KR20090127868A - 광역 전사 기구 조작 - Google Patents

광역 전사 기구 조작 Download PDF

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KR20090127868A
KR20090127868A KR1020097014223A KR20097014223A KR20090127868A KR 20090127868 A KR20090127868 A KR 20090127868A KR 1020097014223 A KR1020097014223 A KR 1020097014223A KR 20097014223 A KR20097014223 A KR 20097014223A KR 20090127868 A KR20090127868 A KR 20090127868A
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South Korea
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ethanol
gene
yeast strain
genetically modified
yeast
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English (en)
Korean (ko)
Inventor
할 에스. 알퍼
그레고리 스테파노파울로스
제럴드 핑크
Original Assignee
메사추세츠 인스티튜트 오브 테크놀로지
화이트헤드 인스티튜트 포 바이오메디칼 리서치
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Publication of KR20090127868A publication Critical patent/KR20090127868A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01009—D-Xylulose reductase (1.1.1.9), i.e. xylitol dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01005—Xylose isomerase (5.3.1.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Ecology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
KR1020097014223A 2006-12-07 2007-12-07 광역 전사 기구 조작 Withdrawn KR20090127868A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US87341906P 2006-12-07 2006-12-07
US60/873,419 2006-12-07

Publications (1)

Publication Number Publication Date
KR20090127868A true KR20090127868A (ko) 2009-12-14

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KR1020097014223A Withdrawn KR20090127868A (ko) 2006-12-07 2007-12-07 광역 전사 기구 조작

Country Status (7)

Country Link
US (1) US20100291648A1 (https=)
EP (2) EP2099910A2 (https=)
JP (1) JP2010512147A (https=)
KR (1) KR20090127868A (https=)
CN (1) CN101743310A (https=)
AU (1) AU2007352393A1 (https=)
WO (1) WO2008133665A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101428191B1 (ko) * 2012-09-24 2014-08-11 이화여자대학교 산학협력단 신규한 에탄올-내성 변이주 및 그의 용도

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JP5804327B2 (ja) * 2009-11-30 2015-11-04 国立大学法人神戸大学 バイオマスからのエタノールの生産方法
JP5585952B2 (ja) * 2009-12-08 2014-09-10 独立行政法人酒類総合研究所 エタノールの製造方法
US8809060B2 (en) * 2010-04-23 2014-08-19 Ewha University-Industry Collaboration Foundation Ethanol-resistant yeast gene, and use thereof
FR2968313B1 (fr) * 2010-12-03 2014-10-10 Lesaffre & Cie Procede de preparation d'une levure industrielle, levure industrielle et application a la production d'ethanol a partir d'au moins un pentose
CN103403143B (zh) * 2011-02-25 2015-11-25 三得利控股株式会社 木糖发酵微生物
WO2012170436A1 (en) * 2011-06-06 2012-12-13 The Regents Of The University Of California Synthetic biology tools
KR101517558B1 (ko) 2012-10-09 2015-05-06 이화여자대학교 산학협력단 고농도 rna 효모 균주 및 그 이용
US20140273131A1 (en) * 2013-03-13 2014-09-18 Abengoa Bioenergy New Technologies, Inc. Methods and systems for use in deactivating organisms used in bioproduct production processes
US20140302577A1 (en) * 2013-04-03 2014-10-09 University Of Wyoming Enhanced yeast fermentation platform using yeast lacking mitochondrial dna and containing growth improving mutations
KR101646310B1 (ko) * 2013-06-24 2016-08-12 씨제이제일제당 (주) L-쓰레오닌 생산 미생물 및 이를 이용한 l-쓰레오닌의 생산방법
CN105907779B (zh) * 2016-05-25 2019-07-09 西北农林科技大学 一种可降低酿酒酵母乙醇产率的重组质粒pY16TEF1-△SPT15及其应用
CN105950649B (zh) * 2016-05-25 2019-07-09 西北农林科技大学 一种可提高酿酒酵母乙醇产率的重组质粒pY16TEF1-△SPT15-125及其应用
CN106434700B (zh) * 2016-08-19 2019-05-03 南阳师范学院 一种提高乙醇产率的酿酒酵母spt15定点饱和基因突变方法
CN106978380A (zh) * 2016-12-14 2017-07-25 天津科技大学 一株高木糖耐性肺炎克雷伯氏菌株及其构建方法
CN108559721A (zh) * 2018-05-15 2018-09-21 北京师范大学 一种净化空气的复合微生物菌剂及其应用
EP4012015A1 (en) * 2020-12-14 2022-06-15 Sekab E-Technology AB Method and system for control of a microbial fermentation process
WO2023092547A1 (zh) * 2021-11-29 2023-06-01 中国科学院深圳先进技术研究院 基于自动化技术构建的高附加值代谢物智能细胞工厂
CN116179450A (zh) * 2021-11-29 2023-05-30 中国科学院深圳先进技术研究院 基于自动化技术构建的高附加值代谢物智能细胞工厂
CN120249166B (zh) * 2025-06-06 2025-10-03 北京化工大学 一种产1,3-丁二醇的基因工程菌及其应用

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101428191B1 (ko) * 2012-09-24 2014-08-11 이화여자대학교 산학협력단 신규한 에탄올-내성 변이주 및 그의 용도

Also Published As

Publication number Publication date
CN101743310A (zh) 2010-06-16
US20100291648A1 (en) 2010-11-18
AU2007352393A1 (en) 2008-11-06
EP2099910A2 (en) 2009-09-16
EP2407541A1 (en) 2012-01-18
WO2008133665A3 (en) 2009-06-25
JP2010512147A (ja) 2010-04-22
WO2008133665A2 (en) 2008-11-06

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Patent event date: 20090707

Patent event code: PA01051R01D

Comment text: International Patent Application

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PC1203 Withdrawal of no request for examination
WITN Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid