KR101216410B1 - Composition of sun screening functional cosmetics for skin soothing effect using gynostem extract and resverol extract - Google Patents

Abstract

The present invention relates to a sunscreen composition which contains a stone extract and a long root extract with a continuous skin soothing effect, and has an excellent antioxidant power in order to suppress the generation of active oxygen due to the ultrafine titanium dioxide containing physical sunscreen The present invention relates to a sunscreen functional cosmetic composition that contains a stone extra leaf extract and e.

Description

Composition of Sun Screening Functional Cosmetics for Skin Soothing Effect using Gynostem Extract and Resverol Extract}

The present invention relates to a sunscreen functional cosmetic composition, and in particular, to inhibit the generation of free radicals due to the ultrafine titanium dioxide containing physical sunscreen, containing an excellent stone extract extract excellent in antioxidant power, at the same time containing The present invention relates to a skin calming sunscreen functional cosmetic composition containing a monocotyledonous extract and a long root muscle extract which exhibit a sedating effect by solving the phototoxicity problem by providing a continuous antioxidant power by imparting an electron donating ability.

Ultraviolet rays form vitamin D in the skin to promote bone growth, inhibit rickets, and promote the differentiation of epidermal cells to strengthen the skin barrier. However, ultraviolet rays are known to cause skin blackening, erythema, wrinkles caused by collagen destruction, and even skin cancer.

A lot of researches have been conducted since sunscreens for inhibiting UV rays are classified as functional cosmetics. Sunscreens can be classified into physical and chemical blockers. Physical blocking agents scatter ultraviolet rays to prevent ultraviolet penetration into the skin, and chemical blocking agents absorb ultraviolet rays to inhibit ultraviolet penetration into the skin.

Physical blockers are excellent in blocking action and do not have side effects such as contact dermatitis, but because they are opaque, when combined with a lot of creams or lotions, there is an aesthetic disadvantage. On the other hand, UV absorbers, which are chemical blockers, are aesthetically pleasing because they are transparent after being applied to the skin. Recently, due to the problem of contact dermatitis caused by a chemical absorber, a UV absorber, mainly in Europe, it is trying to legally regulate the use of physical blockers.

Physical barriers are opaque materials that reflect or disperse ultraviolet rays and visible light, and titanium dioxide (TiO ₂ ) and zinc oxide (ZnO) are used. Titanium dioxide has a refractive index of 2.72 and zinc oxide has a refractive index of 2.02, which is favored by titanium dioxide. Titanium dioxide has excellent UV-blocking effect and small side effects of contact dermatitis, but it is not good for cosmetic because of opacity of white matter. Recently, research to reduce the particle size of titanium dioxide in order to reduce the clouding phenomenon is in progress. Ultrafine titanium dioxide with reduced particle size has been studied a lot because of less whitening and better usability. However, in the case of ultrafine titanium dioxide, when the particle size is reduced to 390 nm or less, the following mechanisms are used: active oxides such as superoxide radical, singlet oxygen, hydroxy radical, and hydroperoxy It generates a radical (hydroperoxy radical).

Although it is not toxic itself, it absorbs ultraviolet rays and is chemically activated to generate free radicals, which are toxic. Such substances are called phototoxic substances.

The problem is that when titanium dioxide, the most commonly used sunscreen, is processed into ultra-fine particles to solve the clouding phenomenon, it does not have its own toxicity, but when applied to the skin, it becomes a phototoxic substance that generates free radicals by ultraviolet rays. Is the point.

The present invention has been invented in order to solve the above problems, the present invention has a synergistic effect by containing together the stone extracts and ephedra root extracts in order to solve the problem of active oxygen generation and phototoxicity caused by ultra-fine titanium dioxide, The purpose of the present invention is to provide a soothing sunscreen functional cosmetic composition that can solve the skin soothing effect, sunscreen effect, and the problem of clouding with continuous antioxidant effect.

The skin soothing sunscreen functional cosmetic composition containing the extra stone leaf extract and the root extract of the present invention is used in a sunscreen functional cosmetic containing ultrafine titanium dioxide having a particle size of 370 nm or less,

It is characterized in that it comprises 0.1 to 30.0% by weight of the stone extract extracted from the leaves of the stone other than ethanol, and 0.1 to 30.0% by weight of the extract of the root of the root of the extract using ethanol.

The skin calming sunscreen functional cosmetic composition according to the present invention has a sedative effect on skin with a continuous antioxidant effect by containing a complex of foliar leaf extract and P. aeruginosa extract containing a large amount of saponin components due to the generation of active oxygen and phototoxicity caused by the use of ultrafine titanium dioxide And sunscreen effect, and cloudiness can be solved.

The skin calming sunscreen functional cosmetic composition according to the present invention is characterized in that it comprises a stone extract extract and splenic root extract.

Dol-eo is a perennial vine herb belonging to the family of fruits, distributed in southern Korea, southern China, Japan, etc., and native to southern islands and Jeju islands. The leaves are alternate, and the leaf is made up of five small leaves. Flowers bloom yellow-green in August-September. Fruit is berry, 6-8 cm in diameter, also called Kyogodam. In the present invention, an extract extracted from the leaves of the dodol is used, and the main components of the dodol leaf extract used in the present invention include dammarane-based saponins, gypenoside and other primerosides (promeveroside), It contains glycosides, such as sophoriside, bisdesmoside, gentiobioside and rutionoside, and steroids, sugars and pigments. Damaran-based saponins isolated from dodol leaves are the same as the saponins of ginseng, and six new zipenosides are known as glycosides of the same family. Its structure is also revealed. Zipenoside also has obesity, hypertension, anti-inflammatory, anti-diabetic, immune function, cholesterol-enhancing action, cardiovascular disease prevention, Jinhae, expectoration, tonic action.

Hojang-geun is dried dried roots and rhizomes of the Mardipulaceae including perennial herbaceous ginseng, king-ho and round-leafed soybean paste. Hojang-geun has been used as a diuretic, diuretic, and sedative in the private sector since ancient times. It has been used in traditional medicine in Asian and other countries to treat various diseases such as purulent dermatitis, urinary tract infection, cystitis, hypertension and cancer arteriosclerosis. However, no disclosure or teaching has been made of the skin soothing effect of sunscreen on extracts.

Thus, the present inventors have been found that the antioxidant effect of the foliar leaf extract shows a calming effect of the skin while researching on a sunscreen that can be safely used without side effects on the skin to eliminate the active oxygen caused by ultra-fine titanium dioxide It was confirmed that it could not be expressed, and due to the electron donating effect of the root extract, it is a network antioxidant system that allows the extracellular leaf extract to have a continuous antioxidant effect, which shows excellent skin soothing effect that does not cause synergistic phototoxicity. It was confirmed that the present invention was completed.

That is, in the present invention, the active oxygen generation and phototoxicity problems caused by the use of ultra-fine titanium dioxide are combined with a stony leaf extract and a long root extract containing a large amount of saponin. The problem could be solved. Among the saponin components contained in the monocotyledonous extract, it contains a large amount of phenenoside, which is a potent ingredient showing a major antioxidant effect. In addition, the extract of E. coli may have anti-inflammatory effect and electron donating effect by containing resveratrol. In particular, in the case of Jeju acid it is more effective because it contains a large amount of these components.

Let's look at this in more detail.

Free radicals produced by ultra-fine particulate dioxide have free radicals. Radicals take electrons from other molecules and oxidize and damage important substances such as lipids, proteins and nucleic acids in cells. Antioxidants are basically compounds that are good at electromagneticosis. Quickly abandon one of his former electrons so he can break free radicals. In the process, the antioxidant itself becomes a helpless free radical, which does not harm the human body. Here, if another antioxidant is present, the antioxidant that loses the antioxidant power by donating one electron is reduced to the original antioxidant site.

In general, good electronic function is good antioxidant capacity, but not necessarily. Outer leaves extract has a relatively good antioxidant capacity, and the root extracts have good electron donating ability. That is, the extracellular leaf extract with good antioxidant power decomposes free radicals by donating electrons to inhibit free radicals, and the extracellular leaf extract turns into free radicals, which are harmless to the human body, and no longer function as antioxidants. By the way, if the presence of eukaryotic muscle extract with excellent electromagnetism ability than the antioxidant power is present, the extra-leaf extract extracted by donating one electron from the eukaryotic muscle extract is reduced to the original antioxidant site.

On the other hand, the skin soothing sunscreen functional cosmetic composition according to the present invention comprises 0.1 to 30.0% by weight of the extra-foliar extract, and 0.1 to 30.0% by weight of the root extract. Here, if the stone extract is less than 0.1% by weight, the antioxidant effect does not appear, and if it is more than 30.0% by weight, the stability of the formulation of the sunscreen is not maintained, and when the extract is less than 0.1% by weight, electron donating ability This is because the effect does not appear, and the stability of the formulation of the sunscreen is not maintained at 30.0% by weight or more.

Preparation of Jeju-dodol leaf extract and jangjanggeun extract as an active ingredient in the composition of the present invention is as follows. First, the stone leaves and knotweed are washed with purified water, naturally dried in the shade, and then ground using a grinder. The crushed stone leaves and ephedra root were put in ethanol, and extracted and filtered three times. The reason why the ethanol extraction method was chosen over the hot water extraction method or the organic solvent extraction method is that in the hot water extraction method, the active ingredient dissolved in water is small and there is a risk that the active ingredient may be destroyed by the cutting water. This is because side effects are concerned. The filtrate was concentrated under reduced pressure to obtain an extra-dolan ethanol extract and kojangeun ethanol extract.

Whitening leaf extract used in the present invention is known to whiten effect, such as inhibiting melanin production and tyrosinase activity, in the present invention to measure the antioxidant effect for inhibiting the active oxygen caused by ultraviolet exposure of ultrafine titanium dioxide, The present invention was completed by confirming the continuous phototoxic inhibitory effect due to ultrafine titanium dioxide by containing elongated root extract with electron donating ability to show a continuous antioxidant effect.

The functional cosmetic composition of the sunscreen of the present invention may be prepared in a lotion, cream formulation that is conventionally prepared. Hereinafter, preferred examples are provided to help understanding of the present invention. However, the following examples are merely provided to more easily understand the present invention, and the contents of the present invention are not limited by the examples.

In the following examples, the antioxidant effect was confirmed by measuring the superoxide dismutase-like activity, which is the antioxidant effect of the extra-leaf extract and the extract of Escherichia coli. In addition, by measuring the electron donating ability of the extra stone leaf extract and the extract of ephedra root to determine whether it can show a continuous antioxidant effect. Ultrafine particles In the sunscreen formulation containing titanium dioxide, Jeju edible leaf extract and Escherichia coli extract were combined to determine the skin soothing effect of the present invention by measuring the inhibitory effect of phototoxicity.

Example 1 Preparation of Ethanol Extracellular Leaf Extract

The stone leaves are washed with purified water, dried naturally in the shade, and then ground to a size of 2 to 3 cm using a grinder. 1 kg of crushed stone leaves were put in 9 L of ethanol and extracted for 24 hours at 15 to 25 ℃. The extract was filtered using Whatman No. 3 filter paper, and the cooling condenser was concentrated under reduced pressure at 30 to 40 ° C. using an extractor to obtain 40.7 g of ethanol monocotyledonous extract.

Example 2 Preparation of Ethanol Extracts

Except for using ephedra root instead of stone leaves in Preparation Example 1, 38.7 g of ethanol ephedra extract was obtained.

Experiment 1: Antioxidant Effect Measurement

Superoxidease dismutase (SOD) -like activity, a representative measure of antioxidant effect, was measured. SOD-like activity is measured as follows. 3 ml of Tris-HCl buffer solution (pH 8.5) and 0.2 ml of 7.2 mM pyrogallol were added to 0.2 ml of the extrafoliar extract and the root extract of the root extract, respectively, and then vortex mixed for 10 seconds at 25 ° C. After reacting for 10 minutes, 1 ml of 1 M HCl was added to stop the reaction, and the amount of pyrogelol oxidized by absorbance at 420 nm was measured. SOD-like activity was expressed by the difference of oxidized pyrogelol in absorbance of the sample addition group and no addition group.

     SOD-like activity (%) = {1- (sample absorbance / non-addition absorbance)} × 100

The SOD-like activity of the extra-leaf leaves and P. root extracts was 85.7% and 13.5%, respectively, and the extra-leaf leaves showed stronger antioxidant effects than the extracts of the roots.

Experiment 2: Measurement of Electron Donating Ability

    Electron donating ability can be measured by the following method. 1 ml of 0.2 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH) was added to 2 ml of the sample solution, followed by vortex mix for 10 seconds, and reacted for 25 to 30 minutes. The absorbance was measured at 517 nm. The electron donating ability was expressed as a percentage difference in absorbance between the sample addition group and the no addition group.

   Electron donating ability (%) = {1- (sample absorbance / non-addition absorbance)} × 100

The electron donating abilities of the extra-leaf leaves extract and the root extract were 25.7% and 84.4%, respectively. It can be seen that the electron donating ability of the E. coli extract exhibits a strong electron donating ability compared to the extra-leaf extract.

This results in a strong anti-stone leaf extract with antioxidant capacity when the extra-leaf leaf extract and the root extract are combined together, and the root extract extract provides electron donating ability to the extra-leaf leaf extract to provide continuous antioxidant capacity of the extra-leaf leaf extract. It can be seen that the antioxidant system was established.

Example Preparation 3 Preparation of External Skin Sunscreen

    It is an experiment for confirming the skin toxicity of the ultrafine titanium dioxide according to the present invention due to the release of free radicals, that is, the skin safety and the soothing effect of the sunscreen containing crustacean extract and ephedra extract. The basic formulation of the sunscreen was prepared with the following ingredients based on the Sun Protection Factor (SPF) 50. To prepare the sunscreen cream, one aqueous beaker contains water-soluble components such as purified water and glycerin, a polymer, and titanium dioxide, an opaque physical barrier agent, and the mixture is heated to 75 ° C. Other oily beakers are mixed with oil-soluble components such as cyclohexasiloxane, chemical sunscreen and surfactant, and warmed to 75 ° C. The aqueous beaker and the oil beaker are mixed and mixed with a homomixer for 4 minutes at a speed of 3600 rpm. After that, the mixture is cooled by mixing with a paddle mixer and the extract, the fragrance, and the preservative are mixed below 50 ° C. to complete the sunscreen. However, only the content of titanium dioxide, stone extra-leaf extract, ephedra root extract was prepared as shown in the table below.

ingredient
content(%) Comparative Example 1 Comparative Example 2 Comparative Example 3 Example 1 Comparative Example 4  Purified water To 100 To 100 To 100 To 100 To 100  glycerin 5.0 5.0 5.0 5.0 5.0  Butylene Glycol 3.0 3.0 3.0 3.0 3.0  Titanium dioxide (particle size, 2 ㎛) - - - - 4.5  Titanium dioxide (particle size, 200 nm, ultra fine particles) 4.5 4.5 4.5 4.5 4.5  Stone leaf extract - 2.0 - 2.0 -  Root Root Extract - - 2.0 2.0 -  Ethylhexylmethoxycinnamate 5.0 5.0 5.0 5.0 5.0  Ethyl hexyl palmitate 4.0 4.0 4.0 4.0 4.0  Cyclopentasiloxane 3.5 3.5 3.5 3.5 3.5  4-Methylbenzylidene Campus 2.5 2.5 2.5 2.5 2.5  Butyl methoxydibenzoylmethane 1.2 1.2 1.2 1.2 1.2  Neopentylglycoldiheptanoate 1.0 1.0 1.0 1.0 1.0  Ethylhexylsalicylate 1.0 1.0 1.0 1.0 1.0  Octocrylene 0.8 0.8 0.8 0.8 0.8  Cyclohexasiloxane 0.7 0.7 0.7 0.7 0.7  Methyl Methacrylate Crosspolymer 0.2 0.2 0.2 0.2 0.2  Glycolstearate 0.2 0.2 0.2 0.2 0.2  Triceteareth-4phosphate 0.2 0.2 0.2 0.2 0.2  Glyceryl stearate 0.2 0.2 0.2 0.2 0.2 Acrylate / C10-30 Alkylacrylate Crosspolymer 0.1 0.1 0.1 0.1 0.1  Triethanolamine 0.1 0.1 0.1 0.1 0.1  Phenoxyethanol 0.1 0.1 0.1 0.1 0.1  Propylparaben 0.1 0.1 0.1 0.1 0.1  Spices 0.1 0.1 0.1 0.1 0.1

Experiment 3: cumulative skin irritation test by phototoxicity

    Using each of the sunscreens prepared in Preparation Example 3, a total of nine 24 hours accumulates every other day on the upper forearm of 30 healthy males and females (man-15 and woman-15) in their 20s and 40s. Patches were performed and UV-A and UV-B were injected for 3 minutes, respectively, and the effects of UV exposure, free radicals, and phototoxicity were measured. The patching method used a fin chamber (Finn Chamber, Epitest, Ltd, Finland). 15 µl of each of the sunscreens prepared in Preparation Example 3 was added dropwise to the chamber, and then UV-A and UV-B were sequentially injected for 3 minutes, followed by patching. The degree of response that appeared on the skin each time was scored using the following equation.

Average responsiveness = {[response index × responsiveness) / (total number of subjects × highest score)] × 100} / number of tests

At this time, ± 1 point in the reaction, + 2 points, + + gives a score of 4 points, was determined as a safe composition when the average reactivity is less than 3. The results are shown in the table below.

Where reactivity is the reactivity of erythema. That is, when the skin is irritated, the red color is called erythema, and the red color is deeply red, and the erythema responsiveness is increased.

As shown in the above table, the general particle size titanium dioxide of Comparative Example 4 shows a very safe composition of 0.37, but when the ultrafine titanium dioxide of Comparative Example 1 is included, the average reactivity is 2.30, but a safe composition is slightly higher. Skin reactions were noted. To prevent turbidity of titanium dioxide, ultrafine titanium dioxide should be used, resulting in high skin reaction due to phototoxicity.

On the other hand, in Comparative Examples 2 and 3 containing the extra-leaf extract and P. rootis extract alone, the average reactivity was low. This is a result of lack of sustained antioxidant capacity. In the case of the sunscreen of Example 1, which contains a combination of extra-leaf leaves and splendid muscle extracts, the skin calming effect does not occur at all due to the synergistic effect of the antioxidant power of the extra-leaf leaves extract and the electron donating ability of the extracts of the root extracts. The sunscreen could be completed.

Claims (1)

As used in sunscreen functional cosmetics containing ultrafine titanium dioxide having a particle size of 370 nm or less,
It contains 0.1 to 30.0% by weight of the stone extract extracted from the leaves of the dorsal leaves using ethanol and 0.1 to 30.0% by weight of the root extract extracted from the knotweed muscle using ethanol,
A skin calming sunscreen functional cosmetic composition containing a crypt extract and a pomace root extract, characterized in that the root extract of E. coli has an electron donating ability to maintain the antioxidant capacity of the foliar leaf extract.