CN110123952A - A kind of anti-alcoholism liver protecting compound preparation and its application - Google Patents
A kind of anti-alcoholism liver protecting compound preparation and its application Download PDFInfo
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Abstract
The invention discloses the preparations and application of a kind of relieving alcoholism and protecting liver compound extract, compound extract is main traditional Chinese medicine ingredients by the cogongrass cliff berry extract of pueraria lobata, green olive, hoveniae semoveniae semen, dendrobium candidum, cogongrass cliff berry, according to the basic principle of China's Traditional Chinese medical theory and treatment medication, screening, which is suited the medicine to the illness, in Chinese medicine abundant tests effect and a kind of formula of relieving alcoholism and protecting liver compound extract of proposition, pueraria lobata, hoveniae semoveniae semen and cogongrass cliff berry are combined for the first time, optimize the formula composition of relieving alcoholism and protecting liver；By the active measurement of enzyme activition of each extract to external alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) with screening as a result, then determining the formula of relieving alcoholism and protecting liver compound extract, it is ensured that the optimal proportion of formula；The compound extract is mixed with pharmaceutic adjuvant, oral preparation is made, it can be hard capsule, soft capsule, tablet, granule, powder, pill, tea bag, soft sweets, oral solution, any one in beverage, easy to use.
Description
Technical field
The present invention relates to a kind of anti-alcoholism liver protecting compound preparation and applications.
Background technique
Alcohol is a kind of most common drug and a kind of hepatotoxicant accepted extensively by society in world wide；In
The spirits culture of state is of long standing and well established, and wine is from, to today, existing more than 8,000 years history, over the past thousands of years, people are to wine at the beginning of invention
Love-hate relationship；Low alcohol consumption can cold dispelling promoting blood circulation and removing obstruction in channels, adjusting mood, elimination fatigue and tense situation；And intemperance then can
Multiple histoorgan lesion and damages such as cause including cardiovascular system, nervous system and digestive system.Compendium of Material Medica speech:
" crossing drink not save, murder in a moment "；Investigation is shown in the patient of long-term alcohol, and about 57.5% patient has fatty liver,
15% patient has cirrhosis；The tumour such as gastric cancer of some digestive systems, the cancer of the esophagus, liver cancer disease incidence rise also and in alcohol
Poison has close relationship；Alcoholism has threaten the health of people increasingly severely；Therefore, in order to reduce alcohol
The generation of poisoning, the demand for researching and developing related relieving alcoholism and protecting liver class product is extremely important and urgent, has in the existing formula that relieves the effect of alcohol
It is relieved the effect of alcohol material pueraria lobata with tradition a bit, hoveniae semoveniae semen is main composition, is relieved the effect of alcohol shield as patent No. 101658648B discloses one kind
Liver compound preparation and application are exactly with pueraria lobata, and hoveniae semoveniae semen is main composition, do not combine cogongrass cliff berry, and in the prior art
Relieving alcoholism and protecting liver formula is first determining formula, re-test activity, causes to determine in this way to be formulated and is not necessarily relieving alcoholism and protecting liver activity most
It is good.
Summary of the invention
Shortcoming present in view of the above technology, the present invention provide a kind of anti-alcoholism liver protecting compound preparation and application, energy
Pueraria lobata, hoveniae semoveniae semen and cogongrass cliff berry are enough combined, and again using the first enzyme activition activity of test alcohol dehydrogenase and acetaldehyde dehydrogenase
Determine that the mode of formula prepares the better effect so that relieving alcoholism and protecting liver.
To achieve the above object, the present invention provides a kind of anti-alcoholism liver protecting compound preparation, the relieving alcoholism and protecting liver compound system
Agent includes following component by weight percentage: pueraria lobata 10-30%, green olive 10-20%, hoveniae semoveniae semen 20-30%, dendrobium candidum
10-20%, cogongrass cliff berry extract 20-40%.
Wherein, the concrete component of the anti-alcoholism liver protecting compound preparation is as follows: pueraria lobata 20%, green olive 10%, hoveniae semoveniae semen
20%, dendrobium candidum 10%, cogongrass cliff berry extract 40%.
Wherein, the anti-alcoholism liver protecting compound preparation is oral agents anti-alcoholism liver protecting compound preparation.
Wherein, the anti-alcoholism liver protecting compound preparation is by the enzyme activition of external alcohol dehydrogenase and acetaldehyde dehydrogenase activity
Determining.
The present invention also provides a kind of preparation methods of anti-alcoholism liver protecting compound preparation, include the following steps:
Step A: relieving alcoholism and protecting liver medicine food plant folk prescription extract is prepared with water or ethyl alcohol；
Step B: to the enzyme activition screening active ingredients of alcohol dehydrogenase and acetaldehyde dehydrogenase outside each folk prescription extract extracting liq；
Step C: it is determined according to the result of alcohol dehydrogenase in step B) and the enzyme activition screening active ingredients of acetaldehyde dehydrogenase multiple
The formula composition of square extracting solution.
Wherein, the step A comprising the following specific steps
1), pueraria lobata, hoveniae semoveniae semen, dendrobium candidum, green olive, cogongrass cliff berry by the regulation of relevant criterion through after the assay was approved, it is standby
With；
2), fresh pueraria lobata is put into extractor as after relevant criterion ingredient, and each 5-6 times is measured 70% ethyl alcohol, impregnates 60 points
Clock extracts 2 times, and ethyl alcohol is recovered under reduced pressure in combined extract, and extracting solution is concentrated into no alcohol taste, centrifugation, and pueraria lobata extraction is obtained by filtration
Object；
3), hoveniae semoveniae semen is put into extractor as after relevant criterion ingredient, and each 5-6 times is measured 70% ethyl alcohol, impregnates 60 points
Clock extracts 2 times, and ethyl alcohol is recovered under reduced pressure in combined extract, and extracting solution is concentrated into no alcohol taste, centrifugation, and hoveniae semoveniae semen extraction is obtained by filtration
Object；
4), fresh dendrobium candidum is put into extractor as after relevant criterion ingredient, and each 5-6 times is measured water, impregnates 60 points
Clock extracts 2 times, combined extract, and extracting solution is concentrated into 1/10, and dendrobium candidum extract is obtained by filtration in centrifugation；
5), fresh green olive is put into extractor as after relevant criterion ingredient, and each 5-6 times is measured water, is impregnated 60 minutes,
It extracts 2 times, combined extract, extracting solution is concentrated into 1/10, and green olive extract is obtained by filtration in centrifugation；
6), cogongrass cliff berry is put into extractor as after relevant criterion ingredient, and each 5-6 times is measured 70% ethyl alcohol, impregnates 60 points
Clock extracts 2 times, and combined extract, extracting solution is concentrated into 1/10, by the D101 macroporous resin column pre-processed, first spends
Ion water elution, discards eluent, then with 10-30% ethanol elution, discards eluent, after with 50-70% ethanol elution, collects
Eluent recycles ethyl alcohol, dry, obtains cogongrass cliff berry.
Wherein, the step B comprising the following specific steps
1) pueraria lobata, hoveniae semoveniae semen, dendrobium candidum, green olive, cogongrass cliff berry extract prepare high, normal, basic three kinds of concentration, DMSO respectively
For cosolvent；
2) screen alcohol dehydrogenase (ADH) Activation Activity: the vitality test of alcohol dehydrogenase uses Wa Le-Huo Hefa；Side
Method is as follows: a certain amount of sodium pyrophosphate buffer solution, the ethanol solution of 11.5% (V:V), oxidized coenzyme I are separately added into test tube
(NAD+) and certain density folk prescription extracts liquor, is put in 25 DEG C of water-baths after mixing and keeps the temperature 10-15min, after heat preservation
A certain amount of alcohol dehydrogenase (ADH) is added in test tube immediately, measures extinction after shaking up at spectrophotometer 340nm wavelength immediately
Angle value (A340nm), then primary every 10-15s numeration, METHOD FOR CONTINUOUS DETERMINATION is mapped afterwards for a period of time, makees blank tune with distilled water
Zero, sample extracting solution is replaced as a control group with distilled water, measures the enzyme activity of control group alcohol dehydrogenase；
Activity ratio is calculated as follows:
Activity ratio=(enzyme activity test group-enzyme activity control group)/enzyme activity control group × 100%；
3), screen acetaldehyde dehydrogenase (ALDH) Activation Activity: the vitality test of acetaldehyde dehydrogenase uses Wa Le-Huo Hefa,
Method is as follows: acetaldehyde solution, the oxidized coenzyme I of a certain amount of sodium pyrophosphate buffer solution, 20.0% (V:V) are separately added into test tube
(NAD+) and certain density folk prescription extracts liquor, is put in 25 DEG C of water-baths after mixing and keeps the temperature 10-15min；After heat preservation
A certain amount of acetaldehyde dehydrogenase (ALDH) is added in test tube immediately, measures and inhales at spectrophotometer 340nm wavelength immediately after shaking up
Shading value (A340nm), then primary every 10-15s numeration, METHOD FOR CONTINUOUS DETERMINATION is mapped afterwards for a period of time；Make blank tune with distilled water
Zero, sample extracting solution is replaced as a control group with distilled water, measures the enzyme activity of control group acetaldehyde dehydrogenase；It is calculated as follows sharp
Motility rate:
Activity ratio=(enzyme activity test group-enzyme activity control group)/enzyme activity control group × 100%.
Wherein, the pueraria lobata, hoveniae semoveniae semen, dendrobium candidum, green olive, cogongrass cliff berry extract prepare described high, normal, basic three respectively
Kind concentration, high, normal, basic three kinds of concentration are respectively as follows: 1g/mL, 0.1g/mL, 0.01g/mL.
Wherein, step C comprising the following specific steps
1) each folk prescription extracting solution is matched according to enzyme activition vigor result, sets 5-10 group of formula, is configured to compound extract；
2) the enzyme activition activity of alcohol dehydrogenase and acetaldehyde dehydrogenase is tested the compound extract of each formula；
3) comprehensive reference enzyme activition active testing is as a result, determine three optimum proportioning prescriptions；
4) each folk prescription extract is mixed according to the proportion, be added auxiliary material to get.
In addition, the application of relieving alcoholism and protecting liver compound extract of the invention in the health care product and drug of effects of dispelling effects of alcohol and protecting liver,
The relieving alcoholism and protecting liver compound extract is obtained according to above-mentioned formula and method, which is characterized in that by the compound extract and medicine
It is mixed with auxiliary material, oral preparation is made, it can be hard capsule, soft capsule, tablet, granule, powder, pill, tea bag, soft
Sugar, oral solution, any one in beverage.
Pharmacological action of the present invention are as follows:
Pueraria lobata, first recorded in Shennong's Herbal, history tree is on the books, and the present is common crude drug, is arranged by health ministry
Enter " dual-purpose of drug and food " register；The effective component of pueraria lobata is mainly isoflavone compound, including Puerarin, daidzein
(daidzein), daidzin (daidzin) etc..Pueraria lobata as one of antialcoholic drugs most representative in traditional Chinese medicine,
With relieving alcoholism and preventing drunkenness, improve cardiovascular circulation, reduction myocardial oxygen consumption, reduces blood glucose, prevent hypertension and artery sclerosis, resist
Toxin for liver, anti-inflammatory, eliminating the phlegm is antipyretic, improves immunity of organisms, antibacterial, and the multiple pharmacological effects such as antiviral can effectively improve liver
Alcohol dehydrogenase (alcohol dehydrogenase, ADH) and acetaldehyde dehydrogenase (acetaldehyde dehydrogenase,
ALDH activity), antialcoholism action is related with the enhancing bioconversion of ethyl alcohol, from 3 absorption of ethyl alcohol, metabolism, excretion links
Antialcoholism action is played, antialcoholism action can be played to acute and chronic alcoholism.
Cogongrass cliff berry also known as vine tea, white tea etc., scientific name are ampelopsis grossdentata Ampelopsis grossed ntata
(Hand.-Mazz) W.T.Wang is the wild wooden defoliation liana of one kind of Vitaceae Ampelopsis, belongs to typical class
Tea plant is grown on ridges and peaks rows of mountains, dense vegetation, on fresh air, the soil is porous steep cliff fertile, in blinding cloud and mist, is a family
Boundary Mao Yanshan virgin forest rareness species；Civil that health protection tea is made in its young young stem and leaf, main chemical compositions have carbohydrate, amino
Acid, flavones etc..Wherein flavones content is higher, especially dihydromyricetin rich content；Modern medicine study shows that cogongrass cliff berry has
There is anti-inflammatory, antibacterial, cough-relieving, analgesia, liver protection, sober up and a series of effects such as antithrombotic, reducing blood lipid, anti-oxidant, inhibition tumour.
Hoveniae semoveniae semen is the seed of Rhamnaceae (Rham naceae) Hovenia (Hovenia Thunb) plant trifoliate orange Dulcis；Trifoliate orange Dulcis
Son has the effect of reducing fever and causing diuresis, relieving alcoholism, cures mainly the diseases such as wine poison, dysphoria with smothery sensation, thirsty, vomiting, difficulty in urination and defecation；It is civil to be known as " thousand glasss
The saying of not liquor-saturated hoveniae semoveniae semen ", also on the books in ancient medical book " hospital examines " " hoveniae semoveniae semen (cockspur), relieves the effect of alcohol, excessively flower of kudzuvine.It is modern
It is all afterwards to meet the middle wine person that gets sick from drinking too much wine, preferably use it." modern medicine study also turns out, hoveniae semoveniae semen can accelerating alcohol metabolism, reduce blood alcohol after drinking
Concentration enhances liver aldehyde dehydrogenase activity, reduces hepatic lipid peroxidation risk, reduces hepatic injury caused by ethyl alcohol etc..
Green Chinese olive tree Canarium album (Lour.) Raeusch. is the aiphyllium of Burseraceae Canarium, is China
A kind of economic fruit of southern some areas plantation, the dry fruit of maturation is Chinese olive, is that " Chinese Pharmacopoeia " records kind, has
Heat-clearing, relieving sore-throat are promoted the production of body fluid, the function of removing toxic substances, are suitable for abscess of throat, cough, polydipsia, fish and crab poisoning and wine poison etc., can be applied alone or
Compatible use.
Dendrobium candidum (Dendrobium officinale Kimura et Migo), which is that orchid family Dendrobium is perennial, grows nonparasitically upon another plant
Herbaceous plant also known as ribbed hedyotis herb, the even more top grade in dendrobium nobile medicinal material.It is used as medicine with its fresh or dry stem, there is " beneficial stomach life
The effect of saliva, nourishing Yin and clearing heat ", is known as " enriching yin sage product ".By iron sheet in Taoist school's medicine classical " Taoist Scriptures " before more than 1,000 years
Dendrobium nobile is classified as first of " Chinese nine big mesonas ", it is seen that its effect and status.
The beneficial effects of the present invention are: compared with prior art, the present invention combines pueraria lobata, hoveniae semoveniae semen and cogongrass cliff berry, together
When the effect of accomplishing relieving alcoholism and protecting liver；Meanwhile the activity by first testing and screening alcohol dehydrogenase and acetaldehyde dehydrogenase, then determine
The formula of compound preparation improves the reasonability of formula, the better effect of relieving alcoholism and protecting liver；A variety of shaped forms multidirectional can select.
Detailed description of the invention
Fig. 1 is alcohol dehydrogenase (ADH) vigor test chart；
Fig. 2 is acetaldehyde dehydrogenase (ADLH) vigor test chart.
Specific embodiment
Specific traditional Chinese medicinal components and preparation method and application based on foregoing invention content are come combined with specific embodiments below
Illustrate the embodiment and result of anti-alcoholism liver protecting compound preparation of the present invention.
Main become of wine is ethyl alcohol, two kinds in the enzyme system that the intracorporal catabolism of people mainly examines liver of alcohol
Enzyme: one is alcohol dehydrogenase, another kind is acetaldehyde dehydrogenase；Alcohol dehydrogenase can take off two hydrogen atoms in alcohol molecule
Fall, ethanol decomposition is made to become acetaldehyde, and acetaldehyde dehydrogenase can then take off two hydrogen atoms in acetaldehyde, and acetaldehyde is made to be converted into second
Acid is finally decomposed to carbon dioxide and water.
Anti-alcoholism liver protecting compound preparation of the invention be exactly by first determine anti-alcoholism liver protecting compound preparation alcohol dehydrogenase and
The influence of the enzymatic activity of acetaldehyde dehydrogenase determines the recipe ratio for the compound preparation that relieves the effect of alcohol, better effect later.
Cosolvent DMSO is a kind of liquid transparent, colourless, odorless, in slight bitter taste, referred to as " Dimethyl Sulfoxide ".
Embodiment one:
Fresh pueraria lobata: hoveniae semoveniae semen: fresh dendrobium candidum: fresh blueness olive: five kinds of extract formula rates of cogongrass cliff berry are 1:
Capsule is made in 3:1:1:4: taking kudzu root extract 2g, raisin tree seed extract 6g, dendrobium candidum extract 2g, green olive extract
Starch 60g is added in 2g, cogongrass cliff berry extract 8g, and beta cyclodextrin 50g, 95% alcohol granulation is dry, is made capsule 1000.
Embodiment 2, pueraria lobata: hoveniae semoveniae semen: fresh dendrobium candidum: fresh blueness olive: five kinds of extract formula rates of cogongrass cliff berry
Capsule is made for 2:2:1:1:4:
Take kudzu root extract 4g, raisin tree seed extract 4g, dendrobium candidum extract 2g, green olive extract 2g, cogongrass cliff berry
Starch 60g is added in general flavone 8g, and beta cyclodextrin 50g, 95% alcohol granulation is dry, is made capsule 1000.
Embodiment 3,
Pueraria lobata: hoveniae semoveniae semen: fresh dendrobium candidum: fresh blueness olive: five kinds of extract formula rates of cogongrass cliff berry are 2:2:1:
Capsule is made in 1:4:
Take kudzu root extract 4g, raisin tree seed extract 4g, dendrobium candidum extract 2g, green olive extract 2g, cogongrass cliff berry
Starch 60g is added in general flavone 8g, and beta cyclodextrin 50g, 95% alcohol granulation is dry, is made capsule 1000.
Correspondingly, three groups of comparative examples are set, are respectively as follows:
Comparative example 1
Fresh pueraria lobata: hoveniae semoveniae semen: fresh dendrobium candidum: fresh blueness olive: five kinds of extract formula rates of cogongrass cliff berry are 1:
Capsule is made in 2:2:2:3:
Take kudzu root extract 2g, raisin tree seed extract 4g, dendrobium candidum extract 4g, green olive extract 4g, cogongrass cliff berry
Starch 60g is added in extract 6g, and beta cyclodextrin 50g, 95% alcohol granulation is dry, is made capsule 1000.
Comparative example 2
Fresh pueraria lobata: hoveniae semoveniae semen: fresh dendrobium candidum: fresh blueness olive: five kinds of extract formula rates of cogongrass cliff berry are 1:
Capsule is made in 3:2:1:3:
Take kudzu root extract 2g, raisin tree seed extract 6g, dendrobium candidum extract 4g, green olive extract 2g, cogongrass cliff berry
Starch 60g is added in extract 6g, and beta cyclodextrin 50g, 95% alcohol granulation is dry, is made capsule 1000.
Comparative example 3
Fresh pueraria lobata: hoveniae semoveniae semen: fresh dendrobium candidum: fresh blueness olive: five kinds of extract formula rates of cogongrass cliff berry are 3:
Capsule is made in 2:2:1:2:
Take kudzu root extract 6g, raisin tree seed extract 4g, dendrobium candidum extract 4g, green olive extract 2g, cogongrass cliff berry
Starch 60g is added in extract 4g, and beta cyclodextrin 50g, 95% alcohol granulation is dry, is made capsule 1000.
Here is that embodiment 1-3 and comparative example 1-3 analyze the enzyme activition of alcohol dehydrogenase:
The activity of alcohol dehydrogenase (ADH) is measured after using Wa Le-Huo He (Valle&Hoch) method and slightly improveing, specifically
Steps are as follows:
Sodium pyrophosphate buffer solution 1.5mL, the 27mmol/L oxidized coenzyme I that pH value is 8.8 is separately added into measurement pipe
(NAD+) 1~3 product of ethanol solution 0.5mL, 0.1mg/mL embodiment and 0.1mg/mL comparative example of 1.0mL, 11.5% (v/v)
Solution (dissolves, it is cosolvent that a small amount of DMSO, which is added) 0.1mL with water after capsule decladding, after mixing, be put into 25 DEG C of water-bath and add
Lid incubates 5min；ADH (0.25U/mL) 0.1mL is added in measurement pipe after incubation immediately, is measured immediately with spectrophotometric after shaking up
Its fixed absorbance (A340nm) value, later primary every 10s reading, METHOD FOR CONTINUOUS DETERMINATION 5min is negated and initial linear portion is answered to be allocated as
Figure；The ethanol solution that 0.5mL11.5% (v/v) is replaced with 0.5m distilled water, makees reference zeroing, with the replacement of 0.1mL distilled water
0.1mL product solution measures the enzyme activity of control group A DH.
It is plotted against time with A340nm, negates and answer initial linear segment, △ A340nm/10s value is calculated, according to product
Molar extinction coefficient of the NADH at 340nm is 6.2, calculates the unit of activity of ADH；The vigor of ADH to generate NADH per minute
Nanomole number (nmol/min) indicate；Activity ratio is calculated as follows:
Activity ratio=(enzyme activity test group-enzyme activity control group)/enzyme activity control group × 100%
The result of generation is Fig. 1: visible to enhance second using relieving alcoholism and protecting liver product made from embodiment of the present invention method 1~3
Alcohol dehydrogenase (ADH) vigor effect is more apparent higher than comparative example 1~3 (different ratio), illustrates that Examples 1 to 3 formula is more reasonable.
Then, embodiment 1-3 and comparative example 1-3 analyze the enzyme activition of acetaldehyde dehydrogenase:
Method particularly includes: acetaldehyde dehydrogenase is measured using Wa Le-Huo He (Valle&Hoch) method and slightly after improvement
(ADLH) activity；Sodium pyrophosphate buffer solution 1.5mL, the 27mmol/L oxidized form that pH value is 9.5 is separately added into measurement pipe
Acetaldehyde solution 0.5mL, the 0.1mg/mL embodiment and 0.1mg/mL comparative example 1~3 of Coenzyme I (NAD+) 1.0mL, 20% (v/v)
Product solution (dissolves, it is cosolvent that a small amount of DMSO, which is added) 0.1mL with water after capsule decladding, after mixing, be put into 25 DEG C of water-bath
Middle capping incubates 5min；ALDH (0.25U/mL) 0.1mL is added in measurement pipe after incubation immediately, uses spectrophotometric after shaking up immediately
Meter measures its absorbance (A340nm) value, and later primary every 10s reading, METHOD FOR CONTINUOUS DETERMINATION 5min is negated and answered initial linear portion
It is allocated as figure.The acetaldehyde solution that 0.5mL20% (v/v) is replaced with 0.5mL distilled water, makees reference zeroing, with the replacement of 0.1mL distilled water
0.1mL product solution measures the enzyme activity of control group A DLH.
It is plotted against time with A340nm, negates and answer initial linear segment, △ A340nm/10s value is calculated, according to product
Molar extinction coefficient of the NADH at 340nm is 6.2, calculates the unit of activity of ADH；The vigor of ADH to generate NADH per minute
Nanomole number (nmol/min) indicate；Activity ratio is calculated as follows:
Activity ratio=(enzyme activity test group-enzyme activity control group)/enzyme activity control group × 100%
The result of generation is Fig. 2: as it can be seen that enhancing second using relieving alcoholism and protecting liver product made from embodiment of the present invention method 1~3
Aldehyde dehydrogenase (ADLH) vigor effect is apparently higher than comparative example 1~3 (different proportion), illustrates that Examples 1 to 3 formula is more reasonable.
Present invention has an advantage that
(1), pueraria lobata, hoveniae semoveniae semen and cogongrass cliff berry are combined, from pueraria lobata, hoveniae semoveniae semen, cogongrass cliff berry, green olive, in dendrobium candidum
It extracts with relieving alcoholism and protecting liver active material as gain-type fragrance, developing can prevent and alleviation bring of drinking is thirsty tired
Heat, the product of the symptoms such as dizziness vomiting；
(2), by the enzymatic activity first tested with screen alcohol dehydrogenase and acetaldehyde dehydrogenase, then matching for compound preparation is determined
Side, improves the reasonability of formula, the better effect of relieving alcoholism and protecting liver；
(3), compound extract preparation of the invention can become the product of diversified forms, be widely used, multidirectional selection.
The above content is a further detailed description of the present invention in conjunction with specific preferred embodiments, and it cannot be said that
Specific implementation of the invention is only limited to these instructions.For those of ordinary skill in the art to which the present invention belongs, exist
Several alternative or obvious variations are made under the premise of not departing from present inventive concept, and performance or use is identical, all should be considered as
It belongs to the scope of protection of the present invention.
Claims (10)
1. a kind of anti-alcoholism liver protecting compound preparation, which is characterized in that the anti-alcoholism liver protecting compound preparation includes such as by weight percentage
Lower component: pueraria lobata 10-30%, green olive 10-20%, hoveniae semoveniae semen 20-30%, dendrobium candidum 10-20%, cogongrass cliff berry extract
20-40%.
2. a kind of anti-alcoholism liver protecting compound preparation according to claim 1, which is characterized in that the anti-alcoholism liver protecting compound preparation
Concrete component it is as follows: pueraria lobata 20%, green olive 10%, hoveniae semoveniae semen 20%, dendrobium candidum 10%, cogongrass cliff berry extract 40%.
3. a kind of anti-alcoholism liver protecting compound preparation according to claim 1 or 2, which is characterized in that the relieving alcoholism and protecting liver is multiple
Square preparation is oral agents anti-alcoholism liver protecting compound preparation.
4. a kind of anti-alcoholism liver protecting compound preparation according to claim 1 or 2, which is characterized in that the relieving alcoholism and protecting liver is multiple
The weight percent of square preparation is determined by the enzyme activition activity of external alcohol dehydrogenase and acetaldehyde dehydrogenase.
5. a kind of preparation method of anti-alcoholism liver protecting compound preparation, which comprises the steps of:
Step A: relieving alcoholism and protecting liver medicine food plant folk prescription extract is prepared with water or ethyl alcohol；
Step B: to the enzyme activition of alcohol dehydrogenase and acetaldehyde dehydrogenase outside each folk prescription extract extracting liq prepared in step A
Screening active ingredients；
Step C: determine that compound mentions according to the result of alcohol dehydrogenase in step B) and the enzyme activition screening active ingredients of acetaldehyde dehydrogenase
Take the formula composition of liquid.
6. a kind of preparation method of anti-alcoholism liver protecting compound preparation according to claim 5, which is characterized in that the step A
Comprising the following specific steps
1), pueraria lobata, hoveniae semoveniae semen, dendrobium candidum, green olive, cogongrass cliff berry by the regulation of relevant criterion through after the assay was approved, it is spare；
2), fresh pueraria lobata is put into extractor as after relevant criterion ingredient, and each 5-6 times is measured 70% ethyl alcohol, is impregnated 60 minutes,
It extracts 2 times, ethyl alcohol is recovered under reduced pressure in combined extract, and extracting solution is concentrated into no alcohol taste, and kudzu root extract is obtained by filtration in centrifugation；
3), hoveniae semoveniae semen is put into extractor as after relevant criterion ingredient, and each 5-6 times is measured 70% ethyl alcohol, is impregnated 60 minutes, is mentioned
It takes 2 times, combined extract, ethyl alcohol is recovered under reduced pressure, extracting solution is concentrated into no alcohol taste, and raisin tree seed extract is obtained by filtration in centrifugation；
4), fresh dendrobium candidum is put into extractor as after relevant criterion ingredient, and each 5-6 times is measured water, is impregnated 60 minutes, is mentioned
It takes 2 times, combined extract, extracting solution is concentrated into 1/10, and dendrobium candidum extract is obtained by filtration in centrifugation；
5), fresh green olive is put into extractor as after relevant criterion ingredient, and each 5-6 times is measured water, is impregnated 60 minutes, extracts 2
Time, combined extract, extracting solution is concentrated into 1/10, and green olive extract is obtained by filtration in centrifugation；
6), cogongrass cliff berry is put into extractor as after relevant criterion ingredient, and each 5-6 times is measured 70% ethyl alcohol, is impregnated 60 minutes, is mentioned
It takes 2 times, combined extract, extracting solution is concentrated into 1/10, by the D101 macroporous resin column pre-processed, first uses deionized water
Elution, discards eluent, then uses 10-30% ethanol elution, discards eluent, after with 50-70% ethanol elution, collects and elutes
Liquid recycles ethyl alcohol, dry, obtains cogongrass cliff berry extract.
7. a kind of preparation method of anti-alcoholism liver protecting compound preparation according to claim 5, which is characterized in that the step B
Comprising the following specific steps
1) pueraria lobata, hoveniae semoveniae semen, dendrobium candidum, green olive, cogongrass cliff berry extract prepare high, normal, basic three kinds of concentration respectively, and DMSO is to help
Solvent；
2) screen alcohol dehydrogenase (ADH) Activation Activity: the vitality test of alcohol dehydrogenase uses Wa Le-Huo Hefa, and method is such as
Under: a certain amount of sodium pyrophosphate buffer solution, the ethanol solution of 11.5% (V:V), oxidized coenzyme I (NAD+) are separately added into test tube
Liquor is extracted with certain density folk prescription, is put after mixing in 25 DEG C of water-baths and keeps the temperature 10-15min, the test tube after heat preservation is vertical
A certain amount of alcohol dehydrogenase (ADH) is added, measures absorbance value after shaking up at spectrophotometer 340nm wavelength immediately
(A340nm), then primary every 10-15s numeration, METHOD FOR CONTINUOUS DETERMINATION is mapped afterwards for a period of time, makees blank zeroing with distilled water, with
Distilled water replaces sample extracting solution as a control group, measures the enzyme activity of control group alcohol dehydrogenase；Activity ratio is calculated as follows:
Activity ratio=(enzyme activity test group-enzyme activity control group)/enzyme activity control group × 100%；
3) screen acetaldehyde dehydrogenase (ALDH) Activation Activity: the vitality test of acetaldehyde dehydrogenase uses Wa Le-Huo Hefa, and method is such as
Under: a certain amount of sodium pyrophosphate buffer solution, the acetaldehyde solution of 20.0% (V:V), oxidized coenzyme I (NAD+) are separately added into test tube
Liquor is extracted with certain density folk prescription, is put after mixing in 25 DEG C of water-baths and keeps the temperature 10-15min；Test tube after heat preservation is vertical
A certain amount of acetaldehyde dehydrogenase (ALDH) is added, measures absorbance value after shaking up at spectrophotometer 340nm wavelength immediately
(A340nm), then primary every 10-15s numeration, METHOD FOR CONTINUOUS DETERMINATION is mapped afterwards for a period of time；Make blank zeroing with distilled water, with
Distilled water replaces sample extracting solution as a control group, measures the enzyme activity of control group acetaldehyde dehydrogenase；Activity ratio is calculated as follows:
Activity ratio=(enzyme activity test group-enzyme activity control group)/enzyme activity control group × 100%.
8. according to a kind of preparation method of anti-alcoholism liver protecting compound preparation as claimed in claim 6, which is characterized in that the pueraria lobata, trifoliate orange
Dulcis, dendrobium candidum, green olive, cogongrass cliff berry extract prepare high, normal, basic three kinds of concentration respectively, and described high, normal, basic three kinds dense
Degree is respectively as follows: 1g/mL, 0.1g/mL, 0.01g/mL.
9. a kind of preparation method of anti-alcoholism liver protecting compound preparation described in claim 5, which is characterized in that the step C includes
Step in detail below:
1) each folk prescription extracting solution is matched according to enzyme activition vigor result, sets 5-10 group of formula, is configured to compound extract；
2) the enzyme activition activity of alcohol dehydrogenase and acetaldehyde dehydrogenase is tested the compound extract of each formula；
3) comprehensive reference enzyme activition active testing is as a result, determine three optimum proportioning prescriptions；
4) each folk prescription extract is mixed according to the proportion, be added auxiliary material to get.
10. application of the relieving alcoholism and protecting liver compound extract in the health care product and drug of effects of dispelling effects of alcohol and protecting liver, the relieving alcoholism and protecting liver compound
Extract is obtained according to the described in any item formulas of the claims 1-8 and method, which is characterized in that extracts the compound
Object is mixed with pharmaceutic adjuvant, and oral preparation is made, it can be hard capsule, soft capsule, tablet, granule, powder, pill, bag
It makes tea, any one in soft sweets, oral solution, beverage.
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| CN113171397A (en) * | 2021-06-07 | 2021-07-27 | 劲牌有限公司 | Hangover alleviating traditional Chinese medicine composition for activating acetaldehyde dehydrogenase and preparation method thereof |
| CN114631632A (en) * | 2022-04-20 | 2022-06-17 | 广东省刺梨产业研究院 | Rosa roxburghii Tratt composition for protecting liver and alleviating hangover as well as preparation and application thereof |
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