CN103203008A - Pharmaceutical composition for sobering up and preparation method thereof - Google Patents
Pharmaceutical composition for sobering up and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a pharmaceutical composition for sobering up and a preparation method thereof. The pharmaceutical composition comprises the following components in parts by weight: 55-65 parts of orange peel, 25-35 parts of tea leaf, 65-75 parts of kudzuvine root, 55-65 parts of raisin tree seed, 75-85 parts of lalang grass rhizome, 65-75 parts of common lophatherum herb, 45-55 parts of liquorice root, 95-105 parts of raw olive fruit, 95-105 parts of raw Chinese wampee fruit, 0.6-1.0 part of L-cysteine and 4.0-5.0 parts of corn peptide. The pharmaceutical composition disclosed by the invention is finely mixed by an inventor by adopting pure natural medicines or components extracted from natural plants. The pharmaceutical composition disclosed by the invention has the effect of protecting the liver, and can thoroughly relieve nausea, vomiting, headache and other symptoms after a person is drunk.
Description
Technical field
The invention belongs to field of pharmaceutical preparations, be specifically related to a kind of be used to pharmaceutical composition that relieves the effect of alcohol and preparation method thereof.
Background technology
In China and even global, the production of wine has long history with consumption, has formed huge industry and various nationalities' spirits culture.With the progress of expanding economy and society, the effect of wine in economic life becomes increasingly conspicuous.Wine is a double-edged sword, and a spot of drinking is the friend of health, and insobriety is the arch-criminal.The excessive consumption of alcohol meeting causes the acute or chronic poisoning of ethanol, but can have wine in the life, and the whirl of society more can not keep from drink.
World Health Organization (WHO) points out: alcoholism is first public hazards in world today's scope.Drunk reality is acute alcoholism, and after ethanol (ethanol) entered human body, 20% absorbed immediately under one's belt, and all the other 80% are absorbed by duodenum and jejunum.That drinks 60% absorbed in first hour, and two hours absorbtivity is up to more than 90%, all was absorbed in three hours.After human body was taken in ethanol, ethanol can enter cell and be oxidized to acetaldehyde.
The orthobiosis that the alcoholism that long-term excessive or disposable a large amount of absorption ethanol causes and complication have had a strong impact on people, according to statistics, in American-European countries, alcoholism is to rank the cardiovascular disease tumor to account for deputy public health problem afterwards, the general crowd's of the U.S. lifetime prevalence is up to 50%, and has that to surpass 70% inpatient be because the acute poisoning that abuse of alcohol causes is caused.China is the production and consumption big country of ethanol, and therefore the alcoholism patient enormous amount that causes by drinking develops the medicine of effective preventing drunkenness relieving alcoholic intoxication and hepatoprotective and the generally attention that health food is subjected to medicine and food service industry.
Alcoholism is commonly called as drunk, once drinks a large amount of inebriants and can produce excitement back inhibitory action earlier to the central nervous system, and severe intoxication can make breathing, heart beating suppress and death.The daily all kinds of wine of drinking all contain not commensurability ethanol (ethanol), and it is more high that beverage contains amount of alcohol, absorb more fast, more easy to be fascinating.
Ethanol content in the common inebriant is as follows: medicated beer 3%～5%; Yellow wine 16%～20%; Fruit wine 16%～48%, wine 18%～23%; Chinese liquor 35%～65%, low alcohol white spirit newly developed in recent years, ethanol content is 24%～38%.
Neither one really has the product of relieving alcoholic intoxication or antialcoholism function in the market, and the Chinese medicine detoxicating that relieves the effect of alcohol has distinctive feature, actively develops Chinese medicine and is used for relieving the effect of alcohol, and has become new problem.
Summary of the invention
Based on this, be necessary to provide a kind of be used to pharmaceutical composition that relieves the effect of alcohol and preparation method thereof.
A kind of be used to the pharmaceutical composition that relieves the effect of alcohol, by weight, comprise following component:
Pericarpium Citri tangerinae 55-65 part, Folium Camelliae sinensis 25-35 part, Radix Puerariae 65-75 part, Semen Hoveniae (Fructus Hoveniae) 55-65 part, Rhizoma Imperatae 75-85 part, Herba Lophatheri 65-75 part, Radix Glycyrrhizae 45-55 part, living Fructus Canarii albi fruit 95-105 part, SHENGHUANG follicarpium 95-105 part, L-cysteine 0.6-1.0 part, Semen Maydis polypeptide 4.0-5.0 part.
Among some embodiment, described pharmaceutical composition comprises following component therein:
60 parts of Pericarpium Citri tangerinaes, 30 parts of Folium Camelliae sinensis, 70 parts of Radix Puerariaes, 60 parts of Semen Hoveniae (Fructus Hoveniae)s, 80 parts of Rhizoma Imperataes, 70 parts of Herba Lophatheris, 50 parts in Radix Glycyrrhizae, 100 parts of living Fructus Canarii albi fruits, 100 parts of SHENGHUANG follicarpiums, 0.8 part of L-cysteine, 4.5 parts of Semen Maydis polypeptides.
The components description of pharmaceutical composition of the present invention is as follows:
Pericarpium Citri tangerinae is the mature peel of rutaceae orange and variety thereof, is called Pericarpium Citri Reticulatae again after putting for a long time, and 10 during to the December fruit maturation, takes fruit, strips peel, dries in the shade or aeration-drying.Wide Pericarpium Citri tangerinae is slit into 3 to 4 lobes more when stripping.Pericarpium Citri tangerinae medical material branch " Pericarpium Citri Reticulatae " and " Pericarpium citri reticuatae chachiensis ", having regulates the flow of vital energy transfers, and the drying dampness to eliminate phlegm effect can be used for treating stagnation of QI in spleen and stomach, distension and fullness in the abdomen, vomiting, or effect such as uncomfortable in chest due to the turbid damp obstructing in middle-JIAO, indigestion and loss of appetite, loose stool.
Semen Hoveniae (Fructus Hoveniae) (Semen Hoveniae), another name Semen Hoveniae (Fructus Hoveniae), chicken feet pears, chicken square, sweet midnight, manna, tree honey etc. are the seed of Rhamnaceae (Rhamnaceae) plant Semen Hoveniae (Fructus Hoveniae) (Hovenia duleis Thunb.) drying and ripening.The property flat, sweet in the mouth acid, nontoxic, the effect of clearing away heat and promoting diuresis, relieving alcoholic intoxication poison is arranged, cure mainly diseases such as drunk, dysphoria with smothery sensation, thirsty, vomiting, lower abdomen contracture, difficulty in urination and defecation.Studies show that Semen Hoveniae (Fructus Hoveniae) contains multiple compositions such as saponin, alkaloid, flavonoid, have relieving alcoholic intoxication, protect the liver, pharmacological action such as anti peroxidation of lipid, inhibition nervus centralis.
Radix Puerariae (Radix Puerariae Lobatae), another name Radix puerariae, Pachyrhizua angulatus, Radix Puerariae, Pueraria lobata, Pueraria montana (Lour.) Merr. etc., dry root for the perennial defoliation liana of pulse family (Leguminosae sp.) Pueraria lobota (Pueraria loba ta (Willd.) Ohwi), cool in nature, sweet in the mouth, suffering, supplement to the Herbal are called its " relieving alcoholic intoxication poison ".Modern study shows that Radix Puerariae contains the necessary aminoacid of multiple human body, vitamin and flavone etc., has multiple pharmacological effect such as treatment cardiovascular and cerebrovascular disease, prevention of arterial sclerosis, antioxidation, facilitating alcohol metabolism and protecting liver.
Rhizoma Imperatae is the rhizome of grass perennial herb Herba Imperatae (Imperata cylindrica), another name: Rhizoma Imperatae, manage, eat root, blue root.Two seasons of spring and autumn excavate and dry, and remove fibrous root and membranous sheath, and cutting is given birth to and used or parch to black usefulness.Cure mainly: cooling blood for hemostasis, heat-clearing and toxic substances removing.Be used for to spit blood, hematuria, pyretic stranguria, edema, jaundice, dysuria, calentura excessive thirst, the gastric heat sound of vomiting of vomitting is coughed.
Herba Lophatheri, grass family Herba Lophatheri platymiscium.Cure mainly disease heat in the heart, cough with dyspnea.Haematemesis, pyretic toxicity wind, only quench one's thirst, press the borax poison.Fanatical unhappiness is controlled in expectorant.Apoplectic aphasia in silence, the pain wind syndrome of head, spasmolytic is throbbed with fear, pestilence fan is vexed, pregnant woman's head revolves and falls down to the ground, children's hangs sore throat, dysphoria with smothery sensation in frightened carbuncle sky.
Radix Glycyrrhizae (Radix Glycyrrhizae) is a kind of tonification Chinese herbal medicine.Medicinal part is root and rhizome, feeble QI, and it is sweet and special to distinguish the flavor of.Function cures mainly heat-clearing and toxic substances removing, expelling phlegm for arresting cough, gastral cavity abdomen etc.
Fructus Canarii albi has another name called Fructus Canarii, can gain the name for eating raw when cause and effect still is dark green in fact.Fructus Canarii albi really is rich in calcareous and compositions such as vitamin C, flavone, the big benefit of Yu Renyou.Eat, boil drink raw, all can separate drunkly, separate Fugu ocellatus ichthyotoxin.Chew juice and swallow, control fishbone fishbone and all fish Eriocheir sinensis poison.The effect of promoting the production of body fluid to quench thirst is arranged again, control throat pain.In the present invention, use is the fruit that gives birth to of Fructus Canarii albi.
Clausena lansium (Lour.) Skeels (Clausena lansium), fruit is rich in sugar, organic acid, pectin, vitamin C, volatile oil, flavonoid glycoside etc.Clausena lansium (Lour.) Skeels have eliminate distension and fullness of the chest and abdomen, promote the production of body fluid, quench the thirst, the effect of pleasant, antitussive; The Clausena lansium (Lour.) Skeels that bitterness is heavier has better effect.Clausena lansium (Lour.) Skeels also has higher medical value, and root, leaf, fruit and seed etc. all can be used as medicine, and has effects such as promoting digestion and invigorating the stomach, regulating qi-flowing for strengthening spleen, promoting the circulation of QI to relieve pain, and custom has saying of " hungry food Fructus Litchi, the Clausena lansium (Lour.) Skeels of being satiated with food ".
L-cysteine (L-Cysteine) is white crystals or crystalline powder, and is water-soluble, and little have smellyly, is insoluble in ethanol, is insoluble to organic solvents such as ether.240 ℃ of fusing points, monoclinic system.Cysteine is one of sulfur-containing amino acid, belongs to non essential amino acid.Has chelation etc. in vivo, so a lot of poisonous substances such as formaldehyde, acetaldehyde, chloroform, carbon tetrachloride, lead, cadmium, chloromethane hydrargyrum, lipid peroxidation, PCB, tetrodotoxin, TTX, ethanol etc. are had effective Detoxication.
Semen Maydis polypeptide is the protein that extracts from wholefood-Semen Maydis, again the micromolecule polypeptide material of the directed enzyme action of process and specific little peptide isolation technics acquisition.Semen Maydis polypeptide can suppress stomach to the absorption of ethanol, increases the activity of the interior ethanol dehydrogenase of body and aldehyde dehydrogenase, promotes ethanol metabolism and discharge in vivo.
Above component 9 flavor Chinese herbal medicine are through infusion and remove impurity, with L-cysteine and the rational compatibility of Semen Maydis polypeptide process, obtain having the product of obvious anti-alcohol function.
The present invention also provides the preparation method of aforementioned pharmaceutical compositions.
Described preparation of drug combination method may further comprise the steps:
(1) with the component mixing of the 9 kinds of pharmaceutical compositions of the present invention except L-cysteine and Semen Maydis polypeptide, add its gross weight 8-12 water boil doubly and extract 2-3 time, each 1.5-2hr is chilled to room temperature filtering precipitation and obtains filtrate;
(2) above-mentioned filtrate is gone up the AB-8 macroporous adsorbent resin, with 4-6 times of resin column volume washing, with the 85-95% ethanol elution of 4-6 times of volume, concentrate eluant to a three/volume obtains extractum then;
(3) above-mentioned extractum spray drying is obtained dry powder; Add L-cysteine, Semen Maydis polypeptide, namely get pharmaceutical composition of the present invention.
It is fine powder that pharmaceutical composition is pulverized, and sieves, and makes corresponding dosage form according to technology and the adjuvant requirement of various dosage forms.Can be made into tablet, granule, capsule, pill or powder.
Pharmaceutical composition of the present invention be the inventor adopt pure natural medical or the component that obtained by extracted form natural plant meticulously allotment form.The function that relieving alcoholic intoxication relieves the effect of alcohol is played in 11 kinds of medicine combineds effect altogether.Copy the alcoholism mouse model through heavy dose and long-term ethanol are irritated stomach, confirming that pharmaceutical composition of the present invention has relieves the effect of alcohol, effects such as Soboring-up liver-protecting, and its antialcoholism action mechanism carried out preliminary discussion.Simple, the easy row of preparation of drug combination method of the present invention, production cost is low.
The specific embodiment
Describe the present invention in detail below in conjunction with specific embodiment.
Employed raw material all derives from commercially available in following examples.
The tablet of embodiment 1 pharmaceutical composition of the present invention
The component that comprises following weight portion:
Pericarpium Citri tangerinae 60kg, Folium Camelliae sinensis 30kg, Radix Puerariae 70kg, Semen Hoveniae (Fructus Hoveniae) 60kg, Rhizoma Imperatae 80kg, Herba Lophatheri 70kg, Radix Glycyrrhizae 50kg, Fructus Canarii albi fruit (life) 100kg, Fructus clausenae lansii (life) 100kg, L-cysteine 0.8kg, Semen Maydis polypeptide 4.5kg.
Preparation method is:
(1) will be except the said components mixing of L-cysteine and Semen Maydis polypeptide, the rustless steel that adds 2 tons extracts in the pot, and the water boil that adds 10 times extracts 2 times, and each 1.5hr is put cold after-filtration except precipitation;
(2) filtrate is gone up the AB-8 macroporous adsorbent resin, 4 times of volumes are washed 1 time, use 90% ethanol elution of 4 times of volumes then, and concentrate eluant is to 500L;
(3) spray drying obtains dry powder 20kg; Add L-cysteine, Semen Maydis polypeptide, obtain pharmaceutical composition of the present invention, pharmaceutical composition being pulverized is fine powder again, sieve, the food stage cyclodextrin that adds additive of tablet 4kg again is pressed into every tablet of tablet that weighs 1.0g, namely gets the tablet of pharmaceutical composition of the present invention.Each edible 2.The prepared tablet of present embodiment should meet " the listed quality standard of 2005 editions one appendix ID of Chinese pharmacopoeia " tablet ".
The granule of embodiment 2 pharmaceutical compositions of the present invention
The component that comprises following weight portion:
Pericarpium Citri tangerinae 55kg, Folium Camelliae sinensis 25kg, Radix Puerariae 65kg, Semen Hoveniae (Fructus Hoveniae) 55kg, Rhizoma Imperatae 75kg, Herba Lophatheri 75kg, Radix Glycyrrhizae 55kg, Fructus Canarii albi fruit (life) 98kg, Fructus clausenae lansii (life) 98kg, L-cysteine 0.65kg, Semen Maydis polypeptide 4.0kg.
Preparation method is:
(1) will be except the said components mixing of L-cysteine and Semen Maydis polypeptide, the rustless steel that adds 2 tons extracts pot, and the water boil that adds 8 times extracts 2 times, and each 2hr is put cold after-filtration except precipitation;
(2) filtrate is gone up the AB-8 macroporous adsorbent resin, 5 times of volumes are washed 2 times, use 90% ethanol elution of 5 times of volumes then, and concentrate eluant is to 500L;
(3) spray drying obtains dry powder 20kg; Add L-cysteine, Semen Maydis polypeptide, namely get pharmaceutical composition of the present invention.It is fine powder that pharmaceutical composition is pulverized, and sieves, and adds proper auxiliary materials to make the granular formulations with certain particle size, then gets granule.The prepared granule of present embodiment should meet " the listed quality standard of 2005 editions one appendix IC of Chinese pharmacopoeia " granule ".
The capsule of embodiment 3 pharmaceutical compositions of the present invention
The component that comprises following weight portion:
Pericarpium Citri tangerinae 65kg, Folium Camelliae sinensis 35kg, Radix Puerariae 75kg, Semen Hoveniae (Fructus Hoveniae) 65kg, Rhizoma Imperatae 85kg, Herba Lophatheri 65kg, Radix Glycyrrhizae 45kg, Fructus Canarii albi fruit (life) 100kg, Fructus clausenae lansii (life) 100kg, L-cysteine 1.0kg, Semen Maydis polypeptide 5.0kg.
Preparation method is:
(1) will be except the said components mixing of L-cysteine and Semen Maydis polypeptide, the rustless steel that adds 2 tons extracts pot, and the water boil that adds 12 times extracts 3 times, and each 1.5hr is put cold after-filtration except precipitation;
(2) filtrate is gone up the AB-8 macroporous adsorbent resin, 6 times of volumes are washed 1 time, use 90% ethanol elution of 6 times of volumes then, and concentrate eluant is to 500L;
(3) spray drying obtains dry powder 20kg; Add L-cysteine, Semen Maydis polypeptide, namely get pharmaceutical composition of the present invention, add proper auxiliary materials again and be filled in Capsules or be sealed in the soft capsule material, then hard capsule, soft capsule (soft gelatin capsule) and enteric coated capsule.The prepared capsule of present embodiment should meet " the listed quality standard of 2005 editions one appendix IL of Chinese pharmacopoeia " capsule ".
The pharmaceutical composition of embodiment 1-3 is carried out the test of the drunk time of drunk mice, result's pharmaceutical composition of embodiment 1-3 as can be known all has the effect of relieving the effect of alcohol, wherein the effect of embodiment 1 (being optimum formula) is best, can significantly shorten the drunk time of drunk mice.
The human body of the pharmaceutical composition of embodiment 4 embodiment 1 effect observation that relieves the effect of alcohol
Have 20 people to take the tablet that relieves the effect of alcohol of embodiment 1 voluntarily, the experimental observation that relieves the effect of alcohol is changed to index with common drunk phenomenons such as dyschromasia, clearheaded degree and capacity for liquor variations, carries out the relieve the effect of alcohol effect of tablet of overall merit.
Discovery has 16 people to drink to take 3 in back 2 hours, spend 2 hours after, face skin is ruddyly disappeared by original, and brains transfers waking state to from the original state of sleeping of partly knocking.Have 4 people drinking preceding 2 hours, take 2 in the tablet of relieving the effect of alcohol voluntarily, drinking amount increases by 40% than original, in addition, overdrinks after drinking, and sensation is drunk than not taking medicine originally and returned to form soon, also occurs feeling sick, drunk phenomenons such as vomiting and sleep.
The test of relieving the effect of alcohol of the pharmaceutical composition of embodiment 5 embodiment 1
1, test model
Acute drunk model manufacturing: get some of mices, male and female are half-and-half tested preceding fasting one night (12h).Each group is irritated stomach earlier and is given Chinese liquor 0.18mL/10g body weight, do not wake up with mice drunk (being righting reflex loss) 10min, and acute drunk model success.
2, test material
Get 100 in the tablet of relieving the effect of alcohol of embodiment 1, after it is pulverized, be dissolved in the 100ml sterilized water, be used for the test of relieving the effect of alcohol of present embodiment.
3, test method
Get 80 of mices, divide four groups at random, 20 every group, male and female half and half are grouped as follows:
Blank group: regularly give 0.18mL/10g body weight normal saline every day and gavage;
Model group: regularly give 0.18mL/10g body weight Red Star two steamed bread of corn every day and gavage, every day 2 times (at 8 o'clock in the morning, at 6 o'clock in afternoon);
Treatment group: regularly give 0.5mL/20g body weight sobering preparation every day and gavage, behind the 30min, give 0.18mL/10g body weight Red Star two steamed bread of corn and gavage;
Sobering preparation group: regularly give 0.5mL/20g body weight sobering preparation every day and gavage, a continuous week.After one week, with not commensurability preparation the acute renal failure mice is carried out perfusion treatment (2 1g/kgd, 2 3g/kgd, 2 5g/kgd).
4, result of the test
(1) sobering preparation is to the influence of mouse sleep time
The pharmacological action of ethanol and pharmacokinetics ethanol thereof belong to depressor of nerve centre, and is the most responsive with cerebral cortex and network structure.The increase of concentration of alcohol in the cerebral tissue can be disturbed the function of neurotransmitter and receptor thereof, the transmission of the impulsion that affects the nerves, and it is impaired the fine movement ability to occur, phenomenons such as dysequilibrium.Therefore, with a certain amount of ethanol mice is irritated stomach, irritate stomach in different time sections with sobering preparation again, reflect best administration time from the drunk length of one's sleep that mice shows.
Result of the test shows: (the wine group after elder generation's " medicine ": the average out to 3h length of one's sleep that generally is shorter than elder generation's " wine " back medicine group mice earlier in the wine group of " medicine " back the length of one's sleep of mice; Elder generation's " wine " back medicine group: average 6h), and behind sobering preparation filling stomach 30min, irritate the mouse sleep time the shortest (2.5h) of stomach again with two steamed bread of corn, and irritate again with the mouse sleep time of sobering preparation the longest (4h) with 15min behind the two steamed bread of corn filling stomach; The length of one's sleep, more short expression antialcoholism action was more good, so adopt in the acute alcohol liver damage modeling test earlier to irritate stomach with two steamed bread of corn again behind the sobering preparation filling stomach 30min.Hence one can see that, and sobering preparation can be crossed antagonism ethanol to central nervous system's inhibitory action, alleviates ethanol to the infringement of body, and the metabolism of acceleration of alcohol promotes the decomposition of ethanol to drain, and reduces concentration of ethanol in the blood, alleviates drunk degree, shortens the drunk time.
(2) sobering preparation is to the influence of hepatic tissue, nephridial tissue, cerebral tissue mda content
Lipid peroxide is the product that free radical acts on polyunsaturated fatty acid; the generation positive correlation of content and free radical; because the protective effect of the enzyme of body Wheat Protein system and non-enzyme system constantly produces free radical and constantly is eliminated again, both are in poised state.If this balance is destroyed, free radical generates too much or removed slow in vivo, just can cause histoorgan in disorder and induce an illness, and accelerates aging.A series of radical reactions on the unsaturated fatty acid covalent bond can cause lipid peroxidation, MDA is the end-product of unsaturated fatty acid peroxidation, its content in serum and tissue, indirect reaction disorganization degree during radical reaction, but the effectiveness of preliminary judgement sobering preparation hepatoprotective and to the influence of mice body thus.
Result of the test shows: in acute alcohol liver damage test, with the blank group relatively, in the model group in mouse kidney, the brain MDA content all have and extremely significantly raise (P＜0.01) and significantly rising (P＜0.05); Compare with model group, in the treatment group in mouse kidney, the brain MDA content remarkable decline (P＜0.05) all arranged and extremely significantly descend (P＜0.01), and in the sobering preparation group in mouse liver, kidney, the brain MDA content be significantly to descend (P＜0.05), significantly descend (P＜0.05) and extremely significantly decline (P＜0.01) respectively; The equal not statistically significant of MDA content (P＞0.05) in mouse liver, kidney, the brain in sobering preparation group and the blank group.
This shows that sobering preparation is to being that acute alcohol liver damage or the disease of chronic alcoholism all have therapeutical effect to a certain extent.
(3) sobering preparation is to the influence of serum glutamic pyruvic transminase and glutamic oxaloacetic transaminase, GOT vigor
The main metabolic place of ethanol is liver, so the most serious internal organs of getting involved during acute alcoholism are livers.Glutamate pyruvate transaminase (GOT) and glutamic oxaloacetic transaminase, GOT (GPT) determination of activity are the indexs of the domestic reflection hepatocellular damage that is most widely used up to now.Can seek sobering preparation to the influence of the test of acute alcohol liver damage and chronic alcoholism test mice liver by glutamate pyruvate transaminase (GOT) and glutamic oxaloacetic transaminase, GOT (GPT) vigor in the mensuration serum, infer whether it also has the certain protection effect to liver in relieving alcoholic intoxication.
Glutamate pyruvate transaminase (GOT) and glutamic oxaloacetic transaminase, GOT (GPT) are distributed widely in various histoorgans in the humans and animals body, but how abundant with the content in the hepatocyte, are the main sources of these enzymes in the blood.ALT mainly is present in the liver cytoplasm, and AST only accounts for 20% in liver cytoplasm, and all the other 80% are present in the mitochondrion.The ratio of GPT/GOT is 0.6: 1 in liver cytoplasm, and ratio between two is 3: 1 in whole hepatocyte.When hepatocellular damage was serious, GPT was released into blood in the mitochondrion, and serum GPT is raise greater than GOT.Transaminase's content is about 100 times of content in the blood in the normal liver, so during the liver material injury, as long as 1% hepatic necrosis, increase by 1 times but sero-enzyme is active just the time.When permeability of cell membrane increased, even there is not necrosis, the transaminase in the hepatocyte can also be released into blood along concentration difference.Therefore the transaminase is the sensitivity sign of hepatocellular damage, also is the most responsive index of hepatic injury due to the ethanol, to understanding hepatocellular degree of injury and judging that the liver function situation has important value.
Shown by result of the test: in the test of acute alcohol liver damage, compare with the blank group, model group mice serum GOT, GPT level all have rising, have statistical significance (P＜0.01), and the existing damage of model group mouse liver cell is described.Compare with the blank group; treatment group mice serum GOT, GPT level have rising; has statistical significance (P＜0.05); and compare with model group; treatment group mice serum GOT, GPT level all have extremely significantly descend (P＜0.01); explanation is under the relieving alcoholic intoxication Chinese medicine preparation is intervened, and treatment group mouse liver cell degree of injury alleviates, and it has protective effect to acute alcohol liver damage mouse liver.Sobering preparation group and blank group mice serum GOT, the equal not statistically significant of GPT level (P＞0.05) illustrate that sobering preparation is to the harmless effect of mouse liver.
This shows that sobering preparation liver is had the certain protection effect, so the deducibility sobering preparation can reach liver protection function to a certain degree alleviating the degree of injury that ethanol causes hepatocyte down.
(4), sobering preparation is to the influence of liver, kidney, brain internal organs and tissue triglycerides content thereof
Hepatocyte fat become be alcoholic liver injury the earliest and one of the pathological change of the most normal appearance.Enter the ethanol of human body, in the liver metabolism process, can suppress the mitochondrion tricarboxylic acid cycle, make fatty acid metabolism generation obstacle, oxidation reduction, the property athero is in hepatocyte among continuing; Suppress lipase active in the hepatocyte, the lipolytic ability of hepatocyte descends; Heavy drinking stimulates adrenal gland and hypophysis hypothalamic pituitary adrenal axis, and the decomposition of fatty tissue around mobilizing discharges abundant fatty acid and enters liver, makes synthetic the increasing of the interior triacylglycerol (TG) of liver; Metabolite acetaldehyde infringement Golgi body function can not be transported away TG, and everything all can cause the formation of fatty liver.
Common hepatocyte enlargement during alcoholic liver injury, endochylema is loose, is hydropic degeneration; Simultaneously, the hepatocyte microtubule is impaired, and secretory functional disturbance causes secreted protein and stores, and hepatocyte is ballooning degeneration; Or the liver lipid metabolic disorder, athero in the hepatocyte, see in the endochylema be dispersed in or widely fat drip, hepatocyte is steatosis, all can cause the liver enlargement, volume increases, weight increases, the liver index increases.
Shown by result of the test: in acute alcohol liver damage test, with the blank group relatively, in the model group in mouse liver, the brain TG content all have extremely significantly and raise (P＜0.01), and in the relieving alcoholic intoxication Chinese medicine preparation group in the mouse liver TG content remarkable decline is arranged; With model group relatively, in the treatment group in the mouse brain TG content have extremely significantly and descend (P＜0.01), and in the relieving alcoholic intoxication Chinese medicine preparation group in the mouse brain TG content be extremely significantly decline (P＜0.01).TG content not statistically significant (P＞0.05) in mouse kidney, the brain in relieving alcoholic intoxication Chinese medicine preparation group and the blank group.
The acute toxicity test of the pharmaceutical composition of embodiment 6 embodiment 1
Get 100 in the tablet of relieving the effect of alcohol of embodiment 1, after it is pulverized, be dissolved in the 100ml sterilized water, be used for the acute toxicity test of present embodiment.
Select body weight 40 of the kunming mices of 18 ± 2g, divide 2 groups at random, 20 every group, male and female half and half, fasting one night (12h) before the test.Every group respectively with normal saline (blank group), sobering preparation 0.05g/mL, 0.5g/mL, 1g/mL concentration, presses 0.5mL/20g body weight gastric infusion, every day 2 times (at 8 o'clock in the morning, at 6 o'clock in afternoon), continuous 5 days.
Can be by gloss, the color and luster of its outward appearance physical behavior of observation such as chaeta, active state, the mental status etc. are learnt health status.After test, mice does not have death, and 0.05g/mL, 0.5g/mL, 1g/mL sobering preparation group and normal saline group on physical behavior (blank group) there is no significant difference, dissect the back perusal and do not see that its histoorgan is unusual; With normal saline group (blank group) relatively, 0.05g/mL, 0.5g/mL, 1g/mL sobering preparation group mice serum ALT, the equal not statistically significant of AST level, the deducibility sobering preparation is not had toxicity and to the effect of liver not damaged thus.
Although embodiment 4-6 adopts tablet to experimentize, other peroral dosage forms of pharmaceutical composition of the present invention as pill, granule, capsule, tablet etc., also can be obtained the effect of similarly relieving the effect of alcohol of embodiment 4-6.
The above embodiment has only expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to claim of the present invention.Should be pointed out that for the person of ordinary skill of the art without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (4)
1. one kind is used for the pharmaceutical composition relieve the effect of alcohol, it is characterized in that, by weight, comprises following component:
Pericarpium Citri tangerinae 55-65 part, Folium Camelliae sinensis 25-35 part, Radix Puerariae 65-75 part, Semen Hoveniae (Fructus Hoveniae) 55-65 part, Rhizoma Imperatae 75-85 part, Herba Lophatheri 65-75 part, Radix Glycyrrhizae 45-55 part, living Fructus Canarii albi fruit 95-105 part, SHENGHUANG follicarpium 95-105 part, L-cysteine 0.6-1.0 part, Semen Maydis polypeptide 4.0-5.0 part.
2. according to claim 1 be used to the pharmaceutical composition that relieves the effect of alcohol, it is characterized in that, comprise following component: 60 parts of Pericarpium Citri tangerinaes, 30 parts of Folium Camelliae sinensis, 70 parts of Radix Puerariaes, 60 parts of Semen Hoveniae (Fructus Hoveniae)s, 80 parts of Rhizoma Imperataes, 70 parts of Herba Lophatheris, 50 parts in Radix Glycyrrhizae, 100 parts of living Fructus Canarii albi fruits, 100 parts of SHENGHUANG follicarpiums, 0.8 part of L-cysteine, 4.5 parts of Semen Maydis polypeptides.
3. according to claim 1 and 2ly it is characterized in that be used to the pharmaceutical composition that relieves the effect of alcohol described pharmaceutical composition is tablet, granule, capsule, pill or powder.
4. claim 1 or 2 describedly is characterized in that be used to the preparation of drug combination method of relieving the effect of alcohol, and may further comprise the steps:
(1) with the component mixing except L-cysteine and Semen Maydis polypeptide, add 8-12 water boil doubly and extract 2-3 time, each 1.5-2hr is put cold after-filtration except precipitation;
(2) filtrate is gone up the AB-8 macroporous adsorbent resin, 4-6 times of column volume washing, then with 4-6 times of column volume 85-95% ethanol elution, concentrate eluant to a three/volume, spray drying obtains dry powder;
(3) in above-mentioned dry powder, add L-cysteine, Semen Maydis polypeptide, namely.
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| CN103636836B (en) * | 2013-11-25 | 2016-02-17 | 广西博士海意信息科技有限公司 | A kind of conditioning health protection tea |
| CN103598329A (en) * | 2013-11-26 | 2014-02-26 | 成冠辉 | Rapid preparation method of tomato hangover alleviating yoghurt |
| CN103598330A (en) * | 2013-11-26 | 2014-02-26 | 牙政鹏 | Rapid preparation method of olive hangover alleviating yoghurt |
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| CN108653716A (en) * | 2018-07-20 | 2018-10-16 | 武汉友酵生物技术有限公司 | A kind of tealeaves essence drunk-sobering tablet and preparation method thereof |
| CN109432387A (en) * | 2018-12-21 | 2019-03-08 | 陕西师范大学 | It is a kind of with the Chinese medicine-peptide combinations and preparation method thereof for resisting drunk effect |
| CN109758570A (en) * | 2019-03-27 | 2019-05-17 | 汤臣倍健股份有限公司 | A kind of pair of alcoholic liver injury has the composition and its health care product, application of auxiliary protection function |
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Application publication date: 20130717 |