AU2020283177B2 - Efficient method for preparing ppr protein and use of the same - Google Patents

Efficient method for preparing ppr protein and use of the same Download PDF

Info

Publication number
AU2020283177B2
AU2020283177B2 AU2020283177A AU2020283177A AU2020283177B2 AU 2020283177 B2 AU2020283177 B2 AU 2020283177B2 AU 2020283177 A AU2020283177 A AU 2020283177A AU 2020283177 A AU2020283177 A AU 2020283177A AU 2020283177 B2 AU2020283177 B2 AU 2020283177B2
Authority
AU
Australia
Prior art keywords
substitution
amino acid
seq
ppr
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2020283177A
Other languages
English (en)
Other versions
AU2020283177A1 (en
Inventor
Takahiro Nakamura
Yusuke Yagi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Editforce Inc
Kyushu University NUC
Original Assignee
Editforce Inc
Kyushu University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Editforce Inc, Kyushu University NUC filed Critical Editforce Inc
Publication of AU2020283177A1 publication Critical patent/AU2020283177A1/en
Application granted granted Critical
Publication of AU2020283177B2 publication Critical patent/AU2020283177B2/en
Priority to AU2025204992A priority Critical patent/AU2025204992A1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/85—Fusion polypeptide containing an RNA binding domain

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AU2020283177A 2019-05-29 2020-05-29 Efficient method for preparing ppr protein and use of the same Active AU2020283177B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2025204992A AU2025204992A1 (en) 2019-05-29 2025-06-30 Efficient method for preparing ppr protein and use of the same

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019-100551 2019-05-29
JP2019100551 2019-05-29
PCT/JP2020/021472 WO2020241876A1 (ja) 2019-05-29 2020-05-29 効率的なｐｐｒタンパク質の作製方法及びその利用

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2025204992A Division AU2025204992A1 (en) 2019-05-29 2025-06-30 Efficient method for preparing ppr protein and use of the same

Publications (2)

Publication Number Publication Date
AU2020283177A1 AU2020283177A1 (en) 2022-01-06
AU2020283177B2 true AU2020283177B2 (en) 2025-05-08

Family

ID=73553200

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2020283177A Active AU2020283177B2 (en) 2019-05-29 2020-05-29 Efficient method for preparing ppr protein and use of the same
AU2025204992A Pending AU2025204992A1 (en) 2019-05-29 2025-06-30 Efficient method for preparing ppr protein and use of the same

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2025204992A Pending AU2025204992A1 (en) 2019-05-29 2025-06-30 Efficient method for preparing ppr protein and use of the same

Country Status (11)

Country Link
US (1) US20220289795A1 (https=)
EP (1) EP3978513A4 (https=)
JP (3) JP7491515B2 (https=)
KR (1) KR20220023985A (https=)
CN (1) CN114729017A (https=)
AU (2) AU2020283177B2 (https=)
BR (1) BR112021023878A2 (https=)
CA (1) CA3142299A1 (https=)
IL (1) IL288426A (https=)
SG (1) SG11202113055UA (https=)
WO (1) WO2020241876A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3977849A4 (en) * 2019-05-29 2023-06-28 Editforce, Inc. Ppr protein with less aggregation and use thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030126646A1 (en) * 2001-07-12 2003-07-03 Brown Gregory G. Nuclear fertility restorer genes and methods of use in plants
WO2011111829A1 (ja) * 2010-03-11 2011-09-15 国立大学法人九州大学 Pprモチーフを利用したrna結合性蛋白質の改変方法
WO2013058404A1 (ja) * 2011-10-21 2013-04-25 国立大学法人九州大学 Pprモチーフを利用したrna結合性蛋白質の設計方法及びその利用
WO2014175284A1 (ja) * 2013-04-22 2014-10-30 国立大学法人九州大学 Ｐｐｒモチーフを利用したｄｎａ結合性タンパク質およびその利用
WO2018030488A1 (ja) * 2016-08-10 2018-02-15 和光純薬工業株式会社 Ｐｐｒモチーフを利用したｄｎａ結合性タンパク質およびその利用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017209122A1 (ja) 2016-06-03 2017-12-07 国立大学法人九州大学 標的ｍＲＮＡからのタンパク質発現量を向上させるための融合タンパク質
US12441770B2 (en) * 2018-06-06 2025-10-14 The University Of Western Australia Proteins and their use for nucleotide binding

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030126646A1 (en) * 2001-07-12 2003-07-03 Brown Gregory G. Nuclear fertility restorer genes and methods of use in plants
WO2011111829A1 (ja) * 2010-03-11 2011-09-15 国立大学法人九州大学 Pprモチーフを利用したrna結合性蛋白質の改変方法
WO2013058404A1 (ja) * 2011-10-21 2013-04-25 国立大学法人九州大学 Pprモチーフを利用したrna結合性蛋白質の設計方法及びその利用
WO2014175284A1 (ja) * 2013-04-22 2014-10-30 国立大学法人九州大学 Ｐｐｒモチーフを利用したｄｎａ結合性タンパク質およびその利用
WO2018030488A1 (ja) * 2016-08-10 2018-02-15 和光純薬工業株式会社 Ｐｐｒモチーフを利用したｄｎａ結合性タンパク質およびその利用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CUICUI, S. et al, "Structural basis for specific single-stranded RNA recognition by designer pentatricopeptide repeat proteins", Nature Communications, 2016, vol. 7, no. 1, pages 1-8 *
YAN, J. et al, "Delineation of pentatricopeptide repeat codes for target RNA prediction", Nucleic Acids Research, February 2019, vol. 47, no. 7, pages 3728-3738 *

Also Published As

Publication number Publication date
JP2025107351A (ja) 2025-07-17
AU2020283177A1 (en) 2022-01-06
JP7683865B2 (ja) 2025-05-27
JP7491515B2 (ja) 2024-05-28
JPWO2020241876A1 (https=) 2020-12-03
KR20220023985A (ko) 2022-03-03
WO2020241876A1 (ja) 2020-12-03
NZ783345A (en) 2025-06-27
AU2025204992A1 (en) 2025-07-24
SG11202113055UA (en) 2021-12-30
EP3978513A1 (en) 2022-04-06
US20220289795A1 (en) 2022-09-15
EP3978513A4 (en) 2023-06-28
CN114729017A (zh) 2022-07-08
IL288426A (en) 2022-01-01
BR112021023878A2 (pt) 2022-01-25
CA3142299A1 (en) 2020-12-03
JP2023145672A (ja) 2023-10-11

Similar Documents

Publication Publication Date Title
US20100129807A1 (en) Stabilization of cyclic peptide structures
WO2023143150A1 (zh) 新型Cas酶和系统以及应用
JP2025107351A (ja) 効率的なｐｐｒタンパク質の作製方法及びその利用
Nguyen et al. Purification of an eight subunit RNA polymerase I complex in Trypanosoma brucei
JP2025181985A (ja) 凝集の少ないｐｐｒタンパク質及びその利用
Jackobel et al. DNA binding preferences of S. cerevisiae RNA polymerase I Core Factor reveal a preference for the GC-minor groove and a conserved binding mechanism
EP2554672B1 (en) Nucleic acid structure, method for producing complex using same, and screening method
RU2833901C2 (ru) Эффективный способ получения белка ppr и его использование
HK40068942A (en) Efficient ppr protein production method and use thereof
Chatterjee et al. RNA promotes mitochondrial import of F1-ATP synthase subunit alpha (ATP5A1)
CN115261359A (zh) 一种新型crispr酶和系统以及应用
HK40070719A (en) Efficient ppr protein production method and use thereof
HK40070059A (en) Ppr protein with less aggregation and use thereof
Sharma et al. Modulating TRAP-mediated transcription termination by AT during transcription of the leader region of the Bacillus subtilis trp operon
Lansink et al. Specialized RNA decay fine-tunes monogenic antigen expression in Trypanosoma brucei
Morikawa et al. A conserved fungal transcription factor domain drives protein-protein interactions and necrotrophic effector-mediated virulence in Parastagonospora nodorum
CN119859623A (zh) 一种调节cGAS和DNA的液-液相分离的方法
Altmannova et al. Mer3 helicase protects early crossover intermediates from STR complex disassembly during meiosis
Winzeler Mutation of I562 and Y565 in the CC2 Domain of Atg11 May Disrupt Protein Folding
Ziegler Engineering and Characterization of RNA-binding LOV photoreceptors
Barreto A genetic screen to isolate “lariat” peptide inhibitors of protein function

Legal Events

Date Code Title Description
DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS: AMEND THE INVENTION TITLE TO READ EFFICIENT METHOD FOR PREPARING PPR PROTEIN AND USE OF THE SAME

FGA Letters patent sealed or granted (standard patent)