JP2012201598A - Antioxidant and skin cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To find a material having high antioxidant action from among natural products having excellent safety, and to provide an antioxidant containing the material as an active ingredient.SOLUTION: The antioxidant is allowed to contain as an active ingredient, squeezed juice or an extract of a picked fruit of an apple cultivated without using intentionally a pesticide and a fertilizer containing a chemically synthesized material (excluding acetic acid and oil and fat) as an active ingredient. A skin cosmetic contains the blended squeezed juice or extract of a picked fruit of an apple cultivated without using intentionally a pesticide and a fertilizer containing a chemically synthesized material (excluding acetic acid and fat) as an active ingredient.

Description

  The present invention relates to an antioxidant and a skin cosmetic containing an apple juice or extract.

In recent years, active oxygen has attracted attention as a factor that particularly oxidizes biological components. Active oxygen is generated in the process of energy metabolism in living cells, and includes superoxide (ie, superoxide anion generated by one-electron reduction of oxygen molecules: .O ₂ ⁻ ), hydrogen peroxide (H ₂ O ₂ ), hydroxy Radical (.OH) and singlet oxygen ( ¹ O ₂ ). While these active oxygens are essential for the phagocytic sterilization mechanism, excessive production of active oxygen adversely affects the living body.

  For example, when excessive amounts of active oxygen are generated, they attack the biological molecules that make up membranes and tissues in the body, oxidize unsaturated fatty acids such as cholesterol and neutral fat, and increase the concentration of lipid peroxides. cause. The increase in lipid peroxide concentration in vivo is considered to be related not only to extensive membrane damage such as destruction of membrane structure and arteriosclerosis, but also to carcinogenesis and aging.

  In particular, since the skin is directly stimulated by environmental factors such as ultraviolet rays, the above-described superoxide is easily generated. Therefore, the increase in lipid peroxide concentration is thought to be one of the causes of skin aging such as increased wrinkles and decreased elasticity, pigmentation such as spots, dryness, rough skin, and skin inflammation. Yes. Therefore, it is considered that the above-mentioned skin aging symptoms and the like can be prevented, improved or treated by suppressing the formation of lipid peroxide.

  Therefore, an attempt has been made to obtain a substance having an antioxidative action from a natural product that is advantageous in terms of safety as compared with a chemically synthesized product. As one of such substances, there is an apple polyphenol derived from apples (patent documents 1 to 4) widely used for food.

Special table 2010-500016 gazette JP 2009-179570 A Japanese Patent Laid-Open No. 2002-47196 JP-A-7-285876

  However, since the above-mentioned apple polyphenol is derived from an apple cultivated by an agricultural method dependent on agricultural chemicals and fertilizers (hereinafter referred to as “conventional agricultural method”), it has not been able to meet consumer demand in terms of safety. There was a problem.

  The present invention has been made in view of the actual situation as described above, and finds a substance having a high antioxidant action from natural products excellent in safety, an antioxidant containing the substance as an active ingredient, and It aims at providing the skin cosmetics which mix | blended the said substance.

  In order to achieve the above-mentioned object, firstly, the present invention is a fruit of an apple fruit cultivated without intentionally using a pesticide and a fertilizer containing chemically synthesized substances (excluding acetic acid and fats and oils) as active ingredients. Provided is an antioxidant characterized by containing a juice or extract as an active ingredient (Invention 1).

  In the above invention (Invention 1), the fruit of apple harvest contains (1) a spray containing acetic acid and (2) a substance derived from a natural product as an active ingredient for controlling the disease or pest of the apple. It is preferably cultivated using at least one selected from the group consisting of a drug and (3) oils and fats, water-in-oil emulsions or oil-in-water emulsions (Invention 2).

  In the said invention (invention 1 and 2), it is preferable that the fruit fruit of the said apple is cultivated using the leguminous plant for the improvement of the soil which grows the said apple (invention 3).

  In the said invention (invention 1-3), it is preferable that the fruit fruit of the said apple is what was cultivated by the said soil whose microbial carbon amount in 100g of the soil which grows the said apple is 40.0 mg or more (invention). 4).

  2ndly, this invention mix | blended the juice extract or extract of the fruit of the fruit of the apple cultivated without intentionally using the agrochemical and fertilizer which use a chemically synthesized substance (except acetic acid and fats and oils) as an active ingredient A skin cosmetic is provided (Invention 5).

  ADVANTAGE OF THE INVENTION According to this invention, the antioxidant and skin cosmetics which are excellent in safety | security and have a high antioxidant action can be provided.

Hereinafter, an embodiment of the present invention will be described.
The antioxidant of this embodiment is a juice or extract of apple fruit that has been cultivated without intentionally using agricultural chemicals and fertilizers containing chemically synthesized substances (excluding acetic acid and fats and oils) as active ingredients. It is contained as an active ingredient. Moreover, the skin cosmetic of this embodiment mix | blends the said squeezed product or an extract.

  An apple (scientific name: Malus pumila), which is a raw material for the antioxidant and skin cosmetic of the present embodiment, is a deciduous tree belonging to the genus Rosaceae, and is cultivated in Japan, for example, in Aomori, Hokkaido and Nagano prefectures. Is done. The apple cultivar that can be used as a raw material in the present embodiment is not particularly limited, but Tsugaru, Obayashi, Jonagold, Hokuto, Mutsu, Chiaki, Benitama, Shinano Sweet, Hirosaki Fuji, Hack Nine, Shinano Gold, Sunlight, Sun Fuji, San Mutsu, Yellow Autumn, Kunimitsu, Shinko, New World, Tsugaru Gold, Akagi, Golden Delicious, Red Gold, Autumn, Venus, etc. Among these, Tsugaru, Obayashi, Hirosaki Fuji, and Huck Nine are varieties that are easy to grow apples as raw materials for the antioxidant and skin cosmetics of this embodiment.

  In this embodiment, the fruit of apple fruit is used as a raw material, not the mature fruit of apple. Here, in the present embodiment, the “fruit of fruit of apple” means a fruit that is thinned out for the purpose of increasing the size of the apple fruit to a desired range in the apple cultivation process, specifically, , Means apple fruit having a diameter of 6-2 cm, preferably 3-4 cm.

  The fruits of apples above are cultivated by a farming method that does not intentionally use agrochemicals and fertilizers containing chemically synthesized substances (excluding acetic acid and fats and oils) as active ingredients (hereinafter referred to as “pesticide-free and fertilizer-free farming methods”). It is a thing. In other words, in the present embodiment, it has been generally recognized that it is difficult to grow apples unless the conventional farming method is used, and the physiology of apples grown by the pesticide-free and fertilizer-free farming method. Against the backdrop of no report on activity, we found a substance derived from the fruit of the above apple fruit that has a high antioxidant action from natural products with excellent safety, and contained that substance in the antioxidant as an active ingredient Or the substance is blended in skin cosmetics.

  Here, in the present embodiment, “chemically synthesized substance” means a chemically synthesized substance. That is, even if the substance has the same chemical structure as the substance derived from the natural product, it is included in the “chemically synthesized substance” of the present embodiment as long as it is chemically synthesized.

  In the present embodiment, “agricultural chemicals” mean drugs such as insecticides and fungicides that are used to control diseases or pests that damage agricultural crops such as fungi, mites, and insects (hereinafter referred to as “pests”). To do. Many of these drugs contain chemically synthesized substances as active ingredients.

  Moreover, in this embodiment, "fertilizer" means the nutrient provided to a plant or land for the purpose of providing for plant nutrition. What is applied to plants or land for the purpose of soil modification is not included in the “fertilizer” in the present embodiment.

  In such an agrochemical-free fertilizer farming method, it is preferable to cultivate apple fruit by using a spray containing acetic acid in order to control pests. Acetic acid has an excellent bactericidal action, and can be applied to apples to kill bacteria that propagate around the leaves, thereby preventing black spot disease, spotted leaf disease, and the like. In addition, it is a suitable material for controlling pests because it is easy to adjust the dilution rate and can be prepared in large quantities at low cost.

  The method for preparing such a spraying agent is not particularly limited, but it is preferable to prepare vinegar by diluting vinegar with water and adding a spreading agent. In particular, vinegar is diluted 100 to 900 times with water and vinegar is used. On the other hand, it is more preferable to prepare by adding 0.2 to 0.6% by mass of a spreading agent. Thereby, since the viscosity of a spraying agent can be increased and a spraying agent becomes difficult to hang from a tree, the bactericidal action of acetic acid can be obtained efficiently.

  In the spray liquid adjusted using vinegar and a spreading agent as described above, the type of vinegar is not limited, and commercially available vinegar can be used. Commercially available vinegar contains 3 to 20% by mass of acetic acid, and additionally contains amino acids, alcohols and sugars. Moreover, it does not restrict | limit especially as water for diluting such vinegar, For example, tap water, well water, pure water, etc. can be used. Furthermore, the spreading agent added to the vinegar is not limited as long as it increases the viscosity of the vinegar, but for example, flour that absorbs moisture and produces sticky gluten can be used.

  When controlling pests using a spraying agent, it is preferable to spray the spraying agent by changing the dilution rate every predetermined day from mid-April until the fruit picking operation is performed. Thereby, a pest fruit can be efficiently obtained by controlling pests and insects. For example, by spraying a spraying agent obtained by diluting vinegar 200-300 times from mid-April, an environment in which bacteria are difficult to propagate can be created, and the spread of black spot disease can be prevented. In addition, by spreading a spraying agent obtained by diluting vinegar 800 times in mid-May, it is possible to prevent not only black spot disease but also spotted leaf defoliation while avoiding the effect on fruits that have started to fruit. Furthermore, after changing the dilution rate about every 10 days and spraying the spraying agent diluted with vinegar 300 to 600 times, the temperature of fruit rises in the rainy season and the temperature increases and the humidity increases. The sterilizing action of vinegar according to the above can be obtained, and the bacteria can be prevented from having resistance.

  After that, in order to prevent the black spot disease that can be activated by the long rain in autumn, the spraying of such spraying agents is repeated until the spraying agent in which vinegar is diluted 500 times is sprayed in the middle of September.

  In addition, in the agricultural chemical-free fertilizer farming method, it is preferable to cultivate apples using a drug containing a substance derived from a natural product as an active ingredient in order to control apple diseases. Examples of the drug containing a substance derived from a natural product as an active ingredient include a drug containing an extract from wasabi, tea, perilla, ume, garlic, lemon and the like as an active ingredient, and has a bactericidal property capable of controlling apple pests. Although it will not restrict | limit especially if it has, The coating agent which uses the extract from wasabi as an active ingredient is preferable among these. Wasabi has extremely high bactericidal properties. By applying such a coating agent to the bark of apples, it is possible to kill bacteria that propagate mainly in the bark and prevent rot and the like.

  Moreover, in the agricultural chemical-free fertilizer farming method, it is preferable to cultivate apples using fats and oils, water-in-oil emulsions or oil-in-water emulsions for the control of pests. Examples of fats and oils include edible oils and fats such as fatty oils and fats, industrial fats and oils, etc., as long as they have the property of attaching to wintering eggs of pests such as caterpillars and making them difficult to breathe by spraying on apples. Although it does not restrict | limit in particular, Tempura oil is preferable among these.

  Moreover, in the agricultural chemical-free fertilizer farming method, in order to control pests, it is preferable to use a water-in-oil emulsion or an oil-in-water emulsion in which a surfactant and water are added to the above oil and fat. It is preferable to use a water-in-oil emulsion or an oil-in-water emulsion to which water is added. As a result, compared with the case where surfactant and water are not added to the oil and fat, the oil component is dispersed in the water component to improve the handleability of the oil and fat, and the fluidity is improved and the oil and fat are easily dispersed. can do.

  Drugs and fats or oil and fat compositions containing such substances derived from natural products as active ingredients are extremely safer than pesticides containing chemically synthesized substances generally used in conventional farming methods as active ingredients.

  In addition, in the agricultural chemical-free fertilizer farming method, it is preferable to grow legumes around apples in order to adjust the nitrogen environment in the soil, especially at a position 0.5 to 3.5 m away from the trunk of the apple. It is preferred to grow legumes. Thereby, nitrogen can be replenished in soil at a position where there are many root hairs of apples that play a role of absorbing nutrients by utilizing nitrogen fixation performed by rhizobia dwelling in the roots of legumes. Such leguminous plant cultivation is intended to improve the soil in which apples are cultivated, and is not included in the fertilizer meant in this embodiment. In addition, the kind of leguminous plant is not particularly limited, but it is preferable to use soybeans because it is cheap and easy.

  When performing nitrogen supplementation to the soil using such legumes, it is easy and preferable to determine the deficiency and sufficiency of nitrogen in the soil based on the number of nodules of the legumes. For example, when the number of nodules of the leguminous plant is about 30 or more, the amount of nitrogen in the soil is insufficient, and it can be determined that it is preferable to continue cultivation of the leguminous plant. On the other hand, if the number of nodules of legumes is about 10 or less, the amount of nitrogen in the soil is sufficient, and the growth of legumes should be stopped to prevent excessive nitrogen in the soil. Can be determined to be preferable.

  In the pesticide-free and fertilizer-free farming method, the nitrogen component is replenished into the soil by the leguminous plants described above, so the amount of available nitrogen that can be absorbed by the plant in the soil provides fertilizer to supplement the nitrogen component. Compared to the above, the value is comparable or higher. Specifically, in a predetermined environment, when the amount of available nitrogen in 100 g of soil in the conventional farming method is about 10 mg, the amount of available nitrogen in 100 g of soil in the agricultural chemical-free fertilizer farming method is about 10 to 10 mg. 15 mg.

  In addition, in the agricultural chemical-free and fertilizer-free farming method, the circulation of microorganisms in the soil is not hindered by the agricultural chemicals, so the amount of microbial carbon, which indicates the amount of carbon in the soil, is compared with that in the conventional agricultural method using agricultural chemicals. And a large value. Specifically, in a predetermined environment, when the amount of microbial carbon in 100 g of soil in the conventional farming method is about 20 mg, the amount of microbial carbon in 100 g of soil in the agricultural chemical-free fertilizer farming method is about 40 mg or more. That is, in order to obtain the fruit of apple harvesting as a raw material of the present embodiment, the amount of microbial carbon in 100 g of soil growing apples is preferably 40.0 mg or more, and particularly the amount of microbial carbon is 42.0 mg or more. It is more preferable that

  Pesticide-free and fertilizer-free farming methods benefit from the benefits of microorganisms, etc., compared to conventional farming methods, and depending on the soil that is supplied with an appropriate amount of nitrogen without fertilizer, the antioxidant and skin cosmetics of this embodiment It is possible to cultivate fruit fruits of apples as raw materials.

  In the agricultural chemical-free fertilizer farming method described above, the above-mentioned method for controlling pests, that is, a method by spraying a spray containing acetic acid and a method by applying a drug containing a substance derived from a natural product as an active ingredient And the method of spraying oil or fat, water-in-oil emulsion, or oil-in-water emulsion may be carried out independently or in combination. If all of these are implemented, a wide variety of pests can be controlled. Moreover, the pest control method to implement can also be selected according to the environmental condition in which a pest may generate | occur | produce.

  In addition, in the agricultural chemical-free fertilizer farming method described above, pest control and nitrogen supplementation to the soil by legumes can be carried out independently. For example, when the amount of nitrogen in the soil is sufficient, while the growth of legumes is stopped, pest control is continued, so that the effects of pests can be prevented and the nitrogen in the soil can be prevented. Overloading can be prevented.

  Comparing the annual cultivation process of the pesticide-free and fertilizer-free farming method described above with that of the conventional farming method, it is basically different except for the control of the above-mentioned pests and nitrogen supply to the soil by legumes. The same as above. In other words, pesticide-free and fertilizer-free farming starts in February, pruning work around February, and using a legume plant around April, preparing a nitrogen environment in the soil and controlling pests by spraying sprays, etc. After the flowering, the fruit picking operation is started, and a mature apple fruit is obtained around September-October. In this annual cultivation process, fruit picking fruits of apples as raw materials for the antioxidant and skin cosmetics of this embodiment can be obtained by fruit picking work.

  It is possible to cultivate apples from seeds and seedlings in order to obtain the fruit of such apples, but if the environment for cultivating apples is established by conventional farming methods, the conventional farming method is changed to a pesticide-free and fertilizer-free farming method. It is preferable to migrate. In this case, if you secure a period of several years without pesticides, reduce the concentration of pesticide components remaining in the soil, and then shift to pesticide-free and pesticide-free farming methods, the fruit of apples is safer. Can be obtained. In addition, fruit-free fruits of apples that are pesticide-free and fertilizer-free can be obtained from the market. An example of the source is Kimura Konosha, Hirosaki City, Aomori Prefecture.

  The juice or extract used as the raw material of the present embodiment can be obtained from the fruit of apple fruit cultivated by the above-mentioned agricultural chemical-free fertilizer farming method. Here, in this embodiment, the “squeezed product” includes an apple mash, a squeezed solution obtained by applying physical force to the apple, a diluted or concentrated solution of the mash or squeezed solution, or These include any of the purees that are obtained by the backside treatment. The fruit juice extract of apple can be obtained, for example, by finely chopping the fruit of apple fruit used as a raw material and then subjecting it to a known pressing machine or the like. In order to prevent browning of the fruit juice of apple fruit, it is preferable to apply enzyme deactivation treatment by heating as described in the examples described later.

  Further, in the present embodiment, the “extract” includes an extract obtained by using apple fruit as an extraction raw material, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, or these Both of these crudely purified products and purified products are included. The extract from the fruit of picking apples can be obtained, for example, by drying the raw material for extraction, pulverizing it with a crusher, etc., and subjecting it to extraction with an extraction solvent.

  As the extraction solvent, it is preferable to use a polar solvent, and examples thereof include water and hydrophilic organic solvents. These are used alone or in combination of two or more at room temperature or a temperature below the boiling point of the solvent. It is preferable.

  Examples of water that can be used as the extraction solvent include pure water, tap water, well water, and the like, and water subjected to various treatments. Therefore, the water that can be used as the extraction solvent in this embodiment includes purified water, hot water, ion-exchanged water, phosphate buffered saline, and the like. Examples of the hydrophilic organic solvent that can be used as the extraction solvent include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol; lower aliphatic ketones such as acetone; and 2 to 5 carbon atoms such as 1,3-butylene glycol. And polyhydric alcohols.

  When using the liquid mixture of 2 or more types of polar solvents as an extraction solvent, the mixing ratio can be adjusted suitably. For example, when using a mixed liquid of water and a lower aliphatic alcohol, it is preferable to mix 1 to 100 parts by volume of a lower aliphatic alcohol with respect to 1 part by volume of water. When using a mixed liquid, it is preferable to mix 1 to 100 parts by volume of a lower aliphatic ketone with respect to 1 part by volume of water, and when using a mixed liquid of water and a polyhydric alcohol, water 1 It is preferable to mix 1 to 100 parts by volume of a polyhydric alcohol with respect to the volume part.

  The extraction treatment is not particularly limited as long as the soluble component contained in the extraction raw material can be eluted in the extraction solvent, and can be performed according to a conventional method. For example, the extraction raw material is immersed in an extraction solvent 5 to 15 times (mass ratio) of the extraction raw material, the soluble components are extracted at room temperature or under reflux, and then filtered to remove the extraction residue. You can get things.

  In order to obtain the juice or extract obtained as described above, a diluted or concentrated solution of the juice or extract, a dried product of the juice or extract, or a crude or purified product thereof. According to a conventional method, treatments such as dilution, concentration, drying, and purification may be performed. The obtained squeezed product or extract can be used as it is as an active ingredient of an antioxidant, but a concentrated solution or a dried product is easier to use.

  When the fruit juice extract or extract of apple has a specific odor, it can be purified for the purpose of decolorization, deodorization and the like within a range that does not cause a decrease in its physiological activity. When blended into skin cosmetics, etc., since it is not used in large quantities, there is no practical problem even if it is not purified.

  The fruit juice extract or extract of apples cultivated by the above agricultural chemical-free fertilizer farming method is excellent in safety, and, as will be apparent from Examples described later, lipid peroxide (lipid hydroxy oxide LOOH) ) Has an antioxidant action that can suppress the production of Therefore, such a fruit juice extract or extract of apples is suitable for containing as an active ingredient of an antioxidant.

  Said antioxidant may consist only of the squeezed product or extract of apple fruit, or may be a product of a squeezed product or extract of apple fruit.

  The fruit juice extract or extract of apple can be used by blending with other compositions (for example, skin cosmetics described later), skin absorption type preparations, ointments, haptics, patches. Can be used as a lotion, etc. It should be noted that the fruit juice extract or extract of apple fruit is in any dosage form such as powder, granule, liquid, milk, etc. according to a conventional method using a pharmaceutically acceptable carrier or any other auxiliary agent. It is also possible to formulate. In that case, as an auxiliary agent, an emulsifier, a suspending agent, an absorption accelerator, etc. can be used, for example. The antioxidant of the present embodiment can be used as an active ingredient by blending with other natural extracts having an antioxidant action, if necessary.

  The administration method of the antioxidant of the present embodiment generally includes transdermal administration, but a suitable method (for example, oral administration, transmucosal administration, etc.) may be appropriately selected according to the type of user. . The dosage of the antioxidant of the present embodiment may be appropriately increased or decreased depending on the age of the user, individual differences, administration method, administration period, and the like.

  Antioxidant of the present embodiment, through the antioxidant action of the fruit juice extract or extract of apple, aging of the skin such as wrinkle increase and reduced elasticity, pigmentation such as stains, dry, rough skin, Skin inflammation and the like can be prevented, ameliorated, or treated. However, the antioxidant of the present embodiment, in addition to these uses, prevents, improves, or improves all uses meaningful for exerting an antioxidative effect, for example, various disorders such as myocardial infarction, stroke, and cataract. It can also be used for therapeutic purposes.

  Moreover, the fruit juice extract or extract of apple fruit cultivated by the above-mentioned agricultural chemical-free fertilizer farming method is excellent in safety and has a high antioxidant effect. In particular, since it is excellent in the feeling of use or safety when applied to the skin, it is suitable for blending into skin cosmetics. In this case, the squeezed fruit juice extract or the extract can be directly blended into the skin cosmetic. Moreover, you may mix | blend the antioxidant formulated from the squeezed fruit juice extract or extract. Antioxidant action can be imparted to skin cosmetics by blending juice extract or extract of apple fruit, or an antioxidant formulated from these.

  There are no particular limitations on the type of skin cosmetic that can contain the fruit juice extract or extract of apples, and examples include ointments, creams, emulsions, lotions, packs, and foundations.

  When the juice extract or extract of apple fruit is blended into the skin cosmetic, the blending amount can be appropriately adjusted according to the type of skin cosmetic. A suitable blending ratio is 0.0005 to 0.45 mass% (in terms of solid content), and a particularly suitable blending ratio is 0.005 to 0.05 mass% (in terms of solid content). In addition, as will be apparent from the examples described later, the fruit juice extract or extract of apples has an antioxidant effect even at a lower concentration than the juice extract or extract of ripe apple fruit. Therefore, even if the blending rate in the skin cosmetics of the fruit juice extract or extract of apples is 0.0005 to 0.001% by mass (in terms of solid content), it is desired for the skin cosmetics. An action can be imparted.

  The above skin cosmetics are the main agents, auxiliaries or other components used in the production of normal skin cosmetics, for example, astringents, as long as they do not interfere with the antioxidant action of the fruit juice extract or extract of apples , Disinfectant / antibacterial agent, UV absorber, moisturizer, cell activator, anti-inflammatory / anti-allergic agent, antioxidant / active oxygen remover, oils / fats, waxes, hydrocarbons, fatty acids, alcohols, esters, Surfactants, fragrances and the like can be used in combination. By using together, it becomes a more general product, and the synergistic effect with the above-mentioned components used in combination may bring about an excellent use effect that is usually expected.

  The above-mentioned skin cosmetics are excellent in safety and have high antioxidant activity, such as skin aging such as wrinkles increase and elasticity decrease, pigmentation such as spots, dryness, rough skin, skin irritation, etc. Symptoms can be prevented, ameliorated or treated.

  The antioxidant and skin cosmetic of the present embodiment are preferably applied to humans, but may be applied to animals other than humans as long as each effect is exhibited. it can.

  EXAMPLES Hereinafter, although an Example and a comparative example are shown and this invention is demonstrated concretely, this invention is not restrict | limited to each following example at all.

[Example 1] Manufacture of fruit juice for fruit extraction Apples cultivated by an agricultural chemical-free fertilizer farming method, fruit fruit of diameter of Fuji (3 cm in diameter) were squeezed and filtered. The filtrate was heated at 80 ° C. for 30 minutes to obtain a fruit juice apple juice as a juice of apple fruit.

  1 mL of a coloring reagent (Wako Pure Chemical Industries, Foreign thiocult phenol reagent) that was added to a 15 mL centrifuge tube containing 4 mL of distilled water in advance and further diluted 5 times with distilled water was added to 50 μL of the obtained apple fruit juice. And stirred. Subsequently, 1 mL of a 10% (w / v) sodium carbonate (manufactured by Nacalai Tesque) solution was added, stirred, and allowed to stand in the dark for 1 hour. Thereafter, the absorbance at 760 nm was measured with a spectrophotometer (manufactured by Shimadzu Corporation, UVmini 1240), and the concentration of polyphenol contained in the fruit juice apple juice was calculated from the ratio to the measurement result of 2.5 mg / mL chlorogenic acid (manufactured by Aldrich). As a result, it was 2.04 mg / mL.

[Comparative Example 1] Manufacture of mature apple juice Mature apple juice as a squeezed product of mature apple fruit by the same method as in Example 1 except that mature apple fruits and Fuji species grown by conventional farming methods were used. Got. Moreover, it was 0.91 mg / mL when the polyphenol density | concentration of mature apple juice was computed by the method similar to Example 1. FIG.

[Comparative Example 2] Fruit Apple Polyphenol Solution 1.56 mg of a commercially available apple polyphenol powder (produced by Asahi Food and Healthcare, Applephenon SH) made from fruit fruits of apples cultivated by conventional farming methods is dissolved in 1 mL of distilled water. A fruit apple apple polyphenol solution was obtained. Moreover, it was 2.37 mg / mL when the polyphenol density | concentration of the fruit apple polyphenol solution was computed by the method similar to Example 1. FIG.

[Test Example 1] β-carotene fading test 1
A β-carotene fading test was carried out on Example 1 (fruit apple juice), Comparative Example 1 (mature apple fruit juice) and Comparative Example 2 (fruit apple polyphenol solution).

  10 mg of β-carotene (manufactured by Nacalai Tesque), 1.0 g of linoleic acid (manufactured by Nacalai Tesque) and 2.0 g of Tween 40 (manufactured by Kao Corporation, Rheodor TW-P120) are each added to 10 mL of chloroform (manufactured by Nacalai Tesque). Dissolved to obtain a β-carotene solution, a linoleic acid solution, and a Tween 40 solution. Then, 0.5 mL, 0.2 mL, and 1.0 mL of each solution were taken in a 300 mL Erlenmeyer flask, and chloroform was completely removed with nitrogen gas.

  Thereafter, 100 mL of distilled water was added and dissolved to obtain a linoleic acid / β-carotene aqueous solution. After adding 8 mL of 0.2 M phosphate buffer (pH 6.8) to 90 mL of an aqueous linoleic acid / β-carotene solution and stirring gently, 4.9 mL of the resulting mixture was dispensed into a 15 mL centrifuge tube, 0.1 mL of a test sample (see Table 1 for the polyphenol concentration in each sample of Example 1, Comparative Example 1 and Comparative Example 2) was added and mixed. Thereafter, the sample was quickly transferred to a constant temperature bath at 50 ° C., and the absorbance at 470 nm after 15 minutes and 45 minutes was measured with a spectrophotometer (manufactured by Shimadzu Corporation, UVmini 1240).

In addition, as a control sample of the test sample, the same operation and absorbance were obtained for an 80% ethanol solution (0.01 mg / mL) of a synthetic antioxidant BHA (manufactured by Nacalai Tesque, 3-t-Butyl-hydroxyanisole). Measurements were made. Furthermore, as Comparative Example 3, the same operation and measurement of absorbance were performed using an 80% ethanol solution (0.05 mg / mL) of a synthetic antioxidant BHA (manufactured by Nacalai Tesque, 3-t-Butyl-hydroxyanisole). (See Table 1 for BHA concentrations in Comparative Example 3 and the control sample).
From the results obtained as described above, the antioxidant activity value was calculated by the following formula.

Antioxidant activity value by β-carotene fading = (A−B) / (C−D)
In the formula, A represents “absorbance after 15 minutes of test sample”, B represents “absorbance after 45 minutes of test sample”, C represents “absorbance after 15 minutes of control sample”, D represents “absorbance after 45 minutes of control sample”.
The results are shown in Table 1.

  In Table 1, the antioxidant activity value is the ratio of the antioxidant activity to the control sample. The lower the value, the stronger the antioxidant activity, and the polyphenol concentration is a value converted to chlorogenic acid. As shown in Table 1, Example 1 (fruit extract apple juice) produced using apple fruit cultivated by pesticide-free and fertilizer-free farming as a raw material is produced using mature fruit cultivated by conventional farming. Compared to Comparative Examples 1 to 3 including Comparative Example 1 and Comparative Example 2 manufactured using fruit fruits of apples cultivated by the conventional farming method as raw materials, the antioxidant activity value was very low. That is, it was shown that Example 1 (fruit juice apple juice) has a high antioxidant action that can suppress the production of lipid peroxide (lipid hydroxyoxide LOOH).

[Test Example 2] β-carotene fading test 2
Example 1 (fruit juice apple juice) was obtained in the same manner as in Test Example 1, and then Example 1 (fruit apple juice) was diluted with distilled water, and the same test as in Test Example 1 was performed.
The results are shown in Table 2.

  In Table 2, as in Table 1, the antioxidant activity value is the ratio of the antioxidant activity to the control sample. The lower the value, the stronger the antioxidant activity, and the polyphenol concentration is converted to chlorogenic acid. It is the value. As shown in Table 2, Example 1 (fruit juice apple juice) produced using fruit from apples cultivated by agrochemical-free fertilizer farming as a raw material has its antioxidant activity value, that is, its antioxidant action is concentration-dependent. It was shown that even when the polyphenol concentration was 0.0102 mg / mL by dilution, it had a high antioxidant effect that could suppress the formation of lipid peroxide (lipid hydroxyoxide LOOH).

[Formulation Example 1]
According to the following composition, a skin cosmetic was produced by a conventional method.
Fruit juice extract of apples cultivated by pesticide-free and fertilizer-free farming method (Example 1) 2.00 g
Jojoba oil 4.00 g
1,3-butylene glycol 3.00 g
Arbutin 3.00g
Polyoxyethylene cetyl ether (20E.O.) 2.50g
2.00 g of olive oil
Squalane 2.00g
Cetanol 2.00g
Glyceryl monostearate 2.00g
Oleic acid polyoxyethylene sorbitan (20E.O.) 2.00g
Methyl paraoxybenzoate 0.15g
Stearyl glycyrrhizinate 0.10g
Twilight extract 0.10g
Dipotassium glycyrrhizinate 0.10g
Ginkgo biloba extract 0.10g
Conchiolin 0.10g
Oat extract 0.10g
Chamomile extract 0.10g
Fragrance 0.05g
Purified water remainder (total amount is 100 g)

  Antioxidants and skin cosmetics of the present invention prevent skin aging such as increased wrinkles and decreased elasticity, pigmentation such as spots, dryness, rough skin, skin inflammation and other symptoms caused by reactive oxygen species , Can greatly contribute to improvement or treatment.

Claims (5)

  It contains squeezed juice or extract of apple fruit that has been cultivated without intentionally using agrochemicals and fertilizers that contain chemically synthesized substances (excluding acetic acid and fats and oils) as active ingredients. Antioxidant. In order to control the fruit or fruit of the apple, the disease or pest of the apple,
A spray containing acetic acid,
It is cultivated using at least one selected from the group consisting of a drug containing a substance derived from a natural product as an active ingredient, and oils and fats, a water-in-oil emulsion, or an oil-in-water emulsion. The antioxidant according to claim 1.   The antioxidant according to claim 1 or 2, wherein the apple fruit is cultivated using legumes for the purpose of modifying the soil in which the apple is cultivated.   The fruit of apple picking is cultivated by the soil having a microbial carbon content of 40.0 mg or more in 100 g of the soil in which the apple is cultivated. Antioxidants as described in 1.   Skin makeup characterized by blending juice extract or extract of apple fruit cultivated without intentionally using pesticides and fertilizers that contain chemically synthesized substances (excluding acetic acid and fats and oils) as active ingredients Fee.