KR20200146043A - 간외 담도계로부터의 다분화능 줄기 세포 및 이의 분리 방법 - Google Patents

간외 담도계로부터의 다분화능 줄기 세포 및 이의 분리 방법 Download PDF

Info

Publication number
KR20200146043A
KR20200146043A KR1020207036724A KR20207036724A KR20200146043A KR 20200146043 A KR20200146043 A KR 20200146043A KR 1020207036724 A KR1020207036724 A KR 1020207036724A KR 20207036724 A KR20207036724 A KR 20207036724A KR 20200146043 A KR20200146043 A KR 20200146043A
Authority
KR
South Korea
Prior art keywords
cells
stem
progenitor
lineage
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
KR1020207036724A
Other languages
English (en)
Korean (ko)
Inventor
롤라 엠. 레이드
윤팡 왕
빈첸초 카르디날레
에우제니오 가우디오
귀도 카르피노
도메니코 알바로
카이-빈 추이
Original Assignee
더 유니버시티 오브 노쓰 캐롤라이나 엣 채플 힐
사피엔자 유니버시타 디 로마
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=43922533&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=KR20200146043(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 더 유니버시티 오브 노쓰 캐롤라이나 엣 채플 힐, 사피엔자 유니버시타 디 로마 filed Critical 더 유니버시티 오브 노쓰 캐롤라이나 엣 채플 힐
Publication of KR20200146043A publication Critical patent/KR20200146043A/ko
Ceased legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/413—Gall bladder; Bile
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • C12N5/068—Stem cells; Progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/22—Zinc; Zn chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/335—Glucagon; Glucagon-like peptide [GLP]; Exendin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/80—Hyaluronan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2537/00—Supports and/or coatings for cell culture characterised by physical or chemical treatment
    • C12N2537/10—Cross-linking

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nutrition Science (AREA)
  • Physiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
KR1020207036724A 2009-10-30 2010-10-28 간외 담도계로부터의 다분화능 줄기 세포 및 이의 분리 방법 Ceased KR20200146043A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25684609P 2009-10-30 2009-10-30
US61/256,846 2009-10-30
PCT/US2010/054450 WO2011053690A1 (en) 2009-10-30 2010-10-28 Multipotent stem cells from the extrahepatic billary tree and methods of isolating same

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020197015956A Division KR20190065476A (ko) 2009-10-30 2010-10-28 간외 담도계로부터의 다분화능 줄기 세포 및 이의 분리 방법

Publications (1)

Publication Number Publication Date
KR20200146043A true KR20200146043A (ko) 2020-12-31

Family

ID=43922533

Family Applications (4)

Application Number Title Priority Date Filing Date
KR1020207036724A Ceased KR20200146043A (ko) 2009-10-30 2010-10-28 간외 담도계로부터의 다분화능 줄기 세포 및 이의 분리 방법
KR1020127013498A Ceased KR20120117758A (ko) 2009-10-30 2010-10-28 간외 담도계로부터의 다분화능 줄기 세포 및 이의 분리 방법
KR1020187013924A Expired - Fee Related KR102016870B1 (ko) 2009-10-30 2010-10-28 간외 담도계로부터의 다분화능 줄기 세포 및 이의 분리 방법
KR1020197015956A Ceased KR20190065476A (ko) 2009-10-30 2010-10-28 간외 담도계로부터의 다분화능 줄기 세포 및 이의 분리 방법

Family Applications After (3)

Application Number Title Priority Date Filing Date
KR1020127013498A Ceased KR20120117758A (ko) 2009-10-30 2010-10-28 간외 담도계로부터의 다분화능 줄기 세포 및 이의 분리 방법
KR1020187013924A Expired - Fee Related KR102016870B1 (ko) 2009-10-30 2010-10-28 간외 담도계로부터의 다분화능 줄기 세포 및 이의 분리 방법
KR1020197015956A Ceased KR20190065476A (ko) 2009-10-30 2010-10-28 간외 담도계로부터의 다분화능 줄기 세포 및 이의 분리 방법

Country Status (20)

Country Link
US (1) US20110135610A1 (cg-RX-API-DMAC7.html)
EP (2) EP3725874A1 (cg-RX-API-DMAC7.html)
JP (2) JP5936547B2 (cg-RX-API-DMAC7.html)
KR (4) KR20200146043A (cg-RX-API-DMAC7.html)
CN (2) CN102918145B (cg-RX-API-DMAC7.html)
AR (2) AR078849A1 (cg-RX-API-DMAC7.html)
AU (1) AU2016210783B2 (cg-RX-API-DMAC7.html)
BR (1) BR112012009848B1 (cg-RX-API-DMAC7.html)
CA (2) CA2779144C (cg-RX-API-DMAC7.html)
CL (1) CL2012001117A1 (cg-RX-API-DMAC7.html)
CR (1) CR20120254A (cg-RX-API-DMAC7.html)
ES (1) ES2819028T3 (cg-RX-API-DMAC7.html)
IL (2) IL219454A (cg-RX-API-DMAC7.html)
MX (1) MX2012005087A (cg-RX-API-DMAC7.html)
NZ (3) NZ709124A (cg-RX-API-DMAC7.html)
PH (1) PH12012500845A1 (cg-RX-API-DMAC7.html)
RU (2) RU2756584C2 (cg-RX-API-DMAC7.html)
SG (2) SG10201913006XA (cg-RX-API-DMAC7.html)
TW (3) TWI641688B (cg-RX-API-DMAC7.html)
WO (1) WO2011053690A1 (cg-RX-API-DMAC7.html)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2819028T3 (es) * 2009-10-30 2021-04-14 Univ North Carolina Chapel Hill Células madre multipotentes del árbol biliar extrahepático y métodos para su aislamiento
WO2012178215A1 (en) 2011-06-23 2012-12-27 The Children's Hospital Of Philadelphia Self-renewing endodermal progenitor lines generated from human pluripotent stem cells and methods of use thereof
US9533013B2 (en) 2013-03-13 2017-01-03 University Of North Carolina At Chapel Hill Method of treating pancreatic and liver conditions by endoscopic-mediated (or laparoscopic-mediated) transplantation of stem cells into/onto bile duct walls of particular regions of the biliary tree
CN103266084B (zh) * 2013-05-27 2015-03-04 刘卫辉 富集干细胞的三步分离法
CN106461676B (zh) * 2014-03-17 2019-07-05 新加坡科技研究局 基于核因子κB易位预测化合物的毒性的方法
GB201507894D0 (en) * 2015-05-08 2015-06-24 Imagen Biotech Ltd Personalised media
GB201510950D0 (en) * 2015-06-22 2015-08-05 Cambridge Entpr Ltd In vitro Production of Cholangiocytes
US10683486B2 (en) * 2015-10-30 2020-06-16 Biolamina Ab Methods for producing hepatocytes
GB201521179D0 (en) 2015-12-01 2016-01-13 Univ Leuven Kath Methods for differentiating cells into hepatic stellate cells
CN105807053B (zh) * 2016-04-15 2019-04-02 苏州药明康德新药开发股份有限公司 一种肿瘤解离试剂在流式细胞检测中的应用
US10767164B2 (en) 2017-03-30 2020-09-08 The Research Foundation For The State University Of New York Microenvironments for self-assembly of islet organoids from stem cells differentiation
KR20190141168A (ko) * 2017-04-06 2019-12-23 더 유니버시티 오브 노쓰 캐롤라이나 엣 채플 힐 동결보존 방법
CA3112650A1 (en) * 2018-03-29 2019-10-03 The University Of North Carolina At Chapel Hill Stem/progenitor cells from duodenal brunner's glands and methods of isolating and using them
CN112126618B (zh) * 2019-06-24 2023-09-12 上海拜羡生物科技有限公司 一种人胆囊干细胞的获取和长期体外培养的方法
CN113388569B (zh) * 2020-08-28 2022-05-17 广东乾晖生物科技有限公司 肝脏类器官的制备方法
BR112023025612A2 (pt) * 2021-06-30 2024-02-27 Polbionica Sp Z O O Meio para armazenar ilhotas pancreáticas isoladas, e, método de armazenamento de ilhotas pancreáticas isoladas
CN116478904A (zh) * 2022-01-17 2023-07-25 香港中文大学 肝样细胞和人工肝组织的制备方法以及应用
CN115975913B (zh) * 2023-02-03 2024-06-04 北京中医药大学深圳医院(龙岗) 一种利用多能干细胞诱导分化胆囊祖细胞的方法

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3646197A (en) * 1969-05-14 1972-02-29 Zaidan Hojin Biseibutsu Novel process for removing copper from copper-containing bleomycin
US6069005A (en) * 1991-08-07 2000-05-30 Albert Einstein College Of Medicine Of Yeshwa University Hapatoblasts and method of isolating same
US5660207A (en) * 1994-12-29 1997-08-26 Tylan General, Inc. Flow controller, parts of flow controller, and related method
US5861313A (en) * 1995-06-07 1999-01-19 Ontogeny, Inc. Method of isolating bile duct progenitor cells
GB9605808D0 (en) * 1996-03-20 1996-05-22 Isis Innovation CFTR gene regulator
US5830850A (en) * 1996-08-28 1998-11-03 Mount Sinai School Of Medicine Of The City Of New York Methods for the treatment of bone resorption disorders, including osteoporosis
CA2877233A1 (en) * 1999-01-19 2000-07-27 Lola M. Reid Human liver progenitors
US7456017B2 (en) * 1999-10-01 2008-11-25 University Of North Carolina At Chapel Hill Processes for clonal growth of hepatic progenitor cells
EP1285057B1 (en) * 2000-04-28 2006-05-31 Children's Medical Center Corporation Isolation of mesenchymal stem cells and use thereof
US6828145B2 (en) * 2000-05-10 2004-12-07 Cedars-Sinai Medical Center Method for the isolation of stem cells by immuno-labeling with HLA/MHC gene product marker
JP3602038B2 (ja) * 2000-07-24 2004-12-15 株式会社日立グローバルストレージテクノロジーズ 磁気ヘッドおよび磁気記録再生装置
DE10064804C2 (de) * 2000-12-22 2003-03-20 Schott Glas Alkalifreie Aluminoborosilicatgläser und ihre Verwendung
AU2002340897A1 (en) 2001-09-12 2003-03-24 Develogen Aktiengesellschaft Fur Entwicklungsbiologische Forschung A method for isolating, culturing and differentiating intestinal stem cells for therapeutic use
US7150990B2 (en) * 2002-03-06 2006-12-19 Reprocell, Inc. Self-renewing pluripotent hepatic stem cells
HUE032238T2 (en) * 2002-03-15 2017-09-28 Univ North Carolina Chapel Hill Primitive and proximal liver stem cells
WO2004071464A2 (en) * 2003-02-12 2004-08-26 Johns Hopkins University School Of Medicine Diagnostic application of differentially-expressed genes in lympho-hematopoietic stem cells
DE10336152B4 (de) * 2003-08-06 2007-02-15 Nephrogen LLC, Salt Lake City Aufreinigungsverfahren für humane mesenchymale Stammzellen
US8119774B2 (en) * 2003-10-09 2012-02-21 Martin Pera Cell marker for hepatic and pancreatic stem cells and progenitor cells
JP2007519397A (ja) * 2003-10-28 2007-07-19 カリタス セント エリザベス メディカル センター オブ ボストン インコーポレイテッド 新規多能性幹細胞およびその使用
US7081582B2 (en) * 2004-06-30 2006-07-25 Microsoft Corporation System and method for aligning and mixing songs of arbitrary genres
JP4824036B2 (ja) 2004-12-23 2011-11-24 ヴィアサイト，インコーポレイテッド 胚体内胚葉細胞の増殖
JP4773777B2 (ja) * 2005-08-30 2011-09-14 グローバル・オーエルイーディー・テクノロジー・リミテッド・ライアビリティ・カンパニー アクティブマトリクス型表示装置
CA2625883A1 (en) * 2005-10-14 2007-04-26 Regents Of The University Of Minnesota Differentiation of non-embryonic stem cells to cells having a pancreatic phenotype
RU2008124158A (ru) * 2005-11-16 2010-01-10 Юниверсити Оф Норт Каролина Эт Чепел Хилл (Us) Внеклеточные матриксные компоненты для экспансии и дифференцировки гепатических клеток-предшественников
WO2008063675A2 (en) * 2006-11-24 2008-05-29 Regents Of The University Of Minnesota Endodermal progenitor cells
CN101855340A (zh) * 2007-03-06 2010-10-06 北卡罗来纳大学查珀尔希尔分校 用于细胞维持、扩增和/或分化的透明质酸、其他基质成分、激素和生长因子的复合物
JP2008307007A (ja) * 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
TW200916581A (en) * 2007-06-15 2009-04-16 Univ North Carolina Paracrine signals from mesenchymal feeder cells and regulating expansion and differentiation of hepatic progenitors using same
RU2473685C2 (ru) * 2007-07-31 2013-01-27 Лайфскен, Инк. Дифференцировка человеческих эмбриональных стволовых клеток
ES2819028T3 (es) * 2009-10-30 2021-04-14 Univ North Carolina Chapel Hill Células madre multipotentes del árbol biliar extrahepático y métodos para su aislamiento
US10135700B2 (en) 2014-03-10 2018-11-20 Softphone SRL Enterprise application integration on client computing devices
US10387547B2 (en) 2014-09-09 2019-08-20 Intentional Software Corporation Layout engine for creating a visual layout tree for a document

Also Published As

Publication number Publication date
ES2819028T3 (es) 2021-04-14
TWI740180B (zh) 2021-09-21
TW201831678A (zh) 2018-09-01
TWI686478B (zh) 2020-03-01
AU2016210783B2 (en) 2018-06-28
NZ709124A (en) 2017-06-30
AR122249A2 (es) 2022-08-31
CL2012001117A1 (es) 2013-03-22
RU2012121428A (ru) 2013-12-10
IL251763A0 (en) 2017-06-29
KR102016870B1 (ko) 2019-08-30
TW202022111A (zh) 2020-06-16
CA2779144A1 (en) 2011-05-05
MX2012005087A (es) 2012-09-28
JP6356165B2 (ja) 2018-07-11
TWI641688B (zh) 2018-11-21
RU2016104823A (ru) 2018-11-22
CN105456292A (zh) 2016-04-06
IL219454A0 (en) 2012-06-28
EP2494033B1 (en) 2020-07-01
JP5936547B2 (ja) 2016-06-22
AU2016210783A1 (en) 2016-09-01
EP2494033A1 (en) 2012-09-05
RU2580246C2 (ru) 2016-04-10
JP2016127847A (ja) 2016-07-14
TW201122108A (en) 2011-07-01
BR112012009848B1 (pt) 2021-11-03
CA3169831C (en) 2025-11-18
AR078849A1 (es) 2011-12-07
CA2779144C (en) 2022-10-25
CR20120254A (es) 2012-09-24
WO2011053690A1 (en) 2011-05-05
JP2013509187A (ja) 2013-03-14
PH12012500845A1 (en) 2013-01-07
KR20180055931A (ko) 2018-05-25
EP2494033A4 (en) 2015-07-29
CN102918145B (zh) 2016-01-27
CN105456292B (zh) 2019-03-26
BR112012009848A2 (pt) 2015-09-15
US20110135610A1 (en) 2011-06-09
RU2756584C2 (ru) 2021-10-01
NZ724435A (en) 2021-02-26
SG10201913006XA (en) 2020-02-27
EP3725874A1 (en) 2020-10-21
SG10201407102QA (en) 2014-11-27
KR20120117758A (ko) 2012-10-24
IL219454A (en) 2017-05-29
KR20190065476A (ko) 2019-06-11
NZ622427A (en) 2015-10-30
RU2016104823A3 (cg-RX-API-DMAC7.html) 2019-08-19
CA3169831A1 (en) 2011-05-05
NZ600068A (en) 2014-05-30
CN102918145A (zh) 2013-02-06
AU2010313386A1 (en) 2012-06-14

Similar Documents

Publication Publication Date Title
KR102016870B1 (ko) 간외 담도계로부터의 다분화능 줄기 세포 및 이의 분리 방법
KR101195838B1 (ko) 분리된 전분화능 성체줄기세포 및 그의 분리 및 배양 방법
Semeraro et al. Multipotent stem/progenitor cells in the human foetal biliary tree
CN109136163A (zh) 用于干细胞的培养基
JP2005521405A (ja) 単球を起源に持つ、脱分化したプログラム可能な幹細胞およびそれらの製造と使用
AU2010313386B2 (en) Multipotent stem cells from the extrahepatic billary tree and methods of isolating same
RU2663118C1 (ru) Клеточный продукт инсулин-продуцирующих клеток млекопитающих и его использование для терапии сахарного диабета
NZ724435B2 (en) Multipotent stem cells from the extrahepatic biliary tree and methods of isolating same
TW201741451A (zh) 一種用於生物工程化組織的組合物與方法

Legal Events

Date Code Title Description
A107 Divisional application of patent
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20201221

Application number text: 1020197015956

Filing date: 20190603

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20210118

Comment text: Request for Examination of Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20210330

Patent event code: PE09021S01D

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20220228

Patent event code: PE09021S01D

E601 Decision to refuse application
PE0601 Decision on rejection of patent

Patent event date: 20230130

Comment text: Decision to Refuse Application

Patent event code: PE06012S01D

Patent event date: 20220228

Comment text: Notification of reason for refusal

Patent event code: PE06011S01I

Patent event date: 20210330

Comment text: Notification of reason for refusal

Patent event code: PE06011S01I