KR840001836A - 생리활성 물질의 제법 - Google Patents

생리활성 물질의 제법 Download PDF

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Publication number
KR840001836A
KR840001836A KR1019820004509A KR820004509A KR840001836A KR 840001836 A KR840001836 A KR 840001836A KR 1019820004509 A KR1019820004509 A KR 1019820004509A KR 820004509 A KR820004509 A KR 820004509A KR 840001836 A KR840001836 A KR 840001836A
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South Korea
Prior art keywords
cells
preparation
bioactive substance
axis represents
cancer cells
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KR1019820004509A
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English (en)
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히로시 애노모또 (외 3)
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모리시다 히로시
닛본 신야구 가부시기 가이샤
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Publication of KR840001836A publication Critical patent/KR840001836A/ko

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/91—Cell lines ; Processes using cell lines

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

내용 없음

Description

생리활성 물질의 제법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 실시예 1에서 얻은 본 발명물질을 (a)는 정상시험쥐 태아세포(흑점)와 L-929의 암세포(백점)에, (b)는 정상 시험쥐 태아세포(흑점)와 HeLa-S3 의 암세포(백점)에 각각 시험관 안에서 작용시킨 경우의 세포장해를 나타낸다. 종축은 색소주입량(OD치)를 횡측은 농도를 표시한다. 실선은 혈청첨가배지를 사용한 경우를, 점설은 무혈청배지를 사용한 경우를 표시한다. 이것으로 본 발명물질이 암세포에만 특이적으로 작용하고, 또 종특이성(種特異性)이 없음을 알 수 있다. 제2도는 표준 단백질의 분자량과 SDS-PAGE에 있어서의 이동도와의 관계를 도시한다. 종축은 분자량을 횡축은 이동도를 나타낸다. a는 우혈청 알브민(BSA)을, b는 난백알브민(OVA)를 C는 기모트리프시노오겐을 표시한다. 흑점이 실시예 1에서 얻은 본 발명 물질의 위치이다. 이것에 의하여 본 발명 물질의 분자량을 알 수 있다. 제3도는 실시예 1에서 얻은 본 발명 물질의 Sephacryl-S 200에 의한 겔 여과 패턴이다. 종축은 세포의 사망율을, 횡축은 프랙션의 번호(1ml/tube)를 나타낸다.

Claims (1)

  1. 동물유래세포 또는 그들의 융합 세포를 배양하고 그 배양액을 정제하는 것을 특징으로 하는 암세포에만 특이적으로 작용하고 정상세포에는 작용하지 않는 항종양활성을 가지는 생리활성물질의 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019820004509A 1982-02-13 1982-10-07 생리활성 물질의 제법 KR840001836A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP57021562A JPS58138383A (ja) 1982-02-13 1982-02-13 生理活性物質の製法
JP21562 1982-02-13

Publications (1)

Publication Number Publication Date
KR840001836A true KR840001836A (ko) 1984-06-07

Family

ID=12058451

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019820004509A KR840001836A (ko) 1982-02-13 1982-10-07 생리활성 물질의 제법

Country Status (5)

Country Link
EP (1) EP0087087A3 (ko)
JP (1) JPS58138383A (ko)
KR (1) KR840001836A (ko)
CA (1) CA1201079A (ko)
ES (1) ES519737A0 (ko)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5933223A (ja) * 1982-08-20 1984-02-23 Koken Kk 人の悪性腫瘍細胞増殖抑制剤
JPS59162889A (ja) * 1983-03-07 1984-09-13 Koken Kk 人の悪性腫瘍細胞の増殖抑制剤の製造方法
JPS59204122A (ja) * 1983-05-06 1984-11-19 Koken Kk 人の悪性腫瘍細胞増殖抑制剤の製造方法
JPS6028930A (ja) * 1983-07-28 1985-02-14 Koken Kk 人の癌由来細胞の増殖抑制剤の精製方法
JPH088873B2 (ja) * 1983-07-28 1996-01-31 大日本製薬株式会社 ヒト癌壊死因子の製造法
JPS6028931A (ja) * 1983-07-28 1985-02-14 Koken Kk ヒト腎細胞癌由来樹立株細胞の増殖抑制剤の濃縮方法
JPS6043386A (ja) * 1983-08-20 1985-03-07 Koken Kk ヒト腎細胞癌由来樹立株細胞の増殖抑制剤の製造方法
US4959457A (en) * 1984-05-31 1990-09-25 Genentech, Inc. Anti-lymphotoxin
US5683688A (en) 1984-05-31 1997-11-04 Genentech, Inc. Unglycosylated recombinant human lymphotoxin polypeptides and compositions
US5672347A (en) * 1984-07-05 1997-09-30 Genentech, Inc. Tumor necrosis factor antagonists and their use
US6686455B1 (en) 1984-07-05 2004-02-03 Genentech, Inc. Tumor necrosis factor
JPS6127923A (ja) * 1984-07-17 1986-02-07 Denichi Mizuno ヒト腫瘍壊死因子の製造法
IL73883A (en) † 1984-12-20 1990-12-23 Yeda Res & Dev Monoclonal antibodies against tnf-alpha,hybridomas producing them and method for the purification of tnf-alpha
JPS61155330A (ja) * 1984-12-28 1986-07-15 Denichi Mizuno 蛋白質標品
JPS6255087A (ja) * 1985-03-25 1987-03-10 シタス　オンコロジー　コーポレイション 融合蛋白質をコ−ドする組換ｄｎａ配列
GB2245415A (en) * 1990-06-22 1992-01-02 Philips Electronic Associated Infrared detector with a getter
US9416086B2 (en) * 2010-11-12 2016-08-16 Eastman Chemical Company Processes for purification of acid solutions
US9415366B2 (en) * 2012-12-31 2016-08-16 Eastman Chemical Company Systems and methods for processing variable acetyl streams

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3865689A (en) * 1972-11-09 1975-02-11 Hoffmann La Roche Method of producing carcinoembryonic antigens
US4195017A (en) * 1975-02-25 1980-03-25 Samuel Bogoch Malignin, derived from brain tumor cells, complexes and polypeptides thereof
FR2302747B1 (fr) * 1975-03-03 1978-09-22 Inst Nat Sante Rech Med Procede d'obtention, a partir de macrophages de mammiferes, d'un produit therapeutique actif, notamment, a l'egard des cellules tumorales humaines et produit therapeutique ainsi obtenu
DE2600442A1 (de) * 1976-01-08 1977-07-14 Karl Dr Med Theurer Herstellung von biologischen arzneimitteln gegen maligne tumoren und blutkrankheiten aus zell- bzw. gewebekulturen
DE2619715A1 (de) * 1976-05-04 1977-11-24 Max Planck Gesellschaft Tumor-antigen und verfahren zu dessen herstellung
US4059486A (en) * 1976-11-03 1977-11-22 Monsanto Company Cell culture process
US4228236A (en) * 1978-05-15 1980-10-14 Northwestern University Process of producing carcinoembryonic antigen

Also Published As

Publication number Publication date
CA1201079A (en) 1986-02-25
ES8403157A1 (es) 1984-03-01
JPS58138383A (ja) 1983-08-17
EP0087087A3 (en) 1983-10-19
EP0087087A2 (en) 1983-08-31
ES519737A0 (es) 1984-03-01

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