KR102364260B1 - Cosmetic composition for skin soothing containing complex extract including Gynostemma pentaphyllum, Saponaria officinalis L. and Lonicera Japonica Flower - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a mixed extract of Gynostemma pentaphyllum, Saponaria officinalis, and Lonicera japonica as an active ingredient and, more specifically, to a cosmetic composition which contains a mixed extract of Gynostemma pentaphyllum, Saponaria officinalis and Lonicera japonica to exhibit an excellent skin protection effect, a skin inflammation alleviation effect, a skin irritation alleviation and soothing effect, a moisturizing effect and a skin barrier strengthening effect.

Description

Cosmetic composition for skin soothing containing complex extract including Gynostemma pentaphyllum, Saponaria officinalis L. and Lonicera Japonica Flower

The present invention relates to a cosmetic composition containing as an active ingredient a mixed extract of Dol et al., soap grass, and gold and silver lilies as an active ingredient, and more particularly, it contains a mixed extract of Dol et al. It relates to a cosmetic composition exhibiting a soothing and soothing effect, a moisturizing effect and a skin barrier strengthening effect.

The stratum corneum of the skin is the first barrier between the skin and the external environment and is an important factor in determining the condition of the skin. The stratum corneum of healthy skin contains 15-20% of moisture, and when the moisture falls below 10%, it becomes dry and wrinkles increase due to decreased gloss and elasticity. When defects occur due to extrinsic factors such as penetration of irritants or intrinsic factors such as aging, hormones, and stress, the resistance decreases, resulting in decreased function of the epidermal double lipid membrane and increased sensitivity to stimulants, resulting in sensitive and dry skin. .

Human skin is generally stimulated by external influences such as cosmetics, stress, and air pollution for a long period of time, thereby increasing sensitivity and becoming sensitive skin causing dermatitis. The main symptoms of sensitive skin are redness, dry skin, dermatitis and acne. Among these, redness is a reddening phenomenon of the skin, which is reddened and congested by the expansion of blood vessels, and is the most common skin reaction caused by various internal or external stimuli.

In addition, as the skin barrier function is weakened by stimulation, irritant substances penetrate into the stratum corneum and cause irritation to cause skin inflammation.

Under this background, the present inventors researched to develop a cosmetic with a skin soothing effect, and the mixed extract prepared by mixing stone, soap grass, and gold and silver coins used as conventional cosmetics in a specific ratio improves skin inflammation excellently by its synergistic effect. , has completed the present invention by confirming that it exhibits not only skin irritation relief and soothing effects, but also excellent skin moisturizing and skin barrier strengthening effects.

(0001) Republic of Korea Patent Publication No. 10-2008-0022010 (2008.03.10) (0002) Republic of Korea Patent Publication No. 10-2017-0019830 (2017.02.22) (0003) Republic of Korea Patent Registration No. 10-1216410 (2012.12.21) (0004) Republic of Korea Patent Publication No. 10-2008-0099073 (2008.11.12)

An object of the present invention is to provide a cosmetic composition containing a mixed extract of dol, soap grass, and gold and silver flowers as active ingredients to relieve skin inflammation and irritation caused by external stimuli, and to provide excellent skin protection and skin soothing effects.

Another object of the present invention is to provide a cosmetic composition containing a mixed extract of dol, soap grass, and gold and silver flower as active ingredients, thereby exhibiting an excellent moisturizing effect and skin barrier strengthening effect.

In order to achieve the above object, according to the present invention, there is provided a cosmetic composition containing a mixed extract of stone, soap grass, and gold and silver flower as an active ingredient.

Preferably, the mixed extract is extracted by mixing in a weight ratio of 1~3:0.5~1:1~2, more preferably 2:1:1, respectively.

The mixed extract as an active ingredient is contained in an amount of 0.001 to 20% by weight based on the total weight of the cosmetic composition.

The cosmetic composition is characterized in that it is for relieving skin inflammation and irritation caused by external stimuli or for soothing the skin.

The cosmetic composition is characterized in that it is for moisturizing the skin or for strengthening the skin barrier.

Due to the synergistic effect of the present invention, the mixed extract of stone, soap grass, and gold and silver flowers is excellent in inhibiting skin inflammatory reaction caused by external stimuli, alleviating irritation, moisturizing the skin, and strengthening the skin barrier. It can be usefully used.

Hereinafter, the present invention will be described in more detail.

According to the present invention, there is provided a cosmetic composition containing as an active ingredient a mixed extract of Dol et al.

Gynostemma pentaphyllum is a perennial creeper of the dicotyledonous gourd family. It grows in mountains or forests. The rhizome extends sideways and has white hairs on the nodes, and it grows while tangled, but it also climbs up with tendrils. The leaves are alternate, compound leaves with 5 small leaves, narrow egg-shaped oval or narrow egg-shaped. The small leaf at the tip is 4~8cm long and 2~3cm wide along with the small petiole, has a sharp tip, has fine hairs on the front leaf vein, and has sawteeth on the edge. As a raw material for tea and as a health herb, it is effective for obesity, neuralgia, and high blood pressure. In the present invention, extracts of leaves, stems, or whole plants other than stones are used.

Saponaria officinalis L. is a perennial herb of the dicotyledon family Dianthus family. The rhizome is white and thick, and the leaves are opposite to each other in the shape of a willow leaf. The length of the stem is 30-90 cm. In June, many pale pink or white flowers bloom at the tip of the stem. Used for medicinal purposes. It contains saponin and is used for coughing with sputum. In the present invention, extracts of leaves, stems or herbs of soap grass are used.

Gold and silver flower (金銀花) refers to the flower buds of Lonicera japonica Thunberg or its varieties. Gold and silver coins are used when there is a fever and chest tightness and thirst. It is good for inflammation, so it is effective in discharging boils, poison caused by loose skin, inflammation of organs, and pus. It is also used for dysentery, necrosis of skin tissue due to heat poisoning, and mastitis. It can also be applied to colitis, gastric ulcer, cystitis, sore throat, tonsillitis, bronchitis, conjunctivitis and swelling, high fever caused by mumps, and purulent infections. As pharmacological actions, antibacterial action, anti-inflammatory action, antipyretic action, increase in leukocyte phagocytic action, central nerve excitability action, serum cholesterol lowering effect, and ulcer prevention effect have been reported.

As an active ingredient, the mixed extract of the stone, soap grass, and gold leaf is a hot water extract, or water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, It is a solvent extract with at least one solvent selected from the group consisting of petroleum ether and diethyl ether.

Extraction may be performed by a conventional method. According to one embodiment of the present invention, it is prepared by immersing the stone, soap grass, and gold and silver coins at a stable temperature or extracting them under reflux by heating in an extractor equipped with a cooling condenser, then filtering and concentrating under reduced pressure, and freeze-drying the concentrate under reduced pressure. In addition, it may include a low-temperature aging or high-temperature aging process after performing the extraction process.

At this time, preferably, the mixed extract is prepared by mixing in a weight ratio of 1-3: 0.5-1: 1-2, most preferably 2: 1: 1, respectively, including stone, soap grass, and gold and silver flower. The mixed extract may be prepared by preparing the extracts of Dol et al., soap grass, and gold and silver coins, respectively, and then mixing.

According to the synergistic action of the stone, soap grass, and gold and silver flower mixed extracts of the present invention prepared in this way, the skin inflammatory reaction suppression and irritation relief effect due to external stimuli are excellent, and the skin soothing effect is excellent, and also has an excellent moisturizing effect and skin barrier strengthening effect. was confirmed to represent

In addition to the stones, the mixed extract of soap grass and gold and silver flowers may be added in an amount of 0.001 to 20% by weight based on the total weight of the cosmetic composition. When it is added in an amount of less than 0.001% by weight, the effect is weak, and when it exceeds 20% by weight, the increase in the effect is decreased compared to the increase in the content, which is rather inefficient.

The cosmetic composition of the present invention can be prepared in the form of general emulsified formulations and solubilized formulations. Cosmetics in emulsified formulations include nutritional lotions, creams, and essences, and solubilized cosmetics include softening lotions. In addition, by containing a dermatologically acceptable medium and base in addition to cosmetics, it can be prepared in the form of an adjuvant that can be applied topically and systemically commonly used in the field of dermatology.

In addition, the formulation of the cosmetic composition in the present invention is not limited, but skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, essence, It can be applied in any one formulation selected from the group consisting of pack, soap, shampoo, treatment, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder, and eye shadow.

[Example]

Hereinafter, the present invention will be described in more detail based on the following examples and test examples. However, the following examples are only for illustrating the present invention, and the present invention is not limited by the following examples.

Comparative Example 1: Preparation of extracts from Dol et al.

The dried outgrowth was put in 70% hydrous ethanol corresponding to 10 times the dry weight, and extracted at 60° C. for 6 hours in an extractor equipped with a cooling condenser. This was concentrated at a temperature of 50 to 60 ℃ using a reduced pressure concentrator, and then freeze-dried with a freeze dryer to obtain an extract powder.

Comparative Example 2: Preparation of soap grass extract

The dried soapy grass leaves were placed in 70% aqueous ethanol equivalent to 10 times the dry weight, and extracted at 60° C. for 6 hours in an extractor equipped with a cooling condenser. This was concentrated at a temperature of 50 to 60 ℃ using a reduced pressure concentrator, and then freeze-dried with a freeze dryer to obtain an extract powder.

Comparative Example 3: Preparation of extract of gold and silver flowers

The dried gold and silver coins were placed in 70% aqueous ethanol equivalent to 10 times their dry weight, and extracted at 60° C. for 6 hours in an extractor equipped with a cooling condenser. This was concentrated at a temperature of 50 to 60° C. using a vacuum concentrator, and then freeze-dried with a freeze dryer to obtain an extract powder.

Examples 1 to 6: Preparation of mixed extracts

After the dried stones, soap grass leaves, and gold and silver coins were mixed in the ratio shown in Table 1 below, they were placed in 70% aqueous ethanol equivalent to 10 times the dry weight, and extracted at 60° C. in an extractor equipped with a cooling condenser for 6 hours. This was concentrated at a temperature of 50 to 60° C. using a vacuum concentrator, and then freeze-dried with a freeze dryer to obtain an extract powder.

weight ratio Example One 2 3 4 5 6 other than stone One One One 2 2 3 soap paste 0.5 0.5 One 0.5 One One gold coin One 2 One One One 2

Test Example 1: Confirmation of skin cell protection effect

The skin cytoprotective effect was confirmed by the treatment of the samples prepared in Comparative Examples and Examples, except for stone, soap grass, and gold and silver flower extracts. Human-derived fibroblasts were cultured in a 96-well plate at a concentration of 1×10 ³ cells/well, and 0.2 ml of IMDM medium containing 10% FBS was supplied and cultured for 24 hours in a CO ₂ incubator. Here, 0.1% of each sample of Examples and Comparative Examples dissolved in DMSO was treated, and then UV-B (100mJ/cm ² ) was treated and cultured for 24 hours. Thereafter, MTT assay was performed to determine the viability of the cells. In the MTT assay, 0.1 ml of MTT solution was added to the cultured cells, followed by incubation for 4 hours, then the medium was removed and the formazan formed by adding 0.5 ml of DMSO was measured for absorbance at 570 nm using ELISA. Cell viability (%) was measured by the following formula, and the results are shown in Table 2 below.

Cell viability (%) = [(St-Bo/Bt-Bo)] × 100

Bo: Absorbance at 570 nm of wells in which only the cell culture medium was subjected to color development

Bt: Absorbance at 570 nm of wells subjected to color reaction without sample treatment

St: Absorbance at 570 nm of wells subjected to sample treatment and color reaction

division Treatment concentration (w/v%) Cell viability (%) Example 1 0.1 66.2 Example 2 0.1 65.6 Example 3 0.1 69.3 Example 4 0.1 72.8 Example 5 0.1 76.1 Example 6 0.1 71.2 Comparative Example 1 0.1 58.7 Comparative Example 2 0.1 51.9 Comparative Example 3 0.1 53.0

As can be seen from Table 2 above, it was confirmed that the mixed extracts of Dol et al., Soap Grass, and Gold and Silver in the Examples of the present invention had superior cell protection effects from UV stimulation compared to Comparative Examples, which are extracts of Dol et, Soap grass, and Gold and Silver respectively.

Test Example 2: COX-2 and PGE-2 expression reduction effect by stimulation

In order to examine the effect on the cytokine production of Human Epidermal Keratinocyte (HaCaT), 100 ul of 5×10 ⁵ cells/ml cells were put into a 96 well plate, incubated in a CO ₂ incubator for 48 hours, and then the medium was discarded, After washing the surface with PBS solution, it was replaced with a medium not added with FBS, starvated for 18 hours, and cultured for 24 hours. After incubation, the stimulation factors UV-B (100mJ/cm ² ), LPS (10ng/ml), and SLS (10ppm) were respectively treated to stimulate the cells, and 0.1% of each sample of Examples and Comparative Examples was treated, After culturing for 24 hours, mRNA expression levels were investigated using RT-PCR.

Using the mRNA expression level increased by each of the stimuli as a control, the expression reduction rate was calculated by the following formula, and the results are shown in Table 3.

mRNA expression reduction rate (%) = [1- (sample mRNA expression level / control group mRNA expression level)] × 100

division COX-2 expression reduction rate PGE2 expression reduction rate UV-B LPS SLS UV-B LPS SLS Example 1 16.0 11.0 22.5 17.4 10.2 9.8 Example 2 18.8 15.8 19.0 15.5 9.9 10.3 Example 3 21.4 13.4 25.4 18.6 13.4 11.1 Example 4 25.3 18.1 26.9 21.9 17.7 15.5 Example 5 28.1 21.2 28.2 22.7 18.9 17.0 Example 6 22.3 16.8 24.8 20.3 15.0 12.9 Comparative Example 1 14.4 10.7 14.3 14.1 9.4 8.2 Comparative Example 2 8.6 6.9 10.1 10.4 7.2 4.5 Comparative Example 3 11.0 9.5 13.6 12.7 8.0 6.8 Dexamethasone 27.4 20.5 28.9 23.2 19.1 16.7

As can be seen in Table 3 above, the mixed extracts of Dol et al., Soapgrass, and Gold and Silver flowers of the Examples of the present invention showed the expression of COX-2 and PGE-2, which are inflammatory factors induced by stimulation, compared to the Comparative Example, which is an extract of a single natural product. It was further reduced, and Example 5 showed the most excellent effect.

Test Example 3: NF-kB and TNF-a expression reduction effect by stimulation

In order to examine the effect on the cytokine production of Human Epidermal Keratinocyte (HaCaT), 100 ul of 5×10 ⁵ cells/ml cells were put into a 96 well plate, incubated in a CO ₂ incubator for 48 hours, and then the medium was discarded, After washing the surface with PBS solution, it was replaced with a medium not added with FBS, starvated for 18 hours, and cultured for 24 hours. After incubation, the cells were stimulated by treatment with UV-B (100mJ/cm ² ), LPS (10ng/ml), and SLS (10ppm), and 0.1% of each of the samples of Examples and Comparative Examples were treated, 24 After culturing for a period of time, the cells were examined for mRNA expression levels using RT-PCR.

Using the mRNA expression level increased by each of the stimulatory factors as a control, the expression reduction rate was calculated by the following formula, and the results are shown in Table 4.

mRNA expression reduction rate (%)=[1-(sample mRNA expression level/control mRNA expression level)]×100

division NF-kB expression reduction rate TNF-a expression reduction rate UV-B LPS SLS UV-B LPS SLS Example 1 18.6 10.1 17.3 15.1 13.2 12.0 Example 2 20.8 13.7 14.4 17.2 19.8 14.5 Example 3 22.0 19.4 18.9 19.9 22.6 17.3 Example 4 23.3 20.0 20.9 23.6 26.7 20.5 Example 5 28.4 21.8 22.3 25.8 29.3 21.4 Example 6 24.3 18.7 21.2 23.0 25.4 19.9 Comparative Example 1 16.7 9.4 11.8 13.0 9.3 8.8 Comparative Example 2 11.0 5.0 10.2 10.8 6.7 7.2 Comparative Example 3 14.6 7.5 8.9 7.9 8.0 6.1 Dexamethasone 27.8 20.3 23.9 25.2 31.4 21.7

As can be seen in Table 4 above, the mixed extracts of Dol et al., Soapgrass, and Geumgyeonghwa in the Examples of the present invention showed the expression of inflammatory factors NF-kB and TNF-a induced by stimulation compared to Comparative Example, which is an extract of a single natural product. further reduced.

Test Example 4: Hyaluronic acid production amount measurement

The amount of hyaluronic acid produced by the mixed extracts of stone, soap grass, and gold and silver flowers prepared in Examples of the present invention was measured. HaCaT cells were aliquoted in a 96-well plate at a concentration of 1×10 ⁵ cells/well and cultured, replaced with a medium not added with FBS, starvated for 18 hours, and cultured for 24 hours. HaCaT cells were treated with 0.1% of each sample and cultured for 24 hours. The recovered culture medium was measured for hyaluronic acid (HA) production using an ELSIA kit. The results are shown in Table 5

division HA production (ug/ml) Example 1 30.4 Example 2 33.7 Example 3 36.2 Example 4 40.6 Example 5 43.0 Example 6 38.6 Comparative Example 1 28.3 Comparative Example 2 24.7 Comparative Example 3 21.5 hyaluronic acid 43.4

As can be seen in Table 5 above, the mixed extracts of Dol et al., soap grass, and gold and silver flowers of Examples of the present invention showed superior hyaluronic acid production compared to Comparative Examples, and among them, Example 5 showed the most excellent effect.

Example 7: Preparation of cream containing mixed extracts of Dol et al.

A cream containing the mixed extract of dol, soap grass, and gold and silver flowers of Example 5 was prepared according to a conventional method with the composition and content shown in Table 6 below.

ingredient content (wt%) Control Example 1 Example 7 Mixed extract (Example 5) - 2.0 lipophilic monostearate glycerin 2.0 2.0 stearyl alcohol 2.2 2.2 stearic acid 1.5 1.5 beeswax 1.0 1.0 Polysorbate 60 1.5 1.5 Sorbitan Stearate 0.6 0.6 purified vegetable oil 1.0 1.0 squalane 3.0 3.0 mineral oil 5.0 5.0 trioctanoin 5.0 5.0 dimethicone 1.0 1.0 Sodium Magnesium Silicate 0.1 0.1 glycerin 5.0 5.0 betaine 3.0 3.0 triethanolamine 1.0 1.0 Sodium Hyaluronate 4.0 4.0 preservatives, fragrances, colors a very small amount a very small amount Purified water remaining amount remaining amount Sum 100 100

Test Example 5: Confirmation of skin redness alleviation effect

In order to confirm the skin redness alleviation effect, the formulation of Example 7 and Control Example 1 was used to evaluate the soothing effect of skin redness relief in 20 women who met the test subject selection criteria. Subjects were prohibited from using functional care products that could affect the experimental results.

Before the start of the experiment, the redness of the skin to be measured was photographed, and comparative evaluation was performed after cleansing every morning and evening for 2 weeks. For evaluation, a spectrophotometer (Spectrophotometer CR-2600D, Konica Minolta, Japan) was used.

As a value to measure the soothing effect of redness relief, the a* value indicating skin redness was used for the analysis, and as the measured value decreases compared to before use, it means that there is a calming effect by redness relief. The improvement evaluation of the sedative effect by redness relief was measured using the following formula, and then shown in Table 7.

division Initial (D0) mean 2 weeks (D14) average Improvement (%) Control Example 1 10.92 10.47 4.12 Example 7 11.28 10.22 9.40

As can be seen in Table 7, the composition of Example 7 containing the mixed extract of Dol et al., soap grass, and gold and silver flowers of the present invention exhibited superior skin redness relief effect than the composition of Control Example 1.

Test Example 6: Confirmation of skin barrier function strengthening effect

In order to confirm the skin barrier function strengthening effect, after damaging the skin barrier by using the taping stripping method on the upper arm of a person, each of the creams prepared in Example 7 and Control Example 1 was applied in an area of 5 cm ² After applying to the skin, the degree of strengthening of the barrier function was evaluated by measuring the amount of transdermal water loss (TEWL) after 2 hours, 4 hours, 6 hours, and 8 hours, and evaluating the degree of decrease. The amount of epidermal water loss was measured by Cortex Technology (Denmark) DermaLab Combo. The results are shown in Table 8 below after comparing changes over time based on the amount of moisture loss in the epidermis in the initial damaged barrier state (100%).

division 0 hours 2 hours 4 hours 6 hours 8 hours Control Example 1 100 45 61 75 85 Example 7 100 27 36 48 57

As can be seen in Table 8, as a result of the skin barrier function strengthening test, the cream composition of Example 7 containing the mixed extract of Dol et al., soap grass, and gold and silver flowers of the present invention had superior skin barrier function compared to the composition of Control Example 1 which did not contain it. Reinforcing effect was shown.

Claims (6)

A cosmetic composition for relieving skin irritation and skin irritation containing as active ingredients a mixed extract of Dol et al. delete delete The cosmetic composition for relieving skin irritation and skin irritation according to claim 1, wherein the cosmetic composition has a skin soothing activity. The cosmetic composition for alleviating skin inflammation and skin irritation according to claim 1, wherein the cosmetic composition has a skin moisturizing activity. The cosmetic composition for relieving skin irritation and skin irritation according to claim 1, wherein the cosmetic composition has a skin barrier strengthening activity.