CN116491595A - Probiotic feed additive for improving growth performance of beef cattle and preparation method thereof - Google Patents

Probiotic feed additive for improving growth performance of beef cattle and preparation method thereof Download PDF

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Publication number
CN116491595A
CN116491595A CN202310677750.1A CN202310677750A CN116491595A CN 116491595 A CN116491595 A CN 116491595A CN 202310677750 A CN202310677750 A CN 202310677750A CN 116491595 A CN116491595 A CN 116491595A
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China
Prior art keywords
parts
beef cattle
cfu
probiotics
growth performance
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CN202310677750.1A
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Chinese (zh)
Inventor
朱伟
董建宝
郭建强
柳美玲
毕司英
杜燕
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Shandong Vocational Animal Science and Veterinary College
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Shandong Vocational Animal Science and Veterinary College
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Priority to CN202310677750.1A priority Critical patent/CN116491595A/en
Publication of CN116491595A publication Critical patent/CN116491595A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/10Feeding-stuffs specially adapted for particular animals for ruminants
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/33Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A23K10/38Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/24Compounds of alkaline earth metals, e.g. magnesium
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/30Oligoelements
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

The invention provides a probiotics feed additive for improving the growth performance of beef cattle and a preparation method thereof, belonging to the field of feeds, and the probiotics feed additive is prepared from the following raw materials; wherein the daily ration raw materials are prepared by mixing wild buckwheat rhizome, silage corns, ryegrass, bean pulp, wheat bran, cottonseed meal, DDGS, molasses, stone powder, salt and baking soda; the premix consists of soluble starch, xylo-oligosaccharide, calcium carbonate, sodium chloride, multi-ore, multi-dimensional and water; the probiotic preparation consists of bacillus licheniformis, bacillus subtilis, saccharomycetes, lactobacillus and bifidobacterium. The invention adds 5% -8% of probiotics preparation in the feed, and the bacillus licheniformis is more than or equal to 5.0×10 7 CFU/g, bacillus subtilis is more than or equal to 5.0X10 7 CFU/g, yeast not less than 1.0X10 8 CFU/g, lactobacillus not less than 5.0X10 7 CFU/g, bifidobacterium not less than 1.0X10 8 CFU/g, and the premix liquid is added, so that the growth performance of beef cattle can be remarkably improved.

Description

Probiotic feed additive for improving growth performance of beef cattle and preparation method thereof
Technical Field
The invention relates to the field of feed processing, in particular to a probiotic feed additive for improving the growth performance of beef cattle and a preparation method thereof.
Background
Antibiotics play a very important role in improving animal growth performance and preventing diseases, but have serious negative effects such as antibiotic residues, generation of drug-resistant strains, and the like. Along with implementation of feed end resistance in China, a novel tie-resistant product needs to be developed in the aspects of promoting animal growth and development and preventing diseases. At present, main tiepin products comprise additives such as microecological preparations, plant extracts, acidulants, chinese herbal medicines and the like, and the tiepin has good effects. Wherein, the microecological preparation product is mainly applied to the research of poultry, pigs, aquatic products and the like. The microecological preparation used in animal cultivation mainly comprises saccharomycetes, lactobacillus, clostridium butyricum, bifidobacterium, etc. The probiotics can be applied to animal production to effectively promote the growth and development of animals, improve intestinal health and enhance organism immunity and disease resistance. The use effect of probiotics is generally better than that of single probiotics. Therefore, the invention provides the probiotic feed additive for improving the growth performance of beef cattle.
Disclosure of Invention
Aiming at the prior art, the invention provides a probiotic feed additive for improving the growth performance of beef cattle and a preparation method thereof. The invention adds 5% -8% of probiotics preparation in the feed, and the bacillus licheniformis is more than or equal to 5.0×10 7 CFU/g, bacillus subtilis is more than or equal to 5.0X10 7 CFU/g, yeast not less than 1.0X10 8 CFU/g, lactobacillus not less than 5.0X10 7 CFU/g, bifidobacterium not less than 1.0X10 8 CFU/g, and the premix liquid is added, so that the growth performance of beef cattle can be remarkably improved.
The invention is realized by the following technical scheme: one aspect of the invention provides a probiotic feed additive for improving the growth performance of beef cattle, which is prepared from the following raw materials; wherein the daily ration raw materials are prepared by mixing wild buckwheat rhizome, silage corns, ryegrass, bean pulp, wheat bran, cottonseed meal, DDGS, molasses, stone powder, salt and baking soda; the premix consists of soluble starch, xylo-oligosaccharide, calcium carbonate, sodium chloride, multi-ore, multi-dimensional and water; the probiotic preparation consists of bacillus licheniformis, bacillus subtilis, saccharomycetes, lactobacillus and bifidobacterium.
Preferably, the daily ration raw material is prepared by mixing 8-10 parts of wild buckwheat rhizome, 20-30 parts of silage corn, 8-10 parts of ryegrass, 8-10 parts of bean pulp, 8-10 parts of wheat bran, 15-18 parts of cottonseed meal, 6-10 parts of DDGS, 6-10 parts of molasses, 6-10 parts of stone powder, 6-10 parts of salt and 6-10 parts of baking soda.
Preferably, the premix solution is composed of the following raw materials in parts by mass: 10 to 15 parts of soluble starch, 12 to 15 parts of xylo-oligosaccharide, 3 to 4 parts of calcium carbonate, 1 to 3 parts of sodium chloride, 0.8 to 1.2 parts of multi-mineral, 0.8 to 1.2 parts of multi-vitamin and 40 to 55 parts of water.
The premix provides VA3500U, VD600U, VE30U, fe80mg, cu20mg, na45mg, mn50mg and Se0.22mg for each kilogram of daily ration raw material.
Preferably, the probiotic preparation is prepared from bacillus licheniformis which is more than or equal to 5.0X10 7 CFU/g, bacillus subtilis is more than or equal to 5.0X10 7 CFU/g, yeast not less than 1.0X10 8 CFU/g, lactobacillus not less than 5.0X10 7 CFU/g, bifidobacterium not less than 1.0X10 8 CFU/g composition.
Preferably, the addition amount of the probiotics is 5% -8% of the daily ration raw material.
In another aspect, the invention provides a method for preparing a probiotic feed additive for improving beef cattle growth performance, comprising the following steps:
(1) Cutting rhizoma Fagopyri Dibotryis and ryegrass into short pieces, and mixing with silage corn crushed particles, bean pulp, cottonseed meal, wheat bran, DDGS (distillers grains with corn), molasses, stone powder, salt and sodium bicarbonate;
(2) And adding the premix and the probiotic preparation, dissolving the probiotics in the premix, spraying the dissolved probiotics on the daily ration raw materials, and fully stirring in a reaction way to uniformly mix the probiotics.
Preferably, the preparation method of the premix comprises the following steps: firstly, dissolving calcium carbonate and sodium chloride in water, then adding soluble starch, xylooligosaccharide, polymineral and multivitamin, heating to 55-65 ℃, and then preserving heat for 15-25 min.
The beneficial effects are that:
the invention discloses a probiotic feed additive for improving the growth performance of beef cattle, wherein 5% -8% of probiotic preparation is added into the feed, and the bacillus licheniformis is more than or equal to 5.0X10 7 CFU/g, bacillus subtilis is more than or equal to 5.0X10 7 CFU/g, yeast not less than 1.0X10 8 CFU/g, lactobacillus not less than 5.0X10 7 CFU/g, bifidobacterium not less than 1.0X10 8 CFU/g, and the premix liquid is added, so that the growth performance of beef cattle can be remarkably improved.
Drawings
For a clearer description of embodiments of the invention or of the solutions of the prior art, reference will be made to the accompanying drawings which are used in the description of embodiments or of the prior art, it being obvious to a person skilled in the art that other drawings can be obtained from these without inventive effort.
FIG. 1 is a graph showing the effect of different amounts of probiotics added to feed on beef cattle growth performance.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention.
It should be noted that the following embodiments and features in the embodiments may be combined with each other without conflict. It is also to be understood that the terminology used in the examples of the invention is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs and to which this invention belongs, and any method, apparatus, or material of the prior art similar or equivalent to the methods, apparatus, or materials described in the examples of this invention may be used to practice the invention.
Where numerical ranges are provided in the examples, it is understood that unless otherwise stated herein, both endpoints of each numerical range and any number between the two endpoints are significant both in the numerical range. The test methods in the following examples, in which specific conditions are not noted, are generally conducted under conventional conditions or under conditions recommended by the respective manufacturers. The materials or reagents required in the examples below are commercially available unless otherwise specified.
The following examples relate to a probiotic feed additive for improving beef cattle growth performance.
Example 1 a probiotic feed additive for improving beef cattle growth performance is prepared from the following raw materials, a ration raw material, a premix and probiotics; wherein the daily ration raw materials are prepared by mixing wild buckwheat rhizome, silage corns, ryegrass, bean pulp, wheat bran, cottonseed meal, DDGS, molasses, stone powder, salt and baking soda; the premix consists of soluble starch, xylo-oligosaccharide, calcium carbonate, sodium chloride, multi-ore, multi-dimensional and water; the probiotic preparation consists of bacillus licheniformis, bacillus subtilis, saccharomycetes, lactobacillus and bifidobacterium.
The daily ration raw material is prepared by mixing 8-10 parts of wild buckwheat, 20-30 parts of silage corns, 8-10 parts of ryegrass, 8-10 parts of bean pulp, 8-10 parts of wheat bran, 15-18 parts of cottonseed meal, 6-10 parts of DDGS, 6-10 parts of molasses, 6-10 parts of stone powder, 6-10 parts of salt and 6-10 parts of baking soda.
The premix solution comprises the following raw materials in parts by mass: 10 to 15 parts of soluble starch, 12 to 15 parts of xylo-oligosaccharide, 3 to 4 parts of calcium carbonate, 1 to 3 parts of sodium chloride, 0.8 to 1.2 parts of multi-mineral, 0.8 to 1.2 parts of multi-vitamin and 40 to 55 parts of water.
The premix provides VA3500U, VD600U, VE30U, fe80mg, cu20mg, na45mg, mn50mg and Se0.22mg for each kilogram of daily ration raw material.
The probiotic preparation is prepared from bacillus licheniformis which is more than or equal to 5.0X10 7 CFU/g, bacillus subtilis is more than or equal to 5.0X10 7 CFU/g, yeast not less than 1.0X10 8 CFU/g, lactobacillus not less than 5.0X10 7 CFU/g, bifidobacterium not less than 1.0X10 8 CFU/g composition.
The addition amount of the probiotics is 5% -8% of the daily ration raw material.
Embodiment 2, a method for preparing a probiotic feed additive for improving beef cattle growth performance, comprises the following steps:
(1) Cutting rhizoma Fagopyri Dibotryis and ryegrass into short pieces, and mixing with silage corn crushed particles, bean pulp, cottonseed meal, wheat bran, DDGS (distillers grains with corn), molasses, stone powder, salt and sodium bicarbonate;
(2) And adding the premix and the probiotic preparation, dissolving the probiotics in the premix, spraying the dissolved probiotics on the daily ration raw materials, and fully stirring in a reaction way to uniformly mix the probiotics.
The preparation method of the premix comprises the following steps: firstly, dissolving calcium carbonate and sodium chloride in water, then adding soluble starch, xylooligosaccharide, polymineral and multivitamin, heating to 55-65 ℃, and then preserving heat for 15-25 min.
And (3) experimental verification:
the probiotics used in the test were purchased from Fushite Biotechnology Co.
The test selects 40 beef cattle with similar weight and good body condition, and the test beef cattle are divided into 4 groups of 5 replicates according to the single factor random grouping principle, and 2 beef cattle are replicated each. The control group beef cattle are not fed with probiotic feed, and the test 1-3 groups beef cattle are respectively fed with feed containing 4%, 5% and 8% of probiotics. The test period is 9 weeks, wherein the pre-feeding period is 1 week, the formal test period is 8 weeks, and the growth performance indexes of the beef cattle in each group are counted after the test.
And (3) feeding management: before the test, the cowshed is disinfected, ventilated and dried, and then subjected to preliminary experiments and formal experiments. All test beef cattle were kept in a co-cowshed and the test beef cattle were free to eat and drink water. The test cattle are periodically insect-repellent and immunized, and the procedure is carried out according to the conventional management of beef cattle farms.
Detecting items: before the start of the test and after the end of the test, the beef cattle were fasted for 12 hours, and the initial body mass and the end body mass of the test were weighed. The feed intake of each group of test beef cattle is accurately recorded every day during the test period, and the average daily gain of the beef cattle is calculated according to the data.
Average daily gain= (final body mass-initial body mass)/test days (1)
Average daily feed intake = total feed consumption per component beef cattle tested period/days tested (2)
Feed weight ratio = average daily feed intake/average daily gain (3)
Data processing and analysis: the data collected by the test are firstly subjected to simple statistical arrangement by a table, then subjected to single factor analysis of variance by adopting SPSS24.0, subjected to multiple comparison of the data among groups by a Duncan's method, presented by average number +/-standard deviation, wherein P <0.05 represents significant difference of the data among groups, and P <0.01 represents extremely significant difference of the data among groups.
The effect of different doses of probiotics added into the feed on the growth performance of beef cattle is shown in figure 1. As can be seen from the data in fig. 1, adding a certain amount of probiotics to beef cattle ration can significantly improve the average daily gain and significantly reduce the feed-weight ratio. Wherein, the average daily gain of beef cattle in 5% of probiotics group and 8% of probiotics group is obviously higher than that in control group, which is respectively improved by 20.00% and 21.33% (P < 0.05), the average daily gain of beef cattle in 4% of probiotics group is easier than that in control group, but the difference is not obvious (P > 0.05); the feed weight ratio of the 5% probiotic group and the 8% probiotic group beef cattle is extremely lower than that of the control group and the 4% probiotic group, which are respectively reduced by 16.59% and 16.59% (P < 0.01) compared with the control group, and 11.46% P < 0.01) compared with the 4% probiotic group; the probiotics at different doses had no significant effect on the average daily feed intake of beef cattle (P > 0.05).
Note that the same column data shoulder indicates significant differences (P < 0.05) with lower case letter differences, and the upper case letter differences represent very significant differences (P < 0.01), with the same letter or no letter indicating no significant differences (P > 0.05).
In the test, the average daily gain of beef cattle in 5% of probiotics group and 8% of probiotics group is obviously higher than that in the control group, and is respectively improved by 20.00% and 21.33% compared with the control group; the feed weight ratio of the 5% probiotics group and the 8% probiotics group beef cattle is extremely lower than that of the control group and the 4% probiotics group, the feed weight ratio is reduced by 16.59% and 16.59% respectively compared with the control group, and the feed weight ratio is reduced by 11.46% and 11.46% respectively compared with the 4% probiotics group, and the result shows that the growth performance of the beef cattle can be obviously improved by feeding 5% probiotics.
While the invention has been described with reference to the preferred embodiments, it is not limited thereto, and various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.
It is to be understood that, based on the several embodiments provided in the present application, those skilled in the art may combine, split, reorganize, etc. the embodiments of the present application to obtain other embodiments, where none of the embodiments exceed the protection scope of the present application.
The foregoing detailed description of the embodiments of the present application has further described the objects, technical solutions and advantageous effects thereof, and it should be understood that the foregoing is merely a specific implementation of the embodiments of the present application, and is not intended to limit the scope of the embodiments of the present application, and any modifications, equivalent substitutions, improvements, etc. made on the basis of the technical solutions of the embodiments of the present application should be included in the scope of the embodiments of the present application.

Claims (8)

1. A probiotic feed additive for improving the growth performance of beef cattle is characterized in that: is prepared from the following raw materials including daily ration raw material, premix solution and probiotics; wherein the daily ration raw materials are prepared by mixing wild buckwheat rhizome, silage corns, ryegrass, bean pulp, wheat bran, cottonseed meal, DDGS, molasses, stone powder, salt and baking soda; the premix consists of soluble starch, xylo-oligosaccharide, calcium carbonate, sodium chloride, multi-ore, multi-dimensional and water; the probiotic preparation consists of bacillus licheniformis, bacillus subtilis, saccharomycetes, lactobacillus and bifidobacterium.
2. A probiotic feed additive for improving beef cattle growth performance according to claim 1, characterized in that: the daily ration raw material is prepared by mixing 8-10 parts of wild buckwheat, 20-30 parts of silage corns, 8-10 parts of ryegrass, 8-10 parts of bean pulp, 8-10 parts of wheat bran, 15-18 parts of cottonseed meal, 6-10 parts of DDGS, 6-10 parts of molasses, 6-10 parts of stone powder, 6-10 parts of salt and 6-10 parts of baking soda.
3. A probiotic feed additive for improving beef cattle growth performance according to claim 1, characterized in that: the premix solution comprises the following raw materials in parts by mass: 10 to 15 parts of soluble starch, 12 to 15 parts of xylo-oligosaccharide, 3 to 4 parts of calcium carbonate, 1 to 3 parts of sodium chloride, 0.8 to 1.2 parts of multi-mineral, 0.8 to 1.2 parts of multi-vitamin and 40 to 55 parts of water.
4. A probiotic feed additive for improving beef cattle growth performance according to claim 1, characterized in that: the premix provides VA3500U, VD600U, VE30U, fe80mg, cu20mg, na45mg, mn50mg and Se0.22mg for each kilogram of daily ration raw material.
5. A probiotic feed additive for improving beef cattle growth performance according to claim 1, characterized in that: the probiotic preparation is prepared from bacillus licheniformis which is more than or equal to 5.0X10 7 CFU/g, bacillus subtilis is more than or equal to 5.0X10 7 CFU/g, yeast not less than 1.0X10 8 CFU/g, lactobacillus not less than 5.0X10 7 CFU/g, bifidobacterium not less than 1.0X10 8 CFU/g composition.
6. A probiotic feed additive for improving beef cattle growth performance according to claim 1, characterized in that: the addition amount of the probiotics is 5% -8% of the daily ration raw material.
7. The preparation method of the probiotic feed additive for improving the growth performance of beef cattle is characterized by comprising the following steps of:
(1) Cutting rhizoma Fagopyri Dibotryis and ryegrass into short pieces, and mixing with silage corn crushed particles, bean pulp, cottonseed meal, wheat bran, DDGS, molasses, stone powder, salt and sodium bicarbonate;
(2) And adding the premix and the probiotic preparation, dissolving the probiotics in the premix, spraying the dissolved probiotics on the daily ration raw materials, and fully stirring in a reaction way to uniformly mix the probiotics.
8. The method according to claim 7, wherein: the preparation method of the premix comprises the following steps: firstly, dissolving calcium carbonate and sodium chloride in water, then adding soluble starch, xylooligosaccharide, polymineral and multivitamin, heating to 55-65 ℃, and then preserving heat for 15-25 min.
CN202310677750.1A 2023-06-09 2023-06-09 Probiotic feed additive for improving growth performance of beef cattle and preparation method thereof Pending CN116491595A (en)

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