MD901Z - Process for presowing treatment of cucumber seeds - Google Patents
Process for presowing treatment of cucumber seeds Download PDFInfo
- Publication number
- MD901Z MD901Z MDS20140135A MDS20140135A MD901Z MD 901 Z MD901 Z MD 901Z MD S20140135 A MDS20140135 A MD S20140135A MD S20140135 A MDS20140135 A MD S20140135A MD 901 Z MD901 Z MD 901Z
- Authority
- MD
- Moldova
- Prior art keywords
- cucumber seeds
- solution
- verbascoside
- cucumber
- seeds
- Prior art date
Links
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 11
- 244000299906 Cucumis sativus var. sativus Species 0.000 title claims 2
- 239000000243 solution Substances 0.000 claims abstract description 9
- 241000196324 Embryophyta Species 0.000 claims abstract description 5
- 239000007864 aqueous solution Substances 0.000 claims abstract description 5
- 238000009331 sowing Methods 0.000 claims abstract description 5
- 241001530097 Verbascum Species 0.000 claims abstract description 4
- 238000002791 soaking Methods 0.000 claims abstract description 4
- 238000013375 chromatographic separation Methods 0.000 claims abstract description 3
- 238000000605 extraction Methods 0.000 claims abstract description 3
- 240000008067 Cucumis sativus Species 0.000 abstract description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Natural products CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 3
- QFRYQWYZSQDFOS-UHFFFAOYSA-N verbascoside Natural products CC1OC(COC2C(O)C(COC3OC(C(O)C(O)C3O)C(=O)O)OC(Oc4cc(O)cc5OC(=CC(=O)c45)c6ccc(O)c(O)c6)C2O)C(O)C(O)C1O QFRYQWYZSQDFOS-UHFFFAOYSA-N 0.000 description 11
- KDSWDGKIENPKLB-QJDQKFITSA-N verbascoside Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](OC(=O)CCC=2C=C(O)C(O)=CC=2)[C@@H](CO)O[C@@H](OCCC=2C=C(O)C(O)=CC=2)[C@@H]1O KDSWDGKIENPKLB-QJDQKFITSA-N 0.000 description 10
- 229930182470 glycoside Natural products 0.000 description 5
- 150000002338 glycosides Chemical class 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 230000035784 germination Effects 0.000 description 3
- 238000003306 harvesting Methods 0.000 description 3
- 230000007226 seed germination Effects 0.000 description 3
- 235000009849 Cucumis sativus Nutrition 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 229930182486 flavonoid glycoside Natural products 0.000 description 2
- 150000007955 flavonoid glycosides Chemical class 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000293861 Scrophularia nodosa Species 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000005292 vacuum distillation Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Landscapes
- Saccharide Compounds (AREA)
Abstract
Invenţia se referă la agricultură, şi anume la un procedeu de tratare a seminţelor de castraveţi înainte de semănat.Procedeul, conform invenţiei, include înmuierea seminţelor de castraveţi timp de 24 ore într-o soluţie apoasă de 0,01% de dehidroconiferil-9-O-β-D-glucopiranozidă, obţinută din plante de Verbascum densiflorum Bertol. prin extragere cu soluţie hidroetanolică şi separare cromatografică.The invention relates to agriculture, namely a process for treating cucumber seeds before sowing. The process according to the invention includes soaking cucumber seeds for 24 hours in a 0.01% aqueous solution of dehydroconiferil-9-. O-β-D-glucopyranoside, obtained from plants of Verbascum densiflorum Bertol. by extraction with hydroethanol solution and chromatographic separation.
Description
Invenţia se referă la agricultură, şi anume la un procedeu de tratare a seminţelor de castraveţi înainte de semănat. The invention relates to agriculture, namely to a process for treating cucumber seeds before sowing.
Este cunoscut procedeul de tratare a seminţelor de castraveţi în soluţie apoasă de 0,001…0,01% de glicozide obţinute din planta Scrophularia nodosa L. (scrofulariozide) [1]. Dezavantajul acestui procedeu constă în faptul că tratarea seminţelor de castraveţi nu este suficient de efectivă. The process of treating cucumber seeds in an aqueous solution of 0.001…0.01% glycosides obtained from the plant Scrophularia nodosa L. (scrofulariosides) is known [1]. The disadvantage of this process is that the treatment of cucumber seeds is not sufficiently effective.
Problema pe care o rezolvă invenţia propusă constă în lărgirea sortimentului de substanţe biologic active cu efect major asupra activizării proceselor fiziologice, care sporesc semnificativ productivitatea şi calitatea recoltei de castraveţi. The problem solved by the proposed invention consists in expanding the range of biologically active substances with a major effect on the activation of physiological processes, which significantly increase the productivity and quality of the cucumber harvest.
Invenţia soluţionează problema prin aceea că se propune un procedeu de tratare a seminţelor de castraveţi înainte de semănat, care include înmuierea acestora timp de 24 ore într-o soluţie apoasă de 0,01% de dehidroconiferil-9-O-β-D-glucopiranozidă, obţinută din plante de Verbascum densiflorum Bertol. prin extragere cu soluţie hidroetanolică şi separare cromatografică. The invention solves the problem by proposing a process for treating cucumber seeds before sowing, which includes soaking them for 24 hours in an aqueous solution of 0.01% dehydroconiferyl-9-O-β-D-glucopyranoside, obtained from Verbascum densiflorum Bertol plants, by extraction with hydroethanolic solution and chromatographic separation.
Procedeul de obţinere a glicozidei flavonoide menţionate (în continuare - verbascozidă) constă în extragerea din plantele uscate cu etanol de 50% a complexului de glicozide, concentrarea extractului şi purificarea ulterioară a acestuia cu cloroform. Fracţiunea cu un conţinut predominant de glicozide cu valorile constantelor cromatografice Rf cuprinse între 0,83…0,75 a fost concentrată prin distilare în vid până la uscat şi separată prin cromatografiere pe silicagel şi Sephadex G-50. Identificarea verbascozidei cu Rf 0.78 se efectuează pe plăci de silufol în sistemul de solvenţi cloroform:metanol:apă (65:30:5) (Brevet MD 289, 2011). The process of obtaining the mentioned flavonoid glycoside (hereinafter - verbascoside) consists of extracting the glycoside complex from dried plants with 50% ethanol, concentrating the extract and its subsequent purification with chloroform. The fraction with a predominant content of glycosides with the values of the chromatographic constants Rf ranging from 0.83…0.75 was concentrated by vacuum distillation to dryness and separated by chromatography on silica gel and Sephadex G-50. The identification of verbascoside with Rf 0.78 is carried out on silufol plates in the solvent system chloroform:methanol:water (65:30:5) (Patent MD 289, 2011).
Rezultatul invenţiei constă în sporirea productivităţii castraveţilor, stimularea procesului de fructificare şi îmbunătăţirea calităţilor comerciale ale fructelor. The result of the invention consists in increasing the productivity of cucumbers, stimulating the fruiting process and improving the commercial qualities of the fruits.
Exemplu de realizare a invenţiei Example of embodiment of the invention
Cercetările s-au efectuat în condiţii de laborator şi în câmp deschis. În calitate de obiect de studiu au servit seminţele de castraveţi ale hibridului Ceceli cu termen mijlociu de coacere. The research was conducted in laboratory and open field conditions. The study object was cucumber seeds of the Ceceli hybrid with a medium ripening period.
În scopul determinării concentraţiei optime de verbascozidă, preventiv au fost efectuate testările biologice în condiţii de laborator. Seminţele de castraveţi au fost supuse înmuierii timp de 24 ore în soluţii apoase de verbascozidă în limitele concentraţiilor de 0,0001%...0,01%. In order to determine the optimal concentration of verbascoside, preventive biological tests were carried out under laboratory conditions. Cucumber seeds were soaked for 24 hours in aqueous solutions of verbascoside in concentrations ranging from 0.0001%...0.01%.
În baza rezultatelor obţinute la testare s-a stabilit că concentraţia optimă a soluţiei de verbascozidă pentru sporirea germinaţiei seminţelor de castraveţi este de 0,01% (tab. 1). Based on the results obtained during testing, it was established that the optimal concentration of verbascoside solution for increasing cucumber seed germination is 0.01% (Table 1).
Tabelul 1 Table 1
Influenţa glicozidelor asupra germinaţiei seminţelor de castraveţi The influence of glycosides on cucumber seed germination
Varianta Concentraţia, % Energia germinativă Germinaţia totală % % faţă de martor % % faţă de martor Martor 43,5 56,0 Scrofulariozide 0,0001 40,5 -6,9 51,8 -7,5 0,001 44,3 1,8 53,0 -5,4 0,005 48,8 12,2 61,0 8,9 0,01 44,3 1,8 53,3 -4,8 Verbascozidă 0,0001 43,5 0 62,5 11,6 0,001 37,0 -14,9 57,8 3,2 0,005 48,5 11,5 65,8 17,5 0,01 55,5 16,1 66,3 18,4Variant Concentration, % Germination energy Total germination % % compared to control % % compared to control Control 43.5 56.0 Scrofulariosides 0.0001 40.5 -6.9 51.8 -7.5 0.001 44.3 1.8 53.0 -5.4 0.005 48.8 12.2 61.0 8.9 0.01 44.3 1.8 53.3 -4.8 Verbascoside 0.0001 43.5 0 62.5 11.6 0.001 37.0 -14.9 57.8 3.2 0.005 48.5 11.5 65.8 17.5 0.01 55.5 16.1 66.3 18.4
În scopul determinării germinaţiei seminţelor de castraveţi în câmp deschis o parte din acestea au fost înmuiate în soluţie de verbascozidă cu concentraţia de 0,01% timp de 24 ore (invenţia propusă). Pentru comparaţie, cealaltă parte de seminţe a fost înmuiată în soluţie de 0,01% de scrofulariozidă timp de 24 ore (cea mai apropiată soluţie). În calitate de martor au servit seminţele înmuiate în apă. Seminţele din toate variantele, după înmuiere, au fost uscate şi ulterior semănate în câmp. Suprafaţa de evidenţă a experienţei a constituit 30 m2. Experienţa a fost efectuată în 4 repetiţii. Recoltarea castraveţilor s-a realizat multiplu － de 9 ori, pe tot parcursul perioadei de fructificare a culturii. In order to determine the germination of cucumber seeds in the open field, some of them were soaked in a solution of verbascoside with a concentration of 0.01% for 24 hours (the proposed invention). For comparison, the other part of the seeds was soaked in a solution of 0.01% scrofularioside for 24 hours (the closest solution). Seeds soaked in water served as a control. Seeds of all variants, after soaking, were dried and subsequently sown in the field. The experimental area was 30 m2. The experiment was carried out in 4 repetitions. Cucumber harvesting was carried out multiple times - 9 times, throughout the fruiting period of the crop.
De pe parcela, în care seminţele de castraveţi au fost tratate cu soluţie de verbascozidă de 0,01%, recolta totală de fructe a depăşit cu 35,3% varianta martor şi cu 43,8% varianta în care a fost utilizată soluţia de scrofulariozide (tab. 2). From the plot, where cucumber seeds were treated with 0.01% verbascoside solution, the total fruit harvest exceeded the control variant by 35.3% and the variant in which the scrofulariside solution was used by 43.8% (Table 2).
Tabelul 2 Table 2
Influenţa glicozidelor asupra procesului de fructificare şi productivităţii castraveţilor The influence of glycosides on the fruiting process and productivity of cucumbers
Varianta Numărul de fructe Productivitatea buc./m2 % faţă de martor t/ha % faţă de martor Martor 17 - 8,102 - Scrofulariozide 16 -5,9 7,520 -7,2 Verbascozidă 23 35,3 10,695 32,0Variant Number of fruits Productivity pcs./m2 % compared to control t/ha % compared to control Control 17 - 8.102 - Scrofulariosides 16 -5.9 7.520 -7.2 Verbascoside 23 35.3 10.695 32.0
Tratarea seminţelor de castraveţi înainte de semănat cu verbascozidă reduce semnificativ termenul de încolţire a seminţelor, ceea ce asigură, în condiţii de câmp, apariţia mai rapidă şi mai uniformă a plantelor şi ulterior sporirea productivităţii culturii de castraveţi. Treating cucumber seeds before sowing with verbascoside significantly reduces the seed germination period, which ensures, under field conditions, faster and more uniform emergence of plants and subsequently increases the productivity of the cucumber crop.
Planta Verbascum densiflorum Bertol. este frecvent răspândită în flora spontană a Republicii Moldova. Materia primă pentru obţinerea glicozidelor flavonoide, în special verbascozida, este accesibilă şi ieftină. The plant Verbascum densiflorum Bertol. is widely distributed in the spontaneous flora of the Republic of Moldova. The raw material for obtaining flavonoid glycosides, especially verbascoside, is accessible and cheap.
1. Vasilachi I., Gradinar D. Eficacitatea substanţelor biologic active în tehnologiile de cultivare a legumelor. Materialele conferinţei ştiinţifice internaţionale "Genetica, fiziologia şi ameliorarea plantelor", Ediţia V, Chişinău, 23...24 octombrie 2014, p. 467-472 1. Vasilachi I., Gradinar D. The effectiveness of biologically active substances in vegetable cultivation technologies. Materials of the international scientific conference "Genetics, physiology and plant breeding", V Edition, Chisinau, October 23...24, 2014, p. 467-472
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20140135A MD901Z (en) | 2014-10-31 | 2014-10-31 | Process for presowing treatment of cucumber seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20140135A MD901Z (en) | 2014-10-31 | 2014-10-31 | Process for presowing treatment of cucumber seeds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD901Y MD901Y (en) | 2015-05-31 |
| MD901Z true MD901Z (en) | 2015-12-31 |
Family
ID=53190462
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDS20140135A MD901Z (en) | 2014-10-31 | 2014-10-31 | Process for presowing treatment of cucumber seeds |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD901Z (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069078A (en) * | 2016-06-27 | 2016-11-09 | 安徽梅兰园林景观工程有限公司 | A kind of cucumber planting method |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD1042Z (en) * | 2015-11-25 | 2017-01-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for treating young pear trees |
| MD1111Z (en) * | 2016-08-01 | 2017-08-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for treating common wheat seeds with reduced viability |
| MD1213Z (en) * | 2017-06-24 | 2018-07-31 | Институт Микробиологии И Биотехнологии Академии Наук Молдовы | Process for growing cucumbers |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD754G2 (en) * | 1996-10-03 | 1998-04-30 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Process for cultivation of cucumbers |
| MD1032G2 (en) * | 1997-07-02 | 1999-06-30 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Chemical immunizator of cucumber plants |
| MD2688G2 (en) * | 2004-07-02 | 2005-10-31 | Центр Растительных Генетических Ресурсов Молдовы Академии Наук Республики Молдова | Process for treating cucumber seeds with reduced viability |
| MD289Y (en) * | 2010-07-27 | 2010-11-30 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Nutrient medium for germination of sweet pepper Capsicum annuum L. pollen |
| MD328Y (en) * | 2010-09-28 | 2011-02-28 | Inst De Genetica Si Fiziol A Plantelor Al Academiei De Stiinte A Moldovei | Process for presowing treatment of sweet pepper seeds |
| MD862Y (en) * | 2014-08-15 | 2015-01-31 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | Process for growing tobacco plants |
-
2014
- 2014-10-31 MD MDS20140135A patent/MD901Z/en not_active IP Right Cessation
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD754G2 (en) * | 1996-10-03 | 1998-04-30 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Process for cultivation of cucumbers |
| MD1032G2 (en) * | 1997-07-02 | 1999-06-30 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Chemical immunizator of cucumber plants |
| MD2688G2 (en) * | 2004-07-02 | 2005-10-31 | Центр Растительных Генетических Ресурсов Молдовы Академии Наук Республики Молдова | Process for treating cucumber seeds with reduced viability |
| MD289Y (en) * | 2010-07-27 | 2010-11-30 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Nutrient medium for germination of sweet pepper Capsicum annuum L. pollen |
| MD328Y (en) * | 2010-09-28 | 2011-02-28 | Inst De Genetica Si Fiziol A Plantelor Al Academiei De Stiinte A Moldovei | Process for presowing treatment of sweet pepper seeds |
| MD862Y (en) * | 2014-08-15 | 2015-01-31 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | Process for growing tobacco plants |
Non-Patent Citations (1)
| Title |
|---|
| Vasilachi I., Gradinar D. Eficacitatea substanţelor biologic active în tehnologiile de cultivare a legumelor. Materialele conferinţei ştiinţifice internaţionale "Genetica, fiziologia şi ameliorarea plantelor", Ediţia V, Chişinău, 23...24 octombrie 2014, p. 467-472 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069078A (en) * | 2016-06-27 | 2016-11-09 | 安徽梅兰园林景观工程有限公司 | A kind of cucumber planting method |
Also Published As
| Publication number | Publication date |
|---|---|
| MD901Y (en) | 2015-05-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Caruso et al. | Quality performances of sweet pepper under farming management | |
| US10674730B2 (en) | Method for stimulation of seeds | |
| MD901Z (en) | Process for presowing treatment of cucumber seeds | |
| Rashad et al. | Antifungal potential and defense gene induction in maize against Rhizoctonia root rot by seed extract of Ammi visnaga (L.) Lam. | |
| Nimmi et al. | Effect of pre-sowing treatment with permanent magnetic field on germination and growth of chilli [Capsicum annum L.] | |
| Sharma et al. | Effect of growth regulator sprays on growth, yield and quality of guava under malwa plateau conditions | |
| Ramos-Solano et al. | Bacterial bioeffectors delay postharvest fungal growth and modify total phenolics, flavonoids and anthocyanins in blackberries | |
| MD636Z (en) | Process for producing flavonoid glycosides from Linaria vulgaris Mill. | |
| Viškelis et al. | Triterpenic acid content in the fruit peel of Malus× domestica Borkh. depends on the growing technology | |
| MD893Z (en) | Process for presowing treatment of onion seeds | |
| Hirve et al. | Effect of Cadmium exposures on growth and biochemical parameters of Vigna radiata seedlings | |
| MD862Z (en) | Process for growing tobacco plants | |
| Reddy et al. | Management of fruit rot of Chilli caused by Colletotrichum capsici | |
| MD922Z (en) | Process for pre-sowing treatment of carrot seeds | |
| MD153Y (en) | Process for treating chick pea seeds with reduced viability | |
| RU2480977C2 (en) | Method for stimulation of vegetable crops growth and development | |
| Abhijith et al. | Effect of micronutrients on growth and yield of aonla (Emblicaofficinalis gaertn.) CV. Na-7 | |
| Fohs et al. | The effect of cultivation ways on selected morphological characters of bitter gourd (Momordica charantia L.) transplants and plants, fruit yield and chemical content. | |
| Tanase et al. | Wastes from forestry and energy industries as potential bioregulators in soybean (Glycine max L.) plants | |
| RU2626174C1 (en) | Agent for pre-sowing treatment of vegetable crop seeds in protected soil conditions | |
| MD1188Y (en) | Process for presowing treatment of common onion seeds | |
| RU2214710C2 (en) | Preparation of growth-stimulating action for protecting plants against diseases | |
| MD767Z (en) | Process for treating vines | |
| Kaya et al. | Extraction of Plant Growth Regulators and Their Impact on the Growth Processes of Cereal Crops | |
| Mensah et al. | Mutagenic effects of hydroxylamine and hydroquinone on some agronomic and yield characters of soyabean (Glycine max (L.) Merr.) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG9Y | Short term patent issued | ||
| KA4Y | Short-term patent lapsed due to non-payment of fees (with right of restoration) |