KR940018097A - 내독소 활성의 저해방법 - Google Patents

내독소 활성의 저해방법 Download PDF

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Publication number
KR940018097A
KR940018097A KR1019940000977A KR19940000977A KR940018097A KR 940018097 A KR940018097 A KR 940018097A KR 1019940000977 A KR1019940000977 A KR 1019940000977A KR 19940000977 A KR19940000977 A KR 19940000977A KR 940018097 A KR940018097 A KR 940018097A
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South Korea
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endotoxin
surfactant
peptide
inhibits
reaction
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KR1019940000977A
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English (en)
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KR100255993B1 (ko
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마사까즈 쓰찌야
가즈아끼 하라다
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다나까 모또아끼
와꼬쥰야꾸고오교 가부시끼가이샤
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/50—Cyclic peptides containing at least one abnormal peptide link
    • C07K7/54—Cyclic peptides containing at least one abnormal peptide link with at least one abnormal peptide link in the ring
    • C07K7/60—Cyclic peptides containing at least one abnormal peptide link with at least one abnormal peptide link in the ring the cyclisation occurring through the 4-amino group of 2,4-diamino-butanoic acid
    • C07K7/62—Polymyxins; Related peptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria

Abstract

본 발명은 내독소(ET)의 활성을 저해하기 위하여 ET와 결합하는 성질을 가진 펩티드 유도체 또는 단백질 및 하나 이상의 계면활성제를 ET를 함유하는 시료와 접촉시킴으로서 ET의 활성을 저해시킬 수 있으며, 심지어 ET활성을 가지는 ET 유사물질이 처리한 시료중에 존재해 있을 때에도 이 물질을 ET의 효과없이도 측정할 수 있는 내독소 저해 방법에 관한 것이다.

Description

내독소 활성의 저해방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제 1 도는 실시예 1 에서 수득하였고, 혈장내 내독소(이하 "ET"라 약칭함)(대장균 055:B5 LPS) 측정에 대한 계면활성제 농도의 효과를 보이는 그래프이다' 제2도는 실시예 1 에서 수득하였고, 혈장내 내독소(대장균 055:B5 LPS) 측정에 대한 폴리믹신B(이하 "Px B"라 약칭함) 농도의 효과를 보이는 그래프이다.

Claims (17)

  1. 내독소의 활성을 저해시키기 위하여 내독소에 결합하는 성질이 있으며, 펩티드 유도체와 단백질로부터 선택된 내독소를 저해하는 펩티드 및 하나 이상의 계면활성제를 내독소 함유 시료와 접촉시키는 것을 포함하는 내독소 활성의 저해 방법.
  2. 제 1 항에 있어서, 열처리를 동시에 수행하는 방법.
  3. 제 1 항에 있어서, 내독소를 저해하는 펩티드 및 계면활성제가 시료와 접촉한 이후에 시료를 열처리하는 방법.
  4. 제 1 항에 있어서, 계면활성제의 농도는 0.005~5.0w/v%이고, 내독소를 저해하는 펩티드의 농도는 0.00001~0.1w/v%인 방법.
  5. 제 2 항에 있어서, 60~220℃에서 3~60분동안 가열시키는 열처리를 수행하는 방법.
  6. 제 1 항에 있어서, 내독소를 저해하는 펩티드가 폴리믹신인 방법.
  7. 제 1 항에 있어서, 계면활성제가 비이온성 계면활성제 또는 양성(兩性) 계면활성제인 방법.
  8. 상기 제 1 항의 방법으로 처리한 시료를 참게의 혈구 용해물과 반응시키고, 상기 반응에 의해 일어난 효소 활성화 반응으로 활성화된 효소의 활성 또는 상기 반응에 의해 일어난 젤화 반응에 기인하는 반응 용액의 탁도 변화나 젤화 정도를 측정기구를 사용하거나 나안으로 측정하는 것을 포함하며, 시료중에 존재하고, 참게의 혈구 용해물과 반응하여 효소 활성화 반응 또는 젤화 반응을 일으키는 성질을 가진 하나 이상의 내독소 유사물질을 측정하는 방법.
  9. 제 8 항에 있어서, 내독소 유사물질이 (1→3)-β-D-글루칸 및/또는 그 유도체인 방법.
  10. 참게의 혈구 용해물과 반응하지 못하고, 참게의 혈구 용해물과 내독소 유사물질과의 반응을 저해 및 촉진시킬 수도 없는 용액으로, 내독소를 저해하는 펩티드 및 하나 이상의 계면활성제를 함유하는, 내독소의 활성을 저해하는 전처리 수용액.
  11. 제10항에 있어서, 계면활성제 농도는 0.01~10.0w/v%이고 내독소를 저해하는 펩티드의 농도는 0.00002~0.2w/v%인 전처리 수용액.
  12. 제10항에 있어서, 내독소를 저해하는 펩티드가 폴리믹신인 전처리 수용액.
  13. 제10항에 있어서, 계면활성제가 비이온성 계면활성제 또는 양성 계면활성제인 전처리 수용액.
  14. 내독소를 저해하는 펩티드, 하나 이상의 계면활성제 및 AL 용액을 함유하는 하나 이상의 내독소 유사물질을 측정하는 시약.
  15. 제14항에 있어서, 내독소를 저해하는 펩티드가 폴리믹신인 시약.
  16. 제14항에 있어서, 계면활성제가 비이온성 계면활성제 또는 양성 계면활성제인 시약.
  17. 제14항에 있어서, 내독소 유사물질이 (1→3)-β-D-글루칸 및/또는 그 유도체인 시약.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019940000977A 1993-01-21 1994-01-19 내독소 활성의 저해방법 KR100255993B1 (ko)

Applications Claiming Priority (2)

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JP2615993 1993-01-21
JP93-26159 1993-01-21

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KR940018097A true KR940018097A (ko) 1994-08-16
KR100255993B1 KR100255993B1 (ko) 2000-05-01

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US (3) US5750500A (ko)
EP (1) EP0608982B1 (ko)
KR (1) KR100255993B1 (ko)
CN (2) CN1120370C (ko)
CA (1) CA2112776C (ko)
DE (1) DE69405594T2 (ko)
ES (1) ES2107124T3 (ko)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2112776C (en) * 1993-01-21 2002-11-12 Masakazu Tsuchiya Process for inhibiting activity of endotoxin
AU2001252219B2 (en) * 2000-03-24 2006-02-09 Societe Des Produits Nestle S.A. Beta-glucans from filamentous fungi
JPWO2002101836A1 (ja) * 2001-06-12 2004-09-30 株式会社日立製作所 半導体装置およびその製造方法
CA2506236C (en) * 2002-11-18 2018-07-17 Vicuron Pharmaceuticals Inc. Compositions and uses of dalbavancin for treatment of bacterial infections
WO2005010207A2 (en) * 2003-03-17 2005-02-03 Charles River Laboratories, Inc. Methods and compositions for the detection of microbial contaminants
US20060004185A1 (en) * 2004-07-01 2006-01-05 Leese Richard A Peptide antibiotics and peptide intermediates for their prepartion
WO2007078268A2 (en) * 2004-12-02 2007-07-12 Charles River Laboratories, Inc. Methods and compositions for the detection and/or quantification of gram positive bacterial contaminants
EP1836492B1 (en) 2005-01-13 2008-12-31 Charles River Laboratories, Inc. Method for classifying a microorganism in a biological sample
WO2009137244A1 (en) * 2008-04-15 2009-11-12 Charles River Laboratories, Inc. Cartridge and method for sample analysis
WO2010075416A1 (en) 2008-12-23 2010-07-01 Biosource Pharm, Inc. Antibiotic compositions for the treatment of gram negative infections
CA2766288C (en) 2009-06-26 2020-05-05 Charles River Laboratories, Inc. Heat-treated limulus amebocyte lysates
US8415307B1 (en) 2010-06-23 2013-04-09 Biosource Pharm, Inc. Antibiotic compositions for the treatment of gram negative infections
US20130078665A1 (en) * 2012-07-18 2013-03-28 Deepika Bodapati Test strip, a test kit and a method for detection of endotoxin in food
CN103592442B (zh) * 2013-10-29 2016-01-20 王明丽 一种检测重组猪干扰素α1冻干粉针剂中细菌内毒素含量的方法
CN108387739A (zh) * 2018-01-23 2018-08-10 福州启新生物技术有限公司 一种特异性检测血清中真菌的试剂及其制备方法
CN109142760B (zh) * 2018-07-04 2021-02-02 中国农业大学 一种快速准确检测饲料中粘杆菌素的试剂盒

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5648897A (en) * 1979-09-25 1981-05-02 Nitsusui Seiyaku Kk Selective isolation medium for cholera bacillus
US4276050A (en) * 1980-01-10 1981-06-30 Abbott Laboratories Method for systemic endotoxin detection
US4322217A (en) * 1980-06-27 1982-03-30 Mallinckrodt, Inc. Process for preparing Limulus lysate
JPS5813519A (ja) * 1981-07-16 1983-01-26 Seikagaku Kogyo Co Ltd エンドトキシン吸着材及びそれを用いるエンドトキシンの除去方法
JPS5967210A (ja) * 1982-10-09 1984-04-16 Nissan Chem Ind Ltd 線虫防除剤
JPS60184100A (ja) * 1984-03-02 1985-09-19 Toray Ind Inc ポリミキシンの固定化方法
ATE188777T1 (de) * 1986-12-03 2000-01-15 Wako Pure Chem Ind Ltd Verfahren zum sammeln von insekten- körperflussigkeiten
US4803314A (en) * 1986-12-22 1989-02-07 Carlingswitch, Inc. Momentary rotary switch
DK255887D0 (da) * 1987-05-20 1987-05-20 Claus Koch Immunoassay
CA1337781C (en) * 1987-08-21 1995-12-19 Takanori Nakamura Polypeptide from horseshoe crab exhibiting affinity for lipopolysaccharide and method of preparation
US4808314A (en) * 1987-09-18 1989-02-28 Scripps Clinic And Research Foundation Method for reducing bacterial endotoxin contamination in solutions of macromolecules
JPH0715474B2 (ja) * 1988-02-27 1995-02-22 和光純薬工業株式会社 エンドトキシンの測定法
JPH0711523B2 (ja) * 1988-03-16 1995-02-08 和光純薬工業株式会社 試薬の調製方法
US5594113A (en) * 1988-06-23 1997-01-14 Associates Of Cape Cod, Inc. Endotoxin binding and neutralizing protein and uses thereof
JP2688773B2 (ja) * 1988-11-24 1997-12-10 和光純薬工業株式会社 エンドトキシンの不活化方法
JP2701916B2 (ja) * 1989-02-08 1998-01-21 マルハ株式会社 β―グルカンに対する特異性の高いアメボサイト・ライセート及びその調製方法
US5234912A (en) * 1989-02-14 1993-08-10 Incyte Pharmaceuticals, Inc. Pharmaceutical compositions comprising recombinant BPI proteins and a lipid carrier and uses thereof
US5177059A (en) * 1989-11-15 1993-01-05 Sandoz Ltd. Polymyxin B conjugates
JP3085310B2 (ja) * 1990-04-26 2000-09-04 日本バイリーン株式会社 微生物除去材
CA2037727A1 (en) * 1991-03-07 1992-09-08 Burton W. Blais Removing lps pyrogen from aqueous solution
JP2944709B2 (ja) * 1990-06-21 1999-09-06 生化学工業株式会社 （１→３）―β―Ｄ―グルカンの測定剤
US5198339A (en) * 1990-07-13 1993-03-30 Board Of Regents, The University Of Texas System Method for detection of gram-negative bacterial lipopolysaccharides in biological fluids
DE69221453T2 (de) * 1991-12-24 1998-02-19 Wako Pure Chem Ind Ltd Verfahren zum Extrahieren von Endotoxinen
EP0549102B1 (en) * 1991-12-25 1996-07-03 Maruha Corporation Beta-glucans detection reagents and methods of detecting beta-glucans
US5552294A (en) * 1992-10-20 1996-09-03 Children's Medical Center Corporation Rapid detection of virulence-associated factors
CA2112776C (en) * 1993-01-21 2002-11-12 Masakazu Tsuchiya Process for inhibiting activity of endotoxin

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Publication number Publication date
US5750500A (en) 1998-05-12
KR100255993B1 (ko) 2000-05-01
ES2107124T3 (es) 1997-11-16
CA2112776A1 (en) 1994-07-22
EP0608982B1 (en) 1997-09-17
DE69405594T2 (de) 1998-03-19
EP0608982A3 (en) 1995-04-19
CN1120370C (zh) 2003-09-03
US5616557A (en) 1997-04-01
CN1109598A (zh) 1995-10-04
DE69405594D1 (de) 1997-10-23
CN1379248A (zh) 2002-11-13
US5622833A (en) 1997-04-22
CA2112776C (en) 2002-11-12
EP0608982A2 (en) 1994-08-03

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