CA3103988A1 - Means and methods for increased protein expression by use of transcription factors - Google Patents

Means and methods for increased protein expression by use of transcription factors Download PDF

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Publication number
CA3103988A1
CA3103988A1 CA3103988A CA3103988A CA3103988A1 CA 3103988 A1 CA3103988 A1 CA 3103988A1 CA 3103988 A CA3103988 A CA 3103988A CA 3103988 A CA3103988 A CA 3103988A CA 3103988 A1 CA3103988 A1 CA 3103988A1
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CA
Canada
Prior art keywords
seq
transcription factor
amino acid
host cell
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3103988A
Other languages
English (en)
French (fr)
Inventor
Richard ZAHRL
Jonas BURGARD
Kristin BAUMANN
Diethard Mattanovich
Brigitte Gasser
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim RCV GmbH and Co KG
Validogen GmbH
Lonza AG
Original Assignee
Boehringer Ingelheim RCV GmbH and Co KG
Validogen GmbH
Lonza AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim RCV GmbH and Co KG, Validogen GmbH, Lonza AG filed Critical Boehringer Ingelheim RCV GmbH and Co KG
Publication of CA3103988A1 publication Critical patent/CA3103988A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Peptides Or Proteins (AREA)
CA3103988A 2018-06-27 2019-06-27 Means and methods for increased protein expression by use of transcription factors Pending CA3103988A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP18180164.8 2018-06-27
EP18180164 2018-06-27
PCT/EP2019/067133 WO2020002494A1 (en) 2018-06-27 2019-06-27 Means and methods for increased protein expression by use of transcription factors

Publications (1)

Publication Number Publication Date
CA3103988A1 true CA3103988A1 (en) 2020-01-02

Family

ID=62916411

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3103988A Pending CA3103988A1 (en) 2018-06-27 2019-06-27 Means and methods for increased protein expression by use of transcription factors

Country Status (9)

Country Link
US (1) US20210269811A1 (https=)
EP (1) EP3814491A1 (https=)
JP (2) JP7645076B2 (https=)
KR (1) KR20210032972A (https=)
CN (1) CN112955547B (https=)
AU (1) AU2019294515B2 (https=)
CA (1) CA3103988A1 (https=)
SG (1) SG11202012529VA (https=)
WO (1) WO2020002494A1 (https=)

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ES3034258T3 (en) 2020-04-01 2025-08-14 Lonza Ag Helper factors for expressing proteins in yeast
CA3175683A1 (en) * 2020-04-29 2021-11-04 Xueyang FENG Compositions and methods for enhancing recombinant biosynthesis of cannabinoids
CN114657190B (zh) * 2022-04-06 2023-08-29 暨南大学 Msn2p作为负调控因子在提高宿主细胞中蛋白表达中的应用
CN114836461B (zh) * 2022-05-31 2024-03-29 华南理工大学 表达胶原蛋白酶的重组质粒、酵母菌株及其发酵培养基和发酵培养方法
CN115725632B (zh) * 2022-07-26 2023-12-22 深圳技术大学 一种Aomsn2过表达米曲霉工程菌及其构建方法与应用
EP4634389A1 (en) * 2022-12-16 2025-10-22 Boehringer Ingelheim RCV GmbH & Co KG Means and methods for increased protein expression by use of a combination of transport proteins and either chaperones or transcription factors
WO2025129656A1 (zh) * 2023-12-22 2025-06-26 中国科学院深圳先进技术研究院 一种模块化正交真核转录因子模型构建方法
CN117777275B (zh) * 2024-02-23 2024-05-31 北京国科星联科技有限公司 一种促进人乳铁蛋白在马克斯克鲁维酵母中分泌表达的方法
CN119220426B (zh) * 2024-12-05 2025-04-25 通化安睿特生物制药股份有限公司 一种重组毕赤酵母工程菌及其构建方法和应用
CN119799530B (zh) * 2024-12-31 2025-12-16 溧阳市优甜生物科技有限公司 重组酵母细胞及其在生产索马甜中的用途
CN120249088B (zh) * 2025-06-05 2025-09-09 上海欣瑞特生物医药技术有限公司 一种用于制备重组犬白蛋白的重组工程菌及其应用

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Also Published As

Publication number Publication date
CN112955547A (zh) 2021-06-11
KR20210032972A (ko) 2021-03-25
JP2021528985A (ja) 2021-10-28
EP3814491A1 (en) 2021-05-05
JP2025024050A (ja) 2025-02-19
SG11202012529VA (en) 2021-01-28
WO2020002494A1 (en) 2020-01-02
US20210269811A1 (en) 2021-09-02
CN112955547B (zh) 2024-10-25
AU2019294515A1 (en) 2021-01-14
JP7645076B2 (ja) 2025-03-13
AU2019294515B2 (en) 2025-10-09

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