JP7645076B2 - 転写因子の使用によってタンパク質発現を増加させるための手段及び方法 - Google Patents

転写因子の使用によってタンパク質発現を増加させるための手段及び方法 Download PDF

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JP7645076B2
JP7645076B2 JP2020573043A JP2020573043A JP7645076B2 JP 7645076 B2 JP7645076 B2 JP 7645076B2 JP 2020573043 A JP2020573043 A JP 2020573043A JP 2020573043 A JP2020573043 A JP 2020573043A JP 7645076 B2 JP7645076 B2 JP 7645076B2
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protein
host cell
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amino acid
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ザール，リチャード
バーガード，ヨーナス
バウマン，クリスティン
マッタノビッチ，ディートハルト
ガッセル，ブリジット
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Validogen GmbH
Lonza AG
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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JP2020573043A 2018-06-27 2019-06-27 転写因子の使用によってタンパク質発現を増加させるための手段及び方法 Active JP7645076B2 (ja)

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PCT/EP2019/067133 WO2020002494A1 (en) 2018-06-27 2019-06-27 Means and methods for increased protein expression by use of transcription factors

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JP2024196277A Pending JP2025024050A (ja) 2018-06-27 2024-11-08 転写因子の使用によってタンパク質発現を増加させるための手段及び方法

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WO2018119142A1 (en) 2016-12-21 2018-06-28 Amgen Inc. Anti-tnf alpha antibody formulations
BR112022015325A2 (pt) * 2020-02-04 2022-09-27 Clara Foods Co Sistemas e métodos para alta produção de microganismos recombinantes e uso dos mesmos
ES3034258T3 (en) 2020-04-01 2025-08-14 Lonza Ag Helper factors for expressing proteins in yeast
CA3175683A1 (en) * 2020-04-29 2021-11-04 Xueyang FENG Compositions and methods for enhancing recombinant biosynthesis of cannabinoids
CN114657190B (zh) * 2022-04-06 2023-08-29 暨南大学 Msn2p作为负调控因子在提高宿主细胞中蛋白表达中的应用
CN114836461B (zh) * 2022-05-31 2024-03-29 华南理工大学 表达胶原蛋白酶的重组质粒、酵母菌株及其发酵培养基和发酵培养方法
CN115725632B (zh) * 2022-07-26 2023-12-22 深圳技术大学 一种Aomsn2过表达米曲霉工程菌及其构建方法与应用
EP4634389A1 (en) * 2022-12-16 2025-10-22 Boehringer Ingelheim RCV GmbH & Co KG Means and methods for increased protein expression by use of a combination of transport proteins and either chaperones or transcription factors
WO2025129656A1 (zh) * 2023-12-22 2025-06-26 中国科学院深圳先进技术研究院 一种模块化正交真核转录因子模型构建方法
CN117777275B (zh) * 2024-02-23 2024-05-31 北京国科星联科技有限公司 一种促进人乳铁蛋白在马克斯克鲁维酵母中分泌表达的方法
CN119220426B (zh) * 2024-12-05 2025-04-25 通化安睿特生物制药股份有限公司 一种重组毕赤酵母工程菌及其构建方法和应用
CN119799530B (zh) * 2024-12-31 2025-12-16 溧阳市优甜生物科技有限公司 重组酵母细胞及其在生产索马甜中的用途
CN120249088B (zh) * 2025-06-05 2025-09-09 上海欣瑞特生物医药技术有限公司 一种用于制备重组犬白蛋白的重组工程菌及其应用

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CN112955547A (zh) 2021-06-11
KR20210032972A (ko) 2021-03-25
JP2021528985A (ja) 2021-10-28
EP3814491A1 (en) 2021-05-05
JP2025024050A (ja) 2025-02-19
SG11202012529VA (en) 2021-01-28
WO2020002494A1 (en) 2020-01-02
US20210269811A1 (en) 2021-09-02
CN112955547B (zh) 2024-10-25
AU2019294515A1 (en) 2021-01-14
AU2019294515B2 (en) 2025-10-09
CA3103988A1 (en) 2020-01-02

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