KR20070073932A - 줄기 세포 유래 혈소판 - Google Patents
줄기 세포 유래 혈소판 Download PDFInfo
- Publication number
- KR20070073932A KR20070073932A KR1020077011858A KR20077011858A KR20070073932A KR 20070073932 A KR20070073932 A KR 20070073932A KR 1020077011858 A KR1020077011858 A KR 1020077011858A KR 20077011858 A KR20077011858 A KR 20077011858A KR 20070073932 A KR20070073932 A KR 20070073932A
- Authority
- KR
- South Korea
- Prior art keywords
- platelets
- cells
- human
- megakaryocytes
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 210000003593 megakaryocyte Anatomy 0.000 claims abstract description 72
- 210000003958 hematopoietic stem cell Anatomy 0.000 claims abstract description 28
- 238000000338 in vitro Methods 0.000 claims abstract description 18
- 210000001671 embryonic stem cell Anatomy 0.000 claims abstract description 17
- 210000004027 cell Anatomy 0.000 claims description 91
- 238000000034 method Methods 0.000 claims description 54
- 230000015572 biosynthetic process Effects 0.000 claims description 18
- 210000002242 embryoid body Anatomy 0.000 claims description 16
- 238000012258 culturing Methods 0.000 claims description 11
- 210000004369 blood Anatomy 0.000 claims description 10
- 239000008280 blood Substances 0.000 claims description 10
- 230000035755 proliferation Effects 0.000 claims description 9
- 210000000130 stem cell Anatomy 0.000 claims description 9
- 210000002536 stromal cell Anatomy 0.000 claims description 9
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 claims description 8
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 claims description 8
- 210000002966 serum Anatomy 0.000 claims description 8
- 101001078143 Homo sapiens Integrin alpha-IIb Proteins 0.000 claims description 6
- 102100025306 Integrin alpha-IIb Human genes 0.000 claims description 6
- 108060003951 Immunoglobulin Proteins 0.000 claims description 5
- 102000000646 Interleukin-3 Human genes 0.000 claims description 5
- 108010002386 Interleukin-3 Proteins 0.000 claims description 5
- 102000004889 Interleukin-6 Human genes 0.000 claims description 5
- 108090001005 Interleukin-6 Proteins 0.000 claims description 5
- 102000018358 immunoglobulin Human genes 0.000 claims description 5
- 229940076264 interleukin-3 Drugs 0.000 claims description 5
- 229940100601 interleukin-6 Drugs 0.000 claims description 5
- 101001015004 Homo sapiens Integrin beta-3 Proteins 0.000 claims description 4
- 101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 claims description 4
- 102100032999 Integrin beta-3 Human genes 0.000 claims description 4
- 102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 claims description 4
- 102100031585 ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 Human genes 0.000 claims description 3
- 102000006354 HLA-DR Antigens Human genes 0.000 claims description 3
- 108010058597 HLA-DR Antigens Proteins 0.000 claims description 3
- 101000777636 Homo sapiens ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 Proteins 0.000 claims description 3
- 101001008874 Homo sapiens Mast/stem cell growth factor receptor Kit Proteins 0.000 claims description 3
- 101001071312 Homo sapiens Platelet glycoprotein IX Proteins 0.000 claims description 3
- 101001070790 Homo sapiens Platelet glycoprotein Ib alpha chain Proteins 0.000 claims description 3
- 102100027754 Mast/stem cell growth factor receptor Kit Human genes 0.000 claims description 3
- 102100036851 Platelet glycoprotein IX Human genes 0.000 claims description 3
- 102100034173 Platelet glycoprotein Ib alpha chain Human genes 0.000 claims description 3
- 230000004071 biological effect Effects 0.000 claims description 2
- 230000023555 blood coagulation Effects 0.000 claims description 2
- 102000003815 Interleukin-11 Human genes 0.000 claims 1
- 108090000177 Interleukin-11 Proteins 0.000 claims 1
- 239000000427 antigen Substances 0.000 claims 1
- 102000036639 antigens Human genes 0.000 claims 1
- 108091007433 antigens Proteins 0.000 claims 1
- 230000000977 initiatory effect Effects 0.000 claims 1
- 229940074383 interleukin-11 Drugs 0.000 claims 1
- 229960000182 blood factors Drugs 0.000 abstract 1
- 230000008569 process Effects 0.000 description 25
- 230000004069 differentiation Effects 0.000 description 19
- 210000002381 plasma Anatomy 0.000 description 12
- 239000002243 precursor Substances 0.000 description 10
- 230000003394 haemopoietic effect Effects 0.000 description 9
- 108010047303 von Willebrand Factor Proteins 0.000 description 9
- 102100036537 von Willebrand factor Human genes 0.000 description 9
- 229960001134 von willebrand factor Drugs 0.000 description 9
- 241000699666 Mus <mouse, genus> Species 0.000 description 8
- 241000699670 Mus sp. Species 0.000 description 7
- 108010049003 Fibrinogen Proteins 0.000 description 6
- 102000008946 Fibrinogen Human genes 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 108090000631 Trypsin Proteins 0.000 description 6
- 102000004142 Trypsin Human genes 0.000 description 6
- 229940012952 fibrinogen Drugs 0.000 description 6
- 239000008187 granular material Substances 0.000 description 6
- 239000012588 trypsin Substances 0.000 description 6
- 102100023472 P-selectin Human genes 0.000 description 5
- 238000004113 cell culture Methods 0.000 description 5
- 239000000356 contaminant Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 230000035800 maturation Effects 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 102000008186 Collagen Human genes 0.000 description 4
- 108010035532 Collagen Proteins 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 4
- 108090000190 Thrombin Proteins 0.000 description 4
- 229920001436 collagen Polymers 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 102000005962 receptors Human genes 0.000 description 4
- 108020003175 receptors Proteins 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 229960004072 thrombin Drugs 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 description 3
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 3
- 101000622137 Homo sapiens P-selectin Proteins 0.000 description 3
- 230000002776 aggregation Effects 0.000 description 3
- 238000004220 aggregation Methods 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000003501 co-culture Methods 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 239000003102 growth factor Substances 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 238000012606 in vitro cell culture Methods 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 210000000265 leukocyte Anatomy 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- ZOOGRGPOEVQQDX-UUOKFMHZSA-N 3',5'-cyclic GMP Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=C(NC2=O)N)=C2N=C1 ZOOGRGPOEVQQDX-UUOKFMHZSA-N 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 102000016359 Fibronectins Human genes 0.000 description 2
- 108010067306 Fibronectins Proteins 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 101000621371 Homo sapiens WD and tetratricopeptide repeats protein 1 Proteins 0.000 description 2
- 101000892274 Human adenovirus C serotype 2 Adenovirus death protein Proteins 0.000 description 2
- 102000007651 Macrophage Colony-Stimulating Factor Human genes 0.000 description 2
- 108010046938 Macrophage Colony-Stimulating Factor Proteins 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 108010035766 P-Selectin Proteins 0.000 description 2
- 101000820656 Rattus norvegicus Seminal vesicle secretory protein 4 Proteins 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- 239000012503 blood component Substances 0.000 description 2
- 238000004820 blood count Methods 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 210000002889 endothelial cell Anatomy 0.000 description 2
- 239000012091 fetal bovine serum Substances 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 150000003278 haem Chemical class 0.000 description 2
- 229940072221 immunoglobulins Drugs 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 230000009456 molecular mechanism Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- QZAYGJVTTNCVMB-UHFFFAOYSA-N serotonin Chemical compound C1=C(O)C=C2C(CCN)=CNC2=C1 QZAYGJVTTNCVMB-UHFFFAOYSA-N 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 206010043554 thrombocytopenia Diseases 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- GHCZTIFQWKKGSB-UHFFFAOYSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid;phosphoric acid Chemical compound OP(O)(O)=O.OC(=O)CC(O)(C(O)=O)CC(O)=O GHCZTIFQWKKGSB-UHFFFAOYSA-N 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 102100037529 Coagulation factor V Human genes 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108010014172 Factor V Proteins 0.000 description 1
- 206010017711 Gangrene Diseases 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 102000002268 Hexosaminidases Human genes 0.000 description 1
- 108010000540 Hexosaminidases Proteins 0.000 description 1
- 108090000143 Mouse Proteins Proteins 0.000 description 1
- 108010004729 Phycoerythrin Proteins 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 208000003441 Transfusion reaction Diseases 0.000 description 1
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 1
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 108010083526 asialo-von Willebrand Factor Proteins 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000007321 biological mechanism Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000010836 blood and blood product Substances 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 229940125691 blood product Drugs 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 230000034303 cell budding Effects 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000008614 cellular interaction Effects 0.000 description 1
- 230000033077 cellular process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 230000005757 colony formation Effects 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 210000004292 cytoskeleton Anatomy 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- WDRWZVWLVBXVOI-QTNFYWBSSA-L dipotassium;(2s)-2-aminopentanedioate Chemical compound [K+].[K+].[O-]C(=O)[C@@H](N)CCC([O-])=O WDRWZVWLVBXVOI-QTNFYWBSSA-L 0.000 description 1
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 230000003511 endothelial effect Effects 0.000 description 1
- 210000004954 endothelial membrane Anatomy 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 210000003714 granulocyte Anatomy 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000012744 immunostaining Methods 0.000 description 1
- 210000005061 intracellular organelle Anatomy 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
- 235000013919 monopotassium glutamate Nutrition 0.000 description 1
- 235000013923 monosodium glutamate Nutrition 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000002840 nitric oxide donor Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 210000001778 pluripotent stem cell Anatomy 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 238000002271 resection Methods 0.000 description 1
- 229940076279 serotonin Drugs 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 229940073490 sodium glutamate Drugs 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 239000000021 stimulant Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 230000000472 traumatic effect Effects 0.000 description 1
- 210000001325 yolk sac Anatomy 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0644—Platelets; Megakaryocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/145—Thrombopoietin [TPO]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1394—Bone marrow stromal cells; whole marrow
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Reproductive Health (AREA)
- Developmental Biology & Embryology (AREA)
- Gynecology & Obstetrics (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Diabetes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US62392204P | 2004-11-01 | 2004-11-01 | |
| US60/623,922 | 2004-11-01 | ||
| US71457805P | 2005-09-07 | 2005-09-07 | |
| US60/714,578 | 2005-09-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20070073932A true KR20070073932A (ko) | 2007-07-10 |
Family
ID=36129866
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020077011858A Withdrawn KR20070073932A (ko) | 2004-11-01 | 2005-10-31 | 줄기 세포 유래 혈소판 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20060099198A1 (enExample) |
| EP (1) | EP1807507A2 (enExample) |
| JP (1) | JP2008518603A (enExample) |
| KR (1) | KR20070073932A (enExample) |
| AU (1) | AU2005302258B2 (enExample) |
| CA (1) | CA2586053C (enExample) |
| GB (1) | GB2434157A (enExample) |
| IS (1) | IS8637A (enExample) |
| MX (1) | MX2007005200A (enExample) |
| NZ (1) | NZ554866A (enExample) |
| WO (1) | WO2006050330A2 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8137970B2 (en) | 2008-06-30 | 2012-03-20 | Ewha University-Industry Collaboration Foundation | Methods for inducing the differentiation of hematopoietic stem cells into megakaryocytes and platelets, and gene controlling the differentiation |
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102230288B1 (ko) | 2006-04-14 | 2021-03-22 | 아스텔라스 인스티튜트 포 리제너러티브 메디슨 | 혈관 콜로니 형성 세포 |
| US8058064B2 (en) * | 2006-10-04 | 2011-11-15 | The University Of Tokyo | Sac-like structure enclosing hematopoietic progenitor cells produced from ES cells and method for preparing blood cells |
| WO2008151386A1 (en) * | 2007-06-15 | 2008-12-18 | Australian Stem Cell Centre Ltd | Megakaryocyte differentiation |
| JPWO2009119105A1 (ja) * | 2008-03-28 | 2011-07-21 | 国立大学法人 東京大学 | ＧＰＩｂα＋ＧＰＶ＋ＧＰＶＩ＋血小板のインビトロ調製法 |
| JP5617631B2 (ja) * | 2008-04-01 | 2014-11-05 | 国立大学法人東京大学 | ｉＰＳ細胞からの血小板の調製方法 |
| WO2009137629A2 (en) | 2008-05-06 | 2009-11-12 | Advanced Cell Technology, Inc. | Methods for producing enucleated erythroid cells derived from pluripotent stem cells |
| CN102083963A (zh) | 2008-05-06 | 2011-06-01 | 先进细胞技术公司 | 血管瘤集落形成细胞和非移植血管瘤细胞 |
| JP5863459B2 (ja) * | 2008-12-04 | 2016-02-16 | インセルム （アンスティテュ・ナショナル・ドゥ・ラ・サント・エ・ドゥ・ラ・ルシェルシュ・メディカル）ＩＮＳＥＲＭ （Ｉｎｓｔｉｔｕｔ Ｎａｔｉｏｎａｌ ｄｅ ｌａ Ｓａｎｔｅ ｅｔ ｄｅ ｌａ Ｒｅｃｈｅｒｃｈｅ Ｍｅｄｉｃａｌｅ） | 血小板の産生方法 |
| CA2753208C (en) | 2009-02-20 | 2018-08-21 | Cellular Dynamics International, Inc. | Methods and compositions for the differentiation of stem cells |
| KR101720961B1 (ko) | 2009-02-27 | 2017-03-29 | 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 | 다능성 세포의 분화 |
| CA2782013C (en) | 2009-12-04 | 2021-06-08 | Stem Cell & Regenerative Medicine International, Inc. | Large scale generation of functional megakaryocytes and platelets from human embryonic stem cells under stromal-free conditions |
| EP2625264B1 (en) | 2010-10-08 | 2022-12-07 | Terumo BCT, Inc. | Methods and systems of growing and harvesting cells in a hollow fiber bioreactor system with control conditions |
| WO2012061146A1 (en) | 2010-10-25 | 2012-05-10 | The Children's Hospital Of Philadelphia | Compositions and methods for the generation of platelets and methods of use thereof |
| US8961956B2 (en) | 2011-11-30 | 2015-02-24 | Ocata Therapeutics, Inc. | Mesenchymal stromal cells and uses related thereto |
| HRP20181515T1 (hr) | 2011-11-30 | 2018-11-16 | Astellas Institute For Regenerative Medicine | Mezenhimne stromalne stanice i upotreba povezana s njima |
| WO2014011407A2 (en) | 2012-07-12 | 2014-01-16 | Imstem Biotechnology, Inc. | Mesenchymal-like stem cells derived from human embryonic stem cells, methods and uses thereof |
| CN114558032B (zh) | 2012-12-21 | 2025-07-29 | 安斯泰来再生医药协会 | 由多能干细胞制备血小板的方法及其组合物 |
| EP3068866B1 (en) | 2013-11-16 | 2018-04-25 | Terumo BCT, Inc. | Expanding cells in a bioreactor |
| WO2015148704A1 (en) | 2014-03-25 | 2015-10-01 | Terumo Bct, Inc. | Passive replacement of media |
| JP2015188381A (ja) * | 2014-03-28 | 2015-11-02 | 東レエンジニアリング株式会社 | 分離装置および分離方法 |
| US20160090569A1 (en) | 2014-09-26 | 2016-03-31 | Terumo Bct, Inc. | Scheduled Feed |
| WO2017004592A1 (en) | 2015-07-02 | 2017-01-05 | Terumo Bct, Inc. | Cell growth with mechanical stimuli |
| SG11201802159UA (en) * | 2015-10-14 | 2018-04-27 | Megakaryon Corp | Method for producing purified platelets |
| CA3002157A1 (en) | 2015-10-20 | 2017-04-27 | FUJIFILM Cellular Dynamics, Inc. | Methods for directed differentiation of pluripotent stem cells to immune cells |
| WO2017205667A1 (en) | 2016-05-25 | 2017-11-30 | Terumo Bct, Inc. | Cell expansion |
| US11685883B2 (en) | 2016-06-07 | 2023-06-27 | Terumo Bct, Inc. | Methods and systems for coating a cell growth surface |
| US11104874B2 (en) | 2016-06-07 | 2021-08-31 | Terumo Bct, Inc. | Coating a bioreactor |
| CN110050061A (zh) | 2016-10-05 | 2019-07-23 | 富士胶片细胞动力公司 | 从具有MeCP2破坏的诱导多能干细胞生成成熟谱系 |
| US12234441B2 (en) | 2017-03-31 | 2025-02-25 | Terumo Bct, Inc. | Cell expansion |
| US11624046B2 (en) | 2017-03-31 | 2023-04-11 | Terumo Bct, Inc. | Cell expansion |
| CN110612344B (zh) | 2017-03-31 | 2023-09-12 | 泰尔茂比司特公司 | 细胞扩增 |
| EA201992469A1 (ru) | 2017-04-18 | 2020-05-27 | Фуджифилм Селльюлар Дайнамикс, Инк. | Антигенспецифические иммунные эффекторные клетки |
| JP2021509812A (ja) * | 2018-01-05 | 2021-04-08 | プレートレット バイオジェネシス， インコーポレイテッド | 巨核球を産生するための組成物および方法 |
| WO2020006539A1 (en) | 2018-06-29 | 2020-01-02 | Platelet Biogenesis, Inc. | Compositions for drug delivery and methods of use thereof |
| WO2022204315A1 (en) | 2021-03-23 | 2022-09-29 | Terumo Bct, Inc. | Cell capture and expansion |
| US12209689B2 (en) | 2022-02-28 | 2025-01-28 | Terumo Kabushiki Kaisha | Multiple-tube pinch valve assembly |
| USD1099116S1 (en) | 2022-09-01 | 2025-10-21 | Terumo Bct, Inc. | Display screen or portion thereof with a graphical user interface for displaying cell culture process steps and measurements of an associated bioreactor device |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0934403A4 (en) * | 1996-08-19 | 2001-03-14 | Univ Massachusetts | ROWS OF EMBRYONIC OR STRAIN-LIKE CELLS PRODUCED BY CROSS NUCLEAR TRANSPLANTATION OF SPECIES |
| US6280718B1 (en) * | 1999-11-08 | 2001-08-28 | Wisconsin Alumni Reasearch Foundation | Hematopoietic differentiation of human pluripotent embryonic stem cells |
-
2005
- 2005-10-31 MX MX2007005200A patent/MX2007005200A/es active IP Right Grant
- 2005-10-31 AU AU2005302258A patent/AU2005302258B2/en not_active Expired
- 2005-10-31 JP JP2007539283A patent/JP2008518603A/ja active Pending
- 2005-10-31 CA CA2586053A patent/CA2586053C/en not_active Expired - Lifetime
- 2005-10-31 US US11/262,582 patent/US20060099198A1/en not_active Abandoned
- 2005-10-31 EP EP05824903A patent/EP1807507A2/en not_active Withdrawn
- 2005-10-31 KR KR1020077011858A patent/KR20070073932A/ko not_active Withdrawn
- 2005-10-31 NZ NZ554866A patent/NZ554866A/en not_active IP Right Cessation
- 2005-10-31 WO PCT/US2005/039401 patent/WO2006050330A2/en not_active Ceased
-
2007
- 2007-04-30 IS IS8637A patent/IS8637A/is unknown
- 2007-05-17 GB GB0709533A patent/GB2434157A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8137970B2 (en) | 2008-06-30 | 2012-03-20 | Ewha University-Industry Collaboration Foundation | Methods for inducing the differentiation of hematopoietic stem cells into megakaryocytes and platelets, and gene controlling the differentiation |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2006050330B1 (en) | 2006-10-26 |
| GB0709533D0 (en) | 2007-06-27 |
| CA2586053C (en) | 2013-07-30 |
| GB2434157A (en) | 2007-07-18 |
| NZ554866A (en) | 2010-12-24 |
| JP2008518603A (ja) | 2008-06-05 |
| WO2006050330A3 (en) | 2006-08-24 |
| CA2586053A1 (en) | 2006-05-11 |
| MX2007005200A (es) | 2007-05-11 |
| AU2005302258A1 (en) | 2006-05-11 |
| IS8637A (is) | 2007-04-30 |
| AU2005302258B2 (en) | 2010-10-21 |
| EP1807507A2 (en) | 2007-07-18 |
| US20060099198A1 (en) | 2006-05-11 |
| WO2006050330A2 (en) | 2006-05-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2586053C (en) | Platelets from stem cells | |
| US20200263132A1 (en) | Methods for producing enucleated erythroid cells derived from pluripotent stem cells | |
| JP4146802B2 (ja) | 単球を起源に持つ、脱分化したプログラム可能な幹細胞およびそれらの製造と使用 | |
| US20030175955A1 (en) | Novel embryonic cell populations and methods to isolate such populations | |
| US20080118477A1 (en) | Umbilical cord mesenchymal stem cells support cord blood hematopoiesis | |
| KR20150126943A (ko) | 규정된 조건하에서 인간 만능성 줄기 세포의 조혈내피세포 분화를 위한 방법 및 재료 | |
| JP2016063838A (ja) | 血管コロニー形成細胞 | |
| KR20020013480A (ko) | 사람 뇌의 내피세포 및 성장배지 및 원시 ｃｄ34+ｃｄ38-골수 간세포의 증가방법 | |
| US20070077654A1 (en) | Platelets from stem cells | |
| CN104293731A (zh) | 建鲤原代肝细胞的分离培养方法 | |
| KR20100091192A (ko) | 제대혈 유도된 세포와 월경 줄기 세포의 공동-배양 방법 | |
| KR20040099366A (ko) | 단구성 기원의 탈분화된 프로그램 가능한 줄기 세포, 이의생성 방법 및 용도 | |
| US9670460B2 (en) | Method for using directing cells for specific stem/progenitor cell activation and differentiation | |
| US20080095746A1 (en) | Process For Producing Hematopoietic Stem Cells Or Vascular Endothelial Precursor Cells | |
| CN112126624A (zh) | 一种诱导胚胎干细胞分化生成红细胞的方法 | |
| CN112080469A (zh) | T1肽在体外促进脐带血造血干细胞增殖中的应用 | |
| Lu et al. | Directed differentiation of red blood cells from human embryonic stem cells | |
| CN101052710A (zh) | 来自干细胞的血小板 | |
| KR101318965B1 (ko) | 인공적인 골수-유사 환경을 형성하는 조성물 및 그의 용도 | |
| CN112143697A (zh) | 一种促进胚胎干细胞增殖和分化的方法 | |
| US8956870B2 (en) | Method for using directing cells for specific stem/progenitor cell activation and differentiation | |
| WO2005056778A1 (ja) | 造血幹細胞の分化抑制又は増殖方法 | |
| JPH11180877A (ja) | 巨核球前駆細胞を含む細胞製剤及びその製造方法 | |
| JPH11127859A (ja) | 造血幹細胞の分化・増殖調節方法 | |
| JP2007014229A (ja) | 血液から分離した単核細胞を試験管内で増幅させる方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PA0105 | International application |
Patent event date: 20070525 Patent event code: PA01051R01D Comment text: International Patent Application |
|
| PG1501 | Laying open of application | ||
| PC1203 | Withdrawal of no request for examination | ||
| WITN | Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid |