MD922Z - Process for pre-sowing treatment of carrot seeds - Google Patents
Process for pre-sowing treatment of carrot seeds Download PDFInfo
- Publication number
- MD922Z MD922Z MDS20150007A MDS20150007A MD922Z MD 922 Z MD922 Z MD 922Z MD S20150007 A MDS20150007 A MD S20150007A MD S20150007 A MDS20150007 A MD S20150007A MD 922 Z MD922 Z MD 922Z
- Authority
- MD
- Moldova
- Prior art keywords
- seeds
- carrot
- carrot seeds
- glycosides
- iridoid glycosides
- Prior art date
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- 244000000626 Daucus carota Species 0.000 title claims abstract description 16
- 235000002767 Daucus carota Nutrition 0.000 title claims abstract description 16
- 238000000034 method Methods 0.000 title claims abstract description 11
- 238000009331 sowing Methods 0.000 title claims abstract description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000003480 eluent Substances 0.000 claims abstract description 7
- 239000007864 aqueous solution Substances 0.000 claims abstract description 6
- 238000000605 extraction Methods 0.000 claims abstract description 6
- 229930182489 iridoid glycoside Natural products 0.000 claims abstract description 6
- 150000008145 iridoid glycosides Chemical class 0.000 claims abstract description 6
- 241000155655 Melampyrum nemorosum Species 0.000 claims abstract description 5
- 238000002791 soaking Methods 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 229920005654 Sephadex Polymers 0.000 claims abstract description 4
- 239000012507 Sephadex™ Substances 0.000 claims abstract description 4
- 238000001704 evaporation Methods 0.000 claims abstract description 4
- 230000008020 evaporation Effects 0.000 claims abstract description 4
- 238000002523 gelfiltration Methods 0.000 claims abstract description 4
- 238000010438 heat treatment Methods 0.000 claims abstract description 4
- 238000000926 separation method Methods 0.000 claims abstract description 3
- 239000000243 solution Substances 0.000 description 7
- 230000035784 germination Effects 0.000 description 6
- 229930182470 glycoside Natural products 0.000 description 6
- 150000002338 glycosides Chemical class 0.000 description 5
- JNNRILAYMZYEQB-UHFFFAOYSA-N 6,4'-dimethoxy-5-hydroxyflavone 7-glucoside Natural products C1=CC(OC)=CC=C1C(OC1=C2)=CC(=O)C1=C(O)C(OC)=C2OC1C(O)C(O)C(O)C(CO)O1 JNNRILAYMZYEQB-UHFFFAOYSA-N 0.000 description 3
- 235000019441 ethanol Nutrition 0.000 description 3
- 238000003306 harvesting Methods 0.000 description 3
- JNNRILAYMZYEQB-FZFRBNDOSA-N 5-hydroxy-6-methoxy-2-(4-methoxyphenyl)-7-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-4-one Chemical compound C1=CC(OC)=CC=C1C(OC1=C2)=CC(=O)C1=C(O)C(OC)=C2O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JNNRILAYMZYEQB-FZFRBNDOSA-N 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000007226 seed germination Effects 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241000167860 Linaria vulgaris Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- -1 linaroside glycosides Chemical class 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
Description
Invenţia se referă la agricultură, şi anume la un procedeu de tratare a seminţelor de morcov înainte de semănat. The invention relates to agriculture, namely to a process for treating carrot seeds before sowing.
Este cunoscut procedeul de sporire a germinaţiei seminţelor de morcov în câmp deschis, care constă în înmuierea acestora în soluţia apoasă a glicozidelor linarozide, extrase din planta florei spontane Linaria vulgaris L., de 0,0001% timp de 24 ore. The process of increasing the germination of carrot seeds in the open field is known, which consists of soaking them in an aqueous solution of linaroside glycosides, extracted from the spontaneous flora plant Linaria vulgaris L., of 0.0001% for 24 hours.
Dezavantajul acestui procedeu constă în faptul că eficacitatea metodei de extragere a glicozidei nu a fost suficient verificată [1]. The disadvantage of this process is that the effectiveness of the glycoside extraction method has not been sufficiently verified [1].
Problema pe care o rezolvă invenţia constă în lărgirea sortimentului de substanţe biologic active cu efect major asupra activizării proceselor fiziologice, care, respectiv, majorează semnificativ productivitatea şi calitatea recoltei de morcov. The problem solved by the invention consists in expanding the assortment of biologically active substances with a major effect on the activation of physiological processes, which, respectively, significantly increases the productivity and quality of the carrot harvest.
Procedeul de tratare a seminţelor de morcov înainte de semănat include înmuierea seminţelor înainte de semănat timp de 15 min într-o soluţie apoasă de 0,01% de glicozide iridoidice, obţinute din plante de Melampyrum nemorosum L. prin extragere la încălzire cu etanol de 60%, separare prin gel-filtrare pe sephadex G-25 şi G-50, utilizând apa în calitate de eluent, unirea eluenţilor, conţinând glicozide iridoidice, şi evaporarea în vid. The process of treating carrot seeds before sowing includes soaking the seeds before sowing for 15 min in an aqueous solution of 0.01% iridoid glycosides, obtained from Melampyrum nemorosum L. plants by extraction with heating with 60% ethanol, separation by gel filtration on sephadex G-25 and G-50, using water as eluent, combining the eluents, containing iridoid glycosides, and evaporation in vacuo.
Rezultatul constă în sporirea germinaţiei seminţelor de morcov în câmp deschis, care asigură răsărirea şi creşterea uniformă a plantulelor, precum şi sporirea recoltei. The result is increased germination of carrot seeds in the open field, which ensures uniform emergence and growth of seedlings, as well as increased harvest.
Metoda de obţinere a sumei de melampirozide iridoidice constă în următoarele operaţiuni: plantele de Melampyrum nemorosum L., uscate în aer liber şi fărâmiţate, au fost supuse extracţiei prin încălzire (t=80°C) cu alcool etilic de 60% timp de 6 ore. Extracţia a fost repetată de 3 ori. Etanolul a fost distilat din extract, iar reziduul apos obţinut a fost supus gel-filtrării prin sephadex de tip G-25 şi G-50, utilizând apa distilată în calitate de eluent. Procesul eluării se controlează permanent prin cromatografierea în strat subţire de silicagel. Eluenţii, care conţin glicozide din seria iridoică, au fost uniţi şi concentraţi prin evaporare în vid, după care au fost uscaţi. Ca rezultat s-a obţinut suma a patru glicozide numite melampirozide cu Rf = 1,8; 0,28; 0,39; 0,6 în sistemul de solvenţi sub formă de pulbere de culoare brună deschisă. The method for obtaining the sum of iridoid melampyrosides consists of the following operations: Melampyrum nemorosum L. plants, dried in the open air and crushed, were subjected to extraction by heating (t=80°C) with 60% ethyl alcohol for 6 hours. The extraction was repeated 3 times. The ethanol was distilled from the extract, and the aqueous residue obtained was subjected to gel filtration through sephadex type G-25 and G-50, using distilled water as eluent. The elution process is permanently controlled by silica gel thin layer chromatography. The eluents, which contain glycosides from the iridoic series, were combined and concentrated by evaporation in vacuum, after which they were dried. As a result, the sum of four glycosides called melampyrosides with Rf = 1.8; 0.28; 0.39; 0.6 in the solvent system as a light brown powder.
Exemplu de realizare a invenţiei Example of embodiment of the invention
Cercetările s-au desfăşurat în condiţii de laborator şi în câmp deschis. În calitate de obiect de studiu a servit soiul de morcov Krasavka. În scopul determinării concentraţiei optimale de melampirozide, preventiv a fost efectuată experienţa în laborator. Seminţele de morcov au fost supuse înmuierii timp de 24 ore în soluţii apoase de melampirozide în limitele concentraţiilor de 0,0001...0,01%. The research was conducted in laboratory conditions and in the open field. The object of study was the carrot variety Krasavka. In order to determine the optimal concentration of melampyrozide, a laboratory experiment was conducted as a preventive measure. Carrot seeds were soaked for 24 hours in aqueous solutions of melampyrozide in the concentration range of 0.0001...0.01%.
Rezultatele obţinute ne permit să concluzionăm că concentraţia optimă a soluţiei de melampirozide pentru sporirea germinaţiei seminţelor şi lungimii plantulelor de morcov este de 0,01% (tab. 1). The results obtained allow us to conclude that the optimal concentration of melampyrozide solution for increasing seed germination and carrot seedling length is 0.01% (Table 1).
În scopul determinării germinaţiei seminţelor de morcov în câmp deschis o parte din acestea au fost înmuiate în soluţie de melampirozide în concentraţie de 0,01% timp de 15 min (invenţia propusă). Pentru comparaţie, cealaltă parte din seminţe a fost înmuiată în soluţie de 0,01% de linarozide timp de 15 min (cea mai apropiată soluţie). În calitate de martor au servit seminţele înmuiate în apă. Seminţele din toate variantele după înmuiere au fost uscate şi ulterior semănate în câmp cu ajutorul semănătoarei pentru legume. Experienţa a fost efectuată în 4 repetări. In order to determine the germination of carrot seeds in the open field, some of them were soaked in a solution of melampyrozide at a concentration of 0.01% for 15 min (the proposed invention). For comparison, the other part of the seeds was soaked in a solution of 0.01% linaroside for 15 min (the closest solution). Seeds soaked in water served as a control. Seeds of all variants after soaking were dried and subsequently sown in the field using a vegetable seeder. The experiment was carried out in 4 repetitions.
Tabelul 1 Table 1
Influenţa glicozidelor asupra germinaţiei seminţelor de morcov The influence of glycosides on carrot seed germination
Varianta Concen-traţia, % Energia germinativă Germinaţia totală Lungimea plantulelor % % faţă de martor % % faţă de martor % % faţă de martor Martor 62,0 69,3 4,4 Linarozide 0,0001 66,3 6,9 74,3 7,2 4,9 11,4 0,001 69,3 11,8 76,5 10,4 4,7 6,8 0,005 63,8 2,9 71,8 3,6 4,5 2,3 0,01 58,0 -6,5 66,8 -3,7 4,8 9,1 Melampirozide 0,0001 68,0 9,8 74,3 7,2 4,1 -6,2 0,001 72,8 17,4 79,5 14,7 5,0 13,6 0,005 75,8 22,3 81,8 18,0 5,2 18,2 0,01 72,3 16,6 78,5 13,3 6,1 38,6Variant Concentration, % Germination energy Total germination Seedling length % % compared to control % % compared to control % % compared to control Control 62.0 69.3 4.4 Linarosides 0.0001 66.3 6.9 74.3 7.2 4.9 11.4 0.001 69.3 11.8 76.5 10.4 4.7 6.8 0.005 63.8 2.9 71.8 3.6 4.5 2.3 0.01 58.0 -6.5 66.8 -3.7 4.8 9.1 Melampyrosides 0.0001 68.0 9.8 74.3 7.2 4.1 -6.2 0.001 72.8 17.4 79.5 14.7 5.0 13.6 0.005 75.8 22.3 81.8 18.0 5.2 18.2 0.01 72.3 16.6 78.5 13.3 6.1 38.6
Trebuie menţionat faptul că tratarea seminţelor de morcov cu soluţie apoasă de melampirozide a favorizat germinaţia asigurând astfel apariţia şi dezvoltarea uniformă a plantulelor. Efectul în cauză a influenţat pozitiv asupra productivităţii. It should be noted that treating carrot seeds with an aqueous solution of melampyrozide favored germination, thus ensuring the emergence and uniform development of seedlings. This effect had a positive influence on productivity.
De pe parcela, în care seminţele de morcov au fost tratate cu 0,01% soluţie de melampirozide, recolta totală de fructe a depăşit cu 41,4% (6,7 t/ha) varianta martor şi cu 13,9% (2,8 t/ha) varianta în care a fost utilizată soluţia de linarozide (tab. 2). From the plot, where carrot seeds were treated with 0.01% melampyrozide solution, the total fruit harvest exceeded by 41.4% (6.7 t/ha) the control variant and by 13.9% (2.8 t/ha) the variant in which the linaroside solution was used (tab. 2).
Tabelul 2 Table 2
Influenţa glicozidelor asupra productivităţii culturii de morcov The influence of glycosides on carrot crop productivity
Varianta Productivitatea t/ha % faţă de martor Martor 16,2 Linarozide 20,1 24,1 Melampirozide 22,9 41,4Variant Productivity t/ha % compared to control Control 16.2 Linarosides 20.1 24.1 Melampyrosides 22.9 41.4
Aşadar, procedeul propus, în comparaţie cu cea mai apropiată soluţie, este mult mai efectiv, substanţa este obţinută din planta Melampyrum nemorosum L., care se găseşte în cantităţi suficiente în flora spontană. Therefore, the proposed process, compared to the closest solution, is much more effective, the substance is obtained from the Melampyrum nemorosum L. plant, which is found in sufficient quantities in the spontaneous flora.
1. Мащенко Н. Е., Боровская А. Д. Потенциальные возможности применения регуляторов роста растительного происхождения в овощеводстве. Материалы XI международной научно-методической конференции Интродукция, сохранение и использование биологического разнообразия культурных растений, 9-19 июня 2014, Махачкала, V. I, р. 30-34 1. Mashchenko N. E., Borovskaya A. D. Potential opportunities of application of plant growth regulators in fruit growing. Materials XI international scientific-methodical conference Introduction, preservation and use of biological diversity of cultural plants, 9-19 June 2014, Makhachkala, V. I, р. 30-34
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20150007A MD922Z (en) | 2015-01-28 | 2015-01-28 | Process for pre-sowing treatment of carrot seeds |
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| MDS20150007A MD922Z (en) | 2015-01-28 | 2015-01-28 | Process for pre-sowing treatment of carrot seeds |
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| MD922Y MD922Y (en) | 2015-07-31 |
| MD922Z true MD922Z (en) | 2016-02-29 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| MD1188Z (en) * | 2017-03-15 | 2018-04-30 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for presowing treatment of common onion seeds |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD830F1 (en) * | 1997-02-14 | 1997-09-30 | Holdingul De Stat Avicola | Hen subrace "Gat golas moldovenesc heavy type" |
| MD1163G2 (en) * | 1997-07-02 | 1999-10-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Process for presowing carrots seeds treetment |
| RU2001128040A (en) * | 1999-03-13 | 2003-08-20 | Авентис Кропсайенс Гмбх (De) | SEED TREATMENT COMPOSITION |
| EA013410B1 (en) * | 2005-06-16 | 2010-04-30 | Басф Акциенгезельшафт | Use of phenylsemicarbazones for seed treatment |
| MD365Y (en) * | 2011-01-10 | 2011-05-31 | Inst De Genetica Si Fiziol A Plantelor Al Academiei De Stiinte A Moldovei | Process for presowing treatment of carrot seeds |
| EA020590B1 (en) * | 2006-10-16 | 2014-12-30 | Монсанто Текнолоджи Ллс | Method for improving plant health |
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2015
- 2015-01-28 MD MDS20150007A patent/MD922Z/en not_active IP Right Cessation
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD830F1 (en) * | 1997-02-14 | 1997-09-30 | Holdingul De Stat Avicola | Hen subrace "Gat golas moldovenesc heavy type" |
| MD1163G2 (en) * | 1997-07-02 | 1999-10-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Process for presowing carrots seeds treetment |
| RU2001128040A (en) * | 1999-03-13 | 2003-08-20 | Авентис Кропсайенс Гмбх (De) | SEED TREATMENT COMPOSITION |
| EA013410B1 (en) * | 2005-06-16 | 2010-04-30 | Басф Акциенгезельшафт | Use of phenylsemicarbazones for seed treatment |
| EA020590B1 (en) * | 2006-10-16 | 2014-12-30 | Монсанто Текнолоджи Ллс | Method for improving plant health |
| MD365Y (en) * | 2011-01-10 | 2011-05-31 | Inst De Genetica Si Fiziol A Plantelor Al Academiei De Stiinte A Moldovei | Process for presowing treatment of carrot seeds |
Non-Patent Citations (1)
| Title |
|---|
| Мащенко Н. Е., Боровская А. Д. Потенциальные возможности применения регуляторов роста растительного происхождения в овощеводстве. Материалы XI международной научно-методической конференции Интродукция, сохранение и использование биологического разнообразия культурных растений, 9-19 июня 2014, Махачкала, V. I, р. 30-34 * |
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| Publication number | Publication date |
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| MD922Y (en) | 2015-07-31 |
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