KR20220023985A - 효율적인 피피알 단백질의 작제방법 및 그의 이용 - Google Patents

효율적인 피피알 단백질의 작제방법 및 그의 이용 Download PDF

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Publication number
KR20220023985A
KR20220023985A KR1020217040377A KR20217040377A KR20220023985A KR 20220023985 A KR20220023985 A KR 20220023985A KR 1020217040377 A KR1020217040377 A KR 1020217040377A KR 20217040377 A KR20217040377 A KR 20217040377A KR 20220023985 A KR20220023985 A KR 20220023985A
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Korean (ko)
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유스케 야지
타카히로 나카무라
Original Assignee
에딧포스 인코포레이티드
고쿠리쓰다이가쿠호진 규슈다이가쿠
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Publication of KR20220023985A publication Critical patent/KR20220023985A/ko
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/85—Fusion polypeptide containing an RNA binding domain

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
KR1020217040377A 2019-05-29 2020-05-29 효율적인 피피알 단백질의 작제방법 및 그의 이용 Pending KR20220023985A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JPJP-P-2019-100551 2019-05-29
JP2019100551 2019-05-29
PCT/JP2020/021472 WO2020241876A1 (ja) 2019-05-29 2020-05-29 効率的なｐｐｒタンパク質の作製方法及びその利用

Publications (1)

Publication Number Publication Date
KR20220023985A true KR20220023985A (ko) 2022-03-03

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KR1020217040377A Pending KR20220023985A (ko) 2019-05-29 2020-05-29 효율적인 피피알 단백질의 작제방법 및 그의 이용

Country Status (11)

Country Link
US (1) US20220289795A1 (https=)
EP (1) EP3978513A4 (https=)
JP (3) JP7491515B2 (https=)
KR (1) KR20220023985A (https=)
CN (1) CN114729017A (https=)
AU (2) AU2020283177B2 (https=)
BR (1) BR112021023878A2 (https=)
CA (1) CA3142299A1 (https=)
IL (1) IL288426A (https=)
SG (1) SG11202113055UA (https=)
WO (1) WO2020241876A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3977849A4 (en) * 2019-05-29 2023-06-28 Editforce, Inc. Ppr protein with less aggregation and use thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013058404A1 (ja) 2011-10-21 2013-04-25 国立大学法人九州大学 Pprモチーフを利用したrna結合性蛋白質の設計方法及びその利用
WO2014175284A1 (ja) 2013-04-22 2014-10-30 国立大学法人九州大学 Ｐｐｒモチーフを利用したｄｎａ結合性タンパク質およびその利用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002322469B2 (en) * 2001-07-12 2008-05-22 Dna Landmarks, Inc. Nuclear fertility restorer genes and methods of use in plants
JP2013128413A (ja) * 2010-03-11 2013-07-04 Kyushu Univ Ｐｐｒモチーフを利用したｒｎａ結合性蛋白質の改変方法
WO2017209122A1 (ja) 2016-06-03 2017-12-07 国立大学法人九州大学 標的ｍＲＮＡからのタンパク質発現量を向上させるための融合タンパク質
JPWO2018030488A1 (ja) * 2016-08-10 2019-06-13 富士フイルム和光純薬株式会社 Ｐｐｒモチーフを利用したｄｎａ結合性タンパク質およびその利用
US12441770B2 (en) * 2018-06-06 2025-10-14 The University Of Western Australia Proteins and their use for nucleotide binding

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013058404A1 (ja) 2011-10-21 2013-04-25 国立大学法人九州大学 Pprモチーフを利用したrna結合性蛋白質の設計方法及びその利用
WO2014175284A1 (ja) 2013-04-22 2014-10-30 国立大学法人九州大学 Ｐｐｒモチーフを利用したｄｎａ結合性タンパク質およびその利用

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
1. Coquille, S. et al. An Artificial PPR Scaffold for Programmable RNA Recognition. Nature Communications 5, Article number: 5729 (2014)
2. Shen, C. et al. Specific RNA Recognition by Designer Pentatricopeptide Repeat Protein. Molecular Plant 8, 667-670 (2015)
3. Shen, C. et al. Structural Basis for Specific single-stranded RNA Recognition by Designer Pentatricopeptide Repeat Proteins. Nature Communications, Volume 7, Article number: 11285 (2016)
4. Gully, B. S. et al. The Design and Structural Characterization of a Synthetic Pentatricopeptide Repeat Protein. Acta Cryst. D71, 196-208 (2015)
5. Miranda, R. G. et al. RNA-binding Specificity Landscapes of Designer Pentatricopeptide Repeat Proteins Elucidate Principles of PPR-RNA Interactions. Nucleic Acids Research, 46 (5), 2613-2623 (2018)
6. Yan, J. et al. Delineation of Pentatricopeptide Repeat Codes for Target RNA Prediction. Nucleic Acids Research, gkz 075 (2019)

Also Published As

Publication number Publication date
JP2025107351A (ja) 2025-07-17
AU2020283177A1 (en) 2022-01-06
JP7683865B2 (ja) 2025-05-27
JP7491515B2 (ja) 2024-05-28
JPWO2020241876A1 (https=) 2020-12-03
WO2020241876A1 (ja) 2020-12-03
NZ783345A (en) 2025-06-27
AU2025204992A1 (en) 2025-07-24
SG11202113055UA (en) 2021-12-30
EP3978513A1 (en) 2022-04-06
US20220289795A1 (en) 2022-09-15
EP3978513A4 (en) 2023-06-28
CN114729017A (zh) 2022-07-08
IL288426A (en) 2022-01-01
AU2020283177B2 (en) 2025-05-08
BR112021023878A2 (pt) 2022-01-25
CA3142299A1 (en) 2020-12-03
JP2023145672A (ja) 2023-10-11

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