KR840002771A - 인슐린, 인슐린 유도체 및 불순물의 혼합물을 분리하는 방법 - Google Patents
인슐린, 인슐린 유도체 및 불순물의 혼합물을 분리하는 방법 Download PDFInfo
- Publication number
- KR840002771A KR840002771A KR1019820005395A KR820005395A KR840002771A KR 840002771 A KR840002771 A KR 840002771A KR 1019820005395 A KR1019820005395 A KR 1019820005395A KR 820005395 A KR820005395 A KR 820005395A KR 840002771 A KR840002771 A KR 840002771A
- Authority
- KR
- South Korea
- Prior art keywords
- insulin
- mixture
- volumes
- impurities
- separate
- Prior art date
Links
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 title claims 6
- 239000000203 mixture Substances 0.000 title claims 4
- 102000004877 Insulin Human genes 0.000 title claims 2
- 108090001061 Insulin Proteins 0.000 title claims 2
- 239000012535 impurity Substances 0.000 title claims 2
- 229940125396 insulin Drugs 0.000 title claims 2
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims 3
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 claims 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 1
- 238000004587 chromatography analysis Methods 0.000 claims 1
- 239000003480 eluent Substances 0.000 claims 1
- 235000019253 formic acid Nutrition 0.000 claims 1
- 239000000741 silica gel Substances 0.000 claims 1
- 229910002027 silica gel Inorganic materials 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C07K14/625—Extraction from natural sources
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Diabetes (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
내용 없음.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (2)
- 인슐린, 인슐린 유도체 및, 경우에 따라서, 불순물의 혼합물을 실리카겔로 충전된 컬럼상에서 크로마토그라피함을 특징으로 하는, 이 혼합물의 분리 방법.
- 제1항에 있어서, 크로마토그라피를 클로로포름 1,500 내지 2,100 용량부, 메탄올 1,000 내지 1,500 용량부, 물 350 내지 450 용량부, 트리에틸아민 35 내지 용량부 및 포름산 0 내지 15용량부의 용출제 혼합물 중에서 진행시키는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE3147842.5 | 1981-12-03 | ||
DE19813147842 DE3147842A1 (de) | 1981-12-03 | 1981-12-03 | "verfahren zur trennung von gemischen aus insulin, insulinderivaten und gegebenenfalls verunreinigungen" |
Publications (2)
Publication Number | Publication Date |
---|---|
KR840002771A true KR840002771A (ko) | 1984-07-16 |
KR880002607B1 KR880002607B1 (ko) | 1988-12-04 |
Family
ID=6147790
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR8205395A KR880002607B1 (ko) | 1981-12-03 | 1982-12-03 | 인슐린, 인슐린 유도체 및 불순물의 혼합물을 분리하는 방법 |
Country Status (15)
Country | Link |
---|---|
US (1) | US4677192A (ko) |
EP (1) | EP0082359B1 (ko) |
JP (1) | JPS58103323A (ko) |
KR (1) | KR880002607B1 (ko) |
AR (1) | AR230430A1 (ko) |
AT (1) | ATE18057T1 (ko) |
AU (1) | AU563350B2 (ko) |
CA (1) | CA1245633A (ko) |
DE (2) | DE3147842A1 (ko) |
DK (1) | DK162104C (ko) |
ES (1) | ES8607343A1 (ko) |
FI (1) | FI75349C (ko) |
GR (1) | GR77764B (ko) |
IL (1) | IL67378A (ko) |
ZA (1) | ZA828864B (ko) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3511269A1 (de) * | 1985-04-12 | 1986-10-09 | Veb Berlin-Chemie, Ddr 1199 Berlin | Verfahren zur reinigung von insulin |
DE4028120C2 (de) * | 1990-09-05 | 1996-09-19 | Hoechst Ag | Verfahren zur Reinigung von Insulin und/oder Insulinderivaten |
US5278284A (en) * | 1992-05-14 | 1994-01-11 | Miller Brewing Company | Protein purification method |
US5451660A (en) * | 1993-12-13 | 1995-09-19 | Genentech, Inc. | Method for purifying polypeptides |
DE19652713C2 (de) * | 1996-12-18 | 2001-11-22 | Aventis Pharma Gmbh | Verfahren zur Reinigung von Insulin und Insulinderivaten durch Chromatographie an stark saurem Kationenaustauscher |
EP3171888B1 (en) | 2014-07-21 | 2022-12-28 | Merck Sharp & Dohme LLC | Chromatography process for purification of inlsulin and insulin analogs |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS532492A (en) * | 1976-06-26 | 1978-01-11 | Shimizu Seiyaku Kk | Process for preparing pure insuline |
JPS5542185A (en) * | 1978-09-22 | 1980-03-25 | Kawasaki Heavy Ind Ltd | Detecting device for arc welding or the like |
JPS6016673B2 (ja) * | 1978-12-25 | 1985-04-26 | 川崎重工業株式会社 | サ−ボ系における被検体認識装置 |
-
1981
- 1981-12-03 DE DE19813147842 patent/DE3147842A1/de not_active Withdrawn
-
1982
- 1982-11-30 AT AT82111061T patent/ATE18057T1/de not_active IP Right Cessation
- 1982-11-30 EP EP82111061A patent/EP0082359B1/de not_active Expired
- 1982-11-30 DE DE8282111061T patent/DE3269252D1/de not_active Expired
- 1982-12-01 FI FI824135A patent/FI75349C/fi not_active IP Right Cessation
- 1982-12-01 AR AR291468A patent/AR230430A1/es active
- 1982-12-01 ES ES517844A patent/ES8607343A1/es not_active Expired
- 1982-12-01 IL IL67378A patent/IL67378A/xx not_active IP Right Cessation
- 1982-12-01 GR GR69942A patent/GR77764B/el unknown
- 1982-12-02 JP JP57210699A patent/JPS58103323A/ja active Granted
- 1982-12-02 ZA ZA828864A patent/ZA828864B/xx unknown
- 1982-12-02 CA CA000416875A patent/CA1245633A/en not_active Expired
- 1982-12-02 DK DK536282A patent/DK162104C/da not_active IP Right Cessation
- 1982-12-02 AU AU91102/82A patent/AU563350B2/en not_active Ceased
- 1982-12-03 KR KR8205395A patent/KR880002607B1/ko active
-
1985
- 1985-12-04 US US06/804,325 patent/US4677192A/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
GR77764B (ko) | 1984-09-25 |
IL67378A (en) | 1985-12-31 |
DE3269252D1 (en) | 1986-03-27 |
US4677192A (en) | 1987-06-30 |
FI824135L (fi) | 1983-06-04 |
FI75349B (fi) | 1988-02-29 |
DK162104C (da) | 1992-02-17 |
DE3147842A1 (de) | 1983-06-23 |
ES8607343A1 (es) | 1986-05-16 |
AR230430A1 (es) | 1984-04-30 |
IL67378A0 (en) | 1983-05-15 |
CA1245633A (en) | 1988-11-29 |
KR880002607B1 (ko) | 1988-12-04 |
ZA828864B (en) | 1984-05-30 |
ATE18057T1 (de) | 1986-03-15 |
DK536282A (da) | 1983-06-04 |
FI75349C (fi) | 1988-06-09 |
JPS58103323A (ja) | 1983-06-20 |
ES517844A0 (es) | 1986-05-16 |
JPH0354678B2 (ko) | 1991-08-20 |
EP0082359B1 (de) | 1986-02-19 |
AU563350B2 (en) | 1987-07-09 |
FI824135A0 (fi) | 1982-12-01 |
AU9110282A (en) | 1983-06-09 |
EP0082359A1 (de) | 1983-06-29 |
DK162104B (da) | 1991-09-16 |
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