MD1237Y - Process for presowing treatment of cereal seeds - Google Patents
Process for presowing treatment of cereal seeds Download PDFInfo
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- MD1237Y MD1237Y MDS20170092A MDS20170092A MD1237Y MD 1237 Y MD1237 Y MD 1237Y MD S20170092 A MDS20170092 A MD S20170092A MD S20170092 A MDS20170092 A MD S20170092A MD 1237 Y MD1237 Y MD 1237Y
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- seeds
- culture liquid
- cnmn
- water
- wheat
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- 238000000034 method Methods 0.000 title claims abstract description 14
- 235000013339 cereals Nutrition 0.000 title claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 17
- 241000209140 Triticum Species 0.000 claims abstract description 16
- 235000021307 Triticum Nutrition 0.000 claims abstract description 16
- 241000864266 Penicillium verrucosum Species 0.000 claims abstract description 15
- 239000000203 mixture Substances 0.000 claims abstract description 14
- 235000019714 Triticale Nutrition 0.000 claims abstract description 12
- 241000228158 x Triticosecale Species 0.000 claims abstract description 12
- 238000002791 soaking Methods 0.000 claims abstract description 6
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 6
- 238000009331 sowing Methods 0.000 claims description 6
- 239000002028 Biomass Substances 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 235000010344 sodium nitrate Nutrition 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 230000035784 germination Effects 0.000 description 15
- 239000000243 solution Substances 0.000 description 12
- 239000002207 metabolite Substances 0.000 description 7
- 230000004936 stimulating effect Effects 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 3
- 230000035800 maturation Effects 0.000 description 3
- 230000007226 seed germination Effects 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000134403 Mortierella polycephala Species 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
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- Pretreatment Of Seeds And Plants (AREA)
Abstract
Description
Invenţia se referă la agricultură, şi anume la un procedeu de tratare a seminţelor de cereale înainte de semănat. The invention relates to agriculture, namely to a process for treating cereal seeds before sowing.
În prezent sunt cunoscute diverse procedee de tratare a seminţelor culturilor de graminee în scopul sporirii energiei germinative şi a germinării acestora. Currently, various processes are known for treating grass seed crops in order to increase their germination energy and germination.
Este cunoscut procedeul de tratare a seminţelor de grâu înainte de semănat cu o soluţie de biopreparat obţinut din biomasa micromicetelor din genul Penicillium [1]. The process of treating wheat seeds before sowing with a solution of a biopreparation obtained from the biomass of micromycetes of the genus Penicillium is known [1].
Mai este cunoscut procedeul de tratare a seminţelor de grâu înainte de semănat cu o soluţie a biopreparatului obţinut pe baza biomasei tulpinii fungice Mortierella polycephala, care are acţiune împotriva fitopatogenilor şi sporeşte productivitatea plantelor [2]. The process of treating wheat seeds before sowing with a solution of the biopreparation obtained based on the biomass of the fungal strain Mortierella polycephala, which has action against phytopathogens and increases plant productivity, is also known [2].
Dezavantajul acestor procedee constă în efortul şi cheltuielile mari necesare atât pentru obţinerea preparatelor, cât şi pentru utilizarea lor. The disadvantage of these processes lies in the high effort and expense required both for obtaining the preparations and for their use.
În calitate de cea mai apropiată soluţie poate servi procedeul de tratare a seminţelor de grâu (soiul «Зустрич») cu soluţia biopreparatului „Байкал-1-У” (concentraţia 1:1000, timp de 4 ore) [3]. The closest solution may be the treatment of wheat seeds (variety "Zustrich") with a solution of the biopreparation "Baikal-1-U" (concentration 1:1000, for 4 hours) [3].
Dezavantajul acestui procedeu constă în lucrul anevoios necesar pentru obţinerea biopreparatului care constă din mai multe grupuri de microorganisme (bacterii lactice, azotfixatoare, fotosintetice, drojdii (aerobe şi anaerobe)). The disadvantage of this process is the laborious work required to obtain the biopreparation, which consists of several groups of microorganisms (lactic acid bacteria, nitrogen-fixing bacteria, photosynthetic bacteria, yeasts (aerobic and anaerobic)).
Problema tehnică pe care o rezolvă prezenta invenţie constă în elaborarea unui procedeu de tratare a seminţelor de grâu şi triticale, înainte de semănat, cu o soluţie de metaboliţi, care să asigure stimularea energiei germinative şi a germinării seminţelor, pentru a accelera maturarea culturilor şi productivitatea lor. The technical problem solved by the present invention consists in developing a process for treating wheat and triticale seeds, before sowing, with a solution of metabolites, which ensures the stimulation of germinative energy and seed germination, in order to accelerate crop maturation and their productivity.
Invenţia soluţionează problema prin aceea că se propune un procedeu de tratare a seminţelor de cereale înainte de semănat, care include înmuierea acestora într-un amestec de lichid cultural al tulpinii Penicillium verrucosum CNMN-FD-19 şi apă, luate în raport respectiv de 1:300, lichidul cultural fiind obţinut la cultivarea submersă a tulpinii pe un mediu nutritiv care conţine, în %: glucoză 4,0, NaNO3 2,0, K2HPO4 2,5, MgSO4x7H2O 1,0, FeSO4x7H2O 0,01, extract de drojdii 1,5 şi apă distilată restul, la temperatura de 28…30°C, timp de 6 zile cu agitare continuă şi separarea ulterioară a biomasei, totodată înmuierea se efectuează în decurs de 2 ore, cu o normă de consum de 0,1 L/kg seminţe, iar în calitate de seminţe de cereale se utilizează seminţele de grâu sau triticale. The invention solves the problem by proposing a process for treating cereal seeds before sowing, which includes soaking them in a mixture of culture liquid of the Penicillium verrucosum CNMN-FD-19 strain and water, taken in a respective ratio of 1:300, the culture liquid being obtained from submerged cultivation of the strain on a nutrient medium containing, in %: glucose 4.0, NaNO3 2.0, K2HPO4 2.5, MgSO4x7H2O 1.0, FeSO4x7H2O 0.01, yeast extract 1.5 and distilled water the rest, at a temperature of 28…30°C, for 6 days with continuous stirring and subsequent separation of the biomass, at the same time soaking is carried out within 2 hours, with a consumption rate of 0.1 L/kg of seeds, and as cereal seeds are used seeds of wheat or triticale.
Rezultatul tehnic constă în stimularea energiei germinative şi a germinării seminţelor de grâu şi triticale, care contribuie ulterior la accelerarea începutului maturării culturilor, precum şi la stimularea productivităţii lor. The technical result consists in stimulating the germinative energy and germination of wheat and triticale seeds, which subsequently contributes to accelerating the beginning of crop maturation, as well as to stimulating their productivity.
Datele prezentate reprezintă media a 10 probe. The data presented represent the average of 10 samples.
Exemplu de realizare a invenţiei Example of embodiment of the invention
Pentru obţinerea metaboliţilor tulpina Penicillium verrucosum CNMN-FD-19 se cultivă în baloane Erlenmayer de 0,75 L în care se introduc câte 200 ml mediu cu compoziţia (%): glucoză 4,0; NaNO3 2,0; K2HPO4 2,5; MgSO4x7H2O 1,0; FeSO4x7H2O 0,01; extract de drojdii 1,5; pH 6,2, apă distilată până la 1 litru, în condiţii de agitare continuă (160…180 r.p.m.) la temperatura de 28…30°C timp de 6 zile. Prin filtrare se separă lichidul cultural de biomasă. Lichidul cultural (metaboliţii) a fost diluat în proporţie de 1:100; 1:200; 1:300; 1:400; 1:500. To obtain metabolites, the Penicillium verrucosum strain CNMN-FD-19 is cultivated in 0.75 L Erlenmayer flasks, in which 200 ml of medium with the composition (%) are introduced: glucose 4.0; NaNO3 2.0; K2HPO4 2.5; MgSO4x7H2O 1.0; FeSO4x7H2O 0.01; yeast extract 1.5; pH 6.2, distilled water up to 1 liter, under continuous stirring conditions (160…180 r.p.m.) at a temperature of 28…30°C for 6 days. The culture liquid is separated from the biomass by filtration. The culture liquid (metabolites) was diluted in a ratio of 1:100; 1:200; 1:300; 1:400; 1:500.
În calitate de material semincer s-au folosit seminţe de grâu soiul „Arnautca” şi triticale soiul „Ingen 93”. Seminţele de grâu şi triticale au fost înmuiate în suspensia apoasă de metaboliţi cu concentraţiile menţionate, timp de 2 ore (norma de consum 0,1 L/kg seminţe), apoi au fost amplasate în cutii Petri pe hârtie de filtru umectată cu apă distilată. Cutiile au fost amplasate în termostat pentru germinare la tempetratura de 24°C, timp de 7 zile. La a 3-a zi de cultivare a fost deterinată energia germinativă, iar la a 7-a zi germinarea finală a seminţelor. Ca martor au servit seminţele umectate în apă distilată. As seed material, wheat seeds of the “Arnautca” variety and triticale seeds of the “Ingen 93” variety were used. Wheat and triticale seeds were soaked in the aqueous suspension of metabolites with the mentioned concentrations for 2 hours (consumption rate 0.1 L/kg seeds), then they were placed in Petri dishes on filter paper moistened with distilled water. The boxes were placed in a thermostat for germination at a temperature of 24°C for 7 days. On the 3rd day of cultivation, the germination energy was determined, and on the 7th day, the final germination of the seeds. Seeds moistened in distilled water served as a control.
Determinarea energiei germinative şi a germinării seminţelor s-a efectuat conform metodei descrise în ГОСТ 12038-84 «Семена сельскохозяйственных культур. Методы определения всхожести». The determination of seed germination energy and germination was carried out according to the method described in GOST 12038-84 "Seeds of agricultural crops. Methods for determining germination".
Rezultatele sunt prezentate în tabel. The results are presented in the table.
Tabel Table
Preparatul, concentraţia Cultura Energia germinativă (%) Germinarea seminţelor (%) Soluţia proximă, biopreparatul „Байкал - 1 - У” (1:1000) Grâu, soiul „Зустрич” 82 88 Amestec de lichid cultural de Penicillium verrucosum CNMN-FD-19 şi apă (1:100) Grâu, soiul „Arnautca” 84 86 Amestec de lichid cultural de Penicillium verrucosum CNMN-FD- 19 şi apă (1:200) - 86 90 Amestec de lichid cultural de Penicillium verrucosum CNMN-FD-19 şi apă (1:300) - 92 96 Amestec de lichid cultural de Penicillium verrucosum CNMN-FD-19şi apă (1:400) - 88 92 Amestec de lichid cultural de Penicillium verrucosum CNMN-FD-19 şi apă (1:500) - 82 86 H2O (martor) - 76 82 Amestec de lichid cultural de Penicillium verrucosum CNMN-FD-19 şi apă (1:100) Triticale soiul „Ingen 93” 75 77 Amestec de lichid cultural de Penicillium verrucosum CNMN-FD-19 şi apă (1:200) - 85 87 Amestec de lichid cultural de Penicillium verrucosum CNMN-FD-19 şi apă (1:300) - 92 96 Amestec de lichid cultural de Penicillium verrucosum CNMN-FD-19 şi apă (1:400) - 88 93 Amestec de lichid cultural de Penicillium verrucosum CNMN-FD-19 şi apă (1:500) - 85 89 H2O (martor) - 78 85Preparation, concentration Culture Germination energy (%) Seed germination (%) Proximate solution, biopreparation “Байкал - 1 - У” (1:1000) Wheat, variety “Зустрич” 82 88 Mixture of culture liquid of Penicillium verrucosum CNMN-FD-19 and water (1:100) Wheat, variety “Arnautca” 84 86 Mixture of culture liquid of Penicillium verrucosum CNMN-FD- 19 and water (1:200) - 86 90 Mixture of culture liquid of Penicillium verrucosum CNMN-FD-19 and water (1:300) - 92 96 Mixture of culture liquid of Penicillium verrucosum CNMN-FD-19 and water (1:400) - 88 92 Mixture of culture liquid of Penicillium verrucosum CNMN-FD-19 and water (1:500) - 82 86 H2O (control) - 76 82 Mixture of Penicillium verrucosum CNMN-FD-19 culture liquid and water (1:100) Triticale variety "Ingen 93" 75 77 Mixture of Penicillium verrucosum CNMN-FD-19 culture liquid and water (1:200) - 85 87 Mixture of Penicillium verrucosum CNMN-FD-19 culture liquid and water (1:300) - 92 96 Mixture of Penicillium verrucosum CNMN-FD-19 culture liquid and water (1:400) - 88 93 Mixture of Penicillium verrucosum CNMN-FD-19 culture liquid and water (1:500) - 85 89 H2O (control) - 78 85
După cum rezultă din tabelul prezentat, acţiune stimulatoare s-a observat atât la grâu, cât şi la triticale, la utilizarea tuturor concentraţiilor de metaboliţi de la 0,002% până la 0,01%. Efectul stimulator maximal, atât la grâu, cât şi la triticale, s-a evidenţiat în cazul prelucrării seminţelor cu soluţie de metaboliţi în concentraţie de 0,003%. Energia germinativă a seminţelor de grâu a crescut cu 10% faţă de soluţia proximă şi cu 16% faţă de martor, iar germinarea finală cu 8% faţă de soluţia proximă şi cu 13% faţă de martor. În cazul seminţelor de triticale, energia germinativă s-a majorat cu 10% faţă de soluţia proximă şi cu 14% faţă de martor, iar germinarea finală cu 8% faţă de soluţia proximă şi cu 11% faţă de martor. As can be seen from the table presented, a stimulating action was observed in both wheat and triticale, when using all metabolite concentrations from 0.002% to 0.01%. The maximum stimulating effect, in both wheat and triticale, was evident in the case of processing seeds with metabolite solution in a concentration of 0.003%. The germination energy of wheat seeds increased by 10% compared to the proximal solution and by 16% compared to the control, and the final germination by 8% compared to the proximal solution and by 13% compared to the control. In the case of triticale seeds, the germination energy increased by 10% compared to the proximal solution and by 14% compared to the control, and the final germination by 8% compared to the proximal solution and by 11% compared to the control.
Rezultatele prezentate demonstrează că tratarea seminţelor cu soluţia apoasă de metaboliţi ai tulpinii Penicillium verrucosum CNMN-FD-19 este mai eficientă şi mai rentabilă decât cea mai apropiată soluţie, contribuie la stimularea energiei de germinare şi a germinării culturilor de grâu şi triticale, ceea ce duce la accelerarea maturării plantelor şi la stimularea productivităţii lor. The presented results demonstrate that treating seeds with the aqueous solution of metabolites of the Penicillium verrucosum strain CNMN-FD-19 is more efficient and cost-effective than the closest solution, contributes to stimulating the germination energy and germination of wheat and triticale crops, which leads to accelerated plant maturation and stimulation of their productivity.
1. RU 2302731 C1 2007.07.20 1. RU 2302731 C1 2007.07.20
2. RU 2341947 C1 2008.12.27 2. RU 2341947 C1 2008.12.27
3. Бурыкина С.И., Коваленко Е.В., Иванов Г. И., Васильев Г.С. Применение препарата "Байкал ЭМ1" на Украине. Găsit Internet, URL: <http://emcooperation.ru/primenenie_preparata_baykal_em1_n> 3. Burykina S.I., Kovalenko E.V., Ivanov G. I., Vasiliev G.S. Application of the preparation "Baikal ЕМ1" in Ukraine. Found Internet, URL: <http://emcooperation.ru/primenenie_preparata_baykal_em1_n>
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD1659F1 (en) * | 1999-08-05 | 2001-05-31 | Inst De Fiziol A Plantelor Al | Process for presawing treatment of winter wheat seeds |
| MD1800F1 (en) * | 2000-06-02 | 2001-12-31 | Inst Genetica Rep Moldova | Triticale plant growth stimulator |
| RU2302731C1 (en) * | 2005-11-09 | 2007-07-20 | Федеральное государственное образовательное учреждение высшего профессионального образования Ставропольский государственный аграрный университет | Method for production of wheat growth regulator |
| MD3487F1 (en) * | 2007-07-11 | 2008-01-31 | Institutul De Microbiologie Si Biotehnologie Al Academiei De Stiinte A Moldovei | Stem Penicillium verrucosum Dierckx var.cyclopium - Metalaxyl and benzothiazole destroyers |
| RU2341947C1 (en) * | 2007-06-26 | 2008-12-27 | Олег Иванович Квасенков | Method of preplant seeds treatment |
| MD154Y (en) * | 2009-07-01 | 2010-03-31 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Process for treating triticale seeds with reduced viability |
| MD779Y (en) * | 2013-11-08 | 2014-06-30 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | Process for pre-sowing treatment of soft winter wheat |
| MD819Y (en) * | 2014-03-07 | 2014-10-31 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | Process for treating winter wheat against Fusarium oxysporum |
| EA021327B1 (en) * | 2008-07-09 | 2015-05-29 | Байер Кропсайенс Аг | Plant growth regulator |
| MD902Y (en) * | 2014-09-03 | 2015-05-31 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | Process for treatment of spring wheat |
-
2017
- 2017-08-10 MD MDS20170092A patent/MD1237Z/en not_active IP Right Cessation
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD1659F1 (en) * | 1999-08-05 | 2001-05-31 | Inst De Fiziol A Plantelor Al | Process for presawing treatment of winter wheat seeds |
| MD1800F1 (en) * | 2000-06-02 | 2001-12-31 | Inst Genetica Rep Moldova | Triticale plant growth stimulator |
| RU2302731C1 (en) * | 2005-11-09 | 2007-07-20 | Федеральное государственное образовательное учреждение высшего профессионального образования Ставропольский государственный аграрный университет | Method for production of wheat growth regulator |
| RU2341947C1 (en) * | 2007-06-26 | 2008-12-27 | Олег Иванович Квасенков | Method of preplant seeds treatment |
| MD3487F1 (en) * | 2007-07-11 | 2008-01-31 | Institutul De Microbiologie Si Biotehnologie Al Academiei De Stiinte A Moldovei | Stem Penicillium verrucosum Dierckx var.cyclopium - Metalaxyl and benzothiazole destroyers |
| EA021327B1 (en) * | 2008-07-09 | 2015-05-29 | Байер Кропсайенс Аг | Plant growth regulator |
| MD154Y (en) * | 2009-07-01 | 2010-03-31 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Process for treating triticale seeds with reduced viability |
| MD779Y (en) * | 2013-11-08 | 2014-06-30 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | Process for pre-sowing treatment of soft winter wheat |
| MD819Y (en) * | 2014-03-07 | 2014-10-31 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | Process for treating winter wheat against Fusarium oxysporum |
| MD902Y (en) * | 2014-09-03 | 2015-05-31 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | Process for treatment of spring wheat |
Non-Patent Citations (1)
| Title |
|---|
| Бурыкина С.И., Коваленко Е.В., Иванов Г. И., Васильев Г.С. Применение препарата "Байкал ЭМ1" на Украине. Găsit Internet, URL: <http://emcooperation.ru/primenenie_preparata_baykal_em1_n> * |
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| Publication number | Publication date |
|---|---|
| MD1237Z (en) | 2018-10-31 |
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