SG11202113055UA - Efficient method for preparing ppr protein and use of the same - Google Patents

Efficient method for preparing ppr protein and use of the same

Info

Publication number
SG11202113055UA
SG11202113055UA SG11202113055UA SG11202113055UA SG11202113055UA SG 11202113055U A SG11202113055U A SG 11202113055UA SG 11202113055U A SG11202113055U A SG 11202113055UA SG 11202113055U A SG11202113055U A SG 11202113055UA SG 11202113055U A SG11202113055U A SG 11202113055UA
Authority
SG
Singapore
Prior art keywords
substitution
amino acid
ppr
sequence
amino acids
Prior art date
Application number
SG11202113055UA
Other languages
English (en)
Inventor
Yusuke Yagi
Takahiro Nakamura
Original Assignee
Editforce Inc
Univ Kyushu Nat Univ Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Editforce Inc, Univ Kyushu Nat Univ Corp filed Critical Editforce Inc
Publication of SG11202113055UA publication Critical patent/SG11202113055UA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/85—Fusion polypeptide containing an RNA binding domain

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)
SG11202113055UA 2019-05-29 2020-05-29 Efficient method for preparing ppr protein and use of the same SG11202113055UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2019100551 2019-05-29
PCT/JP2020/021472 WO2020241876A1 (ja) 2019-05-29 2020-05-29 効率的なｐｐｒタンパク質の作製方法及びその利用

Publications (1)

Publication Number Publication Date
SG11202113055UA true SG11202113055UA (en) 2021-12-30

Family

ID=73553200

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202113055UA SG11202113055UA (en) 2019-05-29 2020-05-29 Efficient method for preparing ppr protein and use of the same

Country Status (11)

Country Link
US (1) US20220289795A1 (https=)
EP (1) EP3978513A4 (https=)
JP (3) JP7491515B2 (https=)
KR (1) KR20220023985A (https=)
CN (1) CN114729017A (https=)
AU (2) AU2020283177B2 (https=)
BR (1) BR112021023878A2 (https=)
CA (1) CA3142299A1 (https=)
IL (1) IL288426A (https=)
SG (1) SG11202113055UA (https=)
WO (1) WO2020241876A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202113057XA (en) * 2019-05-29 2021-12-30 Editforce Inc Ppr protein causing less aggregation and use of the same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2452633A1 (en) * 2001-07-12 2003-01-23 Mcgill University Nuclear fertility restorer genes and methods of use in plants
JP2013128413A (ja) * 2010-03-11 2013-07-04 Kyushu Univ Ｐｐｒモチーフを利用したｒｎａ結合性蛋白質の改変方法
DK2784157T3 (da) 2011-10-21 2019-10-21 Univ Kyushu Nat Univ Corp Designfremgangsmåde til et rna-bindende protein under anvendelse af ppr-motiv og anvendelse deraf
AU2014258386B2 (en) * 2013-04-22 2018-10-25 Hiroshima University DNA binding protein using PPR motif, and use thereof
AU2017275184B2 (en) 2016-06-03 2021-05-06 Kyushu University, National University Corporation Fusion protein for improving protein expression from target mRNA
EP3498726A4 (en) * 2016-08-10 2020-03-25 Fujifilm Wako Pure Chemical Corporation DNA BINDING PROTEIN USING A PPR PATTERN AND USE OF SAID DNA BINDING PROTEIN
EP3802566A4 (en) * 2018-06-06 2022-03-09 The University Of Western Australia PROTEINS AND THEIR USE IN NUCLEOTIDE BINDING

Also Published As

Publication number Publication date
EP3978513A1 (en) 2022-04-06
EP3978513A4 (en) 2023-06-28
AU2025204992A1 (en) 2025-07-24
JP2025107351A (ja) 2025-07-17
CN114729017A (zh) 2022-07-08
JP7491515B2 (ja) 2024-05-28
JP2023145672A (ja) 2023-10-11
JP7683865B2 (ja) 2025-05-27
KR20220023985A (ko) 2022-03-03
AU2020283177B2 (en) 2025-05-08
AU2020283177A1 (en) 2022-01-06
CA3142299A1 (en) 2020-12-03
BR112021023878A2 (pt) 2022-01-25
WO2020241876A1 (ja) 2020-12-03
IL288426A (en) 2022-01-01
NZ783345A (en) 2025-06-27
JPWO2020241876A1 (https=) 2020-12-03
US20220289795A1 (en) 2022-09-15

Similar Documents

Publication Publication Date Title
Grozdanov et al. SHQ1 is required prior to NAF1 for assembly of H/ACA small nucleolar and telomerase RNPs
JP4262778B2 (ja) 生体分子相互作用を検出するためのタンパク質断片相補性アッセイ
JP2021000086A (ja) Ｒｎａガイド型核酸結合タンパク質を使用する分子間近接性検出用の方法および試薬
Ibraheem et al. A bacteria colony-based screen for optimal linker combinations in genetically encoded biosensors
Bressendorff et al. Importance of an N-terminal structural switch in the distinction between small RNA-bound and free ARGONAUTE
JP2025107351A (ja) 効率的なｐｐｒタンパク質の作製方法及びその利用
JP2025181985A (ja) 凝集の少ないｐｐｒタンパク質及びその利用
Seidel et al. The Aspergillus nidulans kinesin-3 tail is necessary and sufficient to recognize modified microtubules
Chatterjee et al. RNA promotes mitochondrial import of F1-ATP synthase subunit alpha (ATP5A1)
JP2020531572A (ja) メチル化により修飾されたｄｎａ塩基を特異的に認識するｔａｌｅ ｒｖｄおよびその使用
RU2833901C2 (ru) Эффективный способ получения белка ppr и его использование
HK40068942A (en) Efficient ppr protein production method and use thereof
Hagen et al. Characterization and inhibition of AF10‐mediated interaction
HK40070719A (en) Efficient ppr protein production method and use thereof
HK40070059A (en) Ppr protein with less aggregation and use thereof
Chen et al. AP profiling resolves co-translational folding pathway and chaperone interactions in vivo
HK40061571A (en) Ppr protein with less aggregation and use thereof
Altmannova et al. Mer3 helicase protects early crossover intermediates from STR complex disassembly during meiosis
Barreto A genetic screen to isolate “lariat” peptide inhibitors of protein function
BR112021023937B1 (pt) Polipeptídeo, uso do polipeptídeo, proteína capaz de se ligar a um ácido nucleico alvo, proteína de fusão, métodos para reduzir a agregração de uma proteína ppr contendo o motivo ppr, para manipular um ácido nucleico, para detectar um ácido nucleico e para produzir um microrganismo, ácido nucleico, vetor, e, célula
Traczyk G-quadruplex recognition by the yeast telomere binding protein Rap1