MD1042Z - Process for treating young pear trees - Google Patents
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- MD1042Z MD1042Z MDS20150155A MDS20150155A MD1042Z MD 1042 Z MD1042 Z MD 1042Z MD S20150155 A MDS20150155 A MD S20150155A MD S20150155 A MDS20150155 A MD S20150155A MD 1042 Z MD1042 Z MD 1042Z
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- 238000000034 method Methods 0.000 title claims abstract description 20
- 240000001987 Pyrus communis Species 0.000 title abstract 2
- 235000014443 Pyrus communis Nutrition 0.000 title abstract 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims abstract description 12
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000000243 solution Substances 0.000 claims abstract description 9
- 239000007864 aqueous solution Substances 0.000 claims abstract description 7
- 241000293859 Verbascum phlomoides Species 0.000 claims abstract description 6
- 238000000605 extraction Methods 0.000 claims abstract description 6
- 229930182470 glycoside Natural products 0.000 claims abstract description 6
- 150000002338 glycosides Chemical class 0.000 claims abstract description 6
- 238000009835 boiling Methods 0.000 claims abstract description 4
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims abstract description 4
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- 235000010599 Verbascum thapsus Nutrition 0.000 claims description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 abstract description 6
- QFRYQWYZSQDFOS-UHFFFAOYSA-N verbascoside Natural products CC1OC(COC2C(O)C(COC3OC(C(O)C(O)C3O)C(=O)O)OC(Oc4cc(O)cc5OC(=CC(=O)c45)c6ccc(O)c(O)c6)C2O)C(O)C(O)C1O QFRYQWYZSQDFOS-UHFFFAOYSA-N 0.000 description 11
- 239000000049 pigment Substances 0.000 description 9
- JNNRILAYMZYEQB-FZFRBNDOSA-N 5-hydroxy-6-methoxy-2-(4-methoxyphenyl)-7-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-4-one Chemical compound C1=CC(OC)=CC=C1C(OC1=C2)=CC(=O)C1=C(O)C(OC)=C2O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JNNRILAYMZYEQB-FZFRBNDOSA-N 0.000 description 8
- JNNRILAYMZYEQB-UHFFFAOYSA-N 6,4'-dimethoxy-5-hydroxyflavone 7-glucoside Natural products C1=CC(OC)=CC=C1C(OC1=C2)=CC(=O)C1=C(O)C(OC)=C2OC1C(O)C(O)C(O)C(CO)O1 JNNRILAYMZYEQB-UHFFFAOYSA-N 0.000 description 8
- 230000029553 photosynthesis Effects 0.000 description 8
- 238000010672 photosynthesis Methods 0.000 description 8
- 235000013399 edible fruits Nutrition 0.000 description 7
- KDSWDGKIENPKLB-QJDQKFITSA-N verbascoside Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](OC(=O)CCC=2C=C(O)C(O)=CC=2)[C@@H](CO)O[C@@H](OCCC=2C=C(O)C(O)=CC=2)[C@@H]1O KDSWDGKIENPKLB-QJDQKFITSA-N 0.000 description 7
- 235000021466 carotenoid Nutrition 0.000 description 5
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- 229930002875 chlorophyll Natural products 0.000 description 5
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- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 5
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- 229930182486 flavonoid glycoside Natural products 0.000 description 4
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Изобретение относится к сельскому хозяйству, а именно к способу обработки молодых деревьев груши.Способ, согласно изобретению, включает внекорневую обработку деревьев 0,01% водным раствором гликозидов, полученных из Verbascum phlomoides L. путем экстрагирования при кипячении 80%-ным воднометанольным раствором, экстрагирования этилацетатом, осаждения хлороформом и хроматографического сепарирования фракции с Rf 0,64…0,5, при этом обработка осуществляется через 2…3 недели после цветения с общим расходом 800…1000 л/га.The invention relates to agriculture, and in particular to a method for processing young pear trees. The method according to the invention includes foliar treatment of trees with a 0.01% aqueous solution of glycosides obtained from Verbascum phlomoides L. by boiling extraction with an 80% aqueous methanol solution, extraction ethyl acetate, precipitation with chloroform and chromatographic separation of the fraction with Rf of 0.64 ... 0.5, while processing is carried out 2 ... 3 weeks after flowering with a total flow of 800 ... 1000 l / ha.
Description
Invenţia se referă la agricultură, în special la un procedeu de tratare a pomilor tineri de păr. The invention relates to agriculture, in particular to a process for treating young hair trees.
Este cunoscut procedeul de tratare a pomilor fructiferi în perioada creşterii vegetative intensive cu soluţie apoasă a glicozidei flavonoidice steroidice 5,4-dimetilchempferol 3-O-β-D (6′′-α-L-ramnopiranosil)-glucopiranozidă (linarozidă) în concentraţie de 0,01%, cu un consum de 0,20…0,25 L/pom [1]. Însă acest procedeu se caracterizează printr-o eficacitate scăzută. The process of treating fruit trees during the period of intensive vegetative growth with an aqueous solution of the steroidal flavonoid glycoside 5,4-dimethylchempferol 3-O-β-D (6′′-α-L-rhamnopyranosyl)-glucopyranoside (linaroside) in a concentration of 0.01%, with a consumption of 0.20…0.25 L/tree is known [1]. However, this process is characterized by low efficacy.
Problema pe care o rezolvă invenţia propusă constă în intensificarea proceselor fiziologice, în particular a proceselor de fotosinteză şi acumulare a pigmenţilor, precum şi a creşterii şi dezvoltării pomilor, fapt ce poate contribui în mare măsură la sporirea productivităţii pomilor de păr şi a calităţii recoltei de fructe. The problem solved by the proposed invention consists in intensifying physiological processes, in particular the processes of photosynthesis and pigment accumulation, as well as the growth and development of trees, which can greatly contribute to increasing the productivity of hair trees and the quality of the fruit harvest.
Procedeul de tratare a pomilor tineri de păr, conform invenţiei propuse, include tratarea extraradiculară a pomilor cu o soluţie apoasă de 0,01% de glicozide obţinute din Verbascum phlomoides L. prin extragere la fierbere cu soluţie hidrometanolică de 80%, extragere cu acetat de etil, precipitare cu cloroform şi separare cromatografică a fracţiunii cu Rf 0,64…0,5, totodată tratarea se efectuează peste 2…3 săptămâni după înflorire cu un consum total de 800…1000 L/ha. The process for treating young mullein trees, according to the proposed invention, includes extra-radicular treatment of the trees with a 0.01% aqueous solution of glycosides obtained from Verbascum phlomoides L. by boiling extraction with 80% hydromethanolic solution, extraction with ethyl acetate, precipitation with chloroform and chromatographic separation of the fraction with Rf 0.64…0.5, at the same time the treatment is carried out 2…3 weeks after flowering with a total consumption of 800…1000 L/ha.
Rezultatul tehnic constă în intensificarea proceselor de fotosinteză şi acumulare a pigmenţilor, sporirea creşterii vegetative a lăstarilor, care va contribui la iniţierea mugurilor de rod la pomii soiurilor tardive de păr pentru roada anului viitor. The technical result consists in intensifying the processes of photosynthesis and pigment accumulation, increasing the vegetative growth of shoots, which will contribute to the initiation of fruit buds on trees of late hair varieties for next year's harvest.
Glicozidele obţinute din Verbascum phlomoides L. (numite verbascozide) au fost obţinute în felul următor: partea aeriană a plantei Verbascum phlomoides a fost mărunţită şi extrasă cu soluţie hidrometanolică de 80% de 3 ori prin fierbere. Extractele au fost unite şi concentrate prin distilare în vid. Reziduul apos a fost extras cu acetat de etil, iar extractele obţinute au fost concentrate până la rest apos prin distilare în vid. Din restul apos au fost precipitate cu cloroform glicozidele flavanoidice sumare, numite verbascozide, care au fost cromatografiate multiplu pe coloane cu poliamidă. Coloanele au fost eluate cu cloroform, apoi cu amestec de cloroform: metanol în raport volumetric de 13:7, eluatele au fost analizate prin cromatografiere în strat subţire, cele cu conţinut identic au fost unite şi concentrate prin evaporare până la uscat. Ca rezultat s-au obţinut glicozide flavonoidice - verbascozidele. Fracţiunea cu un conţinut predominant de glicozide cu valorile constantelor cromatografice Rf cuprinse între 0,64…0,50 a fost concentrată prin distilare în vid până la uscat. În rezultatul uscat au fost detectate cu ajutorul cromatografiei în strat subţire de silicagel două glicozide flavonoidice majore, structura cărora a fost stabilită după separare prin cromatografiere pe coloane cu poliamidă, cu ajutorul procedeelor fizico-chimice, spectroscopiei H şi BC RMN. Astfel, în extractul sumar au fost identificate prin comparare cu datele bibliografice următoarele substanţe: 3-chempferolul (Gang Chen, Huiri Lin, Xuefend Li et al. Химия природных соединений, p. 607, 2009) şi apigenin-7-O-glucopiranozida (Lhu X.F., Zhang H.X. Химия природных соединений, p. 801, 1972). The glycosides obtained from Verbascum phlomoides L. (called verbascosides) were obtained in the following way: the aerial part of the Verbascum phlomoides plant was crushed and extracted with 80% hydromethanolic solution 3 times by boiling. The extracts were combined and concentrated by vacuum distillation. The aqueous residue was extracted with ethyl acetate, and the extracts obtained were concentrated to an aqueous residue by vacuum distillation. From the aqueous residue, the summary flavonoid glycosides, called verbascosides, were precipitated with chloroform, which were chromatographed multiple times on polyamide columns. The columns were eluted with chloroform, then with a mixture of chloroform: methanol in a volumetric ratio of 13:7, the eluates were analyzed by thin layer chromatography, those with identical content were combined and concentrated by evaporation to dryness. As a result, flavonoid glycosides - verbascosides were obtained. The fraction with a predominant content of glycosides with the values of the chromatographic constants Rf ranging from 0.64…0.50 was concentrated by vacuum distillation to dryness. In the dry result, two major flavonoid glycosides were detected using silica gel thin layer chromatography, the structure of which was established after separation by chromatography on polyamide columns, using physicochemical procedures, H and BC NMR spectroscopy. Thus, the following substances were identified in the summary extract by comparison with bibliographic data: 3-chempferol (Gang Chen, Huiri Lin, Xuefend Li et al. Химия природных соединения, p. 607, 2009) and apigenin-7-O-glucopyranoside (Lhu X.F., Zhang H.X. Химия природных соединения, p. 801, 1972).
Avantajele procedeului propus faţă de procedeul cunoscut sunt: intensificarea proceselor de fotosinteză a aparatului foliar cu majorarea conţinutului de pigmenţi clorofilieni, sporirea creşterii vegetative a lăstarilor şi, îndeosebi, a proceselor de depunere a mugurilor floriferi pentru roada anului viitor. The advantages of the proposed process over the known process are: intensification of photosynthesis processes of the leaf apparatus with an increase in the content of chlorophyll pigments, enhancement of vegetative growth of shoots and, especially, of the processes of laying flower buds for next year's fruit.
Exemple de realizare a invenţiei Examples of embodiments of the invention
Experienţele au fost montate într-o livadă de păr la pomi tineri cu vârsta de 3 ani de soiul Noiabriskaia, până la intrarea lor pe rod. O parte din pomii de păr după înflorire au fost trataţi extraradicular cu 0,01% soluţie apoasă de verbascozide. Pentru comparaţie o altă parte de pomi de acelaşi soi au fost trataţi cu 0,01% soluţie apoasă de linarozidă. În calitate de martor au servit pomii trataţi cu apă distilată. Consumul de soluţie a constituit 0,20…0,25 L/pom. The experiments were set up in a 3-year-old apple orchard with young trees of the Noiabriskaia variety, until they began to bear fruit. Some of the apple trees after flowering were treated extra-radicularly with 0.01% aqueous solution of verbascoside. For comparison, another part of the trees of the same variety were treated with 0.01% aqueous solution of linaroside. Trees treated with distilled water served as controls. The solution consumption was 0.20…0.25 L/tree.
În dinamica perioadei de vegetaţie s-a determinat acţiunea regulatorului de creştere verbascozide ( procedeul propus) şi linarozidă (cea mai apropiată soluţii) asupra intensităţii proceselor de fotosinteză, de acumulare a pigmenţilor clorofilieni şi a carotenoizilor, precum şi asupra creşterii lăstarilor anuali pe durata perioadei de estimare. Rezultatele obţinute sunt prezentate în tabel. In the dynamics of the vegetation period, the action of the growth regulator verbascoside (the proposed procedure) and linaroside (the closest solution) on the intensity of photosynthesis processes, accumulation of chlorophyll pigments and carotenoids, as well as on the growth of annual shoots during the estimation period was determined. The results obtained are presented in the table.
Tabel Table
Data estimării, variantă Unii indici fiziologici evaluaţi pe parcursul vegetaţiei Fotosinteza, µmol/dm-2/sec-1 Pigmenţii clorofilieni, mg/ dm-2 Conţinutul pigmen- ţilor carotenoizi, mg/ dm-2 Creşterea lăstarilor apicali, cm Creşterea lăstarilor anuali în raport cu dimensiunile iniţiale, % a b suma 14.05.2015 1. Martor 2. Linarozidă 0,01% 3.Verbascozide 0,01% - - - - - 8,0±1,7 14,0±4,3 7,0±2,7 0,00 0,00 0,00 04.06.2015 1. Martor 2. Linarozidă 0,01% 3.Verbascozide 0,01% 3,08±0,45 2,49± 0,45 2,32± 014 1,65±0,08 1,82±0,04 1,84±0,03 0,35±0,05 0,46 ±0,02 0,45 ±0,02 2,00±0,02 2,28±0,01 2,29±0,05 0,69±0,05 0,71±0,06 0,75±0,02 14.2±5,5 24.0±4,3 21.0±1,5 77,5±43,6 71,4±49,7 200,0±78,8 18.06.2015 1. Martor 2. Linarozidă 0,01% 3.Verbascozide 0,01% 1,85± 0,06 2,33 ±0,04 3,38± 0,35 2,21±0,05 2,47±0,06 2,61±0,09 0,41±0,02 0,57±0,03 0,60±0,03 2,64±0,07 3,04±0,04 3,21±0,11 0,92±0,03 1,00±0,04 1,03±0,04 14,5±6,7 26,0±4,8 23,1±1,9 81,3±51,0 85,7±46,6 230,0±97,7 02.07.2015 1. Martor 2. Linarozidă 0,01% 3.Verbascozide 0,01% 1,83± 0,60 1,10± 0,08 3,14± 0,08 2,25±0,01 2,40±0,08 2,72±0,05 0,54±0,06 0,55±0,01 0,75±0,03 2,79±0,01 2,95±0,03 3,47±0,00 0,88±0,07 0,80±0,03 1,09±0,04 14,5±9,4 33,8±9,9 23,3±0,6 81,3±70,6 141,4±10,5 233,0±81,1 17.07.2015 1. Martor 2. Linarozidă 0,01% 3.Verbascozide 0,01% 1,55 ±0,19 2,57±0,16 2,94± 0,15 2,37±0,02 2,32±0,04 2,85±0,08 0,64±0,08 0,55±0,01 0,75±0,04 3,01±0,07 2,87±0,06 3,06±0,14 1,18±0,03 1,01±0,02 1,17±0,04 14,5 ±9,4 33,8±9,9 23,3 ±0,6 81,3±70,6 141,4±10,5 233,0±81,1Estimation date, variant Some physiological indices evaluated during vegetation Photosynthesis, µmol/dm-2/sec-1 Chlorophyll pigments, mg/ dm-2 Carotenoid pigment content, mg/ dm-2 Apical shoot growth, cm Annual shoot growth in relation to initial dimensions, % a b sum 14.05.2015 1. Control 2. Linaroside 0.01% 3.Verbascoside 0.01% - - - - - 8.0±1.7 14.0±4.3 7.0±2.7 0.00 0.00 0.00 04.06.2015 1. Control 2. Linaroside 0.01% 3.Verbascoside 0.01% 3.08±0.45 2.49± 0.45 2.32± 014 1.65±0.08 1.82±0.04 1.84±0.03 0.35±0.05 0.46 ±0.02 0.45 ±0.02 2.00±0.02 2.28±0.01 2.29±0.05 0.69±0.05 0.71±0.06 0.75±0.02 14.2±5.5 24.0±4.3 21.0±1.5 77.5±43.6 71.4±49.7 200.0±78.8 18.06.2015 1. Control 2. Linaroside 0.01% 3.Verbascoside 0.01% 1.85± 0.06 2.33 ±0.04 3.38± 0.35 2.21±0.05 2.47±0.06 2.61±0.09 0.41±0.02 0.57±0.03 0.60±0.03 2.64±0.07 3.04±0.04 3.21±0.11 0.92±0.03 1.00±0.04 1.03±0.04 14.5±6.7 26.0±4.8 23.1±1.9 81.3±51.0 85.7±46.6 230.0±97.7 02.07.2015 1. Control 2. Linaroside 0.01% 3.Verbascoside 0.01% 1.83± 0.60 1.10± 0.08 3.14± 0.08 2.25±0.01 2.40±0.08 2.72±0.05 0.54±0.06 0.55±0.01 0.75±0.03 2.79±0.01 2.95±0.03 3.47±0.00 0.88±0.07 0.80±0.03 1.09±0.04 14.5±9.4 33.8±9.9 23.3±0.6 81.3±70.6 141.4±10.5 233.0±81.1 17.07.2015 1. Control 2. Linaroside 0.01% 3.Verbascoside 0.01% 1.55 ±0.19 2.57±0.16 2.94±0.15 2.37±0.02 2.32±0.04 2.85±0.08 0.64±0.08 0.55±0.01 0.75±0.04 3.01±0.07 2.87±0.06 3.06±0.14 1.18±0.03 1.01±0.02 1.17±0.04 14.5 ±9.4 33.8±9.9 23.3 ±0.6 81.3±70.6 141.4±10.5 233.0±81.1
Din datele prezentate în tabel rezultă că utilizarea glicozidelor obţinute din Verbascum phlomoides la tratarea pomilor de păr de soiul Noiabriskaia a contribuit semnificativ asupra intensităţii proceselor de fotosinteză, de acumulare a pigmenţilor clorofilieni şi carotenoizi, precum şi asupra creşterii lăstarilor apicali pe durata perioadei de vegetaţie. Aşadar, intensitatea fotosintezei în varianta utilizării procedeului propus (invenţia) a fost cea mai sporită, alcătuind 0,37…1,05 µmol/dm-2/sec-1 comparativ cu cea mai apropiată soluţie şi cu 0,39…1,4 µmol/dm-2/sec-1 comparativ cu martorul. Rezultate similare au fost obţinute şi la determinarea conţinutului pigmenţilor carotenoizi, care a fost mai sporit faţă de cea mai apropiată soluţie cu 0,04…0,029 mg/dm2, iar faţă de varianta martorului cu 0,06…0,21 mg/dm2 . Utilizarea regulatorului de creştere propus (invenţia) a contribuit şi la intensificarea creşterii vegetative a lăstarilor apicali pe durata estimării, care a fost mai sporită faţă de cea mai apropiată soluţie cu 92,6% şi faţă de martor cu 151,7%. From the data presented in the table it results that the use of glycosides obtained from Verbascum phlomoides in the treatment of mullein trees of the Noiabriskaia variety contributed significantly to the intensity of photosynthesis processes, accumulation of chlorophyll and carotenoid pigments, as well as to the growth of apical shoots during the vegetation period. Therefore, the intensity of photosynthesis in the variant using the proposed process (invention) was the most increased, making up 0.37…1.05 µmol/dm-2/sec-1 compared to the closest solution and by 0.39…1.4 µmol/dm-2/sec-1 compared to the control. Similar results were obtained when determining the content of carotenoid pigments, which was higher compared to the closest solution by 0.04…0.029 mg/dm2, and compared to the control variant by 0.06…0.21 mg/dm2. The use of the proposed growth regulator (invention) also contributed to the intensification of the vegetative growth of the apical shoots during the estimation period, which was increased compared to the closest solution by 92.6% and compared to the control by 151.7%.
Aşadar, tratarea pomilor fructiferi cu soluţie apoasă de 0,01% de verbascozide contribuie la intensificarea proceselor de fotosinteză a pomilor de păr, de sinteză a pigmenţilor clorofilieni şi sumei lor, pigmenţilor carotenoizi, precum şi la creşterea vegetativă intensivă a lăstarilor anuali, fapt ce poate contribui în mare măsură la sporirea productivităţii pomilor de păr şi a calităţii recoltei de fructe. Therefore, treating fruit trees with an aqueous solution of 0.01% verbascosides contributes to the intensification of the photosynthesis processes of hair trees, the synthesis of chlorophyll pigments and their sum, carotenoid pigments, as well as the intensive vegetative growth of annual shoots, which can greatly contribute to increasing the productivity of hair trees and the quality of the fruit harvest.
1. Русу М.М., Балмуш Г.Т., Мащенко Н.Е. Влияние препарата Линарозид на пигментный комплекс листьев яблони. Сборник Растения в условиях глобальных и локальных природно-климатических и антропогенных воздействий, Петрозаводск, 2015, p. 459 1. Русу М.М., Балмуш Г.Т., Машченко Н.Е. The effect of Linarozid preparation on the pigment complex of apple leaves. Сборник Растения в условияных гобальных и логаных придоро-климатических и anthropogennyh vozdeystvii, Petrozavodsk, 2015, p. 459
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| MDS20150155A MD1042Z (en) | 2015-11-25 | 2015-11-25 | Process for treating young pear trees |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20150155A MD1042Z (en) | 2015-11-25 | 2015-11-25 | Process for treating young pear trees |
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| Publication Number | Publication Date |
|---|---|
| MD1042Y MD1042Y (en) | 2016-06-30 |
| MD1042Z true MD1042Z (en) | 2017-01-31 |
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| MDS20150155A MD1042Z (en) | 2015-11-25 | 2015-11-25 | Process for treating young pear trees |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD3176F1 (en) * | 2006-02-20 | 2006-11-30 | Institutul De Fiziologie A Plantelor Al Academieide Stiinte A Republicii Moldova | Process for cultivation of fruit-bearing plants |
| MD3442F1 (en) * | 2007-06-07 | 2007-12-31 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Process for apple-tree treatment |
| MD3582F1 (en) * | 2007-10-16 | 2008-05-31 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Process for apple-tree treatment |
| MD3609F1 (en) * | 2007-11-15 | 2008-06-30 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Process for apple-tree cultivation |
| MD328Y (en) * | 2010-09-28 | 2011-02-28 | Inst De Genetica Si Fiziol A Plantelor Al Academiei De Stiinte A Moldovei | Process for presowing treatment of sweet pepper seeds |
| MD862Y (en) * | 2014-08-15 | 2015-01-31 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | Process for growing tobacco plants |
| MD901Y (en) * | 2014-10-31 | 2015-05-31 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | Process for presowing treatment of cucumber seeds |
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2015
- 2015-11-25 MD MDS20150155A patent/MD1042Z/en not_active IP Right Cessation
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD3176F1 (en) * | 2006-02-20 | 2006-11-30 | Institutul De Fiziologie A Plantelor Al Academieide Stiinte A Republicii Moldova | Process for cultivation of fruit-bearing plants |
| MD3442F1 (en) * | 2007-06-07 | 2007-12-31 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Process for apple-tree treatment |
| MD3582F1 (en) * | 2007-10-16 | 2008-05-31 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Process for apple-tree treatment |
| MD3609F1 (en) * | 2007-11-15 | 2008-06-30 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Process for apple-tree cultivation |
| MD328Y (en) * | 2010-09-28 | 2011-02-28 | Inst De Genetica Si Fiziol A Plantelor Al Academiei De Stiinte A Moldovei | Process for presowing treatment of sweet pepper seeds |
| MD862Y (en) * | 2014-08-15 | 2015-01-31 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | Process for growing tobacco plants |
| MD901Y (en) * | 2014-10-31 | 2015-05-31 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | Process for presowing treatment of cucumber seeds |
Non-Patent Citations (1)
| Title |
|---|
| Русу М.М., Балмуш Г.Т., Мащенко Н.Е. Влияние препарата Линарозид на пигментный комплекс листьев яблони. Сборник Растения в условиях глобальных и локальных природно-климатических и антропогенных воздействий, Петрозаводск, 2015, p. 459 * |
Also Published As
| Publication number | Publication date |
|---|---|
| MD1042Y (en) | 2016-06-30 |
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