0±4.2 10.3±1.4
* - Diferenţe semnificative faţă de martor la P<0,05 * - Significant differences compared to control at P<0.05
După cum rezultă din tabelul prezentat, acţiune stimulatoare s-a observat la utilizarea concentraţiilor de 0,0001%…0,01% ale verbascozidei. Efectul stimulator maxim s-a depistat la utilizarea concentraţiei de 0,001% a verbascozidei. Energia germinativă a seminţelor s-a majorat comparativ cu martorul cu 20,0%, iar cu cea mai apropiată soluţie - cu 15,0%. Germinaţia finală a seminţelor de grâu comun a fost superioară martorului cu 36,4% şi cu cea mai apropiată soluţie - cu 28,4%. Lungimea rădăciniţelor în varianta stimulatoare (concentraţia verbascozidei de 0,001%) faţă de martor a fost mai mare cu 4,3 mm şi cu 2,1 mm comparativ cu cea mai apropiată soluţie. As can be seen from the table presented, stimulating action was observed when using concentrations of 0.0001%…0.01% of verbascoside. The maximum stimulating effect was detected when using a concentration of 0.001% of verbascoside. The germination energy of the seeds increased compared to the control by 20.0%, and with the closest solution - by 15.0%. The final germination of common wheat seeds was superior to the control by 36.4% and with the closest solution - by 28.4%. The length of the roots in the stimulating variant (verbascoside concentration of 0.001%) compared to the control was greater by 4.3 mm and by 2.1 mm compared to the closest solution.
De notat că concentraţia stimulativă maximă a verbascozidei depistată este de zece ori mai mică decât la cea mai apropiată soluţie (tomatozida), ceea ce este mai avantajos din punct de vedere al eficienţei şi rentabilităţii procedeului. It should be noted that the maximum stimulating concentration of verbascoside detected is ten times lower than the closest solution (tomatoside), which is more advantageous from the point of view of the efficiency and profitability of the process.
Astfel, procedeul de tratare înainte de semănat a seminţelor îmbătrânite de grâu comun cu viabilitatea redusă cu soluţii apoase de verbascozidă în concentraţii de 0,0001…0,01% contribuie la majorarea energiei germinative şi a germinaţiei seminţelor, mărindu-se astfel viabilitatea lor. Thus, the pre-sowing treatment process of aged common wheat seeds with reduced viability with aqueous solutions of verbascoside in concentrations of 0.0001…0.01% contributes to increasing the germinative energy and germination of seeds, thus increasing their viability.
1. MD 154 Y 2010.03.31 1. MD 154 Y 2010.03.31
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| MDS20160093A MD1111Z (en) | 2016-08-01 | 2016-08-01 | Process for treating common wheat seeds with reduced viability |
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| MDS20160093A MD1111Z (en) | 2016-08-01 | 2016-08-01 | Process for treating common wheat seeds with reduced viability |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD154Y (en) * | 2009-07-01 | 2010-03-31 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Process for treating triticale seeds with reduced viability |
| MD791Y (en) * | 2013-10-18 | 2014-07-31 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | Process for treating common wheat seeds with low viability |
| MD901Y (en) * | 2014-10-31 | 2015-05-31 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | Process for presowing treatment of cucumber seeds |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD154Y (en) * | 2009-07-01 | 2010-03-31 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Process for treating triticale seeds with reduced viability |
| MD791Y (en) * | 2013-10-18 | 2014-07-31 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | Process for treating common wheat seeds with low viability |
| MD901Y (en) * | 2014-10-31 | 2015-05-31 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | Process for presowing treatment of cucumber seeds |
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