KR840002771A - 인슐린, 인슐린 유도체 및 불순물의 혼합물을 분리하는 방법 - Google Patents

인슐린, 인슐린 유도체 및 불순물의 혼합물을 분리하는 방법 Download PDF

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Publication number
KR840002771A
KR840002771A KR1019820005395A KR820005395A KR840002771A KR 840002771 A KR840002771 A KR 840002771A KR 1019820005395 A KR1019820005395 A KR 1019820005395A KR 820005395 A KR820005395 A KR 820005395A KR 840002771 A KR840002771 A KR 840002771A
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KR
South Korea
Prior art keywords
insulin
mixture
volumes
impurities
separate
Prior art date
Application number
KR1019820005395A
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English (en)
Other versions
KR880002607B1 (ko
Inventor
오버마이어 라이너 (외 2)
Original Assignee
하인리히 베커,베른하르트 베크
훽스트 아크티엔 게젤샤프트
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Application filed by 하인리히 베커,베른하르트 베크, 훽스트 아크티엔 게젤샤프트 filed Critical 하인리히 베커,베른하르트 베크
Publication of KR840002771A publication Critical patent/KR840002771A/ko
Application granted granted Critical
Publication of KR880002607B1 publication Critical patent/KR880002607B1/ko

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C07K14/625—Extraction from natural sources

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Diabetes (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

내용 없음.

Description

인슐린, 인슐린 유도체 및 불순물의 혼합물을 분리하는 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (2)

  1. 인슐린, 인슐린 유도체 및, 경우에 따라서, 불순물의 혼합물을 실리카겔로 충전된 컬럼상에서 크로마토그라피함을 특징으로 하는, 이 혼합물의 분리 방법.
  2. 제1항에 있어서, 크로마토그라피를 클로로포름 1,500 내지 2,100 용량부, 메탄올 1,000 내지 1,500 용량부, 물 350 내지 450 용량부, 트리에틸아민 35 내지 용량부 및 포름산 0 내지 15용량부의 용출제 혼합물 중에서 진행시키는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR8205395A 1981-12-03 1982-12-03 인슐린, 인슐린 유도체 및 불순물의 혼합물을 분리하는 방법 KR880002607B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19813147842 DE3147842A1 (de) 1981-12-03 1981-12-03 "verfahren zur trennung von gemischen aus insulin, insulinderivaten und gegebenenfalls verunreinigungen"
DE3147842.5 1981-12-03

Publications (2)

Publication Number Publication Date
KR840002771A true KR840002771A (ko) 1984-07-16
KR880002607B1 KR880002607B1 (ko) 1988-12-04

Family

ID=6147790

Family Applications (1)

Application Number Title Priority Date Filing Date
KR8205395A KR880002607B1 (ko) 1981-12-03 1982-12-03 인슐린, 인슐린 유도체 및 불순물의 혼합물을 분리하는 방법

Country Status (15)

Country Link
US (1) US4677192A (ko)
EP (1) EP0082359B1 (ko)
JP (1) JPS58103323A (ko)
KR (1) KR880002607B1 (ko)
AR (1) AR230430A1 (ko)
AT (1) ATE18057T1 (ko)
AU (1) AU563350B2 (ko)
CA (1) CA1245633A (ko)
DE (2) DE3147842A1 (ko)
DK (1) DK162104C (ko)
ES (1) ES8607343A1 (ko)
FI (1) FI75349C (ko)
GR (1) GR77764B (ko)
IL (1) IL67378A (ko)
ZA (1) ZA828864B (ko)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3511269A1 (de) * 1985-04-12 1986-10-09 Veb Berlin-Chemie, Ddr 1199 Berlin Verfahren zur reinigung von insulin
DE4028120C2 (de) * 1990-09-05 1996-09-19 Hoechst Ag Verfahren zur Reinigung von Insulin und/oder Insulinderivaten
US5278284A (en) * 1992-05-14 1994-01-11 Miller Brewing Company Protein purification method
US5451660A (en) * 1993-12-13 1995-09-19 Genentech, Inc. Method for purifying polypeptides
DE19652713C2 (de) * 1996-12-18 2001-11-22 Aventis Pharma Gmbh Verfahren zur Reinigung von Insulin und Insulinderivaten durch Chromatographie an stark saurem Kationenaustauscher
WO2016014325A1 (en) 2014-07-21 2016-01-28 Merck Sharp & Dohme Corp. Chromatography process for purification of inlsulin and insulin analogs

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS532492A (en) * 1976-06-26 1978-01-11 Shimizu Seiyaku Kk Process for preparing pure insuline
JPS5542185A (en) * 1978-09-22 1980-03-25 Kawasaki Heavy Ind Ltd Detecting device for arc welding or the like
JPS6016673B2 (ja) * 1978-12-25 1985-04-26 川崎重工業株式会社 サ−ボ系における被検体認識装置

Also Published As

Publication number Publication date
KR880002607B1 (ko) 1988-12-04
DE3269252D1 (en) 1986-03-27
US4677192A (en) 1987-06-30
ATE18057T1 (de) 1986-03-15
JPS58103323A (ja) 1983-06-20
DK162104B (da) 1991-09-16
DK536282A (da) 1983-06-04
AU563350B2 (en) 1987-07-09
FI824135L (fi) 1983-06-04
GR77764B (ko) 1984-09-25
IL67378A0 (en) 1983-05-15
FI75349B (fi) 1988-02-29
EP0082359B1 (de) 1986-02-19
DK162104C (da) 1992-02-17
ES8607343A1 (es) 1986-05-16
AU9110282A (en) 1983-06-09
DE3147842A1 (de) 1983-06-23
AR230430A1 (es) 1984-04-30
FI75349C (fi) 1988-06-09
EP0082359A1 (de) 1983-06-29
CA1245633A (en) 1988-11-29
ZA828864B (en) 1984-05-30
JPH0354678B2 (ko) 1991-08-20
IL67378A (en) 1985-12-31
ES517844A0 (es) 1986-05-16
FI824135A0 (fi) 1982-12-01

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