KR20210032972A - 전사인자를 사용하여 단백질 발현을 증가시키는 수단 및 방법 - Google Patents

전사인자를 사용하여 단백질 발현을 증가시키는 수단 및 방법 Download PDF

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KR20210032972A
KR20210032972A KR1020217002744A KR20217002744A KR20210032972A KR 20210032972 A KR20210032972 A KR 20210032972A KR 1020217002744 A KR1020217002744 A KR 1020217002744A KR 20217002744 A KR20217002744 A KR 20217002744A KR 20210032972 A KR20210032972 A KR 20210032972A
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seq
ser
amino acid
acid sequence
transcription factor
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Korean (ko)
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리차드 쟐
조나스 부가드
크리스틴 바우만
디타르드 매타노비슈
브라이기트 가세르
Original Assignee
베링거 인겔하임 에르체파우 게엠베하 운트 코 카게
발리도겐 게엠베하
론자 리미티드
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Publication of KR20210032972A publication Critical patent/KR20210032972A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag

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  • Life Sciences & Earth Sciences (AREA)
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  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
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  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Peptides Or Proteins (AREA)
KR1020217002744A 2018-06-27 2019-06-27 전사인자를 사용하여 단백질 발현을 증가시키는 수단 및 방법 Pending KR20210032972A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP18180164.8 2018-06-27
EP18180164 2018-06-27
PCT/EP2019/067133 WO2020002494A1 (en) 2018-06-27 2019-06-27 Means and methods for increased protein expression by use of transcription factors

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KR20210032972A true KR20210032972A (ko) 2021-03-25

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KR1020217002744A Pending KR20210032972A (ko) 2018-06-27 2019-06-27 전사인자를 사용하여 단백질 발현을 증가시키는 수단 및 방법

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Country Link
US (1) US20210269811A1 (https=)
EP (1) EP3814491A1 (https=)
JP (2) JP7645076B2 (https=)
KR (1) KR20210032972A (https=)
CN (1) CN112955547B (https=)
AU (1) AU2019294515B2 (https=)
CA (1) CA3103988A1 (https=)
SG (1) SG11202012529VA (https=)
WO (1) WO2020002494A1 (https=)

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CA3175683A1 (en) * 2020-04-29 2021-11-04 Xueyang FENG Compositions and methods for enhancing recombinant biosynthesis of cannabinoids
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CN119799530B (zh) * 2024-12-31 2025-12-16 溧阳市优甜生物科技有限公司 重组酵母细胞及其在生产索马甜中的用途
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CN112955547A (zh) 2021-06-11
JP2021528985A (ja) 2021-10-28
EP3814491A1 (en) 2021-05-05
JP2025024050A (ja) 2025-02-19
SG11202012529VA (en) 2021-01-28
WO2020002494A1 (en) 2020-01-02
US20210269811A1 (en) 2021-09-02
CN112955547B (zh) 2024-10-25
AU2019294515A1 (en) 2021-01-14
JP7645076B2 (ja) 2025-03-13
AU2019294515B2 (en) 2025-10-09
CA3103988A1 (en) 2020-01-02

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