CA2807834C - Recombinant microbial host cells for high eicosapentaenoic acid production - Google Patents

Recombinant microbial host cells for high eicosapentaenoic acid production Download PDF

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Publication number
CA2807834C
CA2807834C CA2807834A CA2807834A CA2807834C CA 2807834 C CA2807834 C CA 2807834C CA 2807834 A CA2807834 A CA 2807834A CA 2807834 A CA2807834 A CA 2807834A CA 2807834 C CA2807834 C CA 2807834C
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Canada
Prior art keywords
seq
delta
elongase
epa
gene
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Expired - Fee Related
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CA2807834A
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English (en)
French (fr)
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CA2807834A1 (en
Inventor
Seung-Pyo Hong
Pamela L. Sharpe
Zhixiong Xue
Narendra S. Yadav
Hongxiang Zhang
Qun Zhu
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EIDP Inc
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EI Du Pont de Nemours and Co
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Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2807834A 2010-08-26 2011-08-26 Recombinant microbial host cells for high eicosapentaenoic acid production Expired - Fee Related CA2807834C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US37724810P 2010-08-26 2010-08-26
US61/377,248 2010-08-26
US201061428277P 2010-12-30 2010-12-30
US61/428,277 2010-12-30
US201161479921P 2011-04-28 2011-04-28
US61/479,921 2011-04-28
PCT/US2011/049384 WO2012027689A1 (en) 2010-08-26 2011-08-26 Recombinant microbial host cells for high eicosapentaenoic acid production

Publications (2)

Publication Number Publication Date
CA2807834A1 CA2807834A1 (en) 2012-03-01
CA2807834C true CA2807834C (en) 2019-06-25

Family

ID=45697764

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2807834A Expired - Fee Related CA2807834C (en) 2010-08-26 2011-08-26 Recombinant microbial host cells for high eicosapentaenoic acid production

Country Status (11)

Country Link
US (1) US8703473B2 (enExample)
EP (1) EP2609203B1 (enExample)
JP (1) JP2013535987A (enExample)
KR (1) KR20130138760A (enExample)
CN (1) CN103249834B (enExample)
AU (1) AU2011293180B2 (enExample)
BR (1) BR112013004351A2 (enExample)
CA (1) CA2807834C (enExample)
CL (1) CL2013000533A1 (enExample)
DK (1) DK2609203T3 (enExample)
WO (1) WO2012027689A1 (enExample)

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JP2013535988A (ja) * 2010-08-26 2013-09-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 変異ｈｐｇｇモチーフおよびｈｄａｓｈモチーフδ５デサチュラーゼおよび多価不飽和脂肪酸の製造におけるそれらの使用
US8969049B2 (en) * 2011-03-31 2015-03-03 E I Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
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JP2014516537A (ja) 2011-05-26 2014-07-17 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 組換え油性微生物における、その中のオイル含量を増加させるためのカレオシンの発現
WO2013192002A1 (en) 2012-06-19 2013-12-27 E. I. Du Pont De Nemours And Company IMPROVED PRODUCTION OF POLYUNSATURATED FATTY ACIDS BY COEXPRESSION OF ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES AND PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASES
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JP7011590B2 (ja) 2015-10-12 2022-02-10 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 細胞内での遺伝子組換えおよび相同組換えの増加のための保護ｄｎａ鋳型および使用方法
CN108330114B (zh) * 2018-03-21 2020-11-03 北京大学 一种利用epa的甘油二酯酰基转移酶及其应用
EP3784791A1 (en) 2018-04-23 2021-03-03 DuPont Nutrition Biosciences ApS Increasing export of 2' fucosyllactose from microbial cells through the expression of a heterologous nucleic acid
WO2020082221A1 (zh) * 2018-10-23 2020-04-30 利时雨 重组磷酸胆碱胞苷转移酶的粘红酵母携带外源多肽的活细胞脂质体及其应用
CN116555054B (zh) * 2023-06-09 2024-05-14 陕西海斯夫生物工程有限公司 一株高产dha的重组裂殖壶菌、其构建方法及应用

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Also Published As

Publication number Publication date
CL2013000533A1 (es) 2013-10-18
CN103249834B (zh) 2016-10-26
JP2013535987A (ja) 2013-09-19
US8703473B2 (en) 2014-04-22
AU2011293180A1 (en) 2013-01-24
BR112013004351A2 (pt) 2016-05-31
CA2807834A1 (en) 2012-03-01
AU2011293180B2 (en) 2017-03-02
EP2609203B1 (en) 2018-06-20
WO2012027689A1 (en) 2012-03-01
EP2609203A1 (en) 2013-07-03
CN103249834A (zh) 2013-08-14
US20120052537A1 (en) 2012-03-01
DK2609203T3 (da) 2018-10-01
KR20130138760A (ko) 2013-12-19

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