WO2005023004A1

WO2005023004A1 - Microbial pesticide composition

Info

Publication number: WO2005023004A1
Application number: PCT/JP2004/011532
Authority: WO
Inventors: Akihiko Narutaki
Original assignee: Idemitsu Kosan Co., Ltd.
Priority date: 2003-09-03
Filing date: 2004-08-11
Publication date: 2005-03-17
Also published as: CN1845677A; JP2005075813A; TW200511932A; KR20060125696A

Abstract

It is intended to provide a microbial pesticide composition which contains a microorganism having an effect of controlling disease injury on plants, an oxygen-containing inorganic salt and urea and by which the formation of spots on crop surface caused by the application of the microbial pesticide composition is relieved and a sufficient effect of controlling disease injury on plants can be achieved.

Description

Specification

Microbial pesticide composition

Technical field

The present invention relates to a microbial pesticide composition, and more particularly, to a microbial pesticide composition in which drug spots on a crop surface caused by spraying the microbial pesticide composition are reduced.

Background art

[0002] In recent years, as a method of protecting plants from various diseases, microbial pesticides using microorganisms that antagonize pathogenic bacteria causing various diseases have been widely used in consideration of safety and sustainability of the effects. In general, a microbial pesticide uses a water-insoluble extender, such as a clay mineral, in order to prevent uneven distribution of an active ingredient in a composition and to improve storage stability. For example, Patent Documents 1 and 2 disclose microbial pesticides using talc as a bulking agent. However, in the case of a microbial pesticide composition containing a large amount of insoluble minerals such as talc as a bulking agent, when the microbial pesticide composition is sprayed, the fine powder of the bulking agent in the composition adheres to the surface of the crop and the crop is treated with a drug. There was the problem of causing spots and reducing the commercial value of the crop.

In order to prevent such spots when spraying pesticides, it is conceivable to remove insoluble bulking agents from the microbial pesticide composition.However, in such a case, the active ingredient microorganisms are unevenly distributed. However, there are drawbacks such as non-uniformity and poor dispersibility of the microbial pesticide composition when dissolved in the spray liquid. On the other hand, it is conceivable to change the extender to a water-soluble one. was there.

Therefore, there has been a demand for a microbial pesticide composition which reduces the spots on the crop surface caused by the application of the microbial pesticide composition and has a sufficient plant disease control effect.

Patent Document 1: JP-A-8-175919

Patent Document 2: JP-A-8-175920

Disclosure of the invention

[0003] The present invention has been made in view of the above, and has been developed by spraying a microbial pesticide composition. It is an object of the present invention to provide a microbial pesticide composition in which drug spots on the surface of a product are reduced and which have a sufficient plant disease control effect.

The present inventors have conducted intensive studies to solve the above-mentioned problems, and as a result, have achieved the above object by using a microorganism having an effect of controlling plant diseases, an oxygen-containing inorganic salt, and urea. The inventors have found that the present invention has been completed, and based on these findings, have completed the present invention.

That is, the gist of the present invention is as follows.

(1) A microbial pesticide composition containing a microorganism having an effect of controlling plant diseases, an oxygen-containing inorganic salt, and urea.

(2) The content of the oxygen-containing inorganic salt is 10 to 50% by weight based on the total amount of the microorganism, the oxygen-containing inorganic salt and the urine, and the content of the urea is The microbial pesticide composition according to (1), wherein the amount is 10 to 50% by weight based on the total amount of the microorganism, the oxygen-containing inorganic salt and the urea.

(3) The content of (1) or (2), wherein the content of the microorganism is 10 to 30% by weight based on the total weight of the microorganism, the oxygen-containing inorganic salt, and the urea. Microbial pesticide composition.

(4) A microbial pesticide composition comprising a microorganism having a plant disease controlling effect, an oxygen-containing inorganic salt, urea, and a surfactant.

(5) The content of the oxygen-containing inorganic salt is 10 to 50% by weight based on the total amount of the microorganism, the oxygen-containing inorganic salt and the urine, and the content of the urea is The total amount of the oxygen-containing inorganic salt and the urea is 10 to 50% by weight, and the content of the surfactant is 530% by weight based on the whole microbial pesticide composition. The microbial pesticide composition according to (4).

(6) The micro-organism according to (4) or (5), wherein the content of the microorganism is 1030% by weight based on the total weight of the microorganism, the oxygen-containing inorganic salt, and the urea. Biopesticide composition.

(7) The microbial pesticide composition according to (1), wherein the surfactant is an anion type. (8) The microbial pesticide composition according to any one of (1) to (7), wherein the oxygen-containing inorganic salt is a carbonate or a sulfate.

(9) The microbial pesticide composition according to (8), wherein the carbonate is sodium bicarbonate.

(10) The microbial pesticide composition according to (8) or (9), wherein the sulfate is sodium sulfate.

(11) The microbial pesticide composition according to any one of (1) to (10), wherein the microorganism is a Bacillus bacterium.

(12) The microbial pesticide composition according to (11), wherein the Bacillus bacterium is Bacillus subtilis.

(13) The microbial pesticide composition according to (11), wherein the Bacillus bacterium is NCIB12376 strain or NCIB12616 strain.

BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, the present invention will be described in detail.

The microbial pesticide composition of the present invention is a microbial pesticide composition containing a microorganism having a plant disease controlling effect, an oxygen-containing inorganic salt and urea.

The microorganism used in the present invention is not particularly limited as long as it is a microorganism having an effect of controlling plant diseases. The term "microorganism having an effect of controlling plant diseases" in the present invention refers to preventing the occurrence of a certain plant disease or treating a plant disease by treating the plant with the microorganism, such as spraying the microorganism on the plant. A microorganism that has the ability to heal bacteria.

Examples of such microorganisms include Bacillus bacteria, Dariocladium bacteria, Trichoderma bacteria, Talalomyces bacteria, Penicillium bacteria and the like. Bacillus bacteria are effective in controlling, for example, gray mold, powdery mildew, and mildew, Daliocladium bacteria are effective in controlling gray mold, etc., and Trichoderma bacteria are effective in controlling gray mold. It is effective in controlling disease and the like, Talalomyces bacteria are effective in controlling powdery mildew and anthracnose, and Penicillium bacteria are effective in controlling anthracnose and the like. Since it exerts a controlling effect on various plant diseases, it can be used as a microorganism in the present invention. Is more preferably Bacillus subtilis, which is preferred by Bacillus bacteria. Such Bacillus subtilis includes, for example, strains NCIB12376 and NCIB12616.

The NCIB12376 and NCIB12616 strains are available from the National Collections of Industrial, Food and Marine Bacteria (NCIMB).

The microorganism used in the present invention may be used alone or in combination of two or more.

As the microorganism used in the present invention, for example, a microorganism contained in a commercially available viable agent or the like, or a microorganism cultured using a commercially available bacterial strain may be used.

The method for culturing the microorganism used in the present invention is not particularly limited, and can be performed by a conventional method. For example, the cells can be cultured in a medium in which microorganisms can grow, and the cells can be collected by means such as centrifugation.

The microorganism used in the present invention is a live microorganism. The microorganisms used in the present invention may not be spores, but are preferably spores from the viewpoint of preservability of the microbial pesticide composition as a product. Therefore, in order to form spores, at the end of the culture, the culture conditions such as the composition of the medium, the pH of the medium, the culture temperature, the culture humidity, and the oxygen concentration during culturing are adjusted to match the spore formation conditions. Is preferred. When spores of microorganisms are used in the present invention, the water content of the spores is preferably 20% by weight or less from the viewpoint of the preservability of the microbial pesticide composition.

The amount of microorganisms contained in the microbial pesticide composition of the present invention is not particularly limited as long as the effects of the present invention are not impaired, but is 5 to 50% based on the total amount of microorganisms, oxygen-containing inorganic salts and urea. %, Preferably from 10 to 30% by weight (dry weight). The concentration of microorganisms contained in the microbial pesticide composition of the present invention is preferably 1 × 10 ⁵ 1 × 10 ¹⁵ cfti / g, more preferably 1 × 10 ⁹ —1 × 10 ¹² It can be cfu / g.

The oxygen-containing inorganic salt used in the present invention is not particularly limited as long as the effects of the present invention are not hindered.Examples include carbonates, sulfates, phosphates, and nitrates. Sodium hydrogen carbonate is particularly preferred as a carbonate, and sodium sulfate is particularly preferred as a sulfate. The amount of the oxygen-containing inorganic salt contained in the microbial pesticide composition of the present invention is not particularly limited as long as the effects of the present invention are not impaired. Preferably it is 50% by weight, more preferably 20-45% by weight. When the oxygen-containing inorganic salt is contained in these ranges, from the viewpoint of controlling effect on plant diseases, hydration of the microbial pesticide composition, and reduction of spots on the crop surface caused by spraying the microbial pesticide composition, This is because a microbial pesticide composition with a good balance can be obtained.

The amount of urea contained in the microbial pesticide composition of the present invention is not particularly limited as long as the effects of the present invention are not impaired, but is 1050% by weight based on the total amount of microorganisms, oxygen-containing inorganic salts and urea. 20% to 45% by weight is more preferable. When urea is contained in these ranges, a well-balanced microorganism can be obtained from the viewpoint of controlling plant diseases, hydrating the microbial pesticide composition, and reducing the spots on the crop surface caused by the application of the microbial pesticide composition. This is because an agricultural chemical composition can be obtained.

The microbial pesticide composition of the present invention may not contain a surfactant, but preferably contains a surfactant. This is because when a surfactant is contained, the dispersibility and suspension of the microorganism, which is an active ingredient, in the spray liquid are improved when the spray liquid is prepared by diluting the microbial pesticide composition. The surfactant used in the present invention may be any type of surfactant, such as an anion type and a cationic type, which is not particularly limited, as long as the effects of the present invention are not hindered, but SOLPOL5082 ( Anionic surfactants such as Toho Chemical Co., Ltd.) are preferred.

The amount of the surfactant to be contained in the microbial pesticide composition of the present invention is not particularly limited as long as the effects of the present invention are not impaired, but it is preferably 5 to 30% by weight based on the total amount of the microbial pesticide composition. Preferably, the content is more preferably 8 to 20% by weight. When the surfactant is contained in these ranges, the hydration property of the microorganisms is improved, the susceptibility of the microorganisms is improved, and the preservation of the microbial pesticide composition is sufficiently ensured. This is because almost no phytotoxicity occurs.

In addition, the microbial pesticide composition of the present invention may contain, in addition to the above-mentioned substances, a water-soluble substance, for example, as long as it does not hinder the effects of the present invention.

The dosage form of the microbial pesticide composition of the present invention is not particularly limited, and may be granular, powdery, or liquid. Etc., but are preferably in the form of granules or powder. In this case, the average particle diameter is more preferably in the range of 1 μΐη-100 μΐη, and more preferably in the range of 1 μΐη-50 / im. When the average particle size is within such a range, the microbial pesticide composition is easy to handle, and when the average particle size is lxm or less, the microbial spore diameter may be smaller than the microbial spore diameter. This is because there is a case where the plant is killed and the effect of controlling plant diseases is not sufficiently exhibited.

The microbial pesticide composition of the present invention can be used for controlling plant diseases such as gray mold and petal gray mold.

The method for using the microbial pesticide composition of the present invention is not particularly limited, but may be diluted with water so as to have a predetermined dilution ratio determined at the time of pesticide registration, for example, 1,000 times, 2000 times, 4000 times, and the like. A spray liquid can be prepared, and the spray liquid can be used, for example, by spraying the whole plant in a mist state using a power sprayer or the like.

Hereinafter, the present invention will be described more specifically with reference to examples.

Example

(Production Example 1) Production of wet cells (spore fraction) of Bacillus bacteria

Bouillon medium of 100 ml (meat extract 1% by weight, peptone 1%, sodium chloride 0.5 by weight ^_0/0) are prepared three Sakaguchi flask 500ml flasks containing, respectively, Bacillus' Zubuchi squirrel NCIB12376 strain After inoculating one platinum loop of the slant culture of the stock bacterium, the flask was cultured at 30 ° C. for 1 day while shaking the flask at 10 cm amplitude and 120 rpm using a reciprocating shaker.

On the other hand, a 30-liter fermenter containing a 15-liter medium (glucose 2% by weight, polypeptone 1% by weight, yeast extract 0.2% i%, KHPO 0.1% by weight) was prepared, and the culture obtained above was prepared.

twenty four

A total of 300 ml of the product was inoculated into the fermenter and cultured under aerobic conditions at 30 ° C. for 72 hours to obtain a culture solution. About 15 L of the obtained culture solution was centrifuged (6000 rpm, 20 minutes) according to a standard method to separate into a culture supernatant and a cell precipitate. After removing the culture supernatant, the cell precipitate was washed with water to obtain a wet cell (spore fraction) with a wet weight of about 780 g. This spore fraction, and have you in a dry state, are those containing 100 parts by weight ^_0/0 spores of Bacillus' subtilis NCIB12376 shares (Production Example 2)

Instead of using Bacillus subtilis NCIB12376, the same operation as in Production Example 1 was performed using Bacillus subtilis NCIB12616 to obtain a spore fraction having a wet weight of about 750 g. This spore fraction contains 50% by weight of spores of Bacillus subtilis NCIB 12616 in a dry state.

(Production Example 3) Production of powdered spores

About 600 g of the spore fraction of Bacillus subtilis NCIB12376 strain obtained in Production Example 1 above was suspended in 300 L of water, and then treated with a spray dryer at 1.5-2.OL / h (inlet temperature 150 C, outlet temperature 100 ° C). The dried product obtained by the spray dryer was pulverized to obtain a spore powder having a dry weight of about 78 g.

(Production Example 4)

About 600 g of the spore fraction obtained in Production Example 2 was treated in the same manner as in Production Example 3 to obtain about 78 g of a spore powder of Bacillus subtilis NCIB12616 strain.

(Production Example 5)

Using the spore powder of Bacillus' subtilis NCIB12376 strain obtained in Production Example 3 above, the substances described in Table 1 were mixed with the composition shown in Table 1, and then finely pulverized using a jet mill. The mixture was thoroughly stirred and mixed with a ribbon blender to obtain Formulation Examples 1, 2, and 3, and Comparative Examples 1, 2, 3, 4, and 5.

Using the spore powder of Bacillus subtilis NCIB12616 strain obtained in Production Example 4 above, the compounds shown in Table 1 were mixed with the composition shown in Table 1, and finely pulverized using a jet mill. After that, the mixture was thoroughly stirred and mixed with a ribbon blender to obtain Formulation Example 4.The surfactant was 3〇1〇-〇5082 (manufactured by Toho Kagaku Co., Ltd.) using an anion-type surfactant. Was used.

[table 1]

In order to examine the storage stability of the microbial pesticide composition produced in Production Examples, the number of bacteria in the composition was measured over time. The composition described in Example 1 of JP-A-8-175920 was prepared, and this was used as Comparative Example 6, and the number of bacteria in the composition was similarly measured over time. Table 2 shows the results.

[Table 2]

As is clear from Table 2, the microbial pesticide composition of the present invention is more excellent in qualities than the comparative examples. <Example 3>

Since the agricultural chemical composition of the present invention was diluted with water and used by spraying, it was examined whether or not the microbial agricultural chemical composition of the present invention was sufficiently hydrated. Hydration was measured as follows. 0.3077 g of calcium carbonate and 0.092 g of magnesium carbonate were dissolved in a small amount of 1N diluted hydrochloric acid. The resulting solution was evaporated to dryness on a sand bath. The remaining solid was dissolved in 10 L of demineralized water to make test water for the water solubility test. Next, a 500 ml beaker containing 200 ml of the test water was prepared, and a positioning force of about 10 cm above the water surface in the beaker was sprayed so that 5 g of the pesticidal composition of the present invention was spread over the whole water surface. The time from when the pesticide composition was sprayed on the water surface until it sank to the water floor was measured and used as an index of hydration.

Table 3 shows the results of this hydration test.

[Table 3] Table 3

Example 4>

It was examined whether the microbial pesticide composition of the present invention exerts a sufficient control effect on plant diseases.

(1) Eggplant mildew control test

The control effect on the eggplant gray mold was tested in a vinyl greenhouse (variety: Senryo No. 2, 5 test plots / division, 3 replicates). Inoculation of eggplant gray mold was carried out by hanging eggplant fruits cultivated by infection with gray mold and hanging them in a greenhouse. In addition, the microbial pesticide composition was formulated in the form of eggplant flowering, fruiting season, diluted with water in Formulation Example 1 (2000-fold dilution), Formulation Example 2 (1000-fold dilution), Formulation Example 3 (1000-fold dilution), Formulation Example 4 (2000-fold dilution), Comparative Example 1 (1000-fold dilution), and Comparative Example 6 (1000-fold dilution) were each sprayed at 7-day intervals. Spraying was performed by spraying a diluted solution of each pesticide composition on the entire plant at a rate of 300 L per 10a. Seven days after the third application, each strain was examined, and the diseased flower rate was calculated. The results are shown in Table 4.In addition, the same test was conducted without spraying the formulation example and the comparative example, and the flowering rate of the diseased fruit was calculated, and the control value of the formulation example and the comparative example was determined based on this value. Calculated.

[Table 4] Table 4

(2) Petal gray mold control test

Using petunia petals, the effects of Formulation Examples and Comparative Examples on gray mold were examined. Inoculation of gray mold was carried out by spraying an aqueous solution containing 1 × 10 ⁵ cili / g of the pathogen of gray mold on petals by spraying. Such petals were sprayed with Formulation Example 1 (2000-fold dilution), Comparative Example 1 (1000-fold dilution), and Comparative Example 6 (1000-fold dilution) diluted with water and treated.

After one day, petunia petals were examined. Table 5 shows the results.

[Table 5] Table 5

From the results in Table 5, it was confirmed that the microbial pesticide composition of the present invention has a sufficient control effect against plant diseases.

Example 5>

In the microbial pesticide composition of the present invention, it was examined whether or not drug spots on the crop surface caused by the application of the microbial pesticide composition were actually reduced.

A 1000-fold diluted solution of Formulation Example 2 and a 1000-fold diluted solution of Comparative Example 6 and eggplant (variety: Senryo No. 2) were prepared, and treated in the same manner as in Example 4 (1). Was examined. Table 6 shows the results.

[Table 6] Table 6

1: No stain due to spots is observed.

+: Slight stains due to spots are observed.

++: Clear stain due to drug spots is observed.

From the results in Table 6, it was found that the microbial pesticide composition of the present invention actually reduced the spots on the crop surface caused by the application of the microbial pesticide composition.

Industrial applicability

ADVANTAGE OF THE INVENTION According to this invention, the microbial pesticide composition which reduces the drug spot on the crop surface which arises by spraying a microbial pesticide composition, and has a sufficient plant disease control effect is provided.

Claims

The scope of the claims

[1] A microbial pesticide composition comprising a microorganism having an effect of controlling plant diseases, an oxygen-containing inorganic salt, and urea.

[2] The content of the oxygen-containing inorganic salt is 1050% by weight based on the total amount of the microorganism, the oxygen-containing inorganic salt, and the urea, and the content of the urea is the microorganism. 2. The microbial pesticide composition according to claim 1, wherein the amount is 10 to 50% by weight based on the total amount of the product, the oxygen-containing inorganic salt and the urea.

3. The method according to claim 1, wherein the content of the microorganism is 10 to 30% by weight based on the total amount of the microorganism, the oxygen-containing inorganic salt, and the urea. Biopesticide composition.

[4] A microbial pesticide composition comprising a microorganism having a plant disease controlling effect, an oxygen-containing inorganic salt, urea, and a surfactant.

[5] The content of the oxygen-containing inorganic salt is 10 to 50% by weight based on the total amount of the microorganism, the oxygen-containing inorganic salt and the urea, and the content of the urea is less than that of the microorganism, The content is 10 to 50% by weight based on the total amount of the oxygen-containing inorganic salt and the urea, and the content of the surfactant is 530% by weight based on the entire microbial pesticide composition. The microbial pesticide composition according to claim 4, characterized in that:

[6] The microorganism according to claim 4 or 5, wherein the content of the microorganism is 1030% by weight based on the total amount of the microorganism, the oxygen-containing inorganic salt and the urea. object.

7. The microbial pesticide composition according to claim 4, wherein the surfactant is an anion type.

[8] The microbial pesticide composition according to any one of [17] to [17], wherein the oxygen-containing inorganic salt is a carbonate or a sulfate.

[9] The microbial pesticide composition according to claim 8, wherein the carbonate is sodium bicarbonate.

[10] The microbial pesticide composition according to claim 8 or 9, wherein the sulfate is sodium sulfate.

[11] The microbial pesticide composition according to [11], wherein the microorganism is a bacterium belonging to the genus Bacillus.

12. The microbial pesticide composition according to claim 11, wherein the Bacillus bacterium is Bacillus subtilis.

[13] The microbial pesticide composition according to claim 11, wherein the Bacillus bacterium is NCIB12376 strain or NCIB12616 strain.