KR20090091541A - Composition from natural origin ingridient for hair loss prevention and hair growth effect - Google Patents

Abstract

A composition for preventing hair loss and promoting hair growth is provided to increase biosynthesis of collagen in scalp and suppress androgenic to scalp. A composition for preventing hair loss and promoting hair growth comprises: 1~5 weight% of concentrate having avocado fruit and elder fruit in a weight ratio of 1:3~3:1; and 10-50 weight% of matured solution of dictamni Radicis Cortx, dianthi Herba, Magnolia Bud, walnut tree, flower of the sweet false chamomile, four frame, the rhizoma of ivy, and horsetail.

Description

Hair Loss Prevention and Hair Regrowth Composition using Natural Sources {COMPOSITION FROM NATURAL ORIGIN INGRIDIENT FOR HAIR LOSS PREVENTION AND HAIR GROWTH EFFECT}

The present invention relates to a composition having a hair loss prevention and hair regrowth function using natural resources, and more particularly, to normalize the physiological state by inhibiting collagen biosynthesis in the scalp, and to inhibit the male hormone on the scalp tissue, and at the same time the scalp and The present invention relates to a composition having a hair loss prevention and hair growth function using natural derived resources having the effects of preventing hair loss and promoting hair growth as well as improving hair condition.

In general, the human hair is about 100,000 to 150,000 pieces, each hair has a different cycle and grow, drop out through the anagen, catagen, telogen. This cycle is repeated over three to six years, with the result that on average 50-100 hairs fall out normally. In general, alopecia refers to a shortage of hair growth in the growth phase and more hair in the degenerative or maintenance phase of these cycles, resulting in an abnormal number of hairs falling out.

The causes of hair loss include excessive male hormone action, excessive sebum secretion, poor blood circulation, peroxide, bacteria, deterioration of scalp function, hypoxic collagen production, genetic factors, aging and stress. Has been.

However, to date, no clear cause of hair loss is known, and in recent years, more and more people are suffering from hair loss due to changes in diet and stress due to social conditions. It is increasing.

Conventionally, in the treatment or prevention of such alopecia, it is a formulation mainly composed of female hormone for promoting blood circulation, strengthening of hair follicles, scalp moisturizing, and suppressing male hormones, or high blood pressure medicine that regulates blood pressure. While developed minoxidil and prostatic hyperplasia are used for the treatment of prostates, etc., which are focused on the side effects, are sold, but there are problems of side effects such as loss of energy, and if they are discontinued, their efficacy or effectiveness cannot be sustained. There are drawbacks to returning to the state, so there is no clear effect so far, and hair transplantation methods are expensive and aspects such as care, hair roots and hair follicles cannot be activated. In addition, while well-being winds are being developed, products using herbal and plant extracts have been developed, but products using these plant extracts are commonly exposed to problems such as skin irritation due to preservatives used to prevent corruption of extracts. .

The present invention has been made to solve the above problems, the object of the present invention in view of the problems described above using a conventional hair loss preventing effect or hair growth efficacy, and the like, male hormone associated with alopecia It has the functions of preventing hair loss and hair growth by using natural resources that inhibits the action, nourish the scalp and hair related to hair growth, prevents scalp itching and dandruff, prevents hair loss of non-preservatives without skin irritation and promotes hair growth. It is to provide a composition.

The purpose is 1 to 5% by weight of the concentrated fermentation broth concentrated and fermented by mixing avocado fruit and elder fruit in a weight ratio of 1: 3 to 3: 1 with respect to the total weight of the composition, cheoncho, ringworm skin, guru, xinhua, walnut tree Hair loss prevention and hair growth function using natural derived resources, characterized in that it comprises 10 to 50% by weight of the aging liquid of one or more aging ingredients selected from the group consisting of leaves, flowers of camomile, nettle, root of ivy, hosttail, etc. It is achieved by a composition having a.

Here, the concentrated fermentation broth is a pulverized pulverized crushed whole of the avocado fruit and elder fruit, flesh and seeds at 20 ~ 30 ℃ for 10 to 13 days, and then filtered filtrate continuously at a constant temperature of 15 ℃ It is characterized in that the concentrated fermentation solution concentrated under reduced pressure so as to mature for 30 days and the concentration of the solid is 20 to 30% by weight.

In addition, the aging liquid is a aging liquid after the extraction of the aging component with water for 48 hours, the first aging and filtering for 10 days, and then put the jade in the first filtration aging liquid for a second deodorizing aging process for 5 days do.

Preferably, the aging solution is characterized in that the aging solution is added to the mixture of 1 to 5% by weight of deep sea water to the total weight of the aging solution.

In addition, the composition having a hair loss prevention and hair growth function using the natural derived resources is characterized in that it further comprises 0.1% by weight to 1.0% by weight of the liposome nano-liposomalized aspartic acid liposome.

In addition, the astaxanthin liposome is 4.0% by weight of phospholipid, 0.1% by weight of glutathione, 4.0% by weight of polysorbate 20, 20% by weight of 95% modified ethanol, 30% by weight of glycerin, 1.0-5.0% by weight of astaxanthic acid and the balance It is characterized in that the nanoliposome prepared by high-pressure emulsification using purified water.

According to the present invention, the cosmetic industry by remarkably excellent collagen biosynthesis in the scalp, normalizing the physiological state by inhibiting male hormones on the scalp tissue, and at the same time improving the scalp and hair condition as well as preventing hair loss and promoting hair growth. This is very useful.

Hereinafter, the present invention will be described in detail with reference to embodiments and drawings of the present invention. These examples are only presented by way of example only to more specifically describe the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples. .

The composition having a hair loss prevention and hair growth function using natural derived resources according to the present invention is a concentrated fermentation solution 1 ~ 5% by weight of fermented avocado and elder fruit by mixing in a weight ratio of 1: 3 to 3: 1 to the total weight of the composition 10 to 50% by weight of the ripening liquid of one or more ripening ingredients selected from the group consisting of cheoncho, ringworm, veins, xinhua, walnut leaves, flowers of camomile, nettle, root of ivy, hosttail, etc. It is characterized by.

Fermentation and maturation of the elder, which is a deciduous broad-leaved shrub of the avocado and the locust tree, allows the extraction of significant amounts of active ingredients such as flavonoids, vitamins and potassium in avocados and elders, and any other solvents. Even without the use of excellent hair loss prevention and hair growth effect can be obtained. In the present invention, the avocado fruit and the elder fruit were mixed and used in a weight ratio of 1: 3 to 3: 1, and the concentrated fermentation and fermentation were carried out using the whole skin, pulp and seed of the avocado fruit and the elder fruit without using a solvent. The fermentation broth is extracted and used.

The concentrated fermentation broth is fermented at 20-30 ℃ for 10-13 days pulverized pulverized avocado and elder bark, flesh and seeds (ie, fruit itself), and the filtrate filtered filtrate at a constant temperature of 15 ℃ After aging for 30 days continuously and concentrated under reduced pressure so that the concentration of solids 20 ~ 30% to produce a concentrated fermentation solution. If the fermentation conditions are 10 to 13 days at 20 ~ 30 ℃ is related to the fermentation capacity of the fruit, if the fermentation period is too long when less than 20 ℃, fermentation is stopped by over fermentation if it exceeds 30 ℃ Because there is a danger. Therefore, fermentation is completed by fermentation at the temperature for 10 to 13 days. Once fermentation is complete, avoid direct sunlight and continuously aging for 30 days at a constant temperature of 15 ℃ without changing the temperature. This is because fermentation broth being aged is easily oxidized when subjected to direct sunlight, and the fermentation broth has a characteristic of changing physical properties well according to time, temperature and light. In other words, if the temperature is too low, the aging is not properly, if the temperature is too high, it is not suitable for aging for a long time, so for the extraction of the active ingredient, it is desirable to keep the aging temperature to 15 ℃ continuously, the period is aged for 30 days It is preferable. Through this aging step, the naturally occurring alcohol during fermentation is mild to prevent the risk of causing skin irritation due to alcohol and to obtain an aromatic extract. Next, the extract after the aging process is concentrated under reduced pressure until the solid concentration of the extract is about 20 to 30% by weight, which eliminates the threat of the stability of the formulation by adding an excessive amount of the unconcentrated extract when preparing the composition. Even if a small amount of concentrated fermentation extract is added to the total weight of the composition, the present invention is intended to exhibit hair loss prevention and hair regrowth effect.

The amount of the concentrated fermentation extract is preferably 1 to 5% by weight based on the total weight of the composition, when less than 1% by weight is not satisfactory the effect of hair loss prevention and hair growth, when the content exceeds 5% by weight to prevent hair loss And the increase in the effect of hair growth is insignificant and uneconomical in terms of product stability.

In addition, the composition having a hair loss prevention and hair growth function using the natural derived resources according to the present invention is selected from the group consisting of cheoncho, baekryepi, veins, myths, leaves of walnut, flowers of camomile, nettle, root of ivy, hosttail, etc. Aging liquid of one or more selected maturation ingredients.

Herbs used in the aging solution is used synergistically synergistically with the concentrated fermentation solution, the herb is heated and extracted with water for 48 hours, the first ripening for 10 days and filtered, and then in the first filtration aging solution It is preferable to prepare a aging liquid after jade is subjected to secondary deodorization aging process for 5 days. The primary filtration aging solution is added to jade to remove the coarse reverberation of the herb that does not disappear after 10 days of aging. Jade emits far-infrared rays, which has the effect of sterilization and anti-corruption during aging and deodorizing effect (AESPRO, March 2006 issue 12P).

The amount of the aging liquid is preferably 10 to 50% by weight based on the total weight of the composition, and when the amount is less than 10% by weight, there is a disadvantage in that the efficacy is expressed as a composition. This is because there is a slight increase in the effects of hair loss prevention and hair growth and it is uneconomical in terms of product stability.

More preferably it is a matured liquid prepared by adding 1-5% of deep sea water to the total weight of the matured liquid. This is because the salt contained in the deep sea water contributes to the stability of the aging liquid by acting as a natural preservative. If the amount of deep sea water is less than 1% by weight of the total weight of the aging liquid, it does not play a role in preservation. If the amount of the deep sea water exceeds 5% by weight, it is exposed to the side effect of chelation when preparing the composition, which may cause a risk of stability of the product. do.

On the other hand, astaxanthin is a red pigment contained in crustaceans and salmon and contributes to anti-inflammatory, arteriosclerosis, immune regeneration, diabetes control, lowering blood pressure, and improving eye function. In addition, it is an excellent ingredient that removes harmful free radicals that cause aging and cancer.

The composition having a hair loss prevention and hair regrowth function using natural derived resources according to the present invention is 0.1 wt% to 1.0% astaxanthin liposome with respect to the total weight of the composition in order to increase the mutual efficacy with the fermentation concentrate and the aging solution. It is preferable to further contain the weight%. The astaxanthin liposomes are nanoliposomes that have been reduced in size by passing a liposome consisting of astaxanthic acid, phospholipids, glutathione, polysorbate 20, glycerin and ethanol seven times through a high pressure emulsifier. The amount of the astaxanthic acid liposome is preferably used in an amount of 0.1% by weight to 1.0% by weight in the total weight of the composition, but less than 0.1% by weight does not have a synergistic effect of the fermentation concentrate and the aging liquid, when it exceeds 1.0% by weight This is because the mutual synergistic effect is insignificant compared to the amount used, which is uneconomical.

In addition, the astaxanthin liposome is 4.0% by weight of phospholipid, 0.1% by weight of glutathione, 4.0% by weight of polysorbate 20, 20% by weight of 95% modified ethanol, 30% by weight of glycerin, 1.0-5.0% by weight of astaxanthic acid and the balance It is preferable to prepare using purified water.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. The following examples are only for illustrating the present invention, but the scope of the present invention is not limited thereto.

EXAMPLE

[Production Example 1]

Avocado fruit and elder fruit (shell, pulp, seed) were weighed and ground in a 1: 1 ratio, and then put in a brewing container and fermented at 25 ° C. for 11 days, followed by filtration with 200 mesh filter cloth (Chang-Gye). Aged at constant temperature for 30 days. The matured product was concentrated under reduced pressure until the concentration of solids was concentrated to about 20-30% by a Tokyo Rikakikai Co., Rorary vacuum evaporator to obtain a concentrated fermentation solution.

[Production Example 2]

20 kg of Shin-Ihwa was added to 100 kg of water and extracted by heating for 48 hours with primary fermentation and filtration for 10 days. After jade was added to the primary filtrating aging solution for 5 days, the deodorizing aging process was carried out for 5 days to obtain 35 kg of aging liquid.

[Production Example 3]

700 g of deep sea water was further added to the aging liquid obtained in the same manner as in Preparation Example 2, and mixed to obtain a aging liquid to which the deep sea water was added.

[Production Example 4]

Add 10 kg of Shin-Ihwa and 10 kg of gut into 100 kg of water, extract the mixture with water for 48 hours, and then ferment for 10 days. After filtration, add jade to the primary filtration solution for 5 days and deodorize aging for 5 days. Got.

Production Example 5

740 g of deep seawater was further added to the aging solution obtained in the same manner as in Preparation Example 4, and mixed to obtain a aging solution to which the deep seawater was added.

[Manufacture example 6]

Nanoliposomes are made by reducing the size of the liposome consisting of phospholipid, glutathione, ethanol, glycerin and polysorbate 20 through a high-pressure emulsifier seven times. Phospholipids, glutathione, 95% modified ethanol, glycerin, and polysorbate 20 were emulsified under high pressure to form a closed bilayer nanoliposome. The phase A of the following Table 1 was heated up to 95 degreeC, and it mixed and dissolved uniformly. It was cooled to 40 ° C and phase B was mixed. Next, the resultant was passed through a high-pressure emulsifier (Microfludizer M210EH, Microfluidies, USA) seven times under a pressure of 1200 bar and a flow rate of 500 m / s to obtain astaxanthic acid liposomes. The size of the liposome obtained through one pass was about 133 nm, but the size of the seventh was about 75 nm.

TABLE 1

phase Raw material name Content (% by weight) A Phospholipids 4 Glutathione 0.1 95% modified ethanol 20 glycerin 30 Polysorbate 20 4 B Astaxanthin One Purified water Remaining amount system   100

[Manufacture example 7]

Astaxanthin liposomes were obtained in the same manner as in Preparation Example 6 except that the content of astaxanthin acid was changed to 5% by weight in Preparation Example 6.

[Examples 1 to 5 and Comparative Example 1]

The hair tonic was prepared according to a conventional manufacturing method using the composition of Table 2, which was set as Examples 1 to 5 and Comparative Example 1.

TABLE 2

Ingredient Name Example 1 Example 2 Example 3 Example 4 Example 5 Comparative Example 1 A HCO-60 2.0 2.0 2.0 2.0 2.0 2.0 Crillet 3 0.5 0.5 0.5 0.5 0.5 0.5 Crillet 1 0.5 0.5 0.5 0.5 0.5 0.5 B EtOH 1.0 1.0 1.0 1.0 1.0 1.0 Fragrance Quantity Quantity Quantity Quantity Quantity Quantity C Concentrated fermentation solution of Preparation Example 1 1.0 1.0 1.0 1.0 1.0 1.0 Aging liquid of Preparation Example 2 10 - - - 10 10 Aging liquid of Preparation Example 3 - 10 - - - - Aging liquid of Preparation Example 4 - - 10 - - - Aging liquid of Preparation Example 5 - - - 10 - - Nanosome of Preparation Example 6 1.0 1.0 1.0 1.0 - - Nanosome of Preparation Example 7 - - - - 0.1 - D Sodium Citrate 0.6 0.6 0.6 0.6 0.6 0.6 Purified water Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Sum 100.0 100.0 100.0 100.0 100.0 100.0

[Examples 6 to 10 and Comparative Example 2]

To prepare a hair shampoo according to a conventional manufacturing method using the composition of Table 3, it was set as Examples 6 to 10 and Comparative Example 2.

TABLE 3

Ingredient Name Example 6 Example 7 Example 8 Example 9 Example 10 Comparative Example 2 A Micolin ES-430 25.00 25.00 25.00 25.00 25.00 25.00 Micolin S-430 15.00 15.00 15.00 15.00 15.00 15.00 Micopol CDE 3.60 3.60 3.60 3.60 3.60 3.60 Glycerin 1.40 1.40 1.40 1.40 1.40 1.40 B Purified water Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount P.G 1.00 1.00 1.00 1.00 1.00 1.00 EDTA-2Na 0.20 0.20 0.20 0.20 0.20 0.20 Germall 115 0.10 0.10 0.10 0.10 0.10 0.10 C Concentrated fermentation solution of Preparation Example 1 1.0 1.0 1.0 1.0 1.0 1.0 Aging liquid of Preparation Example 2 10 - - - 10 10 Aging liquid of Preparation Example 3 - 10 - - - - Aging liquid of Preparation Example 4 - - 10 - - - Aging liquid of Preparation Example 5 - - - 10 - - Nanosome of Preparation Example 6 1.0 1.0 1.0 1.0 - - Nanosome of Preparation Example 7 - - - - 0.1 - D incense Quantity Quantity Quantity Quantity Quantity Quantity Sum 100.0 100.0 100.0 100.0 100.0 100.0

[ Experimental Example  One] 5 alpha - Reductase  Activity inhibitory test

5α-reductase was used in 5 alpha (α) -reductase activity inhibition experiments in rat liver (adult, Sprague-Dawley, male rat (8-10weeks old), liver), An amount of protein suspension was prepared. In addition, an enzyme reaction solution was prepared in a general manner. Radiolabeled testosterone (3Htestosterone) was used as a substrate of 5α-reductase, and enzyme reaction solution: protein suspension: extract = 70: 20: 10 incubator at a ratio of 2 minutes. After the reaction was carried out, the reaction solvent was blown out, the remaining material was dissolved in the developing solvent, developed on a thin layer chromatography (TLC), and the conversion rate to the developed testosterone was measured. 5α-reductase inhibition rate was evaluated. The inhibition rate calculation method is shown in Equation 1 below.

[Equation 1]

% Inhibition = [(A-B) / A] x 100

Where A = conversion of testosterone to dihydrotestosterone (when no extract is added) and B = conversion of testosterone to dihydrotestosterone (when extract is added).

Experimental results by the inhibition rate calculation method are shown in Tables 4 to 6.

[Table 4] 5α-reductase activity inhibitory activity as Preparation Example 1

Concentrated Fermentation Liquid Concentration 0.5% One% 5% 6% Inhibition (%) 60.2 87.2 100 100

TABLE 5 5α-reductase activity inhibitory power as Preparation Example 2

Aging liquid concentration 5% 10% 30% 50% Inhibition (%) 38.5 86.7 98.5 100

[Table 6] Inhibitory Activity of 5α-Reductase Activity as Preparation Example 4

Aging liquid concentration 5% 10% 30% 50% Inhibition (%) 42.3 87.1 97.6 100

In order to evaluate 5α-reductase activity inhibitory evaluation test for the Preparation Example 1, Preparation Example 2, Preparation Example 4, 5α-reductase activity inhibitory power was evaluated by the method described below. The inhibition rate calculation method is shown in Equation 2 below.

[Equation 2]

% Inhibition = [(A-B) / A] x 100

Where A = testosterone to dihydrotestosterone conversion (40 wt% aqueous ethanol solution), B = testosterone to dihydrotestosterone conversion (examples and comparative examples), 5α-reductase activity inhibitory power is 80 It is judged to be effective if there is more than% result.

Examples 1 to 5 and Comparative Example 1 of the hair tonic 5α-reductase activity inhibitory test results are shown in Table 7.

Table 7 5α-reductase activity inhibitory power of the hair tonic of Examples 1 to 5 and Comparative Example 1

division Example 1 Example 2 Example 3 Example 4 Example 5 Comparative Example 1 Inhibition (%) 100 100 100 100 100 95.5

As can be seen from the results according to Table 7, the effects of the present invention can be seen in all examples of the composition having a hair loss prevention and hair growth function using natural resources according to the present invention. In addition, as can be seen in Examples 1 to 5 and Comparative Example 1, it can be seen that the asanthanthic acid liposome causes the synergy between the fermentation concentrate and the aging solution.

Experimental Example 2 Test for Inhibiting Dandruff Growth

Growth of Pityrosporium ovale (hereinafter referred to as P. ovale), which is a dandruff bacterium present in the hair and scalp for the Examples 1 to 5 and Comparative Example 1, which causes nutrients of hair follicles to grow as a food thereof and causes hair loss. Inhibitory ring tests were performed. The strain was purchased from a strain of ATCC14521 from the cell line bank. Inoculate P. ovale bacteria to the previously prepared dandruff bacteria medium and incubate at 37 ° C. for 24-48 hours. After incubation, P. ovale bacteria are concentrated in dilute solution using platinum, and a suspension of 108 CFU / ml is prepared. Inoculate the bacterial suspension evenly into the dandruff culture medium using a sterile swab and place a sterile disk in the center of the medium. 60 μl of the sample diluted to 50% using hard water is dropped onto the disc, and then incubated at 37 ° C. for 48 hours. After measuring the total diameter of the sample and the low zone, the growth zone (Inhibition zone; low ring) was obtained by the following Equation 3.

[Equation 3]

Inhibition Zone (W) = (T-D) / 2

W: width of growth zone (mm)

T: total diameter of sample and low zone (mm)

D: diameter of sample (mm)

As a result of the antifungal effect of Examples 1 to 5 and Comparative Example 1 P. known as dandruff. The ovale showed a high antifungal effect of about 12.35 mm, and the results are shown in Table 8 and FIG. 1.

Table 8 Antifungal Test Results

Sample classification Sample inoculation amount Disk diameter Width of growth zone Example 1 60 μl 8.0mm 12.5mm Example 2 60 μl 8.0mm 12.3mm Example 3 60 μl 8.0mm 12.4mm Example 4 60 μl 8.0mm 12.4mm Example 5 60 μl 8.0mm 12.5mm Comparative Example 1 60 μl 8.0mm 12.0mm

Experimental Example 3 Collagen Biosynthesis Effect Experiment

The collagen biosynthesis effect was tested using the hair tonic of Examples 1 to 5 and Comparative Example 1. First, fibroblasts cultured in a T-75 flask were washed 2-3 times with PBS, and the attached cells were dropped using trypsin, and then cells were collected by centrifugation (Heraeus; Labofuge 400R) at 1500 rpm for 5 minutes. After centrifugation, the supernatant is discarded and 10 ml of DMEM (10% FBS) is added to the collected cells and mixed evenly. Measure the number of cells using a hemocytometer and dispense 200ea of cells about 96ea / well into each well of 96well. After culturing at 37 ° C and 5% CO2, the cells grow to about 50% of the well area and exchange them with fresh DMEM (FBS10%).

Collagen synthesis performance measurement kit (Procollagen Type-I C-peptide EIA kit (Takara, MK 101)) was used. For measurement using an immunological method, transfer 100 μl of Ab-POD conjugate solution to one well and dispense, and add 20 μl of cell culture solution or standard solution. Wrap 96well plates with foil or the like and incubate at 37 ℃ for 3 hours. After 3 hours, remove all the solution from each well and wash 4 times with PBS. After the washing step, 100 μl of substrate solution is added to each well and incubated at 20-30 ° C. for 15 minutes. In the final step, add 100µl of the reaction solution 1N H2SO4 and mix gently. The absorbance was measured using an ELISA leader, and the result was used. The plate containing the reaction termination solution was stored at room temperature for about 1 hour and then measured at 450 nm.

The results are shown in Figure 2 and can be seen that the enhancement effect is shown as well as the results of cell proliferation ability. In addition, as can be seen in Examples 1 to 5 and Comparative Example 1, it can be seen that the asanthanthic acid liposome causes the synergy between the fermentation concentrate and the aging solution.

Experimental Example 4 Experiment for Confirming the Effect of Hair Growth Using In-vivo Evaluation Method

The hair tonic of Example 1 and the hair shampoo composition of Example 6 were applied to 10 people with severe hair loss from all over the country, after shampooing once a day, after towel dry, hair tonic was evenly applied to the scalp and the hair was massaged. The results were observed after 3 months of use with nothing applied. The results are shown in Table 9 and FIGS. 3 to 5 (only three of ten are examples), and it can be seen that the hair growth effect is large.

Table 9 Results of confirming hair growth effect

Symptom Before use 3 months after use Hair growth effect Ten people worry about hair loss 10 hair growth effects Skin irritation or side effects none none

With 10 subjects, the hair loss effect was remarkably reduced in all 10 patients. There were no skin irritation or side effects for 3 months. 3 to 5 are photographs showing the results for three out of ten subjects.

Figure 3a is subject 1 (male, 38 years old, hair loss progressed from 2002, stress-free hair loss, no baldness in the family, the specificity is stress-sensitive and renal function markedly off, especially after hair loss begins to be treated or Hair loss and hair loss products were not used.) Is a picture taken the hair, Figure 3b is the hair after 3 months of subject 1 using a composition having a hair loss prevention and hair growth function using natural resources according to the present invention. This picture was taken.

Figure 4a is subject 2 (male, 23, frontal hair loss, alopecia hair loss symptoms from a young age, full-scale hair loss from the 3rd year of high school, no balds in the family, the specifics have never had a big disease, but the body Extremely fragile, stress sensitive and markedly intestinal debilitating; after 1 year of hair loss, H admitted to the H hospital for medications and radiofrequency treatment for a year, after which the scalp began to turn red, and the scalp became red. Figure 4 is a photograph of the hair, Figure 4b is a photograph of the hair after 3 months of subject 2 using the composition having a hair loss prevention and hair growth function using natural resources according to the present invention.

Figure 5a is the subject 3 (male, 33 years old, male type baldness, hair loss starting from about 10 years ago, only the family and their father's hair loss, the specificity of the scalp is oily and powerless hair, 2005 in the hospital Since January, I have received scalp injections (once a week), topical medications, and oral medications for more than 3 months, but no progress has been made. The scalp was oily and negative when tested for Demodex, and the microscopic findings of the scalp were highly flaky and hairy. This was a very thin.) Is a photograph of the hair, Figure 5b is a photograph of the hair after 3 months of the subject 3 using a composition having a hair loss prevention and hair growth function using natural resources according to the present invention. .

These results suggest that the shampoo and hair tonic composition according to the present invention exhibits a reduction in male hormones in the scalp, collagen biosynthesis and antioxidant, and anti-inflammatory effects, thereby preventing hair loss and promoting hair growth. . In addition, it has been confirmed that the composition of the present invention does not cause irritation or skin trouble even when used for a long time.

1 is a photograph of the results of testing the growth inhibition of P. ovale bacteria for Preparation and Comparative Preparation Examples.

2 is a collagen biosynthesis comparison graph.

Figure 3a is a photograph of the hair of the subject 1

Figure 3b is a photograph of the hair after 3 months of subject 1 using the composition having a hair loss prevention and hair growth function using the natural derived resources according to the present invention.

Figure 4a is a photograph of the hair of the subject 2

Figure 4b is a photograph of the hair after 3 months of subject 2 using a composition having a hair loss prevention and hair growth function using natural derived resources according to the present invention.

Figure 5a is a photograph of the hair of the subject 3

Figure 5b is a photograph of the hair after three months of the subject 3 using the composition having a hair loss prevention and hair growth function using natural derived resources according to the present invention.

Claims (6)

In the composition having a hair loss prevention and hair growth function using natural derived resources, 1 to 5% by weight of the concentrated fermentation broth mixed with avocado fruit and elder fruit in a weight ratio of 1: 3 to 3: 1 based on the total weight of the composition, It contains 10 to 50% by weight of the aging solution of one or more ripening ingredients selected from the group consisting of Cheoncho, ringworm, veins, xinhua, leaves of walnut tree, flowers of camomile, nettle, root of ivy, hosttail, etc. A composition having a hair loss prevention and hair growth function using natural derived resources. The method of claim 1, The concentrated fermentation broth is fermented at 20 ~ 30 ℃ for 10-13 days the pulverized pulverized avocado and elder fruit peels, pulp and seeds, and then filtered filtrate continuously at a constant temperature of 15 ℃ 30 days A composition having a hair loss prevention and hair growth function using natural derived resources, characterized in that it is a concentrated fermentation solution concentrated under reduced pressure so that the concentration of the aging and solids is 20 to 30% by weight. The method of claim 1, The aging liquid is a aging liquid after the extraction of the aging component with water for 48 hours, the first aging for 10 days and filtered, and then put the jade in the first filtration aging liquid for 2 days deodorizing aging process , A composition having a hair loss prevention and hair growth function using natural derived resources. The method of claim 3, The aging solution is a aging solution, characterized in that the addition of 1 ~ 5% by weight of deep sea water to the total weight of the aging solution, characterized in that the composition having a hair loss prevention and hair growth function using natural resources. The method according to any one of claims 1 to 4, The composition having a hair loss prevention and hair growth function using the natural derived resources further comprises 0.1% by weight to 1.0% by weight based on the total weight of the composition liposomes nanoliposomal, hair loss prevention using natural derived resources And a composition having a hair growth function. The method of claim 5, The astaxanthic acid liposome is composed of 4.0% by weight of phospholipid, 0.1% by weight of glutathione, 4.0% by weight of polysorbate 20, 20% by weight of 95% modified ethanol, 30% by weight of glycerin, 1.0-5.0% by weight of astaxanthic acid and the remaining amount of purified water. A nanoliposome prepared by high pressure emulsification using, characterized in that the composition having a hair loss prevention and hair growth function using natural derived resources.

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