KR20060125696A - Microbial pesticide composition - Google Patents

Abstract

It is intended to provide a microbial pesticide composition which contains a microorganism having an effect of controlling disease injury on plants, an oxygen-containing inorganic salt and urea and by which the formation of spots on crop surface caused by the application of the microbial pesticide composition is relieved and a sufficient effect of controlling disease injury on plants can be achieved.

Description

Microbial Pesticide Composition {MICROBIAL PESTICIDE COMPOSITION}

BACKGROUND OF THE INVENTION Field of the Invention The present invention relates to a microbial pesticide composition, and more particularly, to a microbial pesticide composition in which about half of the surface of a crop produced by spraying of a microbial pesticide composition is reduced.

In recent years, as a method of protecting plants from various diseases, microbial pesticides using pathogens causing various diseases and microorganisms that antagonize various diseases have been widely used in consideration of safety and sustainability of effects. Usually, as a microbial pesticide, water-insoluble extenders, such as a clay mineral, are used in order to prevent ubiquitous of the active ingredient in a composition, and to improve storage stability. For example, Japanese Patent Application Laid-Open No. 1996-175919 and Japanese Patent Application Laid-Open No. 1996-175920 disclose microbial pesticides using talc as an extender. However, in the case of a microbial pesticide composition containing a large amount of insoluble minerals such as talc as an extender, when the microbial pesticide composition is sprayed, the fine powder of the extender in the composition adheres to the surface of the crop, causing crops to appear in the crop. There was a problem of lowering.

In order to prevent such medicine at the time of spraying a pesticide, the method of removing an insoluble extender from a microbial pesticide composition is considered, but when it does so, when the microorganism which is an active ingredient is ubiquitous, a composition will become uneven and it will melt | dissolve in a spreading liquid There are drawbacks such as deterioration in the dispersibility of the microbial pesticide composition. On the other hand, it is also possible to change the extender to be water-soluble, but depending on the type of extender, there has been a problem such that the effect of the microbial pesticide composition is lowered or the storage stability of the microbial pesticide composition is lowered.

Therefore, about half of the crop surface caused by the spraying of the microbial pesticide composition is reduced, and a microbial pesticide composition having a sufficient plant disease control effect has been demanded.

Disclosure of the Invention

The present invention has been made in view of the above, and an object of the present invention is to provide a microbial pesticide composition having less than about half of the surface of a crop produced by spraying of a microbial pesticide composition and having a sufficient plant disease control effect.

MEANS TO SOLVE THE PROBLEM As a result of earnestly examining in order to solve the said subject, the present inventors discovered that the said objective can be achieved by using the microorganism, oxygen-containing inorganic salt, and urea which have the effect of controlling a plant disease. In accordance with these findings, the present invention has been completed.

That is, the summary of this invention is as follows.

(1) A microbial pesticide composition containing microorganisms, oxygen-containing inorganic salts, and urea having an effect of controlling plant diseases.

(2) The content of the oxygen-containing inorganic salt is 10 to 50% by weight based on the total amount of the microorganism, the oxygen-containing inorganic salt and the urea, and the content of the urea is from the microorganism, the oxygen-containing inorganic salt and the urea. It is 10-50 weight% with respect to the total amount, The microorganism pesticide composition as described in (1) characterized by the above-mentioned.

(3) The microorganism pesticide composition according to (1) or (2), wherein the content of the microorganism is 10 to 30% by weight based on the total amount of the microorganism, the oxygen-containing inorganic salt and the urea.

(4) A microbial pesticide composition containing microorganisms, oxygen-containing inorganic salts, urea and surfactants having a plant disease control effect.

(5) The content of the oxygen-containing inorganic salt is from 10 to 50% by weight based on the total amount of the microorganism, the oxygen-containing inorganic salt and the urea, and the content of the urea is from the microorganism, the oxygen-containing inorganic salt and the urea. It is 10-50 weight% with respect to the total amount, The content of the said surfactant is 5-30 weight% with respect to the said microorganism pesticide composition whole, The microorganism pesticide composition as described in (4) characterized by the above-mentioned.

(6) The microorganism pesticide composition according to (4) or (5), wherein the content of the microorganism is 10 to 30% by weight based on the total amount of the microorganism, the oxygen-containing inorganic salt and the urea.

(7) The microbial pesticide composition according to any one of (4) to (6), wherein the surfactant is an anionic type.

(8) The microbial pesticide composition according to any one of (1) to (7), wherein the oxygen-containing inorganic salt is a carbonate or sulfate.

(9) The microbial pesticide composition according to (8), wherein the carbonate is sodium hydrogen carbonate.

(10) The microbial pesticide composition according to (8) or (9), wherein the sulfate is sodium sulfate.

(11) The microbial pesticide composition according to any one of (1) to (10), wherein the microorganism is a Bacillus bacterium.

(12) The microbial pesticide composition according to (11), wherein the genus Bacillus bacterium is Bacillus subtilis.

(13) The microorganism pesticide composition according to (11), wherein the genus Bacillus bacteria is NCIB12376 strain or NCIB12616 strain.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

The microbial pesticide composition of the present invention is a microbial pesticide composition containing microorganisms, oxygen-containing inorganic salts and urea having a plant disease control effect.

The microorganism used in the present invention is not particularly limited as long as it is a microorganism having an effect of controlling plant diseases. In the present invention, the "microorganism having the effect of controlling plant diseases" is the ability to prevent the occurrence of any plant diseases or to cure plant diseases by treating the plants with the microorganisms, such as spraying the microorganisms on the plants. It refers to a microorganism having a.

Such microorganisms include Bacillus bacteria, gliocladium bacteria, trichoderma bacteria, taralomyces bacteria, penicillin bacteria and the like. Bacillus bacteria, for example, is effective in the control of gray fungus, powdery mildew (Powdery mildew) and soot fungus disease, and bacteria in the gliocladium, effective in the control of gray mold, etc. , Trichoderma bacteria are effective in controlling gray fungal diseases, Taralomyces bacteria are effective in controlling powdery mildew and anthrax, and penicillin bacteria are effective in controlling anthrax and the like. . Since it exhibits a control effect against various plant diseases, as a microorganism used for this invention, the genus Bacillus bacterium is preferable and Bacillus subtilis is more preferable. As such Bacillus subtilis, NCIB12376 strain and NCIB12616 strain are mentioned, for example. NCIB12376 strains and NCIB12616 strains are available from National Collections of Industrial, Food and Marine Bacteria (NCIMB).

The microorganism used for this invention can be used individually by 1 type, or can use multiple types simultaneously.

As a microorganism used for this invention, what is contained in commercially available probiotics etc. can be used, for example, and what was cultured using the commercial strain can be used.

The method of culturing the microorganism used for this invention is not specifically limited, It can be set as a regular method. For example, microorganisms may be cultured in a proliferative medium, and the cells may be recovered by means of centrifugation or the like.

The microorganisms used in the present invention are live microorganisms. In addition, although the live microbe of the microorganism used for this invention may not be a spore, it is preferable that it is a spore from a viewpoint of the preservation | save as a product of a microorganism pesticide composition. Therefore, in order to form spores, it is prepared to make culture conditions, such as a composition of a medium, pH of a medium, culture temperature, culture humidity, and oxygen concentration at the time of cultivation, suitable for the spore formation conditions at the end of culture. It is desirable to. In the present invention, when using spores of microorganisms, it is preferable to set the water content of spores to 20% by weight or less from the viewpoint of preservation of the microorganism pesticide composition.

The amount of microorganisms contained in the microbial pesticide composition of the present invention is not particularly limited as long as the effects of the present invention are not impaired. However, the amount of microorganisms, oxygen-containing inorganic salts and urea may be 5 to 50% by weight ( Dry weight), and more preferably 10 to 30% by weight. The concentration of microorganisms contained in the microbial pesticide composition of the present invention is preferably 1 × 10 ⁵ to 1 × 10 ¹⁵ cfu / g, more preferably 1 × 10 ⁹ to 1 × 10 as the colony forming unit. Can be ¹² cfu / g.

The oxygen-containing inorganic salt used in the present invention is not particularly limited as long as it does not interfere with the effects of the present invention. Examples thereof include carbonates, sulfates, phosphates and nitrates, but carbonates and sulfates are preferable. Sodium is also particularly preferred as sulfate.

The amount of the oxygen-containing inorganic salt included in the microbial pesticide composition of the present invention is not particularly limited as long as the effects of the present invention are not impaired, but it is preferably 10 to 50% by weight based on the total amount of the microorganism, the oxygen-containing inorganic salt and urea. It is more preferable to set it as 20 to 45 weight%. When the oxygen-containing inorganic salt is contained in these ranges, a well-balanced microbial pesticide from the viewpoint of controlling the plant diseases, the hydration of the microbial pesticide composition, and the reduction of the drug surface of the crop surface caused by the spraying of the microbial pesticide composition. This is because a composition is obtained.

The amount of urea contained in the microbial pesticide composition of the present invention is not particularly limited as long as the effect of the present invention is not impaired, but it is preferably 10 to 50% by weight based on the total amount of microorganisms, oxygen-containing inorganic salts and urea, It is more preferable to set it as 20 to 45 weight%. When urea is contained in these ranges, a well-balanced microbial pesticide composition is obtained in view of the control effect against plant diseases, the hydration of the microbial pesticide composition, and the reduction of the rough surface of the crop surface caused by the spraying of the microbial pesticide composition. Because it is obtained.

In addition, although the microorganism pesticide composition of this invention does not need to contain surfactant, it is preferable to include it. It is because dispersibility and suspension property in the sparging liquid of the microorganism which is an active ingredient improve when a microbial pesticide composition is diluted and prepared when a surfactant is contained when surfactant is contained. There is no restriction | limiting in particular as surfactant used for this invention, unless it interferes with the effect of this invention, Any type of surfactant, such as an anion type and a cationic type, may be sufficient, but an anionic type, such as SOLPOL5082 (made by Tokyo Chemical Co., Ltd.). Surfactant is preferred.

The amount of the surfactant contained in the microbial pesticide composition of the present invention is not particularly limited as long as the effect of the present invention is not impaired. However, the amount of the surfactant is preferably 5 to 30% by weight based on the total amount of the microbial pesticide composition, and 8 to 20% by weight. It is more preferable to set it as. This is because when the surfactant is contained in these ranges, the hydration and suspension properties of the microorganisms are improved, the preservation of the microorganism pesticide composition is sufficiently secured, and the damage to plants due to the spraying of the microorganism pesticide composition hardly occurs. .

In addition, the microbial pesticide composition of the present invention may contain, in addition to the above-mentioned substances, as long as it does not interfere with the effects of the present invention, such as a water-soluble substance.

There is no restriction | limiting in particular as a formulation of the microorganism pesticide composition of this invention, Although granular form, powder form, liquid phase, etc. may be sufficient, granular form and powder form are preferable. It is preferable to exist in the range of 1 micrometer-100 micrometers, and, as for the average particle diameter in that case, it is more preferable to exist in the range which is 1 micrometer-50 micrometers. If the average particle diameter is within such a range, the microbial pesticide composition can be easily handled, and if the particle diameter is 1 μm or less, the spore diameter of the microorganism may be lowered, so that the microorganisms die and control against plant diseases. This is because the effect may not be sufficiently exhibited.

The microbial pesticide composition of this invention can be used for control of plant diseases, such as eggplant gray mold disease and petal gray mold disease.

The method for using the microbial pesticide composition of the present invention is not particularly limited. For example, 1000 times, 2000 times, 4000 times, etc., the spraying solution is prepared by diluting with water so as to have a predetermined dilution rate determined at the time of pesticide registration. The liquid can be used by spraying the entire plant into a fog shape using a power sprayer or the like.

Hereinafter, the present invention will be described in more detail with reference to Examples.

<Example 1>

Production Example 1 Preparation of Wet Cells (Spores) of Bacillus Bacteria

Three 500 ml volumetric flasks containing 100 ml of Buillon medium (1% by weight of meat extract, 1% by weight of peptone, 0.5% by weight of sodium chloride) were prepared, respectively. Slope cultures of preservative bacteria of subtilis NCIB12376 strains were inoculated by 1 platinum, and then incubated at 30 ° C for 1 day while shaking the flask at an amplitude of 10 cm and a rotation speed of 120 rpm using a reciprocating vibrator. .

Meanwhile, a 30 L volume fermenter containing 15 L of medium (2 wt% glucose, 1 wt% polypeptone, 0.2 wt% yeast extract, 0.1 wt% KH ₂ PO ₄ ) was prepared to prepare a total of 300 ml of the culture obtained in advance. Was cultured in the fermenter and incubated at 30 ° C. for 72 hours under aerobic conditions to obtain a culture solution. About 15 L of the obtained culture was centrifuged (6000 rpm, 20 minutes) according to a regular method, and the culture supernatant and the cell precipitate were separated. After removing the culture supernatant, the cell precipitate was washed with water to give a wet weight of about 780 g of wet cells (spore fraction). This spore fraction contains 100% by weight of spores of Bacillus subtilis NCIB12376 strain in a dried state.

(Manufacture example 2)

Instead of using the Bacillus subtilis NCIB12376 strain, the same procedure as in Production Example 1 was performed using the Bacillus subtilis NCIB12616 strain to obtain a spore fraction having a wet weight of about 750 g. This spore fraction contains 50% by weight of spores of Bacillus subtilis NCIB12616 strain in a dry state.

Production Example 3 Preparation of Powder Spores

Approximately 600 g of the spore fraction of Bacillus subtilis NCIB12376 strain obtained in Preparation Example 1 was suspended in 300 L of water, and then treated with a spray dryer at a rate of 1.5 to 2.0 L / h (inlet temperature 150 ° C., outlet). Temperature 100 ° C.). The dry matter obtained by the spray drier was pulverized to obtain spore powder having a dry weight of about 78 g.

(Manufacture example 4)

About 600 g of the spore fraction obtained in Production Example 2 was treated in the same manner as in Production Example 3 to obtain about 78 g of spore powder of Bacillus subtilis NCIB12616 strain.

(Manufacture example 5)

Using spore powder of Bacillus subtilis NClB12376 strain obtained in Preparation Example 3 above, the materials shown in Table 1 were mixed in the composition shown in Table 1, and finely pulverized using a jet mill grinder, followed by ribbon blender. By stirring and mixing, Formulation Examples 1, 2 and 3, and Comparative Examples 1, 2, 3, 4 and 5 were obtained.

Further, using the spore powder of Bacillus subtilis NCIB12616 strain obtained in Production Example 4 above, the compounds shown in Table 1 were mixed in the composition shown in Table 1, and then pulverized using a jet mill grinder, followed by a ribbon blender. The mixture was stirred and mixed well to obtain Formulation Example 4.

In addition, SOLPOL5082 (made by Tokyo Chemical Co., Ltd.) which is an anionic surfactant was used as surfactant.

In order to investigate the storage stability of the microbial pesticide composition prepared in Production Example, the number of bacteria in the composition was measured over time. On the other hand, the composition described in Example 1 of Unexamined-Japanese-Patent No. 1996-175920 was produced, and this was made into the comparative example 6, and the bacterial count in the composition was similarly measured over time. The results are shown in Table 2.

As is apparent from Table 2, the microbial pesticide composition of the present invention is superior in storage stability as compared with the comparative example.

<Example 3>

Since the pesticide composition of this invention sprays and uses what diluted with water, it investigated whether the microorganism pesticide composition of this invention is sufficient in hydration property. Hydration was measured as follows.

0.3077 g of calcium carbonate and 0.092 g of magnesium carbonate were dissolved in a small amount of 1N dilute hydrochloric acid. The resulting solution was evaporated to dryness on a sand bath. The remaining solid content was dissolved in 10 L of demineralized water to obtain test water for the water solubility test. Next, a 500 ml volume beaker containing 200 ml of the test water was prepared, and 5 g of the pesticide composition of the present invention was sprayed to spread throughout the surface from a position of about 10 cm on the water surface in the beaker. After spraying an agrochemical composition on the water surface, the time until it sank to the water bottom was measured, and it was set as the index of hydration.

Table 3 shows the results of this hydration test.

<Example 4>

It was examined whether the microbial pesticide composition of the present invention exerts sufficient control effect against plant diseases.

(1) Eggplant gray mold control test

The control effect against eggplant gray mold disease was tested in a vinyl greenhouse (breed: Cheonyang No. 2, 5 weeks / gu, 3 repetitions). The inoculation of eggplant gray mold disease was carried out by attaching eggplant fruit which was infected by cultivating the fungus of gray fungus in a greenhouse. In addition, the microbial agrochemical composition, Formulation Example 1 (2000-fold dilution), Formulation Example 2 (1000-fold dilution), Formulation Example 3 (1000-fold dilution), Formulation Example 4 (2000-fold dilution) diluted with water in the flowering and deletion of the eggplant Dilution), Comparative Example 1 (1000-fold dilution), and Comparative Example 6 (1000-fold dilution) were sprayed every 7 days. Spraying was carried out by spraying 300 L per 10 a dilution of each pesticide composition throughout the week. Seven days after the third spray, each strain was irradiated to calculate the onset fruiting rate. The results are shown in Table 4.

In addition, the same test was carried out without spraying a formulation example and a comparative example, and the onset excess ratio was computed, and the control value of the formulation example and the comparative example was computed based on this numerical value.

(2) petal gray mold control test

Petunia petals were used to investigate the effects of Formulations and Comparative Examples on Gray Mold Disease. Inoculation of gray mold disease was performed by spraying petals with the aqueous solution containing 1x10 <^5> cfu / g pathogen of gray mold disease. Such petals were treated by spraying Formulation Example 1 (2000-fold dilution), Comparative Example 1 (1000-fold dilution), Comparative Example 6 (1000-fold dilution) diluted with water, and after 1 day at 20 ° C, The shape of the petals of petunia was investigated. The results are shown in Table 5.

From the results of Table 5, it was confirmed that the microbial pesticide composition of the present invention has a sufficient control effect against the disease of the plant.

Example 5

In the microbial pesticide composition of the present invention, it was examined whether or not about half of the crop surface caused by the spraying of the microbial pesticide composition was actually reduced.

The 1000-fold dilution liquid of Formulation Example 2, the 1000-fold dilution liquid of Comparative Example 6, and the eggplant (kind: Cheonyang No. 2) were prepared and subjected to the same treatment as in Example 4 (1), and then the appearance of the eggplant was examined. The results are shown in Table 6.

-: No contamination by medicine.

+: Slight contamination by the medicine was found.

++: Clear contamination by drugstore was identified.

From the results of Table 6, it can be seen that in the microbial pesticide composition of the present invention, about half of the crop surface caused by the spraying of the microbial pesticide composition is actually reduced.

Advantageous Effects of Invention The present invention provides a microbial pesticide composition which reduces the half of the surface of the crop resulting from the spraying of the microbial pesticide composition, and has a sufficient plant disease control effect.

Claims (13)

A microbial pesticide composition containing microorganisms, oxygen-containing inorganic salts and urea having the effect of controlling plant diseases. The method of claim 1, The content of the oxygen-containing inorganic salt is 10 to 50% by weight based on the total amount of the microorganism, the oxygen-containing inorganic salt and the urea, and the content of the urea is the total amount of the microorganism, the oxygen-containing inorganic salt and the urea. Microbial pesticide composition, characterized in that 10 to 50% by weight. The method according to claim 1 or 2, The microorganism pesticide composition, characterized in that the content of the microorganism is 10 to 30% by weight based on the total amount of the microorganism, the oxygen-containing inorganic salt and the urea. A microbial pesticide composition containing microorganisms, oxygen-containing inorganic salts, urea and surfactants having a plant disease control effect. The method of claim 4, wherein The content of the oxygen-containing inorganic salt is 10 to 50% by weight based on the total amount of the microorganism, the oxygen-containing inorganic salt and the urea, and the content of the urea is the total amount of the microorganism, the oxygen-containing inorganic salt and the urea. It is 10 to 50% by weight, and the content of the surfactant is 5 to 30% by weight based on the entire microbial pesticide composition. The method according to claim 4 or 5, The microorganism pesticide composition, characterized in that the content of the microorganism is 10 to 30% by weight based on the total amount of the microorganism, the oxygen-containing inorganic salt and the urea. The method according to any one of claims 4 to 6, Microbial pesticide composition, characterized in that the surfactant is anionic. The method according to any one of claims 1 to 7, Microbial pesticide composition, characterized in that the oxygen-containing inorganic salt is a carbonate or sulfate. The method of claim 8, Microbial pesticide composition, characterized in that the carbonate is sodium bicarbonate. The method according to claim 8 or 9, Microbial pesticide composition, characterized in that the sulfate is sodium sulfate. The method according to any one of claims 1 to 10, The microorganism pesticide composition, characterized in that the microorganism is Bacillus (bacillus) bacteria. The method of claim 11, The bacterium of the genus Bacillus is a microbial pesticide composition, characterized in that Bacillus subtilis (bacillus subtilis). The method of claim 11, The bacterium genus microorganism pesticide composition, characterized in that the NCIB12376 strain or NCIB12616 strain.