LV11486B - Dna sequencing enzymes - Google Patents
Dna sequencing enzymes Download PDFInfo
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- LV11486B LV11486B LVP-96-23A LV960023A LV11486B LV 11486 B LV11486 B LV 11486B LV 960023 A LV960023 A LV 960023A LV 11486 B LV11486 B LV 11486B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (31)
- FERMENTI DNS SEKVENĒŠANAI Patenta formula 1. B saimes modificēta DNS polimerāze, kas atšķiras ar to, ka tai nav 3'-*5' eksonukleāzes aktivitātes; minētā polimerāze ir izvēlēta no grupas, kura ietver: modificēto T4 DNS polimerāzi, modificēto E.coli DNS polimerāzi II, modificēto T2 DNS polimerāzi un modificēto T6 DNS polimerāzi.
- 2. B saimes modificētā DNS polimerāze saskaņā ar 1.punktu, kur minētā modificētā T4 DNS polimerāze atšķiras ar to, ka izoleicīns kodona pozīcijā 50 ir aizvietots ar leicīnu.
- 3. B saimes modificētā DNS polimerāze saskaņā ar 1.punktu, kur minētā modificētā T4 DNS polimerāze atšķiras ar to, ka glutamīnskābe kodona pozīcijā 82 ir aizvietota ar asparagīnskābi.
- 4. B saimes modificētā DNS polimerāze saskaņā ar 1.punktu, kur minētā modificētā T4 DNS polimerāze atšķiras ar to, ka triptofāns kodona pozīcijā 213 ir aizvietots ar serīnu.
- 5. B saimes modificētā DNS polimerāze saskaņā ar 1.punktu, kur minētā T4 DNS polimerāze atšķiras ar to, ka glutamīnskābe kodona pozīcijā 255 ir aizvietota ar serīnu. - 2 -
- 6. B saimes modificētā DNS polimerāze saskaņā ar 1.punktu, kur minētā modificētā T4 DNS polimerāze atšķiras ar to, ka izoleicīns kodona pozīcijā 417 ir aizvietots ar vaiīnu.
- 7. B saimes modificētā DNS polimerāze saskaņā ar 1.punktu, kur minētā modificētā T4 DNS polimerāze atšķiras ar to, ka alanīns kodona pozīcija 737 ir aizvietots ar vaiīnu.
- 8. B saimes modificētā DNS polimerāze saskaņā ar 1.punktu, kur minētā modificētā T4 DNS polimerāze atšķiras ar to, ka alanīns kodona pozīcijā 743 ir aizvietots ar vaiīnu.
- 9. B saimes modificētā DNS polimerāze saskaņā ar 1.punktu, kur minētā modificētā T4 DNS polimerāze atšķiras ar to, ka asparagīnskābe kodona pozīcijā 112 ir aizvietota ar alanīnu.
- 10. B saimes modificētā DNS polimerāze saskaņā ar 1.punktu, kur minētā modificētā T4 DNS polimerāze atšķiras ar to, ka izoleicīns kodona pozīcijā 114 ir aizvietots ar leicīnu.
- 11. B saimes modificētā DNS polimerāze saskaņā ar 1.punktu, kur minētā modificētā T4 DNS polimerāze atšķiras ar to, ka asparagīnskābe kodona pozīcijā 219 ir aizvietota ar alanīnu.
- 12. B saimes modificētā DNS polimerāze saskaņā ar 1. punktu, kur minētā modificētā T4 DNS polimerāze atšķiras ar to, ka asparagīnskābe kodona pozīcijā 324 ir aizvietota ar alanīnu.
- 13. B saimes modificētā DNS polimerāze saskaņā ar 1.punktu, kur minētā modificētā E.coli DNS polimerāze atšķiras ar to, ka asparagīnskābe kodona pozīcijā 156 ir aizvietota ar alanīnu.
- 14. B saimes modificētā DNS polimerāze saskaņā ar 1.punktu, kur minētā modificētā E.coli DNS polimerāze II atšķiras ar to, ka glutamīnskābe kodona pozīcijā 158 ir aizvietota ar alanīnu. - 3 - - 3 - LV 11486
- 15. DNS sekvenēšanas paņēmiens, kurā no modificēto polimerāžu grupas - T4 polimerāze, E.coli DNS polimerāze, T2 polimerāze un T6 polimerāze, izvēlētu polimerāzi kontaktē ar sekvenējamo DNS praimera pavedienu dATF, dGTF, dCTF, dTTF klātienē un izvēlētiem ķēdi terminējošiem nukleotīdiem: ar pirmo no ddATF un 3'-amīno-2',3' dideoksi-ATF grupas, - otro no ddGTF un S'-amīno^'^' dideoksi-GTF grupas, - trešo no ara CTF un S'-amīno-^.S1 dideoksi-CTF grupas un - ceturto no ara UTF un 3'- amīno-2', 3' dideoksi-TTF grupas, un nodrošina kontaktu reakcijas apstākļos, lai saglabātu polimerāzes aktivitāti pietiekami ilgu laiku sekvenēšanas informācijas iegūšanai.
- 16. Paņēmiens saskaņā ar 15.punktu, kur minētais pirmais ķēdi terminējošais nukleotīds ir ddATF, minētais otrais ķēdi terminējošais nukleotīds ir ddGTF, minētais trešais ķēdi terminējošais nukleotīds ir araCTF un minētais ceturtais ķēdi terminējošais nukleotīds ir arallTF.
- 17. Paņēmiens saskaņā ar 15.punktu, kur polimerāze ir modificētā T4 polimerāze.
- 18. Paņēmiens saskaņā ar 17.punktu, kas turklāt ietver vismaz vienu papildu proteīnu, kurš veido kompleksu ar minēto modificēto T4 polimerāzi, tādējādi intensificējot minētās modificētās T4 polimerāzes procesēšanu.
- 19. Paņēmiens saskaņā ar 18.punktu, kur papildu proteīns ir izvēlēts no grupas, kas ietver T gēna produktus 32, 41,45 un 44/62 kompleksu.
- 20. Paņēmiens saskaņā ar 15.punktu, kur modificētā polimerāze ir modificētā E.coli polimerāze II.
- 21. Paņēmiens saskaņā ar 20.punktu, kas turklāt ietver vismaz vienu papildu proteīnu, kurš veido kompleksu ar modificēto E.coli DNS polimerāzi II, tādējādi intensificējot minētās modificētās E.coli DNS polimerāzes II procesēšanu.
- 22. Paņēmiens saskaņā ar 21.punktu, kur minētais papildu proteīns ir β proteīna, γ kompleksa un vienpavediena nukleīnskābes piesaistošā proteīna (SSB-single stranded binding protein) kombinācija. - 4 -
- 23. Paņēmiens saskaņā ar 15.punktu, kur minētais pirmais ķēdi terminējošais nukleotīds ir 3'-amīno-2',3' dideoksi-ATF, minētais otrais ķēdi terminējošais nukleotīds ir 3'-amīno-2',3‘ dideoksi-GTF, minētais trešais ķēdi terminējošais nukleotīds ir 3'-amīno-2',3' dideoksi-CTF un minētais ceturtais ķēdi terminējošais nukleotīds ir 3'-amīno-2',3' dideoksi-TTF.
- 24. Paņēmiens saskaņā ar 23. punktu, kur polimerāze ir izvēlēta no grupas, kas ietver T4 polimerāzi un modificēto T4 polimerāzi.
- 25. Paņēmiens saskaņā ar 24.punktu, kas turklāt ietver vismaz vienu papildu proteīnu, kurš veido kompleksu ar T4 polimerāzi, vai modificēto T4 polimerāzi, tādējādi intensificējot minētās T4 polimerāzes vai modificētās T4 polimerāzes procesēšanu.
- 26. Paņēmiens saskaņā ar 25. punktu, kur papildu proteīns ir izvēlēts no grupas, kas ietver T4 gēna produktus 32, 41, 45 un 44/62 kompleksu.
- 27. Paņēmiens saskaņā ar 23. punktu, kur modificētā polimerāze ir E.coli DNS polimerāze un modificētā E.coli DNS polimerāze ii.
- 28. Paņēmiens saskaņā ar 27.punktu, kas turklāt ietver vismaz vienu papildu proteīnu, kurš veido kompleksu ar E.coli DNS polimerāzi II vai modificēto E.coli DNS polimerāzi II, tādējādi intensificējot minētās E.coli DNS polimerāzes II un modificētās E.coli DNS polimerāzes II procesēšanu.
- 29. Paņēmiens saskaņā ar 28.punktu, kur minētais papildu proteīns ir β proteīna, γ kompleksa un vienpavediena nukleīnskābes piesaistoša proteīna kombinācija.
- 30. DNS sekvenēšanas paņēmiens, kas ietver: modificētās T4 DNS polimerāzes, kurai nav 3'—*5' eksonukleāzes aktivitātes, kontaktēšanu ar sekvenējamo DNS praimera pavedienu standarta nukleotīdu klātienē, pie kam vienam no standarta nukleotīdiem koncentrācija ir ļoti zema, salīdzinot ar citu standarta nukleotīdu koncentrāciju; un kontakta nodrošināšana reakcijas apstākļos, lai saglabātu polimerāzes aktivitāti pietiekami ilgu laiku sekvenēšanas informācijas iegūšanai. - 5 - LV 11486
- 31. Paņēmiens modificēto T4 DNS polimerāžu identificēšanai un izolēšanai, kurā: identificē T4 celmus ar modificētām T4 DNS polimerāzēm, kurām ir nepietiekama, pēc dažiem kritērijiem, DNS replikācija; izolē minēto modificēto T4 DNS polimerāžu tālāk modificētās formas, selekcionējot E.coli optA1 saimniekorganismā; izolē T4 celmus, kas satur modificētās T4 DNS polimerāzes ar vismaz vienu papildu mutāciju, kura koriģē vai kompensē minēto trūkumu DNS replikācijā; identificē papildu koriģējošo/kompensējošo mutāciju minētās modificētās T4 DNS polimerāzes gēnā un ievada identificēto koriģējošo/kompensējošo mutāciju T4 fāga vai T4 DNS polimerāzes ekspresijas vektorā.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/101,593 US5547859A (en) | 1993-08-02 | 1993-08-02 | Chain-terminating nucleotides for DNA sequencing methods |
| PCT/US1994/008610 WO1995004162A2 (en) | 1993-08-02 | 1994-08-01 | Dna sequencing enzymes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| LV11486A LV11486A (lv) | 1996-08-20 |
| LV11486B true LV11486B (en) | 1997-02-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LVP-96-23A LV11486B (en) | 1993-08-02 | 1996-02-14 | Dna sequencing enzymes |
Country Status (18)
| Country | Link |
|---|---|
| US (3) | US5547859A (lv) |
| EP (1) | EP0713535A1 (lv) |
| JP (1) | JPH09509301A (lv) |
| KR (1) | KR960704067A (lv) |
| CN (1) | CN1132529A (lv) |
| AU (1) | AU699498B2 (lv) |
| BG (1) | BG100188A (lv) |
| BR (1) | BR9407403A (lv) |
| CA (1) | CA2168471A1 (lv) |
| CZ (1) | CZ30696A3 (lv) |
| FI (1) | FI960469A (lv) |
| HU (1) | HUT75841A (lv) |
| LV (1) | LV11486B (lv) |
| NO (1) | NO960408L (lv) |
| NZ (1) | NZ269727A (lv) |
| PL (1) | PL312836A1 (lv) |
| SK (1) | SK14196A3 (lv) |
| WO (1) | WO1995004162A2 (lv) |
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| AU2804597A (en) * | 1996-04-15 | 1997-11-07 | University Of Alberta | Synthesis of fluorophore-labeled dna |
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| US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US6780591B2 (en) | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
| US20040009486A1 (en) | 1999-10-29 | 2004-01-15 | Sorge Joseph A. | Compositions and methods utilizing DNA polymerases |
| EP1226255B1 (en) * | 1999-10-29 | 2006-03-29 | Stratagene California | Compositions and methods utilizing dna polymerases |
| US6664079B2 (en) | 2000-10-06 | 2003-12-16 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
| US9708358B2 (en) | 2000-10-06 | 2017-07-18 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
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| DK3363809T3 (da) | 2002-08-23 | 2020-05-04 | Illumina Cambridge Ltd | Modificerede nukleotider til polynukleotidsekvensering |
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| WO2008069973A2 (en) | 2006-12-01 | 2008-06-12 | The Trustees Of Columbia University In The City Of New York | Four-color dna sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators |
| DK2725107T3 (da) | 2007-10-19 | 2019-01-02 | Univ Columbia | DNA-sekventering med ikke-fluorescerende nukleotidreversible terminatorer og ddNTP'er modificeret med spaltbart mærke og nukleinsyre omfattende inosin med reversible terminatorer |
| EP4310194A3 (en) | 2007-10-19 | 2024-10-16 | The Trustees of Columbia University in the City of New York | Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis |
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-
1993
- 1993-08-02 US US08/101,593 patent/US5547859A/en not_active Expired - Fee Related
-
1994
- 1994-08-01 WO PCT/US1994/008610 patent/WO1995004162A2/en not_active Application Discontinuation
- 1994-08-01 AU AU74088/94A patent/AU699498B2/en not_active Ceased
- 1994-08-01 NZ NZ269727A patent/NZ269727A/en unknown
- 1994-08-01 HU HU9600235A patent/HUT75841A/hu unknown
- 1994-08-01 PL PL94312836A patent/PL312836A1/xx unknown
- 1994-08-01 CZ CZ96306A patent/CZ30696A3/cs unknown
- 1994-08-01 JP JP7506010A patent/JPH09509301A/ja active Pending
- 1994-08-01 BR BR9407403A patent/BR9407403A/pt not_active Application Discontinuation
- 1994-08-01 CA CA002168471A patent/CA2168471A1/en not_active Abandoned
- 1994-08-01 EP EP94924079A patent/EP0713535A1/en not_active Withdrawn
- 1994-08-01 KR KR1019960700551A patent/KR960704067A/ko not_active Application Discontinuation
- 1994-08-01 SK SK141-96A patent/SK14196A3/sk unknown
- 1994-08-01 CN CN94193642A patent/CN1132529A/zh active Pending
-
1995
- 1995-06-06 US US08/465,994 patent/US5928919A/en not_active Expired - Fee Related
- 1995-06-06 US US08/465,995 patent/US5660980A/en not_active Expired - Fee Related
- 1995-12-04 BG BG100188A patent/BG100188A/xx unknown
-
1996
- 1996-01-31 NO NO960408A patent/NO960408L/no unknown
- 1996-02-01 FI FI960469A patent/FI960469A/fi not_active Application Discontinuation
- 1996-02-14 LV LVP-96-23A patent/LV11486B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US5660980A (en) | 1997-08-26 |
| CN1132529A (zh) | 1996-10-02 |
| NO960408L (no) | 1996-03-27 |
| LV11486A (lv) | 1996-08-20 |
| SK14196A3 (en) | 1996-06-05 |
| US5547859A (en) | 1996-08-20 |
| CZ30696A3 (en) | 1996-06-12 |
| HU9600235D0 (en) | 1996-03-28 |
| JPH09509301A (ja) | 1997-09-22 |
| PL312836A1 (en) | 1996-05-13 |
| AU699498B2 (en) | 1998-12-03 |
| NO960408D0 (no) | 1996-01-31 |
| BR9407403A (pt) | 1996-11-05 |
| AU7408894A (en) | 1995-02-28 |
| FI960469A0 (fi) | 1996-02-01 |
| CA2168471A1 (en) | 1995-02-09 |
| HUT75841A (en) | 1997-05-28 |
| KR960704067A (ko) | 1996-08-31 |
| NZ269727A (en) | 1997-11-24 |
| BG100188A (en) | 1996-12-31 |
| FI960469A (fi) | 1996-03-27 |
| WO1995004162A2 (en) | 1995-02-09 |
| WO1995004162A3 (en) | 1995-06-01 |
| US5928919A (en) | 1999-07-27 |
| EP0713535A1 (en) | 1996-05-29 |
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