KR100982351B1 - Composition for promoting hair growth comprising magma seawater - Google Patents

Abstract

The present invention relates to a composition for promoting hair growth or preventing hair loss comprising lava seawater demineralized mineral water or concentrated mineral water as an active ingredient, and to a hair growth preparation containing natural extract extracted from lava seawater demineralized mineral water as an active ingredient.

According to the present invention, the lava seawater demineralized mineral water or concentrated mineral water may be usefully used as a hair growth agent composition or as an additive of an existing hair growth agent, and the lava seawater desalted mineral water or concentrated mineral water is used as an extraction solvent to promote hair growth. Or by extracting natural products that have been found to prevent and improve hair loss can be used to promote hair growth, hair loss prevention and hair improvement.

Lava seawater, lava seawater demineralized mineral water, lava seawater concentrated mineral water, hair growth (Hair Growth) effect, rat vibrissa hair follicle separation culture, 5 alpha-reductase, dermal papilla cell growth effect , Cytotoxicity

Description

Composition for promoting hair growth comprising magma seawater

The present invention relates to a hair growth agent composition containing lava seawater demineralized mineral water, and more specifically, natural extract extracted with lava seawater demineralized mineral water or lava seawater demineralized mineral water effective for promoting hair growth and preventing hair loss. It relates to a hair growth composition containing.

In the human body, hair grows in hair follicles on the skin, and hair follicles grow in the inner root sheath (IRS), outer root sheath (ORS), and hair. It is a complex organ composed of a hair shaft, hair matrix, and so on, and it is characterized by continuous cycles of anagen, catagen, and telogen throughout its life (Paus et al. , J Invest Dermatol. 113, pp 523-532, 1999). In particular, dermal papilla cells, which form hair follicles, play an important role in hair growth, and the substrate surrounding dermal papilla during the growth of hair follicles. Proliferation of constituent cells of the hair follicles, including keratinocytes of the cells (Oliver RF., J Embryol Exp Morphol. 15, pp331-347, 1996; Jahoda et al., Nature. 311, pp560-562, 1984; Paus et al. , J Invest Dermatol. 113, pp 523-532, 1999). In the degenerative phase of hair follicles, hair follicle growth stops, apoptosis, condensation of dermal papilla and reconstitution of extracellular matrix occur, and the hair follicles are prepared to enter the growth phase again. In the off-season, only permanent parts, including bluge regions, remain (Soma et al., J Invest Dermatol. 6, pp948-954, 1998; Jahoda et al., Nature. 311, pp560-562). , 1984; Paus et al., J Invest Dermatol. 113, pp 523-532, 1999).

Hair loss refers to the increase in hair growth in degenerative or resting hair follicles during the growth cycle of hair follicles, leading to an increase in hair falling out of hair follicles. Doing. The causes of such alopecia have been discussed, such as poor blood circulation, excessive male hormone action, excessive sebum secretion, scalp hypofunction caused by peroxides and bacteria, genetic factors, aging, stress, etc. The cause is unknown. In general, the most typical form of hair loss is androgenetic alopecia, which manifests as an excess of male hormone action, and is dependent on androgen (androgen), a male hormone (Paus et al., J Invest Dermaltol. 113, pp523-532, 1999). There are testosterone and dihydrotestosterone (DHT), which are known to have more dihydrotestosterone in alopecia patients (Van Neste et al., Br J Dermatol. 143 (3), pp804-810). , 2000). Testosterone is converted to dihydrotestosterone by 5 alpha-reductase, and this conversion is performed by 5 alpha-reductases of Type 2 of the two types of 5 alpha-reductases, Type 1 and Type 2. It has been reported that progressive diminishing dermal papilla and hair follicles is caused by converted dihydrotestosterone (Whiting D., J Am Acad Dermatol. 28, pp755-763, 1993; Singlair R., Br Med J. 317, pp865 -886, 1998).

The most widely used in the treatment and prevention of such alopecia is the upoxid (minoxidil) -containing agent of Upjohn Co., Ltd. approved by the US FDA and recently, Merck Co., Ltd. recently inhibited the type 2 5-alpha-reductase inhibitory effect. Propecia, the main ingredient of which is finasteride, has been released and used. In addition, studies based on herbal medicine are also underway, and the hair-repellent composition containing a topical coating hair regrowth agent (Korea Patent Registration No. 10-0476791) prepared by mixing extracts of mulberry leaves, baekbaek leaf, black sesame seeds, seaweed and seaweed. And a method for preparing the same (Korean Patent No. 10-0348155), a hair-growth composition consisting of extracts of Baekdusan, Daesan, and Gold having a fungicidal function of Demodex (Korean Patent No. 10-0487275). However, the clinical effects of these hair regrowths or hair restorers are not so excellent, and there are many studies to search for hair repellents that are more effective and have less side effects than minoxidil or propecia due to concerns about side effects.

The lava seawater used in the present invention is a unique water resource possessed only by Jeju Island as seawater is naturally filtered by basalt and sanisil layers and flows into the land, and has been aged under volcanic landform of Jeju Island for a long time. Due to its characteristics such as sex and cleanliness, lava seawater has been actively used as a breeding water for flounder farms. Since 5-6 years ago, it has been used as a spring water in the aspect of beauty, and it is receiving great attention. However, since 1980, researches on the utilization of such lava seawater have been conducted in terms of prevention of seawater infiltration in order to improve the restriction on the use of agricultural water and drinking water due to the high salt concentration of lava seawater. There were no studies on special applications other than breeding and sauna water.

Accordingly, the present inventors have made intensive research to overcome the problems of the prior art, and when using lava seawater as a hair growth agent composition, it is confirmed that the effect of promoting hair growth, preventing hair loss, or improving hair, and completed the present invention. Was done.

Therefore, the main object of the present invention is to provide a hair growth agent composition containing demineralized mineral water or concentrated mineral water having hair growth promotion, hair loss prevention, or hair improvement.

Another object of the present invention is to provide a hair restorer composition containing the demineralized mineral water or concentrated mineral water as an additive of an existing hair growth agent, and using the lava seawater demineralized mineral water as an extraction solvent to promote hair growth or hair loss prevention and improvement. It is to provide a composition that can be used to promote hair growth, prevent hair loss and improve hair by extracting natural products.

According to an aspect of the present invention, the present invention provides a composition for promoting hair growth or preventing hair loss and improving the lava seawater mineral water as an active ingredient. The lava seawater (or saltwater in the lava region) of the present invention is water that has been aged under the lava (volcanic) terrain for a long time by flowing the natural seawater in the lava region surrounded by seawater and then flowing into the basalt and sanilast layers. Lava seawater contains not only essential minerals such as sodium, magnesium, calcium and potassium, but also general useful minerals (iron, manganese, zinc, molybdenum, etc.) than deep seawater and deep water than general seawater and deep water. In addition, lava seawater contains a large amount of useful mineral components, among them, to stabilize the secretion of insulin, vanadium, which has the effect of treating diabetes, improving hyperlipidemia, promoting blood circulation, boosting immunity, and a large amount of germanium having anticancer activity. It contains. In addition, lava seawater is a clean groundwater resource in which no harmful components such as arsenic, mercury, cadmium, and the like are detected without E. coli, nitrate, phosphate, and phenols.

In the composition for promoting hair growth or preventing and improving hair loss of the present invention, the lava seawater mineral water may be used as drinking water when only the desalting step is used, and when the dehydration step is used, the salt composition is mixed with the mineral composition powder. In the case of both desalting and dehydration, it can be used as a pure (salt-free) mineral composition powder. In a preferred aspect, the lava seawater mineral water of the present invention is specifically Na, Mg, Ca, K, Cu, Mo, V, Ge, Sr, Si, Zn, Fe, Mn, Cl, B, Li, Ba, Pb And As. All of these minerals are minerals which have been found to be useful to the human body, and particularly include 1 to 100 ppb (μg / L) vanadium and 1 to 100 ppb (μg / L) germanium.

In the composition for promoting hair growth or preventing and improving hair loss of the present invention, the lava seawater mineral water may be obtained by desalting lava seawater taken from 100 to 200 m underground in Jeju Island, but preferably lava seawater taken from the eastern part of Jeju Island. It is good to use. In Example 1 of the present invention, 150 m underground was excavated from Handong-ri, Gujwa-eup, Bukjeju-gun, Jeju Island, and desalted Jeju lava seawater taken from 128 m underground (average sea level) to produce lava seawater demineralized mineral water. Mineral water was produced.

In the composition for promoting hair growth or preventing and improving hair loss of the present invention, the lava seawater demineralized mineral water is sterilized using a sterile filter, and then any method known in the art, for example, flash evaporation, seawater freezing, and reverse osmosis. Can be desalted by using ion exchange resin, electrodialysis or commercial dialysis or desalination, but desalination by electrodialysis can control mineral concentration in lava seawater demineralized mineral water by varying the conductivity. It is preferable because it is.

In the composition for promoting hair growth or preventing and improving hair loss of the present invention, the lava seawater concentrated mineral water (RO concentrated water) is sterilized using a sterile filter, and then any method known in the art, for example, flash evaporation or seawater. It can be concentrated using a freezing method, reverse osmosis method, ion exchange resin method, electrodialysis method or commercially available electrodialysis machine or demineralizer, but it is preferable to concentrate by reverse osmosis method.

In the composition for promoting hair growth or preventing and improving hair loss of the present invention, the hair growth promoting or hair loss prevention and improvement is a day for alleviating, preventing, or improving any hair loss symptoms that occur through a biological metabolic process activated by a signal factor that stimulates hair loss. Although, preferably, it is preferable to alleviate, prevent, or improve alopecia caused by promoting secretion of androgen, a male hormone, and more preferably, alopecia performed by inhibiting the activity of 5α-reductase. It is good to alleviate, prevent, or ameliorate the wound. This is caused by androgen (androgen), a masculine alopecia, and most of the hair loss patients undergo progressive gradual reduction of dermal papilla and hair follicles by dihydrotestosterone converted by Type II 5-alpha-reductase. Symptoms are promoted (Whiting D., J Am Acad Dermatol. 28, pp755-763, 1993; Singlair R., Br Med J. 317, pp865-886, 1998). In Example 3 of the present invention, it was confirmed that lava seawater demineralized mineral water inhibited the activity of 5α-reductase.

The hair growth promoting or hair loss prevention and improvement composition is hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisturizer cream, hair massager Cream, hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservative, hair dye, hair wave, hair bleach, hair gel, hair glaze, hair dresser, It may be prepared in the form of a hair lacquer, a hair moisturizer, a hair mousse or a hairspray. In addition, the composition may be used as a skin external preparation of a cream, gel, patch, spray, ointment, warning, lotion, linen, pasta or cataplasma formulation.

In the composition for promoting hair growth or preventing and improving hair loss of the present invention, the lava seawater demineralized mineral water has its own hair growth and hair loss prevention effect, so that it can be used directly as a hair growth agent composition. When added to other hair growth preparations containing natural extracts or compounds known to be effective, synergism may be caused to alleviate, prevent or improve hair loss symptoms, and promote hair growth. desirable. Examples of natural extracts that are effective in promoting hair growth and preventing hair loss include Munjuran, water lily, island gopipi, Jeju jeokdae, pine trees, non-tree, thyme, Halla gujeolcho, platinum, radish or radish, Halla leek, Jeju willow , Cornel, gingko tree, rock play, narcissus, hydrangea, walnut tree, hydrangea, tamra hydrangea, citrus fruit, lettuce, skin, ginger, black bean, black sesame, kelp, seaweed, mulberry leaf, pine needles, wolfberry, sancho, tocopherol , Seaweed, mulberry, medicinal herbs, wormwood, leaf, cheongung, snakeberry, thistle, migraine, jinjin wormwood, stag green, maple leaf and the like (hair growth composition and preparation method thereof, Registration No. 10-0519009-0000; as topical coatings and pills Method for producing a hair regrowth, registration number 10-0476731-0000; method for preparing a hair cleanser composition using the herbal extracts, and a composition prepared, registration number 10-0764874-0000; hair health maintenance and hair growth promoting raw material extract, registration number 10 -0747651- 0000), and examples of compounds that are effective for promoting hair growth and preventing hair loss are minoxidil, finasteride, glycyl-L-histidyl-L-lysine: copper (II) derivative (registered patent). 0111881-0000, 1997.02.10), 11,28-dioxa-4-azatricyclo (22.3.1.0.49) octacos-18-ene derivative (registered patent 10-0133916-0000), estrogen derivatives and salts thereof And hair growth promoting compositions containing the same (Application No. 10-1998-0019525), pyrimidine derivatives and retinoid combinations (Application No. 10-1988-0010083), hair growth as separate components for inducing and promoting hair growth and preventing hair loss. Non-immune inhibitory cyclosporine A derivative (application number 10-2000-0014837), hair growth promoter which has cyclosporin A 7-thioamide derivative as an active ingredient (application number 10-2000-0069394), and tolyrin External preparation composition (Application No. 10-2001-0064002) Monoterpenes hair cosmetic composition of the Amore Pacific whose primary component is a glucoside compound (Application No. 10-1993-0013746) and the like.

According to an aspect of the present invention, the present invention provides a composition for promoting hair growth or preventing hair loss and improving hair loss containing natural plant extracts using lava seawater demineralized mineral water as an extractant. The natural plant extract of the present invention may be any natural plant that is harmless to the human body, but is preferably a natural plant known in the art that has the effect of promoting hair growth or preventing hair loss and improving hair, such as Moonjuran, water lily, and island. Ogalpi, jeju jeoldae, solbi tree, non-tree, thyme, Halla gujeolcho, platinum, sunbi tree, halla leek, jeju mountain willow, wild berry, gingko tree, daedaeuk, narcissus, haekkuk, sakura tree, hydrangea, tamra Acorns, citrus fruits, cilantro, ginger, black bean, black sesame, kelp, seaweed, mulberry leaf, pine needles, wolfberry, sancho, tocopherol, seaweed, mulberry, medicinal herbs, wormwood, leafy leaves, celery, snakeberry, thistle, migraine Good for deer, deer rust, and maple leaves.

In the composition for promoting hair growth or preventing and improving hair loss of the present invention, the natural plant extract can be extracted using lava seawater demineralized mineral water alone, which has the effect of promoting hair growth or preventing hair loss and hair improvement, but is known in the art. It can be extracted by mixing with a variety of extraction solvents, preferably (a) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, etc.), (b) acetone, (c) ethyl acetate and (d) chloroform or (e) 1,3-butylene glycol, and may be mixed with an extraction solvent, and more preferably, it may be extracted and mixed with a lower alcohol having 1 to 4 carbon atoms. In Examples 6 to 11 of the present invention, extracts of natural plants were prepared using an extraction solvent obtained by mixing ethanol with lava seawater demineralized mineral water in various ratios. Extraction method of the natural extract in the present invention can be extracted using any extraction method known in the art, for example, hot water extraction, reflux cooling extraction, stirring extraction to stir with a stirrer, immersed for 1 to 15 days It is possible to extract using extraction methods such as immersion extraction to extract, and ultrasonic extraction using an ultrasonic generator. In addition, if necessary, in order to obtain a composition for promoting hair growth prevention or hair loss of the present invention, after the extraction solvent treatment may be subjected to an additional purification process. Hair growth through various additional purification methods, such as, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). A composition for promoting or preventing hair loss and improving can be obtained.

Hereinafter, the lava seawater demineralized mineral water of the present invention will be described in more detail step by step.

After excavating 150 m underground from Handong-ri, Gujwa-eup, Bukjeju-gun, Jeju-dong, Jeju Island, sterilized Jeju lava seawater collected at 128 m underground (based on average sea level) using a sterile filter. Desalination treatment by ion exchange resin method, electrodialysis method or desalting method selected during commercially available electrodialysis or desalting machine, and control the concentration of minerals in lava seawater demineralized mineral water by varying the conductivity. The demineralized mineral water and the mineral components of the lava seawater were obtained from the Korea Chemical Testing Institute and analyzed by ion chromatography (DX500, DIONEX, USA) and ICP / MS (X series, Thermo elemental, UK). The results are shown in Table 1.

TABLE 1 Comparative Analysis of Mineral Components in Raw Water, Desalted Mineral Water, and Concentrated Mineral Water

In addition, the specific manufacturing process of the natural extract extracted using the lava seawater mineral water of the present invention is as follows. Munjuran, water lily, citrus fruits, Seomok galpi, thyme, Halla gujeolcho, platinum, turnip tree, Halla leek, Jeju willow, Jeju jeokdae, pine trees, cornel, berry, birch, daedae, narcissus, Plants such as seaweed, horseradish tree, hydrangea, and tamra hydrangea are dried in a well-ventilated shade, and then about 1 to 20 times the weight of lava seawater mineral water alone or 1 to 2 carbon atoms. 4 lower alcohol and lava seawater mineral water of about 1: 0.1 (alcohol: lava seawater demineralized mineral water) to 1: 10, preferably 1: 0.2 to 1: 3, more preferably 1: 0.3 to 1: 1 A mixed solvent having a mixing ratio of hot water extraction, reflux cooling extraction, stirring extraction with stirring with a stirrer for about 1 hour to 5 days, preferably 2 to 24 hours at an extraction temperature of 20 to 100 ° C., and deposition for 1 to 15 days Sediment weight to extract It may be once to five times to extract such as ultrasonic extraction method using the ultrasonic generator, and preferably repeated twice or three times to obtain the natural sea water lava mineral extracts and natural mixed solvent extract.

The lava seawater mineral water obtained by the above manufacturing process is characterized by the hair growth effect in the hair follicle, dermal papilla cell proliferation effect and 5 alpha-reductase (5α-reductase) using rat vibrissa hair follicle isolation culture. As a result of checking the inhibitory effect, it has been confirmed that the hair growth promoting effect and the prevention of hair loss can be used as a hair growth composition.

Since the lava seawater mineral water of the present invention has its own hair growth and hair loss prevention effect, it can be directly used as a hair growth preparation, and added to other hair growth compositions containing natural extracts effective for promoting hair growth and prevention of hair loss. As it is also available in the form used to extract natural extracts, it is expected that it will be able to enhance the hair growth promotion and prevention of hair loss of existing hair growth preparations.

In addition, the hair growth agent composition containing the lava seawater mineral water of the present invention as an active ingredient may be used in cosmetics such as shampoos, rinses, hair tonics, hair care products, and external preparations that are applied directly or sprayed onto the scalp. The lava seawater mineral water is contained in an amount of 0.01 to 10% by weight, preferably 0.05 to 5% by weight, and more preferably 0.1 to 2% by weight, based on the total weight of the composition.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example 1. lava seawater desalination mineral water production

Jeju lava seawater excavated 150m underground from Handong-ri, Gujwa-eup, Bukjeju-gun, Jeju-do, Korea, and collected from 130m underground (average sea level) by using a microfiltration system of less than 5㎛ in microfiltration system to remove floating matters and foreign matters. Ultra Filtration A hollow fiber membrane is used to filter low molecular weight materials, polymers and colloidal materials with a pore filter ranging from tens to hundreds of nanometers (nm).

The lava seawater in the filtered aqueous solution state was subjected to direct ion exchange by applying direct current between the electrodes with an ion exchange membrane using an ED (Electrodialysis) device manufactured by ASTOM Corporation of Japan, causing selective ion exchange. Ion and dianion ions are removed. If the electrical conductivity is adjusted to 15, 12, 10, 8m / S, etc., the removal rate of monovalent and divalent ions will vary depending on the conductivity. The lava seawater demineralized mineral water desalted by using the ED device is passed through a sterilization process by passing through a UV / water sterilizer (SCUV-250, Sungchang EMC) that emits strong ultraviolet rays with a wavelength of 253.7 nm. Lose.

In addition, the filtered lava seawater was concentrated for 2 hours at 60 ℃ using a rotary evaporator (Rotavapor R-200, Buchi, Germany) to prepare a lava seawater concentrated mineral water.

Example  2. Rat vibrissa hair follicle By separating culture of Hair growth effect  Confirm

Males of Wistar rats (Samtako, Korea), 3 weeks old, were anesthetized with ethyl ether and cervical spine was killed. Rat left and right mystacial pads were isolated to isolate E / P buffer solution containing 100units / ml penicillin-100µg / ml streptomycin (Gibco Inc, NY, USA) [(50% Earle ′s balanced salts). solution; EBSS, Sigma MO, USA) + (50% Phosphate-buffered saline; PBS, Sigma MO, USA). The vibrissa follicles were carefully separated under an anatomical microscope. Separated hair follicles in a Petri dish containing E / P buffer solution, and incubated for a while in 37 ℃, 5% CO ₂ thermostat. 2 mM L-glutamine (Gibco Inc, NY, USA), 10 μg / ml insulin (Sigma MO, USA), 50 nM hydrocortisone (Sigma MO, USA) and 100 units / ml penicillin-streptomycin per well of a 24 well plate 500 μl of William E medium (Gibco Inc, NY, USA) was added, and one hair follicle was added per well and cultured at 37 ° C. in a 5% CO ₂ incubator while changing medium every three days. The morphology of vibrissa follicles in culture was observed under a microscope (Olympus, Japan). The positive control group was treated with minoxidil sulfate (minoxidil sulfate) at a concentration of 1 μM, and the lava seawater demineralized mineral water and the concentrated mineral water obtained in Example 1 were treated according to the concentration, and the hair follicle length was changed at 0 and 21 days. The growth was measured by comparing with the average length of the control group and the results are shown in Figure 1, 2, 3, 4. As a result, it could be observed that the demineralized mineral water or the concentrated mineral water of the present invention showed improved hair growth effect compared to the control group.

Example 3. Confirmation of 5 alpha-reductase inhibitory effect

Male Wistar rats 7-8 weeks old were inhaled with ethyl ether, slaughtered with cervical spine, and the prostate was removed and quenched with liquid nitrogen. Prostate weight was measured and 5 ml / g of 20 mM photassium phosphate buffer at pH 6.5 containing 0.32 mM sucrose (Sucrose; Amresco Inc, USA) and 1 mM Dithiothreitol (Sigma, USA) was added. The prostate was ground with a homogenizer and filtered by centrifugation at 1000 rpm for 10 minutes. The supernatant was recovered, centrifuged with an ultracentrifuge (Beckman, USA), and the precipitate was recovered and dissolved by adding 20 mM photassium phosphate buffer, which was used as a 5 alpha-reductase fraction. Protein concentration was standardized by BSA (Bovine serum albumin) and quantified using a Bio-Rad protein assay kit (Bio-Rad, USA). 250 μg of the 5 alpha-reductase fraction and 120 nCi [1,2,6,7-3H] -testosterone, 200 μM NADPH (Calbiochem, US), lava seawater demineralized mineral water and concentrated minerals in an Eppendorf tube. 500 μl of water was added to 40 mM potassium phosphate buffer at pH 6.6. After reacting at 37 ° C. for 30 minutes, 1 ml of ethyl acetate was added and centrifuged at 1000 rpm for 10 minutes. After recovering only the supernatant and drying under reduced pressure, 50 µl of ethyl acetate was added to dissolve the solution, which was then added to a TLC (Thin layer chromatography, Merck, Germany) plate. 50:50 (ethyl acetate: cyclohexane) was used as a developing solvent. Testosterone (Testosterone) was confirmed at 254nm using a UV lamp (UVGL-58, Upland, USA), dihydrotestosterone (dihydrotestosterone (DHT)) was confirmed by heating after sprinkling 10% sulfuric acid. The testosterone and dihydrotestosterone sites were cut out of the TLC plate, placed in a scintilation vial, and 5 ml of cocktail was added. The cpm (counter per minute) was measured using a liquid scintilation counter (Packard Instruments, USA). The conversion rate of testosterone by 5 alpha-reductase activity was calculated as the ratio of DHT / (testosterone + DHT) and expressed as% of the control group (FIG. 5). As a result, 5 alpha-reductase activity decreased by 23% compared to the control group when treated with lava seawater demineralized mineral water (12 ms).

Example  4. In dermal papilla cells  Proliferation effect and cytotoxicity

DMEM containing rat vibrissa immortalized dermal papilla cells containing 100 units / ml penicillin-100 μg / ml streptomycin (Gibco Inc, NY, USA) and 10% heat-inactivated fetal bovin serum (FBS; Gibco Inc, NY, USA) Hyclone Inc, USA) medium was used to culture in a 37 ° C, 5% CO ₂ incubator, and subcultured once every three days. Proliferation of rat vibrissa immortalized dermal papilla cells was measured by MTT assay. Dermal papilla cells (1.0 × 10 ⁴ cells / mL) were placed in 96 well plates and incubated for 24 hours, exchanged with serum-free DMEM medium, and cultured again for 24 hours, followed by lava seawater desalted mineral water and concentrated mineral water. . A positive control, minoxidil sulfate (Sigma, USA) was treated at a concentration of 1 μM. After incubation for 4 days, 50 μl of MTT (Sigma, MO, USA) was added and reacted for 4 hours. The supernatant was removed, 200 μl of DMSO was added to dissolve the precipitate, and the absorbance was measured at 540 nm using a microplate reader (Amersham Pharmacia Biotech, NY, USA). The average absorbance values for each sample group were obtained, and the degree of proliferation and toxicity were confirmed by comparing with the absorbance values of the control group. As a result, there was no significant proliferation effect and cytotoxicity in both lava seawater ED demineralized water and concentrated mineral water (Table 2).

TABLE 2 Proliferative effect and cytotoxicity on dermal papilla cells

† Sterilized is sterilized using autoclave

‡ Filtered is sterilized using sterilized filter.

Example  5. C57BL / 6 mouse Hair growth  Confirm

5-1. Experimental animal

Six-week-old female C57BL / 6 mice (Central Experimental Animal Co., Ltd.) were used in the experiment after adapting to the animal breeding environment for 1 week. Animal kennels were maintained day and night for 12 hours at a temperature of 23 ± 3 ° C. Experimental animals were reared in mouse cages for isolation and were fed freely with experimental feed. The experimental group was composed of 5 groups, which was a control group, a positive control group (5% minoxidil (modern drug), which is a drug generally used as a hair regrowth agent, and 10ms, 12ms, and 15ms of demineralized mineral water). Each 10 mice were used for the experiment.

5-2. Sample application and observation of hair growth efficacy

To examine the wool effect, we used 7-week-old resting hairs with pink color on the dorsal skin. The back of the mouse was cut with a small animal clipper, and applied to the dorsal skin of each group using a depilatory agent (Bikiro Cream, Taegeuk Pharm.) To completely remove the hair, and then the remaining hair removal agent was washed with water. ED demineralized mineral water and 5% minoxidil, which were samples, were dropped on the epilating site by 200 ul and then applied by tapping. After that, it was applied once a day, and PBS was applied to the control group. On the 1st, 14th, and 21st day after the start of the experiment, light anesthesia was performed with ethyl ether to visually check the hair growth state and photographs were taken (Philp D, et al., 2004). The results are shown in FIG.

Example  6. Lava seawater Desalination Mineral water  Used Moon Ju Ran Extract  Produce

Dried in the shade, 1kg of finely prepared Munjuran was put in 10L of lava seawater demineralized mineral water, stirred for 5 days at room temperature, filtered through a 250 mesh filter cloth, left to mature at 4 ° C for 7 days, and filtered through Whatman filter paper to extract 9.7L. (Solid content 117.6g) was obtained. Table 3 below describes the extraction mixed solvents and the results.

[Table 3]

Example  7. Lava seawater Desalination Mineral water  Preparation of used water extract

1 kg of dried water lily roots, which were dried in the shade, were extracted in the same manner as in Example 6 using lava seawater demineralized mineral water, and an extract 9.8L (solid content 65.7 g) was obtained. Table 4 below describes the extraction mixed solvents and the results.

[Table 4]

Example  8. Lava seawater Desalination Mineral water  Used Island Ogalpi Extract  Produce

1 kg of fine island roots dried and dried in the shade were extracted in the same manner as in Example 6 using lava seawater demineralized mineral water to obtain an extract 9.7 L (solid content 176.7 g). In Table 5, the extraction mixed solvents and the results are described.

TABLE 5

Example  9. Lava seawater Desalination Mineral water  Preparation of Jeju Joridae Extract Used

1 kg of Jeju chopped leaves, dried and dried in the shade, were extracted in the same manner as in Example 6 using lava seawater demineralized mineral water to obtain an extract of 9.5 L (solid 133.2 g). Table 6 below describes the extraction mixed solvents and the results.

TABLE 6

Example  10. Lava seawater Desalination Mineral water  Used Pine Tree Extract  Produce

1 kg of dried pine cone branches dried in the shade were extracted in the same manner as in Example 6 using lava seawater demineralized mineral water to obtain an extract 9.8L (solid content of 93.5 g). Table 7 below describes the extraction mixed solvents and the results.

 TABLE 7

Example  11. Lava seawater Desalination Mineral water  Manufacture of used non-tree extract

1 kg of dried non-branch branches dried in the shade were extracted in the same manner as in Example 6 using lava seawater demineralized mineral water to obtain an extract 9.6 L (solid content: 72.9 g). Table 8 below describes the extraction mixed solvents and the results.

[Table 8]

Hereinafter, the preparation examples of hair growth preparations for promoting hair growth prevention and hair loss containing the composition of the present invention will be described.

Formulation Example 1 Preparation of Hair Tonic

Formulation example  2. Of hair conditioner  Produce

Formulation example  3. Hair lotion  Produce

Formulation example  4. Hair soap  Produce

According to the present invention, lava seawater mineral water having hair growth promotion, hair loss prevention, or hair improvement may be usefully used as a hair growth agent composition or as an additive of an existing hair growth agent.

By using the lava seawater mineral water having the hair growth promotion, hair loss prevention, or hair improvement as an extraction solvent, it can be used to promote hair growth, hair loss prevention and hair improvement by extracting natural products that have been found to promote hair growth or hair loss prevention and improvement.

1 is a diagram showing the hair growth effect in the hair follicles of lava seawater RO concentrate confirmed using rat vibrissa hair follicle isolation culture.

Figure 2 is a diagram showing the hair growth effect of lava seawater demineralized mineral water (10m / S) hair follicle confirmed using the rat vibrissa hair follicle separation culture.

Figure 3 is a diagram showing the hair growth effect of lava seawater demineralized mineral water (12m / S) hair follicle confirmed using the rat vibrissa hair follicle separation culture.

Figure 4 is a diagram showing the hair growth effect of lava seawater demineralized mineral water (15m / S) hair follicle confirmed using the rat vibrissa hair follicle separation culture.

5 is a diagram showing the 5 alpha-reductase inhibitory effect of lava seawater demineralized mineral water and RO concentrated water.

6 is a diagram showing the hair growth effect using lava seawater demineralized mineral water and C57BL / 6 mice of the control group.

Claims (11)

A cosmetic composition for improving hair loss comprising lava seawater mineral water obtained by desalting lava seawater taken from 100 to 200 m underground in Jeju Island as an active ingredient. The cosmetic composition for improving hair loss of claim 1, wherein the lava seawater mineral water comprises 1 to 100 ppb of vanadium, and 1 to 100 ppb of germanium. delete The cosmetic composition for improving hair loss according to claim 1, wherein the lava seawater mineral water is desalted mineral water desalted by electrodialysis. The cosmetic composition for improving hair loss according to claim 1, wherein the lava seawater mineral water is concentrated mineral water concentrated by a reverse osmosis method. The cosmetic composition for improving hair loss according to claim 1, wherein the hair loss improvement is performed by inhibiting the activity of 5α-reductase. The cosmetic composition for improving hair loss according to claim 1, wherein the composition for improving hair loss further comprises the same ingredients as the active ingredients of the existing hair restorer composition. A cosmetic composition for improving hair loss, comprising a plant extract selected from the group consisting of munjuran, water lily, seol galpipi, jeju jeoldae, pine needles, and non-trees, using lava seawater mineral water as an extraction solvent. The cosmetic composition for improving hair loss according to claim 8, wherein the extraction solvent is a mixed extraction solvent of lava seawater mineral water and a lower alcohol having 1 to 4 carbon atoms. delete 10. The composition according to any one of claims 1, 2 and 4 to 9, wherein the composition is a hair tonic, a hair conditioner, a hair essence, a hair lotion, a hair nutrition lotion, a hair shampoo, a hair rinse, a hair treatment. , Hair cream, nourishment cream, hair moisturizer cream, hair massage cream, hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair drying agent, hair preservative, hair dye, hair wave , Hair bleach, hair gel, hair glaze, hair dresser, hair lacquer, hair moisturizer, hair mousse, hair spray, cream, gel, patch, spray, ointment, warning, lotion, lining, pasta and A cosmetic composition for improving hair loss, characterized in that the formulation is selected from the group consisting of cataplasma.

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