AU2020283177B2 - Efficient method for preparing ppr protein and use of the same - Google Patents

Efficient method for preparing ppr protein and use of the same Download PDF

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Publication number
AU2020283177B2
AU2020283177B2 AU2020283177A AU2020283177A AU2020283177B2 AU 2020283177 B2 AU2020283177 B2 AU 2020283177B2 AU 2020283177 A AU2020283177 A AU 2020283177A AU 2020283177 A AU2020283177 A AU 2020283177A AU 2020283177 B2 AU2020283177 B2 AU 2020283177B2
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Australia
Prior art keywords
substitution
amino acid
seq
ppr
sequence
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AU2020283177A
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English (en)
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AU2020283177A1 (en
Inventor
Takahiro Nakamura
Yusuke Yagi
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Editforce Inc
Kyushu University NUC
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Editforce Inc
Kyushu University NUC
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Priority to AU2025204992A priority Critical patent/AU2025204992A1/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/85—Fusion polypeptide containing an RNA binding domain

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)
AU2020283177A 2019-05-29 2020-05-29 Efficient method for preparing ppr protein and use of the same Active AU2020283177B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2025204992A AU2025204992A1 (en) 2019-05-29 2025-06-30 Efficient method for preparing ppr protein and use of the same

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019-100551 2019-05-29
JP2019100551 2019-05-29
PCT/JP2020/021472 WO2020241876A1 (ja) 2019-05-29 2020-05-29 効率的なｐｐｒタンパク質の作製方法及びその利用

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2025204992A Division AU2025204992A1 (en) 2019-05-29 2025-06-30 Efficient method for preparing ppr protein and use of the same

Publications (2)

Publication Number Publication Date
AU2020283177A1 AU2020283177A1 (en) 2022-01-06
AU2020283177B2 true AU2020283177B2 (en) 2025-05-08

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AU2020283177A Active AU2020283177B2 (en) 2019-05-29 2020-05-29 Efficient method for preparing ppr protein and use of the same
AU2025204992A Pending AU2025204992A1 (en) 2019-05-29 2025-06-30 Efficient method for preparing ppr protein and use of the same

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Country Status (11)

Country Link
US (1) US20220289795A1 (https=)
EP (1) EP3978513A4 (https=)
JP (3) JP7491515B2 (https=)
KR (1) KR20220023985A (https=)
CN (1) CN114729017A (https=)
AU (2) AU2020283177B2 (https=)
BR (1) BR112021023878A2 (https=)
CA (1) CA3142299A1 (https=)
IL (1) IL288426A (https=)
SG (1) SG11202113055UA (https=)
WO (1) WO2020241876A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202113057XA (en) * 2019-05-29 2021-12-30 Editforce Inc Ppr protein causing less aggregation and use of the same

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030126646A1 (en) * 2001-07-12 2003-07-03 Brown Gregory G. Nuclear fertility restorer genes and methods of use in plants
WO2011111829A1 (ja) * 2010-03-11 2011-09-15 国立大学法人九州大学 Pprモチーフを利用したrna結合性蛋白質の改変方法
WO2013058404A1 (ja) * 2011-10-21 2013-04-25 国立大学法人九州大学 Pprモチーフを利用したrna結合性蛋白質の設計方法及びその利用
WO2014175284A1 (ja) * 2013-04-22 2014-10-30 国立大学法人九州大学 Ｐｐｒモチーフを利用したｄｎａ結合性タンパク質およびその利用
WO2018030488A1 (ja) * 2016-08-10 2018-02-15 和光純薬工業株式会社 Ｐｐｒモチーフを利用したｄｎａ結合性タンパク質およびその利用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2017275184B2 (en) 2016-06-03 2021-05-06 Kyushu University, National University Corporation Fusion protein for improving protein expression from target mRNA
EP3802566A4 (en) * 2018-06-06 2022-03-09 The University Of Western Australia PROTEINS AND THEIR USE IN NUCLEOTIDE BINDING

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030126646A1 (en) * 2001-07-12 2003-07-03 Brown Gregory G. Nuclear fertility restorer genes and methods of use in plants
WO2011111829A1 (ja) * 2010-03-11 2011-09-15 国立大学法人九州大学 Pprモチーフを利用したrna結合性蛋白質の改変方法
WO2013058404A1 (ja) * 2011-10-21 2013-04-25 国立大学法人九州大学 Pprモチーフを利用したrna結合性蛋白質の設計方法及びその利用
WO2014175284A1 (ja) * 2013-04-22 2014-10-30 国立大学法人九州大学 Ｐｐｒモチーフを利用したｄｎａ結合性タンパク質およびその利用
WO2018030488A1 (ja) * 2016-08-10 2018-02-15 和光純薬工業株式会社 Ｐｐｒモチーフを利用したｄｎａ結合性タンパク質およびその利用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CUICUI, S. et al, "Structural basis for specific single-stranded RNA recognition by designer pentatricopeptide repeat proteins", Nature Communications, 2016, vol. 7, no. 1, pages 1-8 *
YAN, J. et al, "Delineation of pentatricopeptide repeat codes for target RNA prediction", Nucleic Acids Research, February 2019, vol. 47, no. 7, pages 3728-3738 *

Also Published As

Publication number Publication date
EP3978513A1 (en) 2022-04-06
EP3978513A4 (en) 2023-06-28
AU2025204992A1 (en) 2025-07-24
JP2025107351A (ja) 2025-07-17
CN114729017A (zh) 2022-07-08
JP7491515B2 (ja) 2024-05-28
JP2023145672A (ja) 2023-10-11
JP7683865B2 (ja) 2025-05-27
KR20220023985A (ko) 2022-03-03
AU2020283177A1 (en) 2022-01-06
CA3142299A1 (en) 2020-12-03
BR112021023878A2 (pt) 2022-01-25
SG11202113055UA (en) 2021-12-30
WO2020241876A1 (ja) 2020-12-03
IL288426A (en) 2022-01-01
NZ783345A (en) 2025-06-27
JPWO2020241876A1 (https=) 2020-12-03
US20220289795A1 (en) 2022-09-15

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Free format text: THE NATURE OF THE AMENDMENT IS: AMEND THE INVENTION TITLE TO READ EFFICIENT METHOD FOR PREPARING PPR PROTEIN AND USE OF THE SAME

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