KR20170118359A - Cosmetic composition containing gynostemma pentaphyllum leaf extract - Google Patents

Abstract

The present invention relates to a cosmetic composition containing an extract of a stone-on-the-leaf, and more particularly to a cosmetic composition containing an extract of water and a stone-to-the-leaf, wherein the extract of the stone-to-leaf comprises 0.1 to 10% , An antioxidant, a whitening and a wrinkle-improving effect, and can inhibit the growth of bacteria and increase the preservation period.

Description

[0001] Cosmetic composition containing gynostemma pentaphyllum leaf extract [0002]

The present invention relates to a cosmetic composition containing an extract of a stone-on-the-leaf, and more particularly to a cosmetic composition containing an extract of a stone and a leaf, The present invention relates to a cosmetic composition containing an extract of a stone-on-the-leaf extract capable of increasing the period of time.

Skin is an epithelial tissue that covers and protects the muscles and organs of our body. It consists of epidermis, dermis, subcutaneous fat, protects the body from external environment and regulates body temperature. When the pollutants such as ultraviolet rays, tobacco smoke, and soot are added to the skin or the aging proceeds, active oxygen is produced. When the active oxygen is not effectively removed by various protective devices in the body, a series of inflammation reaction occurs, Damage is caused. Inflammation reaction reduces the water content of the stratum corneum and makes it dry, causing the elastic fibers of the dermal layer to denature, promoting skin aging. Accordingly, various cosmetic compositions have been developed as described in the following patent documents in order to minimize skin damage.

<Patent Literature>

Patent Publication No. 10-1216410 (Registered Dec. 21, 2012) "Cosmetic Composition for Functionally Essential Skin with UV Protection Containing Extract of Stones and Root Extract"

However, the conventional cosmetic composition containing the off-shore leaves has a limited effect such as skin soothing, requires a large number of materials because of low extraction efficiency of the active ingredient, and is easily proliferated by bacteria, .

SUMMARY OF THE INVENTION The present invention has been made to solve the above problems,

It is an object of the present invention to provide a cosmetic composition containing an extract of an extract from a stone having improved skin whitening, wrinkle improvement, antioxidation and skin soothing effect.

It is another object of the present invention to provide a cosmetic composition containing an extract of a stone-on-the-fly extract which contains hydrogen water to inhibit the growth of bacteria and increase the preservation period.

The present invention also relates to a method for extracting water-soluble useful components from alcohols by first extracting the oil-soluble useful components by alcohol extraction, And to provide a cosmetic composition containing the same.

In order to achieve the above object, the present invention is implemented by the following embodiments.

According to one embodiment of the present invention, the cosmetic composition containing the extract from the outer part of the stones according to the present invention is characterized in that it contains hydrogen peroxide and extract from the outer part of the stones.

According to another embodiment of the present invention, in the cosmetic composition containing the extract from the outer part of the stones of the present invention, the hydrogenated water is formed by injecting hydrogen gas into water and dissolving it.

According to another embodiment of the present invention, in the cosmetic composition containing the extract of the plant extract from the stones according to the present invention, the hydrogen concentration is characterized by a dissolved gas concentration of hydrogen gas of 100 to 1500 ppb.

According to still another embodiment of the present invention, in the cosmetic composition containing the extract from the outer part of the stalk of the present invention, the extract of the outer part of the stalk is characterized in that hydrogen is used as an extraction solvent.

According to still another embodiment of the present invention, in the cosmetic composition containing the extract from the outer layer of a stone according to the present invention, the extract of the outer layer of the stone uses an alcohol as an extraction solvent to form an extract from the outer layer of the stone, And a water extract of the extraneous leaf water is formed by using water of a certain temperature as the extraction solvent with respect to the remaining amount of the stone outer leaves, and the extraneous water of the leaf water and the extruded alcohole extract are mixed.

According to still another embodiment of the present invention, in the cosmetic composition containing the extract from the outer part of the stones of the present invention, the cosmetic composition is characterized by having skin whitening, wrinkle reduction, antioxidation and skin soothing effect.

According to the present invention, the following effects can be obtained by this embodiment.

The present invention has an effect of improving skin whitening, wrinkle improvement, antioxidation and skin soothing effect.

Further, the present invention has the effect of inhibiting the growth of bacteria and increasing the storage period by including hydrogen water.

The present invention also relates to a method for extracting water-soluble useful components from alcohols by first extracting the oil-soluble useful components by alcohol extraction, There is an effect.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, a cosmetic composition containing an extract of a stone-on-the-fly according to the present invention will be described in detail with reference to the accompanying drawings. Unless defined otherwise, all terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs and, if conflict with the meaning of the terms used herein, It follows the definition used in the specification. Further, the detailed description of known functions and configurations that may unnecessarily obscure the subject matter of the present invention will be omitted. Throughout the specification, when an element is referred to as "including " an element, it is understood that the element may include other elements as well, without departing from the other elements unless specifically stated otherwise.

The cosmetic composition according to one embodiment of the present invention includes an extract of a stone-leaf extract and hydrogenated water. Gynostemma pentaphyllum is a perennial vine plant with dicotyledonous plant leaves. Leaves grow in mountains or forests. Roots grow sideways, with white hairs on the nodes, tangled and growing, It is a compound leaf with leaves and it has a narrow egg-shaped oval or narrow oval shape. The hydrogenated water refers to water containing hydrogen gas in water. Hydrogen may be contained in water through electrolysis. However, in order to minimize the content of impurities, hydrogen is directly injected into water and dissolved. It is preferable that the hydrogen concentration is such that the dissolved concentration of the hydrogen gas (H ₂ ) is at least 100 ppb to at most 1500 ppb.

It is preferable that the above-mentioned out-of-stone-leaf extract is formed of an out-of-plant water of leaves or an out-of-stone alcohol extract, and an alcohol such as ethanol is used as an extraction solvent to form an out- (70 to 90 ° C) is used as an extraction solvent to form a water extract of extraneous leaf water, and it is more preferable that the extract is formed by mixing the water extract of the extreme off-the-leaf water and the extract of the extracellular alcohols.

The extracellular step extract is subjected to alcohol extraction and then subjected to extraction with hydrogen peroxide at a temperature of 70 to 90 ° C. This is because if heat is first applied to the extraction of hydrogen peroxide, And water extraction is performed first, there arises a problem that the percentage of water contained in the stone outer leaves varies. To prevent this, it is preferable to perform alcohol extraction and then water extraction.

The above-mentioned extracellular alcohols extract and extragranular alfalfa extracts can be prepared as follows. The outer side of the stone is cleaned with water, dried in the shade or sunlight, and pulverized to an appropriate size, and the crushed stone outer leaf is treated with a polar solvent selected from a 10-fold weight of a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, Ethanol is preferably used, more preferably 70% alcohol is used for leaching for 10 to 30 days, and then filtered to produce an extract of alveoli. It is understood that such an extract of alcohol such as lipids, which are not soluble, is highly extracted. In addition, after obtaining a stone-leaf alcohol extract, hydrolyzed water is added to the remaining amount of the stones, the mixture is stirred at 70 to 90 ° C for 2 to 6 hours, and then filtered to produce an extraneous leaf water extract. The extraction of water - soluble polysaccharides and other substances are highly appreciated by the extraction of water, and it is understood that the extraction efficiency of the useful components is increased when using the hydrophobic water.

According to the present invention, the cosmetic composition according to the present invention contains an extract of hydrolyzed water and an extract from a stone to improve skin whitening, wrinkle, antioxidant, skin soothing effect, inhibit bacterial growth by hydrogen peroxide, After the extraction, extraction with water is carried out to extract both the fat-soluble oil component and the water-soluble oil component, and extracting useful components by using water instead of purified water. These effects will be described in detail in the following examples.

The cosmetic composition of the present invention may further comprise a composition selected from the group consisting of water-soluble vitamins, oiliness vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

As the water-soluble vitamin, any of vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C and vitamin H can be used, And salts thereof (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbic acid-2-phosphate etc.) can also be used as water-soluble vitamins . The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

As the above-mentioned useful vitamins, any vitamins A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) , And derivatives thereof (such as palmitic acid ascorbin, stearic acid ascorbic acid, dipalmitic acid ascorbic acid, dl-alpha tocopheryl acetate, dl-alpha tocopherol nicotinic acid vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, Ethyl ether, etc.) and the like are also included in the usable vitamins used in the present invention. The usable vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method.

The polymeric peptide may be any compound as long as it can be compounded in a cosmetic, and preferably collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms .

The polymeric polysaccharide may be any compound as long as it can be compounded in cosmetics, and examples thereof include hydroxyethylcellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

The sphingogly lipid may be any as long as it can be compounded in cosmetics, and preferable examples thereof include ceramides, phytosphingosine and sphingoglycolipids. The sphingogly lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by a conventional method or can be obtained by chemical synthesis.

The seaweed extract may be any of those which can be compounded in cosmetics, but preferably include algae extract, red tiger extract, green algae extract and the like, and furthermore, coloring guanine, arginic acid, sodium alginate , Potassium alginate, and the like are also included in the seaweed extract used in the present invention. The seaweed extract can be obtained from seaweed by a conventional method.

The cosmetic composition of the present invention may contain other ingredients usually added to the cosmetic composition. For example, it is possible to use various additives such as a preservative, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a plant extract, a pH adjuster, Cold agents, antiperspirants, purified water, and the like.

Examples of the above-mentioned fat-soluble ingredients include ester-based fats, hydrocarbon-based fats, silicon-based fats, fluorine-based fats, animal fats and plant fats and the like. Examples of the ester-based oil include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearyl isostearate, But are not limited to, ethylbutyl, butylbutyl, ethylbutylbutyl, butylbutyl, isobutylbutyl, isobutylbutyl, isobutylisobutyl, isobutylisobutyl, isobutylisobutyl, Trimethylol propane, triisostearic acid trimethylol propane, tetra-2-ethylhexanoic acid pentaerythritol tri (2-ethylhexanoic acid) But are not limited to, tallow, caprylic estersyl, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, myristine sasyl, stearyl stearate, decyl oleate, Examples of the isocyanates include isophorone diisostearate, isophorone diisostearate, isophorone diisostearate, isophorone diisostearate, isophorone diisostearate, isophorone diisostearate, isophorone diisostearate, Ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol , Propylene glycol dicaprylylate, dicapric acid neopentyl glycol, dioctanoic acid neopentyl glycol, tricarboxylic acid glyceryl, triunsaturated acid glyceryl, triisopalmitic acid glyceryl, triisostearic acid glyceryl, neopentanoic acid octyl Dodecylsuccinic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, Polyglycerin isostearic acid ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, octyldecyl lactate, octyldecyl isostearate, polyglycerin oleic acid ester, But are not limited to, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, Propyl stearate, propyl stearate, propyl stearate, stearyl stearate, stearyl stearate, propyl stearate, stearyl stearate, Stearyl hydroxystearate, stearyl 12-stearoyl hydroxystearate, 12-stearoyl hydroxystearic acid, Allo one hydroxy stearic acid and the like esters such as cetearyl source. Examples of the hydrocarbonaceous oil include hydrocarbons such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline. Examples of the silicone-based oil include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethyl polysiloxane, decamethyl polysiloxane, dodecamethyl cyclosiloxane, dimethyl siloxane methyl cetyloxysiloxane copolymer, dimethyl siloxane methyl stearoxysiloxane copolymer, Alkyl-modified silicone oil, and amino-modified silicone oil. Examples of the fluorine-based oil include perfluoropolyether and the like. Examples of the animal or plant oil include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, The present invention relates to a process for producing a starch-containing starch, which is selected from the group consisting of seed oil, cottonseed oil, palm oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, Animal or vegetable fats such as oil, can-pick wax, carnauba wax, liquid lanolin, and hardened castor oil.

Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers. Examples of the water-soluble low molecular weight moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Propylene glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like. Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol, cholesterol ester and the like. Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid salt, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, . Examples of the oil-soluble polymer include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the molient agent include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants. Examples of the nonionic surfactant include self emulsifying monostearic acid glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene), sorbitan fatty acid ester, POE sorbit fatty acid ester POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POEPOP (polyoxyethylene polyoxypropylene) copolymer, POEPOP alkyl ether, polyether modified silicone, lauric acid alkanolamide , Alkylamine oxides, hydrogenated soybean phospholipids, and the like. Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylallylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, alkyl Amide phosphate, alkyloyl taurine salt, N-acyl amino acid salt, POE alkyl ether carboxylate, alkylsulfosuccinate, sodium alkylsulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester and the like . Examples of the cationic surfactant include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium bromide, Benzalkonium chloride, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like. Examples of the amphoteric surfactant include carboxybetaine, amidebetaine, sulfobetaine, hydroxysulfobetaine, amidosulfobetaine, phosphobetaine, aminocarboxylate, imidazoline derivative, amideamine, etc. , And the like.

Examples of the organic and inorganic pigments include silicic acid, anhydrous silicic acid, magnesium silicate, talc, sericite, mica, kaolin, spinach, clay, bentonite, titanium coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, Inorganic pigments such as aluminum, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium oxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.

Examples of the organic powder include metal soaps such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.

Examples of the ultraviolet absorber include ultraviolet absorbers such as p-aminobenzoic acid, ethyl p-aminobenzoate, amyl paranobenzoate, octyl p-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, Benzyl, 2-ethoxyethyl methoxycinnamate, octyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl methoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Dihydroxymethoxybenzophenone sulfonic acid, dihydroxybenzophenone sulfonic acid, dihydroxybenzophenone sulfonic acid and its salt, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzo Phenanone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino-p- (carbo-2'-ethylhexyl- 1,3,5-triazine, 2- (2- And the like can be de-hydroxy-5-methylphenyl) benzotriazole.

Examples of the bactericide include antibiotics such as hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, No. 301, mononitro and eicoll sodium, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, any of the above components may be blended within the range not impairing the object and effect of the present invention, but is preferably 0.01 to 5% by weight based on the total weight, More preferably 0.01 to 3% by weight.

The cosmetic composition of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients conventionally used in cosmetic compositions in addition to the above-mentioned compounds, for example, conventional additives such as stabilizers, solubilizers, vitamins, And a carrier.

The cosmetic composition of the present invention can be prepared in any formulations conventionally produced in the art, and examples thereof include emulsions, creams, foundations, lotions, essences, and hair cosmetic compositions. Specifically, the cosmetic composition of the present invention can be used as a skin lotion, a skin softener, a moisturizing lotion, a nutritional lotion, a massage cream, a nutrition cream, a hand cream, a moisturizing cream, an essence, a pack, a soap, a lotion, a milk lotion, Cleansing foams, body cleansers, astringents, and spray formulations. When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component . When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether. In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters. When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used. When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these are only for the purpose of illustrating the present invention in more detail, but the scope of the present invention is not limited thereto.

&Lt; Example 1 >

The 20 days immersion of the crushed stone oeip in 70% ethanol of 10 times the weight after the filtering by the dissolved stone oeip alcohol preparing the extract, filtered, and the number of 10 times the weight of the remaining stones oeip remaining minority (hydrogen gas (H ₂₎ And the mixture was heated at 80 ° C. for 4 hours with stirring. The resulting mixture was filtered to prepare an extraneous leaf water extract, which was then mixed at a ratio of 1: 1 The extract from the leaves was concentrated, concentrated under reduced pressure and lyophilized to prepare a sample.

&Lt; Example 2 >

The crushed stone outer leaves were immersed in 70% ethanol of 10 times weight for 20 days and then filtered to prepare an extract of the stone outer alveoli. To the remaining amount of the remaining stone outer leaves, purified water having a weight of 10 times the weight was added and stirred at 80 ° C for 4 hours After the addition of heat, the extract was filtered to prepare a purified water extract of stone outer, and then the extract of purified water from the outer side of the stone and the extract of the stone outer alcohols were mixed in a ratio of 1: 1 to produce an extract of the outer layer of the stone, concentrated under reduced pressure and lyophilized to prepare a sample .

&Lt; Comparative Example 1 &

The crushed stone outer leaves were dipped in 10 times by weight of 70% ethanol for 20 days and then filtered to produce a stone-leaf alcohol extract (stone-leaf extract), concentrated under reduced pressure, and lyophilized to prepare a sample.

&Lt; Comparative Example 2 &

The crushed stone outer leaves were placed in a 10-fold weight of hydrogen peroxide (the dissolved concentration of hydrogen gas (H ₂ ) was 1500 ppb), heated at 80 ° C for 4 hours while stirring, and filtered to remove the extraneous leaf water water- , Concentrated under reduced pressure, and lyophilized to prepare a sample.

&Lt; Comparative Example 3 &

Hydrogen water (hydrogen gas (H ₂ ) dissolved concentration of 1500 ppb) of 10 times the weight of the crushed stone outer leaves was added and heated at 80 ° C for 4 hours while stirring to prepare a purified water extract of the stone outer product, The remaining stone leaves were immersed in 70% ethanol of 10 times by weight for 20 days and then filtered to prepare a stone-leaf alcohol extract. The purified water extract of the stone-leaves and the stone-leaf alcohol extract were mixed in a ratio of 1: 1, An extract was prepared, concentrated under reduced pressure and lyophilized to prepare a sample.

<Experimental Example 1> Evaluation of antioxidative effect by DPPH radical scavenging activity

1) Dissolve 2.5 mg of DPPH in 17 mL of ethanol to prepare a DPPH solution. To 10 mL of this solution, 5.75 mL of DMSO was added, and UV-Vis of the control group was observed at 517 nm. It was diluted with ethanol to an absorbance of 0.94-0.97 and shaken for 20 seconds. To 1 mL of the solvent, 1 mg of the sample of each of the extracts of the out of stone extract prepared in Examples 1 and 2 and Comparative Examples 1 to 3 was mixed and dissolved sufficiently. Then, 50 μL of the sample solution was added to 450 μL of the prepared DPPH, and the mixture was kept at room temperature for 10 minutes. Absorbance was measured. The elimination ability formula is calculated in the following manner and is shown in Table 1. Electron donating ability (%) = 100 - [(O.D. Of sample / O.D. of control)] 100

2) As shown in Table 1, it can be seen that Examples 1 and 2 are much higher than those of Comparative Examples 1 to 3, and thus it can be seen that the antioxidative power is high when extracted with alcohol (purified water) after alcohol extraction. In addition, it can be seen that Example 1 has a higher value than Example 2, and it can be seen that the antioxidant power is higher when water is used as the extraction solvent than purified water.

Example 1 Example 2 Comparative Example 1 Comparative Example 2 Compare to 3 Electron donating ability (%) 37 32 23 24 25

<Experimental Example 2> Evaluation of skin whitening effect by melanin biosynthesis inhibiting ability

1) B16F10 cells cultured in DMEM medium were subcultured to a concentration of 2.0 × 10 ⁴ cells / mL on a 24-well plate, cultured for 24 hours at 5% CO ₂ and 37 ° C., and melanin was produced by adding 100 nM α- Each of the extracts of the out of stone extract (concentration 1000 μM) prepared in Examples 1 and 2 and Comparative Examples 1 to 3 was added and cultured for 3 days. After completion of the incubation, the medium was removed, washed with phosphate buffer solution (pH 7.4), 1N NaOH was added to melanin, reacted at 80 ° C for 1 hour and absorbance was measured at 405 nm in a spectrophotometer. Inhibition of biosynthesis was expressed by the percentage of absorbance reduction (%) of sample solution added and no added sample solution.

2) As shown in Table 2, it can be seen that the amounts of melanin in Examples 1 and 2 are much smaller than those of Comparative Examples 1 to 3, and thus the whitening effect is high when extracted with alcohol (purified water) after alcohol extraction . In addition, it can be seen that Example 1 is lower in value than Example 2, and it can be seen that the use of hydrogen peroxide rather than purified water as an extraction solvent can enhance the whitening effect.

No additives Example 1 Example 2 Comparative Example 1 Comparative Example 2 Compare to 3 Amount of melanin (%) 100 50 58 78 73 71

<Experimental Example 3> Evaluation of wrinkle improvement effect by collagen synthesis ability

1) Human fibroblast dermal cell HDFn (American Type Culture Colletion (ATCC-CRL-2076)) was cultured in DMEM / F12 (3: 1) medium containing 10% fetal bovine serum and 1% penicillin- streptomycin. Were cultured at 37 ° C under 5% CO ₂ .

2) The HDFn was inoculated into a 96-well plate at a concentration of 1 × 10 ⁵ cells / ml, maintained at 37 ° C. under 5% CO ₂ for 24 hours, and then cultured in DMEM / F12 (3: 1) After the replacement, the extracts of each of the stones (concentration 1000 μM) prepared in Examples 1 and 2 and Comparative Examples 1 to 3 were added and cultured for 48 hours. Subsequently, the supernatant was collected and the amount of procollagen was measured using a Procollagen type 1 C-peptide (PIP) EIA kit. The results are shown in Table 3.

2) As shown in Table 3, it can be seen that the amounts of collagen in Examples 1 and 2 are much higher than those of Comparative Examples 1 to 3, and therefore, the effect of improving wrinkles is high when extracted with alcohol (purified water) after alcohol extraction . In addition, it can be seen that Example 1 has a higher numerical value than that of Example 2, and it can be seen that the effect of improving wrinkles can be enhanced when using water less than purified water as an extraction solvent.

No additives Example 1 Example 2 Comparative Example 1 Comparative Example 2 Compare to 3 amount of type 1 procollagen (ng / ml) 2000 2700 2500 2200 2250 2300

<Example 4> Evaluation of skin irritation mitigation effect by skin patch test

1) The cosmetic compositions containing the samples of the extract of the out of stone extracts prepared in Examples 1 and 2 and Comparative Examples 1 and 2 were prepared in the composition ratios shown in Table 4 below.

Content (% by weight) ingredient Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 The sample of Example 1 One - - - - One The sample of Example 2 - One - - - - The sample of Comparative Example 1 - - One - - - The sample of Comparative Example 2 - - - One - - The sample of Comparative Example 3 - - - - One 0 Vitamin E 0.2 0.2 0.2 0.2 0.2 0.2 Liquid paraffin 5 5 5 5 5 5 maintain 6.0 6.0 6.0 6.0 6.0 6.0 Ultraviolet absorber 2.0 2.0 2.0 2.0 2.0 2.0 Camellia oil 3.0 3.0 3.0 3.0 3.0 3.0 Flowing isoparaffin 2.0 2.0 2.0 2.0 2.0 2.0 antiseptic 0.01 0.01 0.01 0.01 0.01 0.01 Spices 0.5 0.5 0.5 0.5 0.5 0.5 Water Balance Balance Balance Balance Balance - Purified water - - - - - Balance Sum 100 100 100 100 100 100

2) Twenty 20 - to - 30 - year - old women who have never been hypersensitive to skin irritation and currently have no skin disease or skin allergy symptoms were tested for skin irritation by human skin patch test. Samples 1 to 5 in which 5.0% of lactic acid, which is a skin irritant, was added as a stimulating agent were used. First, the test area was wiped with 70% ethanol, dried, and 15 μg of the prepared test substance was dripped into a fin chamber (Finx chamber, 100 × 10, EPITEST, Finland), and the test piece was hermetically sealed on the inside of the forearm . After 24 hours of exposure, the patches were removed and marked with the marking pen. After 1 hour and 24 hours after the labeling, the test site was observed using a magnifying glass (8MC-150, DAZOR) to observe erythema and edema. The skin response (mean response rate) was calculated in the following manner and is shown in Table 5. Skin reaction rate = score × reaction number × 100 × 1/2) / [3 (maximum score) × total number of subjects (n)] (score of skin reaction is 0 (no response), 0.5 (faint or mild erythema) , 1 (marked erythema but weak erythema), 2 (marked erythema, papules and smallpox), 3 (severe erythema and algebra))

3) As shown in Table 5, samples 1 and 2 showed higher skin irritation mitigating effect than samples 3 and 5, and skin irritation mitigation was excellent when extracted with alcohol (purified water) after alcohol extraction .

Response (%)
(n = 20) Sample 1 + 5% lactic acid 3.35 Sample 2 + 5% lactic acid 3.40 Sample 3 + 5% lactic acid 5.90 Sample 4 + 5% lactic acid 6.50 Sample 5 + 5% lactic acid 6.20

&Lt; Example 5 > Evaluation of the effect of increasing the storage period by an experiment for measuring the number of general bacteria

Samples 1 and 6 of Example 4 were allowed to stand at room temperature for 48 hours, and then general bacterial counts were calculated using an ATP measuring instrument (portable bacterial contamination measuring instrument). The bacterial counts (BLU) of Sample 1 were 300, 6 had a bacterial count of 1300. Thus, it can be seen that when hydrogen water is used in the cosmetic composition, the proliferation of bacteria can be suppressed and the preservation period of cosmetics can be increased.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, Should be interpreted as belonging to the scope.

Claims (6)

Wherein the extract is a mixture of water and an extract of stony leaves. The method of claim 1,
Characterized in that the composition is formed by injecting hydrogen gas into water and dissolving it. 3. The method of claim 2,
Wherein the hydrogen gas has a dissolved concentration of 100 to 1500 ppb. 2. The method according to claim 1,
A cosmetic composition comprising an extract of a stone-on-the-leaf extract characterized by using hydrogen as an extraction solvent. 2. The method according to claim 1,
Extracting the extruded leaf water with an alcohol extract as an extraction solvent, extracting the extract from the extruded leaf water using an alcohol as a solvent, And an extract of a stone-on-the-wall alcohols. The cosmetic composition according to claim 5, wherein the cosmetic composition comprises
A cosmetic composition comprising an extract of a stone-on-the-leaf extract, which has skin whitening, wrinkle improvement, antioxidation and skin soothing effect.