JP7491515B2 - 効率的なｐｐｒタンパク質の作製方法及びその利用 - Google Patents

効率的なｐｐｒタンパク質の作製方法及びその利用 Download PDF

Info

Publication number
JP7491515B2
JP7491515B2 JP2021521907A JP2021521907A JP7491515B2 JP 7491515 B2 JP7491515 B2 JP 7491515B2 JP 2021521907 A JP2021521907 A JP 2021521907A JP 2021521907 A JP2021521907 A JP 2021521907A JP 7491515 B2 JP7491515 B2 JP 7491515B2
Authority
JP
Japan
Prior art keywords
amino acid
sequence
substitution
seq
ppr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2021521907A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2020241876A1 (https=
JPWO2020241876A5 (https=
Inventor
祐介 八木
崇裕 中村
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EDITFORCE, INC.
Kyushu University NUC
Original Assignee
EDITFORCE, INC.
Kyushu University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EDITFORCE, INC., Kyushu University NUC filed Critical EDITFORCE, INC.
Publication of JPWO2020241876A1 publication Critical patent/JPWO2020241876A1/ja
Publication of JPWO2020241876A5 publication Critical patent/JPWO2020241876A5/ja
Priority to JP2023126196A priority Critical patent/JP7683865B2/ja
Application granted granted Critical
Publication of JP7491515B2 publication Critical patent/JP7491515B2/ja
Priority to JP2025076735A priority patent/JP2025107351A/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/85—Fusion polypeptide containing an RNA binding domain

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
JP2021521907A 2019-05-29 2020-05-29 効率的なｐｐｒタンパク質の作製方法及びその利用 Active JP7491515B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2023126196A JP7683865B2 (ja) 2019-05-29 2023-08-02 効率的なｐｐｒタンパク質の作製方法及びその利用
JP2025076735A JP2025107351A (ja) 2019-05-29 2025-05-02 効率的なｐｐｒタンパク質の作製方法及びその利用

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019100551 2019-05-29
JP2019100551 2019-05-29
PCT/JP2020/021472 WO2020241876A1 (ja) 2019-05-29 2020-05-29 効率的なｐｐｒタンパク質の作製方法及びその利用

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2023126196A Division JP7683865B2 (ja) 2019-05-29 2023-08-02 効率的なｐｐｒタンパク質の作製方法及びその利用

Publications (3)

Publication Number Publication Date
JPWO2020241876A1 JPWO2020241876A1 (https=) 2020-12-03
JPWO2020241876A5 JPWO2020241876A5 (https=) 2022-06-30
JP7491515B2 true JP7491515B2 (ja) 2024-05-28

Family

ID=73553200

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2021521907A Active JP7491515B2 (ja) 2019-05-29 2020-05-29 効率的なｐｐｒタンパク質の作製方法及びその利用
JP2023126196A Active JP7683865B2 (ja) 2019-05-29 2023-08-02 効率的なｐｐｒタンパク質の作製方法及びその利用
JP2025076735A Pending JP2025107351A (ja) 2019-05-29 2025-05-02 効率的なｐｐｒタンパク質の作製方法及びその利用

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2023126196A Active JP7683865B2 (ja) 2019-05-29 2023-08-02 効率的なｐｐｒタンパク質の作製方法及びその利用
JP2025076735A Pending JP2025107351A (ja) 2019-05-29 2025-05-02 効率的なｐｐｒタンパク質の作製方法及びその利用

Country Status (11)

Country Link
US (1) US20220289795A1 (https=)
EP (1) EP3978513A4 (https=)
JP (3) JP7491515B2 (https=)
KR (1) KR20220023985A (https=)
CN (1) CN114729017A (https=)
AU (2) AU2020283177B2 (https=)
BR (1) BR112021023878A2 (https=)
CA (1) CA3142299A1 (https=)
IL (1) IL288426A (https=)
SG (1) SG11202113055UA (https=)
WO (1) WO2020241876A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3977849A4 (en) * 2019-05-29 2023-06-28 Editforce, Inc. Ppr protein with less aggregation and use thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013058404A1 (ja) 2011-10-21 2013-04-25 国立大学法人九州大学 Pprモチーフを利用したrna結合性蛋白質の設計方法及びその利用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002322469B2 (en) * 2001-07-12 2008-05-22 Dna Landmarks, Inc. Nuclear fertility restorer genes and methods of use in plants
JP2013128413A (ja) * 2010-03-11 2013-07-04 Kyushu Univ Ｐｐｒモチーフを利用したｒｎａ結合性蛋白質の改変方法
KR102893770B1 (ko) * 2013-04-22 2025-11-28 고쿠리쓰다이가쿠호진 규슈다이가쿠 피피알 단백질을 이용하는 dna 결합성 단백질 및 그의 이용
WO2017209122A1 (ja) 2016-06-03 2017-12-07 国立大学法人九州大学 標的ｍＲＮＡからのタンパク質発現量を向上させるための融合タンパク質
JPWO2018030488A1 (ja) * 2016-08-10 2019-06-13 富士フイルム和光純薬株式会社 Ｐｐｒモチーフを利用したｄｎａ結合性タンパク質およびその利用
US12441770B2 (en) * 2018-06-06 2025-10-14 The University Of Western Australia Proteins and their use for nucleotide binding

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013058404A1 (ja) 2011-10-21 2013-04-25 国立大学法人九州大学 Pprモチーフを利用したrna結合性蛋白質の設計方法及びその利用

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ＮＡＴＵＲＥ ＣＯＭＭＵＮＩＣＡＴＩＯＮＳ， ２０１４， Ｖｏｌ．５， ５７２９， ｐ．１－９
Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓｅａｒｃｈ， １１ Ｆｅｂｕｒｕａｒｙ ２０１９， Ｖｏｌ．４７， ｐ．３７２８－３７３８
Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓｅａｒｃｈ， ２０１８， Ｖｏｌ．４６， ｐ．２６１３－２６２３

Also Published As

Publication number Publication date
JP2025107351A (ja) 2025-07-17
AU2020283177A1 (en) 2022-01-06
JP7683865B2 (ja) 2025-05-27
JPWO2020241876A1 (https=) 2020-12-03
KR20220023985A (ko) 2022-03-03
WO2020241876A1 (ja) 2020-12-03
NZ783345A (en) 2025-06-27
AU2025204992A1 (en) 2025-07-24
SG11202113055UA (en) 2021-12-30
EP3978513A1 (en) 2022-04-06
US20220289795A1 (en) 2022-09-15
EP3978513A4 (en) 2023-06-28
CN114729017A (zh) 2022-07-08
IL288426A (en) 2022-01-01
AU2020283177B2 (en) 2025-05-08
BR112021023878A2 (pt) 2022-01-25
CA3142299A1 (en) 2020-12-03
JP2023145672A (ja) 2023-10-11

Similar Documents

Publication Publication Date Title
Wood et al. Intein applications: from protein purification and labeling to metabolic control methods
JP2025107351A (ja) 効率的なｐｐｒタンパク質の作製方法及びその利用
Bressendorff et al. Importance of an N-terminal structural switch in the distinction between small RNA-bound and free ARGONAUTE
JP2025181985A (ja) 凝集の少ないｐｐｒタンパク質及びその利用
EP2554672B1 (en) Nucleic acid structure, method for producing complex using same, and screening method
RU2833901C2 (ru) Эффективный способ получения белка ppr и его использование
Chatterjee et al. RNA promotes mitochondrial import of F1-ATP synthase subunit alpha (ATP5A1)
HK40070719A (en) Efficient ppr protein production method and use thereof
HK40068942A (en) Efficient ppr protein production method and use thereof
JP2017530726A (ja) 抗体様タンパク質
HK40061571A (en) Ppr protein with less aggregation and use thereof
JP5936145B2 (ja) ペプチド抗体のスクリーニングに使用する核酸構築物およびそれを用いたスクリーニング方法
Barreto A genetic screen to isolate “lariat” peptide inhibitors of protein function
HK1180010B (en) Nucleic acid structure, method for producing complex using same, and screening method
WO2015133611A1 (ja) 標的タンパク質の量の低減のためのポリペプチド、単離された核酸、組み換えベクター、及び形質転換体

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20211119

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220606

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20220606

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20220606

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20220719

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220920

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20221129

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20230130

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230303

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20230502

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230802

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230914

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20230914

A912 Re-examination (zenchi) completed and case transferred to appeal board

Free format text: JAPANESE INTERMEDIATE CODE: A912

Effective date: 20231013

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20240115

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20240229

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20240507

R150 Certificate of patent or registration of utility model

Ref document number: 7491515

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150