LU502956B1 - A Piglet Feed Additive and Its Preparation Method - Google Patents

Abstract

The invention discloses a piglet feed additive, and it comprises 2-3 parts of yeast culture; 1-3 parts of organic acidifier; 0.1-0.2 part of plant extract; 0.5-1 part of probiotics; 2-4 parts of sodium humate; 0.3-0.5 part of antibacterial peptide; and 5-8 parts of carriers. The additive prepared by the invention can make weaned piglets maintain the normal digestive and absorption function of gastrointestinal tract without resistance or zinc oxide, resist or reduce the negative impact of various stress factors on the growth of piglets in the raising and production process, improve their health conditions, help piglets give full play to their production performance, and effectively alleviate and improve the negative impact of banning antibiotics and zinc oxide restriction on the organism. At the same time, the additive is slowly released in the digestive tract, and can adsorb harmful substances such as mycotoxins, and has certain anti-inflammatory and antioxidant functions.

Description

DESCRIPTION LUU502956

A Piglet Feed Additive and Its Preparation Method

TECHNICAL FIELD

The invention discloses a piglet feed additive and its preparation method thereof, in particular to a feed additive which can improve the utilization of nutrients and replace zinc oxide, and belongs to the technical field of animal nutrition and feed science.

BACKGROUND

Under the modern intensive breeding mode, in order to improve the reproductive performance of sows and save the feeding cost of sows, early weaning technology is usually adopted for piglets. However, early weaning will cause severe weaning stress of piglets. Weaning stress and the imperfect intestinal digestion and barrier function of piglets make them prone to weaning syndrome, with clinical manifestations of slow growth and diarrhea, which seriously affects the economic benefits of pig raisers. Only 50% of piglets ate food within 24h after early weaning, while 10% of piglets still didn't eat after 48h, which caused a large amount of energy overdraft of piglets, and the energy loss caused by weaning stress took about 8 ~ 14 days to recover. After weaning, the secretion of gastric acid in piglets decreased, the activities of trypsin and disaccharidase in digestive tract decreased, villi atrophied and crypts proliferated, which seriously affected the digestion and absorption of nutrition. The cessation of maternal antibody supply significantly inhibited the immune ability of piglets whose own immune system was not perfect, and the antibody level in blood decreased. Therefore, how to overcome the negative effects of weaning stress, maintain the intestinal health of piglets and improve the production performance is a common concern of nutritionists.

Zinc is another efficient trace element growth-promoting additive found in weaned piglets after copper. It can effectively relieve weaning stress of piglets, and has important physiological and nutritional significance for animal growth, reproduction, immunity, cell colonization and antioxidation. High-concentration zinc oxide has been proved to regulate intestinal bacteria flora and reduce histamine release in many studies artH}502356 practical production, and effectively improve diarrhea and growth retardation caused by weaning stress in piglets. Therefore, high-dose zinc oxide is widely used in the diets of weaned piglets. At present, the added amount of zinc in the compound feed for weaned piglets has reached 1600mg/kg, which is more than 10 times of the normal requirement.

However, zinc is a toxic heavy metal to most organisms, including pigs, and excessive concentration of zinc ions will cause great cytotoxicity. High dosage of zinc oxide has great potential safety hazards to animals, ecological environment and livestock and poultry products. Previous studies have found that daily intake of more than 50mg zinc for more than 12 weeks can lead to copper deficiency, which indicates that if high zinc is used in feed, it is necessary to add high copper to match it, which will easily lead to excessive copper and zinc content in animal liver. Adding 2500mg/kg zinc oxide had toxic effect on piglet liver, and vacuoles appeared in liver cytoplasm and fat deposition increased. Long-term use of high-dose zinc oxide will cause problems such as coarse fur, dull skin and later retarded growth and development of piglets. The vitro studies have found that high doses of zinc oxide may cause toxicity to CACO-2 cells and increase the permeability. In addition, most zinc ions can't be absorbed and directly discharged from the body after animals ingest high-dose zinc oxide, while zinc is a non-degradable substance. If it accumulates in water and soil for a long time, it will seriously destroy the ecological balance, which is not only potentially harmful to crops, but also a potential threat to human health.

It can be seen that the prohibition of high-dose zinc oxide will be the trend of future development, so it is one of the important problems to be solved urgently in pig industry to find a substitute for high-dose zinc oxide and solve piglet diarrhea.

SUMMARY

In view of this, the purpose of the present invention is to provide a zinc oxide substitute for preventing diarrhea of weaned piglets, its preparation method and application to overcome the defects of the prior technology.

Compared with high-dose zinc oxide, the zinc oxide substitute of the invention can improve the average daily gain (ADG) and feed/gain ratio (F/G) of piglets, increase tHa/202956 apparent digestibility of dry matter, crude protein, crude ash, crude fat and zinc, increase the height of villi and the ratio of villi to crypts, and reduce the depth of crypts, up-regulate the relative expression of IL-10, ZO-1, Occludin and MUC2 mRNA in jejunum, down-regulate the relative expression of mRNA, increase the number of beneficial bacteria Lactobacillus and Bifidobacterium in cecum, and reduce the number of harmful bacteria Escherichia coli in cecum.

In order to achieve the above object, the present invention provides the following technical schemes: a piglet feed additive comprises 2-3 parts of yeast culture; 1-3 parts of organic acidifier; 0.1-0.2 part of plant extract; 0.5-1 part of probiotics; 2-4 parts of sodium humate; 0.3-0.5 part of antibacterial peptide; and 5-8 parts of carriers.

Further, the piglet feed additive comprises 3 parts of yeast culture; 2 parts of organic acidifier; 0.1 part of plant extract; 1 part of probiotics; 3 parts of sodium humate; 0.5 part of antibacterial peptide; and 6 parts of carriers.

Further, the organic acidifier is prepared from the following raw materials in mass ratio: the mass ratio of the hawthorn powder, benzoic acid, fumaric acid and citric acid is 2-3:5:1-1.5:1-1.5.

Further, the organic acidifier is prepared from the following raw materials in mass ratio: the mass ratio of the hawthorn powder, benzoic acid, fumaric acid and citric acid is 2:5:1:1.

Further, the organic acidifier is prepared by the following method: (1) putting hawthorn fruit into an extracting tank, boiling it for 2 hours with 10 times of water and the steam pressure of 1Mpa, filtering and separating to obtain the primary filtrate and filter residue; (2) boiling the primary filter residue again for 2 hours with 10 times of water and the steam pressure of 1Mpa, filtering and separating to obtain the secondary filtrate and filter residue; (3) boiling the secondary filter residue again for 1 hour with 5 times of water and the steam pressure is 1Mpa, filtering and separating to obtain the tertiary filtrate and filter residue, and discarding the filter residue;

(4) mixing the primary filtrate, the secondary filtrate and the tertiary filtrate, artH}502356 concentrating them to 1/4 of the original volume; (5) pouring the concentrated mixed filtrate into spray drier to obtain the hawthorn powder for later use; (6) pouring benzoic acid, fumaric acid and citric acid into a stirrer and stirring for 3 minutes at a stirring speed of 120 rpm to prepare the first mixed acidifier; (7) pouring hawthorn powder into a mixer, and mixing it with the first Mixed acidifier for 8 minutes, with the stirring speed of 180 rpm, to make the finished product of organic acidifier.

Further, the plant extract is prepared by the following raw materials in mass ratio: the mass ratio of cinnamaldehyde, carvacrol, thymol, capsaicin and curcumin is 2-3: 4-5: 1-2: 0.5-1: 0.51.

Further, the plant extract is prepared by the following raw materials in mass ratio: the mass ratio of cinnamaldehyde, carvacrol, thymol, capsaicin and curcumin is 2:4:1:0.5:1.

Further, the plant extract is prepared by the following method: (1) adding cinnamaldehyde, carvacrol and thymol into a stirrer, and stirring for 3 minutes at a stirring speed of 120 rpm to prepare the first mixed plant extract; (2) pouring capsaicin and curcumin into a stirrer, and stirring for 3 minutes at a stirring speed of 120 rpm to prepare the second mixed plant extract; (3) pouring the first mixed plant extract and the second mixed plant extract into a stirrer, and stirring for 8 minutes at a stirring speed of 180 rpm to prepare the finished plant extract.

Further, the probiotics are prepared by the following raw materials in mass ratio: the mass ratio of Bacillus subtilis to Bacillus licheniformis is 1-2:2-3.

Further, the mass ratio of Bacillus subtilis to Bacillus licheniformis is 2:3.

Further, the sodium humate is coated sodium humate; and the carrier is unite bran.

The invention also provides a method for preparing the piglet feed additive and it comprises the following steps: (1) dividing the carriers into three parts and sodium humate into three parts;

(2) mixing organic acidifier and antibacterial peptide to obtain the first mixture; LU502956 (3) mixing the first mixture with the first part sodium humate to obtain the second mixture: (4) mixing the second mixture with the first part carrier to obtain the third mixture; (5) mixing the plant extract with probiotics to obtain the fourth mixture; (6) mixing the fourth mixture with the second part sodium humate to obtain the fifth mixture; (7) mixing the fifth mixture with the second carrier to obtain the sixth mixture; (8) mixing the yeast culture with the third part of sodium humate to obtain the seventh mixture; (9) mixing the third mixture, the sixth mixture and the seventh mixture with the third part carrier to obtain the piglet feed additive.

Further, the mass ratio of the first part carrier, the second part carrier and the third part carrier is 3-5:1-3:4-7, and the mass ratio of the first part sodium humate, second part sodium humate and third part sodium humate is 3-6:1-3:4-6.

Further, the mass ratio of the first part carrier, the second part carrier and the third part carrier is 4:2:6, and the mass ratio of the first part sodium humate, second part sodium humate and third part sodium humate is 5:2:5.

The invention also provides the piglet feed additive prepared according to any one of the preparation methods.

The invention also provides the application of any of the piglet feed additives, wherein the feed additive is added into the weaned piglet diet, and the added amount of the feed additive is 0.3-0.5% of the basic diet.

The raw materials used in the invention are all commercially available conventional food-grade products.

The invention has the beneficial effects that:

The piglets feed additive prepared by that invention effectively improve the defect of animal body and ecological environment imbalance caused by the traditional piglets feeding which must rely on zinc oxide. Yeast culture, organic acidifier, plant extract, probiotics, sodium humate, antibacterial peptide and carriers are used as the formula of the invention, which can not only improve the intestinal barrier function of piglets/°02956 maintain intestinal microecological balance, improve nutrient digestibility, but also promote the healthy growth of piglets.

Specific performance in the following aspects:

(1) After weaning, using the feed additive without resistance or zinc oxide can maintain the normal digestion and absorption function of gastrointestinal tract, resist or reduce the negative effects of various stress factors on the growth of piglets during the raising and production process, improve their health condition, help piglets maximize their production performance, and effectively alleviate and improve the negative effects of banning antibiotics and zinc oxide restrictions on the organism.

(2) The feed additive can adjust the pH value of piglet digestive tract, activate digestive enzymes, regulate intestinal bacteria flora, reduce diarrhea rate, increase piglets’ appetite and improve piglet's digestive ability to the feed; In addition, the feed additive can not only reduce the binding ability of the pH value and acid of the feed, inhibit the growth and reproduction of pathogenic microorganisms in the feed, and prevent the feed from mildewing, but also inhibit the proliferation of harmful bacteria.

(3) The feed additive can destroy the cell wall, cell membrane and membrane protein structure, increase its permeability, and lead to the overflow of cell contents, thus playing an antibacterial role.

Besides, it has antioxidant function, and it can accelerate intestinal peristalsis, increase the secretion of substances such as saliva, bile and related enzymes that contribute to digestion, so as to improve the digestion and utilization rate of nutrients.

And at the same time, it can significantly inhibit the expression amount of TNF-a to improve the anti-inflammatory potential of the organism.

(4) The feed additive can produce rich beneficial active metabolites in animals, degrade the anti-nutritional ingredients in the feed to a certain extent, reduce the obstacles to animals’ digestion, absorption and utilization of effective nutrients, so as to jointly maintain intestinal health.

(5) The feed additive will not react with acidifier in feed, and will not change the appearance color of feed; and it can slowly release in the digestive tract, it can adjust the intestinal electrolyte balance and adsorb harmful substances such as mycotoxins; and it can form a film on the surface of intestinal tract to reduce the damage of harmful substances to intestinal tract; what's more, it has certain astringent effect, water ari 202956 sodium retention, and also has certain anti-inflammatory and antioxidant functions. (6) The feed additive has a harmonious interaction with various factors of animal intestinal microenvironment (microecology), and is not affected by digestive enzymes. It can not only kill Gram's positive and negative pathogenic bacteria, but also have no inhibitory effect on Lactic acid bacteria and Bacillus. And it can increase the ratio of

Lactic acid bacteria to Escherichia coli in intestinal contents, improve intestinal barrier structure, reduce serum endotoxin content, improve piglet survival rate and feed conversion rate, and promote healthy growth of piglets.

It needs to be explained that the technical effect of the invention is the result of the mutual cooperation and interaction of process steps and parameters, not the superposition of simple processes. The effect produced by the organic combination of each process far exceeds the superposition of the functions and effects of each single process, and it has better advancement and practicability.

DETAILED DESCRIPTION OF THE INVENTION

The invention will be described in detail with reference to the following specific embodiments, but the scope of the invention will not be limited. The mass ratio in each embodiment is the mass parts ratio.

Embodiment

The preparation method of the piglet feed additive comprises the following steps: 1. Preparing the organic acidifier: (1) putting hawthorn fruit into an extracting tank, boiling it for 2 hours with 10 times of water and the steam pressure of 1Mpa, filtering and separating to obtain the primary filtrate and filter residue; (2) boiling the primary filter residue again for 2 hours with 10 times of water and the steam pressure of 1Mpa, filtering and separating to obtain the secondary filtrate and filter residue; (3) boiling the secondary filter residue again for 1 hour with 5 times of water and the steam pressure is 1Mpa, filtering and separating to obtain the tertiary filtrate and filter residue, and discarding the filter residue; LU502856 (4) mixing the primary filtrate, the secondary filtrate and the tertiary filtrate, and concentrating them to 1/4 of the original volume; (5) pouring the concentrated mixed filtrate into spray drier to obtain the hawthorn powder for later use; (6) mixing benzoic acid, fumaric acid and citric acid according to the mass ratio of 5:1-1.5:1-1.5, and then pouring them into a stirrer and stirring for 3 minutes at a stirring speed of 120 rpm to prepare the first mixed acidifier; (7) pouring 2-3 parts of hawthorn powder into a mixer, and mixing it with the first mixed acidifier for 8 minutes, with the stirring speed of 180 rpm, to make the finished product of organic acidifier. 2. Preparing the plant extract: (1) adding cinnamaldehyde, carvacrol and thymol into a stirrer according to the mass ratio of 2-3:4-5:1-2, and stirring for 3 minutes at a stirring speed of 120 rpm to prepare the first mixed plant extract; (2) pouring 0.5-1 part of capsaicin and 0.5-1 part of curcumin into a stirrer, and stirring for 3 minutes at a stirring speed of 120 rpm to prepare the second mixed plant extract; (3) pouring the first mixed plant extract and the second mixed plant extract into a stirrer, and stirring for 8 minutes at a stirring speed of 180 rpm to prepare the finished plant extract. 3. Preparing the probiotics: mixing Bacillus subtilis and Bacillus licheniformis accroding to the mass ratio of 1-2:2-3 to make the finished probiotic product. 4. The sodium humate is coated sodium humate, and the carrier is unite bran. 5. Preparing the piglet feed additive:

Taking 2-3 parts of yeast culture; 1-3 parts of organic acidifier; 0.1-0.2 part of plant extract; 0.5-1 part of probiotics; 2-4 parts of sodium humate(coated sodium humate); 0.3-0.5 part of antibacterial peptide; and 5-8 parts of carriers(unite bran). (1) dividing the carriers into three parts and the mass ratio of the first part carrier, the second part carrier and the third part carrier is 3-5:1-3:4-7; and dividing the sodium humate into three parts and the mass ratio of the first part sodium humate, second part/°02956 sodium humate and third part sodium humate is 3-6:1-3:4-6; (2) mixing organic acidifier and antibacterial peptide to obtain the first mixture; (3) mixing the first mixture with the first part sodium humate to obtain the second mixture: (4) mixing the second mixture with the first part carrier to obtain the third mixture; (5) mixing the plant extract with probiotics to obtain the fourth mixture; (6) mixing the fourth mixture with the second part sodium humate to obtain the fifth mixture; (7) mixing the fifth mixture with the second carrier to obtain the sixth mixture; (8) mixing the yeast culture with the third part of sodium humate to obtain the seventh mixture; (9) mixing the third mixture, the sixth mixture and the seventh mixture with the third part carrier to obtain the piglet feed additive and adding the feed additive into the weaned piglet diet, and the added amount of the feed additive is 0.3-0.5% of the basic diet.

Embodiment 1

The piglet feed additive

The additive comprises the following raw materials in parts by mass: 2 parts of yeast culture; 2 parts of organic acidifier; 0.2 part of plant extract; 1 part of probiotics; 2 parts of sodium humate; 0.4 part of antibacterial peptide; and 6 parts of carriers.

The preparation method of the piglet feed additive comprises the following steps: 1. Preparing the organic acidifier: (1) putting hawthorn fruit into an extracting tank, boiling it for 2 hours with 10 times of water and the steam pressure of 1Mpa, filtering and separating to obtain the primary filtrate and filter residue; (2) boiling the primary filter residue again for 2 hours with 10 times of water and the steam pressure of 1Mpa, filtering and separating to obtain the secondary filtrate and filter residue; (3) boiling the secondary filter residue again for 1 hour with 5 times of water and the steam pressure is 1Mpa, filtering and separating to obtain the tertiary filtrate and filtkH 502956 residue, and discarding the filter residue;

(4) mixing the primary filtrate, the secondary filtrate and the tertiary filtrate, and concentrating them to 1/4 of the original volume;

(5) pouring the concentrated mixed filtrate into spray drier to obtain the hawthorn powder for later use;

(6) mixing benzoic acid, fumaric acid and citric acid according to the mass ratio of 5:1:1, and then pouring them into a stirrer and stirring for 3 minutes at a stirring speed of 120 rpm to prepare the first mixed acidifier;

(7) pouring 2 parts of hawthorn powder into a mixer, and mixing it with the first mixed acidifier for 8 minutes, with the stirring speed of 180 rpm, to make the finished product of organic acidifier.

2. Preparing the plant extract:

(1) adding cinnamaldehyde, carvacrol and thymol into a stirrer according to the mass ratio of 2:4:1, and stirring for 3 minutes at a stirring speed of 120 rpm to prepare the first mixed plant extract;

(2) pouring 0.5 part of capsaicin and 0.5 part of curcumin into a stirrer, and stirring for 3 minutes at a stirring speed of 120 rpm to prepare the second mixed plant extract;

(3) pouring the first mixed plant extract and the second mixed plant extract into a stirrer, and stirring for 8 minutes at a stirring speed of 180 rpm to prepare the finished plant extract.

3. Preparing the probiotics: mixing Bacillus subtilis and Bacillus licheniformis accroding to the mass ratio of 1:2 to make the finished probiotic product.

4. The sodium humate is coated sodium humate; and the carrier is unite bran.

5. Preparing the piglet feed additive:

(1) dividing the carriers into three parts and the mass ratio of the first part carrier, the second part carrier and the third part carrier is 3:1:4; and dividing the sodium humate into three parts and the mass ratio of the first part sodium humate, second part sodium humate and third part sodium humate is 3:1:4;

(2) mixing organic acidifier and antibacterial peptide to obtain the first mixture;

(3) mixing the first mixture with the first part sodium humate to obtain the secork 502956 mixture; (4) mixing the second mixture with the first part carrier to obtain the third mixture; (5) mixing the plant extract with probiotics to obtain the fourth mixture; (6) mixing the fourth mixture with the second part sodium humate to obtain the fifth mixture; (7) mixing the fifth mixture with the second carrier to obtain the sixth mixture; (8) mixing the yeast culture with the third part of sodium humate to obtain the seventh mixture; (9) mixing the third mixture, the sixth mixture and the seventh mixture with the third part carrier to obtain the piglet feed additive and adding the feed additive into the weaned piglet diet, and the added amount of the feed additive is 0.3-0.5% of the basic diet.

Embodiment 2

The piglet feed additive

The additive comprises the following raw materials in parts by mass: 3 parts of yeast culture; 2 parts of organic acidifier; 0.1 part of plant extract; 1 part of probiotics; 3 parts of sodium humate; 0.5 part of antibacterial peptide; and 6 parts of carriers.

The preparation method of the piglet feed additive is as follows: 1. Preparing organic acidifier: (1) putting hawthorn fruit into an extracting tank, boiling it for 2 hours with 10 times of water and the steam pressure of 1Mpa, filtering and separating to obtain the primary filtrate and filter residue; (2) boiling the primary filter residue again for 2 hours with 10 times of water and the steam pressure of 1Mpa, filtering and separating to obtain the secondary filtrate and filter residue; (3) boiling the secondary filter residue again for 1 hour with 5 times of water and the steam pressure is 1Mpa, filtering and separating to obtain the tertiary filtrate and filter residue, and discarding the filter residue; (4) mixing the primary filtrate, the secondary filtrate and the tertiary filtrate, and concentrating them to 1/4 of the original volume; LU502956

(5) pouring the concentrated mixed filtrate into spray drier to obtain the hawthorn powder for later use;

(6) mixing benzoic acid, fumaric acid and citric acid according to the mass ratio of 5:1.5:1.5, and then pouring them into a stirrer and stirring for 3 minutes at a stirring speed of 120 rpm to prepare the first mixed acidifier;

(7) pouring 3 parts of hawthorn powder into a mixer, and mixing it with the first mixed acidifier for 8 minutes, with the stirring speed of 180 rpm, to make the finished product of organic acidifier.

2. Preparing the plant extract:

(1) adding cinnamaldehyde, carvacrol and thymol into a stirrer according to the mass ratio of 3:5:2, and stirring for 3 minutes at a stirring speed of 120 rpm to prepare the first mixed plant extract;

(2) pouring 1 part of capsaicin and 1 part of curcumin into a stirrer, and stirring for 3 minutes at a stirring speed of 120 rpm to prepare the second mixed plant extract;

(3) pouring the first mixed plant extract and the second mixed plant extract into a stirrer, and stirring for 8 minutes at a stirring speed of 180 rpm to prepare the finished plant extract.

3. Preparing the probiotics: mixing Bacillus subtilis and Bacillus licheniformis accroding to the mass ratio of 2:3 to make the finished probiotic product.

4. The sodium humate is coated sodium humate; and the carrier is unite bran.

5. Preparing the piglet feed additive:

(1) dividing the carriers into three parts and the mass ratio of the first part carrier, the second part carrier and the third part carrier is 5:3:7; and dividing the sodium humate into three parts and the mass ratio of the first part sodium humate, second part sodium humate and third part sodium humate is 6:3:6;

(2) mixing organic acidifier and antibacterial peptide to obtain the first mixture;

(3) mixing the first mixture with the first part sodium humate to obtain the second mixture;

(4) mixing the second mixture with the first part carrier to obtain the third mixture;

(5) mixing the plant extract with probiotics to obtain the fourth mixture; LU502956 (6) mixing the fourth mixture with the second part sodium humate to obtain the fifth mixture: (7) mixing the fifth mixture with the second carrier to obtain the sixth mixture; (8) mixing the yeast culture with the third part of sodium humate to obtain the seventh mixture: (9) mixing the third mixture, the sixth mixture and the seventh mixture with the third part carrier to obtain the piglet feed additive and adding the feed additive into the weaned piglet diet, and the added amount of the feed additive is 0.3-0.5% of the basic diet.

In order to verify that the formulas of the piglet feed additive prepared by the invention do have remarkable synergistic effect, the following comparative examples are set to further verify its technical effect.

Comparative example 1

The piglet feed additive

Compared with embodiment 1, the additive cancels the use of yeast culture, and the rest of the process and formula are the same as embodiment 1, including: 2 parts of organic acid, 0.2 parts of plant extract, 1 part of probiotics, 2 parts of sodium humate, 0.4 parts of antibacterial peptide and 6 parts of carriers.

Comparative example 2

The piglet feed additive

Compared with embodiment 1, the additive cancels the use of yeast culture, and the rest of the process and formula are the same as embodiment 1, including: 2 parts of yeast culture, 0.2 part of plant extract, 1 part of probiotics, 2 parts of sodium humate, 0.4 part of antibacterial peptide and 6 parts of carriers.

Comparative example 3

The piglet feed additive

Compared with embodiment 1, the additive cancels the use of yeast culture, and the rest of the process and formula are the same as embodiment 1, including: 2 parts of yeast culture, 2 parts of organic acid, 1 part of probiotics, 2 parts of sodium humate, 0.4 parts of antibacterial peptide and 6 parts of carriers. LU502956

Comparative example 4

The piglet feed additive

Compared with embodiment 1, the additive cancels the use of yeast culture, and the rest of the process and formula are the same as embodiment 1, including: 2 parts of yeast culture, 2 parts of organic acid, 0.2 parts of plant extract, 2 parts of sodium humate, 0.4 parts of antibacterial peptide and 6 parts of carrier.

Comparative example 5

The piglet feed additive

Compared with embodiment 1, the additive cancels the use of yeast culture, and the rest of the process and formula are the same as embodiment 1, including: 2 parts of yeast culture, 2 parts of organic acid, 0.2 part of plant extract, 1 part of probiotics, 0.4 part of antibacterial peptide and 6 parts of carriers.

Comparative example 6

The piglet feed additive

Compared with embodiment 1, the additive cancels the use of yeast culture, and the rest of the process and formula are the same as embodiment 1, including: 2 parts of yeast culture, 2 parts of organic acid, 0.2 part of plant extract, 1 part of probiotics, 2 parts of sodium humate and 6 parts of carriers.

Test example 1

In this experiment, 216 DLY piglets weaned at 28 days of age and weighing 9 kg on average are divided into 9 treatments, with 6 replicates in each treatment and 4 pigs in each replicate. The trial period lasted for 14 days.

The diets of piglets in each treatment group are as follows:

Treatment 1 is the basic diet containing zinc oxide (see Table 1 for the basic diet formula, and the zinc oxide content is 1500mg/kg), which is used as the observation control group.

Treatment 2 is the basic diet supplemented with 0.4% of the additive described in embodiment 1, which is used as the embodiment 1 group.

Treatment 3 is the basic diet supplemented with 0.4% of the additive described in embodiment 2, which is used as the embodiment 2 group. LU502956

Treatment 4 is the basic diet supplemented with 0.4% of the additive described in comparative example 1, which is used as the comparative example 1 group.

Treatment 5 is the basic diet supplemented with 0.4% of the additive described in comparative example 2, which is used as the comparative example 2 group.

Treatment 6 is the basic diet supplemented with 0.4% of the additive described in comparative example 3, which is used as the comparative example 3 group.

Treatment 7 is the basic diet supplemented with 0.4% of the additive described in comparative example 4, which is used as the comparative example 4 group.

Treatment 8 is the basic diet supplemented with 0.4% of the additive described in comparative example 5, which is used as the comparative example 5 group.

And treatment 9 is the basic diet supplemented with 0.4% of the additive described in comparative example 6, which is used as the comparative example 6 group.

Table 1 Formula and nutritional components of pig feed basic diet LU502956 7 Rewmaterels_ rome oran

Calcium 1.81 ocre || coms | um [remous | om Aves phosphos | 060 [yes | os renee ow [om ow 1

Trace element premix

Te ew

Nennpemess | ow ww

At the beginning and end of the experiment, weighing each piglet and the diet in each replicate, and calculating the average daily gain (ADG), average daily feed intake (ADFI) and feed/gain ratio (F/G); During the whole experiment, observing the feces of each pig and calculating the diarrhea rate of piglets in different groups. From the 11th to 14th day of the experiment, using the feces collection method with endogenous indicator to carry out the digestion trial and collecting rectal feces sample. And adding 10% sulfuric acid and toluene for nitrogen fixation and preservation, fully mixing the collected feces of pigs in each replicate, baking at 60~65°C to constant weight, drying, crushing, sieving with a 40-mesh sieve, and determining the contents of crude protein (CP), ether extract (EE), dry matter (DM), crude ash (CA) and zinc (Zn) in feces. At the end of the experiment, selecting one piglet for slaughter in each replicate. Taking intact duodenum, jejunum and ileum and preserving them in 4% paraformaldehyde solution, and measuring the intestinal morphology. And taking jejunum mucosa to measure the relative!©02956 expression of jejunal barrier-related genes, such as interleukin 10(IL-10), interleukin 1B(IL-1B), mucin 1(MUC1), mucin 2(MUC2), occludin and zonula occluden (ZO-1), and taking cecum chyme to measure the number of cecal microorganisms.

Using Excel 2010 for data statistics, and using SAS 8.0 statistical software for analysis of variance(ANOVA). And using the Duncan's method for multiple comparison of significant differences, with P<0.05 as the criterion for judging the significance of differences, P < 0.05 as the significant difference, and 0.05 <P < 0.10 as a trend. The test results are shown in Table 2 to Table 6.

Table 2 Effect of zinc oxide substitution on growth performance and diarrhea score of-V502956 weaned piglets

Obs P

Em | Em | Comp | Comp | Comp | Comp | Comp | Comp ervat bod | bodi | arativ | arativ | arativ | arativ | arativ | arativ ion S|v

Item ime | men e e e e e e contr E a

Ss nt1| t2 | exam | exam | exam | exam | exam | exam ol MI gro | gro | ple1 | ple2 | ple3 | ple4 | ple5 | ple6 grou u up | up | group | group | group | group | group | group p e

Initia 00 a 9.08 | 9.0 9.08 weig 9.088 | 9.091 | 9.085 | 9.084 | 9.087 | 9.084 11 9 8 91 3 ht 319 (kg) 318 14 d 010

Final |. 125 | 12. | 12.6 | 12.55 | 12.58 | 12.55 | 12.51 | 12.55 | 12.64 weig 119 05 [612] 96 5 4 4 6 4 0 ht 318 (kg) 315 510 403 |.

ADF | 415. 410. | 407.0 | 411.9 | 409.5 | 403.0 | 420.6 | 413.6 22 313

I (g) | 581 630 69 86 95 76 79 85 216 514 210 251 | 258. |.

ADG | 244. 247.6 | 2495 | 247.8 | 245.1 | 247.6 | 254.0

A7 | 271 410 (g) | 107° 92ab 038 812 94° 382 25% qab a 411 4 | 1 1.643 | 1.652 | 1.653 | 1.644 | 1.699 | 1.630 0/0

3 03 | LU502956 011 21 814 010

Diarr hea | 5.25 | 54 | 4.17 hE 5610 | 5.713 | 5.600 | 5.678 | 6.213 | 5.423 | 412 rate( 1 04 9 718 %) 811

Note: Different letters on the same shoulder in the table indicate significant differences (P < 0.05), and the following table is the same.

As shown in table 2, compared with the observation control group, the ADG of embodiment 2 group is significantly increased (P < 0.05). In terms of piglet diarrhea rate, there is no significant difference among the observation control group, the feed additive group of the invention and the comparative group, but from the numerical value of view, the diarrhea rate of weaned piglets can be reduced by 20.42% by using the embodiment 2 group. To sum up, it shows that the feed additive of the invention can completely replace zinc oxide to control piglet diarrhea.

Table 3 Effect of zinc oxide substitution on nutrient digestibility of weaned piglets LU502956

Comp | Comp | Comp

Obser Comp | Comp

Embo | Embo arativ | arativ | arativ vation arative | arative P dimen | dimen e e e SE

Items | contro examp | examp val t1 t2 exam | exam | exam | M le 1 le 2 ue group | group ple 3 | ple5 | ple6 group group | group group | group | group

Ether 0. 72.40 | 74.51 | 75.72 | 73.05 | 73.29 | 72.84 | 73.30 | 74.10 0.0 extract(E 63 2a 7ab 1 b 2ab 5ab gab ab gab 40

E) 6

Dry 0. 82.79 | 86.20 | 87.10 | 84.68 | 84.56 | 85.79 | 85.25 | 86.05 0.0 matter(D 57 gb Ga 2a gab gab Ga 4ab 7a 02

M) 0 0.

Crude | 58.82 | 65.79 | 65.44 | 61.33 | 62.58 | 65.32 | 64.68 | 65.14 <0. 76 ash(CA) 4° 28 8? gbe Oab 4? Q@b 28 001 2

Crude 0. 70.49 | 78.48 | 79.62 | 72.30 | 73.67 | 76.48 | 76.09 | 77.75 <0. protein( 63 4¢ 5ab Qa gde 4°d gbe 7bc gab 001

CP) 6 1. 31.82 | 40.89 | 37.90 | 35.60 | 36.31 | 37.05 | 36.95 | 37.22 0.0

Zinc(Zn) 09 8° 4ab 5a 5bc abc 1 abc abc 3ab 02 2

As shown in table 3, compared with the observation control group, the digestibility of ether extract of weaned piglets in embodiment 2 group can be significantly increased (P < 0.05); Compared with the observation control group, the digestibility of dry matter of weaned piglets in embodiment 1 group, embodiment 2 group, comparative example 3 group, comparative example 4 group and comparative example 6 group can be significantly increased (P < 0.05). Compared with the observation control group, the digestibility of crude ash and crude protein of weaned piglets can be significantly increased in embodiment 1 group, embodiment 2 group, comparative example 2 group,

comparative example 3 group, comparative example 4 group, comparative example 14/502956 group and comparative example 6 group (P < 0.05). Compared with the observation control group, the digestibility of the zinc of weaned piglets in embodiment 1 group, embodiment 2 group, and comparative example 6 group can be significantly improved (P < 0.05). And compared with embodiment 1 group and embodiment 2 group, the digestibility of crude ash of weaned piglets in comparative example 1 group decreases significantly (P < 0.05). Compared with the embodiment 1 group, the digestibility of crude protein of weaned piglets in comparative example 1 group and comparative example 2 group decreases significantly (P < 0.05). Compared with the embodiment 2 group, the digestibility of crude protein of weaned piglets in comparative example 1 group, comparative example 2 group, comparative example 3 group, comparative example 4 group and comparative example 5 group decreases significantly (P < 0.05). Compared with the embodiment 2 group, the digestibility of zinc of the weaned piglets in comparative example 1 group decreases significantly (P < 0.05). To sum up, it shows that the feed additive of the invention is superior to zinc oxide in improving nutrient digestibility of piglets.

Table 4 Effect of zinc oxide substitution on intestinal morphology of weaned piglets LU502956

Obs Com | Com | Com | Com | Com | Com ervat | Emb | Emb | parat | parat | parat | parat | parat | parat ion | odim | odim ive ive ive ive ive ive S| P

Items contr | ent 1 | ent2 | exam | exam | exam | exam | exam | exam | E | val ol grou | grou | ple 1 | ple2 | ple3 | pled | ple5 | ple6 | M | ue grou p p grou | grou | grou | grou | grou | grou p p p p p p p

Villus 10 | O. 367. | 389. | 377. | 373. | 372. | 378. | 387. | 373. | 369. height(u 5183 632 | 543 210 443 507 312 699 851 690 m) 39 | 4

Crypt 6. | 0. 149. | 115. | 109. | 129. | 122. | 118. | 125. | 118. | 117. depth(pm 13 | 00 2132 | 408° | 690P | 578% | 533% | 838P 985% | 503P | 748P ) 6 9

The ratio 0. | O. 246 | 338 | 344 | 290 | 3.07 | 3.21 | 3.11 | 3.20 | 3.19 of villi to 20 | 08 0 3 5 5 3 0 5 0 crypt 1 2

Villus 391. 8. | <0 332. | 410. | 417. | 361. | 369. | 400. 418. | 402. height(u 2302b 01 | .O 9029 | 1662 | 3282 | 180€ | 272¢d | 355% 4332 | 492% m) c 8 | 01

Crypt 6. | 0. 131. | 127. | 126. | 132. | 126. | 128. | 128. | 133. | 128. depth(pm 83 89 600 | 503 595 435 913 638 373 000 565 ) 4 | 8

The ratio 0. | O. 254 | 322 | 3.30 | 2.76 | 292 | 3.11 | 3.08 | 3.20 | 3.13 of villi to 15 | 04 5b Qab 5a gab gab 5ab 3ab gab 5ab crypt 7 | 5 vis [aos ] 324 [34 | 314 | or [arr 10 [20 [are [12] 0 height(u | 041 | 390 | 052 | 221 | 377 | 063 | 256 | 989 | 813 | 7 | 5p02956

REEL

Crypt 2. | 0. 137. | 127. | 121. | 132. | 129. | 131. | 129. | 132. | 128. depth(pm 34 | 00 219 | 255 | 285 | 238 | 237 | 241 | 240 | 241 | 2.47 of villi to 11 | 06 8 0 0 0 8 8 8 8 0 crypt 8 | 1

As shown in table 4, compared with the observation control group, the depth of duodenal crypt of weaned piglets can be significantly reduced by using the embodiment 1 group, embodiment 2 group, comparative example 3 group, comparative example 5 group and comparative example 6 group of the present invention(P < 0.05); Compared with the observation control group, the height of jejunum villi of weaned piglets can be significantly increased by using embodiment 1 group, embodiment 2 group, comparative example 3 group, comparative example 4 group, comparative example 5 group and comparative example 6 group of the present invention(P < 0.05); And compared with the observation control group, the ratio of villi to crypt of jejunum of weaned piglets is significantly increased and the depth of ileum crypt is significantly decreased by using the embodiment 2 group of the present invention(P < 0.05). Compared with the observation control group, the ratio of villi to crypt of duodenum (P = 0.082) and ileum (P = 0.061) of weaned piglets in embodiment 2 group of the present invention has a tendency to increase. Compared with embodiment 1 group and embodiment 2 group, the height of jejunum villi of weaned piglets in comparative example 1 group and comparative example 2 group decreases significantly (P < 0.05). There is no significant difference in the height of villi of jejunum, duodenum and ileum and the crypt depth of jejunum among the treatment groups (P > 0.05). To sum up, it shows that the feed additive of the invention is superior to zinc oxide in improving the intestinal morphology of piglets.

Table 5 Effect of zinc oxide substitution on relative expression amount of jejunum barrigH°02956 related gene mRNA in weaned piglets

Obse Comp | Comp | Comp | Comp | Comp | Comp rvatio | Embo | Embo | arativ | arativ | arativ | arativ | arativ | arativ S P

Ite n dimen | dimen e e e e e e va

E ms | contr t1 t2 exam | exam | exam | exam | exam | exam lu

M ol group | group | ple 1 | ple2 | ple3 | ple4 | ple5 | ple6 e group group | group | group | group | group | group 0. | 0.

IL-1 | 1.000 | 1.690 | 1.564 | 1.309 | 1.283 | 1.402 | 1.525 | 1.511 | 1.447 0 b a ab ab ab ab ab ab ab 12103 6 | 8 0. | 0.

IL-1 1.000 | 0.621 | 0.640 | 0.845 | 0.722 | 0.693 | 0.719 | 0.697 | 0.666 | 08 | 08

B

316 0. | 0.

MU

1.000 | 1.142 | 1.182 | 1.068 | 0.993 | 1.079 | 0.941 | 1.049 | 0.932 | 15 | 95

C1 2 | 3 0. | 0.

MU | 1.000 | 1.466 | 1.522 | 1.258 | 1.410 | 1.329 | 1.427 | 1.493 | 1.414 09 | 03

C2 b ab a ab ab ab ab a ab 9 2

Occ 0. | 0. 1.000 ludi 1.422 | 1.776 | 1.270 | 1.357 | 1.403 | 1.374 | 1.255 | 1.394 | 15 | 14 b n 4 | 4 0. | 0.

ZO- | 1.000 | 1.682 | 1.536 | 1.193 | 1.448 | 1.432 | 1.373 | 1.424 | 1.507 1 b a a b ab ab ab ab ab 11 02 9 8

As shown in table 5, compared with the observation control group, the relative expression amount of ZO-1 mRNA in jejunum of weaned piglets significantly increases by using the embodiment 1 group and embodiment 2 group of the present invention (P < 0.05); Compared with the observation control group, the relative expression amount of

IL-10 mRNA in jejunum of weaned piglets significantly increases by using He)002956 embodiment 1 group of the present invention (P < 0.05); And compared with the observation control group, the relative expression amount of MUC2 mRNA in jejunum of weaned piglets significantly increases by using the embodiment 2 group and the comparative example 5 group (P < 0.05). Compared with the embodiment 1 group and embodiment 2 group, the relative expression amount of ZO-1 mRNA in jejunum of piglets in comparative example 1 group decreases significantly (P < 0.05). Compared with the observation control group, the relative expression amount of jejunal IL-18 mRNA in the embodiment 1 group and embodiment 2 group of the invention tends to be decreased (P = 0.086). To sum up, it shows that the feed additive of the invention is superior to zinc oxide in improving the intestinal barrier function of piglets.

Table 6 Effect of zinc oxide substitution on cecal microflora of weaned piglets (log (copy 502956 number /g))

Com | Com | Com | Com | Com | Com

Obs parat | parat | parat | parat | parat | parat erva | Emb | Emb P ive ive ive ive ive ive tion | odim | odim S |v

Item exa | exa | exa | exa | exa | exa cont | ent 1 | ent2 E | al s mple | mple | mple | mple | mple | mple rol | grou | grou M | u 1 2 3 4 5 6 grou p p e grou | grou | grou | grou | grou | grou p p p p p p p

Tota 0.0. 11.5 | 11.6 | 11.5 | 11.4 | 11.5 | 11.5 | 11.4 | 11.6 | 11.5 1 1 | 9 bact | 21 78 09 97 55 41 34 04 87 | 48 eria 6 | 1 0. | 0.

Baci | 8.23 | 8.48 | 8.79 | 8.34 | 848 | 850 | 854 | 867 | 845 | 1 | 3 lus 2 8 9 3 3 4 9 5 7 5/2 0 | 8

Lact 0. | 0. oba | 6.54 | 7.32 | 7.45 | 6.85 | 6.95 | 7.13 | 6.68 | 7.24 | 7.16 | 1 | 0 cillu 3e 1a Qa abc 1 abc abc Abc 5ab 3ab 2 0

Ss 710

Esc 0. | 0. heri | 9.93 | 9.46 | 8.97 | 9.69 | 9.58 | 9.70 | 9.65 | 944 | 958 | 1 | O chia | 1°? 7ab 5b 38 3ab 18 0? 7ab 9% | 3/0 coli 515

Bifid 0. | 0. oba | 5.01 | 6.15 | 6.55 | 5.77 | 5.68 | 5.79 | 6.05 | 5.82 | 6.06 | 2 | 0 cteri 8b ab Ga 7ab gab 7ab ab ab 2ab 5 2 um 4 | 8

As shown in Table 6, compared with the observation control group, the number b502956

Lactobacillus in the cecum chyme of weaned piglets can be significantly increased by using the embodiment 1 group and embodiment 2 group of the present invention (P < 0.05). Compared with the observation control group, the number of Escherichia coli in the cecum chyme of weaned piglets can be significantly reduced and the number of

Bifidobacterium in the cecum chyme of weaned piglets can be increased (P < 0.05).

Compared with embodiment 1 group and embodiment 2 group, the number of

Lactobacillus in the cecum chyme of weaned piglets in comparative example 4 group decreases significantly (P < 0.05). Compared with the group of embodiment 2, the number of Escherichia coli in the cecum chyme of weaned piglets in the comparative example 1 group and comparative example 3 group increases significantly (P < 0.05).

There is no significant difference in the number of total bacteria of the cecum chyme and

Bacillus in cecum among the treatment groups (P > 0.05). To sum up, it shows that the feed additive of the invention can increase the number of beneficial bacteria, reduce the number of harmful bacteria, and is superior to zinc oxide in improving intestinal microbial composition.

To sum up, the feed additive can effectively improve the intestinal barrier function of piglets, maintain the microecological balance of piglets’ intestines, improve the nutrient digestibility of piglets, and finally improve the growth performance of piglets. The fee additive of the invention can be used as a zinc oxide substitute for production and application in the daily diet of wean piglets.

It needs to be explained that the technical effect of the invention is the result of the mutual cooperation and interaction of process steps and parameters, not the superposition of simple processes. The effect produced by the organic combination of each process far exceeds the superposition of the functions and effects of each single process, and it has better advancement and practicability.

The above are only embodiments of the present invention, which do not limit the patent scope of the present invention. All equivalent structural changes made by using the contents of the present specification, or directly or indirectly applied in other related technical fields, are equally included in the patent protection scope of the present invention.

LU502956

Claims (10)

CLAIMS LU502956

1. À piglet feed additive comprises 2-3 parts of yeast culture; 1-3 parts of organic acidifier; 0.1-0.2 part of plant extract; 0.5-1 part of probiotics; 2-4 parts of sodium humate;

0.3-0.5 part of antibacterial peptide; and 5-8 parts of carriers.

2. The piglet feed additive according to claim 1, wherein the organic acidifier is prepared by the following method: taking hawthorn powder, benzoic acid, fumaric acid and citric acid at a mass ratio of 2-3: 5: 1-1.5: 1-1.5 for later use; (1) putting hawthorn fruit into an extracting tank, boiling it for 2 hours with 10 times of water and the steam pressure of 1Mpa, filtering and separating to obtain the primary filtrate and filter residue; (2) boiling the primary filter residue again for 2 hours with 10 times of water and the steam pressure of 1Mpa, filtering and separating to obtain the secondary filtrate and filter residue; (3) boiling the secondary filter residue again for 1 hour with 5 times of water and the steam pressure is 1Mpa, filtering and separating to obtain the tertiary filtrate and filter residue, and discarding the filter residue; (4) mixing the primary filtrate, the secondary filtrate and the tertiary filtrate, and concentrating them to 1/4 of the original volume; (5) pouring the concentrated mixed filtrate into spray drier to obtain the hawthorn powder for later use; (6) pouring benzoic acid, fumaric acid and citric acid into a stirrer and stirring for 3 minutes at a stirring speed of 120 rpm to prepare the first mixed acidifier; (7) pouring hawthorn powder into a mixer, and mixing it with the first mixed acidifier for 8 minutes, with the stirring speed of 180 rpm, to make the finished product of organic acidifier.

3. The piglet feed additive according to claim 1, wherein the plant extract is prepared by the following method: taking cinnamaldehyde, carvacrol, thymol, capsaicin and curcumin at a mass ratio of 2-3:4-5:1-2:0.5-1:0.5-1 for later use; (1) adding cinnamaldehyde, carvacrol and thymol into a stirrer, and stirring for 3 minutes at a stirring speed of 120 rpm to prepare the first mixed plant extract;

(2) pouring capsaicin and curcumin into a stirrer, and stirring for 3 minutes at 14/502956 stirring speed of 120 rpm to prepare the second mixed plant extract; (3) pouring the first mixed plant extract and the second mixed plant extract into a stirrer, and stirring for 8 minutes at a stirring speed of 180 rpm to prepare the finished plant extract.

4. The piglet feed additive according to claim 1, wherein the probiotics are prepared by the following raw materials in mass ratio: the mass ratio of Bacillus subtilis to Bacillus licheniformis is 1-2:2-3.

5. The piglet feed additive according to claim 1, wherein the sodium humate is coated sodium humate; and the carrier is unite bran.

6. A method for preparing the piglet feed additive according to any one of claims 1 to comprises the following steps: (1) dividing the carriers into three parts and sodium humate into three parts; (2) mixing organic acidifier and antibacterial peptide to obtain the first mixture; (3) mixing the first mixture with the first part sodium humate to obtain the second mixture; (4) mixing the second mixture with the first part carrier to obtain the third mixture; (5) mixing the plant extract with probiotics to obtain the fourth mixture; (6) mixing the fourth mixture with the second part sodium humate to obtain the fifth mixture; (7) mixing the fifth mixture with the second carrier to obtain the sixth mixture; (8) mixing the yeast culture with the third part of sodium humate to obtain the seventh mixture; (9) mixing the third mixture, the sixth mixture and the seventh mixture with the third part carrier to obtain the piglet feed additive.

7. The method for preparing the piglet feed additive according to claim 6, wherein the mass ratio of the first part carrier, the second part carrier and the third part carrier is 3-5:1-3:4-7, and the mass ratio of the first part sodium humate, second part sodium humate and third part sodium humate is 3-6:1-3:4-6.

8. The method for preparing the piglet feed additive according to claim 6, wherein the mass ratio of the first part carrier, the second part carrier and the third part carrier 14/502956 4:2:6, and the mass ratio of the first part sodium humate, second part sodium humate and third part sodium humate is 5:2:5.

9. The piglet feed additive prepared according to any one of the preparation methods of claims 6 to 8.

10. The application of the piglet feed additive according to any one of claims 1-5 and 9, wherein the feed additive is added into the weaned piglet diet, and the added amount of the feed additive is 0.3-0.5% of the basic diet.