KR20250022761A - 세포의 작출 방법, 헤테로 다량체 단백질의 제조 방법, 바이스페시픽 항체의 제조 방법, 벡터 세트, 포유동물 세포, cho 세포, 및 세포 풀의 작출 방법 - Google Patents

세포의 작출 방법, 헤테로 다량체 단백질의 제조 방법, 바이스페시픽 항체의 제조 방법, 벡터 세트, 포유동물 세포, cho 세포, 및 세포 풀의 작출 방법 Download PDF

Info

Publication number
KR20250022761A
KR20250022761A KR1020257000217A KR20257000217A KR20250022761A KR 20250022761 A KR20250022761 A KR 20250022761A KR 1020257000217 A KR1020257000217 A KR 1020257000217A KR 20257000217 A KR20257000217 A KR 20257000217A KR 20250022761 A KR20250022761 A KR 20250022761A
Authority
KR
South Korea
Prior art keywords
expression
cell
chain
vector
expression vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
KR1020257000217A
Other languages
English (en)
Korean (ko)
Inventor
타츠야 마츠우라
아카리 쿠로다
Original Assignee
후지필름 다이오신쓰 바이오테크놀로지스 유케 리미티드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 후지필름 다이오신쓰 바이오테크놀로지스 유케 리미티드 filed Critical 후지필름 다이오신쓰 바이오테크놀로지스 유케 리미티드
Publication of KR20250022761A publication Critical patent/KR20250022761A/ko
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515—Complete light chain, i.e. VL + CL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/64—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising a combination of variable region and constant region components
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y105/00—Oxidoreductases acting on the CH-NH group of donors (1.5)
    • C12Y105/01—Oxidoreductases acting on the CH-NH group of donors (1.5) with NAD+ or NADP+ as acceptor (1.5.1)
    • C12Y105/01003—Dihydrofolate reductase (1.5.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
    • C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Oncology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020257000217A 2022-06-10 2023-06-09 세포의 작출 방법, 헤테로 다량체 단백질의 제조 방법, 바이스페시픽 항체의 제조 방법, 벡터 세트, 포유동물 세포, cho 세포, 및 세포 풀의 작출 방법 Pending KR20250022761A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JPJP-P-2022-094577 2022-06-10
JP2022094577 2022-06-10
PCT/JP2023/021621 WO2023238949A1 (ja) 2022-06-10 2023-06-09 細胞の作出方法、ヘテロ多量体タンパク質の製造方法、バイスペシフィック抗体の製造方法、ベクターセット、哺乳動物細胞、ｃｈｏ細胞、及び細胞プールの作出方法

Publications (1)

Publication Number Publication Date
KR20250022761A true KR20250022761A (ko) 2025-02-17

Family

ID=89118463

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020257000217A Pending KR20250022761A (ko) 2022-06-10 2023-06-09 세포의 작출 방법, 헤테로 다량체 단백질의 제조 방법, 바이스페시픽 항체의 제조 방법, 벡터 세트, 포유동물 세포, cho 세포, 및 세포 풀의 작출 방법

Country Status (6)

Country Link
US (1) US20250361524A1 (https=)
EP (1) EP4538379A1 (https=)
JP (1) JPWO2023238949A1 (https=)
KR (1) KR20250022761A (https=)
CN (1) CN119654417A (https=)
WO (1) WO2023238949A1 (https=)

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012067176A (ja) 2010-09-22 2012-04-05 Daicel-Evonik Ltd 粉末状封止剤及び封止方法
JP2014177826A (ja) 2013-03-15 2014-09-25 Giken Seisakusho Co Ltd 地質推定方法及び地質推定システム
JP2015503907A (ja) 2011-12-22 2015-02-05 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 真核細胞のための全長抗体提示システムおよびその使用
JP2016047722A (ja) 2015-11-11 2016-04-07 三菱製鋼株式会社 懸架コイルばね及びストラット型懸架装置
JP2017501706A (ja) 2013-12-20 2017-01-19 エフ・ホフマン−ラ・ロシュ・アクチェンゲゼルシャフト 二重特異性ｈｅｒ２抗体及び使用方法
JP2018504113A (ja) 2015-01-08 2018-02-15 蘇州康寧傑瑞生物科技有限公司 共通軽鎖を有する二重特異性抗体又は抗体混合物
JP2019516396A (ja) 2016-03-25 2019-06-20 ビオミューネクス・ファルマシューティカルＢｉｏｍｕｎｅｘ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ Ｃｄ３８及びｐｄ−ｌ１に対する結合分子
JP2019093342A (ja) 2017-11-22 2019-06-20 株式会社ジャパンユニックス ミキサー
JP2019528083A (ja) 2016-06-20 2019-10-10 カイマブ・リミテッド 抗ｐｄ−ｌ１抗体
JP2020532281A (ja) 2017-07-06 2020-11-12 メルス ナムローゼ フェンノートシャップ 二重特異性抗ｐｄ−１抗ｔｉｍ３抗体

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101618767B1 (ko) * 2008-09-02 2016-05-09 한국생명공학연구원 외래단백질을 고효율로 생산하는 리더서열
TWI452136B (zh) 2010-11-17 2014-09-11 中外製藥股份有限公司 A multiple specific antigen-binding molecule that replaces the function of Factor VIII in blood coagulation
US9873891B2 (en) 2013-05-03 2018-01-23 Fujifilm Diosynth Biotechnologies Uk Limited Expression process
MA40764A (fr) 2014-09-26 2017-08-01 Chugai Pharmaceutical Co Ltd Agent thérapeutique induisant une cytotoxicité
SG11201807885PA (en) * 2016-04-20 2018-10-30 Regeneron Pharma Compositions and methods for making antibodies based on use of expression-enhancing loci
CA3081854A1 (en) 2017-11-08 2019-05-16 Kyowa Kirin Co., Ltd. Bispecific antibody which binds to cd40 and epcam
JP7650583B2 (ja) 2020-12-15 2025-03-25 株式会社ディスコ チップの製造方法

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012067176A (ja) 2010-09-22 2012-04-05 Daicel-Evonik Ltd 粉末状封止剤及び封止方法
JP2015503907A (ja) 2011-12-22 2015-02-05 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 真核細胞のための全長抗体提示システムおよびその使用
JP2014177826A (ja) 2013-03-15 2014-09-25 Giken Seisakusho Co Ltd 地質推定方法及び地質推定システム
JP2017501706A (ja) 2013-12-20 2017-01-19 エフ・ホフマン−ラ・ロシュ・アクチェンゲゼルシャフト 二重特異性ｈｅｒ２抗体及び使用方法
JP2018504113A (ja) 2015-01-08 2018-02-15 蘇州康寧傑瑞生物科技有限公司 共通軽鎖を有する二重特異性抗体又は抗体混合物
JP2016047722A (ja) 2015-11-11 2016-04-07 三菱製鋼株式会社 懸架コイルばね及びストラット型懸架装置
JP2019516396A (ja) 2016-03-25 2019-06-20 ビオミューネクス・ファルマシューティカルＢｉｏｍｕｎｅｘ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ Ｃｄ３８及びｐｄ−ｌ１に対する結合分子
JP2019528083A (ja) 2016-06-20 2019-10-10 カイマブ・リミテッド 抗ｐｄ−ｌ１抗体
JP2020532281A (ja) 2017-07-06 2020-11-12 メルス ナムローゼ フェンノートシャップ 二重特異性抗ｐｄ−１抗ｔｉｍ３抗体
JP2019093342A (ja) 2017-11-22 2019-06-20 株式会社ジャパンユニックス ミキサー

Also Published As

Publication number Publication date
JPWO2023238949A1 (https=) 2023-12-14
US20250361524A1 (en) 2025-11-27
EP4538379A1 (en) 2025-04-16
CN119654417A (zh) 2025-03-18
WO2023238949A1 (ja) 2023-12-14

Similar Documents

Publication Publication Date Title
KR102180660B1 (ko) 발현 방법
KR101734585B1 (ko) 정지 코돈 번역초과에 의한 폴리펩티드 이소형의 세포 표면 표시
JP2011518790A (ja) ポリクローナルタンパク質を製造する方法
JP7037535B2 (ja) 産生細胞株エンハンサー
EP3622072A1 (en) Universal self-regulating mammalian cell line platform for the production of biologics
CN102498212B (zh) Cho/cert细胞系
CN114026224A (zh) 具有sirt-1基因敲除的哺乳动物细胞系
JP2021530225A (ja) 内因性タンパク質のレベルを低下させることによって生物学的産物の産生を改善する方法
KR20250022761A (ko) 세포의 작출 방법, 헤테로 다량체 단백질의 제조 방법, 바이스페시픽 항체의 제조 방법, 벡터 세트, 포유동물 세포, cho 세포, 및 세포 풀의 작출 방법
US20260117258A1 (en) Novel Transposase System
JP7826509B2 (ja) 選択マーカーとしての新規グルタミン合成酵素変異体
KR20250134666A (ko) 세포의 제조 방법, 다중 특이성 항체의 제조 방법, 벡터 세트, 포유 동물 세포, cho 세포, 및 세포 풀의 제조 방법
HK40118945A (en) Method for producing cells, method for producing heteromultimeric protein, method for producing bispecific antibody, and method for producing vector set, mammalian cells, cho cells, and cell pool
US20220090060A1 (en) Identification and production of antigen-specific antibodies
US20240076405A1 (en) Expression vectors for immunoglobulins and applications thereof
Hussain et al. Sequence and Configuration of a Novel Bispecific Antibody Format Impacts Its Production Using Chinese Hamster Ovary (CHO) Cells
HK1167429A (en) Cho/cert cell lines

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

E13 Pre-grant limitation requested

Free format text: ST27 STATUS EVENT CODE: A-2-3-E10-E13-LIM-X000 (AS PROVIDED BY THE NATIONAL OFFICE)

E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-NAP-X000 (AS PROVIDED BY THE NATIONAL OFFICE)

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000