KR20130075787A - 식물에서 당단백질 생산의 변형 - Google Patents

식물에서 당단백질 생산의 변형 Download PDF

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Publication number
KR20130075787A
KR20130075787A KR1020137014893A KR20137014893A KR20130075787A KR 20130075787 A KR20130075787 A KR 20130075787A KR 1020137014893 A KR1020137014893 A KR 1020137014893A KR 20137014893 A KR20137014893 A KR 20137014893A KR 20130075787 A KR20130075787 A KR 20130075787A
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South Korea
Prior art keywords
protein
interest
plant
nucleotide sequence
galt
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Ceased
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KR1020137014893A
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Korean (ko)
Inventor
마르크-안드레 다우스트
에스텔르 마르케-블륀
뮤리엘 바르도
카롤 부렐
로익 파이
패트리스 르루즈
루이스-필리페 베지나
베로니크 고모
스테파니 아퀸
크리스토프 리후이
토마스 파칼레
크리스토프 소르일레
Original Assignee
메디카고 인코포레이티드
상뜨르 나시오날 드 라 리쉐르쉐 샹띠피끄
유니베르시떼 드 루앙
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Publication of KR20130075787A publication Critical patent/KR20130075787A/ko
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nutrition Science (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Cultivation Of Plants (AREA)
KR1020137014893A 2007-06-15 2008-06-13 식물에서 당단백질 생산의 변형 Ceased KR20130075787A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US94434407P 2007-06-15 2007-06-15
US60/944,344 2007-06-15
PCT/CA2008/001139 WO2008151440A1 (en) 2007-06-15 2008-06-13 Modifying glycoprotein production in plants

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020107000811A Division KR101385050B1 (ko) 2007-06-15 2008-06-13 식물에서 당단백질 생산의 변형

Publications (1)

Publication Number Publication Date
KR20130075787A true KR20130075787A (ko) 2013-07-05

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KR1020137014893A Ceased KR20130075787A (ko) 2007-06-15 2008-06-13 식물에서 당단백질 생산의 변형
KR1020107000811A Expired - Fee Related KR101385050B1 (ko) 2007-06-15 2008-06-13 식물에서 당단백질 생산의 변형

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KR1020107000811A Expired - Fee Related KR101385050B1 (ko) 2007-06-15 2008-06-13 식물에서 당단백질 생산의 변형

Country Status (24)

Country Link
US (1) US20110008837A1 (cg-RX-API-DMAC7.html)
EP (1) EP2155880B1 (cg-RX-API-DMAC7.html)
JP (1) JP5783720B2 (cg-RX-API-DMAC7.html)
KR (2) KR20130075787A (cg-RX-API-DMAC7.html)
CN (3) CN103382487A (cg-RX-API-DMAC7.html)
AU (1) AU2008261527C1 (cg-RX-API-DMAC7.html)
BR (1) BRPI0812538B1 (cg-RX-API-DMAC7.html)
CA (2) CA2700180C (cg-RX-API-DMAC7.html)
DK (1) DK2155880T3 (cg-RX-API-DMAC7.html)
EG (1) EG26600A (cg-RX-API-DMAC7.html)
ES (1) ES2624776T3 (cg-RX-API-DMAC7.html)
HU (1) HUE031698T2 (cg-RX-API-DMAC7.html)
IL (1) IL202568A (cg-RX-API-DMAC7.html)
MA (1) MA31516B1 (cg-RX-API-DMAC7.html)
MX (1) MX2009013665A (cg-RX-API-DMAC7.html)
NZ (2) NZ598417A (cg-RX-API-DMAC7.html)
PL (1) PL2155880T3 (cg-RX-API-DMAC7.html)
PT (1) PT2155880T (cg-RX-API-DMAC7.html)
RU (2) RU2499053C2 (cg-RX-API-DMAC7.html)
SG (1) SG185249A1 (cg-RX-API-DMAC7.html)
SI (1) SI2155880T1 (cg-RX-API-DMAC7.html)
TN (1) TN2009000512A1 (cg-RX-API-DMAC7.html)
WO (1) WO2008151440A1 (cg-RX-API-DMAC7.html)
ZA (1) ZA201000207B (cg-RX-API-DMAC7.html)

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KR20100120157A (ko) 2007-11-27 2010-11-12 메디카고 인코포레이티드 헤마글루티닌을 발현하는 트랜스제닉 식물에서 생산된 재조합 인플루엔자 바이러스-유사 입자(ＶＬＰｓ)
CN102089432A (zh) 2008-07-08 2011-06-08 麦迪卡格公司 可溶性重组流感抗原
AU2009270404B2 (en) 2008-07-18 2015-01-29 Medicago Inc. New influenza virus immunizing epitope
HRP20180706T1 (hr) 2009-06-24 2018-06-01 Medicago Inc. Kimerna gripa nalik čestici koja sadrži hemaglutinin
JP5551780B2 (ja) 2009-09-22 2014-07-16 メディカゴ インコーポレイテッド 植物由来のタンパク質を調製する方法
TWI620816B (zh) 2011-03-23 2018-04-11 苜蓿股份有限公司 植物衍生蛋白回收方法
US11673926B2 (en) * 2011-06-07 2023-06-13 Ibio, Inc. In vivo de-glycosylation of recombinant proteins by co-expression with PNGase F
US9181532B2 (en) 2011-07-29 2015-11-10 Icon Genetics Gmbh Production of galactosylated N-glycans in plants
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KR20130125337A (ko) * 2012-05-08 2013-11-18 경상대학교산학협력단 고-만노오스형 n-글라이칸을 생산하는 식물체 및 이를 이용한 고-만노오스형 n-글라이칸의 생산방법
EP2978848B1 (en) 2013-03-28 2020-05-06 Medicago Inc. Influenza virus-like particle production in plants
US12467058B2 (en) 2013-03-28 2025-11-11 Aramis Biotechnologies Inc. Influenza virus-like particle production in plants
RU2731295C2 (ru) 2014-07-11 2020-09-01 Медикаго Инк. Модификация продуцирования белков в растениях
EP3221455B1 (en) 2014-11-20 2020-08-12 Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. Compositions and methods for producing polypeptides with a modified glycosylation pattern in plant cells
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Publication number Publication date
NZ581944A (en) 2012-03-30
MX2009013665A (es) 2010-06-02
RU2013137208A (ru) 2015-02-20
WO2008151440A4 (en) 2009-02-26
RU2499053C2 (ru) 2013-11-20
AU2008261527B2 (en) 2014-04-10
JP2010531136A (ja) 2010-09-24
CA2795379A1 (en) 2008-12-18
EP2155880A1 (en) 2010-02-24
TN2009000512A1 (en) 2011-03-31
AU2008261527B8 (en) 2014-04-24
EP2155880A4 (en) 2010-08-04
AU2008261527A1 (en) 2008-12-18
EP2155880B1 (en) 2016-08-10
DK2155880T3 (en) 2016-12-05
SG185249A1 (en) 2012-11-29
BRPI0812538B1 (pt) 2021-03-16
MA31516B1 (fr) 2010-07-01
RU2010101024A (ru) 2011-07-20
BRPI0812538A2 (pt) 2014-09-30
PT2155880T (pt) 2016-11-18
ES2624776T3 (es) 2017-07-17
CA2700180A1 (en) 2008-12-18
KR101385050B1 (ko) 2014-05-13
IL202568A0 (en) 2011-08-01
WO2008151440A8 (en) 2010-03-04
ZA201000207B (en) 2013-09-25
KR20100037602A (ko) 2010-04-09
US20110008837A1 (en) 2011-01-13
PL2155880T3 (pl) 2017-06-30
CN103382487A (zh) 2013-11-06
IL202568A (en) 2015-02-26
CN104962579A (zh) 2015-10-07
AU2008261527A8 (en) 2010-01-21
CA2700180C (en) 2013-01-29
EG26600A (en) 2014-03-23
HUE031698T2 (en) 2017-07-28
JP5783720B2 (ja) 2015-09-24
AU2008261527C1 (en) 2014-09-04
CN101918559A (zh) 2010-12-15
WO2008151440A1 (en) 2008-12-18
NZ598417A (en) 2013-06-28
SI2155880T1 (sl) 2017-03-31

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