CN112955547B - 通过使用转录因子来增加蛋白质表达的手段和方法 - Google Patents

通过使用转录因子来增加蛋白质表达的手段和方法 Download PDF

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CN112955547B
CN112955547B CN201980056449.0A CN201980056449A CN112955547B CN 112955547 B CN112955547 B CN 112955547B CN 201980056449 A CN201980056449 A CN 201980056449A CN 112955547 B CN112955547 B CN 112955547B
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host cell
seq
ser
amino acid
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CN112955547A (zh
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R·察尔
J·博加德
K·鲍曼
D·玛塔诺维克
B·加塞尔
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Valladogan Co ltd
Boehringer Ingelheim RCV GmbH and Co KG
Lonza AG
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Valladogan Co ltd
Boehringer Ingelheim RCV GmbH and Co KG
Lonza AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Peptides Or Proteins (AREA)
CN201980056449.0A 2018-06-27 2019-06-27 通过使用转录因子来增加蛋白质表达的手段和方法 Active CN112955547B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP18180164.8 2018-06-27
EP18180164 2018-06-27
PCT/EP2019/067133 WO2020002494A1 (en) 2018-06-27 2019-06-27 Means and methods for increased protein expression by use of transcription factors

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CN112955547A CN112955547A (zh) 2021-06-11
CN112955547B true CN112955547B (zh) 2024-10-25

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US (1) US20210269811A1 (https=)
EP (1) EP3814491A1 (https=)
JP (2) JP7645076B2 (https=)
KR (1) KR20210032972A (https=)
CN (1) CN112955547B (https=)
AU (1) AU2019294515B2 (https=)
CA (1) CA3103988A1 (https=)
SG (1) SG11202012529VA (https=)
WO (1) WO2020002494A1 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018119142A1 (en) 2016-12-21 2018-06-28 Amgen Inc. Anti-tnf alpha antibody formulations
BR112022015325A2 (pt) * 2020-02-04 2022-09-27 Clara Foods Co Sistemas e métodos para alta produção de microganismos recombinantes e uso dos mesmos
ES3034258T3 (en) 2020-04-01 2025-08-14 Lonza Ag Helper factors for expressing proteins in yeast
CA3175683A1 (en) * 2020-04-29 2021-11-04 Xueyang FENG Compositions and methods for enhancing recombinant biosynthesis of cannabinoids
CN114657190B (zh) * 2022-04-06 2023-08-29 暨南大学 Msn2p作为负调控因子在提高宿主细胞中蛋白表达中的应用
CN114836461B (zh) * 2022-05-31 2024-03-29 华南理工大学 表达胶原蛋白酶的重组质粒、酵母菌株及其发酵培养基和发酵培养方法
CN115725632B (zh) * 2022-07-26 2023-12-22 深圳技术大学 一种Aomsn2过表达米曲霉工程菌及其构建方法与应用
EP4634389A1 (en) * 2022-12-16 2025-10-22 Boehringer Ingelheim RCV GmbH & Co KG Means and methods for increased protein expression by use of a combination of transport proteins and either chaperones or transcription factors
WO2025129656A1 (zh) * 2023-12-22 2025-06-26 中国科学院深圳先进技术研究院 一种模块化正交真核转录因子模型构建方法
CN117777275B (zh) * 2024-02-23 2024-05-31 北京国科星联科技有限公司 一种促进人乳铁蛋白在马克斯克鲁维酵母中分泌表达的方法
CN119220426B (zh) * 2024-12-05 2025-04-25 通化安睿特生物制药股份有限公司 一种重组毕赤酵母工程菌及其构建方法和应用
CN119799530B (zh) * 2024-12-31 2025-12-16 溧阳市优甜生物科技有限公司 重组酵母细胞及其在生产索马甜中的用途
CN120249088B (zh) * 2025-06-05 2025-09-09 上海欣瑞特生物医药技术有限公司 一种用于制备重组犬白蛋白的重组工程菌及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101679992A (zh) * 2007-04-20 2010-03-24 波利门科学生物免疫研究有限公司 酵母菌表达系统
CN106604986A (zh) * 2014-04-17 2017-04-26 贝林格尔·英格海姆Rcv两合公司 用于表达目标蛋白的重组宿主细胞
CN106661541A (zh) * 2014-04-17 2017-05-10 贝林格尔·英格海姆Rcv两合公司 被工程化为过表达辅助蛋白的重组宿主细胞

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4601893A (en) 1984-02-08 1986-07-22 Pfizer Inc. Laminate device for controlled and prolonged release of substances to an ambient environment and method of use
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
DE4027453A1 (de) 1990-08-30 1992-03-05 Degussa Neue plasmide aus corynebacterium glutamicum und davon abgeleitete plasmidvektoren
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
DE4440118C1 (de) 1994-11-11 1995-11-09 Forschungszentrum Juelich Gmbh Die Genexpression in coryneformen Bakterien regulierende DNA
JPH10229891A (ja) 1997-02-20 1998-09-02 Mitsubishi Rayon Co Ltd マロン酸誘導体の製造法
GB9704162D0 (en) 1997-02-28 1997-04-16 Medical Res Council Assay methods and means
US6949356B1 (en) 1999-10-20 2005-09-27 Microbia, Inc. Methods for improving secondary metabolite production in fungi
EP1373470B1 (en) * 2001-02-20 2013-04-24 Intrexon Corporation Novel substitution mutant receptors and their use in a nuclear receptor-based inducible gene expression system
JP2003000240A (ja) 2001-06-22 2003-01-07 National Research Inst Of Brewing 麹菌の固体培養発現遺伝子を制御する転写因子をコードするｄｎａ
WO2003012036A2 (en) 2001-07-27 2003-02-13 The Government Of The United States Of America As Represented By The Secretary Of Health And Human Services Systems for in vivo site-directed mutagenesis using oligonucleotides
EP1680447A2 (en) * 2003-10-24 2006-07-19 Istituto Di Ricerche Di Biologia Molecolare P. Angeletti S.P.A. Orthogonal gene switches
US20050130306A1 (en) * 2003-12-10 2005-06-16 Richard Voellmy Viral vectors whose replication and, optionally, passenger gene are controlled by a gene switch activated by heat in the presence or absence of a small molecule regulator
SI22056A (sl) * 2005-05-05 2006-12-31 Kemijski Institut Visoko-produkcijski rekombinantni sevi kvasovk s spremenjeno galaktozno regulacijo transkripcije
CA2644273A1 (en) * 2006-04-05 2008-03-27 Metanomics Gmbh Process for the production of a fine chemical
WO2009058956A1 (en) * 2007-11-01 2009-05-07 Danisco Us, Inc. Signal sequences and co-expressed chaperones for improving protein production in a host cell
KR20100117465A (ko) * 2009-04-24 2010-11-03 삼성전자주식회사 호스트셀의 알코올 내성을 증대시키는 단리된 폴리뉴클레오티드, 이를 포함하는 벡터, 호스트셀 및 이를 이용한 알코올의 생산방법
EP2258854A1 (en) 2009-05-20 2010-12-08 FH Campus Wien Eukaryotic host cell comprising an expression enhancer
JP5585952B2 (ja) 2009-12-08 2014-09-10 独立行政法人酒類総合研究所 エタノールの製造方法
WO2011159153A2 (en) * 2010-06-15 2011-12-22 Universiteit Utrecht Holding B.V. Inducible promoter and its use
CN102643852B (zh) * 2011-02-28 2015-04-08 华东理工大学 光可控的基因表达系统
JP6295512B2 (ja) 2012-03-15 2018-03-20 株式会社豊田中央研究所 酵母における外来遺伝子の発現産物の生産方法、酵母における発現調節剤及びその利用
CN104031854B (zh) * 2014-06-20 2017-02-22 广西科学院 一株提高对乙醇耐受性的酿酒酵母基因工程菌株及其构建方法
KR102287810B1 (ko) * 2014-07-28 2021-08-09 삼성전자주식회사 Msn2의 활성이 증가되도록 유전적으로 조작된, 스트레스 내성을 갖는 효모 세포 및 그를 이용하여 락테이트를 생산하는 방법
EP3180440A4 (en) * 2014-08-11 2018-01-10 Butamax Advanced Biofuels LLC Yeast preparations and methods of making the same
EP3334751A1 (en) * 2015-08-13 2018-06-20 Glykos Finland Oy Regulatory protein deficient trichoderma cells and methods of use thereof
US12060394B2 (en) * 2016-04-28 2024-08-13 The Trustees Of Dartmouth College Nucleic acid constructs for co-expression of chimeric antigen receptor and transcription factor, cells containing and therapeutic use thereof
CN107267529B (zh) * 2017-07-20 2019-09-27 昆明理工大学 一种锌指蛋白转录因子基因RkMSN4及其应用

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101679992A (zh) * 2007-04-20 2010-03-24 波利门科学生物免疫研究有限公司 酵母菌表达系统
CN106604986A (zh) * 2014-04-17 2017-04-26 贝林格尔·英格海姆Rcv两合公司 用于表达目标蛋白的重组宿主细胞
CN106661541A (zh) * 2014-04-17 2017-05-10 贝林格尔·英格海姆Rcv两合公司 被工程化为过表达辅助蛋白的重组宿主细胞

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ACCESSION No. ANZ75234.1 BA75_02431T0 [Komagataella pastoris];Love,K.R. 等;《GenBank数据库》;"FEATURES "部分 *
Protein folding and secretion: mechanistic insights advancing recombinant protein production in S. cerevisiae;Carissa L. Young 等;《Curr Opin Biotechnol.》;摘要，第2页第4段至第4页末段 *

Also Published As

Publication number Publication date
CN112955547A (zh) 2021-06-11
KR20210032972A (ko) 2021-03-25
JP2021528985A (ja) 2021-10-28
EP3814491A1 (en) 2021-05-05
JP2025024050A (ja) 2025-02-19
SG11202012529VA (en) 2021-01-28
WO2020002494A1 (en) 2020-01-02
US20210269811A1 (en) 2021-09-02
AU2019294515A1 (en) 2021-01-14
JP7645076B2 (ja) 2025-03-13
AU2019294515B2 (en) 2025-10-09
CA3103988A1 (en) 2020-01-02

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