KR20150076172A - 이질-이합체 면역글로불린의 정제 - Google Patents

이질-이합체 면역글로불린의 정제 Download PDF

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Publication number
KR20150076172A
KR20150076172A KR1020157010732A KR20157010732A KR20150076172A KR 20150076172 A KR20150076172 A KR 20150076172A KR 1020157010732 A KR1020157010732 A KR 1020157010732A KR 20157010732 A KR20157010732 A KR 20157010732A KR 20150076172 A KR20150076172 A KR 20150076172A
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South Korea
Prior art keywords
immunoglobulin
fragment
protein
constant region
amino acid
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Ceased
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KR1020157010732A
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English (en)
Korean (ko)
Inventor
스타니슬라스 블랭
파브리지오 컴퍼
로메인 올리에
폴 와스맨
Original Assignee
그렌마크 파머수티칼스 에스. 아.
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Publication of KR20150076172A publication Critical patent/KR20150076172A/ko
Ceased legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46Hybrid immunoglobulins
    • C07K16/468Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804Affinity chromatography
    • B01D15/3809Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/22Affinity chromatography or related techniques based upon selective absorption processes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/30Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52Constant or Fc region; Isotype
    • C07K2317/522CH1 domain
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52Constant or Fc region; Isotype
    • C07K2317/524CH2 domain
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52Constant or Fc region; Isotype
    • C07K2317/526CH3 domain
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565Complementarity determining region [CDR]
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/567Framework region [FR]
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/60Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622Single chain antibody (scFv)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/90Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
KR1020157010732A 2012-09-25 2013-09-25 이질-이합체 면역글로불린의 정제 Ceased KR20150076172A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261705278P 2012-09-25 2012-09-25
US61/705,278 2012-09-25
PCT/EP2013/069989 WO2014049003A1 (en) 2012-09-25 2013-09-25 Purification of hetero-dimeric immunoglobulins

Publications (1)

Publication Number Publication Date
KR20150076172A true KR20150076172A (ko) 2015-07-06

Family

ID=49237223

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020157010732A Ceased KR20150076172A (ko) 2012-09-25 2013-09-25 이질-이합체 면역글로불린의 정제

Country Status (11)

Country Link
US (2) US20150239991A1 (enExample)
EP (2) EP2900696A1 (enExample)
JP (5) JP2015529236A (enExample)
KR (1) KR20150076172A (enExample)
CN (1) CN104968685A (enExample)
AU (2) AU2013322710A1 (enExample)
CA (2) CA3061557A1 (enExample)
HK (1) HK1215950A1 (enExample)
IL (2) IL237872B (enExample)
IN (1) IN2015MN00139A (enExample)
WO (1) WO2014049003A1 (enExample)

Cited By (1)

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Publication number Priority date Publication date Assignee Title
WO2021130717A1 (ko) * 2019-12-26 2021-07-01 에이비엘바이오 주식회사 단백질 a 친화성 크로마토그래피를 이용한 생리활성 펩티드의 정제 방법

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Publication number Priority date Publication date Assignee Title
WO2021130717A1 (ko) * 2019-12-26 2021-07-01 에이비엘바이오 주식회사 단백질 a 친화성 크로마토그래피를 이용한 생리활성 펩티드의 정제 방법

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JP2015529236A (ja) 2015-10-05
CA3061557A1 (en) 2014-04-03
HK1215950A1 (zh) 2016-09-30
EP3401337A1 (en) 2018-11-14
CA2886036A1 (en) 2014-04-03
US20150239991A1 (en) 2015-08-27
US20200010568A1 (en) 2020-01-09
AU2013322710A1 (en) 2015-04-16
IL237872B (en) 2019-09-26
JP6856610B2 (ja) 2021-04-07
JP2018104444A (ja) 2018-07-05
JP2020105203A (ja) 2020-07-09
JP2019073512A (ja) 2019-05-16
JP2022116326A (ja) 2022-08-09
AU2018204314A1 (en) 2018-07-05
EP2900696A1 (en) 2015-08-05
AU2013322710A2 (en) 2015-05-07
IN2015MN00139A (enExample) 2015-10-16
IL237872A0 (en) 2015-05-31
IL269207A (en) 2019-11-28
WO2014049003A1 (en) 2014-04-03
AU2018204314B2 (en) 2020-05-14
IL269207B (en) 2022-02-01
CN104968685A (zh) 2015-10-07

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