MD1041Z - Process for treating triticale seeds with reduced viability - Google Patents
Process for treating triticale seeds with reduced viability Download PDFInfo
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- MD1041Z MD1041Z MDS20150139A MDS20150139A MD1041Z MD 1041 Z MD1041 Z MD 1041Z MD S20150139 A MDS20150139 A MD S20150139A MD S20150139 A MDS20150139 A MD S20150139A MD 1041 Z MD1041 Z MD 1041Z
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- seeds
- treating
- triticale
- reduced viability
- viability
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- 238000000034 method Methods 0.000 title claims abstract description 13
- 230000035899 viability Effects 0.000 title claims abstract description 13
- 235000019714 Triticale Nutrition 0.000 title claims abstract description 12
- 241000228158 x Triticosecale Species 0.000 title claims abstract description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000007864 aqueous solution Substances 0.000 claims abstract description 7
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 4
- 235000014113 dietary fatty acids Nutrition 0.000 claims abstract description 4
- 239000000194 fatty acid Substances 0.000 claims abstract description 4
- 229930195729 fatty acid Natural products 0.000 claims abstract description 4
- 150000004665 fatty acids Chemical class 0.000 claims abstract description 4
- 159000000003 magnesium salts Chemical class 0.000 claims abstract description 4
- 239000011591 potassium Substances 0.000 claims abstract description 4
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 4
- 239000000377 silicon dioxide Substances 0.000 claims abstract description 4
- 235000012239 silicon dioxide Nutrition 0.000 claims abstract description 4
- 238000002791 soaking Methods 0.000 claims abstract description 3
- 239000002778 food additive Substances 0.000 claims description 4
- 235000013373 food additive Nutrition 0.000 claims description 4
- 239000013589 supplement Substances 0.000 abstract 1
- 230000035784 germination Effects 0.000 description 9
- 239000000243 solution Substances 0.000 description 6
- 230000004936 stimulating effect Effects 0.000 description 5
- 230000007774 longterm Effects 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 238000011066 ex-situ storage Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000007226 seed germination Effects 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
Invenţia se referă la agricultură, şi anume la un procedeu de tratare a seminţelor de triticale cu viabilitate redusă după păstrarea lor îndelungată în colecţiile ex situ. The invention relates to agriculture, namely to a process for treating triticale seeds with reduced viability after their long-term storage in ex situ collections.
Conservarea mostrelor de seminţe ale plantelor de cultură în Băncile de Gene se efectuează în corespundere cu standardele internaţionale care specifică procedurile de menţinere a unui nivel ridicat al viabilităţii seminţelor în colecţiile de bază sau cele active pe durata întregii perioade de prezervare. Se cere a fi menţionat şi faptul că în instituţiile de ameliorare şi cercetare din domeniile adiacente materialul semincer al mostrelor incluse în studiu se păstrează în colecţii de lucru fără posibilitatea respectării condiţiilor necesare de temperatură şi umiditate. Ca rezultat, după o perioadă scurtă de timp, are loc diminuarea viabilităţii lor, ceea ce impune necesitatea aplicării diferitor metode de sporire a germinaţiei seminţelor. Preservation of seed samples of cultivated plants in Gene Banks is carried out in accordance with international standards that specify procedures for maintaining a high level of seed viability in basic or active collections throughout the entire preservation period. It should also be noted that in breeding and research institutions in adjacent areas the seed material of the samples included in the study is kept in working collections without the possibility of observing the necessary temperature and humidity conditions. As a result, after a short period of time, their viability decreases, which requires the application of various methods of increasing seed germination.
Este cunoscut procedeul de sporire a viabilităţii seminţelor de triticale după păstrarea lor îndelungată prin tratarea cu soluţie apoasă de tomatozidă [1]. The process of increasing the viability of triticale seeds after their long-term storage by treating them with an aqueous solution of tomatoside is known [1].
Unul din neajunsurile procedeului dat poate fi considerat faptul că pentru obţinerea efectului stimulativ maximal privind mărirea viabilităţii seminţelor de triticale se cere utilizarea unor concentraţii mai mari de tomatozidă (de zeci de ori) comparativ cu compusul chimic din procedeul propus. One of the shortcomings of this process can be considered the fact that to obtain the maximum stimulating effect on increasing the viability of triticale seeds, it is required to use higher concentrations of tomatozide (tens of times) compared to the chemical compound in the proposed process.
Problema pe care o rezolvă invenţia constă în mărirea viabilităţii seminţelor de triticale cu germinaţie redusă după păstrarea lor îndelungată ex situ în colecţiile de lucru. The problem solved by the invention consists in increasing the viability of triticale seeds with reduced germination after their long-term storage ex situ in working collections.
Invenţia soluţionează problema prin aceea că se propune un procedeu de tratare a seminţelor de triticale cu viabilitate redusă care constă în înmuierea seminţelor într-o soluţie apoasă de 0,00005…0,1% a unui adaos alimentar biologic activ, care conţine dioxid de siliciu şi cantităţi mici de acizi graşi, săruri de potasiu şi magneziu, timp de 18 ore. The invention solves the problem by proposing a process for treating triticale seeds with reduced viability, which consists of soaking the seeds in an aqueous solution of 0.00005…0.1% of a biologically active food additive, which contains silicon dioxide and small amounts of fatty acids, potassium and magnesium salts, for 18 hours.
Adaosul alimentar biologic activ este cunoscut sub denumirea de microhidrină produs de firma Royal Body Care (Микрогидрин. Сборник статей. Москва, 1999, 27 p.; Данилова М.А., Гольберг Н.Д., Петров М.Г. и др. Эффективность применения антиоксидантов при физических нагрузках. Сборник трудов Санкт-Петербургского научно-исследовательского института физической культуры. Итоговая научная конференция, 16-19 декабря 2006, p.196-200). The biologically active food additive is known as microhydrin produced by the company Royal Body Care (Микрогидрин. Сборник стаей. Москва, 1999, 27 p.; Danilova М.А., Гольберг Н.Д., Петров М.Г. и др. Эффективность применения отикандистов при физических нагрузках. Сборник трудов Санкт-Петербургского научно-иследванеского института физической культуры. Итоговая научания конференция, 16-19 декабря 2006, p.196-200).
Rezultatul invenţiei constă în sporirea viabilităţii seminţelor de triticale după păstrarea lor îndelungată ex situ în colecţiile de lucru. The result of the invention consists in increasing the viability of triticale seeds after their long-term ex situ storage in working collections.
Exemplu de realizare a invenţiei Example of embodiment of the invention
Seminţe îmbătrânite de triticale de soiul Ingen 93 (perioada de păstrare 10 ani) se înmoaie într-o soluţie apoasă de 0,00005…0,1% a unui adaos alimentar biologic activ, care conţine dioxid de siliciu şi cantităţi mici de acizi graşi, săruri de potasiu şi magneziu, timp de 18 ore, la temperatura de 20°С (invenţia propusă). Aged seeds of triticale of the Ingen 93 variety (storage period 10 years) are soaked in a 0.00005…0.1% aqueous solution of a biologically active food additive, which contains silicon dioxide and small amounts of fatty acids, potassium and magnesium salts, for 18 hours at a temperature of 20°С (proposed invention).
Pentru obţinerea unor rezultate comparative seminţele au fost tratate cu soluţie apoasă de tomatozidă în concentraţie de 0,01% timp de 18 ore (cea mai apropiată soluţie). Ca martor au servit seminţele umectate în apă distilată (18 ore). După 18 ore de aplicare a soluţiilor seminţele au fost clătite cu apă şi amplasate în cutii Petri pe hârtie de filtru umectată cu apă distilată. În fiecare variantă a experienţei s-au utilizat 4 cutii Petri cu câte 100 de seminţe. Cutiile au fost amplasate în termostat pentru germinare la temperatura de 20°С. Energia germinativă a fost determinată la a 4-a zi, iar germinaţia finală - la a 8-a zi. Rezultatele sunt prezentate în tabel. To obtain comparative results, the seeds were treated with an aqueous solution of tomatozide in a concentration of 0.01% for 18 hours (the closest solution). Seeds moistened in distilled water (18 hours) served as a control. After 18 hours of application of the solutions, the seeds were rinsed with water and placed in Petri dishes on filter paper moistened with distilled water. In each variant of the experiment, 4 Petri dishes with 100 seeds each were used. The boxes were placed in a thermostat for germination at a temperature of 20°С. Germination energy was determined on the 4th day, and final germination - on the 8th day. The results are presented in the table.
Tabel Table
№ variantei Varianta Concentraţia, % Energia germinativă, % Germinaţia, % Lungimea rădăciniţelor, mm 1 microhidrină 0,00005 65,4±4,8** 82,6±5,2** 14,2±1,4 2 “ - “ 0,0001 68,1±3,8** 88,9±3,6*** 15,0±1,1 3 “ - “ 0,0005 55,5±2,8* 78,0±3,3** 15,4±1,6 4 „ - „ 0,001 56,3±3,1* 78,8±2,9** 14,9±0,8 5 „ - „ 0,005 54,0±3,8 78,6±2,1** 13,8±1,2 6 “ - “ 0,01 55,3±3,1 74,8±3,0* 13,5±1,6 7 “ - “ 0,05 50,7±5,1 72,4±3,1 14,5±1,2 8 “ - “ 0,1 45,2±3,0 64,0±2,5* 13,1±1,4 9 tomatozidă 0,01 45,4±4,8 64,5±2,8 12,9±0,8 10 martor - 40,7±4,8 63,8±3,8 12,5±0,7Variant No. Variant Concentration, % Germination energy, % Germination, % Root length, mm 1 microhydrin 0.00005 65.4±4.8** 82.6±5.2** 14.2±1.4 2 “ - “ 0.0001 68.1±3.8** 88.9±3.6*** 15.0±1.1 3 “ - “ 0.0005 55.5±2.8* 78.0±3.3** 15.4±1.6 4 “ - “ 0.001 56.3±3.1* 78.8±2.9** 14.9±0.8 5 “ - “ 0.005 54.0±3.8 78.6±2.1** 13.8±1.2 6 “ - “ 0.01 55.3±3.1 74.8±3.0* 13.5±1.6 7 “ - “ 0.05 50.7±5.1 72.4±3.1 14.5±1.2 8 “ - “ 0.1 45.2±3.0 64.0±2.5* 13.1±1.4 9 tomatoside 0.01 45.4±4.8 64.5±2.8 12.9±0.8 10 control - 40.7±4.8 63.8±3.8 12.5±0.7
*, **, *** - Diferenţe semnificative faţă de martor la P<0,05 ; P<0,01; P<0,001 *, **, *** - Significant differences compared to control at P<0.05; P<0.01; P<0.001
După cum rezultă din tabelul prezentat, acţiune stimulatoare s-a observat la utilizarea tuturor concentraţiilor studiate ale microhidrinei - de la 0,00005% până la 0,1%. Efectul stimulator maximal s-a evidenţiat în cazul prelucrării seminţelor cu microhidrină în concentraţie de 0,0001%. Energia germinativă a seminţelor a crescut comparativ cu martorul cu 27,4%, iar faţă de cea mai apropiată soluţie - cu 22,7%. Germinaţia seminţelor de triticale a fost superioară martorului cu 25,1%, iar faţă de cea mai apropiată soluţie - cu 24,4%. Lungimea rădăciniţelor în varianta stimulatoare (concentraţia microhidrinei de 0,0001%) faţă de martor a fost mai mare cu 2,5 mm şi cu 2,1 mm comparativ cu cea mai apropiată soluţie. As can be seen from the table presented, stimulating action was observed when using all studied concentrations of microhydrin - from 0.00005% to 0.1%. The maximum stimulating effect was evident in the case of processing seeds with microhydrin in a concentration of 0.0001%. The germination energy of seeds increased compared to the control by 27.4%, and compared to the closest solution - by 22.7%. The germination of triticale seeds was superior to the control by 25.1%, and compared to the closest solution - by 24.4%. The length of roots in the stimulating variant (microhydrin concentration of 0.0001%) compared to the control was greater by 2.5 mm and by 2.1 mm compared to the closest solution.
Este necesar de menţionat faptul că concentraţia stimulativă maximală a microhidrinei depistată este la două ordine de mărime mai mică decât la cea mai apropiată soluţie (tomatozidă), cea ce este mai avantajos din punct de vedere al eficienţei şi rentabilităţii procedeului. It is necessary to mention that the maximum stimulating concentration of the detected microhydrin is two orders of magnitude lower than the closest solution (tomatoside), which is more advantageous from the point of view of the efficiency and profitability of the process.
Astfel, procedeul de tratare înainte de semănat a seminţelor îmbătrânite de triticale cu viabilitate redusă cu soluţii apoase de microhidrină în concentraţii de 0,00005…0,1% contribuie la majorarea substanţială a energiei germinative şi a germinaţiei seminţelor, în final la mărirea viabilităţii lor. Thus, the pre-sowing treatment process of aged triticale seeds with reduced viability with aqueous solutions of microhydrin in concentrations of 0.00005…0.1% contributes to a substantial increase in seed germination energy and germination, ultimately increasing their viability.
1. MD 154 Y 2010.03.31 1. MD 154 Y 2010.03.31
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20150139A MD1041Z (en) | 2015-10-16 | 2015-10-16 | Process for treating triticale seeds with reduced viability |
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| MDS20150139A MD1041Z (en) | 2015-10-16 | 2015-10-16 | Process for treating triticale seeds with reduced viability |
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| MD1041Y MD1041Y (en) | 2016-06-30 |
| MD1041Z true MD1041Z (en) | 2017-01-31 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD1800F1 (en) * | 2000-06-02 | 2001-12-31 | Inst Genetica Rep Moldova | Triticale plant growth stimulator |
| MD20010274A (en) * | 2001-08-24 | 2003-06-30 | Inst Genetica Rep Moldova | Process for obtaining of hybrid seeds in distant hybridization |
| MD154Y (en) * | 2009-07-01 | 2010-03-31 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Process for treating triticale seeds with reduced viability |
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- 2015-10-16 MD MDS20150139A patent/MD1041Z/en not_active IP Right Cessation
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD1800F1 (en) * | 2000-06-02 | 2001-12-31 | Inst Genetica Rep Moldova | Triticale plant growth stimulator |
| MD20010274A (en) * | 2001-08-24 | 2003-06-30 | Inst Genetica Rep Moldova | Process for obtaining of hybrid seeds in distant hybridization |
| MD154Y (en) * | 2009-07-01 | 2010-03-31 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Process for treating triticale seeds with reduced viability |
Non-Patent Citations (2)
| Title |
|---|
| Данилова М. А., Гольберг Н.Д., Петров М.Г. и др. Эффективность применения антиоксидантов при физических нагрузках. Сборник трудв Санкт-Петербурского научно-исследовательского института физической культуры. Итоговая научная конференция. 16-19 декабря 2006 года, р. 196-200 * |
| Микрогидрин. Сборник статей. Москва, 1999, 27 р. * |
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| MD1041Y (en) | 2016-06-30 |
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