KR20210071252A - Cosmetic composition for reducing hair loss - Google Patents

Abstract

The present invention relates to a cosmetic composition that can provide an improved hair loss reduction effect, including lava seawater, Gynostemma pentaphyllum leaf extracts, and hydrogen.

Description

Cosmetic composition for reducing hair loss

The present invention relates to a cosmetic composition that can provide an improved hair loss alleviation effect, including lava seawater, an extract of stony leaves, and hydrogen.

Hair loss refers to a state in which there is no hair in the area where hair should normally exist, and generally refers to the loss of hair on the scalp. Unlike soft hair, which is colorless and thin, hair loss causes cosmetic problems, so the market for preventing and treating hair loss is growing day by day. Accordingly, for alleviating hair loss, a composition for alleviating hair loss as described in the following patent documents has been developed.

<Patent Literature>

Patent No. 10-1727477 (registered on April 11, 2017) "Hair loss prevention and hair growth promoting composition"

However, the conventional composition for alleviating hair loss and the method of applying the same have a problem in that the hair loss alleviation effect is not satisfactory and the stability is not guaranteed.

The present invention is to solve the problems of the prior art described above,

It is an object of the present invention to provide a cosmetic composition having a hair loss alleviation effect, including lava seawater, dolex leaf extract and hydrogen.

In addition, an object of the present invention is to provide a cosmetic composition having an additional dandruff improvement effect by further including an extract for dandruff improvement.

The present invention is implemented by an embodiment having the following configuration in order to achieve the above object.

According to one embodiment of the present invention, the cosmetic composition according to the present invention contains lava seawater, dolex leaf extract, and hydrogen, and is characterized in that it provides a hair loss relief effect.

According to another embodiment of the present invention, the cosmetic composition according to the present invention induces phosphorylation of GSK-3β and reduces and stabilizes the degradation of β-catenin.

According to another embodiment of the present invention, in the cosmetic composition according to the present invention, the lava seawater has a hardness of 4000 to 6000, and 0.1 to 10 parts by weight of the lava seawater extract is used based on 100 parts by weight of the lava seawater, and the hydrogen is It is characterized in that it is directly dissolved in lava seawater and has a dissolved concentration of 0.7 to 1.8 ppm.

According to another embodiment of the present invention, the cosmetic composition according to the present invention further comprises an extract for dandruff relief, and the extract for dandruff relief is used in an amount of 0.1 to 10 parts by weight based on 100 parts by weight of lava seawater, Extracts for alleviating dandruff include 100 to 200 parts by weight of daffodil extract, 100 to 200 parts by weight of peppermint extract, 100 to 200 parts by weight of cotton export, 100 to 200 parts by weight of Orifolia extract, 100 to 200 parts by weight of extract of horsetail extract, Pengraecho 100 to 200 parts by weight of the extract, 100 to 200 parts by weight of the Pine extract, 100 to 200 parts by weight of the Citrus extract. And 100 to 200 parts by weight of the extract of the dandelion tree.

The present invention can obtain the following effects by the present embodiment above.

The present invention can provide a hair loss alleviation effect, including lava seawater, stone leaf extract and hydrogen.

In addition, the present invention may further provide an effect of improving dandruff by further including an extract for improving dandruff.

1 shows the results of Western Blot analysis for confirming the expression of hair-related proteins when human dermal papilla cells are treated with a cosmetic composition according to an embodiment of the present invention.

Hereinafter, the cosmetic composition for alleviating hair loss according to the present invention will be described in detail with reference to the accompanying drawings. Unless otherwise defined, all terms in this specification have the same general meaning as understood by those skilled in the art to which the present invention belongs, and in case of conflict with the meaning of the terms used in this specification, the According to the definition used in the specification. In addition, detailed descriptions of well-known functions and configurations that may unnecessarily obscure the gist of the present invention will be omitted. Throughout the specification, when a part "includes" a certain component, it means that other components may be further included, rather than excluding other components, unless otherwise stated.

The cosmetic composition according to an embodiment of the present invention contains lava seawater, dolex leaf extract, and hydrogen, and provides an effect of alleviating hair loss. The lava seawater refers to water from which seawater is naturally filtered by the volcanic bedrock and seeps into the ground underground, and lava seawater having a hardness (about the content of minerals such as magnesium and potassium) of 4000 to 6000 is preferably used. The dolecium is a perennial tendril plant of the dicotyledonous plant Gourdaceae family, and the dolectinaceae extract is dried and pulverized, then 10 times the weight of ethanol is added, stirred, extracted for 48 hours, filtered, concentrated under reduced pressure, and freeze-dried. It is formed, and the extract is preferably used in an amount of 0.1 to 10 parts by weight based on 100 parts by weight of lava seawater. The hydrogen is dissolved by being directly injected into water, and it is preferable that the dissolved concentration is 0.7 to 1.8 ppm. The cosmetic composition is mixed with lava seawater of a certain hardness, extract and hydrogen in a certain ratio, to induce phosphorylation of GSK-3β, reduce and stabilize the decomposition of β-catenin, etc. to provide a more improved hair loss mitigation effect. do.

The cosmetic composition according to another embodiment of the present invention further comprises an extract for alleviating dandruff, and the extract for dandruff relief is used in an amount of 0.1 to 10 parts by weight based on 100 parts by weight of lava seawater, and the extract for alleviating dandruff is 100 to 200 parts by weight of daffodil extract, 100 to 200 parts by weight of extract, 100 to 200 parts by weight of mint extract, 100 to 200 parts by weight of cotton export, 100 to 200 parts by weight of Orifolia extract, 100 to 200 parts by weight of extract of Malbaldrifolia, 100 to 200 parts by weight of extract of fenugreek , 100 to 200 parts by weight of the Pine extract, 100 to 200 parts by weight of the fenugreek extract, and 100 to 200 parts by weight of the Gymaceae extract. The daffodil is a monocotyledonous plant Liliaceae Narcissus, the hairy sagebrush is a perennial herb of the genus Lamiaceae and the genus Lamiaceae, the cotton is a dicotyledonous plant Malvaceae mallow and annual grass, and the Orifolia is a dicotyledonous plywood currency. It is a perennial herb of the plant order Lamiaceae, the horsetail is a dicotyledonous plant, a deciduous shrub of the Rosaceae family, the deciduous shrub of the family Rosaceae, and the pennywort is an evergreen vine of the monocotyledon family, the order Amonaceae, and the cypress is a perennial of the dicotyledon family of the callus plant of the order Hyonsengaceae It is a grass, and the perennial herb is an evergreen creeper plant of the Passionaceae family, and the persimmon tree is an evergreen broad-leaved small arboreous tree of the dicotyledonous plant, Novakaceae, Gamaceae.

The cosmetic composition of the present invention may further include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extract.

The water-soluble vitamin may be any type of vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, etc. and their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-phosphate magnesium salt, etc.) are also water-soluble vitamins that can be used in the present invention. are included in The water-soluble vitamin can be obtained by a conventional method such as a microbial transformation method, a purification method from a microbial culture, an enzymatic method or a chemical synthesis method.

The oil-soluble vitamins may be any as long as they can be formulated in cosmetics, but preferably include vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol). and their derivatives (ascorbine palmitate, ascorbine stearate, ascorbine dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinic acid, vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl ether, etc.) are also included in the oil-soluble vitamins used in the present invention. The oil-soluble vitamin can be obtained by a conventional method such as a microbial transformation method, a purification method from a microbial culture, an enzyme or a chemical synthesis method, and the like.

The polymer peptide may be any compound as long as it can be blended into a cosmetic. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like are mentioned. The polymer peptide can be purified and obtained by a conventional method such as purification from a microbial culture solution, an enzyme method, or a chemical synthesis method, or can be purified from natural products such as the dermis of pigs or cattle, silk fibers of silkworms, etc. .

The high-molecular polysaccharide may be any one as long as it can be blended into a cosmetic. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfuric acid or a salt thereof (sodium salt, etc.) is mentioned. For example, chondroitin sulfate or a salt thereof can be used after purification from mammals or fish.

The sphingolipid may be any as long as it can be blended into a cosmetic, and preferred examples include ceramide, phytosphingosine, and sphingoglycolipid. The sphingo lipid may be purified by a conventional method from mammals, fish, shellfish, yeast or plants, or may be obtained by chemical synthesis.

The seaweed extract may be any one as long as it can be formulated in cosmetics, but preferably brown algae extract, red algae extract, green algae extract, etc., and calrageenan purified from these seaweed extracts, arginic acid, sodium alginate , potassium alginate, etc. are also included in the seaweed extract used in the present invention. The said seaweed extract can be obtained by refine|purifying by a conventional method from seaweed.

The cosmetic composition of the present invention may contain other ingredients that are normally formulated in the cosmetic composition. For example, oils and fats, moisturizing agents, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, disinfectants, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation promoters, A cooling agent, an antiperspirant agent, purified water, etc. are mentioned.

Examples of the fats and oils include ester fats and oils, hydrocarbon oils, silicone oils, fluorine oils, animal fats, and vegetable oils. Examples of the ester-based oils and fats include glyceryl tri-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, stearate. Butyl acid, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adi Diisopropyl finate, isoalkyl neopentanoate, tri(caprylic acid, capric acid) glyceryl, trimethylolpropane tri2-ethylhexanoate, trimethylolpropane triisostearate, pentaellisly tetra2-ethylhexanoate Tol, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isosine laurate Tearyl, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isostearate isopropyl, 2-ethylhexanoate ceto Stearyl, 2-ethyl hexanoate stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, di(caprylic acid, capric acid) propylene glycol, dicaprylic acid propylene glycol, Neopentyl glycol dicapric acid, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostea octanoate Reel, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerol oleate ester, polyglycerol isostearate ester , triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, mal Diisostearyl acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, diox sebacate Thiyl, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytsteryl isostearate, phytsteryl oleate, 12-stearate Ester types, such as isocetyl alloyl hydroxy stearate, 12-stealoyl hydroxy stearate stearate, and 12-stealoyl hydroxy stearate isostearyl, etc. are mentioned. Examples of the hydrocarbon-based fats and oils include hydrocarbon-based fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybudene, microcrystalline wax, petrolatum. Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane/methylcetyloxysiloxane copolymer, dimethylsiloxane/methylstealloxysiloxane copolymer, Alkyl-modified silicone oil, amino-modified silicone oil, etc. are mentioned. As said fluorine-type fats and oils, perfluoro polyether etc. are mentioned. Examples of the animal or vegetable oils and fats include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, bird flower oil, soybean oil, corn oil, rapeseed oil, passerine oil, palm kernel oil, palm oil, castor oil, sunflower oil, grapes. Seed oil, cottonseed oil, palm oil, kukui nut oil, wheat germ oil, rice germ oil, shea butter, cranberry colostrum, marcadeumia nut oil, meadow home oil, egg yolk oil, beef tallow, horse oil, mink oil, orange rafi oil, jojoba and animal or plant oils and fats, such as oil, candelabra wax, carnauba wax, liquid lanolin, and hydrogenated castor oil.

Examples of the moisturizer include a water-soluble low molecular weight moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer. Examples of the water-soluble low molecular weight moisturizer include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), poly Propylene glycol (polymerization degree n=2 or more), polyglycerol B (polymerization degree n=2 or more), lactic acid, lactic acid salt, etc. are mentioned. Cholesterol, cholesterol ester, etc. are mentioned as said fat-soluble low molecular weight moisturizing agent. Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water-soluble chitin, chitosan, dextrin, and the like. can be heard Examples of the fat-soluble polymer include polyvinylpyrrolidone/eicosene copolymer, polyvinylpyrrolidone/hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and high molecular weight silicone.

As said emollient agent, long-chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl ester, etc. are mentioned.

Nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants, etc. are mentioned as said surfactant. Examples of the nonionic surfactant include self-emulsifying monostearic acid glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene), sorbitan fatty acid ester, POE sorbitan fatty acid ester , POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE castor oil, POEPOP (polyoxyethylene polyoxypropylene) copolymer, POEPOP alkyl ether, polyether-modified silicone, lauric acid alkanolamide , alkylamine oxide, hydrogenated soybean phospholipid, and the like. Examples of the anionic surfactant include fatty acid soap, alpha-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkyl amide sulfate, alkyl phosphate, POE alkyl phosphate, and alkyl. Amide phosphate, alkyloyl alkyl taurine salt, N-acyl amino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, alkyl sulfoacetate sodium, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester, etc. can Examples of the cationic surfactant include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, Benzalkonium chloride, diethylaminoethyl stearate, dimethylaminopropyl stearate, quaternary ammonium salt of lanolin derivative, etc. are mentioned. Examples of the amphoteric surfactant include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amidesulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amideamine type, etc. of amphoteric surfactants, and the like.

Examples of the organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium-coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, oxide. inorganic pigments such as aluminum, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine, chromium oxide, chromium hydroxide, calamine, and complexes thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene/styrene copolymer, and organic pigments such as silk powder, cellulose, CI pigment yellow and CI pigment orange, and composite pigments of these inorganic pigments and organic pigments.

Examples of the organic powder include metal soaps such as calcium stearate; alkyl phosphate metal salts such as sodium cetylate, zinc laurylate, and calcium laurylate; acylamino acid polyvalent metal salts such as calcium N-lauroyl-beta-alanine, zinc N-lauroyl-beta-alanine, and calcium N-lauroyl glycine; amidesulfonic acid polyvalent metal salts such as calcium N-lauroyl-taurine and calcium N-palmitoyl-taurine; N such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylizine, N-alpha-paritoylolnithine, N-alpha-lauroylarginine, N-alpha-hydrogenated beef fatty acid acylarginine, etc. -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, a divinylbenzene-styrene copolymer, ethylene tetrafluoride, etc. are mentioned.

Examples of the ultraviolet absorber include para-aminobenzoic acid, ethyl para-aminobenzoate, amyl para-aminobenzoate, octyl para-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylate, cinnamic acid. Benzyl, para-methoxycinnamic acid-2-ethoxyethyl, para-methoxycinnamic acid octyl, dipara-methoxycinnamic acid mono-2-ethylhexane glyceryl, para-methoxycinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture; Urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenonedisulfonate sodium, dihydroxybenzo Phenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)- 1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl) benzotriazole, etc. are mentioned.

Examples of the disinfectant include hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zincphyllithione, benzalkonium chloride, Photosensitizer No. 301, mononitroguaial sodium, undecyrenic acid, etc. are mentioned.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and ellisorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmalic acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogen phosphate, and the like.

As said alcohol, higher alcohols, such as cetyl alcohol, are mentioned.

In addition, blending components that may be added are not limited thereto, and any of the above components can be blended within a range that does not impair the object and effect of the present invention, but preferably 0.01 to 5% by weight relative to the total weight, more preferably 0.01 to 3% by weight.

The cosmetic composition of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Components included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the above compound as an active ingredient, for example, conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances and carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, emulsion, cream, foundation, lotion, cosmetic liquid, hair cosmetics, and the like. Specifically, the cosmetic composition of the present invention is a skin lotion, skin softener, moisture lotion, nourishing lotion, massage cream, nourishing cream, hand cream, moisture cream, essence, pack, soap, lotion, milk lotion, gel, ointment, patch, Includes cleansing foam, body cleanser, astringent, and spray formulations. When the formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether. When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan. When the formulation of the present invention is a suspension, as a carrier component, water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used. When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester and the like can be used.

Hereinafter, the present invention will be described in more detail through examples. However, these are only for describing the present invention in more detail, and the scope of the present invention is not limited thereto.

<Example 1> Preparation of samples

1. Preparation of samples for alleviating hair loss

(1) After drying and pulverizing the stone leaf, 10 times the weight of ethanol was added thereto, followed by stirring, extraction for 48 hours, filtration, concentration under reduced pressure, and freeze-drying to obtain a stone leaf extract (sample of stone leaf).

(2) Distilled water, lava seawater, dolex leaf extract, hydrogen, etc. were mixed in the ratio shown in Table 1 below to obtain hair loss samples 1 to 14. The lava seawater was extracted and treated in Gujwa-eup, Jeju-si, and the hardness was adjusted. For the dolex leaf extract, the dolex sample was used, and the hydrogen was directly injected into distilled water or lava seawater to dissolve.

Distilled water (parts by weight) Lava seawater (parts by weight) Stone samples (parts by weight) Hydrogen
(Dissolved concentration (ppm)) Hair loss sample 1 100 0 0 0 hair loss sample 2 100 0 2.5 0 hair loss sample 3 100 0 0 1.5 hair loss sample 4 100 0 2.5 1.5 hair loss sample 5 0 100 (hardness 5000) 0 0 hair loss sample 6 0 100 (hardness 5000) 2.5 0 Hair loss sample 7 0 100 (hardness 5000) 0 1.5 hair loss sample 8 0 100 (hardness 5000) 2.5 1.5 hair loss sample 9 0 100 (hardness 3000) 2.5 1.5 hair loss sample 10 0 100 (hardness 7000) 2.5 1.5 Hair loss sample 11 0 100 (hardness 5000) 1.5 1.5 Hair loss sample 12 0 100 (hardness 5000) 3.5 1.5 Hair loss sample 13 0 100 (hardness 5000) 2.5 0.7 Hair loss sample 14 0 100 (hardness 5000) 2.5 2.3

2. Preparation of samples for dandruff improvement

Dried and pulverized daffodils (root) 10g, wild peppermint (leaf) 10g, cotton (leaf) 10g, oleander (leaf) 10g, horsetail (leaf) 10g, pennywort (leaf) 10g, cypress (leaf) 10g, passionflower 10 g (leaf) and 10 g of dandelion tree (leaf) were mixed in 1 L of ethanol and stirred to extract for 48 hours, filtered, concentrated under reduced pressure, and freeze-dried to prepare a sample for improving dandruff.

<Example 2> Confirmation of hair loss alleviation effect

1. Confirmation of hair growth through mouse biopsy

(1) The back of a 7-week-old C57BL/6 mouse was removed, and samples 1 to 14 were applied in the same amount every day. After 25 days, the back skin was collected and stored in a formalin fixative. The mouse skin stored in the formalin fixative was made into a paraffin block, sectioned, and stained with hematoxylin and eosin, the number of hair follicles per unit area was checked under an optical microscope, and the number of hair follicles per unit area was checked with an optical microscope. indicated.

(2) Referring to Table 1, it can be seen that lava seawater, extract and hydrogen each increase the number of hair follicles, and when lava seawater of a certain hardness, extract and hydrogen are mixed in a certain ratio, the number of hair follicles increases even more. can be seen to increase.

Number of follicles/number of follicles when sample 1 is used hair loss sample
One hair loss sample
2 hair loss sample
3 hair loss sample
4 hair loss sample
5 hair loss sample
6 hair loss sample
7 One 1.30 1.27 1.53 1.23 1.57 1.60 hair loss sample
8 hair loss sample
9 hair loss sample
10 hair loss sample
11 hair loss sample
12 hair loss sample
13 hair loss sample
14 2.02 1.67 1.77 1.83 1.86 1.81 1.89

2. Confirmation of expression of hair-related proteins

(1) To confirm the expression of hair-related proteins in human dermal papilla cells, human dermal papilla cells were treated with DMEM culture medium containing hair loss sample 8, etc. for 24 hours. After washing the cells, the cells are lysed with lysis buffer, separated through electrophoresis (SDS-PAGE), transferred to a membrane, and the protein is blocked so that the antibody does not attach to the non-transferred part, and then the expression of the desired protein It was reacted with an antibody that can detect whether or not it was, visualized and quantified, and the results are shown in FIG. 1 . In Figure 1, 1 shows the result when only DMEM medium was used as a culture medium, 2 shows the result when a culture solution containing 100 uM minoxidil was used in DMEM medium, and 3 is DMEM medium 50% and lava seawater ( Hardness 5000) shows the results when a culture solution containing 50% is used, and 4 shows the results when a culture solution containing 50% of DMEM medium and 50% of the hair loss sample 8 is used in a volume ratio.

(2) β-catenin is a transcription factor that increases the expression of various genes (c-Myc, CyclinD1, Growth factor, etc.) that induce hair growth and cell proliferation. Under conditions of hair loss, β-catenin is GSK-3β In the condition that promotes hair growth, the Ser 9 site of GSK-3β is phosphorylated by the upper enzyme Akt, reducing GSK-3β activity, and β-catenin is stabilized to promote hair growth. 1, it can be seen that the cosmetic composition of the present invention induces phosphorylation of GSK-3β and reduces and stabilizes the degradation of β-catenin.

<Example 3> Confirmation of dandruff improvement effect

1. Confirmation of dandruff improvement effect through sebum suppression

(1) Excessive secretion of sebum corresponds to one cause of dandruff. Hereinafter, it will be confirmed that a sample for improving dandruff is applied to humans to reduce the secretion of sebum.

(2) A cosmetic composition for improving dandruff comprising the dandruff improvement sample, preservative 0.1wt%, glycerin 2wt%, fragrance 0.1wt%, and purified water remaining wt% was prepared. The cosmetic compositions 1 to 4 for improving dandruff include 0wt%, 0.5wt%, 1wt%, and 2wt% of wrinkle improvement samples, respectively.

(3) For 8 males in their 40s with excessive sebum secretion, after washing their foreheads, wipe the forehead with ethanol and dry them. After 2 hours, the detail tape was attached to the forehead and removed to confirm. After that, the forehead area was allowed to use the same amount of the cosmetic compositions 1 to 4 for improving dandruff for 4 weeks (wet the sheet with the cosmetic composition and attach the sheet to the forehead for a certain period of time), 4 weeks passed The next day, wash the face, wipe it with ethanol, dry it, and after 2 hours, attach the detail tape to the forehead and remove it to confirm. The relative reduction of sebum was confirmed by checking the detailed tapes before and after application of the cosmetic composition for dandruff improvement.

(4) As a result of the verification of sebum through the above experiment, it was confirmed that no skin irritation occurred even when the cosmetic composition for improving dandruff was used for 4 weeks, and the relative reduction of sebum before and after using the cosmetic composition for improving dandruff was confirmed. As a result, as a result of using the cosmetic composition 1 for improving dandruff, sebum increased slightly, but when the cosmetic compositions 2 to 4 for improving dandruff were used, a significant reduction in sebum was confirmed. In addition, it was confirmed that the sebum reduction was greater from 2 to 4 of the cosmetic composition for dandruff improvement.

2. Confirmation of dandruff improvement effect by alleviating skin irritation

(1) Skin damage due to skin irritation is one of the causes of dandruff. Hereinafter, it will be confirmed that a sample for improving dandruff is applied to a person to alleviate skin irritation.

(2) For 16 women in their twenties without skin diseases, the cosmetic composition for improving dandruff in which 5.0% of lactic acid, a skin irritant, was added as an irritant was used. After wiping the back of the hand with 70% ethanol and drying, the cosmetic composition for dandruff improvement with the irritant added is sealed and applied, and 24 hours later, the patch is removed, and the presence or absence of erythema and edema is observed. Table 3 shows. The absence of erythema and edema was evaluated as 0, and if there was erythema and edema, the ranking was evaluated in the order of mild erythema and edema. In the case of 1st priority, there is erythema and edema, but it means the weakest case.

(3) Referring to Table 3, it can be seen that no skin irritation occurs with the cosmetic composition 4 for improving dandruff, and the skin irritation is weaker as the samples 1 to 4 go, so the sample for improving dandruff is included when treating the skin with an irritant It can be seen that the used cosmetic composition relieves skin irritation.

sample evaluation value 1 (Cosmetic composition for dandruff improvement 1+lactic acid) 4th place 2 (Cosmetic composition for dandruff improvement 2+lactic acid) 3rd place 3 (Cosmetic composition for dandruff improvement 3+lactic acid) 2nd place 4 (cosmetics composition for dandruff improvement 4+lactic acid) 1st place 5 (Cosmetics composition for dandruff improvement 4) 0

<Example 4> Confirmation of hair loss and dandruff improvement through skin patch experiment

1. A cosmetic composition sample was prepared in the composition ratio of Table 4 below.

cosmetic composition sample content(%) hair loss sample 8 Samples for improving dandruff antiseptic glycerin Spices Purified water One 0 0 0.1 2 0.1 remaining amount 2 50 0 0.1 2 0.1 remaining amount 3 50 1.5 0.1 2 0.1 remaining amount 4 0 1.5 0.1 2 0.1 remaining amount

2. For 10 men suffering from alopecia, after applying the same amount of cosmetic composition samples 1 to 4 to the hair loss area every morning and evening for 8 weeks (applied in spray form), hair loss improved after 8 weeks sensory evaluation was performed. In addition, after applying the same amount of samples 1 to 4 of the cosmetic composition on the hair every morning and evening for 8 weeks (applied in the form of a spray) to 10 men with frequent dandruff, dandruff improvement after 8 weeks sensory evaluation was performed. Correspondence evaluation results are shown in Table 5, and in the sensory evaluation items, the improved condition was evaluated as 5 points, bad condition as 0 points, and 3 points means the condition was neither improved nor deteriorated.

3. Looking at Table 5, it can be seen that the cosmetic composition samples 2 and 3 relieve hair loss, and the cosmetic composition samples 3 and 4 relieve dandruff.

cosmetic composition sample hair loss relief dandruff relief One 3.1 2.9 2 3.9 3.1 3 4.3 4.0 4 3.5 4.1

In the above, the applicant has described preferred embodiments of the present invention, but these embodiments are only one embodiment that implements the technical idea of the present invention, and any changes or modifications as long as the technical idea of the present invention is implemented. should be construed as within the scope.

Claims (4)

A cosmetic composition, comprising lava seawater, dolex leaf extract, and hydrogen, to provide a hair loss relief effect. According to claim 1,
The cosmetic composition induces phosphorylation of GSK-3β and reduces and stabilizes the degradation of β-catenin. 3. The method of claim 2,
The lava seawater has a hardness of 4000 to 6000, and the extract is 0.1 to 10 parts by weight based on 100 parts by weight of the lava seawater, and the hydrogen is directly dissolved in the lava seawater, characterized in that the dissolved concentration is 0.7 to 1.8ppm cosmetic composition. According to claim 1,
The cosmetic composition further comprises an extract for dandruff relief,
The extract for dandruff relief is used in 0.1 to 10 parts by weight based on 100 parts by weight of lava seawater
The extract for alleviating dandruff is 100 to 200 parts by weight of daffodil extract, 100 to 200 parts by weight of sagebrush extract, 100 to 200 parts by weight of cotton export, 100 to 200 parts by weight of Orifolia extract, 100 to 200 parts by weight of extract of horsetail, A cosmetic composition comprising 100 to 200 parts by weight of Pengrae extract, 100 to 200 parts by weight of Pine extract, 100 to 200 parts by weight of fenugreek extract, and 100 to 200 parts by weight of extract of Gumtang tree.

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