KR20130138760A - 고농도의 에이코사펜타엔산 생성을 위한 재조합 미생물 숙주 세포 - Google Patents

고농도의 에이코사펜타엔산 생성을 위한 재조합 미생물 숙주 세포 Download PDF

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KR20130138760A
KR20130138760A KR1020137007481A KR20137007481A KR20130138760A KR 20130138760 A KR20130138760 A KR 20130138760A KR 1020137007481 A KR1020137007481 A KR 1020137007481A KR 20137007481 A KR20137007481 A KR 20137007481A KR 20130138760 A KR20130138760 A KR 20130138760A
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seq
delta
epa
tfa
egd9es
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승-표 홍
파멜라 엘. 샤프
지시옹 수에
나렌드라 에스. 야다브
홍시앙 장
쿤 주
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이 아이 듀폰 디 네모아 앤드 캄파니
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    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • General Engineering & Computer Science (AREA)
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  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020137007481A 2010-08-26 2011-08-26 고농도의 에이코사펜타엔산 생성을 위한 재조합 미생물 숙주 세포 Withdrawn KR20130138760A (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US37724810P 2010-08-26 2010-08-26
US61/377,248 2010-08-26
US201061428277P 2010-12-30 2010-12-30
US61/428,277 2010-12-30
US201161479921P 2011-04-28 2011-04-28
US61/479,921 2011-04-28
PCT/US2011/049384 WO2012027689A1 (en) 2010-08-26 2011-08-26 Recombinant microbial host cells for high eicosapentaenoic acid production

Publications (1)

Publication Number Publication Date
KR20130138760A true KR20130138760A (ko) 2013-12-19

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KR1020137007481A Withdrawn KR20130138760A (ko) 2010-08-26 2011-08-26 고농도의 에이코사펜타엔산 생성을 위한 재조합 미생물 숙주 세포

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US (1) US8703473B2 (enExample)
EP (1) EP2609203B1 (enExample)
JP (1) JP2013535987A (enExample)
KR (1) KR20130138760A (enExample)
CN (1) CN103249834B (enExample)
AU (1) AU2011293180B2 (enExample)
BR (1) BR112013004351A2 (enExample)
CA (1) CA2807834C (enExample)
CL (1) CL2013000533A1 (enExample)
DK (1) DK2609203T3 (enExample)
WO (1) WO2012027689A1 (enExample)

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CN108330114B (zh) * 2018-03-21 2020-11-03 北京大学 一种利用epa的甘油二酯酰基转移酶及其应用
US11913046B2 (en) 2018-04-23 2024-02-27 Inbiose N.V. Increasing export of 2'fucosyllactose from microbial cells through the expression of a heterologous nucleic acid
WO2020082221A1 (zh) * 2018-10-23 2020-04-30 利时雨 重组磷酸胆碱胞苷转移酶的粘红酵母携带外源多肽的活细胞脂质体及其应用
CN116555054B (zh) * 2023-06-09 2024-05-14 陕西海斯夫生物工程有限公司 一株高产dha的重组裂殖壶菌、其构建方法及应用

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EP2609203A1 (en) 2013-07-03
BR112013004351A2 (pt) 2016-05-31
AU2011293180B2 (en) 2017-03-02
DK2609203T3 (da) 2018-10-01
AU2011293180A1 (en) 2013-01-24
JP2013535987A (ja) 2013-09-19
WO2012027689A1 (en) 2012-03-01
US20120052537A1 (en) 2012-03-01
US8703473B2 (en) 2014-04-22
CN103249834A (zh) 2013-08-14
CN103249834B (zh) 2016-10-26
EP2609203B1 (en) 2018-06-20
CA2807834C (en) 2019-06-25
CL2013000533A1 (es) 2013-10-18
CA2807834A1 (en) 2012-03-01

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