ES8607343A1 - Procedimiento para la separacion de mezclas a base de insulina, derivados de insulina y, eventualmente, impurezas. - Google Patents

Procedimiento para la separacion de mezclas a base de insulina, derivados de insulina y, eventualmente, impurezas.

Info

Publication number
ES8607343A1
ES8607343A1 ES517844A ES517844A ES8607343A1 ES 8607343 A1 ES8607343 A1 ES 8607343A1 ES 517844 A ES517844 A ES 517844A ES 517844 A ES517844 A ES 517844A ES 8607343 A1 ES8607343 A1 ES 8607343A1
Authority
ES
Spain
Prior art keywords
insulin
derivatives
mixtures
impurities
separation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES517844A
Other languages
English (en)
Other versions
ES517844A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of ES8607343A1 publication Critical patent/ES8607343A1/es
Publication of ES517844A0 publication Critical patent/ES517844A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C07K14/625—Extraction from natural sources

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Diabetes (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

MEJORA EN UN PROCEDIMIENTO PARA LA SEPARACION DE MEZCLAS A BASE DE INSULINA, DERIVADOS DE INSULINA Y, EVENTUALMENTE, IMPUREZAS. CONSISTE EN: A) CARGAR LA MEZCLA EN UNA COLUMNA RELLENA DE CUALQUIER GEL DE SILICE ADQUIRIBLE EN EL COMERCIO; B) ELUIR LA COLUMNA RELLENA DE GEL DE SILICE CON UNA MEZCLA DE ELUYENTES A BASE DE 1.500-2.100 PARTES EN VOLUMEN DE CLOROFORMO, 1.000-1.500 PARTES EN VOLUMEN DE METANOL, 350-450 PARTES EN VOLUMEN DE AGUA, 35-55 PARTES EN VOLUMEN DE TRIETILAMINA Y 0-15 PARTES EN VOLUMEN DE ACIDO FORMICO; C) RECOGER Y TRATAR LAS FRACCIONES INDIVIDUALES. SE UTILIZA EN EL TRATAMIENTO DE LA DIABETES MELLITUS HUMANA.
ES517844A 1981-12-03 1982-12-01 Procedimiento para la separacion de mezclas a base de insulina, derivados de insulina y, eventualmente, impurezas. Expired ES8607343A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19813147842 DE3147842A1 (de) 1981-12-03 1981-12-03 "verfahren zur trennung von gemischen aus insulin, insulinderivaten und gegebenenfalls verunreinigungen"

Publications (2)

Publication Number Publication Date
ES8607343A1 true ES8607343A1 (es) 1986-05-16
ES517844A0 ES517844A0 (es) 1986-05-16

Family

ID=6147790

Family Applications (1)

Application Number Title Priority Date Filing Date
ES517844A Expired ES8607343A1 (es) 1981-12-03 1982-12-01 Procedimiento para la separacion de mezclas a base de insulina, derivados de insulina y, eventualmente, impurezas.

Country Status (15)

Country Link
US (1) US4677192A (es)
EP (1) EP0082359B1 (es)
JP (1) JPS58103323A (es)
KR (1) KR880002607B1 (es)
AR (1) AR230430A1 (es)
AT (1) ATE18057T1 (es)
AU (1) AU563350B2 (es)
CA (1) CA1245633A (es)
DE (2) DE3147842A1 (es)
DK (1) DK162104C (es)
ES (1) ES8607343A1 (es)
FI (1) FI75349C (es)
GR (1) GR77764B (es)
IL (1) IL67378A (es)
ZA (1) ZA828864B (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3511269A1 (de) * 1985-04-12 1986-10-09 Veb Berlin-Chemie, Ddr 1199 Berlin Verfahren zur reinigung von insulin
DE4028120C2 (de) * 1990-09-05 1996-09-19 Hoechst Ag Verfahren zur Reinigung von Insulin und/oder Insulinderivaten
US5278284A (en) * 1992-05-14 1994-01-11 Miller Brewing Company Protein purification method
US5451660A (en) * 1993-12-13 1995-09-19 Genentech, Inc. Method for purifying polypeptides
DE19652713C2 (de) * 1996-12-18 2001-11-22 Aventis Pharma Gmbh Verfahren zur Reinigung von Insulin und Insulinderivaten durch Chromatographie an stark saurem Kationenaustauscher
WO2016014325A1 (en) 2014-07-21 2016-01-28 Merck Sharp & Dohme Corp. Chromatography process for purification of inlsulin and insulin analogs

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS532492A (en) * 1976-06-26 1978-01-11 Shimizu Seiyaku Kk Process for preparing pure insuline
JPS5542185A (en) * 1978-09-22 1980-03-25 Kawasaki Heavy Ind Ltd Detecting device for arc welding or the like
JPS6016673B2 (ja) * 1978-12-25 1985-04-26 川崎重工業株式会社 サ−ボ系における被検体認識装置

Also Published As

Publication number Publication date
JPS58103323A (ja) 1983-06-20
IL67378A0 (en) 1983-05-15
AU563350B2 (en) 1987-07-09
FI824135L (fi) 1983-06-04
EP0082359B1 (de) 1986-02-19
KR880002607B1 (ko) 1988-12-04
AR230430A1 (es) 1984-04-30
DE3147842A1 (de) 1983-06-23
DK162104B (da) 1991-09-16
DK536282A (da) 1983-06-04
ZA828864B (en) 1984-05-30
EP0082359A1 (de) 1983-06-29
FI75349B (fi) 1988-02-29
ATE18057T1 (de) 1986-03-15
AU9110282A (en) 1983-06-09
US4677192A (en) 1987-06-30
GR77764B (es) 1984-09-25
KR840002771A (ko) 1984-07-16
DE3269252D1 (en) 1986-03-27
DK162104C (da) 1992-02-17
JPH0354678B2 (es) 1991-08-20
ES517844A0 (es) 1986-05-16
IL67378A (en) 1985-12-31
FI75349C (fi) 1988-06-09
CA1245633A (en) 1988-11-29
FI824135A0 (fi) 1982-12-01

Similar Documents

Publication Publication Date Title
IL63662A (en) Chromatography column and method for effecting molecular separation
AU6884681A (en) Process and apparatus for the separation of gaseous mixtures
DE3564378D1 (en) Pressure swing process for the adsorptive separation of gas mixtures
DE3268025D1 (en) Process for the adsorptive separation of a gas mixture
ZA829345B (en) Gas separation apparatus
AU3836685A (en) Method and apparatus for separating impurities from low conductivity liquids
AU1938283A (en) Integrated process for the solvent refining of coal
DE3261834D1 (en) Process for the separation of gas mixtures by pressure-swing adsorption
ES8607343A1 (es) Procedimiento para la separacion de mezclas a base de insulina, derivados de insulina y, eventualmente, impurezas.
DE3363964D1 (en) Device operating according to the principle of gas chromatography for separating hydrogen isotopes
GB2109266B (en) Pressure swing process for the separation of gas mixtures by adsorption
YU41969B (en) Process for the separation of fructose from glucose in a mixture comprising the same
AU1957483A (en) Apparatus for separating ore
YU143185A (en) Process for separation of small parts from gasses
MY8500855A (en) Process for the separation of elements by chromatography
ZA817997B (en) Method for the separation of valuable substances from plant matter
GB2108012B (en) Process for the thick-medium dynamic separation of mixtures of materials
EP0062337A3 (en) Process for the recovery of valuable material
AU552243B2 (en) Gas/liquid separator
EP0075817A3 (en) Process for the separation of liquid mixtures by distillation
JPS57147435A (en) Method of separating gas or liquid
DE3571158D1 (en) Apparatus for the separation of plasma from the other constituents of blood
JPS52117696A (en) Separating apparatus for chromatography
AU550033B2 (en) Apparatus for injecting samples into gas chromatograph
YU91981A (en) Process for the isolation of (+)-usnic acod from usnea barbata l.

Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970212