KR910002893A - 안넥신의 정제방법 - Google Patents

안넥신의 정제방법 Download PDF

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Publication number
KR910002893A
KR910002893A KR1019900010697A KR900010697A KR910002893A KR 910002893 A KR910002893 A KR 910002893A KR 1019900010697 A KR1019900010697 A KR 1019900010697A KR 900010697 A KR900010697 A KR 900010697A KR 910002893 A KR910002893 A KR 910002893A
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South Korea
Prior art keywords
annexin
added
chelating agent
cell residue
annexines
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KR1019900010697A
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English (en)
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KR0159275B1 (ko
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로이텔링스페르거 크리스티안
보도 게르하르트
Original Assignee
디터 라우딘, 루돌프 호프만
베링거 인겔하임 인터나쇼날 게엠베하
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Publication of KR910002893A publication Critical patent/KR910002893A/ko
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Publication of KR0159275B1 publication Critical patent/KR0159275B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4721—Lipocortins

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Detergent Compositions (AREA)
  • Amplifiers (AREA)
  • Bidet-Like Cleaning Device And Other Flush Toilet Accessories (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Steroid Compounds (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)

Abstract

내용 없음.

Description

안넥신의 정제방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 페닐-20 세파로즈 급속 유동 크로마토그래피의 용출 프로파일(←→=VAC-풀(fool))이다. 제2도는 Q-세파로즈 급속 유동 크로마토그래피의 용출 프로파일(←→=VAC-풀)이다.

Claims (15)

  1. a) 균질화된 세포물질의 pH값을 8.0 내지 10.0으로 조절하고, b) Ca2+, Cd2+, Zn2+, Mn2+및 Co2+중에서 선택된 하나 이상의 이가 양이온을 가하며, c) 인지질을 가하고, d) 불용성 세포 잔사를 세척하여 용해성 조성물을 제거하며, c) 킬레이트화제를 사용하여 안넥신을 세포잔사로부터 추출하는 단계를 특징를 하여, 안넥신을 정제하는 방법.
  2. 제1항에 있어서, 이온성 그룹을 가진 수지를 단계 (e) 이후 가하고 형성된 침전물을 분리하는 방법.
  3. 제2항에 있어서, 수지를 강한 양이온성 그룹으로 작용화시키는 방법.
  4. 제1항 또는 2항에 있어서, 단계(e)에서 수득한 안넥신을 이전에 공지된 방법으로 단백질 침전 및 연속 단백질 정제 단계에 의해 추가로 정제하는 방법.
  5. 제1항 또는 2항에 있어서, 단계(a)에서 pH를 9.0으로 조절시킨 방법.
  6. 제1항 내지 3항중 어느 한 항에 있어서, ca2+을 단계(b)에서 이가 양이온으로서 가하는 방법.
  7. 제6항에 있어서, Ca2+및 Zn2+를 단계(b)에서 이가 양이온으로서 가하는 방법.
  8. 제1항 내지 7항중 어느 한 항에 있어서, 레시틴을 단계(c)에서 인지질로서 가하는 방법.
  9. 제1항 내지 8항중 어느 한 항에 있어서, 단계(b) 및 (c)를 실질적으로 동시에 수행하는 방법.
  10. 제1항 내지 9항중 어느 한 항에 있어서, EDTA를 단계(e)에서 킬레이트화제로서 사용하는 방법.
  11. 제1항 내지 10항중 어느 한 항에 있어서, 세포를 균질화 및 단계(a) 이전에 비활성시키는 방법.
  12. 제11항에 있어서, 세포를 벤젠, 톨루엔, 페놀, 크실렌 및 크레졸 중에서 선택된 제제에 의해 비활성시키는 방법.
  13. 제12항에 있어서, m-크레졸을 비활성화제로서 사용하는 방법.
  14. 제1항 내지 13항중 어느 한 항에 있어서, 정제할 안넥신이 VAC 또는 이의 유사체인 방법.
  15. 제1항 내지 14항중 어느 한 항에 따른 방법으로 수득한 안넥신.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019900010697A 1989-07-15 1990-07-14 안넥신의 정제 방법 KR0159275B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DEP3923501.7 1989-07-15
DE3923501 1989-07-15
DEP39235017 1989-07-15

Publications (2)

Publication Number Publication Date
KR910002893A true KR910002893A (ko) 1991-02-26
KR0159275B1 KR0159275B1 (ko) 1998-11-16

Family

ID=6385155

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KR1019900010697A KR0159275B1 (ko) 1989-07-15 1990-07-14 안넥신의 정제 방법

Country Status (18)

Country Link
US (1) US5258497A (ko)
EP (1) EP0409053B1 (ko)
JP (1) JPH03175992A (ko)
KR (1) KR0159275B1 (ko)
AT (1) ATE95189T1 (ko)
AU (1) AU628604B2 (ko)
CA (1) CA2021147A1 (ko)
DD (1) DD297977A5 (ko)
DE (2) DE59002895D1 (ko)
DK (1) DK0409053T3 (ko)
ES (1) ES2045669T3 (ko)
FI (1) FI95136C (ko)
IE (1) IE65195B1 (ko)
IL (1) IL95082A (ko)
NO (1) NO177787C (ko)
NZ (1) NZ234492A (ko)
PT (1) PT94674B (ko)
ZA (1) ZA905490B (ko)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE89169T1 (de) * 1989-07-15 1993-05-15 Boehringer Ingelheim Int Antikoagulanz enthaltendes mittel.
US5627036A (en) * 1989-12-27 1997-05-06 Boehringer Ingelheim International Gmbh Use of an anticoagulant as a diagnostic agent
DE9110757U1 (de) * 1991-08-30 1992-02-13 Klein, Rainer, 5840 Schwerte Integriert-optischer Stoffsensor
WO1993006230A1 (en) * 1991-09-23 1993-04-01 The General Hospital Corporation Annexin xi
US5968477A (en) 1994-01-24 1999-10-19 Neorx Corporation Radiolabeled annexin conjugates with hexose and a chelator
JP3664727B2 (ja) * 1994-01-24 2005-06-29 ネオルクス コーポレイション 放射標識したアネキシン
US20030220233A1 (en) 1994-01-24 2003-11-27 Neorx Corporation Radiolabeled annexins
DE69533366T2 (de) * 1994-12-07 2005-08-04 Neorx Corp., Seattle Radioaktivmarkierte annexin-galaktose-cluster konjugate
US6204035B1 (en) 1996-04-02 2001-03-20 The Blood Center Research Foundation Methods and compositions to alter the cell surface expression of phosphatidylserine and other clot-promoting plasma membrane phospholipids
US6172210B1 (en) 1996-04-02 2001-01-09 Blood Center Research Foundation DNA encoding phospholipid scramblase
KR100841599B1 (ko) * 2000-07-21 2008-06-26 자이단호진 가가쿠오요비겟세이료호겐쿠쇼 칼슘 이온-결합 단백질의 정제 방법
US20050222030A1 (en) * 2001-02-21 2005-10-06 Anthony Allison Modified annexin proteins and methods for preventing thrombosis
US20090291086A1 (en) * 2001-02-21 2009-11-26 Alavita Pharmaceuticals, Inc. Compositions and Methods for Treating Cerebral Thrombosis and Global Cerebral Ischemia
US7635680B2 (en) * 2001-02-21 2009-12-22 Alavita Pharmaceuticals, Inc. Attenuation of reperfusion injury
US7635676B2 (en) * 2001-02-21 2009-12-22 Alavita Pharmaccuticals, Inc. Modified annexin proteins and methods for their use in organ transplantation
ES2286247T3 (es) * 2001-02-21 2007-12-01 Alavita Pharmaceuticals, Inc. Proteinas anexinicas modificacdas y tratamiento de la trombosis.
US7645739B2 (en) * 2001-02-21 2010-01-12 Alavita Pharmaceuticals, Inc. Modified annexin compositions and methods of using same
DE10162434A1 (de) * 2001-12-18 2003-09-25 November Ag Molekulare Medizin Expressionsvektor und Verfahren zur Herstellung eines Expressionsvektors
AU2003237870A1 (en) * 2002-06-07 2003-12-22 The Brigham & Women's Hospital, Inc. Method and composition for inhibing or slowing blood coagulation
WO2005005954A2 (en) * 2003-06-30 2005-01-20 The Brigham & Women's Hospital, Inc. Method, composition and kit for detecting phosphatidylserine (ps) on cell membranes
KR102597544B1 (ko) * 2021-04-02 2023-11-03 주식회사 디에스시동탄 시트용 서포트 구동부 및 이를 포함하는 서포트 어셈블리

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* Cited by examiner, † Cited by third party
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DE3315000A1 (de) * 1983-04-26 1984-10-31 Behringwerke Ag, 3550 Marburg Gewebeprotein pp(pfeil abwaerts)4(pfeil abwaerts), verfahren zu seiner gewinnung sowie seine verwendung
KR930007434B1 (ko) * 1984-09-21 1993-08-10 베링거 잉겔하임 인터내셔날 게엠베하 혈액응고 억제 단백질 제조방법
US4683294A (en) * 1985-04-03 1987-07-28 Smith Kline Rit, S.A. Process for the extraction and purification of proteins from culture media producing them
US4937324A (en) * 1987-02-06 1990-06-26 Zymogenetics, Inc. Chromatographic purification of human proteins having anticoagulant and anti-inflammatory activity

Also Published As

Publication number Publication date
IE902563A1 (en) 1991-02-27
NO903149D0 (no) 1990-07-13
NO903149L (no) 1991-01-16
PT94674B (pt) 1997-03-31
KR0159275B1 (ko) 1998-11-16
CA2021147A1 (en) 1991-01-16
DE59002895D1 (de) 1993-11-04
IL95082A0 (en) 1991-06-10
EP0409053B1 (de) 1993-09-29
US5258497A (en) 1993-11-02
AU628604B2 (en) 1992-09-17
IE65195B1 (en) 1995-10-04
DD297977A5 (de) 1992-01-30
FI903508A0 (fi) 1990-07-11
ATE95189T1 (de) 1993-10-15
NO177787C (no) 1995-11-22
AU5901590A (en) 1991-01-17
NO177787B (no) 1995-08-14
EP0409053A1 (de) 1991-01-23
ZA905490B (en) 1992-03-25
IL95082A (en) 1995-03-30
FI95136C (fi) 1995-12-27
NZ234492A (en) 1991-10-25
ES2045669T3 (es) 1994-01-16
PT94674A (pt) 1991-04-18
DK0409053T3 (da) 1993-11-08
DE4021979A1 (de) 1991-01-31
JPH03175992A (ja) 1991-07-31
FI95136B (fi) 1995-09-15

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