KR20240061487A - Plant-derived preservative composition having antioxidant, whitening and wrinkle improvement and soothing effects, and cosmetic composition comprising the same - Google Patents

Abstract

The present invention relates to a plant-derived preservative composition having antioxidant, whitening, wrinkle-improving and soothing effects, and to a cosmetic composition containing the same, and more specifically, to a plant-derived preservative composition having antioxidant, whitening, wrinkle-improving and soothing effects, and more specifically, to a plant-derived preservative composition, including caprylhydroxamic acid derived from coconut, 1 derived from sugarcane, Contains 3-butylene glycol (1,3-Butylene glycol), caprylyl glycol derived from coconut palm, grape skin extract, lemon extract, lavender extract, Morus bark extract, ivy leaf extract and citrus extract. The mixed composition is a 100% plant-derived preservative that has the effect of suppressing various bacteria, yeast, and mold and has anti-oxidation, whitening, wrinkle improvement, soothing, and antibacterial effects in addition to its anti-oxidation, whitening, anti-wrinkle, soothing, and antibacterial effects, making it a multi-functional cosmetic preservative material that can help the skin. can be used

Description

Plant-derived preservative composition having antioxidant, whitening, wrinkle-improving and soothing effects, and cosmetic composition containing the same

The present invention relates to a plant-derived preservative composition having antioxidant, whitening, wrinkle-improving and soothing effects, and a cosmetic composition containing the same.

Due to numerous accidents caused by inappropriate chemical ingredients in household products, consumers' aversion to chemicals is accelerating, and they are exposed to risks such as fatal accidents caused by humidifier disinfectants, sanitary napkins from large companies containing carcinogens, and eggs containing pesticides. Consumer demand for chemical-free products is increasing. Accordingly, we are trying to develop a complex functional material that has various efficacy effects in addition to the natural preservative effect using a 100% plant-derived preservative using raw materials made of natural extracts that are harmless to the human body and stable.

Preservatives are drugs that prevent the decay of substances, that is, they prevent animal and vegetable organic matter from decaying due to the action of microorganisms, and preservatives are drugs added to preserve them for the purpose of preservation. In general, preservatives are added to foods, cosmetics, and medicines to prevent them from deteriorating and maintain their purity during use. Therefore, it is an essential condition that it be harmless to the human body, and its addition must not damage its quality. In pharmaceuticals, sodium salt of benzoic acid is added to eye drops, homemade preparations, injections, etc. In the case of food, it is also called a food preservative, and its use is regulated by law (Pharmaceutical Affairs Act, Food Sanitation Act) as in the case of drugs.

Distribution of cosmetics generally has a shelf life of 1 to 3 years at room temperature, making them more vulnerable to microorganisms. Cosmetics are also highly vulnerable to microorganisms due to continuous contact with hands after opening. Therefore, a preservative with high preservative power must be used to block contamination from these microorganisms and ensure the safety and quality of the product. All cosmetics on the market contain preservatives to inhibit the growth and kill microorganisms, and contain phenoxyethanol, parabens, chlorophenicin, disodium EDTA, cetylpyridinium chloride, benzalkonium chloride, and silver. There are over 30 different types, including colloids. However, in the case of parabens, the demand began to decrease as research showed that they can cause breast cancer, and it is true that these synthetic chemical ingredients are harmful to the human body and that continuous exposure to the environment can lead to death in severe cases. Research on natural preservatives is being conducted steadily around the world in order to solve and replace the harmful problems of chemically synthesized preservatives. However, to date, no perfect natural preservative has been developed in the world, and only a few products with natural preservatives are available at home and abroad. was released, but this also had prohibited ingredients detected in the verification process or had no preservative effect in other strains except for the five major strains (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger), leading to product failure due to microbial contamination. It has major drawbacks.

Meanwhile, human skin undergoes various physical and chemical changes during the aging process, and the causes are largely divided into intrinsic aging and photo-aging, and research on these has been actively conducted. In other words, it can be caused by the activation of free radicals due to ultraviolet rays, stress, disease states, environmental factors, wounds, and aging. If this condition worsens, it destroys the antioxidant defense network that exists in the body and damages cells and tissues. This promotes adult diseases and aging. To be more specific, the major components of skin, such as lipids, proteins, polysaccharides, and nucleic acids, are oxidized, destroying skin cells and tissues, and ultimately causing skin aging. In particular, protein oxidation breaks down the skin's connective tissues, such as collagen, hyaluronic acid, elastin, proteoglycan, and fibronectin, causing severe excessive inflammatory reactions and disruption of skin elasticity. If this becomes more severe, it can lead to mutations, cancer, and decreased immune function due to DNA mutations.

Therefore, the cell membrane must be protected by scavenging free radicals generated during the body's metabolic process, ultraviolet irradiation, and free radicals mediated by inflammatory reactions, and already damaged cells must be regenerated through active metabolism to proliferate cells to maintain healthy skin. You can recover quickly and maintain healthy skin. As skin ages, it becomes thinner, loses elasticity, and falls in the direction of gravity. Wrinkles are caused not only by collagen, which keeps the skin matrix elastic, but also by a protein called elastin, which binds collagen together and supports it firmly. Even if collagen is abundant in the body, if elastin, which is a support, is absent, the skin collapses and loses elasticity without a framework to support collagen, and the absence of elastin is involved in the formation of wrinkles. Therefore, we attempted to prevent the formation of skin wrinkles by inhibiting cellular elastin-degrading enzymes.

Next, there are skin changes caused by pigmentation. Pigments that affect skin color include melanin, melanoid, carotene, and hemoglobin, the most important of which are melanin, and the biggest factors affecting its biosynthesis are ultraviolet rays and the level of hormone secretion in the body. Melanin plays a major role in preventing skin damage from ultraviolet rays by absorbing or scattering ultraviolet rays. It has no particular maximum absorption wavelength and absorbs light in all areas. In addition, the function of removing reactive oxygen species is excellent, but sometimes melanin itself generates reactive oxygen species, reduces or oxidizes other substances by catechol or quinone in the melanin structure, and melanin itself exhibits the properties of free radicals. I pray.

Caprylhydroxamic acid derived from coconut of the present invention is a component based on coconut oil derived from coconut, a light green tropical fruit that is the fruit of the cocos palm. The jelly-like flesh inside the coconut fruit has a sweet and savory taste and is eaten as is or squeezed for oil. When fully ripe, it turns brown and the flesh becomes hard. Caprylic hydroxamic acid, a coconut-derived substance, is soft and acts as an excellent preservative, and can be used as an alternative to harmful existing preservatives such as parabens. It can be used particularly effectively at neutral pH. It also acts as a stabilizer by blocking the activity of metal ions in cosmetics. Microorganisms that may cause harm have been shown to have reduced survival rates in metal-limited environments. Caprylic Hydroxamic Acid has excellent safety and can be applied to various formulations.

1,3-Butylene Glycol derived from sugar cane of the present invention is used in sugar cane, wine, aged cheese, rat liver, soil yeast, corn oil, sunflower oil, sheep oil, and fish liver oil. It is an ingredient contained in etc. Sugarcane is a perennial herb from the monocot plant family Rice Plantae and grows to a height of 2 to 6 m in tropical regions. The stems grow in groups, stand up straight, and do not branch. It also appears in alcohol fermentation and lactose fermentation products. It has moderate wettability, good solubility and antibacterial properties, is less irritating and toxic to the skin, and has a cleaner feel than glycerin and is less sticky, so it is widely used as a moisturizer, solvent, and fragrance retention agent in various creams, lotions, aerosol products, toothpaste, etc. there is. It is also applied to polyester-based reversible agents, polyvinyl chloride reversible agents, polyester resin raw materials, alkydo resin raw materials, and polyurethane raw materials. It is also used as a humectant in cigarettes, cellophane, and Vinylon films. It is also used as a solvent for subcutaneous injection drugs and as a feed additive.

Caprylyl glycol derived from coconut palm of the present invention is a component extracted from the oil of coconut, a light green tropical fruit, which is the fruit of the cocos palm. It moisturizes the skin in the form of a diol with a hydroxy group (-OH) attached. It is involved in maintenance and has the function of maintaining the stability of cosmetics, so it can be widely applied to a variety of products. It is an ingredient that acts as a natural preservative because it softens the skin and acts as an antibacterial agent. It may be used alone for its preservative effect, but it is commonly used by mixing with other preservatives to increase antibacterial power and antibacterial activity. Because caprylyl glycol has a high melting point, it exists as a white solid in cold winter, but is generally in a transparent liquid state. It also has the effect of gently caring for skin and hair.

The grape skin of the present invention is the outer skin of grapes, which are the fruit of a deciduous vine plant of the dicotyledonous buckthorn order Grape family. It is presumed that it was brought to Korea from China during the Goryeo Dynasty, and full-scale cultivation began in the 1900s. Grapes have tendrils, which, depending on the species, meet the leaves continuously or intermittently. It is about 3m long and has yellowish green flowers in May-June on dioecious or bisexual plants. The fruit is a fleshy fruit that ripens between August and October. The skin is dark purplish-black, red-red, yellowish-green, etc. The shape of the fruit is ball-shaped, oval-shaped, or cylindrical with pointed ends at both ends. Varies. It contains a lot of sugar (glucose, fructose), which is good for recovering from fatigue, and is rich in vitamins A, B, B2, C, and D, which facilitates metabolism. In addition, it also contains minerals such as calcium, phosphorus, iron, sodium, and magnesium. It is good for anemia by promoting blood production and hematopoiesis, prevents cavities by inhibiting viral activity, and contains a component called resveratrol, which has various effects such as antioxidant, anti-inflammatory, and anti-cancer properties. It also helps prevent degenerative diseases such as Alzheimer's disease and Parkinson's disease by improving the activity and efficacy of neural enzymes that create nerve cells.

The lemon of the present invention is the fruit of an evergreen tree of the dicotyledonous plant, Rutaceae, and is native to the Himalayas, where it grows well in places where the climate is relatively cool and free of change. It is widely cultivated in Italy, Spain, California in the United States, and Australia, but the best varieties are those grown along the Mediterranean coast. It grows up to 3~6m in height. The leaves are alternate and are red when young, but gradually turn green. Flowers bloom from May to October and hang one by one or in groups of several in the leaf axil. The flower buds are red, the inside of the flower is white, and the outside is a strong reddish purple. Fruits are harvested 6 to 10 times a year, mainly from October until the spring of the following year, with the largest harvest occurring in November and December. In California, it is harvested all year round, with the largest harvest occurring between January and May. The fruit is oval-shaped and has a green outer skin, but when ripe, it turns yellow and has a strong scent. Harvest and cook when the skin is green, before it is fully ripe. The seeds are egg-shaped and have sharp ends. It has a strong sour taste because it contains a lot of vitamin C and citric acid. Lemon oil is squeezed from the peel and used as a raw material for drinks, perfume, and lemonade, and the juice is used as a raw material for drinks, vinegar, and cosmetics, and is also used as a flavoring when making cookies.

The lavender of the present invention is a plant belonging to the genus Lavandula of the dicotyledonous plant order Lamiaceae and is native to the Mediterranean coast. The height is 30 to 60 cm, and if taken care of well in the garden, it can grow up to 90 cm. There are white hairs all over, and the stem is dull-square, clumped together, and has many branches at the bottom. The leaves are whorled or opposite, lanceolate, 4 cm long and 4 to 6 mm wide. There are no petioles and the leaves have fine hairs. Flowers bloom in light purple or white from June to September and grow sparsely in spikes at the end of long flower stalks without leaves. Between the hairs covering the flowers, leaves, and stems, there are oil glands that emit scent. It grows well in well-drained sandy soil mixed with a little gravel, and prefers soil that is not too fertile. It grows well in a south-facing location that receives plenty of sunlight and in a place that is not humid. It is cultivated to collect fragrant oil from flowers and plants, and is also planted for ornamental purposes. Fragrance oil is used as a raw material for perfumes and cosmetics, as a flavoring agent for cooking, and is also used to treat headaches and nervous disorders. The species Lavandula officnalis or Lavandula angustifolia are mainly used for aromatherapy. It is said that ancient Romans bathed with lavender in their bathtubs, and placed dried, fragrant flowers in drawers or closets. And in household books published during the Elizabethan era in England, lavender often appears as a sterilizing and insect repellent, and there is a record that Queen Elizabeth I liked sweets made from lavender. The language of flowers is ‘fidelity.’

Morus bark of the present invention is a medicinal material made from the root bark of the mulberry tree of the Moraceae family or plants of the same genus, and in China and Japan, only the mulberry tree is used in processed herbal medicine. Sang (桑), meaning mulberry tree, is Yak (桑), meaning a sacred tree in the East. It is said to be a combination of ) and tree mok (木). This was used to distinguish between the two because mulberry, the tree that silkworms eat, is as effective as sourwood. This medicine has a slightly peculiar odor, is sweet in taste, and is cold in nature. [Sweet and cold] Morus bark treats seawater and asthma caused by lung heat and has a diuretic effect. It is used for acute pyelonephritis and debilitating edema, has a blood pressure lowering effect, and is also used for nosebleeds and hemoptysis. It is also used for epidemic hepatitis. Pharmacological effects have been reported to include antitussive, diuretic, lowering blood pressure, sedation, analgesic, antipyretic, antispasmodic, and antibacterial effects. The appearance is semi-tubular or band-shaped and is sometimes thinly cut vertically. The outer surface is white or yellow-brown, and those with attached skin have yellow-brown skin that falls off easily, have many thin vertical wrinkles, and have a reddish-brown skin. The side cut horizontally is white or light brown and fibrous, and the inner side is dark yellow brown and flat.

The ivy leaf of the present invention is an evergreen vine of the dicotyledonous umbel family, Araliaceae. Widely distributed in Europe and North Africa, it was introduced into Korea as a foliage plant and distributed under the name Helix. In its native habitat, it grows up to 30m in height. The leaves are alternate, glossy, dark green, 10cm long, and split into 3 to 5 branches. The leaves on old branches are not split and are egg-shaped. Young branches and small peduncles have grayish-white star hairs (star-shaped hairs that are divided into several branches). The flowers are bisexual and grow in umbels. There are 5 petals, 5 stamens and 5 styles each, and 5 ovaries. There are several species of horticultural varieties, and the representative species, the tricolor variety, has large leaves with irregular light green and milky patterns on a green background, and the milky parts turn light red at low temperatures.

The tangerine of the present invention is a plant fruit tree belonging to the dicotyledonous plant order Rutaceae and Citrus subfamily. It is the general name for each species belonging to the genus Citrus, Persimmon, and Angiospermia among the citrus subfamily, as well as the cultivars derived from these three genera. As fruit trees, only tangerine types according to the citrus genus are grown. All tangerine species are evergreen shrubs or small trees and have thorns on their branches. The native land of citrus fruits extends to a wide area, including India, Myanmar, the Malay Peninsula, Indochina, China, Korea, and Japan. In particular, the eastern Himalayas and Assam regions and the upper reaches of the Yangtze River in China are the native land of important species. there is. It has been cultivated in these regions since ancient times. It is said that it has been cultivated in Korea for a long time, but no clear records can be found, and in the Japanese history, Higoguksa, it is recorded that tachibana was imported from the Three Han Dynasties. In 《Goryeo Sasega (高麗史世家)》, it is said that in 1052 (the 6th year of King Munjong's reign of Goryeo), the amount of tangerines offered as tribute from Tamra was increased to 100 bags. Afterwards, more records can be found during the Joseon Dynasty. In the Annals of King Sejong, there is a record that in 1426 (the 8th year of King Sejong's reign), citrons and potatoes were planted in Gyeongsang-do and the southern coastal region of Jeolla-do for trial cultivation. In 《Tamnaji Fruit Tree Summary》, there is a record that in 1526 (the 21st year of King Jungjong's reign), Lee Su-dong, a Jeju pastor, increased the number of protective posts guarding citrus orchards. In addition, records of tangerine cultivation can be found in many literature, but only about 10 types of native tangerines remain today. Since Korea is the northernmost of the world's citrus fruit growing areas, the cultivated varieties are mainly cold-tolerant tangerine trees introduced from Japan in 1911. In the early 1960s, only a part of Jeju Island, centered on Seogwipo, was known as Korea's only citrus production area, but as a result of many experimental cultivations, it has recently been grown in the entire Jeju Island below 200m above sea level and in Tongyeong, Goheung, Wando, Geoje, Namhae, and Geumsan in the southern region. Some citrus fruits are also grown in the country. However, citrus fruit production in Korea is limited in the number of cultivars, the cultivation area is small, and the production volume is low.

Patent No. 10-2259004 discloses a preservative composition for cosmetics containing Morus bark extract, Brussels sprout extract, soapnut extract, and herbaceous extract, and Patent No. 10-1533195 discloses a safe wet tissue composition using natural preservatives. Patent No. 10-1532005 discloses a natural preservative containing citron seed extract and a manufacturing method thereof, and Patent No. 10-2394531 discloses a preservative containing caprylic/capric glyceride and an external skin preparation composition containing the same. Patent No. 10-0782599 discloses a composite natural preservative, Patent No. 10-1793266 discloses a method for manufacturing a natural preservative using natural rock water and mandarin concentrate, and Patent No. 10-2066821 discloses a natural composite cosmetic preservative composition. In Patent No. 10-1427462, there is a disclosure of a complex natural preservative consisting of extracts of lotus root, Hagochoe, Sansa, and Mokkol, and a cosmetic composition containing the same as an active ingredient, and in Patent No. 10-1667357, there is a disclosure of a cosmetic composition containing plant extracts. Disclosure of a natural preservative composition, Patent No. 10-1776202 discloses a natural complex preservative composition and its manufacturing method, Patent No. 10-0615892 discloses a natural preservative containing mugwort extract, and Patent No. 10-1806800 discloses a natural preservative containing mugwort extract. Disclosure of complex natural preservatives containing extracts and their manufacturing methods; Patent No. 10-2272130 discloses preservative compositions for cosmetics using natural raw materials; and Patent No. 10-0723906 discloses natural preservatives and their manufacturing methods. , Patent No. 10-2146522 discloses a natural preservative composition and a cosmetic composition containing the same, Patent No. 10-1533195 discloses a safe wet tissue composition using natural preservatives, and Patent No. 10-1839612 discloses a plant complex extract as an active ingredient. Patent No. 10-1818146 discloses a natural preservative composition containing white willow bark, North American aspen bark, neem leaf, and blueberry extract.

However, to date, there are no patents or papers on the preservative effect, antioxidant, whitening, wrinkle improvement and soothing effect due to the synergistic effect of the plant-derived complex natural extract.

Therefore, as part of a study to develop a plant-derived preservative that is safer and has functional properties for improving skin health, as a result of testing the antioxidant activity of various natural products, the present inventors found that grape skin, lavender flower, and lemon have good antibacterial and antiviral activities. , Morus bark, ivy leaves, and tangerines were extracted and mixed at the optimal composition ratio, and it was confirmed that the synergistic effect resulted in excellent antibacterial ability and excellent antioxidant effect by effectively removing reactive oxygen species through various antioxidant mechanisms. In addition, it was confirmed that it has a whitening effect through inhibition of tyrosinase, which promotes the production of melanin, which is involved in skin pigmentation, and a wrinkle prevention effect by inhibiting the enzyme that decomposes elastin, which plays a role in binding the collagen that makes up the skin. By doing this, the present invention was completed.

Therefore, the purpose of the present invention is to provide a 100% plant-derived preservative composition with excellent antioxidant, whitening, wrinkle improvement, and soothing effects. More specifically, it relates to coconut-derived acids, sugar cane, and coconut palm-derived glycols. , a mixed composition containing grape skin extract, lemon extract, lavender extract, mortar bark extract, ivy leaf extract, and citrus extract as active ingredients is a 100% plant-derived preservative that has the effect of inhibiting various bacteria, yeast, and mold within a short period of time. In addition to its natural preservative role, it has excellent antioxidant, whitening, wrinkle improvement, soothing, and antibacterial effects. By confirming the remarkable effect of the mixed composition compared to individual ingredients and extracts, it is 100% excellent in antioxidant, whitening, wrinkle improvement, soothing, and antibacterial effects. To provide plant-derived preservative compositions and cosmetic compositions.

According to the present invention to achieve the above-described object, 79.0% by weight of 1,3-butylene glycol, 10.0% by weight of caprylhydroxamic acid, and caprylyl glycol A preservative containing 5.0% by weight of caprylyl glycol, 1.0% by weight of grape skin extract, 1.0% by weight of lavender flower extract, 1.0% by weight of lemon extract, 1.0% by weight of Morus bark extract, 1.0% by weight of ivy leaf extract, and 1.0% by weight of citrus extract. A composition is provided.

Here, the butylene glycol may be derived from sugar cane, the capryl hydroxamic acid may be derived from coconut, and the caprylyl glycol may be derived from palm or coconut.

In addition, the preservative composition includes the steps of mixing grape skin with 70% ethanol to extract grape skin extract; 99.0% by weight of a liquid containing 67.0% by weight, 30.0% by weight, and 3.0% by weight of purified water, ethanol, and methyl nonafluorobutyl ether-methyl nanofluoroisobutyl ether mixture, respectively, and Mixing and dissolving 1.0% by weight of the grape skin extract and then separating and purifying it under refrigerated conditions at 5°C for 2 days; Extracting extracts from 5.0% by weight of lavender flowers, lemon, linden bark, ivy leaves, and tangerines and 95.0% by weight of 1,3-butylene glycol at 80° C. for 2 hours, respectively; Filtering the extract with a 0.45μm filter after completion of extraction; and 79.0% by weight of 1,3-Butylene glycol, 10.0% by weight of Caprylhydroxamic acid, 5.0% by weight of Caprylyl glycol, grape skin. Mixing 1.0% by weight of extract, 1.0% by weight of lavender flower extract, 1.0% by weight of lemon extract, 1.0% by weight of Morus bark extract, 1.0% by weight of ivy leaf extract, and 1.0% by weight of citrus extract; It may be manufactured by a manufacturing method including.

In addition, a cosmetic composition for antioxidant, whitening, and wrinkle improvement comprising the preservative composition is provided.

According to the present invention as described above, the plant-derived preservative composition of the present invention has been confirmed to have excellent antibacterial and antiviral effects due to its synergistic effect, and in addition, it has excellent antioxidant properties and whitening and elastase inhibition through tyrosinase inhibition. Since it has additional functions such as wrinkle improvement effect, it can be used as a complex functional cosmetic preservative material that can help the skin.

The effects according to the present invention described above are not limited to the above, and other effects of the present invention will be clearly apparent to those skilled in the art through the examples and claims described later. It will be understandable.

Figure 1 shows the results of a DPPH scavenging test of the preservative composition of the present invention.
Figure 2 shows the results of the ABTS elimination test of the preservative composition of the present invention.
Figure 3 shows the SOAR test results of the preservative composition of the present invention.
Figure 4 shows the FRAP test results of the preservative composition of the present invention.
Figure 5 shows the results of a whitening effect (Tyrosinase inhibition) test of the preservative composition of the present invention.
Figure 6 shows the results of the wrinkle improvement effect (Elastase inhibition) test of the preservative composition of the present invention.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a plant-derived preservative composition having antioxidant, whitening, wrinkle-improving and soothing effects, and to a cosmetic composition containing the same, and more specifically, to caprylhydroxamic acid derived from coconut and 1 derived from sugarcane. , Contains 1,3-Butylene glycol, Caprylyl glycol derived from coconut palm, grape skin extract, lemon extract, lavender extract, Morus bark extract, ivy leaf extract, and citrus extract. The mixed composition is a 100% plant-derived preservative that has the effect of suppressing various bacteria, yeast, and mold, and has anti-oxidation, whitening, wrinkle improvement, soothing, and antibacterial effects in addition to preservative functions.

Specifically, the present invention relates to plant-derived 1,3-butylene glycol, caprylhydroxamic acid, caprylyl glycol, grape skin extract, and lavender. By combining beneficial active ingredients from flower extract, lemon extract, Morus bark extract, ivy leaf extract, and citrus extract, their synergistic effect not only acts as a preservative but also has excellent antioxidant, whitening, wrinkle improvement, and soothing effects compared to individual ingredients and extracts. The significantly superior effect of the composition used by mixing was confirmed.

The term “extract” used in the present invention refers to an extract obtained by extraction treatment of a dried sample, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, a crude product or purified product of the extract, or a mixture thereof, etc. This includes the extract itself and all formulations of the extract that can be formed using the extract.

The method for extracting the extract of the present invention is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of extraction methods that can be used in the present invention include hot water extraction, ultrasonic extraction, filtration, reflux extraction, and leaching, which can be performed alone or by combining two or more methods. and can preferably be performed using a cold immersion extraction method.

The type of extraction solvent used to extract the complex natural product of the present invention is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water, distilled water, ethanol, methanol, propanol, isopropanol, butanol, propanol, butylene glycol, propylene glycol, acetone, ether, benzene, chloroform, dichloromethane, ethyl acetate, and methylene. One or more solvents selected from the group consisting of chloride, n-hexane, hydrochloric acid, acetic acid, formic acid, diethyl ether, petroleum ether and cyclohexane, and preferably, when alcohol is used as the solvent, it has 1 carbon atom. Alcohols from 4 to 4 (methanol, ethanol, propanol, butanol) can be used.

The cosmetic composition of the present invention can be prepared including the preservative composition of the present invention described above, a cosmetically effective amount, and a cosmetically acceptable carrier.

The appearance of the cosmetic composition contains a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, such as solutions, gels, solids, pasty anhydrous products, emulsions obtained by dispersing the oil phase in the water phase, suspensions, microemulsions, microcapsules, microgranules or ionic forms (liposomes) and It may be provided in the form of a non-ionic vesicular dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray or concealer stick. These compositions can be prepared according to conventional methods in the art. The composition according to the invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The cosmetic composition according to an embodiment of the present invention is not particularly limited in its formulation, and for example, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream. It may be one or more formulations selected from formulations consisting of nutritional cream, moisture cream, hand cream, foundation, essence, nutritional essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier ingredient. You can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier component. In particular, when the formulation is a spray, chlorofluorohydrocarbon and propane may be used as carrier ingredients. /May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, and fatty acid amide. Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative, or ethoxylated glycerol fatty acid ester can be used.

The cosmetic composition according to an embodiment of the present invention may further include functional additives and components included in general cosmetic compositions in addition to the preservative composition. The functional additive may include ingredients selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extract.

In addition to the above-mentioned functional additives, the cosmetic composition of the present invention may also contain components included in general cosmetic compositions, if necessary. Other ingredients included include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, disinfectants, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation promoters, Cooling agents, antiperspirants, or purified water may be used.

The dried sample of the composite natural product of the present invention contains 0.01 to 20.0% by weight of grape skin, 0.01 to 20.0% by weight of lavender flowers, 0.01 to 20.0% by weight of lemon, 0.01 to 20.0% by weight of mortar bark, and 0.01% by weight of ivy leaves, based on their respective dry weights. It is available in ~10.0% by weight and 0.01~10.0% by weight of tangerine.

When combined with the natural extract obtained through the above composition and process, it exhibits excellent preservative, antioxidant, whitening, and wrinkle-improving effects.

The preservative composition of the present invention contains 79.0% by weight of 1,3-Butylene glycol, 10.0% by weight of Caprylhydroxamic acid, and 5.0% by weight of Caprylyl glycol. Weight% may include 1.0% by weight of grape skin extract, 1.0% by weight of lavender flower extract, 1.0% by weight of lemon extract, 1.0% by weight of Morus bark extract, 1.0% by weight of ivy leaf extract, and 1.0% by weight of tangerine extract.

Here, butylene glycol is preferably derived from sugar cane, the capryl hydroxamic acid is derived from coconut, and the caprylyl glycol is preferably derived from palm or coconut.

In the 100% plant-derived preservative composition with excellent antioxidant, whitening, wrinkle improvement, soothing, and antibacterial effects of the present invention, the content of the complex natural extract is preferably 0.0001 to 10% by weight based on the total weight of the cosmetic composition, and this complex natural extract Cosmetic compositions containing can be manufactured in various formulations such as lotions, essences, creams, packs, patches, skin adhesive gels, foundations, and makeup bases, and can be applied to conventional cosmetic manufacturing methods.

Specifically, it can be applied in various forms such as liquid, cream, paste, and solid, and may contain various common auxiliaries and carriers that are suitable for each of these formulations and are well known in the art.

The 100% plant-derived preservative composition of the present invention has excellent antibacterial and antiviral effects as a preservative for cosmetics. Unlike existing cosmetic preservative ingredients, it does not contain synthetic chemicals and has secondary effects of antioxidant, whitening, and wrinkle improvement. .

Hereinafter, to help understand the present invention, specific examples such as examples, comparative examples, and test examples will be described. However, the following examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following examples.

<Example 1>

After washing thoroughly, the dried grape skins were mixed with 70% ethanol and the grape skin extract was extracted for 12 hours. In order to remove the color and odor of resveratrol, the active ingredient obtained through grape skin extraction, purified water, ethanol, and methyl nanofluorobutyl ether-methyl nanofluoroisobutyl ether were used. ether) mixture (Novec 7100, 3M, USA) at 67.0 wt%, 30.0 wt%, and 3.0 wt%, respectively, and 1.0 wt% of the grape skin extract was dissolved in 99.0 wt% of the mixture and then incubated at 5°C for 2 days. It was separated, purified, and recrystallized under refrigerated conditions. Mix dried lavender flowers, lemon, bark, ivy leaves, and tangerines with 5.0% by weight each and 95.0% by weight of 1,3-butylene glycol, a natural substance, at 80°C for 2 hours. It was extracted during. The extract was mixed after completion of extraction and filtered through a 0.45μm filter. Then, this filtrate and coconut, sugar cane, and coconut oil-derived substances such as 1,3-Butylene glycol, Caprylhydroxamic acid, and caprylyl glycol (Caprylyl glycol) to prepare the final 100% plant-derived preservative composition sample. The final mixing ratio is 79.0% by weight of 1,3-butylene glycol, 10.0% by weight of caprylic hydroxamic acid, 5.0% by weight of caprylyl glycol, 1.0% by weight of grape skin extract, 1.0% by weight of lavender flower extract, and lemon. It consisted of 1.0% by weight of extract, 1.0% by weight of Morus bark extract, 1.0% by weight of ivy leaf extract, and 1.0% by weight of citrus extract.

<Comparative Example 1>

After washing thoroughly, the dried grape skins were mixed with 70% ethanol and the grape skin extract was extracted for 12 hours. In order to remove the color and odor of resveratrol, the active ingredient obtained through grape skin extraction, purified water, ethanol, and methyl nanofluorobutyl ether-methyl nanofluoroisobutyl ether were used. ether) mixture (Novec 7100, 3M, USA) at 67.0 wt%, 30.0 wt%, and 3.0 wt%, respectively, and 1.0 wt% of the grape skin extract was dissolved in 99.0 wt% of the mixture and then incubated at 5°C for 2 days. It was separated, purified, and recrystallized under refrigerated conditions. Mix dried lavender flowers, lemon, bark, ivy leaves, and tangerines with 5.0% by weight each and 95.0% by weight of 1,3-butylene glycol, a natural substance, at 80°C for 2 hours. It was extracted during. The extract was mixed after completion of extraction and filtered through a 0.45μm filter. Then, this filtrate and coconut, sugar cane, and coconut oil-derived substances such as 1,3-Butylene glycol, Caprylhydroxamic acid, and caprylyl glycol (Caprylyl glycol) to prepare the final 100% plant-derived preservative composition sample. The final mixing ratio is 79.0% by weight of 1,3-butylene glycol, 6.0% by weight of capryl hydroxamic acid, 5.0% by weight of caprylyl glycol, 2.0% by weight of grape skin extract, and 1.0% by weight of lavender flower extract. It consisted of 1.0% by weight of lemon extract, 2.0% by weight of Morus bark extract, 2.0% by weight of ivy leaf extract, and 2.0% by weight of tangerine extract.

<Comparative Example 2>

After washing thoroughly, the dried grape skins were mixed with 70% ethanol and the grape skin extract was extracted for 12 hours. In order to remove the color and odor of resveratrol, the active ingredient obtained through grape skin extraction, purified water, ethanol, and methyl nanofluorobutyl ether-methyl nanofluoroisobutyl ether were used. ether) mixture (Novec 7100, 3M, USA) at 67.0 wt%, 30.0 wt%, and 3.0 wt%, respectively, and 1.0 wt% of the grape skin extract was dissolved in 99.0 wt% of the mixture and then incubated at 5°C for 2 days. It was separated, purified, and recrystallized under refrigerated conditions. Mix 5.0% by weight of lavender flowers, lemon, bark, ivy leaves, and tangerines with 95.0% by weight of 1,3-butylene glycol, a natural substance, and extract at 80°C for 2 hours. did. The extract was mixed after completion of extraction and filtered through a 0.45μm filter. Then, this filtrate and coconut, sugar cane, and coconut oil-derived substances such as 1,3-Butylene glycol, Caprylhydroxamic acid, and caprylyl glycol (Caprylyl glycol) to prepare the final 100% plant-derived preservative composition sample. The final mixing ratio is 79.0% by weight of 1,3-butylene glycol, 7.0% by weight of caprylic hydroxamic acid, 5.0% by weight of caprylyl glycol, 2.0% by weight of grape skin extract, 1.0% by weight of lavender flower extract, and lemon. It consisted of 1.0% by weight of extract, 1.0% by weight of Morus bark extract, 2.0% by weight of ivy leaf extract, and 2.0% by weight of citrus extract.

<Comparative Example 3>

After washing thoroughly, the dried grape skins were mixed with 70% ethanol and the grape skin extract was extracted for 12 hours. Resveratrol, the active ingredient obtained through grape skin extraction, was mixed with purified water, ethanol, and methyl nonafluorobutyl ether to remove the color and odor. ) 1.0% by weight of the grape skin extract was dissolved in 99.0% by weight of the mixture (Novec 7100, 3M, USA) at 67.0% by weight, 30.0% by weight, and 3.0% by weight, respectively, and incubated at 5°C for 2 days. It was separated, purified, and recrystallized under refrigerated conditions. Mix 5.0% by weight of lavender flowers, lemon, bark, ivy leaves, and tangerines with 95.0% by weight of 1,3-butylene glycol, a natural substance, and extract at 80°C for 2 hours. did. The extract was mixed after completion of extraction and filtered through a 0.45μm filter. Then, this filtrate and coconut, sugar cane, and coconut oil-derived substances such as 1,3-Butylene glycol, Caprylhydroxamic acid, and caprylyl glycol (Caprylyl glycol) to prepare the final 100% plant-derived preservative composition sample. The final mixing ratio is 79.0% by weight of 1,3-butylene glycol, 8.0% by weight of caprylic hydroxamic acid, 5.0% by weight of caprylyl glycol, 2.0% by weight of grape skin extract, 1.0% by weight of lavender flower extract, and lemon. It consisted of 1.0% by weight of extract, 1.0% by weight of Morus bark extract, 1.0% by weight of ivy leaf extract, and 2.0% by weight of citrus extract.

<Comparative Example 4>

After washing thoroughly, the dried grape skins were mixed with 70% ethanol and the grape skin extract was extracted for 12 hours. In order to remove the color and odor of resveratrol, the active ingredient obtained through grape skin extraction, purified water, ethanol, and methyl nanofluorobutyl ether-methyl nanofluoroisobutyl ether were used. ether) mixture (Novec 7100, 3M, USA) at 67.0 wt%, 30.0 wt%, and 3.0 wt%, respectively, and 1.0 wt% of the grape skin extract was dissolved in 99.0 wt% of the mixture and then incubated at 5°C for 2 days. It was separated, purified, and recrystallized under refrigerated conditions. Mix 5.0% by weight of lavender flowers, lemon, bark, ivy leaves, and tangerines with 95.0% by weight of 1,3-butylene glycol, a natural substance, and extract at 80°C for 2 hours. did. The extract was mixed after completion of extraction and filtered through a 0.45μm filter. Then, this filtrate and coconut, sugar cane, and coconut oil-derived substances such as 1,3-Butylene glycol, Caprylhydroxamic acid, and caprylyl glycol (Caprylyl glycol) to prepare the final 100% plant-derived preservative composition sample. The final mixing ratio is 79.0% by weight of 1,3-butylene glycol, 9.0% by weight of caprylic hydroxamic acid, 5.0% by weight of caprylyl glycol, 2.0% by weight of grape skin extract, 1.0% by weight of lavender flower extract, and lemon. It consisted of 1.0% by weight of extract, 1.0% by weight of Morus bark extract, 1.0% by weight of ivy leaf extract, and 1.0% by weight of citrus extract.

<Test Example 1> Antibacterial test

The strains used to measure the antibacterial effect were Gram-positive bacteria ( Staphylococcus aureus ATCC 6538P), Gram-negative bacteria ( Escherichia coli ATCC 8739) and Pseudomonas aeruginosa ATCC 9027, and yeast ( Candida albicans ATCC). 10231), and Aspergillus niger ATCC 22343) was used as a filamentous fungus obtained from the Korea Research Institute of Bioscience and Biotechnology Biological Resources Center (KCTC, Korea). Three types of bacteria, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, are first cultured in broth medium at room temperature sterilized at 121°C for 15 minutes in a high-pressure steam sterilizer, then incubated at 37°C for 72 hours, and then subcultured five times. It was stabilized after an adaptation period. Fungi such as Candida yeast and Black Aspergillus were used in experiments after stabilizing them under culture conditions by incubating them at 24°C for 120 hours and subculturing them five times. ^Bacteria were inoculated at ¹ The number of bacteria was checked over time to determine how much had died from the initial inoculation dose to determine whether the preservative prescription was appropriate. For bacteria, a bacterial count of 10 cfu/ml or less after 7 days, and for fungi, a count of 1 × 10 ³ cfu/ml or less was considered appropriate. The test results numerically expressed the growth degree of the five types of bacteria in Tables 1 to 5 below. As shown in Table 1, it was confirmed that the growth of Staphylococcus aureus, one of the five major strains, was completely inhibited and killed in the four test groups of Examples and Comparative Examples after 120 hours. As shown in Table 2, in the case of E. coli, it can be confirmed that all bacteria were killed 120 hours after inoculation, except for Comparative Examples 1 and 2. As Table 3 shows, death of 40,000 bacteria in Example 1 and Comparative Example was observed 120 hours after inoculation of the test group with respect to the Pseudomonas aeruginosa strain, and complete death of the bacteria in the remaining test groups was confirmed after 168 hours. As shown in Tables 4 and 5, none of the test groups showed antibacterial efficacy except for Example 1 for 168 hours after inoculation in terms of killing yeasts and molds such as Candida yeast and Black Aspergillus.

In other words, the composition of the plant-derived complex extract corresponding to Example 1 has an antibacterial effect on all five major strains, and a strong killing effect on bacteria such as Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa was confirmed.

hour
test group Staphylococcus aureus ATCC 6538P
Anti-microbial Effect (cfu/㎖) inoculation 4 hours 24 hours 48 hours 120 hours 168 hours Example 1 1.0 × 10 ⁶ 3.8 × 10 ⁴ 5.1 × 10 ² 2.9 × 10 ² < 10 < 10 Comparative Example 1 1.0 × 10 ⁶ 3.7 × 10 ⁴ 5.6 × 10 ² 1.2 × 10 ² < 10 < 10 Comparative Example 2 1.0 × 10 ⁶ 2.1 × 10 ⁵ 5.2 × 10 ³ 3.0 × 10 ² < 10 < 10 Comparative Example 3 1.2 × 10 ⁶ 5.6 × 10 ⁴ 5.8 × 10 ² 5.3 × 10 < 10 < 10 Comparative Example 4 1.0 × 10 ⁶ 3.8 × 10 ⁴ 5.1 × 10 ² 2.9 × 10 ² < 10 < 10

hour
test group Escherichia coli ATCC 8739
Anti-microbial Effect (cfu/㎖) inoculation 4 hours 24 hours 48 hours 120 hours 168 hours Example 1 1.1 × 10 ⁶ 3.0×10 ³ 1.1 × 10 ³ 3.6 × 10 ² < 10 < 10 Comparative Example 1 1.1 × 10 ⁶ 5.5×10 ³ 2.9 × 10 ³ 4.2 × 10 ² 1.1 × 10 ² < 10 Comparative Example 2 1.1 × 10 ⁶ 6.1×10 ³ 4.3 × 10 ² 3.8 × 10 ² 1.1 × 10 < 10 Comparative Example 3 1.1 × 10 ⁶ 3.0×10 ³ 1.1 × 10 ³ 3.6 × 10 ² < 10 < 10 Comparative Example 4 1.0 × 10 ⁶ 6.2×10 ³ 1.1 × 10 ³ 3.7 × 10 ² < 10 < 10

hour
test group Pseudomonas aeruginosa ATCC 9027
Anti-microbial Effect (cfu/㎖) inoculation 4 hours 24 hours 48 hours 120 hours 168 hours Example 1 1.1 × 10 ⁶ 9.7 × 10 ⁴ 5.4 × 10 ² 1.8 × 10 < 10 < 10 Comparative Example 1 1.3 × 10 ⁶ 7.8 × 10 ⁵ 8.8 × 10 ⁴ 5.5 × 10 ³ 1.2 × 10 ³ < 10 Comparative Example 2 1.1 × 10 ⁶ 2.9 × 10 ⁵ 1.2 × 10 ⁴ 2.5 × 10 ³ 3.3 × 10 ² < 10 Comparative Example 3 1.1 × 10 ⁶ 2.8 × 10 ⁵ 8.6 × 10 ⁴ 1.6 × 10 ⁴ 1.2 × 10 < 10 Comparative Example 4 1.0 × 10 ⁶ 3.4 × 10 ⁵ 1.8 × 10 ⁴ 2.9 × 10 ³ < 10 < 10

hour
test group Candida albicans ATCC 10231
Anti-microbial Effect (cfu/㎖) inoculation 4 hours 24 hours 48 hours 120 hours 168 hours Example 1 1.1 × 10 ⁶ 3.8 × 10 ⁵ 1.3 × 10 ⁴ 1.5 × 10 ³ 1.8 × 10 ² < 10 Comparative Example 1 1.0 × 10 ⁶ 3.5 × 10 ⁵ 2.6 × 10 ⁴ 1.4 × 10 ⁴ 4.3 × 10 ³ 2.0 × 10 ³ Comparative Example 2 1.0 × 10 ⁶ 5.0 × 10 ⁵ 4.1 × 10 ⁴ 1.8 × 10 ⁴ 3.8 × 10 ³ 2.2 × 10 ³ Comparative Example 3 1.0 × 10 ⁶ 3.2 × 10 ⁵ 4.5 × 10 ⁴ 1.0 × 10 ⁴ 2.3 × 10 ³ 1.8 × 10 ³ Comparative Example 4 1.1 × 10 ⁶ 3.3 × 10 ⁵ 4.3 × 10 ⁴ 6.6 × 10 ³ 2.2 × 10 ³ 1.4 × 10 ³

hour
test group Aspergillus niger ATCC 22343
Anti-microbial Effect (cfu/㎖) inoculation 4 hours 24 hours 48 hours 120 hours 168 hours Example 1 1.1 × 10 ⁶ 4.1 × 10 ⁵ 7.9 × 10 ⁴ 4.6 × 10 ³ 2.3 × 10 ² < 10 Comparative Example 1 1.1 × 10 ⁶ 5.6 × 10 ⁵ 6.6 × 10 ⁴ 2.9 × 10 ³ 2.6 × 10 ³ 1.5 × 10 ³ Comparative Example 2 1.0 × 10 ⁶ 3.6 × 10 ⁵ 6.7 × 10 ⁴ 5.9 × 10 ³ 4.0 × 10 ³ 3.3 × 10 ³ Comparative Example 3 9.6 × 10 ⁵ 6.2 × 10 ⁴ 2.5 × 10 ⁴ 1.7 × 10 ⁴ 2.1 × 10 ³ 1.1 × 10 ³ Comparative Example 4 1.0 × 10 ⁶ 5.0 × 10 ⁴ 3.7 × 10 ⁴ 1.4 × 10 ⁴ 1.8 × 10 ³ 1.1 × 10 ³

<Test Example 2> Antioxidant test method - DPPH free radical scavenging

Anti-aging functional efficacy was evaluated using the DPPH free radical scavenging test. The 100% plant-derived preservative composition corresponding to Example 1 was used in experiments by preparing samples diluted in solvent to 0.125%, 0.25%, 0.5%, 1.0%, and 2.0%. DPPH solution was prepared at 0.2mM and placed in a test tube at a ratio of 1:1 with the sample (100ul each), reacted under light-blocked conditions for 15 minutes, and then the absorbance was measured using a micro plate reader at 540nm. Ascorbic acid 200μg/ml (0.02%), which is already known to have excellent antioxidant effects, was used as a positive control. Antioxidant efficacy was evaluated using the measured absorbance using the method shown in Equation 1 below.

[Calculation Formula 1]

DPPH free radical scavenging activity (%): (1 - A/B))*100

A: Absorbance after reaction of sample

B: Absorbance after reaction of blank sample

DPPH Radical scavenging activity Plant-derived preservative composition Ascorbic
acid Administration concentration
(%) 0.125 0.25 0.5 1.0 2.0 0.02 Eradication rate (%) 12.29 19.32 37.64 51.66 85.9 82.18%

As shown in Table 6, as the content of the 100% plant-derived preservative composition increased, the reactive oxygen species scavenging rate tended to increase in a concentration-dependent manner (Figure 1). In addition, at a concentration of 2%, stronger antioxidant ability was confirmed than that of Ascorbic acid 0.02% used as a positive control.

<Test Example 3> Antioxidant test method - ABTS Radical elimination

Anti-aging functionality was evaluated through ABTS antioxidant activity evaluation. The difference between the ABTS and DPPH test methods is that DPPH is advantageous for evaluating the antioxidant activity of lipophilic substances and is easy to prepare for the test, while ABTS has the advantage of being able to measure both lipophilic and hydrophilic substances, but the test is time-consuming. The downside is that it is relatively large. The 100% plant-derived preservative composition corresponding to Example 1 used in the test was diluted in a solvent at five concentrations (0.125%, 0.25%, 0.5%, 1.0%, 2.0%). Ascorbic acid, 200 μg/ml (0.02%), which is already known to have excellent antioxidant activity, was used as a positive control. ABTS Stock solution was prepared by mixing 7.4mM of 2,2-azino-bis and 2.6mM of Potassium persulphate at a ratio of 1:1 (v/v) and inducing a reaction under light blocking conditions for 24 hours. Afterwards, the ABTS stock solution was diluted to measure an absorbance of 1.0 at 735 nm absorbance conditions using a micro plate reader, and this solution was used as the ABTS solution used in the test. 20ul of the sample and 180ul of ABTS Solution were stirred and left at room temperature for 10 minutes to induce a reaction for antioxidant evaluation. Afterwards, the absorbance was measured at 735 nm to obtain a value, and then the degree of antioxidant activity was calculated using the method shown in Equation 2 below.

[Calculation Formula 2]

ABTS radical scavenging activity (%): (1 - A/B))*100

A: Absorbance after reaction of sample

B: Absorbance after reaction of blank sample

ABTS Radical scavenging activity Plant-derived preservative composition Ascorbic
acid Administration concentration
(%) 0.125 0.25 0.5 1.0 2.0 0.02 Eradication rate (%) 23.09 34.33 52.38 62.67 87.41 81.22

As shown in Table 7, when the antioxidant ability of a 100% plant-derived preservative composition was confirmed through the ABTS test method, the clearance rate increased in a concentration-dependent manner depending on the administered concentration, and although there was a difference in value from DPPH Radical scavenging acitvity, the same pattern of antioxidant activity was observed. The effect was confirmed (Figure 2). Through secondary verification of the antioxidant effect using the ABTS test method, the strong antioxidant ability of the 100% plant-derived preservative composition was confirmed.

<Test Example 4> Antioxidant test method - SOAR

SOD is a type of antioxidant enzyme that reduces superoxide, a peroxide produced within living organisms, and serves to remove active oxygen, a highly oxidizing toxic substance that damages biological tissues and cells. The antioxidant effect of the 100% plant-derived preservative composition was confirmed through the SOD activity test method, a type of antioxidant enzyme that decomposes free radicals. Superoxide anion scavenging ability (SOAR) was measured using the Nitro blue tetrazolium (NBT) reduction method. The sample used in the test, a 100% plant-derived preservative composition corresponding to Example 1, was diluted in a solvent at five concentrations (0.125%, 0.25%, 0.5%, 1.0%, 2.0%). Ascorbic acid 200μl/ml (0.02%) was used as a positive control, and the experimental method was as follows. Superoxide anion radical is generated by reacting xanthine oxidase (0.049 U/ml) with 0.1M PBS (pH 8.0) containing xanthine (0.4mM/L), and O ² - produced by adding 0.25mM/L of NBT. It was allowed to react and generate, and the sample was administered simultaneously to measure the effect of the sample on eliminating superoxide anion. If the sample exhibits superoxide anion scavenging ability, the degree of reduction of NBT by O ² - is inhibited and the intensity of color development is reduced. The fluorescence was measured at 560 nm using a fluorescence spectrophotometer. Superoxide anion scavenging ability was expressed according to the following calculation formula 3.

[Calculation Formula 3]

Superoxide anion radical scavenging activity (%) = {(B-B’)-(A-A’)}/(B-B’)*100

A: Absorbance of sample 0.1ml + NBT 1.0ml + xanthine oxidase enzyme solution 1.0ml mixture

B: Absorbance of the mixture of 0.1ml purified water + 1.0ml NBT + 1.0ml xanthine oxidase enzyme solution

A’: Absorbance of 0.1ml sample + 1.0ml NBT

B’: Absorbance of 0.1ml purified water + 1.0ml NBT

SOAR activity Plant-derived preservative composition Ascorbic
acid Administration concentration
(%) 0.125 0.25 0.5 1.0 2.0 0.02 activation (%) 7.18 18.01 23.63 54.62 82.28 76.28

As shown in Table 8, as a result of treating 0.125%, 0.25%, 0.5%, 1.0%, and 2.0% of the 100% plant-derived preservative composition, the respective activities were 7.18%, 18.01%, 23.63%, 54.62%, It was found to be 82.28%. The maximum concentration of 100% plant-derived preservative composition, 2.0%, was known to have excellent antioxidant effect and showed higher activity than that administered at 0.02% concentration of ascorbic acid, a substance used as a positive control (Figure 3). Through this, it was confirmed that the 100% plant-derived preservative composition exhibits an antioxidant effect through a mechanism that enhances the activity of superoxide dismutase, one of the enzymes that decomposes free radicals.

<Test Example 5> Antioxidant test method - FRAP

The antioxidant activity of the 100% plant-derived preservative composition corresponding to Example 1 was confirmed through the Ferric reducing ability of plasma (FRAP) test. The FRAP test method is a method of measuring the antioxidant capacity of a sample through the conversion process of ferric ion to ferrous. The 100% plant-derived preservative composition corresponding to Example 1 was diluted in a solvent at five concentrations of 0.125%, 0.25%, 0.5%, 1.0%, and 2.0% and used as a sample. FRAP Solution was prepared by mixing 300mM acetate buffer (pH 3.6): 10mM TPTZ (2,4,6-tripyridyl-s-triazine): 20mM FeCl _3· 6H ₂ O in a ratio of 10:1:1. After adding 3 ml of FRAP Solution to five test tubes, 0.1 ml of each of the 100% plant-derived preservative compositions corresponding to Example 1 in five different concentrations diluted in solvents and 0.3 ml of distilled water were added and placed in an oven at 37°C for 5 minutes. After reaction, the absorbance was measured at 590 nm. As a positive control, Trolox 10 μg/ml (0.001%) was used, and the antioxidant effect was calculated using Equation 4 below.

[Calculation Equation 4]

FRAP radical scavenging activity (%) = (1 - (A/B))*100

A: Absorbance after reaction of sample solution

B: reaction absorbance of blank sample

FRAP scavenging activity Plant-derived preservative composition Trolox Administration concentration
(%) 0.125 0.25 0.5 1.0 2.0 0.001 Inhibition rate (%) 11.48 26.99 43.78 66.61 83.57 52.34

As shown in Table 9, the antioxidant capacity of the 100% plant-derived preservative composition measured through the FRAP test method is 66.61%, which is higher than the antioxidant rate of 52.34% when 0.001% of Trolox, used as a positive control, was administered from an administration concentration of 1.0%. %, and at an administered concentration of 2.0%, a very high antioxidant capacity of 83.57% was confirmed (Figure 4). In summary, through four tests to measure antioxidant capacity, it was confirmed that the 100% plant-derived preservative composition is a material that can provide antioxidant effects through various mechanisms.

<Test Example 6> Whitening activity test method - Tyrosinase inhibitory activity test method

Whitening functionality was evaluated through evaluation of tyrosinase inhibitory activity. The test method was conducted by referring to the guidelines for evaluating the effectiveness of functional cosmetics that help whiten the skin notified by the Ministry of Food and Drug Safety. The method announced by the Ministry of Food and Drug Safety is as follows. The sample was completely dissolved in a solvent (ethyl alcohol), and then five concentrations were prepared to evaluate the ability to inhibit Tyrosinase activity. 220 μl of 0.1M phosphate buffer solution (PBS, pH 6.5 ± 0.5), 20 μl of sample solution, and 20 μl of mushroom tyrosinase solution (1500-2000 U/ml) were sequentially administered to the test tube. 40 μl of 1.5mM tyrosine solution was added to this solution, reacted at 37°C for 10 to 15 minutes, and absorbance was measured at 490 nm. Instead of the sample solution, the solvent in which the sample was dissolved was used as a blank sample solution for correction, and the level of activity of the sample was compared using arbutin as a positive control. Tyrosinase inhibitory activity was calculated using equation 4 below.

[Calculation Equation 4]

Tyrosinase activity inhibition rate (%) = (100 - (B-B‘)/(A-A’))*100

A: Absorbance after reaction of blank sample solution

B: Absorbance after reaction of sample solution

A’, B’: Absorbance measured by replacing Tyrosinase with buffer solution

Tyrosinase inhibition activity Plant-derived preservative composition Arbutin Administration concentration
(%) 0.125 0.25 0.5 1.0 2.0 0.04 Inhibition rate (%) 26.78 43.19 61.33 72.06 78.47 54.11

As shown in Table 10, it was confirmed that as the administered concentration of the 100% plant-derived preservative composition increased, Tyrosinase, which is involved in skin pigmentation, was inhibited. When the administration concentration of the 100% plant-derived preservative composition was 0.5%, the Tyrosinase inhibition rate was higher than when Arbutin, a substance that helps whitening, was administered at 0.04%, and was measured at a maximum of 78% at the concentration of 2.0% (Figure 5). Through this, it was found that the 100% plant-derived preservative composition is a whitening functional material with excellent Tyrosinase inhibitory activity even at low concentrations.

<Test Example 7> Wrinkle improvement activity test method - Elastase inhibition activity test method

Wrinkle improvement functionality was evaluated through evaluation of elastase inhibitory activity. Vegan preser used in the experiment was 0.125%, 0.25%, and 0.5%. The concentrations were adjusted to 1.0% and 2.0% and the respective activities were compared. Elastase inhibitory activity was determined by adding 100 μl of N-succinyl-(Ala)3-p-nitroamilide 1.1mM substrate to 10mM Tris-HCl buffer (pH 8.0) and then dissolving Elastase in 10mM Tris-HCl buffer (pH 8.0). 15 μl of 0.4 U/ml enzyme of porcine pancreas type IV was added and reacted at 37°C for 20 minutes, and then the absorbance was measured at 450 nm using a micro plate reader. Oleanolic acid 10 μg/ml was used as a positive control, and the solvent used to dissolve the sample was used as a blank sample. Elastase inhibitory activity was calculated by calculating the inhibition rate using Equation 5 below.

[Calculation Equation 5]

Elastase activity inhibition rate (%) = (100 - (B-B‘)/(A-A’))*100

A: Absorbance after reaction of blank sample solution

B: Absorbance after reaction of sample solution

A’, B’: Absorbance measured by replacing Tyrosinase with buffer solution

Elastase inhibition activity Plant-derived preservative composition Oleanolic acid Administration concentration
(%) 0.125 0.25 0.5 1.0 2.0 0.001 Inhibition rate (%) 12.75 26.47 45.11 60.98 83.72 42.51

As shown in Table 11 above, it was confirmed that as the administered concentration of the 100% plant-derived preservative composition increased, Elastase, which causes skin wrinkles, was significantly inhibited. Similar to the Tyrosinase inhibitory activity results, when the administered concentration of the 100% plant-derived preservative composition was 0.5%, the Elastase inhibition rate was higher than when 0.001% Oleanolic acid used as a positive control was administered, and the maximum was measured to be 78% at the 2.0% administered concentration. (Figure 6). Through this, it appears that the 100% plant-derived preservative composition can help improve wrinkles by preventing the development of wrinkles by preventing the skin's elasticity from collapsing through excellent elastase inhibition at low concentrations.

Claims (4)

1,3-Butylene glycol 79.0% by weight, Caprylhydroxamic acid 10.0% by weight, Caprylyl glycol 5.0% by weight, grape skin extract 1.0 A preservative composition comprising 1.0% by weight of lavender flower extract, 1.0% by weight of lemon extract, 1.0% by weight of Morus bark extract, 1.0% by weight of ivy leaf extract, and 1.0% by weight of citrus extract.
According to paragraph 1,
A preservative composition, characterized in that the butylene glycol is derived from sugar cane, the capryl hydroxamic acid is derived from coconut, and the caprylyl glycol is derived from palm or coconut.
According to paragraph 1,
The preservative composition is,
Extracting grape skin extract by mixing grape skin with 70% ethanol for 12 hours;
99.0% by weight of a liquid containing 67.0% by weight, 30.0% by weight, and 3.0% by weight of purified water, ethanol, and methyl nonafluorobutyl ether-methyl nanofluoroisobutyl ether mixture, respectively; Mixing and dissolving the grape skin extract at 1.0% by weight, then separating and purifying the grape skin extract under refrigerated conditions at 5° C. for 2 days;
Extracting extracts from lavender flowers, lemons, coriander bark, ivy leaves, and tangerines with 5.0% by weight and 95.0% by weight of 1,3-butylene glycol, respectively, at 80°C for 2 hours;
Filtering the extract with a 0.45μm filter after completion of extraction; and
1,3-Butylene glycol 79.0% by weight, Caprylhydroxamic acid 10.0% by weight, Caprylyl glycol 5.0% by weight, grape skin extract Mixing 1.0% by weight, 1.0% by weight of lavender flower extract, 1.0% by weight of lemon extract, 1.0% by weight of Morus bark extract, 1.0% by weight of ivy leaf extract, and 1.0% by weight of tangerine extract;
A preservative composition, characterized in that it is manufactured by a manufacturing method comprising.
A cosmetic composition for preservative, antioxidant, whitening and wrinkle improvement comprising the preservative composition of any one of claims 1 to 3.