KR101729210B1 - Development of antiatopic dermatitis targeted products using Abeliophyllum distichum Nakai - Google Patents

Abstract

The present invention relates to a composition containing an extract of Helicobacter pylori extract for improving an inflammatory skin disease (atopic dermatitis) using an extract of Helicobacter pylori extract having an anti-inflammatory effect extracted from Abeniophyllum distichum, It is possible to prevent disease by introducing a composition containing an extract of Helicobidae extracted with ethanol as a solvent as an effective ingredient and evolving from the concept of simple beauty to introduce the concepts of aging and disease prevention and treatment .

Description

[0001] The present invention relates to an anti-atopic dermatitis targeted product using Abelophyllum distichum Nakai,

The present invention relates to a composition for improving atopic dermatitis comprising a herbaceous plant extract, and more particularly, to a composition for improving atopic dermatitis by using an extract of Helicobacter pylori extract having anti-inflammatory activity extracted from Abeniophyllum distichum, And to a composition containing an extract of Helicobida var.

Inflammation is a localized immune response when tissues are damaged or destroyed by harmful substances or organisms from the outside, which is a useful defense mechanism to protect living organisms and remove the products of tissue damage. In normal cases, the living body can neutralize or eliminate the causative factors through the inflammatory reaction, regenerate the upper tissue, restore normal structure and function, but also progress to a disease state such as chronic inflammation. It is known that not only inflammatory reaction can be observed in almost all diseases among clinical diseases but also enzymes related to inflammation reaction play an important role in carcinogenesis.

The anti-inflammatory effects of most nonsteroidal antiinflammatory drugs (NSAIDs) are mediated by inhibiting cyclooxygenase (COX) enzyme activity. COX is the main enzyme involved in the biosynthesis of prostaglandin in vivo. There are two kinds of isozymes, COX-1 and COX-2. COX-1 is constantly present in tissues such as the stomach or kidney and is involved in maintaining normal homeostasis, whereas COX-2 is involved in inflammatory and other immune responses by mitogen or cytokines Which is a transient and rapidly expressed enzyme. Non-steroidal anti-inflammatory drugs used in the treatment of chronic inflammatory diseases such as acute or rheumatoid arthritis are known to exhibit side effects such as gastrointestinal disorders by inhibiting COX-1 enzyme as well as inhibiting COX-2 enzyme. Therefore, in order to increase the efficacy of the anti-inflammatory drug and minimize the side effects, it is necessary to find a substance that selectively inhibits COX-2 enzyme and develop it as anti-inflammatory drug.

In recent years, the incidence of atopy has increased due to external factors such as atmospheric pollution, environmental hormones and water pollution, and excessive stress. However, the causes and mechanism of action have been various, and DSCG (disodium cromoglycate), tranilant , ketotifen, azelastine, etc., have been increasing their interest and research for substitution with plant extracts because of problems such as side effects.

In other words, corticosteroids and antihistamines have been used as therapeutic agents for inflammatory skin diseases. However, various side effects have been reported when the therapeutic agents are administered for a long period of time. Therefore, there is a need to develop new therapeutic agents. It is preferentially selected.

Examples of such techniques are described in documents 1 and 2 below.

For example, the following Patent Document 1 discloses a method of preparing an extract having histamine inhibitory effect by extracting several kinds of solvents on a spruce tree, measuring the antioxidant effect of the extract, and measuring histamine-releasing activity by measuring β-Hexosaminidase releasing assay using RBL-2H3 Ratbasophil cell Inhibiting effect and the resulting atopic dermatitis effect, and also to form an atopic symptom-reducing cosmetic composition containing the extract, thereby alleviating atopic symptoms, and a cosmetic composition containing the same.

In addition, Patent Document 2 discloses a method of finely grinding and drying fine linen that has been washed and dried, then finely grinding it using a 300 mesh sheave, adding 50 mL of rice bran and soybean fermentation agent at a ratio of 50 g / L, and sterilizing at 120 ° C., Aspergillus oryzae was first fermented for 1 ~ 3 days at 25 ~ 38 ℃, and 1L of purified water was poured into it. After immersion, the resultant was immersed and the resultant mixture of lactic acid bacteria, Leuconostoc citreum, Lactobacillus sakei and yeast Saccharomyces carlsbergensis were inoculated, and the fermentation process was anaerobically and aerobically performed at 10 to 38 ° C for 1 to 4 days, Discloses a composition for cosmetics comprising a fermented product, followed by centrifuging and removing the fermenting bacteria first, followed by filtration.

Korean Registered Patent No. 10-1191225 (registered on October 10, 2012) Korean Patent Laid-Open Publication No. 2013-0074172 (published on July 04, 2013)

However, conventional techniques such as those described above have lacked systematic and scientific evidence and clinical results.

DISCLOSURE Technical Problem The present invention has been made in order to solve the above-mentioned problems, and it is an object of the present invention to provide an anti-inflammatory activity and to inhibit NO, iNOS, COX-2 and IL- And suppresses the activation of ERK1 / 2 among the MAPK signaling components and inhibits the accumulation of cytoplasmic and nuclear β-catenin, as a cosmetic composition for improving atopic dermatitis, an inflammatory skin disease .

Another object of the present invention is to provide a cosmetic composition for improving inflammatory skin diseases according to scientific evidence and clinical results.

In order to achieve the above object, the cosmetic composition for improving atopic dermatitis according to the present invention is characterized in that it contains an extract of Abelliophyllum distichum extracted with ethanol as an effective solvent.

In addition, the cosmetic composition for improving atopic dermatitis which is an inflammatory skin disease according to the present invention is characterized in that the extraction solvent is 100% ethanol.

In addition, the cosmetic composition for improving atopic dermatitis, an inflammatory skin disease according to the present invention, may further comprise at least one member selected from the group consisting of leaf, stem, bark, roots, seeds and flowers.

In the cosmetic composition for improving atopic dermatitis, an inflammatory skin disease according to the present invention, the composition is prepared by immersing 100% ethanol as a leaf and extracting solvent for 3 days in an extractive or reflux extractor at 100 DEG C for 4 hours Filtrated through a filter paper, concentrated under reduced pressure and lyophilized to obtain powder.

In the cosmetic composition for improving atopic dermatitis according to the present invention, the composition may be at least one selected from the group consisting of a paste, a gel, a cream, a lotion, a powder, a solid soap, a water soap, a shampoo, a rinse, a solution, a suspension, , Powder perfume, surfactant-containing cleansing or surfactant-free cleansing, oil, powder foundation, emulsion foundation, hair dye, wax, suppository lotion, nutritional lotion, nutritional cream, massage cream, essence, cleansing cream, cleansing foam, pack , A pack base, an eye cream, a perfume, an ointment, a cleansing water, a powder, a wax foundation, and a spray.

As described above, according to the cosmetic composition for improving atopic dermatitis, an inflammatory skin disease according to the present invention, systematic and scientific analysis of efficacy can be used to identify new functionalities and develop materials related to health functional products in the future Industrial use of materials through various biological tests Establishment of value and improvement of reliability of material through scientific proof of improvement effect of atopic dermatitis Since beauty products evolve from simple cosmetic concept and the concepts of aging and disease prevention and treatment are introduced, Can be made possible.

Further, according to the cosmetic composition for improving atopic dermatitis which is an inflammatory skin disease according to the present invention, it is possible to obtain an effect of contributing to the high value added of beech tree and the increase of income of the beech tree farmer.

Figure 1 is a photograph showing the effect of Leucillus leaf extract on NO production and iNOS inhibition in LPS-stimulated RAW 264.7 cells,
FIG. 2 is a photograph showing the effect of Leucnet leaf extract on chronic inflammation inducers in LPS-stimulated RAW 264.7 cells,
FIG. 3 is a photograph showing the effect of Leucillife leaf extract on NF-kB activation,
Fig. 4 is a photograph showing the effect of Leucillife leaf extract on NF-kB luciferase activation,
FIG. 5 is a photograph showing the effect of Leucillus leaf extract on ERK1 / 2 activation,
FIG. 6 is a photograph showing the effect of Leucillus leaf extract on β-catenin activation,
FIG. 7 is a photograph showing the effect of Leucillus leaf extract on the expression of Nox.

These and other objects and novel features of the present invention will become more apparent from the description of the present specification and the accompanying drawings.

The cosmetic composition for improving atopic dermatitis, an inflammatory skin disease according to the present invention, contains an extract of Abelliophyllum distichum extracted with 100% ethanol as an extraction solvent as an active ingredient.

Abeliophyllum distichum applied to the present invention is a deciduous shrub of the Asiatic tree ash tree and is a native plant of Korea designated as a Natural Monument No. 364 and is native to Goesan, Chungbuk, Buan, Jeonbuk. It is a basic species in which white flowers bloom.

It is said that the pink flower is bloomed for.lilacinum, the ivory flower is for the eburneum, the calyx is light green for the viridicalycinum, (var. rotundicarpum), and spikelets usually form a cluster and grow wild.

It is a special species of our country which is only one species in the world. The name of a rare species is the shape of fruit is a type of round fan used by ancient ancestors (a fan of the king) It is a name attached because it looks like a tail.

It grows at the base of a sunny mountain with a hardness of 1m, the end of the branch is sagging, the purple color is rounded, and the young branch is angled. Leaves are opposite to each other and arranged in 2 lines, egg-shaped or oval-shaped, egg-shaped, 3-8cm long, 5-30mm wide, pointed end, rounded bottom, flat edge. The petiole is 2 ~ 5mm in length.

The flower is the flower of the forsythia flower before the leaf, and the flower of the flower is the flower of the gun. There are also pink flowers that are not common. Flowers of the fine line tree are excellent in fragrance, calyx is bell-shaped square, length is 3 ~ 3.5mm and divided into 4 pieces, and the cracked piece is egg-shaped or egg-shaped circle. Corolla is longer than calyx, is divided into 4 pieces, and there are 2 stamens. The fruit is oval, round oval, 25mm long, concave at the end, wing around, and contains 2 seeds. They breed with seeds and bones. The geographical feature of the native trees is a unique ecology that grows in roughly stone fields.

The term "spruce tree extract" as used in the present invention refers to a plant obtained by extracting from various organs or parts of the brackish tree such as leaves, flowers, roots, stems, husks and seeds, Powdered into a size of 20 to 500 mesh and extracted.

The herbal extract of the present invention can be prepared using conventional solvents under the conditions of ordinary temperature and pressure, and preferably purified water or ethanol can be subjected to reflux extraction using an extraction solvent.

In addition, the microcrystal extract of the present invention includes not only the extract obtained by the above-mentioned extraction solvent, but also the extract obtained through a conventional purification process. For example, decompression by carbon dioxide, extraction by supercritical extraction at high temperature, extraction by extraction using ultrasound, separation by ultrafiltration membrane with constant molecular weight cutoff value, separation of various chromatographies (size, charge, hydrophobicity or affinity And the active fraction obtained through various purification methods such as separation by means of separation according to sex) are also included in the extract of the present invention.

EXAMPLES 1-4: Extraction of Composition from Leafy Leaves.

(Example 1), 50% ethanol (Example 2), 70% ethanol (Example 3) and 100% ethanol (Example 4) were added to 50 g of each sample, 300 ml each of them is added, and the mixture is stirred for 3 days and then dipped and extracted.

(Example 1), 50% ethanol (Example 2), 70% ethanol (Example 3) and 100% ethanol (Example 4) were added to 50 g of each sample, 300 ml was added to the mixture, and the mixture was extracted with a reflux condenser at 100 ° C for 4 hours.

Each of the extracts according to Examples 1 to 4 was filtered with a filter paper (No. 2), and the extract was powdered by concentration under reduced pressure and lyophilized to be used as a sample for the anti-inflammatory effect test.

Using the above samples, anti-inflammatory activity of Leucocarpus leaf extract was carried out.

Experimental Example 1: Inhibition of nitric oxide (NO) production

The amount of nitric oxide (NO) produced from the RAW 264.7 cell line was measured using Griess reagent as the form of NO ₂ ^- present in the cell culture.

That is, RAW 264.7 cells were seeded on a 24-well plate at 5 × 10 ⁵ / well and cultured for 12 hours.

Leaf extracts of Echinochloa crus-galli were treated with LPS (lipopolysaccharide) at a concentration of 1 ㎍ / ㎖ for 2 hours and then cultured for 24 hours.

After 24 hours, 200 μl of the cell culture supernatant was mixed with 800 μl of Griess reagent [0.1% N- (1-napthyl) -ethylenediamine, 1% sulfanilamide in 5% phosphoric acid] and reacted at room temperature for 10 minutes. Respectively.

Experimental Example 2: Inhibition of Chronic Inflammatory Inducing Factor

RAW 264.7 cells were plated on a 6-well plate at 2 × 10 ⁶ / well and cultured for 12 hours.

Leaf leaf extracts were treated with LPS at a concentration of 1 μg / ㎖ for 2 hours and cultured for 18 hours.

The mRNA was isolated from the cells using the RNeasy kit, cDNA was synthesized using the Verso cDNA kit, and the target gene was amplified using a primer and a PCR master mix of chronic inflammation inducers.

After the amplification, the inhibition of the production of chronic inflammation inducing factors by the leaf extract was confirmed by the increase and decrease of the expression band by DNA electrophoresis.

Experimental Example 3: Regulation of NF-kB signaling of Leaf leaf extract

First, IkB-α decomposition inhibitory effect of leaf extract of Pinus rigida was examined.

RAW 264.7 cells are seeded in a 6-well plate at 2 × 10 ⁶ / well and cultured for 12 hours.

Leaf leaf extracts were treated with LPS at a concentration of 1 μl / ml for 2 hours, followed by incubation for 15 minutes.

After culturing, proteins were extracted from the cells, and the level of IkB-α protein was confirmed by SDS-PAGE and western blotting.

In addition, the effect of inhibiting p65 nuclear translocation of Leaf Extract of Myxobacteria was examined.

RAW 264.7 cells are seeded in a 6-well plate at 2 × 10 ⁶ / well and cultured for 12 hours.

Leaf extract of Echinochloa crus-galli is treated at a concentration of 1 μl / ml with LPS for 2 hours and cultured for 30 minutes.

Quantification of proteins by BCA method after cytosol and nucleus separation by using Nucleus extract kit from cells after culturing. Cytoplasmic and nuclear p65 protein levels were confirmed by SDS-PAGE and western blotting.

In addition, the inhibitory activity of NF-kB transcriptional activity on the extracts of Leuconostoc sp.

RAW 264.7 cells were plated on a 12-well plate at 110 ⁵ / well and cultured for 12 hours.

After incubation, transfection of NF-kB luciferase plasmid was performed for 24 hours using polyJet DNA transfection reagent. Leuconostoc spider leaf extracts were treated with LPS (lipopolysaccharide) at a concentration of 1 g / ml for 2 hours and cultured for 18 hours. After incubation, NF-kB luciferase was measured using a luminometer.

Experimental Example 4: Regulation of MAPK signaling of Leaf leaf extract

RAW 264.7 cells were plated on a 6-well plate at 2 × 10 ⁶ / well and cultured for 12 hours. Leaf leaf extracts were treated at a concentration of 1 μl / ml of LPS for 2 hours, followed by incubation for 15 minutes.

After culturing, the proteins were extracted from the cells, and p-ERK1 / 2 and total ERK1 / 2 protein levels were confirmed by SDS-PAGE and western blotting.

Experimental Example 5: Regulation of β-catenin signaling of leaf extract

The inhibitory effect of β-catenin accumulation on the leaf extract was investigated.

RAW 264.7 cells were plated on a 6-well plate at a density of 2 × 10 ⁶ / well and cultured for 12 hours. The extracts were treated with LPS at a concentration of 1 μl / ml for 1 hour.

Quantification of proteins by BCA method after cytosol and nucleus separation by using Nucleus extract kit from cells after culturing. The cytoplasm and nuclear β-catenin protein levels were confirmed by SDS-PAGE and western blotting.

In addition, the effect of inhibiting the expression of NADPH oxidase (Nox) in Leuconostoc spider leaf extract was examined.

RAW 264.7 cells were seeded on a 6-well plate at 210 ⁶ / well and incubated for 12 hours. The leaf extracts were treated with LPS at a concentration of 1 g / ml for 2 hours and cultured for 18 hours. After mRNA was isolated from cells using RNeasy kit, cDNA was synthesized using Verso cDNA kit and target gene was amplified using Nox primer and PCR master mix. After the amplification, the inhibition of Nox formation by the extracts of Leucumber leaf was confirmed by the increase and decrease of the expression band by DNA electrophoresis.

The results of the anti-inflammatory activity tests of the leaf extracts of the above-mentioned trees were as follows.

The inflammatory reaction is caused by a local defense reaction of biotissue against the invasion of bacteria or foreign matter, and at the same time, macrophages are activated by inflammatory cells and defense mechanisms.

The macrophages activated by external stimuli are mediated through various signal pathways such as inflammatory mediators (iNOS, COX-2), cytokines (TNF-α, IL-1β, IL-6), histamine, PGE ₂ , complement and chemokine .

In general, NO, PGE ₂ , TNF-α, IL-1β and IL-6, which are normally produced under normal conditions, are involved in the regulation of normal physiological functions, but when overproduced by stimulation, diabetes, arteriosclerosis, Alzheimer's disease, Post-infectious diseases such as reperfusion injury, cancer and post-infectious diseases such as meningitis and rheumatic fever, infiltrative lupus erythematosus and rheumatoid arthritis, and various other inflammatory diseases.

Figure 1 is a photograph showing the effect of Leucillus leaf extract on NO production and iNOS inhibition in LPS-stimulated RAW 264.7 cells.

As shown in FIG. 1 (A), it was found that the inhibitory activity of NO production on the part of the spike, that is, flowers, leaves and branches, showed the highest inhibitory activity in the leaf extract of Pinus rigida.

As shown in FIG. 1 (B), the higher the concentration of ethanol as the extraction solvent, the higher the inhibitory activity of NO production, according to the extraction solvents according to Examples 1 to 4. And it was found.

That is, as shown in FIG. 1 (C), the inhibitory activity of NO production by the concentration of Leuconostoc spider leaf extracted with 100% ethanol was evaluated, and it was found that the inhibitory activity against NO production was shown in a concentration dependent manner.

In addition, as shown in FIG. 1 (D), the extract of Helicobacter pylori extracted with 100% ethanol inhibits the expression of iNOS (nitric oxide synthases), an inflammatory mediator closely related to NO production Could know. These results suggest that Leuconthus sp. Leaf extract inhibits NO production by regulating iNOS expression.

As shown in FIG. 2, the extracts of Leuconostoc sp. Leaves extracted with 100% ethanol inhibited the expression of COX-2 and IL-1β, which are chronic inflammation inducers, in a concentration-dependent manner. Lt; RTI ID = 0.0 > MMP-9. ≪ / RTI >

Fig. 2 is a photograph showing the effect of Leucillife leaf extract on chronic inflammation inducers in LPS-stimulated RAW 264.7 cells.

Next, the regulation of NF-kB signaling of the leaf extract of the brackish tree according to the present invention will be described.

Expression of chronic inflammatory agents in inflammatory responses is regulated by NF-kB signaling. Thus, NF-kB signaling is considered an important molecular target for chronic inflammation control.

NF-kB is composed of a homodimer or heterodimer of the p50 subunit family (p50, p52) and the p65 subunit family (p65, c-Rel, RelB) ) I _K B protein (I _K Bα, I _K Bβ, I _K Bγ, I _K Bε, and Bcl3) and is present in the cytoplasm in an activated state.

However, I _K B protein is phosphorylated by various stimuli such as cytokines (TNF-α, IL-1), bacterial / viral infections (LPS, dsRNA), stress (ROI, UV, adriamycin, radiation) NF- _K B liberated by decomposition enters the nucleus and binds to the NF- _K B binding site of the target gene, thereby inducing the expression of an inflammation-related gene and an anti-apoptosis gene.

Phosphorylation of I _K B protein is a I _K B, known as active enzyme IKK (IKKα, β) that phosphorylate serine residue (serine residue) of I _K B protein is activated by a variety of stimuli and, phosphorylation of I _K B protein 26S It is degraded by proteasome. The transcription factor NF- _K B is activated by proinflammatory cytokines such as TNF-α, IL-1β and LPS, and induces tissue damage by accelerating the inflammatory reaction by increasing the expression of inflammatory enzymes iNOS and COX-2. Thus, effective inhibition of NF-kB signaling plays an important role in the prevention of inflammation.

As can be seen from FIG. 3 (A), the extract of Leucumber Leaf Extract using 100% Ethanol according to Example 4 of the present invention shows the decomposition of I _K Ba necessary for the nuclear transfer of NF-kB in the inflammatory reaction As shown in FIG. 3 (B), it was found that the inhibition of p65 nuclear transfer was inhibited.

FIG. 3 is a photograph showing the effect of Leucon leaf extract on NF-kB activation.

The inhibition of p65 nuclear transfer on the NF-kB transcriptional activity of Leuconostoc sp. Leaf extracts extracted with 100% ethanol resulted in the inhibition of NF-kB transcription by LPS, an inflammation mediator, kB transcriptional activity was increased. However, as shown in FIG. 4, the transcriptional activity of NF-kB by LPS was inhibited in the cells treated with Echinosophora koreensis extract. Thus, the results of the present invention indicate that Leuconthus leaf extract inhibits the degradation of IB-a induced by LPS to inhibit the nuclear translocation of p65 and minimize the transcriptional activity of NF-kB, thereby inducing an anti-inflammatory response .

FIG. 4 is a photograph showing the effect of Leucon leaf extract on NF-kB luciferase activation.

Next, Describe the regulation of ERK1 / 2 signaling in Leucocephala leaf extract.

Mitogen-activated protein kinase (MAPK) plays an important role in regulating cell growth and differentiation, and regulating cellular responses to cytokines and stress. The MAPK signaling pathway has been evolutionarily conserved and plays an important role in transferring information from the cell environment to the nucleus through the cytoplasm, with at least three signaling pathways.

Activated ERK in extracellular signal-regulated kinase (ERK) signaling pathways can phosphorylate various transcription factors. While ERK is extensively activated by stimulatory factors, p38 and JNK constitute part of the stress response pathway and are activated by cellular stress induced by factors such as pro-inflammatory cytokines. JNK signaling pathways are activated in cells that respond to immunostimulatory stimuli and are involved in morphological enhancement of cells and cytokine transcription.

External harmful factors stimulate TLR4 on the surface of macrophages to induce activation of MAPK, a subcellular signaling pathway. Activated MAPK signaling pathways are involved in the expression of various inflammatory mediators such as pro-inflammatory cytokines, NO, and PGE ₂ .

As shown in FIG. 5, the effect of LPS-induced ERK1 on the MAPK signal transduction pathway in the present invention was examined using 100% ethanol according to Example 4, / 2 < / RTI >

FIG. 5 is a photograph showing the effect of Leucilliform Leaf Extract on ERK1 / 2 activation. FIG.

These results suggest that Leuconostoc spider leaf extract exerts anti - inflammatory activity through inhibition of MAPK signaling pathway related to ERK1 / 2 activation.

Next, the β-catenin signaling regulation of the Leucocephala leaf extract according to the present invention is described.

Macrophages responsible for innate immune responses secrete inflammatory mediators as well as phagocytosis of external harmful factors. Reactive oxygen species in inflammatory mediators of macrophages are originally human defense systems against external harmful factors, but excessive production of active oxygen causes or exacerbates inflammatory dermatitis such as atopy.

The external harmful factor induces excessive phosphorylation of macrophage GSK3 to induce inactivation, leading to an increase in the level of β-catenin in the cytoplasm, and the accumulation of β-catenin in the cytoplasm is transferred to the nucleus, Induced NADPH oxidase (Nox) expression, leading to overexpression of reactive oxygen species.

Therefore, the relationship between active oxygen and inflammatory dermatitis such as atopy, and the relationship between β-catenin and reactive oxygen species, inhibits activation of β-catenin signaling by external harmful factors is important for improving inflammatory dermatitis such as atopy It is considered a molecular target.

As a result of evaluating the effect of β-catenin signaling on the β-catenin signal transduction according to the present invention, inhibition of β-catenin by nuclear transfer showed that the Leucon leaf extract extracted with 100% ethanol according to Example 4, , It was found that the accumulation of β-catenin in the cytoplasm and nucleus was inhibited by the external harmful factor LPS.

Fig. 6 is a photograph showing the effect of Leucilliform Leaf Extract on β-catenin activation.

As shown in FIG. 7, the expression of Nox was increased in cells not treated with the extract of Leuconostoc mesentericum. However, as shown in FIG. 7, The expression of Nox was inhibited in a concentration - dependent manner in the cells treated with the leaf - leaf extract.

FIG. 7 is a photograph showing the effect of Leucillife leaf extract on the expression of Nox.

Although the present invention has been described in detail with reference to the above embodiments, it is needless to say that the present invention is not limited to the above-described embodiments, and various modifications may be made without departing from the spirit of the present invention.

By using the cosmetic composition according to the present invention, atopic dermatitis which is an inflammatory skin disease can be improved.

Claims (5)

delete delete delete A cosmetic composition for improving atopic dermatitis which is an inflammatory skin disease containing an extract of Abelliophyllum distichum extracted with ethanol as an extraction solvent as an active ingredient,
The cosmetic composition was prepared by dipping in 100% ethanol for 3 days as a leaf and extracting solvent for 3 days, extracting it at 100 ° C for 4 hours in an extraction or reflux extractor, filtering through a filter paper, and concentrating under reduced pressure and freeze- Wherein the atopic dermatitis is an inflammatory skin disease. 5. The method of claim 4,
The composition may be in the form of a paste, gel, cream, lotion, powder, solid soap, water soap, shampoo, rinse, solution, suspension, emulsion, mineral cosmetic, powder perfume, surfactant- Cream, essence, cleansing cream, cleansing foam, pack, pack base, eye cream, perfume, ointment, cleansing water, powder, wax foundation, foundation, emulsion foundation, hair dye, wax, Wherein the atorvastatin is at least one selected from the group consisting of atorvastatin and atorvastatin.

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