KR20220153685A - 미세유체 디바이스들 및 키트들 및 그것의 사용을 위한 방법들 - Google Patents

미세유체 디바이스들 및 키트들 및 그것의 사용을 위한 방법들 Download PDF

Info

Publication number
KR20220153685A
KR20220153685A KR1020227038681A KR20227038681A KR20220153685A KR 20220153685 A KR20220153685 A KR 20220153685A KR 1020227038681 A KR1020227038681 A KR 1020227038681A KR 20227038681 A KR20227038681 A KR 20227038681A KR 20220153685 A KR20220153685 A KR 20220153685A
Authority
KR
South Korea
Prior art keywords
capture structure
situ generated
modified
microfluidic device
assay
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
KR1020227038681A
Other languages
English (en)
Korean (ko)
Inventor
크리스틴 지 보몬트
피터 제이 비밀러
볼커 엘 에스 커즈
그레고리 지 라비유
샤오화 왕
아타반 카루나카란
Original Assignee
버클리 라잇츠, 인크.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 버클리 라잇츠, 인크. filed Critical 버클리 라잇츠, 인크.
Publication of KR20220153685A publication Critical patent/KR20220153685A/ko
Withdrawn legal-status Critical Current

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545—Synthetic resin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424—Dielectrophoretic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0427—Electrowetting

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Fluid Mechanics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Optical Measuring Cells (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
KR1020227038681A 2015-12-08 2016-12-07 미세유체 디바이스들 및 키트들 및 그것의 사용을 위한 방법들 Withdrawn KR20220153685A (ko)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201562264665P 2015-12-08 2015-12-08
US62/264,665 2015-12-08
US201662333821P 2016-05-09 2016-05-09
US62/333,821 2016-05-09
US201662418625P 2016-11-07 2016-11-07
US62/418,625 2016-11-07
KR1020187019334A KR102466814B1 (ko) 2015-12-08 2016-12-07 미세유체 디바이스들 및 키트들 및 그것의 사용을 위한 방법들
PCT/US2016/065426 WO2017100347A1 (en) 2015-12-08 2016-12-07 Microfluidic devices and kits and methods for use thereof

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020187019334A Division KR102466814B1 (ko) 2015-12-08 2016-12-07 미세유체 디바이스들 및 키트들 및 그것의 사용을 위한 방법들

Publications (1)

Publication Number Publication Date
KR20220153685A true KR20220153685A (ko) 2022-11-18

Family

ID=57590894

Family Applications (2)

Application Number Title Priority Date Filing Date
KR1020187019334A Active KR102466814B1 (ko) 2015-12-08 2016-12-07 미세유체 디바이스들 및 키트들 및 그것의 사용을 위한 방법들
KR1020227038681A Withdrawn KR20220153685A (ko) 2015-12-08 2016-12-07 미세유체 디바이스들 및 키트들 및 그것의 사용을 위한 방법들

Family Applications Before (1)

Application Number Title Priority Date Filing Date
KR1020187019334A Active KR102466814B1 (ko) 2015-12-08 2016-12-07 미세유체 디바이스들 및 키트들 및 그것의 사용을 위한 방법들

Country Status (12)

Country Link
US (3) US10705082B2 (enExample)
EP (2) EP3387438B1 (enExample)
JP (2) JP7083752B2 (enExample)
KR (2) KR102466814B1 (enExample)
CN (2) CN108603878A (enExample)
AU (1) AU2016365724B2 (enExample)
CA (1) CA3005077C (enExample)
DK (1) DK3387438T3 (enExample)
IL (2) IL259837B2 (enExample)
SG (1) SG11201804275TA (enExample)
TW (2) TWI847100B (enExample)
WO (1) WO2017100347A1 (enExample)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2016361413B2 (en) 2015-11-23 2022-04-14 Berkeley Lights, Inc. In situ-generated microfluidic isolation structures, kits and methods of use thereof
AU2017248822B2 (en) 2016-04-15 2022-09-15 Berkeley Lights, Inc. Methods, systems and kits for in-pen assays
JP7356351B2 (ja) 2016-12-12 2023-10-04 エクセラ・バイオサイエンシーズ・インコーポレイテッド マイクロキャピラリーアレイを使用したスクリーニングのための方法およびシステム
WO2018125832A1 (en) 2016-12-30 2018-07-05 xCella Biosciences, Inc. Multi-stage sample recovery system
WO2018165432A1 (en) * 2017-03-08 2018-09-13 The Regents Of The University Of Michigan Functionalization of polymer scaffolds
JP7227162B2 (ja) 2017-06-06 2023-02-21 ザイマージェン インコーポレイテッド 真菌株を改良するためのｈｔｐゲノム操作プラットフォーム
NZ759924A (en) * 2017-08-01 2023-07-28 Illumina Inc Hydrogel beads for nucleotide sequencing
DE102017121914A1 (de) * 2017-09-21 2019-03-21 Endress+Hauser Conducta Gmbh+Co. Kg Sensorelement und Verfahren zum Herstellen eines Sensorelements
WO2019075476A2 (en) 2017-10-15 2019-04-18 Berkeley Lights, Inc. METHODS, SYSTEMS AND KITS FOR ENCLOSED TESTS
TWI652658B (zh) 2017-12-12 2019-03-01 奇景光電股份有限公司 晶片以及使用其的電子裝置
WO2019133874A1 (en) * 2017-12-31 2019-07-04 Berkeley Lights, Inc. General functional assay
EP4083225A1 (en) 2018-02-13 2022-11-02 Illumina, Inc. Dna sequencing using hydrogel beads
WO2019204229A1 (en) 2018-04-20 2019-10-24 Illumina, Inc. Methods of encapsulating single cells, the encapsulated cells and uses thereof
JP2021526799A (ja) 2018-06-06 2021-10-11 ザイマージェン インコーポレイテッド 発酵および産生中の真菌の形態を制御するためのシグナル伝達に関与する遺伝子の操作
US20210162413A1 (en) * 2018-06-18 2021-06-03 Hewlett-Packard Development Company, L.P. Microfluidic immunoassays
AU2019358198A1 (en) * 2018-10-11 2021-05-27 Berkeley Lights, Inc. Systems and methods for identification of optimized protein production and kits therefor
CN111036315B (zh) * 2018-10-15 2021-09-21 京东方科技集团股份有限公司 一种微量样品的生成方法及生成芯片
EP3870721B1 (en) 2018-10-26 2025-08-13 Illumina, Inc. Modulating polymer beads for dna processing
KR20210089193A (ko) 2018-11-01 2021-07-15 버클리 라잇츠, 인크. 미세유체 디바이스에서 생물학적 세포를 분석하기 위한 방법
ES3039910T3 (en) 2018-12-06 2025-10-27 Xcella Biosciences Inc Lateral loading of microcapillary arrays
JP7231923B2 (ja) * 2019-01-15 2023-03-02 国立大学法人東京工業大学 複合体の検出方法、並びにそれに用いる担体及び検出キット
WO2020160472A1 (en) * 2019-02-01 2020-08-06 Sentilus Holdco, Llc High-sensitivity assay
CN110054146B (zh) * 2019-03-18 2021-08-24 北京航空航天大学 一种周期性v型微柱结构复合膜及其制备方法与应用
CN114126762B (zh) * 2019-04-30 2023-01-03 伯克利之光生命科技公司 用于包封和测定细胞的方法
US11668676B2 (en) * 2019-05-30 2023-06-06 The Regents Of The University Of California Microfluidic acoustic devices and methods
WO2021067401A1 (en) * 2019-09-30 2021-04-08 University Of Florida Research Foundation, Incorporated Use of ganglioside 2 and 3 for circulating sarcoma cell detection
TW202142856A (zh) 2019-11-17 2021-11-16 美商伯克利之光生命科技公司 用於生物樣本之分析的系統及方法
CN111046607B (zh) * 2019-12-12 2025-05-13 浙江清华柔性电子技术研究院 柔性微流体器件设计方法及柔性微流体器件
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
US20230405581A1 (en) * 2020-11-17 2023-12-21 Xbiologix, Inc. Configurable substrate of a fluidic device
US12377415B2 (en) 2020-12-30 2025-08-05 The Trustees Of The University Of Pennsylvania Photoactivated selective release (PHASR) of droplets from microwell arrays
TW202248635A (zh) * 2021-04-06 2022-12-16 美商伯克利之光生命科技公司 微流體分析與來自非哺乳動物細胞之生物生產的方法及用於其之套組
US12465910B2 (en) * 2021-12-10 2025-11-11 Bio-Rad Laboratories, Inc. Compositions, methods, and systems for sample processing with morphology-adjustable functionalized particles
EP4469207A1 (en) * 2022-01-25 2024-12-04 Bruker Cellular Analysis, Inc. Methods of assaying micro-objects in a microfluidic device
WO2024108068A2 (en) * 2022-11-17 2024-05-23 Bruker Cellular Analysis, Inc. Methods of evaluating virus-producing cells
WO2025068547A1 (en) * 2023-09-28 2025-04-03 Zhen Zhang A microfluidic device, a microfluidic system and a method for the capture and controllable release of single biological cells

Family Cites Families (113)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2288760T3 (es) 1996-04-25 2008-01-16 Bioarray Solutions Ltd. Ensamblaje electrocinetico controlado por luz de particulas proximas a superficies.
US6387707B1 (en) 1996-04-25 2002-05-14 Bioarray Solutions Array Cytometry
JP2002536640A (ja) 1999-02-03 2002-10-29 アクララ バイオサイエンシーズ， インコーポレイテッド 微小流体導入の際のマルチチャネル制御
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
US6942776B2 (en) 1999-05-18 2005-09-13 Silicon Biosystems S.R.L. Method and apparatus for the manipulation of particles by means of dielectrophoresis
EP1065378B1 (en) 1999-06-28 2002-04-03 California Institute of Technology Microfabricated elastomeric valve and pump systems
KR100677860B1 (ko) 1999-07-23 2007-02-05 보드 오브 트러스티스 오브 유니버스티 오브 일리노이즈 미세하게 제조되는 장치 및 그의 제조방법
US6794196B2 (en) 1999-12-20 2004-09-21 The Penn State Research Foundation Deposited thin films and their use in detection, attachment and bio-medical applications
US20030007894A1 (en) 2001-04-27 2003-01-09 Genoptix Methods and apparatus for use of optical forces for identification, characterization and/or sorting of particles
AU2002230524A1 (en) * 2000-11-16 2002-05-27 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
ITTO20010411A1 (it) 2001-05-02 2002-11-02 Silicon Biosystems S R L Metodo e dispositivo per l'esecuzione di test e saggi ad alta processivita' ed alto valore biologico su cellule e/o composti.
US20030175947A1 (en) 2001-11-05 2003-09-18 Liu Robin Hui Enhanced mixing in microfluidic devices
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
WO2003095088A1 (en) * 2002-05-07 2003-11-20 Japan Science And Technology Agency Method and apparatus for collecting micromaterial
US6958132B2 (en) 2002-05-31 2005-10-25 The Regents Of The University Of California Systems and methods for optical actuation of microfluidics based on opto-electrowetting
DE10224352A1 (de) * 2002-06-01 2003-12-11 Mueller Schulte Detlef Thermosensitive Polymerträger mit veränderbarer physikalischer Struktur für die biochemische Analytik, Diagnostik und Therapie
US7790443B2 (en) 2002-08-27 2010-09-07 Vanderbilt University Bioreactors with substance injection capacity
KR101216828B1 (ko) 2002-12-30 2013-01-04 더 리전트 오브 더 유니버시티 오브 캘리포니아 병원균 검출과 분석을 위한 방법과 기구
CN101613660B (zh) * 2002-12-30 2013-05-15 加州大学评议会 检测和分析病原体的方法和设备
WO2004065618A2 (en) 2003-01-16 2004-08-05 Thermogenic Imaging Methods and devices for monitoring cellular metabolism in microfluidic cell-retaining chambers
AU2004228678A1 (en) 2003-04-03 2004-10-21 Fluidigm Corp. Microfluidic devices and methods of using same
US20060240548A1 (en) 2003-06-26 2006-10-26 Mordechai Deutsch Materials for constructing cell-chips, cell-chip covers, cell-chips coats, processed cell-chips and uses thereof
US20050164402A1 (en) 2003-07-14 2005-07-28 Belisle Christopher M. Sample presentation device
EP1664265A4 (en) 2003-07-21 2009-11-25 Seng Entpr Ltd IMPROVED MULTIPLAY PLATE
US20060194306A1 (en) * 2003-08-25 2006-08-31 Herr Amy E System for gel electrophoretic immunoassay
JP4328168B2 (ja) 2003-10-02 2009-09-09 ソニー株式会社 毛細管現象を利用する物質間の相互作用検出部と該検出部を用いる方法及びバイオアッセイ用基板
AU2004284367B2 (en) * 2003-10-29 2009-03-12 Agency For Science, Technology And Research Method for detecting analytes by means of an analyte/polymeric activator bilayer arrangement
WO2005072399A2 (en) 2004-01-29 2005-08-11 Massachusetts Institute Of Technology Microscale sorting cytometer
US8765454B2 (en) 2004-02-18 2014-07-01 Xiaochuan Zhou Fluidic devices and methods for multiplex chemical and biochemical reactions
US20050221473A1 (en) 2004-03-30 2005-10-06 Intel Corporation Sensor array integrated circuits
WO2005100541A2 (en) 2004-04-12 2005-10-27 The Regents Of The University Of California Optoelectronic tweezers for microparticle and cell manipulation
US7935479B2 (en) 2004-07-19 2011-05-03 Cell Biosciences, Inc. Methods and devices for analyte detection
US7258990B2 (en) * 2004-08-19 2007-08-21 Biocept, Inc. Alleviation of non-specific binding in microarray assays
WO2006047591A2 (en) 2004-10-25 2006-05-04 University Of Washington Rapid microfluidic assay for analyte interactions
US20060091015A1 (en) 2004-11-01 2006-05-04 Applera Corporation Surface modification for non-specific adsorption of biological material
US20060237080A1 (en) * 2005-02-15 2006-10-26 Sangyong Jon Patterned surfaces and polymeric microstructures within robust microfluidic channels
JP2006308332A (ja) 2005-04-26 2006-11-09 Moritex Corp 固定反応物質と液体反応物質との反応用フローセル及び反応装置並びに反応装置の運転方法
TWI265864B (en) 2005-05-20 2006-11-11 Univ Nat Cheng Kung Hydrophilic surface structure of non-hydrophilic substrate and fabricating method for the same
EP3029135B1 (en) 2005-07-07 2021-03-17 The Regents of the University of California Apparatus for cell culture array
CA2616504A1 (en) 2005-08-19 2007-03-01 The Regents Of The University Of California Microfluidic methods for diagnostics and cellular analysis
US20070095667A1 (en) 2005-10-27 2007-05-03 Applera Corporation Optoelectronic Separation of Biomolecules
TW200734641A (en) * 2005-12-26 2007-09-16 Inst Of Microchemical Technology Microchip for immunoassay, kit for immunoassay and immunoassay method
US20090011486A1 (en) * 2006-01-12 2009-01-08 Massachusetts Institute Of Technology Biodegradable Elastomers
WO2007092713A2 (en) 2006-02-02 2007-08-16 Trustees Of The University Of Pennsylvania Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease
US8124015B2 (en) 2006-02-03 2012-02-28 Institute For Systems Biology Multiplexed, microfluidic molecular assay device and assay method
AU2007212306A1 (en) 2006-02-07 2007-08-16 Wafergen, Inc. Temperature-regulated culture plates
US8980198B2 (en) 2006-04-18 2015-03-17 Advanced Liquid Logic, Inc. Filler fluids for droplet operations
US20150107995A1 (en) 2006-04-18 2015-04-23 Advanced Liquid Logic, Inc. Droplet Actuator Devices and Methods for Manipulating Beads
WO2010048279A2 (en) * 2008-10-21 2010-04-29 Massachusetts Institute Of Technology Lock-release polymerization
US7790631B2 (en) 2006-11-21 2010-09-07 Intel Corporation Selective deposition of a dielectric on a self-assembled monolayer-adsorbed metal
US20080153134A1 (en) 2006-12-22 2008-06-26 Willy Wiyatno Methods and apparatus for generating hydrophilic patterning of high density microplates using an amphiphilic polymer
WO2008083323A1 (en) 2006-12-29 2008-07-10 Invitrogen Corporation Detection apparatus
EP2125219B1 (en) 2007-01-19 2016-08-10 Fluidigm Corporation High precision microfluidic devices and methods
US8685344B2 (en) 2007-01-22 2014-04-01 Advanced Liquid Logic, Inc. Surface assisted fluid loading and droplet dispensing
WO2008119066A1 (en) 2007-03-28 2008-10-02 The Regents Of The University Of California Single-sided lateral-field and phototransistor-based optoelectronic tweezers
WO2008131048A2 (en) * 2007-04-16 2008-10-30 Cellpoint Diagnotics, Inc. Devices and methods for diagnosing, prognosing, or theranosing a condition by enriching rare cells
ATE554859T1 (de) 2007-05-24 2012-05-15 Univ California Integrierte fluidische vorrichtungen mit magnetischer sortierung
WO2009021232A2 (en) 2007-08-09 2009-02-12 Massachusetts Institute Of Technology High-throughput, whole-animal screening system
WO2009061392A1 (en) 2007-11-05 2009-05-14 President And Fellows Of Harvard College Forming gel structures using microfluidic channels
EP2212437A4 (en) 2007-11-07 2011-09-28 Univ British Columbia MICROFLUIDIC DEVICE AND METHOD OF USING THE DEVICE
US20110117634A1 (en) 2008-04-21 2011-05-19 Asaf Halamish Flat cell carriers with cell traps
CN101275114A (zh) 2008-04-22 2008-10-01 北京大学 一种微流细胞培养阵列及其应用
KR100991752B1 (ko) 2008-07-15 2010-11-03 한국과학기술원 단일 평면 광전자 소자를 이용한 미세입자 구동장치 및구동방법
GB2464300A (en) 2008-10-10 2010-04-14 Univ Dublin City Microfluidic multiplexed cellular and molecular analysis device and method
CN102449163A (zh) 2009-04-03 2012-05-09 加利福尼亚大学董事会 分选细胞和其它生物微粒的方法和装置
US20100273681A1 (en) 2009-04-27 2010-10-28 Wisconsin Alumni Research Foundation Combinatorial chemistry reaction cell with optical tweezers
GB0909923D0 (en) 2009-06-09 2009-07-22 Oxford Gene Tech Ip Ltd Picowell capture devices for analysing single cells or other particles
JP2011000079A (ja) 2009-06-19 2011-01-06 Univ Of Tokyo 粒子を操作する方法及びマイクロ流体装置
US20110186165A1 (en) 2009-10-05 2011-08-04 Borenstein Jeffrey T Three-dimensional microfluidic platforms and methods of use and manufacture thereof
US20130118905A1 (en) 2010-05-26 2013-05-16 Tosoh Corporation Biological sample immobilizing apparatus
CH703278B1 (fr) 2010-06-15 2016-11-15 Csem Centre Suisse D'electronique Et De Microtechnique Sa - Rech Et Développement Appareil et plateforme pour analyse multiplex.
CN101947124B (zh) 2010-06-25 2012-07-04 博奥生物有限公司 一种集成式微流控芯片装置及其使用方法
US20130115606A1 (en) 2010-07-07 2013-05-09 The University Of British Columbia System and method for microfluidic cell culture
US10087408B2 (en) 2010-07-07 2018-10-02 The University Of British Columbia System and method for microfluidic cell culture
US9188593B2 (en) 2010-07-16 2015-11-17 The University Of British Columbia Methods for assaying cellular binding interactions
US9533306B2 (en) 2010-08-02 2017-01-03 The Regents Of The University Of California Single sided continuous optoelectrowetting (SCEOW) device for droplet manipulation with light patterns
US20120040843A1 (en) * 2010-08-12 2012-02-16 Dublin City University Centrifugal capture system
US9133499B2 (en) 2010-09-14 2015-09-15 The Regents Of The University Of California Method and device for isolating cells from heterogeneous solution using microfluidic trapping vortices
EP2622342A4 (en) 2010-09-29 2016-12-14 Massachusetts Inst Technology DEVICE FOR THE INVESTIGATION OF CELLULAR INTERACTIONS WITH HIGH THROUGHPUT
US9476811B2 (en) 2010-10-01 2016-10-25 The Governing Council Of The University Of Toronto Digital microfluidic devices and methods incorporating a solid phase
GB2499340B (en) 2010-10-04 2015-10-28 Genapsys Inc Methods for sequencing nucleic acids
US8581167B2 (en) 2010-11-16 2013-11-12 Palo Alto Research Center Incorporated Optically patterned virtual electrodes and interconnects on polymer and semiconductive substrates
CN103392124B (zh) 2010-12-03 2016-04-20 塞普莱有限公司 细胞功能的微分析
US10571475B2 (en) 2010-12-03 2020-02-25 Cellply S.R.L. Rapid screening of monoclonal antibodies
WO2012109068A2 (en) 2011-02-11 2012-08-16 Corning Incorporated Enzyme cleavable cell release polymeric surface
JP5093633B2 (ja) * 2011-02-28 2012-12-12 独立行政法人科学技術振興機構 複数物質応答性ゲルおよびその製造方法並びにその利用
CN116179323A (zh) 2011-05-27 2023-05-30 不列颠哥伦比亚大学 用于高通量分析的微流控细胞捕获和分析设备
US9227200B2 (en) 2011-06-03 2016-01-05 The Regents Of The University Of California Microfluidic devices with flexible optically transparent electrodes
WO2013003624A2 (en) 2011-06-29 2013-01-03 Academia Sinica The capture, purification and release of biological substance using a surface coating
EP2739587B1 (en) 2011-08-01 2020-05-27 Denovo Sciences Cell capture system
US9050593B2 (en) 2011-11-23 2015-06-09 Wisconsin Alumni Research Foundation Self-loading microfluidic device and methods of use
US9304065B2 (en) 2012-02-29 2016-04-05 Fluidigm Corporation Methods, systems and devices for multiple single-cell capturing and processing using microfluidics
US10626435B2 (en) 2012-03-28 2020-04-21 Northeastern University Nanofluidic device for isolating, growing, and characterizing microbial cells
KR101959447B1 (ko) 2012-04-06 2019-03-18 삼성전자주식회사 시료 중의 표적물질을 효율적으로 처리하는 방법
TWI512383B (zh) 2012-07-04 2015-12-11 Ind Tech Res Inst 光學感應式介電泳裝置
US9857333B2 (en) 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects
US9403172B2 (en) 2012-11-08 2016-08-02 Berkeley Lights, Inc. Circuit based optoelectronic tweezers
US9366647B2 (en) 2013-03-14 2016-06-14 Taiwan Semiconductor Manufacturing Company, Ltd. Optical detection for bio-entities
US9239328B2 (en) 2012-12-17 2016-01-19 Taiwan Semiconductor Manufacturing Company, Ltd. Systems and methods for an integrated bio-entity manipulation and processing semiconductor device
CN109342710A (zh) 2013-03-28 2019-02-15 英属哥伦比亚大学 微流控装置及其在多细胞分泌检测中的使用方法
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
CA2927701C (en) 2013-10-22 2020-10-13 Berkeley Lights, Inc. Microfluidic devices having sequestration pens and methods of testing biological micro-objects with same
EP3783094B1 (en) 2013-10-22 2023-10-11 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
SG10202105400YA (en) * 2013-10-22 2021-07-29 Berkeley Lights Inc Exporting a selected group of micro-objects from a micro-fluidic device
US20150306598A1 (en) 2014-04-25 2015-10-29 Berkeley Lights, Inc. DEP Force Control And Electrowetting Control In Different Sections Of The Same Microfluidic Apparatus
CA2945177C (en) 2014-04-25 2021-12-21 Berkeley Lights, Inc. Providing dep manipulation devices and controllable electrowetting devices in the same microfluidic apparatus
US20150306599A1 (en) 2014-04-25 2015-10-29 Berkeley Lights, Inc. Providing DEP Manipulation Devices And Controllable Electrowetting Devices In The Same Microfluidic Apparatus
JP6514776B2 (ja) 2014-12-08 2019-05-15 バークレー ライツ，インコーポレイテッド 横方向／垂直方向トランジスタ構造を含むマイクロ流体デバイスならびにそれを作製および使用するプロセス
US9744533B2 (en) 2014-12-10 2017-08-29 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
AU2016250689B2 (en) 2015-04-22 2021-07-08 Berkeley Lights, Inc. Microfluidic cell culture
KR20230042540A (ko) 2015-12-30 2023-03-28 버클리 라잇츠, 인크. 광학적으로 구동되는 대류 및 변위를 위한 미세유체 디바이스들, 키트들 및 그 방법들
AU2017248822B2 (en) 2016-04-15 2022-09-15 Berkeley Lights, Inc. Methods, systems and kits for in-pen assays
AU2017271673B2 (en) 2016-05-26 2022-04-14 Berkeley Lights, Inc. Covalently modified surfaces, kits, and methods of preparation and use

Also Published As

Publication number Publication date
US20210011015A1 (en) 2021-01-14
AU2016365724A1 (en) 2018-06-21
US11454629B2 (en) 2022-09-27
US20230023831A1 (en) 2023-01-26
KR102466814B1 (ko) 2022-11-11
CA3005077C (en) 2023-02-28
CN114878807A (zh) 2022-08-09
EP3387438A1 (en) 2018-10-17
EP3387438B1 (en) 2023-03-01
US10705082B2 (en) 2020-07-07
CA3005077A1 (en) 2017-06-15
IL297205A (en) 2022-12-01
TW201727234A (zh) 2017-08-01
IL259837B2 (en) 2023-03-01
KR20180091062A (ko) 2018-08-14
JP7083752B2 (ja) 2022-06-13
IL259837B (en) 2022-11-01
TW202221322A (zh) 2022-06-01
TWI758265B (zh) 2022-03-21
TWI847100B (zh) 2024-07-01
DK3387438T3 (da) 2023-05-15
JP2018538531A (ja) 2018-12-27
JP2022112033A (ja) 2022-08-01
EP4102226A1 (en) 2022-12-14
CN108603878A (zh) 2018-09-28
AU2016365724B2 (en) 2023-10-12
US20170184583A1 (en) 2017-06-29
WO2017100347A1 (en) 2017-06-15
IL259837A (en) 2018-07-31
SG11201804275TA (en) 2018-06-28

Similar Documents

Publication Publication Date Title
KR102466814B1 (ko) 미세유체 디바이스들 및 키트들 및 그것의 사용을 위한 방법들
US20230182136A1 (en) In situ-generated microfluidic isolation structures, kits and methods of use thereof
KR102512607B1 (ko) 광학적으로 구동되는 대류 및 변위를 위한 미세유체 디바이스들, 키트들 및 그 방법들
KR102486307B1 (ko) 미세유체 디바이스에서의 t 림프구들의 선택 및 클로닝
HK40077731A (en) Microfluidic devices and kits and methods for use thereof
HK1261471A1 (en) Microfluidic devices and kits and methods for use thereof

Legal Events

Date Code Title Description
A107 Divisional application of patent
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20221103

Application number text: 1020187019334

Filing date: 20180705

PA0201 Request for examination
PG1501 Laying open of application
E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20230927

Patent event code: PE09021S01D

PC1202 Submission of document of withdrawal before decision of registration

Comment text: [Withdrawal of Procedure relating to Patent, etc.] Withdrawal (Abandonment)

Patent event code: PC12021R01D

Patent event date: 20231222

WITB Written withdrawal of application