BR112021023878A2 - Motivo ppr, uso do motivo, proteína ppr, método para controlar o entrançamento de rna, método para detectar rna, proteína de fusão, ácido nucleico, vetor, célula, método para manipular rna, e, método para produzir um organismo - Google Patents

Motivo ppr, uso do motivo, proteína ppr, método para controlar o entrançamento de rna, método para detectar rna, proteína de fusão, ácido nucleico, vetor, célula, método para manipular rna, e, método para produzir um organismo

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Publication number
BR112021023878A2
BR112021023878A2 BR112021023878A BR112021023878A BR112021023878A2 BR 112021023878 A2 BR112021023878 A2 BR 112021023878A2 BR 112021023878 A BR112021023878 A BR 112021023878A BR 112021023878 A BR112021023878 A BR 112021023878A BR 112021023878 A2 BR112021023878 A2 BR 112021023878A2
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BR
Brazil
Prior art keywords
ppr
rna
motif
protein
organism
Prior art date
Application number
BR112021023878A
Other languages
English (en)
Inventor
Takahiro Nakamura
Yusuke Yagi
Original Assignee
Editforce Inc
Univ Kyushu Nat Univ Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Editforce Inc, Univ Kyushu Nat Univ Corp filed Critical Editforce Inc
Publication of BR112021023878A2 publication Critical patent/BR112021023878A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/85—Fusion polypeptide containing an RNA binding domain

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

motivo ppr, uso do motivo, proteína ppr, método para controlar o entrançamento de rna, método para detectar rna, proteína de fusão, ácido nucleico, vetor, célula, método para manipular rna, e, método para produzir um organismo. é provida uma proteína ppr com alto desempenho. essa proteína ppr tem mais motivos que estão ligados que os 7 a 14 motivos convencionais e se liga a uma longa sequência de base. os motivos ppr são representados por: (a-1) motivo ppr que compreende uma sequência de seq id no: 9 ou 401; (c-1) motivo ppr que compreende uma sequência de seq id no: 10; (g-1) motivo ppr que compreende uma sequência de seq id no: 11; e (u-1) motivo ppr que compreende uma sequência de seq id no: 12. esses motivos são úteis como motivos ppr para, na ordem citada, adenina, citosina, guanina e uracila em uma sequência de base alvo.
BR112021023878A 2019-05-29 2020-05-29 Motivo ppr, uso do motivo, proteína ppr, método para controlar o entrançamento de rna, método para detectar rna, proteína de fusão, ácido nucleico, vetor, célula, método para manipular rna, e, método para produzir um organismo BR112021023878A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2019100551 2019-05-29
PCT/JP2020/021472 WO2020241876A1 (ja) 2019-05-29 2020-05-29 効率的なｐｐｒタンパク質の作製方法及びその利用

Publications (1)

Publication Number Publication Date
BR112021023878A2 true BR112021023878A2 (pt) 2022-01-25

Family

ID=73553200

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021023878A BR112021023878A2 (pt) 2019-05-29 2020-05-29 Motivo ppr, uso do motivo, proteína ppr, método para controlar o entrançamento de rna, método para detectar rna, proteína de fusão, ácido nucleico, vetor, célula, método para manipular rna, e, método para produzir um organismo

Country Status (11)

Country Link
US (1) US20220289795A1 (pt)
EP (1) EP3978513A4 (pt)
JP (2) JP7491515B2 (pt)
KR (1) KR20220023985A (pt)
CN (1) CN114729017A (pt)
AU (1) AU2020283177A1 (pt)
BR (1) BR112021023878A2 (pt)
CA (1) CA3142299A1 (pt)
IL (1) IL288426A (pt)
SG (1) SG11202113055UA (pt)
WO (1) WO2020241876A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202113057XA (en) * 2019-05-29 2021-12-30 Editforce Inc Ppr protein causing less aggregation and use of the same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003006622A2 (en) * 2001-07-12 2003-01-23 Mcgill University Nuclear fertility restorer genes and methods of use in plants
JP2013128413A (ja) * 2010-03-11 2013-07-04 Kyushu Univ Ｐｐｒモチーフを利用したｒｎａ結合性蛋白質の改変方法
US9513283B2 (en) * 2011-10-21 2016-12-06 Kyushu University, National University Corporation Method for designing RNA binding protein utilizing PPR motif, and use thereof
KR20160007541A (ko) * 2013-04-22 2016-01-20 고쿠리쓰다이가쿠호진 규슈다이가쿠 피피알 모티프를 이용한 디앤에이 결합성 단백질 및 그의 이용
AU2017275184B2 (en) 2016-06-03 2021-05-06 Kyushu University, National University Corporation Fusion protein for improving protein expression from target mRNA
WO2018030488A1 (ja) * 2016-08-10 2018-02-15 和光純薬工業株式会社 Ｐｐｒモチーフを利用したｄｎａ結合性タンパク質およびその利用
US20230287060A1 (en) * 2018-06-06 2023-09-14 The University Of Western Australia Proteins and their use for nucleotide binding

Also Published As

Publication number Publication date
WO2020241876A1 (ja) 2020-12-03
JPWO2020241876A1 (pt) 2020-12-03
AU2020283177A1 (en) 2022-01-06
US20220289795A1 (en) 2022-09-15
KR20220023985A (ko) 2022-03-03
IL288426A (en) 2022-01-01
JP2023145672A (ja) 2023-10-11
CN114729017A (zh) 2022-07-08
EP3978513A4 (en) 2023-06-28
CA3142299A1 (en) 2020-12-03
SG11202113055UA (en) 2021-12-30
JP7491515B2 (ja) 2024-05-28
EP3978513A1 (en) 2022-04-06

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