KR850001538A - 항생물질 sb 22484의 제조방법 - Google Patents
항생물질 sb 22484의 제조방법 Download PDFInfo
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- KR850001538A KR850001538A KR1019840005335A KR840005335A KR850001538A KR 850001538 A KR850001538 A KR 850001538A KR 1019840005335 A KR1019840005335 A KR 1019840005335A KR 840005335 A KR840005335 A KR 840005335A KR 850001538 A KR850001538 A KR 850001538A
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- Prior art keywords
- antibiotic
- nujol
- antibiotics
- factor
- factors
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- 230000003115 biocidal effect Effects 0.000 title claims description 13
- 238000000034 method Methods 0.000 title claims 6
- 238000002360 preparation method Methods 0.000 title claims 2
- 238000000862 absorption spectrum Methods 0.000 claims description 4
- 238000000655 nuclear magnetic resonance spectrum Methods 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 4
- 239000003242 anti bacterial agent Substances 0.000 claims 4
- 229940088710 antibiotic agent Drugs 0.000 claims 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims 3
- 241000187180 Streptomyces sp. Species 0.000 claims 3
- 238000000855 fermentation Methods 0.000 claims 3
- 230000004151 fermentation Effects 0.000 claims 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 2
- 238000010521 absorption reaction Methods 0.000 claims 2
- 239000002253 acid Substances 0.000 claims 2
- 229910052799 carbon Inorganic materials 0.000 claims 2
- 150000002148 esters Chemical class 0.000 claims 2
- 238000002955 isolation Methods 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 229910052757 nitrogen Inorganic materials 0.000 claims 2
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims 2
- 238000011084 recovery Methods 0.000 claims 2
- 239000000741 silica gel Substances 0.000 claims 2
- 229910002027 silica gel Inorganic materials 0.000 claims 2
- 238000005160 1H NMR spectroscopy Methods 0.000 claims 1
- 229920002307 Dextran Polymers 0.000 claims 1
- 239000004793 Polystyrene Substances 0.000 claims 1
- CSCPPACGZOOCGX-WFGJKAKNSA-N acetone d6 Chemical compound [2H]C([2H])([2H])C(=O)C([2H])([2H])[2H] CSCPPACGZOOCGX-WFGJKAKNSA-N 0.000 claims 1
- 238000010564 aerobic fermentation Methods 0.000 claims 1
- -1 alkanoyl ester Chemical class 0.000 claims 1
- VZTDIZULWFCMLS-UHFFFAOYSA-N ammonium formate Chemical compound [NH4+].[O-]C=O VZTDIZULWFCMLS-UHFFFAOYSA-N 0.000 claims 1
- 238000004587 chromatography analysis Methods 0.000 claims 1
- 238000004440 column chromatography Methods 0.000 claims 1
- 239000003480 eluent Substances 0.000 claims 1
- 238000000605 extraction Methods 0.000 claims 1
- 229930195733 hydrocarbon Natural products 0.000 claims 1
- 150000002430 hydrocarbons Chemical class 0.000 claims 1
- 238000007654 immersion Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 claims 1
- 230000014759 maintenance of location Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000001819 mass spectrum Methods 0.000 claims 1
- FPLYNRPOIZEADP-UHFFFAOYSA-N octylsilane Chemical compound CCCCCCCC[SiH3] FPLYNRPOIZEADP-UHFFFAOYSA-N 0.000 claims 1
- 229920002223 polystyrene Polymers 0.000 claims 1
- 239000002244 precipitate Substances 0.000 claims 1
- 239000002904 solvent Substances 0.000 claims 1
- 239000000725 suspension Substances 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/06—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using actinomycetales
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Virology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Compounds Of Unknown Constitution (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 본 발명의 항생물질의 자외선흡수 스펙트럼임, 제2도는 본 발명의 항생물질의 적외선흡수 스펙트럼임, 제3도는 본 발명의 항생물질의 NMR스펙트럼임.
Claims (10)
- A) 다음의 흡수최대치를 나타내는 자외선 흡수스펙트럼:B) 다음의 흡수최대치를 나타내는 적외선 흡수스펙트럼(cm-1):3700-3080, 2980-2840(nujol); 1645; 1560; 1455(nujol); 1375(nujol); 1305; 1240; 1215; 1145; 1090; 1060; 1035; 990; 945; 910; 890; 850; 810; 720(nujol).C) 내부기준치(0.00ppm)로서 TMS를 사용한 헥사듀테로아세톤중에서 270MHz1H-NMR에서 다음 신호군(ppm)으로 기록된 NMR 스펙트럼(δ=pp); 0.77δ(3H); 0.83δ(3H); 1.21-1.24δ(3H); 1.2-2.7δ(5H); 1.67δ(s,3H); 1.73δ(3H); 2.01δ(s,3H); 3.20δ(s, 3H); 3.3-5.6δ(11H); 5.4-6.7δ(13H); 6.04δ(1H); 7.40δ(1H); 7.68δ(1H),10.53δ(1H)(s=단일선)D) HP 5985B 장치를 사용하고, 음이온형으로 작동되고, 다음 조건하에서 LC를 조작하는 직접 액체유입 LC-MS로 얻어진 질량스펙트럼.칼럼 : Brownlee Lab. RP 8 10㎛, 25㎝(옥틸실란 기능처리된 실리카겔)유속 : 1ml/분용출액 : 아세토니트릴 : 0.1M포름산암모늄=60:40 이것은 각각 4.48, 5.03, 5.65 및 7.25분의 체류시간의 4개의 주요피크를 나타내고, 이것은 항생물질 SB 22484인자들 1,2,3 및 4로 각각 임의로 명명함.E) 상기한 항생물질 인자들, 즉 항생물질 SB 22484인자들 1,2,3 및 4에 대한 다음과 같은 주요분할피크:인자 1:732, 734, 716, 684, 538, 598, 566, 500 인자 2 : 766, 748, 730, 698, 652, 612, 580 인자 3: 752, 734, 716, 684, 638, 598, 566, 500 인자 4 : 766, 748, 730, 698, 652, 612, 580, 500F) 실관화실리카겔 60F254Merck 플레이트를 사용하여 각종 크로마토그라피 시스템에서 다음의 Rf값 :가시 : 254nm의 자외선유리산형에서 위와 같은 특성을 갖는 항생물질 SB 22484 또는 약리적으로 허용되는 그의 염.
- 4개의 주요성분, 즉 항생물질 SB 22484인자, 1,2,3 및 4를 어떠한 비율로 본질적으로 함유하는 혼합물로서의 항생물질 SB 22484.
- 스트렙토마이세스종 NRRL 15496 또는 항생물질 SB 22484를 생성하는 상기 균주의 변이체 또는 돌연변이체를 동화될 수 있는 탄소, 질소 및 무기염원의 존재하에 침지 호기조건하에 배양시키고, 발효액에서 상기 항생물질을 회수 및 단리시키고, 필요한 염으로 이것을 전위시키는 항생물질 SB 22484 또는 약리학적으로 허용되는 그의 염의 제조방법.
- 제3에 있어서, 생성균주의 배양을 20°-40℃에서 행하는 방법.
- 제3항에 있어서, 항생물질의 회수를 물과 혼합되지 않는 저급알킬알카노일산에스테르, 저급폴리할로겐화탄화수소 및 고급알칸올에서 선별된 용매로 여과시킨 발효액을 추출시켜서 행하는 방법.
- 제2항에 있어서, 항생물질의 단리를 유도조절된 기공가교시킨 덱스트란 또는 비기능폴리스티렌을 사용하여 입체추출 칼럼크로마토그라피로 행하는 방법.
- 제6항에 있어서, 크로마토그라피칼럼을 아세톤, 메탄올, 에탄올, n-프로판올, 이소프로판올 또는 테트라히드푸란의 2성분계 혼합물 10 내지 90%로 용출시키는 방법.
- 제3항에 있어서, 항생물질의 회수를 발효액의 pH를 약 7.5로 조정하고, 이것을 물과 혼화되지 않는 저급알킬알카노일에스테르로 추출시켜서 pH를 약 9.0으로 조절시키고, 여과시켜 회수하고, 형성되는 침전을 물에 용해시키고, 이 현탄액의 pH를 약 4로 조절시키고, 이것은 물과 혼화되지 않는 저급알카노일에스테르로 추출시킨 다음, 이 추출액의 pH를 약 9로 조절시켜서 다시 침전시키는 방법.
- 스트렙토마이세스종 NRRL 15496 또는 그 변이체.
- 동화될 수 있는 탄소, 질소 및 무기염원의 존재하에 호기발효조건이 될 때에 항생물질 BS 22484를 생성할 수 있는 스트렙토마이세스종 NRRL 15496 또는 이것의 변이체 또는 돌연변이체의 생물학적 순수배양체.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB23382 | 1983-08-31 | ||
| GB8323382 | 1983-08-31 | ||
| GB838323382A GB8323382D0 (en) | 1983-08-31 | 1983-08-31 | Antibiotic sb 22484 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR850001538A true KR850001538A (ko) | 1985-03-30 |
| KR920001373B1 KR920001373B1 (ko) | 1992-02-11 |
Family
ID=10548129
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019840005335A KR920001373B1 (ko) | 1983-08-31 | 1984-08-30 | 항생 물질 sb 22484의 제조 방법 |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US4607012A (ko) |
| EP (1) | EP0139158B1 (ko) |
| JP (1) | JPH067797B2 (ko) |
| KR (1) | KR920001373B1 (ko) |
| AT (1) | ATE40378T1 (ko) |
| AU (1) | AU581621B2 (ko) |
| CA (1) | CA1228313A (ko) |
| DE (1) | DE3476396D1 (ko) |
| DK (1) | DK161150C (ko) |
| ES (1) | ES8602126A1 (ko) |
| FI (1) | FI77691C (ko) |
| GB (1) | GB8323382D0 (ko) |
| GR (1) | GR80076B (ko) |
| HU (1) | HU193696B (ko) |
| IE (1) | IE57903B1 (ko) |
| IL (1) | IL72688A (ko) |
| NO (1) | NO159537C (ko) |
| NZ (1) | NZ209398A (ko) |
| PT (1) | PT79161B (ko) |
| ZA (1) | ZA846451B (ko) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0406745B1 (en) * | 1989-07-04 | 1995-01-25 | GRUPPO LEPETIT S.p.A. | Antibiotic GE 2270 factors A1, A2, A3 and H |
| US5814704A (en) * | 1997-03-04 | 1998-09-29 | Virus Research Institute, Inc. | Recovery of polyphosphazene polyacids or acids salts thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4313935A (en) * | 1979-03-05 | 1982-02-02 | Fujisawa Pharmaceutical Co., Ltd. | Antibiotic FR-900129 substance, a process for the preparation thereof and pharmaceutical compositions containing the same |
-
1983
- 1983-08-31 GB GB838323382A patent/GB8323382D0/en active Pending
-
1984
- 1984-08-10 GR GR80076A patent/GR80076B/el unknown
- 1984-08-15 IL IL72688A patent/IL72688A/xx unknown
- 1984-08-17 AU AU32030/84A patent/AU581621B2/en not_active Ceased
- 1984-08-20 ZA ZA846451A patent/ZA846451B/xx unknown
- 1984-08-22 DE DE8484109984T patent/DE3476396D1/de not_active Expired
- 1984-08-22 AT AT84109984T patent/ATE40378T1/de active
- 1984-08-22 EP EP84109984A patent/EP0139158B1/en not_active Expired
- 1984-08-28 DK DK411584A patent/DK161150C/da not_active IP Right Cessation
- 1984-08-28 CA CA000461926A patent/CA1228313A/en not_active Expired
- 1984-08-29 JP JP59178572A patent/JPH067797B2/ja not_active Expired - Lifetime
- 1984-08-30 IE IE2219/84A patent/IE57903B1/en not_active IP Right Cessation
- 1984-08-30 NZ NZ209398A patent/NZ209398A/en unknown
- 1984-08-30 PT PT79161A patent/PT79161B/pt not_active IP Right Cessation
- 1984-08-30 NO NO843446A patent/NO159537C/no unknown
- 1984-08-30 KR KR1019840005335A patent/KR920001373B1/ko not_active IP Right Cessation
- 1984-08-30 US US06/646,178 patent/US4607012A/en not_active Expired - Lifetime
- 1984-08-30 HU HU843259A patent/HU193696B/hu not_active IP Right Cessation
- 1984-08-30 ES ES535512A patent/ES8602126A1/es not_active Expired
- 1984-08-30 FI FI843418A patent/FI77691C/fi not_active IP Right Cessation
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