KR20150132479A - 복합 유전자 세트에서의 독특하게 태그되고 재배열된 적응 면역 수용체 유전자 - Google Patents

복합 유전자 세트에서의 독특하게 태그되고 재배열된 적응 면역 수용체 유전자 Download PDF

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KR20150132479A
KR20150132479A KR1020157029634A KR20157029634A KR20150132479A KR 20150132479 A KR20150132479 A KR 20150132479A KR 1020157029634 A KR1020157029634 A KR 1020157029634A KR 20157029634 A KR20157029634 A KR 20157029634A KR 20150132479 A KR20150132479 A KR 20150132479A
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immunoreceptor
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oligonucleotide
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할란 에스. 로빈스
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어댑티브 바이오테크놀로지스 코포레이션
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KR1020157029634A 2013-03-15 2014-03-17 복합 유전자 세트에서의 독특하게 태그되고 재배열된 적응 면역 수용체 유전자 Withdrawn KR20150132479A (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361789408P 2013-03-15 2013-03-15
US61/789,408 2013-03-15
PCT/US2013/045994 WO2013188831A1 (en) 2012-06-15 2013-06-14 Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
USPCT/US2013/045994 2013-06-14
PCT/US2014/030859 WO2014145992A1 (en) 2013-03-15 2014-03-17 Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set

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US (2) US20160024493A1 (enExample)
EP (1) EP2971105B1 (enExample)
JP (1) JP6431895B2 (enExample)
KR (1) KR20150132479A (enExample)
CN (1) CN105452483B (enExample)
AU (2) AU2014232314B2 (enExample)
CA (1) CA2906218A1 (enExample)
IL (1) IL241394B (enExample)
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WO (1) WO2014145992A1 (enExample)

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
DK3059337T3 (da) 2009-01-15 2019-07-22 Adaptive Biotechnologies Corp Adaptive immunity profiling og metoder til frembringelse af monoklonale antibodier
DK2823060T3 (en) 2012-03-05 2018-05-28 Adaptive Biotechnologies Corp Determination of associated immune receptor chains from frequency-matched subunits
EP3091089B1 (en) 2012-05-08 2019-01-23 Adaptive Biotechnologies Corporation Method for measuring and calibrating amplification bias in multiplexed pcr reactions
US10150996B2 (en) 2012-10-19 2018-12-11 Adaptive Biotechnologies Corp. Quantification of adaptive immune cell genomes in a complex mixture of cells
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
WO2015134787A2 (en) 2014-03-05 2015-09-11 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
US10202640B2 (en) * 2014-05-07 2019-02-12 The Board Of Trustees Of The Leland Stanford Junior University Single cell analysis of T cells using high-throughput multiplex amplification and deep sequencing
WO2015176162A1 (en) 2014-05-22 2015-11-26 The University Of British Columbia Methods for determining lymphocyte receptor chain pairs
CA2966201A1 (en) 2014-10-29 2016-05-06 Adaptive Biotechnologies Corp. Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
EP3224384A4 (en) 2014-11-25 2018-04-18 Adaptive Biotechnologies Corp. Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
CN104562215B (zh) * 2014-12-29 2017-03-22 北京诺禾致源科技股份有限公司 扩增子文库的构建方法
EP3247804B1 (en) * 2015-01-23 2020-08-05 Qiagen Sciences, LLC High multiplex pcr with molecular barcoding
US11047008B2 (en) 2015-02-24 2021-06-29 Adaptive Biotechnologies Corporation Methods for diagnosing infectious disease and determining HLA status using immune repertoire sequencing
WO2016138488A2 (en) * 2015-02-26 2016-09-01 The Broad Institute Inc. T cell balance gene expression, compositions of matters and methods of use thereof
WO2016161273A1 (en) 2015-04-01 2016-10-06 Adaptive Biotechnologies Corp. Method of identifying human compatible t cell receptors specific for an antigenic target
HK1252248A1 (zh) * 2015-04-27 2019-05-24 Abvitro Llc 对治疗剂和疾病特异性抗原进行测序、确定、配对和验证的方法
WO2017013170A1 (en) 2015-07-22 2017-01-26 F. Hoffmann-La Roche Ag Identification of antigen epitopes and immune sequences recognizing the antigens
US10539564B2 (en) 2015-07-22 2020-01-21 Roche Sequencing Solutions, Inc. Identification of antigen epitopes and immune sequences recognizing the antigens
GB201516269D0 (en) * 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516276D0 (en) * 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516265D0 (en) * 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516275D0 (en) * 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516277D0 (en) 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR libraries
GB201516274D0 (en) * 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516272D0 (en) 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516270D0 (en) * 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
AU2016348439B2 (en) * 2015-11-04 2023-03-09 Atreca, Inc. Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
EP4289965A3 (en) * 2016-02-12 2024-02-21 CureVac SE Method for analyzing rna
EP3423598B1 (en) * 2016-03-01 2024-05-01 Universal Sequencing Technology Corporation Methods and kits for tracking nucleic acid target origin for nucleic acid sequencing
US11920174B2 (en) 2016-03-03 2024-03-05 CureVac SE RNA analysis by total hydrolysis and quantification of released nucleosides
EP3452614B1 (en) 2016-05-02 2023-06-28 Becton, Dickinson and Company Accurate molecular barcoding
ES2898088T3 (es) 2016-06-01 2022-03-03 Hoffmann La Roche Inmuno-PETE
CN106283201B (zh) * 2016-09-20 2019-08-06 中国医学科学院肿瘤医院 基于高通量测序的tcr多样性检测和文库构建
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
RU2019123063A (ru) 2016-12-23 2021-01-26 Вистерра, Инк. Связывающие полипептиды и способы их получения
WO2018132635A1 (en) * 2017-01-12 2018-07-19 Massachusetts Institute Of Technology Methods for analyzing t cell receptors and b cell receptors
US10920273B2 (en) 2017-01-17 2021-02-16 Life Technologies Corporation Compositions and methods for immune repertoire sequencing
WO2018144410A1 (en) * 2017-01-31 2018-08-09 Ludwig Institute For Cancer Research Ltd. Enhanced immune cell receptor sequencing methods
WO2019046817A1 (en) 2017-09-01 2019-03-07 Life Technologies Corporation COMPOSITIONS AND METHODS FOR IMMUNOLOGICAL REPERTOIRE SEQUENCING
CN109536594B (zh) * 2017-09-20 2022-11-01 深圳华大智造科技股份有限公司 一种用于Small RNA的测序方法、测序试剂和应用
WO2019084552A1 (en) 2017-10-27 2019-05-02 The Regents Of The University Of California TARGETED REPLACEMENT OF ENDOGENIC T CELL RECEPTORS
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
CN108374203B (zh) * 2018-03-01 2021-07-23 艾吉泰康生物科技(北京)有限公司 一种用于双向测序的扩增子文库的构建方法
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
EP3824103A1 (en) 2018-07-18 2021-05-26 Life Technologies Corporation Compositions and methods for immune repertoire sequencing
US20220002802A1 (en) * 2018-07-18 2022-01-06 Life Technologies Corporation Compositions and methods for immune repertoire sequencing
EP3841219A4 (en) 2018-08-24 2022-05-04 Swift Biosciences, Inc. ASYMMETRIC TARGETED AMPLIFICATION METHODS
EP3966345A4 (en) * 2019-05-10 2023-06-28 The Chinese University of Hong Kong Primers and assays for linking regions using polymerases
EP3980559A1 (en) * 2019-06-06 2022-04-13 Natera, Inc. Methods for detecting immune cell dna and monitoring immune system
US11149322B2 (en) * 2019-06-07 2021-10-19 Chapter Diagnostics, Inc. Methods and compositions for human papillomaviruses and sexually transmitted infections detection, identification and quantification
US20220308061A1 (en) * 2019-09-03 2022-09-29 The Regents Of The University Of California Method to sequence mrna in single cells in parallel with quantification of intracellular phenotype
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
EP4139486A4 (en) * 2020-04-24 2024-10-16 The Board of Trustees of the Leland Stanford Junior University Novel t-cell specificities and uses thereof
US10941453B1 (en) * 2020-05-20 2021-03-09 Paragon Genomics, Inc. High throughput detection of pathogen RNA in clinical specimens
US20240011020A1 (en) * 2020-09-08 2024-01-11 Seqwell, Inc Sequencing oligonucleotides and methods of use thereof
US11111489B1 (en) 2021-01-06 2021-09-07 Think Therapeutics, Inc. Multiplexed testing of lymphocytes for antigen specificity
CA3214486A1 (en) 2021-03-24 2022-09-29 Genentech, Inc. Efficient tcr gene editing in t lymphocytes
EP4314337A1 (en) * 2021-04-01 2024-02-07 F. Hoffmann-La Roche AG Immune cell counting of sars-cov-2 patients based on immune repertoire sequencing
CN114561454B (zh) * 2022-03-03 2025-11-14 中国人民解放军陆军军医大学 一种基于高通量测序对TCR β进行定量的方法
US12091715B2 (en) 2022-04-21 2024-09-17 Paragon Genomics, Inc. Methods and compositions for reducing base errors of massive parallel sequencing using triseq sequencing
US11680293B1 (en) 2022-04-21 2023-06-20 Paragon Genomics, Inc. Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules
TWI867406B (zh) * 2022-10-27 2024-12-21 醫華生技股份有限公司 生物微粒分析方法
EP4603595A1 (en) * 2024-02-13 2025-08-20 ImmuneDiscover Sweden AB A method for typing the immune genes and the allelic variants thereof

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US6031091A (en) 1987-09-21 2000-02-29 Gen-Probe Incorporated Non-nucleotide linking reagents for nucleotide probes
KR920003787B1 (ko) 1990-03-05 1992-05-14 한국과학기술 연구원 신규 플라스미드 제조 및 그를 함유한 균주를 배양하여 아이 지 에프-1(igf-1)을 생산하는 방법
CA2135073C (en) 1992-05-06 2002-11-19 Daniel L. Kacian Nucleic acid sequence amplification method, composition and kit
US5596090A (en) 1992-07-24 1997-01-21 The United States Of America As Represented By The Secretary Of The Navy Antisense oligonucleotides directed against human VCAM-1 RNA
US5571711A (en) 1993-06-17 1996-11-05 Ludwig Institute For Cancer Research Isolated nucleic acid molecules coding for BAGE tumor rejection antigen precursors
DE69533295T3 (de) 1994-02-16 2009-07-16 The Government Of The United States Of America, As Represented By The Secretary, The Department Of Health And Human Services Melanoma-assoziierte Antigene, Epitope davon und Impstoffe gegen Melanoma
US5821122A (en) 1995-06-07 1998-10-13 Inserm (Institute Nat'l De La Sante Et De La Recherche . .) Isolated nucleic acid molecules, peptides which form complexes with MHC molecule HLA-A2 and uses thereof
JPH0975090A (ja) 1995-09-13 1997-03-25 Kanegafuchi Chem Ind Co Ltd 細胞付着部材
AU2006259565B2 (en) * 2005-06-15 2011-01-06 Complete Genomics, Inc. Single molecule arrays for genetic and chemical analysis
US8911948B2 (en) 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP2364368B1 (en) * 2008-11-07 2014-01-15 Sequenta, Inc. Methods of monitoring conditions by sequence analysis
RU2014144463A (ru) 2009-06-25 2015-06-20 Фред Хатчинсон Кансэр Рисёч Сентер Способ измерения адаптивного иммунитета
US20120058902A1 (en) 2009-06-25 2012-03-08 Livingston Robert J Method of measuring adaptive immunity
WO2011106738A2 (en) 2010-02-25 2011-09-01 Fred Hutchinson Cancer Research Center Use of tcr clonotypes as biomarkers for disease
WO2011139372A1 (en) * 2010-05-06 2011-11-10 Sequenta, Inc. Sequence analysis of complex amplicons
CN103069006A (zh) * 2010-07-23 2013-04-24 艾索特里克斯遗传实验室有限责任公司 区别表达的胚胎或母源基因组区的鉴定及其用途
US9193997B2 (en) * 2010-12-15 2015-11-24 The Board Of Trustees Of The Leland Stanford Junior University Measuring and monitoring of cell clonality
ES2615733T3 (es) 2010-12-16 2017-06-08 Gigagen, Inc. Métodos para el análisis masivo paralelo de ácidos nucleicos en células individuales
CN108220297A (zh) * 2011-03-09 2018-06-29 细胞信号科技公司 用于生成单克隆抗体的方法和试剂
PL3892295T3 (pl) * 2011-05-24 2023-07-24 BioNTech SE Zindywidualizowane szczepionki przeciwnowotworowe
DK2823060T3 (en) * 2012-03-05 2018-05-28 Adaptive Biotechnologies Corp Determination of associated immune receptor chains from frequency-matched subunits

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JP2016512685A (ja) 2016-05-09
IL241394A0 (en) 2015-11-30
EP2971105B1 (en) 2018-08-01
SG11201506991VA (en) 2015-10-29
CN105452483B (zh) 2019-01-11
AU2020213348A1 (en) 2020-08-27
AU2014232314A1 (en) 2015-09-17
AU2014232314B2 (en) 2020-05-14
AU2020213348B2 (en) 2022-05-19
WO2014145992A1 (en) 2014-09-18
CA2906218A1 (en) 2014-09-18
SG10201707394PA (en) 2017-10-30
CN105452483A (zh) 2016-03-30
US20160024493A1 (en) 2016-01-28
EP2971105A1 (en) 2016-01-20
US20250043272A1 (en) 2025-02-06
JP6431895B2 (ja) 2018-12-05

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