KR20230101373A - A beverage composition for preventing or improving hangover - Google Patents

Abstract

A beverage composition for preventing or improving a hangover that can quickly relieve a hangover by removing or neutralizing harmful substances such as alcohol absorbed into the body and acetaldehyde, a decomposition product, using mixed extracts of natural substances. , a beverage composition exhibiting a more improved effect is disclosed. The present invention provides a beverage composition for preventing or ameliorating a hangover comprising a complex extract of peony, dermis, baekchul and malt as an active ingredient.

Description

A beverage composition for preventing or improving hangover {A beverage composition for preventing or improving hangover}

The present invention relates to a beverage composition for preventing or alleviating a hangover, and more particularly, to a beverage composition for preventing or alleviating a hangover comprising a natural extract.

Consumption of alcohol is rapidly increasing to relieve stress of modern people, but symptoms such as nausea, vomiting, dizziness, thirst, lethargy, headache, and muscle pain due to acute alcoholic hangover are causing socio-economic damage due to reduced work efficiency. , chronic alcohol intake causes disorders such as pancreatitis, myocardial infarction, neuropathy, and tuberculosis, and furthermore, causes fatal damage to the structure and function of liver tissue.

Intermediate metabolites such as acetaldehyde, which are produced in the process of alcohol and oxidation, cause changes in various physiological actions, resulting in various metabolic diseases and alcoholic cirrhosis of the liver, and hangover symptoms appear after drinking alcohol. It is known that various hangover symptoms appear as the blood concentration of acetaldehyde, known as the main causative agent of hangover symptoms, increases.

Recently, several medicines have been developed to reduce or eliminate hangovers that occur after drinking, but due to their own toxicity or side effects, much attention is being paid to the development of safer natural product-derived health drinks. There is a continuing demand for natural foods that can more quickly reduce hangover symptoms such as drowsiness, headache, and dizziness.

[Prior patent literature]

- Korean Registered Patent No. 0392876 (2003.07.15.)

- Korean Patent Registration No. 1784870 (2017.09.28.)

The present invention is a beverage composition for preventing or alleviating a hangover, which can quickly relieve a hangover by removing or neutralizing harmful substances such as alcohol and acetaldehyde, a decomposition product, absorbed into the body using mixed extracts of natural substances. Compared to beverage products, it is intended to provide a beverage composition exhibiting a more improved effect.

In order to solve the above problems, the present invention provides a beverage composition for preventing or ameliorating a hangover comprising a complex extract of peony, dermis, baekchul and malt as an active ingredient.

In addition, the complex extract provides a beverage composition for preventing or improving a hangover, characterized in that it comprises 20 to 30% by weight of the peony, dermis, baekchul and malt, respectively.

In addition, the additive complex extract provides a beverage composition for preventing or improving a hangover, characterized in that the water extract.

In addition, the composition provides a beverage composition for preventing or improving a hangover, characterized in that the composition is orally administered in a dose of 50 to 300 mg / kg.

According to the present invention, it quickly relieves a hangover by removing or neutralizing harmful substances such as alcohol absorbed into the body and acetaldehyde, a decomposition product, including a mixed extract of peony, dermis, baekchul, and malt, and is a potent beverage product currently on the market. Contrast, it is possible to provide a beverage composition exhibiting a more improved effect.

1 is a diagram showing a schedule of drug administration, behavioral experiment, and blood collection in an experimental example of the present invention.
Figure 2 is a photograph showing the machine used in the Rota Rod (RR) experiment in the experimental example of the present invention.
Figure 3 is a graph showing the results of the behavioral experiment (Rota Rod) in the experimental example of the present invention.
Figure 4 is a graph showing the results of the behavioral experiment (LORR) in the experimental example of the present invention.
5 is a graph showing the results of measuring the concentrations of ethanol (A) and acetaldehyde (B) in blood in an experimental example of the present invention.

Hereinafter, the present invention will be described in detail through examples. Prior to this, the terms or words used in this specification and claims should not be construed as being limited to the usual or dictionary meaning, and the inventor appropriately uses the concept of the term in order to explain his/her invention in the best way. Based on the principle that it can be defined, it should be interpreted as meaning and concept consistent with the technical spirit of the present invention. Therefore, since the configurations of the embodiments described in this specification are merely the most preferred embodiments of the present invention and do not represent all of the technical spirit of the present invention, various equivalents and modifications that can replace them at the time of this application It should be understood that there may be

The inventors of the present invention are a conventional beverage composition using natural products, which can relieve hangover after drinking and more quickly reduce hangover symptoms such as thirst, drowsiness, headache, and dizziness. As a result of studying the effect of relieving hangover on complex extracts of natural substances, a specific combination of natural substances, that is, complex extracts including peony, dermis, baekchul and malt, showed significant effects in hangover relieving indicators compared to existing potent beverage products on the market. , It was confirmed that the hangover symptom improvement effect was better in the results of animal behavior experiments, and the present invention was reached.

Accordingly, the present invention discloses a beverage composition for preventing or alleviating a hangover comprising a complex extract of peony, dermis, baekchul and malt as an active ingredient.

The peony (芍藥, Paeonialactiflora) is a root of the Paeoniaceae family and is used as a treatment for abdominal pain, pain relief, antispasmodic, gynecological, high blood pressure and inflammation, and representative components include paeoniflorin and paeonol. ), and paeonin. Among the components of peony, paeoniflorin has been found to have an antioxidant effect, and effects such as anticancer activity and enhancement of immune function have been reported as pharmacological actions.

The dermis (陳皮, tangerine peel) is a well-known traditional herbal medicine, and the main active ingredient groups of the dermis include flavonoids, carotenoids, and phenethylamine alkaloids. These dermal components are known to exhibit pharmacological effects such as antihypertensive, antioxidant, antiaging, antibacterial and antiviral activities.

The baekchul (白朮) is a rhizome of Atractylodes japonica Koidzumi (Atractylodes macrocephala Koidzumi, Asteraceae Compositae), intact or with the main skin removed, and the function of the spleen is weak, resulting in news, boredom, and yellow face It is known to help excrete water when the whole body is swollen and indigestion due to water stasis, and it is good for thinning stool or diarrhea. has been

The malt (麥芽) is a medicine made by roasting and drying the sprouts of Poaceae ( Hordeum vulgare ), and malt enzyme amylase is produced and is called malt and is also used as a raw material for beer brewing. In oriental medicine, it was used to treat digestive disorders, indigestion and lack of milk secretion due to weakness in the stomach.

In the present invention, the hangover relief index is significantly improved in the beverage composition containing the composite extract containing all of the four natural substances compared to the existing dominant beverage product on the market (trade name 'Dawn 808'), and also in the results of animal behavior experiments. It was confirmed that a better hangover symptom improvement effect was shown, and it was confirmed that this effect was not implemented when any one of the natural products was not included.

In the present invention, it was found that there is a content ratio of each component to easily reproduce the remarkable effect when preparing a drink for preventing or improving hangover, and according to an embodiment of the present invention, the content ratio of each component is the total composite extract Based on the content, it is preferable that the peony, dermis, baekchul, and malt are each 20 to 30% by weight, and more preferably, the peony, dermis, baekchul, and malt content may be 23 to 27% by weight, respectively.

Preparation of the beverage composition for preventing or improving hangover according to the present invention may be performed through extraction, filtration and concentration steps. That is, it can be prepared by quantifying the peony, dermis, baekchul, and malt, extracting by introducing a solvent, filtering through a filter, and concentrating under reduced pressure using a concentrator,

The solvent is not particularly limited, but when water extraction is applied, it has been confirmed that it is the most preferable extraction method in consideration of improvement in indicators related to hangover relief.

In the case of water extraction, for example, 1 to 20 times, preferably 5 to 15 times, water is added to the total weight of the natural product, and hot water extraction is performed at 90 to 125 ° C, preferably 100 to 120 ° C for 1 to 10 hours. can be performed by

In addition, the water extraction may use various extraction methods such as reflux extraction, cold brew extraction, ultrasonic extraction, and compression extraction, but preferably, reflux extraction may be used.

Hereinafter, the present invention will be described in detail with examples and experimental examples.

Example

22 g each of peony, dermis, baekchul and malt (purchased from Nanum Pharmaceutical Co., Ltd.) was weighed and refluxed for 4 hours at 115 ° C with 1 liter of distilled water, filtered with a paper filter, and a rotary vacuum evaporator. It was concentrated under reduced pressure using 13 g (yield: 15%) of the powder obtained by freeze-drying the concentrated solution with a freeze dryer was stored in a cryogenic freezer (-80 ° C), and diluted in distilled water to the concentration required for the experiment.

On the other hand, as a positive control group, a commercially available potent hangover relieving beverage product (trade name 'Dawn 808') was purchased and used, and the extract obtained by concentrating one bottle (140 ml) under reduced pressure in the same way was 12 g.

Experimental example

[Experiment method]

(1) Laboratory animals and treatment

For rats, 6-week-old 180±10g Sprague-Dawely male rats (Hyochang Science) were purchased, acclimated to the laboratory for 1 week, stored in a plastic rat cage, and stored in an animal laboratory at 23±2℃, 50±10% relative humidity, and light. /dark cycle (12/12 hr), water and feed were allowed to be freely consumed, and used after adapting to the environment for a week before the start of the experiment.

They were divided into a normal group, an alcohol control group, a positive control group (Dawn 808), and a drug-administered group (complex extract 100 mg/kg, 200 mg/kg). Each group was divided into 8 normal groups, 8 control groups, 8 positive control groups, and 8 drug-treated groups, and 6-week-old rats were acclimatized for one week before the experiment. On the day of the experiment, the positive control drug (Dawn 808, 100 mg/kg) and the complex extract (100 mg/kg, 200 mg/kg) were orally administered, and the drug concentrations of all administration groups were measured and orally administered after oral administration of the drug. After 30 minutes, alcohol was orally administered at 30%/5ml/kg, and drugs were orally administered after 30 minutes. Behavior observation experiments were conducted at 30 minutes, 1, 2, 3, 5, and 7 hours at the time of alcohol administration, and 30%/5ml/kg alcohol was orally administered and blood samples were taken 1, 3, 5, and 7 hours later. did The drug administration, behavioral experiment and blood collection time schedules are shown in FIG. 1 .

(2) Behavioral experiment

First, for the Rota Rod (RR) experiment, they were trained for 1 minute at 8 rpm on the first day using a Rota Rod machine (see FIG. 2). After adapting to the Rota Rod by training at 16 rpm on the second day, 32 rpm on the third day, and 40 rpm for 2 days from the fourth day, behavior experiments were conducted. At 1, 2, and 3 hours after alcohol intake, behavioral experiments were conducted at 10 rpm, and at 5 and 7 hours, at 20 rpm. After a week of rest, RR and LORR (Loss of Righting Reflex) experiments were conducted, and after another week of rest, cardiac blood was collected hourly to measure ethanol and acetaldehyde in the blood.

The Rota Rod was adapted to the maximum speed (40 rpm) through training as an experimental method to evaluate the animal's limb movement coordination function and sense of balance. The experiment was conducted in a state in which all individuals perfectly adapted at 20 rpm, and the individuals maintaining the average were randomly separated into groups except for the non-adapted individuals.

In the LORR (Loss of Righting Reflex) experiment, to check the reaction of trying to straighten the head when the limbs are facing upwards with the back on the floor, the number of times of straightening when inverted three times for 30 seconds is 30 minutes after the administration of alcohol, 1 , 2, 3, 5 and 7 hours were measured at the time point.

(3) Measurement of ethanol and acetaldehyde in blood

After oral administration of 30%/5ml/kg alcohol, ether anesthesia was given after 1, 3, and 5 hours, and cardiac blood was drawn. At the last 7 hours, the abdomen was cut, blood was taken from the abdominal vena cava, and the blood was solidified for about 1 hour. Serum was obtained by centrifugation at 25° C. and 3,000 rpm for 20 minutes.

1) Measurement of ethanol in blood

Ethanol is oxidized by a liver enzyme called ADH (alcohol dehydrogenase) to produce acetaldehyde. In this process, NADH is produced with the help of NAD+ coenzyme, and the amount of the product is measured at absorbance of 340 nm. Mix 0.1 ml of blood with 3 ml of reaction mixture (potasium phosphate buffer pH 9 + NAD+ tablet), incubate at 20 ° C for 3 minutes, measure absorbance at 340 nm (A1), add 0.05 ml of ADH (alcohol dehydrogenase) and repeat After incubation at 20° C. for 5 minutes, absorbance is measured at 340 nm (A2), and blood ethanol is calculated according to Equation 1 below.

[Equation 1]

Concentration = 0.7259 /3.6 × ΔA

△A = Sample(A2-A1) - Blank(A2-A1)

2) Measurement of acetaldehyde in blood

Acetaldehyde is oxidized by a liver enzyme called acetaldehyde dehydrogenase (ALDH) to produce acetate. NADH is produced with the help of the NAD+ coenzyme, and the amount of the product is measured at absorbance at 340 nm. 0.2 ml of blood is mixed with 3 ml of reaction mixture (pottasium phosphate buffer pH 9 + NAD+ tablet 0.8 mg), incubated at 20 ° C for 3 minutes, and absorbance is measured at 340 nm (A1). 0.05 ml of ALDH is added here, incubated again at 20° C. for 5 minutes, and the absorbance is measured at 340 nm (A2) to calculate acetaldehyde in blood according to Equation 2 below.

[Equation 2]

Concentration = 0.7158 /3.6 × ΔA

△A = Sample(A2-A1) - Blank(A2-A1)

(4) Statistical processing

An Anova test was performed to determine whether there was significance between groups, and if there was significance between groups, whether or not the variance was equal was analyzed through an F test, and a student t-test was performed to determine the significance of p<0.05, p<0.01 and p<0.001. was analyzed.

[Experiment result]

(1) Behavioral experiment (Rota Rod)

Rats that had undergone Rota Rod adaptation for a week were randomly divided into each group, and behavioral experiments were conducted at 30 minutes, 1, 2, 3, 5, and 7 hours after alcohol intake, based on the time when all individuals had never fallen off. The exercise time of disabled rats was set to 0 seconds, and the exercise time for 120 seconds was measured, and the results are shown in FIG. 3 .

As a result of the experiment, all of the normal group (N) walked successfully at 10 and 20 rpm, but the alcohol control group (C) walked in a stretched state, and after 5 hours, all subjects became unable to walk.

The normal group (N) exercised for 120 seconds at all times, and it was confirmed that the exercise time was increased at all times in the positive control group (YM100) and drug-treated groups (JB100 and JB200) compared to the alcohol control group (C).

The exercise time of the positive control group (YM100) was similar to that of the alcohol control group (C). At 2 hours, all objects were awake, and there were some walking objects, but most of them were in a knockdown state where they could not exercise. The drug-administered group (JB100) was also in a similar state, but in the case of the drug-administered group (JB200), more than 4 individuals were maintained who could walk after 2 hours, and the number and exercise time able to exercise significantly compared to the alcohol control group (C). It was confirmed that this increase

In the case of the normal group (N), there was no rota rod immobility rat, but the number of immobility rats in the alcohol intake control group (C) was 2 by 3 hours, 4 by 5 hours, and 6 by 7 hours. .

At all times in the positive control group (YM100) and the drug-administered groups (JB100 and JB200), more or equal numbers of rats than the alcohol control group were able to exercise.

(2) Behavioral experiment (LORR)

As an experiment using the habit of trying to straighten the head for body equilibrium when the limbs are placed on the back and the limbs are facing the sky, the number of straight turns when turned over 3 times for 30 seconds was measured, and the results are shown in FIG. 4 .

As a result of the experiment, the normal group (N) quickly turned over all 3 times, and the alcohol control group (C) turned right over 2.1 times per hour on average, but they had no strength and the speed was very slow.

In the positive control group (YM100), although it was slow until 1 hour after alcohol intake, most of the subjects immediately returned all three times. After 3 hours, an average of 1.9 turns were successful, and the tendency to return was lower than that of the drug-treated group (JB100 and JB200), which was 2.2 times. In the case of the positive control group (YM100), especially after 7 hours, the number of individuals who could not recover at all in a state of weakness in the body increased.

After 3 hours, compared to the alcohol control group (C), the drug administration groups (JB100 and JB200) showed a significantly higher return frequency.

Therefore, it was found that the improvement in behavioral disorder was superior to the beverage composition according to the present invention than the currently marketed potent beverage product (YM100), and the effect was confirmed to be excellent in both 100 and 200 mg/kg doses.

(3) Measurement of ethanol and acetaldehyde in blood

After oral administration of 30%/5ml/kg alcohol, ether anesthesia was given after 1, 3, 5, and 7 hours, and cardiac blood was collected. After 7 hours, blood ethanol (A ) and acetaldehyde (B) concentration measurement results are shown in FIG.

As a result of analyzing the ethanol content in the blood, all experimental groups that consumed alcohol showed the highest blood alcohol concentration at 5 hours, and all showed a decreasing trend after 7 hours.

From 5 hours later, the blood alcohol concentration decreased significantly in the drug-administered groups (JB100 and JB200) compared to the alcohol-administered control group (C).

On the other hand, the positive control group (YM100) showed a significant decrease in blood alcohol content at 5 hours compared to the control group (C) who consumed only alcohol, and showed a tendency to decrease even at 7 hours, although there was no significance.

Acetaldehyde, an intermediate product of alcohol metabolism, is known to cause hangover symptoms, and it is known that the toxicity of alcohol in the liver is caused not only by alcohol itself but also by acetaldehyde produced during the process of alcohol metabolism in the liver. It is a strong toxic substance that is highly reactive and highly toxic compared to alcohol and is also known as the main cause of alcoholic liver damage. As a result of analyzing the blood content of acetaldehyde, an alcohol metabolite, an upward tendency was shown in all groups up to 5 hours, but in comparison between groups up to 5 hours, compared to the alcohol control group (C), the drug-administered groups (JB100 and JB200) The concentration decreased significantly from 3 hours to 5 hours. After 7 hours, the concentration decreased in all groups, and acetaldehyde was hardly measured, so there was no significance between groups.

Therefore, these results show that the complex extract according to the present invention can be usefully used for relieving hangover by promoting the activity of ADH and effectively reducing alcohol and acetaldehyde in the blood.

Preferred embodiments of the present invention described above have been disclosed to solve the technical problems, and those skilled in the art will be able to make various modifications, changes, additions, etc. within the spirit and scope of the present invention. , such modifications and changes should be regarded as belonging to the scope of the following claims.

Claims (4)

A beverage composition for preventing or improving a hangover, comprising a complex extract of peony, dermis, baekchul and malt as an active ingredient. According to claim 1,
The composite extract is a beverage composition for preventing or improving a hangover, characterized in that it comprises 20 to 30% by weight of the peony, dermis, baekchul and malt, respectively. According to claim 1,
Sanga complex extract is a beverage composition for preventing or improving a hangover, characterized in that the water extract. According to any one of claims 1 to 3,
The composition is a beverage composition for preventing or improving a hangover, characterized in that orally administered in a dose of 50 to 300 mg / kg.

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