JP6431895B2 - 複合遺伝子セットにおいてユニークにタグ付けされた再構成された適応免疫受容体遺伝子 - Google Patents

複合遺伝子セットにおいてユニークにタグ付けされた再構成された適応免疫受容体遺伝子 Download PDF

Info

Publication number
JP6431895B2
JP6431895B2 JP2016502574A JP2016502574A JP6431895B2 JP 6431895 B2 JP6431895 B2 JP 6431895B2 JP 2016502574 A JP2016502574 A JP 2016502574A JP 2016502574 A JP2016502574 A JP 2016502574A JP 6431895 B2 JP6431895 B2 JP 6431895B2
Authority
JP
Japan
Prior art keywords
sequence
sequences
oligonucleotide
air
primer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2016502574A
Other languages
English (en)
Japanese (ja)
Other versions
JP2016512685A (ja
JP2016512685A5 (enExample
Inventor
ロビンズ，ハーラン，エス．
Original Assignee
アダプティブ バイオテクノロジーズ コーポレイション
アダプティブ バイオテクノロジーズ コーポレイション
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2013/045994 external-priority patent/WO2013188831A1/en
Application filed by アダプティブ バイオテクノロジーズ コーポレイション, アダプティブ バイオテクノロジーズ コーポレイション filed Critical アダプティブ バイオテクノロジーズ コーポレイション
Publication of JP2016512685A publication Critical patent/JP2016512685A/ja
Publication of JP2016512685A5 publication Critical patent/JP2016512685A5/ja
Application granted granted Critical
Publication of JP6431895B2 publication Critical patent/JP6431895B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2016502574A 2013-03-15 2014-03-17 複合遺伝子セットにおいてユニークにタグ付けされた再構成された適応免疫受容体遺伝子 Active JP6431895B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361789408P 2013-03-15 2013-03-15
US61/789,408 2013-03-15
USPCT/US2013/045994 2013-06-14
PCT/US2013/045994 WO2013188831A1 (en) 2012-06-15 2013-06-14 Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
PCT/US2014/030859 WO2014145992A1 (en) 2013-03-15 2014-03-17 Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set

Publications (3)

Publication Number Publication Date
JP2016512685A JP2016512685A (ja) 2016-05-09
JP2016512685A5 JP2016512685A5 (enExample) 2017-04-13
JP6431895B2 true JP6431895B2 (ja) 2018-12-05

Family

ID=51538155

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2016502574A Active JP6431895B2 (ja) 2013-03-15 2014-03-17 複合遺伝子セットにおいてユニークにタグ付けされた再構成された適応免疫受容体遺伝子

Country Status (10)

Country Link
US (2) US20160024493A1 (enExample)
EP (1) EP2971105B1 (enExample)
JP (1) JP6431895B2 (enExample)
KR (1) KR20150132479A (enExample)
CN (1) CN105452483B (enExample)
AU (2) AU2014232314B2 (enExample)
CA (1) CA2906218A1 (enExample)
IL (1) IL241394B (enExample)
SG (2) SG10201707394PA (enExample)
WO (1) WO2014145992A1 (enExample)

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US8685898B2 (en) 2009-01-15 2014-04-01 Imdaptive, Inc. Adaptive immunity profiling and methods for generation of monoclonal antibodies
US10077478B2 (en) 2012-03-05 2018-09-18 Adaptive Biotechnologies Corp. Determining paired immune receptor chains from frequency matched subunits
MX354329B (es) 2012-05-08 2018-02-27 Adaptive Biotechnologies Corp Composiciones y metodos para medir y calibrar el error de amplificacion en reacciones de pcr multiple.
WO2015160439A2 (en) 2014-04-17 2015-10-22 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
WO2015134787A2 (en) 2014-03-05 2015-09-11 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
US10202640B2 (en) * 2014-05-07 2019-02-12 The Board Of Trustees Of The Leland Stanford Junior University Single cell analysis of T cells using high-throughput multiplex amplification and deep sequencing
US10400281B2 (en) 2014-05-22 2019-09-03 The University Of British Columbia Methods for determining lymphocyte receptor chain pairs
ES2784343T3 (es) 2014-10-29 2020-09-24 Adaptive Biotechnologies Corp Detección simultánea altamente multiplexada de ácidos nucleicos que codifican heterodímeros de receptores inmunes adaptativos emparejados de muchas muestras
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
AU2015353581A1 (en) 2014-11-25 2017-06-15 Adaptive Biotechnologies Corporation Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
CN104562215B (zh) * 2014-12-29 2017-03-22 北京诺禾致源科技股份有限公司 扩增子文库的构建方法
US10760120B2 (en) * 2015-01-23 2020-09-01 Qiagen Sciences, Llc High multiplex PCR with molecular barcoding
US11047008B2 (en) 2015-02-24 2021-06-29 Adaptive Biotechnologies Corporation Methods for diagnosing infectious disease and determining HLA status using immune repertoire sequencing
EP3262193A2 (en) * 2015-02-26 2018-01-03 The Broad Institute Inc. T cell balance gene expression, compositions of matters and methods of use thereof
EP3277294B1 (en) 2015-04-01 2024-05-15 Adaptive Biotechnologies Corp. Method of identifying human compatible t cell receptors specific for an antigenic target
CN107849560B (zh) * 2015-04-27 2021-10-22 阿布维特罗有限责任公司 对治疗剂和疾病特异性抗原进行测序、确定、配对和验证的方法
WO2017013170A1 (en) 2015-07-22 2017-01-26 F. Hoffmann-La Roche Ag Identification of antigen epitopes and immune sequences recognizing the antigens
US10539564B2 (en) 2015-07-22 2020-01-21 Roche Sequencing Solutions, Inc. Identification of antigen epitopes and immune sequences recognizing the antigens
GB201516276D0 (en) * 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516274D0 (en) * 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516277D0 (en) 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR libraries
GB201516272D0 (en) 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516265D0 (en) * 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516275D0 (en) * 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516269D0 (en) * 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
GB201516270D0 (en) * 2015-09-15 2015-10-28 Adaptimmune Ltd And Immunocore Ltd TCR Libraries
WO2017079593A1 (en) * 2015-11-04 2017-05-11 Atreca, Inc. Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
EP3414340B1 (en) * 2016-02-12 2023-08-09 CureVac SE Method for analyzing rna
US20190078150A1 (en) * 2016-03-01 2019-03-14 Universal Sequencing Technology Corporation Methods and Kits for Tracking Nucleic Acid Target Origin for Nucleic Acid Sequencing
EP3423595A1 (en) 2016-03-03 2019-01-09 CureVac AG Rna analysis by total hydrolysis
WO2017192387A1 (en) 2016-05-02 2017-11-09 Cellular Research, Inc. Accurate molecular barcoding
WO2017210469A2 (en) * 2016-06-01 2017-12-07 F. Hoffman-La Roche Ag Immuno-pete
CN106283201B (zh) * 2016-09-20 2019-08-06 中国医学科学院肿瘤医院 基于高通量测序的tcr多样性检测和文库构建
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
JP7360324B2 (ja) 2016-12-23 2023-10-12 ビステラ， インコーポレイテッド 結合ポリペプチドおよびそれを作製する方法
US20210087610A9 (en) * 2017-01-12 2021-03-25 Massachusetts Institute Of Technology Methods for analyzing t cell receptors and b cell receptors
CN110249060A (zh) * 2017-01-17 2019-09-17 生命技术公司 用于免疫组库测序的组合物和方法
US11319590B2 (en) * 2017-01-31 2022-05-03 Ludwig Institute For Cancer Research Ltd. Enhanced immune cell receptor sequencing methods
EP3676399B1 (en) 2017-09-01 2022-05-11 Life Technologies Corporation Compositions and methods for immune repertoire sequencing
CN109536594B (zh) * 2017-09-20 2022-11-01 深圳华大智造科技股份有限公司 一种用于Small RNA的测序方法、测序试剂和应用
IL305485B2 (en) * 2017-10-27 2025-10-01 Univ California Targeted replacement of endogenous T cell receptors
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
CN108374203B (zh) * 2018-03-01 2021-07-23 艾吉泰康生物科技(北京)有限公司 一种用于双向测序的扩增子文库的构建方法
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
WO2020018836A1 (en) 2018-07-18 2020-01-23 Life Technologies Corporation Compositions and methods for immune repertoire sequencing
WO2020018837A1 (en) * 2018-07-18 2020-01-23 Life Technologies Corporation Compositions and methods for immune repertoire sequencing
WO2020041702A1 (en) 2018-08-24 2020-02-27 Swift Biosciences, Inc. Asymmetric targeted amplification methods
CA3139178A1 (en) * 2019-05-10 2020-11-19 The Chinese University Of Hong Kong Primers and assays for linking regions using polymerases
WO2020247263A1 (en) * 2019-06-06 2020-12-10 Natera, Inc. Methods for detecting immune cell dna and monitoring immune system
US11149322B2 (en) * 2019-06-07 2021-10-19 Chapter Diagnostics, Inc. Methods and compositions for human papillomaviruses and sexually transmitted infections detection, identification and quantification
WO2021046121A1 (en) * 2019-09-03 2021-03-11 The Regents Of The University Of California Method to sequence mrna in single cells in parallel with quantification of intracellular phenotype
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
JP2023515265A (ja) * 2020-04-24 2023-04-12 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 新規ｔ細胞特異性とその使用
US10941453B1 (en) * 2020-05-20 2021-03-09 Paragon Genomics, Inc. High throughput detection of pathogen RNA in clinical specimens
EP4211239A4 (en) * 2020-09-08 2024-09-11 Seqwell, Inc. Sequencing oligonucleotides and methods of use thereof
US11111489B1 (en) 2021-01-06 2021-09-07 Think Therapeutics, Inc. Multiplexed testing of lymphocytes for antigen specificity
KR20230160885A (ko) 2021-03-24 2023-11-24 제넨테크, 인크. T 림프구에서 효율적인 tcr 유전자 편집
WO2022207682A1 (en) * 2021-04-01 2022-10-06 F. Hoffmann-La Roche Ag Immune cell counting of sars-cov-2 patients based on immune repertoire sequencing
CN114561454B (zh) * 2022-03-03 2025-11-14 中国人民解放军陆军军医大学 一种基于高通量测序对TCR β进行定量的方法
US11680293B1 (en) 2022-04-21 2023-06-20 Paragon Genomics, Inc. Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules
US12091715B2 (en) 2022-04-21 2024-09-17 Paragon Genomics, Inc. Methods and compositions for reducing base errors of massive parallel sequencing using triseq sequencing
TWI867406B (zh) * 2022-10-27 2024-12-21 醫華生技股份有限公司 生物微粒分析方法
EP4603595A1 (en) * 2024-02-13 2025-08-20 ImmuneDiscover Sweden AB A method for typing the immune genes and the allelic variants thereof

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US6031091A (en) 1987-09-21 2000-02-29 Gen-Probe Incorporated Non-nucleotide linking reagents for nucleotide probes
KR920003787B1 (ko) 1990-03-05 1992-05-14 한국과학기술 연구원 신규 플라스미드 제조 및 그를 함유한 균주를 배양하여 아이 지 에프-1(igf-1)을 생산하는 방법
CA2135073C (en) 1992-05-06 2002-11-19 Daniel L. Kacian Nucleic acid sequence amplification method, composition and kit
US5596090A (en) 1992-07-24 1997-01-21 The United States Of America As Represented By The Secretary Of The Navy Antisense oligonucleotides directed against human VCAM-1 RNA
US5571711A (en) 1993-06-17 1996-11-05 Ludwig Institute For Cancer Research Isolated nucleic acid molecules coding for BAGE tumor rejection antigen precursors
ATE272113T1 (de) 1994-02-16 2004-08-15 Crucell Holland Bv Melanoma-assoziierte antigene, epitope davon und impstoffe gegen melanoma
US5821122A (en) 1995-06-07 1998-10-13 Inserm (Institute Nat'l De La Sante Et De La Recherche . .) Isolated nucleic acid molecules, peptides which form complexes with MHC molecule HLA-A2 and uses thereof
JPH0975090A (ja) 1995-09-13 1997-03-25 Kanegafuchi Chem Ind Co Ltd 細胞付着部材
DK1907571T3 (en) * 2005-06-15 2017-08-21 Complete Genomics Inc NUCLEIC ACID ANALYSIS USING INCIDENTAL MIXTURES OF NON-OVERLAPPING FRAGMENTS
US8911948B2 (en) 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US8236503B2 (en) * 2008-11-07 2012-08-07 Sequenta, Inc. Methods of monitoring conditions by sequence analysis
CA2765949C (en) 2009-06-25 2016-03-29 Fred Hutchinson Cancer Research Center Method of measuring adaptive immunity
US20120058902A1 (en) 2009-06-25 2012-03-08 Livingston Robert J Method of measuring adaptive immunity
WO2011106738A2 (en) 2010-02-25 2011-09-01 Fred Hutchinson Cancer Research Center Use of tcr clonotypes as biomarkers for disease
ES2593614T3 (es) * 2010-05-06 2016-12-12 Adaptive Biotechnologies Corporation Monitorización de estados de salud y enfermedad usando perfiles de clonotipos
US20120021919A1 (en) * 2010-07-23 2012-01-26 Thomas Scholl Identification of Differentially Represented Fetal or Maternal Genomic Regions and Uses Thereof
WO2012083069A2 (en) * 2010-12-15 2012-06-21 The Board Of Trustees Of The Leland Stanford Junior University Measurement and monitoring of cell clonality
DK2652155T3 (en) 2010-12-16 2017-02-13 Gigagen Inc Methods for Massive Parallel Analysis of Nucleic Acids in Single Cells
EP3345922A1 (en) * 2011-03-09 2018-07-11 Cell Signaling Technology, Inc. Methods and reagents for creating monoclonal antibodies
HRP20230443T1 (hr) * 2011-05-24 2023-09-15 BioNTech SE Individualizirana cjepiva protiv raka
US10077478B2 (en) * 2012-03-05 2018-09-18 Adaptive Biotechnologies Corp. Determining paired immune receptor chains from frequency matched subunits

Also Published As

Publication number Publication date
US20160024493A1 (en) 2016-01-28
KR20150132479A (ko) 2015-11-25
US20250043272A1 (en) 2025-02-06
WO2014145992A1 (en) 2014-09-18
JP2016512685A (ja) 2016-05-09
AU2014232314A1 (en) 2015-09-17
AU2020213348A1 (en) 2020-08-27
CA2906218A1 (en) 2014-09-18
SG10201707394PA (en) 2017-10-30
SG11201506991VA (en) 2015-10-29
AU2020213348B2 (en) 2022-05-19
IL241394B (en) 2019-12-31
EP2971105A1 (en) 2016-01-20
EP2971105B1 (en) 2018-08-01
IL241394A0 (en) 2015-11-30
AU2014232314B2 (en) 2020-05-14
CN105452483B (zh) 2019-01-11
CN105452483A (zh) 2016-03-30

Similar Documents

Publication Publication Date Title
JP6431895B2 (ja) 複合遺伝子セットにおいてユニークにタグ付けされた再構成された適応免疫受容体遺伝子
AU2013273987B2 (en) Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
US11591652B2 (en) System and methods for massively parallel analysis of nucleic acids in single cells
US11427933B2 (en) Systems and methods for massively parallel combinatorial analysis of single cells
US20150154352A1 (en) System and Methods for Genetic Analysis of Mixed Cell Populations
US20250066852A1 (en) Kit and Method for Analyzing T Cell Receptors from Single T Cells

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170224

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170310

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20170310

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20180213

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180514

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20181023

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20181105

R150 Certificate of patent or registration of utility model

Ref document number: 6431895

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250